CN104430300B - The field live body method for catching of eucalyptus globulus bat moth larvae - Google Patents
The field live body method for catching of eucalyptus globulus bat moth larvae Download PDFInfo
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- CN104430300B CN104430300B CN201410760985.8A CN201410760985A CN104430300B CN 104430300 B CN104430300 B CN 104430300B CN 201410760985 A CN201410760985 A CN 201410760985A CN 104430300 B CN104430300 B CN 104430300B
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Abstract
The invention provides the field live body method for catching of a kind of eucalyptus globulus bat moth larvae, frass bag is opened, find burrow mouth, clear up the wood flour around burrow mouth and worm silk with tweezers, by raw material weight concentration 1: 1 capsicum solution dress band proboscis plastic containers, inject in eucalyptus globulus bat moth larvae burrow, burrow is filled, and frass clad is returned, to stimulate this worm to force it to climb out of from hole, lived with instrument after hole climbs out of until this worm and grabbed, and put into finger-type plastic containers bring it back into indoor standby.Time and labour saving of the present invention, the average time often catching a larva is less than 5 minutes, material therefor of the present invention is Fructus Capsici, cheap, the configuration of capsicum solution is the most simple, it is suitable for arresting in the wild the eucalyptus globulus bat moth live larva of substantial amounts of different larval instar, for screening the substantial amounts of experiment material of offer of eucalyptus globulus bat moth high virulence muscardine bacterial strain, thus reaches persistently to prevent and treat the purpose of this worm.
Description
Technical field
The invention belongs to agriculture and forestry injurious insect technical field of biological control, relate to the field live body method for catching of a kind of eucalyptus globulus bat moth larvae.
Background technology
Eucalyptus globulus bat moth (Endoclyla signifier Walker) it is a kind of main trunk borer endangering south China main yielding timber plantationsion Eucalyptus trunk energy in recent years, this worm can form one or more burrow at Eucalyptus trunk, burrow interruption-forming frass bag highlights Eucalyptus surface, affect the normal growth of Eucalyptus, it is easily caused Eucalyptus windbreak or withered time serious, causes the biggest economic loss.At present, the prevention and controls of this worm mainly based on chemical prevention, chemical prevention because environment being adversely affected and injuring the factors such as natural enemy, unsuitable excessive use, have effective biological prevention to be developed to prevent and treat this worm.
Biological prevention about this worm, good according to Yang Xiu, (the research of eucalyptus globulus bat moth Bioecological characteristics, Beijing Forestry University Ph.D. Dissertation, 2013), the infection rate of this larva field muscardine is 1.92-42.10 %, average rate 11.30 %, there is higher value of exploiting and utilizing, it is possible to use this worm is prevented and treated by muscardine.The technology of the preventing and treating most critical of muscardine is to screen the bacterial strain of high virulence, but the screening of supper toxic strain needs the live larva of a large amount of different larval instar to carry out Toxicity Determination, and the live body method for catching therefore finding the eucalyptus globulus bat moth larvae field of a kind of simple and time and labour saving is the most necessary.Owing to eucalyptus globulus bat moth larvae is using burrow as the means hiding natural enemy, larval stage is all spent in burrow, burrow will not be departed from easily, therefore want to catch live larva extremely difficult, at present in addition to the method that burrow of riving takes out the worm that lives, there are no patent and the document report of other larva live body method for catching.
Summary of the invention
The technical problem to be solved is to provide a kind of method catching eucalyptus globulus bat moth live larva for field, purpose is that the screening of the high virulence muscardine bacterial strain for eucalyptus globulus bat moth provides experiment material, lays the foundation for the research utilizing muscardine that eucalyptus globulus bat moth is carried out Biological control.
The present invention is with the following technical scheme above-mentioned technical problem of solution:
A kind of field live body method for catching of eucalyptus globulus bat moth larvae, eucalyptus globulus bat moth worm bag is torn half, find burrow mouth, the wood flour around burrow mouth and worm silk is cleared up with tweezers, plastic containers with the band proboscis equipped with liquid preparation, liquid preparation is injected in eucalyptus globulus bat moth burrow, burrow is filled capsicum solution, and worm clad is returned, wait 2 minutes of keeping quite, when having sound in worm bag, push worm bag immediately aside, rapid tweezers clamp eucalyptus globulus bat moth larvae from the incidence of eucalyptus globulus bat moth larvae, are taken out by eucalyptus globulus bat moth larvae from burrow, and put into and take back indoor in centrifuge tube.
Above-described liquid preparation is the capsicum solution of weight concentration 1: 1, its collocation method is: weigh a certain amount of fresh chilli, put in blender and add the distilled water stirring pulverizing of fresh chilli equal weight, filtering with hospital gauze and remove Fructus Capsici residue, both obtain required capsicum solution.
The wash bottle that plastic containers are 500ml capacity of above-described band mouth.
The present invention has following technological merit and a good effect:
1, this method time and labour saving.The catching method of conventional eucalyptus globulus bat moth larvae is Eucalyptus to be chopped to wood break, then is rived by burrow, and this method must cut down Eucalyptus, and easily accidentally injures the larva in burrow riving burrow when, time-consuming.The present invention is less than 5 minutes relative to former approach, saving of work and time, the time the most often catching a larva, and does not injure Eucalyptus.
2, this method is with low cost.Material therefor of the present invention is Fructus Capsici, cheap, and the configuration of capsicum solution is the most simple, is suitable for operating in the wild.
3, this method is suitable for arresting in the wild the eucalyptus globulus bat moth live larva of substantial amounts of different larval instar, for screening the substantial amounts of experiment material of offer of eucalyptus globulus bat moth high virulence muscardine bacterial strain, thus reaches persistently to prevent and treat the purpose of this worm.
Detailed description of the invention
Following example are only the present invention preferably detailed description of the invention; but protection scope of the present invention is not limited thereto; any those familiar with the art in the technical scope that the invention discloses, the change that can readily occur in or replacement, all should contain within protection scope of the present invention.
Embodiment 1
Eucalyptus globulus bat moth worm bag is torn half, finds burrow mouth, clear up the wood flour around burrow mouth and worm silk with tweezers, with equipped with the 500ml capacity band proboscis wash bottle of 1: 1 capsicum solution, capsicum solution is injected in eucalyptus globulus bat moth burrow, burrow is filled capsicum solution, and worm clad is returned, keep quite and wait 2 minutes, when having sound in worm bag, push worm bag immediately aside, clamp larva with tweezers from incidence rapidly, larva is taken out from burrow, and puts into centrifuge tube is taken back indoor.
The collocation method of above-described 1: 1 capsicum solution is: weigh a certain amount of fresh chilli, puts in blender and adds the distilled water stirring pulverizing of fresh chilli equal weight, filtering with hospital gauze and remove Fructus Capsici residue, both obtain required capsicum solution.
Claims (3)
1. the field live body method for catching of an eucalyptus globulus bat moth larvae: it is characterized in that: eucalyptus globulus bat moth worm bag is torn half, find burrow mouth, the wood flour around burrow mouth and worm silk is cleared up with tweezers, with the plastic containers of the band proboscis equipped with capsicum solution, capsicum solution is injected in eucalyptus globulus bat moth burrow, burrow is filled capsicum solution, and worm clad is returned, keep quite and wait 2 minutes, when having sound in worm bag, push worm bag immediately aside, rapid tweezers clamp eucalyptus globulus bat moth larvae from the incidence of eucalyptus globulus bat moth larvae, are taken out by eucalyptus globulus bat moth larvae from burrow, and put into and take back indoor in centrifuge tube.
The field live body method for catching of a kind of eucalyptus globulus bat moth larvae the most according to claim 1, it is characterized in that: described capsicum solution is the capsicum solution of weight concentration 1: 1, its collocation method is: weigh a certain amount of fresh chilli, put in blender and add the distilled water stirring pulverizing of fresh chilli equal weight, filter with hospital gauze and remove Fructus Capsici residue, obtain required capsicum solution.
The field live body method for catching of a kind of eucalyptus globulus bat moth larvae the most according to claim 1, it is characterised in that: the wash bottle that plastic containers are 500ml capacity of described band proboscis.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410760985.8A CN104430300B (en) | 2014-12-12 | 2014-12-12 | The field live body method for catching of eucalyptus globulus bat moth larvae |
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| CN201410760985.8A CN104430300B (en) | 2014-12-12 | 2014-12-12 | The field live body method for catching of eucalyptus globulus bat moth larvae |
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| CN104430300A CN104430300A (en) | 2015-03-25 |
| CN104430300B true CN104430300B (en) | 2016-08-17 |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104789483A (en) * | 2015-04-29 | 2015-07-22 | 广西大学 | Method for isolated culture of endoclyta signifer walker beauveria bassiana (bals.) vuill strain, and prevention and control of endoclyta signifer walkers |
| CN105176835A (en) * | 2015-09-01 | 2015-12-23 | 广西壮族自治区林业科学研究院 | Beauveria bassiana(balsamo) vuillemin strain for controlling eucalyptus locates moths, and screening and identifying method of beauveria bassiana(balsamo) vuillemin strain |
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2014
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| CN104430300A (en) | 2015-03-25 |
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Inventor after: Zou Dongxia Inventor after: Li Dewei Inventor after: Huang Naixiu Inventor after: Liao Wangjiao Inventor after: Zhu Yingzhi Inventor after: Jiang Xuejian Inventor after: Wu Yaojun Inventor after: Huang Huayan Inventor after: Luo Ji Inventor after: Chang Mingshan Inventor after: Zhao Chengjie Inventor before: Zou Dongxia Inventor before: Li Dewei Inventor before: Huang Naixiu Inventor before: Liao Wangjiao Inventor before: Zhu Yingzhi Inventor before: Jiang Xuejian Inventor before: Wu Yaojun Inventor before: Huang Huayan Inventor before: Luo Ji Inventor before: Chang Mingshan Inventor before: Zhao Chengjie |
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