CN1044199C - Dressing made of biotic products and its prodn. method - Google Patents
Dressing made of biotic products and its prodn. method Download PDFInfo
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- CN1044199C CN1044199C CN92106780A CN92106780A CN1044199C CN 1044199 C CN1044199 C CN 1044199C CN 92106780 A CN92106780 A CN 92106780A CN 92106780 A CN92106780 A CN 92106780A CN 1044199 C CN1044199 C CN 1044199C
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- biological dressing
- water
- cyclodextrin
- sus domestica
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Abstract
The present invention relates to a manufacturing method of a biological dressing, which is characterized in that the skin of a pig slaughtered before six hours is used as a raw material to remove foreign matters on a fur, subcutaneous fat is whittled away, the skin of the pig is cleaned with water containing cleanser, and then, the skin of the pig is processed through soaking, layer dissection, enzyme liquid softening, slicing, bleaching by hydrogen peroxide and disinfection by peroxyacetic acid to obtain the biological dressing. The present invention has the advantages of simple manufacturing process, low cost, long storage life, convenient use, good therapeutic effect, etc. The biological dressing is suitable for wound bandaging and wound surface covering treatment of burn, scald and wound in medical treatment.
Description
The invention belongs to the dressings in the medical apparatus and instruments, be specifically bind up a wound in the medical treatment and burn, scald, the wound surface of wound covers the production method of treatment with biological dressing.
Manufacturing biological dressing product (Corii Sus domestica) in the world the earliest is the U.S..The Genetic company of the 72 years U.S., the Araonr pharmaceutical companies is produced radiated pig skin, lyophilizing Corii Sus domestica, silver ion Corii Sus domestica in succession.After this pigskin dressing commercialization is active day by day, all establishes branch company as Genetic company in Britain, France and West Germany and produces pigskin dressing.
The lyophilizing Corii Sus domestica that radiated pig skin that domestic Shanghai Rui Jin hospital manufactured experimently in 89 years and Shanghai big Affiliated Hospital of two armies make all fails to obtain the production permit code because of lacking the commercialization standard, is in the self-produced personal state in the court.
The production technology of above-mentioned several pigskin dressings is: selects the white pig of short metre for poems that contains relatively few syllables, bark fetching, shaves hair, skiving, only get one deck epidermis and clean, again through the step handles down:
1, after the pack, low temperature is sent to the irradiation center and carries out twice roentgenization 200,000 rad sterilization, and low temperature is preserved down, is called radiated pig skin.
2, through glutaraldehyde (Glutaraldehybe) sterilization, carry out the lyophilizing pack, seal cryopreservation, be called the lyophilizing Corii Sus domestica.
3, drop in the 10 normal sulphaguanidine pyridine sodium solutions, soaked 4 days, take out and remove surface water liquid, dropped into 5 millinormal silver nitrate solutions 10 minutes, take out water flushing, pack, room temperature preservation, be called the silver ion Corii Sus domestica.
Above-mentioned three kinds of Corii Sus domestica dressing have following shortcoming according to clinical with the back reflection:
(1) hardening of easily hardening, wound surface spinosity pressure sensitivity.(2) deposit long slightly adhesiveness of phase and become inferior.(3) permeability is poor, does not beat conduction hole, easily causes the wound surface hydrops to call together and causes the infection conditions generation.(4) cause rejection sometimes.Only the silver ion pigskin dressing has anti-infectious function and room temperature to preserve outside the advantage in 1 year, and is all unsatisfactory.
Characteristics such as the objective of the invention is provides a kind of manufacture method of biological dressing in order to overcome above-mentioned shortcoming, and the biological dressing that its is produced has that technology is simple, cost is low, long shelf-life, easy to use and therapeutic effect are good.
In order to achieve the above object, the present invention is: slaughtering 6 hours is raw material with interior Corii Sus domestica, remove the foreign body on the fur, the subcutaneous fat of pruning, clean with the washing that contains detergent, then through soaking, cut open that layer, enzyme liquid are softening, cut-parts, hydrogen peroxide bleaching and peracetic acid disinfectant routine processes and make biological dressing.
The packing of biological dressing is: the medical plastic bag of packing into behind anticorrosion capsule bar of biological dressing parcel is sealed to form.Anticorrosion capsule bar is sealed to form by the medical plastic bag.Anticorrosion capsule bar is made up of medical plastic pipe and the iodo-cyclodextrin that is contained in the pipe, the manufacture method of its iodo-cyclodextrin is put into glass container for getting iodine 12, potassium iodide 9, beta-cyclodextrin 50, water 200 by weight, stirred 2 hours, left standstill then 24 hours, vacuum filtration anhydrates again, iodine and the potassium iodide by beta-the cyclodextrin bag connects removed not in water flushing for 3 times, to wrap the iodo-cyclodextrin that connects at last puts into dish and carries out drying, pulverize the back and cross the medicine sieve No. 5, its amount of iodine is that 7.0-8.5% makes.
The employed soak proportioning of Corii Sus domestica immersion process (weight with Corii Sus domestica is 100%) is: sodium sulfide 3-5%, sodium thiosulfate 2-4%, calcium hydroxide 6-7% and water 150%.
The bleaching liquid proportioning (by weight percentage) of hydrogen peroxide bleaching process accounts for 80-90% for water and hydrogen peroxide accounts for 10-20%.
The disinfectant solution proportioning (by weight percentage) of peracetic acid disinfectant process accounts for 99-99.5% for water and peracetic acid accounts for 0.5-1%.
The enzyme liquid proportioning of Corii Sus domestica enzyme liquid softening process (weight of Corii Sus domestica is 100%) is: pancreatin 0.2%, (50,000 μ) 1398 proteinase-10 .03% and water 200%.
The biological dressing that the present invention makes can make the Corii Sus domestica depilation thoroughly, and the Rhizoma Imperatae hole just is the usefulness of wound surface drain (need not to beat separately conduction hole).Reach the closure effect that does not destroy dressing like this.The present invention can the bark fetching multilamellar be a material, can reduce the dressing cost greatly.Softening process through chemical technology processing and enzyme makes the tomography Intradermal, is unfavorable for the wound healing thing, is removed minimizing, and collagenous fiber bundle is fully separated.Improved the enclosing property of dressing, reached good ventilative, poisture-penetrability, more can not harden.In the wound healing, can utilize 1 type, 3 Collagen Type VI things in the dressing again better, provide material conditions for accelerating wound healing, the present invention can also obtain the former multiple autolytic enzyme of depositing in the Corii Sus domestica removing.And, reach the preservation of long period to the greatest extent again to change the activity that pH value stops autolytic enzyme.Adopting the iodo-cyclodextrin in addition is antiseptic, very safe, reliable, can discharge iodine slowly and form saturation pressure in bag as the iodo-cyclodextrin, and to reach anticorrosion purpose, but iodine can slip into dressing again kills the wound surface antibacterial it, reaches anti-infectious function.Skin graft can be become the collagen fiber fabric in the technology, its gap only is enough to stop seeing through of antibacterial for the 11-16 dust.
The biological dressing that the present invention produces is thought through the Department of B urn of Shandong Prov. Hospital, Jinan central hospital, hydrops pool, Beijing hospital, 304 hospitals of PLA, attached institute of Nanjing Railway College of Medicine is on probation: the coverings that can be used for 3 degree wound surface of shallow 2 degree due to a variety of causes and dark 2 degree and small size are treated, and have following advantage:
(1) enough intensity and good enclosing property are arranged.(2) good adhesion.(3) good permeability need not out conduction hole in the use.(4) strong anti-infectious function is arranged.(5) closure is good.(6) effect of accelerating wound healing is arranged.(7) room temperature is deposited, and the phase of depositing reaches 18 months.(8) transparency is good, intuitively the wound surface situation.(9) obvious analgesic effect is arranged.(10) easy to use, reduce dressing change frequency.(11) generation of avirulence and irritated situation.It is present comparatively ideal wound surface covering material.
Embodiments of the invention are:
Get fresh pig skin and remove the foreign body on the fur for raw material (limit is slaughtered in back 6 hours) for 10 kilograms, the subcutaneous fat of pruning is sent in the rustless steel agitator that soak is housed only with the washing that contains detergent, handles 6 hours.The soak proportioning is: 0.4 kilogram in sodium sulfide, 0.3 kilogram in sodium thiosulfate, 0.6 kilogram of calcium hydroxide, 15 kilograms in water.During processing, be interrupted stirring, every 2 hours once, each 5 minutes, per minute stirred 3-4 time.In the time of 6 hours (hair comes off, dissolves, and suction thicken be former thick 1.5-3 doubly) the venting immersion, changing water cleans, cut open layer (thickness) by 0.4-0.8mm again, layer is got the tomography skin that contains collagen, range estimation eligible (thickness is even, nothing is damaged) is clean to wash, send into rustless steel container, the enzyme bating liquid of packing in the container, stir process 2 hours.The dosing ratio is: 0.02 kilogram of pancreatin, (50,000 μ) 1398 proteinase-10 .003 kilograms, 20 kilograms in water, is regulated PH7.55-8 by temperature control 37-38 ℃.The 1398 neutral proteinase product that 1398 protease adopt second chemical plant, Zhaoyuan, Shandong to produce.
It is clean to change washing, is cut into skin graft by the specification size again.
Water that the interior input of rustless steel container is 8 kilograms and 2 kilograms hydrogen peroxide are mixed into bleaching liquid, then skin graft are put into bleaching liquid and soak 2 hours, and it is pure white to look skin graft.Drop into 9.9 kilograms water and 0.1 kilogram peracetic acid again and stir into disinfectant solution in another rustless steel container, the skin graft after the bleaching is put into disinfectant solution immersion 15 minutes, skin graft just becomes biological dressing.With a certain amount of dressing anticorrosion capsule bar of iodo-cyclodextrin of packing into simultaneously in the medical PVC bag of packing into, seal and be finished product, qualified warehouse-in.
The compound method of iodo-cyclodextrin is got iodine 240 grams, potassium iodide 180 grams; Beta-cyclodextrin 1000 grams; 4000 milliliters in water is put into glass container, fully stirs 2 hours, leaves standstill 24 hours, and vacuum filtration anhydrates, and with water flushing 3 times, removes not iodine and potassium iodide by beta-the cyclodextrin bag connects.
To put into that dish carries out drying, pulverized No. 5 medicines sieve, surveys its amount of iodine be 7.8-8.5% with wrapping the iodo-cyclodextrin that connects, and the certified products of umbrinaceous iodo-cyclodextrin are sealed in bottle again and store standby.
Claims (6)
1, a kind of bind up a wound and burn, scald, the wound surface of wound surface covers the production method of treatment with biological dressing, feature of the present invention is: slaughtering 6 hours is raw material with interior Corii Sus domestica, remove the foreign body on the fur, the subcutaneous fat of pruning, clean with the washing that contains detergent, layer enzyme liquid is softening through soaking, cuing open then, cut-parts, hydrogen peroxide bleaching and peracetic acid disinfectant routine processes and make biological dressing.
2, the production method of biological dressing according to claim 1, the packing of the biological dressing that it is produced is that the medical plastic bag of packing into behind anticorrosion capsule bar of biological dressing parcel is sealed to form, anticorrosion capsule bar is made up of medical plastic pipe and the iodo-cyclodextrin that is contained in the pipe, the manufacture method that it is characterized in that the iodo-cyclodextrin is for getting iodine 12 by weight, potassium iodide 9, beta-cyclodextrin 50, water 200 is put into glass container and was stirred 2 hours, left standstill then 24 hours, vacuum filtration anhydrates again, iodine and the potassium iodide by beta-the cyclodextrin bag connects removed not in water flushing for 3 times, the iodo-cyclodextrin that at last bag is connect is put into dish and is carried out drying, pulverize the back and cross the medicine sieve No. 5, its amount of iodine is that 7.8-8.5% makes.
3, the production method of biological dressing according to claim 1 is characterized in that the employed soak of said Corii Sus domestica immersion process (Corii Sus domestica heavily be 100%) proportioning is sodium sulfide 3-5%, sodium thiosulfate 2-4%, calcium hydroxide 6-7% and water 150%.
4, the production method of biological dressing according to claim 1 is characterized in that enzyme liquid (weight of Corii Sus domestica the is 100%) proportioning of said Corii Sus domestica enzyme liquid softening process is: pancreatin 0.2%, (50,000 μ) 1398 proteinase-10 .03% and water 200%.
5, the production method of biological dressing according to claim 1, the bleaching liquid proportioning (by weight percentage) that it is characterized in that said hydrogen peroxide bleaching process accounts for 80-90% for water and hydrogen peroxide accounts for 10-20%.
6, the production method of biological dressing according to claim 1, the disinfectant solution proportioning (by weight percentage) that it is characterized in that said peracetic acid disinfectant process accounts for 99-99.5% for water and peracetic acid accounts for 0.5-1%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN92106780A CN1044199C (en) | 1992-10-23 | 1992-10-23 | Dressing made of biotic products and its prodn. method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN92106780A CN1044199C (en) | 1992-10-23 | 1992-10-23 | Dressing made of biotic products and its prodn. method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1085807A CN1085807A (en) | 1994-04-27 |
| CN1044199C true CN1044199C (en) | 1999-07-21 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN92106780A Expired - Fee Related CN1044199C (en) | 1992-10-23 | 1992-10-23 | Dressing made of biotic products and its prodn. method |
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| Country | Link |
|---|---|
| CN (1) | CN1044199C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100427154C (en) * | 2005-01-27 | 2008-10-22 | 天津市托福医用原子能科技有限公司 | SD-Zn irradiated biological skin and its prepn process |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100415309C (en) * | 2003-07-03 | 2008-09-03 | 中国人民解放军第三○四医院 | Application of selective cell-removed pork skin as skin substitute of human body and its preparation method |
| DE102011008604A1 (en) | 2011-01-14 | 2012-07-19 | Tutogen Medical Gmbh | Preparation of a graft of animal dermis with sodium sulfide solution |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1060611A (en) * | 1991-05-14 | 1992-04-29 | 扬州生物化学制药厂 | The processing method of primary sterilization iodine bag pigskin dressing |
-
1992
- 1992-10-23 CN CN92106780A patent/CN1044199C/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1060611A (en) * | 1991-05-14 | 1992-04-29 | 扬州生物化学制药厂 | The processing method of primary sterilization iodine bag pigskin dressing |
Non-Patent Citations (1)
| Title |
|---|
| 中国中西医现代研究丛书分册"烧伤医学在中国" 1989.1.1 史济湘，邝安 ，湖南科技出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100427154C (en) * | 2005-01-27 | 2008-10-22 | 天津市托福医用原子能科技有限公司 | SD-Zn irradiated biological skin and its prepn process |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1085807A (en) | 1994-04-27 |
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