CN104415801B - A kind of portable microlayer model generator based on self energizing vacuum Micropump - Google Patents
A kind of portable microlayer model generator based on self energizing vacuum Micropump Download PDFInfo
- Publication number
- CN104415801B CN104415801B CN201310382774.0A CN201310382774A CN104415801B CN 104415801 B CN104415801 B CN 104415801B CN 201310382774 A CN201310382774 A CN 201310382774A CN 104415801 B CN104415801 B CN 104415801B
- Authority
- CN
- China
- Prior art keywords
- microlayer model
- model generator
- portable
- self energizing
- generator based
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Micromachines (AREA)
Abstract
Wood invention relates to the equipment preparing microlayer model, specifically a kind of portable microlayer model generator based on self energizing vacuum Micropump.Comprise the cover plate with micro-structural setting up and down and flat plate substrate; described there is micro-structural cover plate and flat plate substrate be tightly connected; form suction chamber, control room, fluid microchannels and gas microchannel having between the cover plate of micro-structural and flat plate substrate; one end of described fluid microchannels is provided with aqueous phase solution entrance and oil-phase solution entrance; the other end is connected with suction chamber, and described control room is connected with suction chamber by gas microchannel; Described microlayer model generator, through vacuum outgas, forms the generation that negative pressure of vacuum environment is microlayer model and provides driving force in fluid microchannels or suction chamber, is switched the airtight and open state of microlayer model generator, realize generation and the stopping of microlayer model by control room.Wood invention have assembling simple, be easy to operate, portable feature, show the application potential that it is huge in instant analysis at the scene.
Description
Technical field
Wood invention relates to the equipment preparing microlayer model, specifically a kind of portable microlayer model generator based on self energizing vacuum Micropump.
Degassed dimethyl silicone polymer vacuum microenvironment of being formed in the microchannel closed is utilized as driving force, to build self energizing, portable microlayer model generator.
Background technology
Microlayer model utilizes water, the immiscible characteristic of oily two-phase, orders about lower formation in external force and interfacial tension.Tradition microlayer model preparation method relies on strong stirring, vibration, maybe repeatedly presses through fenestra by what be pre-formed drop.The defects such as it is uncontrolled that these methods exist droplet size, monodispersity difference.In recent years, the new method adopting microfluidic system to prepare microlayer model is proposed.It not only can prepare high monodispersed drop (variation of droplet size is generally less than 3%), and can manipulate neatly drop fusion, divide, catch and sorting etc.Due to micro-fluidic microlayer model chip have sample consumption less, mixing velocity soon, not easily causes cross pollution and is easy to the advantages such as manipulation, at the synthesis of new material, the cultivation of cell and screening, crystallization of protein, in the fields such as the detection of biomarker, there is important application.
At present, method micro-fluidic chip being prepared microlayer model mainly comprises: light field, electric field and mechanical pump.These methods can high flux ground, controllably produce highly monodispersed drop, but be confined to need outside powering device, be not suitable for for portable analysis and detection.Rely on the static pressure of fluid column to drive the generation of microlayer model, although solve the problem of outside energy supply, the pipeline of complexity connects the dead volume of the system that significantly increases.Weitz research group reports a kind of microlayer model generator that can be portable, and it utilizes syringe to vacuumize and provides negative pressure.But this microlayer model generator needs the size regulating and controlling drop by monofilm valve in real time.
Summary of the invention
For the problems referred to above, the object of wood invention is to provide a kind of portable microlayer model generator based on self energizing vacuum Micropump.This portable microlayer model generator have assembling simple, be easy to operate, portable feature.
To achieve these goals, wood invention is by the following technical solutions:
A kind of portable microlayer model generator based on self energizing vacuum Micropump; comprise the cover plate with micro-structural setting up and down and flat plate substrate; described there is micro-structural cover plate and flat plate substrate be tightly connected; form suction chamber, control room, fluid microchannels and gas microchannel having between the cover plate of micro-structural and flat plate substrate; one end of described fluid microchannels is provided with aqueous phase solution entrance and oil-phase solution entrance; the other end is connected with suction chamber, and described control room is connected with suction chamber by gas microchannel; Described microlayer model generator, through vacuum outgas, forms the generation that negative pressure of vacuum environment is microlayer model and provides driving force in fluid microchannels or suction chamber, is switched the airtight and open state of microlayer model generator, realize generation and the stopping of microlayer model by control room.
The described cover plate with micro-structural is dimethyl silicone polymer.Described flat plate substrate is glass, polymer, silicon materials or metal.The described microlayer model generator vacuum outgas time is 5 minutes to 12 hours.
The size of described microlayer model is regulated by fluid resistance or hydraulic pressure.Described fluid resistance regulates by changing the width of fluid microchannels, height or length, and the width of described fluid microchannels is 10-500 μm, is highly 10-500 μm, and length is 1-100mm.Described hydraulic pressure is regulated by the liquid-column height of the aqueous phase solution that adds or oil-phase solution, and the liquid-column height of described aqueous phase solution or oil-phase solution is 1-100mm.
Described control room is provided with film, realizes the switching of the airtight or open state of described microlayer model generator by covering or open film.
Advantage and the beneficial effect of wood invention are:
1. wood invention is the driving force that the negative pressure of vacuum environment utilizing degassed PDMS (dimethyl silicone polymer) to be formed in the microchannel closed produces as drop, avoid outside energy supply to be connected with complicated stream, realize the preparation of highly controlled single dispersing drop.
2. wood invention have assembling simple, be easy to operate, portable feature, show the application potential that it is huge in instant analysis at the scene.
Accompanying drawing explanation
Fig. 1 is the structural representation of wood invention;
Fig. 2 is the microlayer model image that wood invention produces.
Wherein: 1 for having the cover plate of micro-structural, 2 is flat plate substrate, and 3 is aqueous phase solution entrance, and 4 is oil-phase solution entrance, and 5 is suction chamber, and 6 is control room, and 7 is film, and 8 is fluid microchannels, and 9 is gas microchannel.
Detailed description of the invention
Below in conjunction with drawings and Examples, wood invention is further described.
As shown in Figure 1, wood invention comprises the cover plate 1 with micro-structural setting up and down and flat plate substrate 2, the described cover plate 1 with micro-structural is tightly connected with flat plate substrate 2, form suction chamber 5, control room 6, fluid microchannels 8 and gas microchannel 9 having between the cover plate 1 of micro-structural and flat plate substrate 2, one end of described fluid microchannels 8 is provided with aqueous phase solution entrance 3 and oil-phase solution entrance 4, the other end is connected with suction chamber 5, and described control room 6 is connected with suction chamber 5 by multiple gas microchannel 9.Described microlayer model generator, through vacuum outgas, forms the generation that negative pressure of vacuum environment is microlayer model and provides driving force, avoid outside energy supply and be connected with complicated stream in fluid microchannels 8 or suction chamber 5.Generation and the stopping of microlayer model are realized by the airtight or open state of described microlayer model generator.Described control room 6 is provided with film 7, and the switching of the airtight and open state of described microlayer model generator realizes by covering or open film 7, thus realizes the preparation of highly controlled single dispersing drop.
The described cover plate 1 with micro-structural is dimethyl silicone polymer.Described flat plate substrate 2 is glass, polymer, silicon materials or metal.The described microlayer model generator vacuum outgas time is 5 minutes to 12 hours.
The size of described microlayer model is regulated by fluid resistance or hydraulic pressure.Described fluid resistance regulates by changing the width of fluid microchannels 8, height or length, and the width of described fluid microchannels 8 is 10-500 μm, is highly 10-500 μm, and length is 1-100mm.Described hydraulic pressure is regulated by the liquid-column height of the aqueous phase solution that adds or oil-phase solution, and the liquid-column height of described aqueous phase solution or oil-phase solution is 1-100mm.
Embodiment
The making of the described micro-structural had on the cover plate 1 of micro-structural is: first utilize optical lithography to make SU-8 template, then dimethyl silicone polymer is cast in template, 80 DEG C of baking ovens are polymerized 2 hours, are finally had by the marking polymer of micro-structural to peel off from mould.Flat plate substrate 2 is plate glass, has cover plate 1 and flat plate substrate 2 oxygen plasma treatment of micro-structural, seals formation microfluidic channel.The chip of sealing-in is placed in 80 DEG C of baking ovens to spend the night, makes dimethyl silicone polymer recover its hydrophobicity.Before use microlayer model generator is placed in vacuum desiccator degassed, quick aqueous phase solution and oil-phase solution being dripped drips in microlayer model generator from aqueous phase solution entrance 3 and oil-phase solution entrance 4 respectively afterwards.In fluid microchannels 8 and suction chamber 5 negative pressure effect under, microlayer model formed.The size of the microlayer model that result display produces is homogeneous, and its coefficient of variation is 2.2%, as shown in Figure 2.
Claims (9)
1. the portable microlayer model generator based on self energizing vacuum Micropump, it is characterized in that: comprise the cover plate (1) with micro-structural setting up and down and flat plate substrate (2), the described cover plate (1) with micro-structural is tightly connected with flat plate substrate (2), form suction chamber (5) having between the cover plate (1) of micro-structural and flat plate substrate (2), control room (6), fluid microchannels (8) and gas microchannel (9), one end of described fluid microchannels (8) is provided with aqueous phase solution entrance (3) and oil-phase solution entrance (4), the other end is connected with suction chamber (5), described control room (6) is connected with suction chamber (5) by gas microchannel (9), described microlayer model generator is through vacuum outgas, in fluid microchannels (8) or suction chamber (5), form the generation that negative pressure of vacuum environment is microlayer model driving force is provided, switched the airtight and open state of microlayer model generator by control room (6), realize generation and the stopping of microlayer model, the size of described microlayer model is regulated by fluid resistance or hydraulic pressure.
2., by the portable microlayer model generator based on self energizing vacuum Micropump according to claim 1, it is characterized in that: described in there is micro-structural cover plate (1) be dimethyl silicone polymer.
3., by the portable microlayer model generator based on self energizing vacuum Micropump according to claim 1, it is characterized in that: described flat plate substrate (2) is glass, polymer, silicon materials or metal.
4., by the portable microlayer model generator based on self energizing vacuum Micropump according to claim 1, it is characterized in that: the described microlayer model generator vacuum outgas time is 5 minutes to 12 hours.
5. by the portable microlayer model generator based on self energizing vacuum Micropump according to claim 1, it is characterized in that: described fluid resistance is regulated by the width of change fluid microchannels (8), height or length.
6., by the portable microlayer model generator based on self energizing vacuum Micropump according to claim 5, it is characterized in that: the width of described fluid microchannels (8) is 10-500 μm, is highly 10-500 μm, and length is 1-100mm.
7. by the portable microlayer model generator based on self energizing vacuum Micropump according to claim 1, it is characterized in that: described hydraulic pressure is regulated by the liquid-column height of the aqueous phase solution that adds or oil-phase solution.
8., by the portable microlayer model generator based on self energizing vacuum Micropump according to claim 7, it is characterized in that: the liquid-column height of described aqueous phase solution or oil-phase solution is 1-100mm.
9. by the portable microlayer model generator based on self energizing vacuum Micropump according to claim 1, it is characterized in that: described control room (6) are provided with film (7), realize the switching of the airtight or open state of described microlayer model generator by covering or open film (7).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310382774.0A CN104415801B (en) | 2013-08-28 | 2013-08-28 | A kind of portable microlayer model generator based on self energizing vacuum Micropump |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310382774.0A CN104415801B (en) | 2013-08-28 | 2013-08-28 | A kind of portable microlayer model generator based on self energizing vacuum Micropump |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104415801A CN104415801A (en) | 2015-03-18 |
| CN104415801B true CN104415801B (en) | 2016-03-09 |
Family
ID=52966579
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310382774.0A Active CN104415801B (en) | 2013-08-28 | 2013-08-28 | A kind of portable microlayer model generator based on self energizing vacuum Micropump |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104415801B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106148159A (en) * | 2015-03-23 | 2016-11-23 | 西南大学 | A kind of fast-growth microalgae algae plant height throughput screening systems and method |
| CN106563517A (en) * | 2016-10-26 | 2017-04-19 | 杭州霆科生物科技有限公司 | Micro-fluidic chip and detection system for detecting formaldehyde and pH value of textile |
| CN108080046A (en) * | 2018-01-29 | 2018-05-29 | 苏州昊通仪器科技有限公司 | Drop formation apparatus and method |
| CN108514895A (en) * | 2018-03-01 | 2018-09-11 | 北京天健惠康生物科技有限公司 | A kind of microlayer model generates and monitoring device |
| CN109231148A (en) * | 2018-09-25 | 2019-01-18 | 苏州大学 | Micro-pump structure based on room temperature liquid metal |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2034317A1 (en) * | 2006-06-12 | 2009-03-11 | Konica Minolta Medical & Graphic, Inc. | Micro general analysis system with mechanism for preventing leakage of liquid |
| CN101561448A (en) * | 2008-04-18 | 2009-10-21 | 中国科学院大连化学物理研究所 | Negative-pressure pinched injection method of micro-fluidic chip based on integrated minipump valve and special chip thereof |
| CN101570776A (en) * | 2008-04-29 | 2009-11-04 | 中国科学院大连化学物理研究所 | High-throughput nano-litre micro-droplet forming and fixing method based on microfluidic chip and special chip and application thereof |
| CN101590389A (en) * | 2009-06-24 | 2009-12-02 | 中国科学院大连化学物理研究所 | Drop formation and catching method and application thereof based on three-layer sandwich disc-type chip |
| CN102161967A (en) * | 2010-02-24 | 2011-08-24 | 中国科学院大连化学物理研究所 | Microfluidic-based nucleic acid hybridization reaction platform and hybridization analysis method |
| CN102174395A (en) * | 2011-01-30 | 2011-09-07 | 中国科学院广州生物医药与健康研究院 | Automation augmentation and culture system of induced pluripotent stem cells |
| CN102259040A (en) * | 2010-05-26 | 2011-11-30 | 中国科学院大连化学物理研究所 | High-throughput micro-droplet fixing method and special micro-fluidic chip thereof |
| CN102418684A (en) * | 2011-08-19 | 2012-04-18 | 中国科学院上海微系统与信息技术研究所 | Modular assembled micropump as well as use method and application thereof |
| CN102530830A (en) * | 2010-12-24 | 2012-07-04 | 中国科学院大连化学物理研究所 | Integrated micro-valve droplet control chip and method for synthesizing and assembling polymer microspheres |
-
2013
- 2013-08-28 CN CN201310382774.0A patent/CN104415801B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2034317A1 (en) * | 2006-06-12 | 2009-03-11 | Konica Minolta Medical & Graphic, Inc. | Micro general analysis system with mechanism for preventing leakage of liquid |
| CN101561448A (en) * | 2008-04-18 | 2009-10-21 | 中国科学院大连化学物理研究所 | Negative-pressure pinched injection method of micro-fluidic chip based on integrated minipump valve and special chip thereof |
| CN101570776A (en) * | 2008-04-29 | 2009-11-04 | 中国科学院大连化学物理研究所 | High-throughput nano-litre micro-droplet forming and fixing method based on microfluidic chip and special chip and application thereof |
| CN101590389A (en) * | 2009-06-24 | 2009-12-02 | 中国科学院大连化学物理研究所 | Drop formation and catching method and application thereof based on three-layer sandwich disc-type chip |
| CN102161967A (en) * | 2010-02-24 | 2011-08-24 | 中国科学院大连化学物理研究所 | Microfluidic-based nucleic acid hybridization reaction platform and hybridization analysis method |
| CN102259040A (en) * | 2010-05-26 | 2011-11-30 | 中国科学院大连化学物理研究所 | High-throughput micro-droplet fixing method and special micro-fluidic chip thereof |
| CN102530830A (en) * | 2010-12-24 | 2012-07-04 | 中国科学院大连化学物理研究所 | Integrated micro-valve droplet control chip and method for synthesizing and assembling polymer microspheres |
| CN102174395A (en) * | 2011-01-30 | 2011-09-07 | 中国科学院广州生物医药与健康研究院 | Automation augmentation and culture system of induced pluripotent stem cells |
| CN102418684A (en) * | 2011-08-19 | 2012-04-18 | 中国科学院上海微系统与信息技术研究所 | Modular assembled micropump as well as use method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104415801A (en) | 2015-03-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104415801B (en) | A kind of portable microlayer model generator based on self energizing vacuum Micropump | |
| Au et al. | Microvalves and micropumps for BioMEMS | |
| Wang et al. | Artificial cilia fabricated using magnetic fiber drawing generate substantial fluid flow | |
| Zhang et al. | A portable plug-and-play syringe pump using passive valves for microfluidic applications | |
| Orbay et al. | Acoustic actuation of in situ fabricated artificial cilia | |
| CN203925955U (en) | A kind of electromagnetism Micropump based on micro-fluidic chip | |
| Li et al. | A bio-inspired micropump based on stomatal transpiration in plants | |
| CN102876563B (en) | Micro- fluid control chip capable of automatically catching single cells | |
| Chan et al. | A bubble-activated micropump with high-frequency flow reversal | |
| Oh et al. | A microfluidic chaotic mixer using ferrofluid | |
| CN103816805A (en) | Electroosmotic micropump device | |
| Rahbar et al. | Design, fabrication and characterization of an arrayable all-polymer microfluidic valve employing highly magnetic rare-earth composite polymer | |
| Oskooei et al. | Bubble gate for in-plane flow control | |
| Chuang et al. | Bio-O-Pump: A novel portable microfluidic device driven by osmotic pressure | |
| Zhang et al. | An integrated micro-millifluidic processing system | |
| Li et al. | A micropump based on water potential difference in plants | |
| Kumar et al. | Development of a solenoid actuated planar valveless micropump with single and multiple inlet–outlet arrangements | |
| CN103801415A (en) | Button type microfluid control system and button type microfluid control method | |
| Ni et al. | A pneumatic PDMS micropump with in-plane check valves for disposable microfluidic systems | |
| Tsai et al. | Digital hydraulic drive for microfluidics and miniaturized cell culture devices based on shape memory alloy actuators | |
| CN110343611B (en) | Micro-fluidic chip | |
| Nagai et al. | Characterization of light-controlled Volvox as movable microvalve element assembled in multilayer microfluidic device | |
| Peng | A one-square-millimeter compact hollow structure for microfluidic pumping on an all-glass chip | |
| CN102588256B (en) | Single-layer dual-cavity vacuum drive peristaltic pump with automatically-closed one-way valve | |
| CN103573592B (en) | Conical electroactive-polymer-driven single-chamber micro-pump |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |