CN104404017A - Complex enzyme preparation and application of complex enzyme preparation to pulping and papermaking - Google Patents

Complex enzyme preparation and application of complex enzyme preparation to pulping and papermaking Download PDF

Info

Publication number
CN104404017A
CN104404017A CN201410780614.6A CN201410780614A CN104404017A CN 104404017 A CN104404017 A CN 104404017A CN 201410780614 A CN201410780614 A CN 201410780614A CN 104404017 A CN104404017 A CN 104404017A
Authority
CN
China
Prior art keywords
enzyme preparation
complex enzyme
parts
pulping
papermaking
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201410780614.6A
Other languages
Chinese (zh)
Inventor
甘鲜
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GUANGZHOU UNIKBIO BIOTECHNOLOGY CO Ltd
Original Assignee
GUANGZHOU UNIKBIO BIOTECHNOLOGY CO Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GUANGZHOU UNIKBIO BIOTECHNOLOGY CO Ltd filed Critical GUANGZHOU UNIKBIO BIOTECHNOLOGY CO Ltd
Priority to CN201410780614.6A priority Critical patent/CN104404017A/en
Publication of CN104404017A publication Critical patent/CN104404017A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0055—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
    • C12N9/0057—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10) with oxygen as acceptor (1.10.3)
    • C12N9/0061—Laccase (1.10.3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y110/00—Oxidoreductases acting on diphenols and related substances as donors (1.10)
    • C12Y110/03—Oxidoreductases acting on diphenols and related substances as donors (1.10) with an oxygen as acceptor (1.10.3)
    • C12Y110/03002—Laccase (1.10.3.2)
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21D—TREATMENT OF THE MATERIALS BEFORE PASSING TO THE PAPER-MAKING MACHINE
    • D21D1/00—Methods of beating or refining; Beaters of the Hollander type
    • D21D1/02—Methods of beating; Beaters of the Hollander type

Abstract

The invention discloses a complex enzyme preparation and application of complex enzyme preparation to pulping and papermaking. The complex enzyme preparation comprises the following components in parts by weight: 50-55 parts of xylanase, 10-12 parts of cellulose, 10-13 parts of pectinase, 10-13 parts of laccase, 10-13 parts of amylase and 10-13 parts of hemicellulase. By virtue of the complex enzyme preparation, the quality of the produced paper can be improved; the use amount of long fibers can be reduced; the cost can be reduced; the complex enzyme preparation can be used for improving fibrillation of fibers and saving energy in the pulping and papermaking process; the complex enzyme preparation is added into a pulp crusher to pulp; the pulping energy consumption is reduced; the service life of a grinding blade is prolonged; the pulping capability is improved.

Description

A kind of compound enzymic preparation and the application in papermaking making beating thereof
Technical field
The invention belongs to industrial biotechnology field, particularly a kind of compound enzymic preparation and the application in papermaking making beating thereof.
Background technology
making beating is the paper pulp fiber utilizing mechanical effect process to suspend in water, and makes it have adaptation and produces required characteristic on a papermaking machine, and makes produced paper can reach the operating process of prospective quality.Utilize physical method, carry out machinery or fluid handling to paper pulp fiber in water, make fiber be subject to shearing force, change the form of fiber, make paper pulp obtain some characteristic, to ensure that the paper be copied into reaches the specification of quality of expection, this process is just referred to as " making beating ".
In pulping process fiber except by parts shearing, rub with except the effect such as combing, simultaneously the cell walls of fiber be also subjected to displacement, the phenomenon such as deformation and failure and the swollen that absorbs water, produce fibrillation, paper pulp is made to have flexibility, plasticity-, also make the hydroxyl in cellulose molecular chain increase and hydrogen chain combination chance, improve interfibrous bonding force.By making beating effect, shiny beating and free beating can be divided into.By the mode of production, interval making beating and continuous beating can be divided into.
Summary of the invention
Technical problem to be solved by this invention is the difficulty overcoming prior art, and object is that providing a kind of is added in pulping engine for production of pulling an oar, and effectively can carry making beating efficiency compound enzymic preparation.
Another object of the present invention is to provide the application of above-mentioned compound enzymic preparation in making beating.
The present invention is achieved through the following technical solutions above-mentioned purpose:
A kind of compound enzymic preparation, is made up of the component of following weight parts: zytase 50 ~ 55, cellulase 10 ~ 12, polygalacturonase 10 ~ 13, laccase 10 ~ 13, amylase 10 ~ 13, hemicellulase 10 ~ 13 form.
Warm amylase during above-mentioned amylase is preferred.
The preparation method of compound enzymic preparation of the present invention is: each component is carried out mixing composite, then after packaging, becomes product.
The top condition that compound enzymic preparation of the present invention is had an effect is: natural solarization system fermentation, and temperature, more than 30 DEG C, has sufficient solar radiation; Heat-preservation fermentation, homo(io)thermism is at 45 DEG C.
Prozyme of the present invention can be applied in refining process, improve fiber wire-dividing broom purification, in energy efficient, by described prozyme system, add pulping engine to after making beating, reduce beating energy consumption, extend diaphragm work-ing life, improve making beating ability.
Compared with prior art, the present invention has following beneficial effect:
(1) prozyme of the present invention can improve into paper product matter, reduces long stapled consumption, cost-saving;
(2) prozyme of the present invention is applied in refining process, can improve the wire-dividing broom purification of fiber, energy efficient, extends diaphragm work-ing life, improves making beating ability.
Embodiment
The invention provides a kind of compound enzymic preparation be applied in papermaking making beating, this compound enzymic preparation is made up of the component of following weight percent: zytase 50 ~ 55, cellulase 10 ~ 12, polygalacturonase 10 ~ 13, laccase 10 ~ 13, amylase 10 ~ 13, hemicellulase 10 ~ 13 form.
By described prozyme system, add pulping engine to after making beating, reduce beating energy consumption, extend diaphragm work-ing life, improve making beating ability.
Explain the present invention further below in conjunction with specific embodiment, but embodiment does not limit in any form to the present invention.
Embodiment 1
Zytase 50, cellulase 10, polygalacturonase 10, laccase 10, amylase 10, hemicellulase 10.
Embodiment 2
Zytase 55, cellulase 12, polygalacturonase 13, laccase 13, amylase 13, hemicellulase 13.
Embodiment 3
Zytase 52.5, cellulase 11, polygalacturonase 11.5, laccase 11.5, amylase 11.5, hemicellulase 11.5.
Embodiment 4
Zytase 51, cellulase 10.5, polygalacturonase 10.5, laccase 10.5, amylase 10.5, hemicellulase 10.5.
Embodiment 5
Zytase 54, cellulase 11 .5, polygalacturonase 11.5, laccase 11.5, amylase 11.5, hemicellulase 11.5.

Claims (4)

1. a compound enzymic preparation, is characterized in that being made up of the following component according to weight parts: zytase 50 ~ 55, cellulase 10 ~ 12, polygalacturonase 10 ~ 13, laccase 10 ~ 13, amylase 10 ~ 13, hemicellulase 10 ~ 13 form.
2. compound enzymic preparation according to claim 1, is characterized in that described amylase is middle temperature amylase.
3. the application of compound enzymic preparation described in claim 1 in refining process.
4. application according to claim 3, is characterized in that, in pulping process, described compound enzymic preparation directly or after dilution is added pulping engine.
CN201410780614.6A 2014-12-17 2014-12-17 Complex enzyme preparation and application of complex enzyme preparation to pulping and papermaking Pending CN104404017A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201410780614.6A CN104404017A (en) 2014-12-17 2014-12-17 Complex enzyme preparation and application of complex enzyme preparation to pulping and papermaking

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201410780614.6A CN104404017A (en) 2014-12-17 2014-12-17 Complex enzyme preparation and application of complex enzyme preparation to pulping and papermaking

Publications (1)

Publication Number Publication Date
CN104404017A true CN104404017A (en) 2015-03-11

Family

ID=52641686

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201410780614.6A Pending CN104404017A (en) 2014-12-17 2014-12-17 Complex enzyme preparation and application of complex enzyme preparation to pulping and papermaking

Country Status (1)

Country Link
CN (1) CN104404017A (en)

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105735035A (en) * 2016-03-02 2016-07-06 福建希源纸业有限公司 Method for producing base wallpaper from waste paper deinking pulp
CN105908562A (en) * 2016-06-21 2016-08-31 福建省晋江优兰发纸业有限公司 Method for producing translucent paper through beating enzymes
CN105951523A (en) * 2016-06-27 2016-09-21 福建省晋江优兰发纸业有限公司 Production method of low gram weight copy paper
CN106011120A (en) * 2016-06-18 2016-10-12 颐思特（武汉）生物科技有限公司 Plant fiber modified compound enzyme preparation and preparation method and application thereof
CN106007187A (en) * 2016-06-28 2016-10-12 福建省晋江优兰发纸业有限公司 High-end packaging paper production waste water reuse and treatment method
CN106120460A (en) * 2016-06-30 2016-11-16 福建省晋江优兰发纸业有限公司 A kind of sulphite produces the method for high transparency paper
CN106149434A (en) * 2016-06-30 2016-11-23 福建省晋江优兰发纸业有限公司 A kind of grinding method of high beating degree copy paper
CN109810964A (en) * 2017-11-21 2019-05-28 湖南海正生物科技有限公司 A kind of mixing enzyme preparation and preparation method thereof for mashing
CN112375753A (en) * 2020-11-27 2021-02-19 苏州埃斯腾特生物科技有限公司 Pulping enzyme preparation for electrostatic copying paper and preparation method and application thereof
CN113355311A (en) * 2020-08-14 2021-09-07 嘉兴景和环保科技有限公司 Biological complex enzyme preparation for softening wood fibers and preparation method thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103572632A (en) * 2012-07-31 2014-02-12 金东纸业（江苏）股份有限公司 Slurry treatment method and slurry prepared from method
CN103937768A (en) * 2014-02-14 2014-07-23 济南诺能生物工程有限公司 Preparation method of energy-saving papermaking compound enzyme

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103572632A (en) * 2012-07-31 2014-02-12 金东纸业（江苏）股份有限公司 Slurry treatment method and slurry prepared from method
CN103937768A (en) * 2014-02-14 2014-07-23 济南诺能生物工程有限公司 Preparation method of energy-saving papermaking compound enzyme

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
何晓红等: "不同生物酶处理对黄麻纤维的影响", 《纺织科技进展》 *
邰文峰等: "亚麻/棉织物生物酶前处理工艺的研究", 《山东纺织科技》 *

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105735035A (en) * 2016-03-02 2016-07-06 福建希源纸业有限公司 Method for producing base wallpaper from waste paper deinking pulp
CN106011120A (en) * 2016-06-18 2016-10-12 颐思特（武汉）生物科技有限公司 Plant fiber modified compound enzyme preparation and preparation method and application thereof
CN105908562A (en) * 2016-06-21 2016-08-31 福建省晋江优兰发纸业有限公司 Method for producing translucent paper through beating enzymes
CN105951523A (en) * 2016-06-27 2016-09-21 福建省晋江优兰发纸业有限公司 Production method of low gram weight copy paper
CN106007187A (en) * 2016-06-28 2016-10-12 福建省晋江优兰发纸业有限公司 High-end packaging paper production waste water reuse and treatment method
CN106120460A (en) * 2016-06-30 2016-11-16 福建省晋江优兰发纸业有限公司 A kind of sulphite produces the method for high transparency paper
CN106149434A (en) * 2016-06-30 2016-11-23 福建省晋江优兰发纸业有限公司 A kind of grinding method of high beating degree copy paper
CN106149434B (en) * 2016-06-30 2018-01-23 福建省晋江优兰发纸业有限公司 A kind of grinding method of high beating degree copy paper
CN106120460B (en) * 2016-06-30 2018-07-31 福建省晋江优兰发纸业有限公司 A method of producing high transparency paper with sulphite
CN109810964A (en) * 2017-11-21 2019-05-28 湖南海正生物科技有限公司 A kind of mixing enzyme preparation and preparation method thereof for mashing
CN113355311A (en) * 2020-08-14 2021-09-07 嘉兴景和环保科技有限公司 Biological complex enzyme preparation for softening wood fibers and preparation method thereof
CN112375753A (en) * 2020-11-27 2021-02-19 苏州埃斯腾特生物科技有限公司 Pulping enzyme preparation for electrostatic copying paper and preparation method and application thereof

Similar Documents

Publication Publication Date Title
CN104404017A (en) Complex enzyme preparation and application of complex enzyme preparation to pulping and papermaking
Suurnäkki et al. Trichoderma reesei cellulases and their core domains in the hydrolysis and modification of chemical pulp
Andberg et al. Swollenin from Trichoderma reesei exhibits hydrolytic activity against cellulosic substrates with features of both endoglucanases and cellobiohydrolases
EP2519630B1 (en) Method for treating cellulosic material and cbhii/cel6a enzymes useful therein
ES2527681T3 (en) Treatment of cellulosic material and enzymes useful in it
JP2011515089A5 (en)
Husson et al. Sequential and simultaneous strategies for biorefining of wheat straw using room temperature ionic liquids, xylanases and cellulases
SE533510C2 (en) Method for producing microfibrillar cellulose
Singh et al. Cellulase in pulp and paper industry
CN102888433B (en) Method for improving enzymatic hydrolysis efficiency of bamboo reed stalks
Auxenfans et al. Simultaneous pretreatment and enzymatic saccharification of (ligno) celluloses in aqueous-ionic liquid media: a compromise
CN103255659B (en) The method that ammonia associating diluted alkaline normal pressure processes lignocellulose-like biomass
CN104032379B (en) A kind of preparation method of corn wrapper fibre and the corn wrapper fibre obtained by the method
Faria et al. Production of cellulases and xylanases by Humicola grisea var. thermoidea and application in sugarcane bagasse arabinoxylan hydrolysis
Balsan et al. Characterization of a commercial cellulase for hydrolysis of agroindustrial substrates
CN104342424A (en) Fiber oxidase composition for changing and improving fiber property, and papermaking method and application
Hamisan et al. Delignification of Oil Palm Empty Fruit Bunch using Chemical and
CN103898616A (en) Sisal hemp degumming process
CN102094249B (en) Method for degumming ramie by weakly alkaline liquid biological enzyme
CN104631178A (en) Deep purification method of crude cellulose
Vishwakarma et al. Partial enzymatic cell wall disruption of Oocystis sp. for simultaneous cultivation and extraction
CN107083411A (en) A kind of preprocess method of wheat straw waste enzymolysis
CN108179650A (en) A kind of non-wood fiber wheat straw production technology using black liquor of pulp making production Yellow humic acid fertilizer
CN101168929A (en) Degumming and pulping method for broussonetia papyrifera phloem fiber
Silveira et al. The essential role of plant cell wall degrading enzymes in the success of biorefineries: current status and future challenges

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication

Application publication date: 20150311

RJ01 Rejection of invention patent application after publication