CN104403358A - Preparation method of heatproof anthocyanin pigment - Google Patents
Preparation method of heatproof anthocyanin pigment Download PDFInfo
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- CN104403358A CN104403358A CN201410629320.3A CN201410629320A CN104403358A CN 104403358 A CN104403358 A CN 104403358A CN 201410629320 A CN201410629320 A CN 201410629320A CN 104403358 A CN104403358 A CN 104403358A
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/40—Colouring or decolouring of foods
- A23L5/42—Addition of dyes or pigments, e.g. in combination with optical brighteners
- A23L5/43—Addition of dyes or pigments, e.g. in combination with optical brighteners using naturally occurring organic dyes or pigments, their artificial duplicates or their derivatives
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B67/00—Influencing the physical, e.g. the dyeing or printing properties of dyestuffs without chemical reactions, e.g. by treating with solvents grinding or grinding assistants, coating of pigments or dyes; Process features in the making of dyestuff preparations; Dyestuff preparations of a special physical nature, e.g. tablets, films
- C09B67/0096—Purification; Precipitation; Filtration
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- Life Sciences & Earth Sciences (AREA)
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- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a preparation method of a heatproof anthocyanin pigment. The preparation method comprises the following steps: (1) reflux extraction: using an acidic ethanol solution with a pH value of 2.5 to 3.5 to carry out reflux extraction on anthocyanin plants to obtain an extract; (2) one-step filtration: filtering the extract by a filtering device with an aperture of 150-250 meshes and a filtering device with an aperture of 5 to 15 [mu]m in sequence to obtain a coarse filtrate; (3) reduced pressure condensation: carrying out reduced pressure condensation on the coarse filtrate at a temperature of 40 to 60 DEG C until the baume degree reaches 2 to 4 degrees so as to obtain a condensate; (4) secondary filtration: filtering the obtain condensate by an ultrafiltration membrane to obtain a fine filtrate; (5) thermal treatment: adding at least one of acetic acid, acetic anhydride, humic acid, and sodium humate into the fine filtrate, heating the filtrate for 20 to 40 minutes at a temperature of 65 to 85 DEG C so as to obtain liquid heatproof anthocyanin pigment. The anthocyanin pigment prepared by the provided method can be used to color food, the colored food will not fade, and the color will not change after baking or a thermal treatment.
Description
Technical field
The present invention relates to pigment preparation field, particularly relate to a kind of preparation method with the anthocyani pigment of high heat resistance.
Background technology
Anthocyani pigment is the compounds that anthocyanidin and glycosidic link are combined into, extensively pure be plant flower, really, in the enchylema of stem, leaf and root organ, plant is presented by red, purplish red to the different colours such as blue.Anthocyanogen is flavonoid---can present a red compounds of group in the class material based on flavones core, its color changes with pH value difference, and colour developing is better in acid condition, takes on a red color, in neutral conditions in red-purple, in the basic conditions in blueness.Anthocyanogen has anti-oxidant, scavenging free radicals, minimizing low-density lipoprotein, anti-mutation, antitumor, the effect that improves eyesight.
Along with being showing improvement or progress day by day of science and technology, development grows the food industries of China, and meanwhile, along with the raising of living standards of the people, the health requirements of people to self is also more and more higher, and therefore the safety issue of food and foodstuff additive also becomes focus.Food safety is related to the healthy of broad masses of the people and life security, so the emphasis of concern has been turned to natural additive for foodstuff by people, particularly also has the natural additive for foodstuff of certain pharmacologically active.Thus utilize plant extract natural edible additive in recent years, become the trend that foodstuff additive field is new, be widely used in modulating the food such as breast, jam, candy, cake painted in.But anthocyani pigment is used for after food color, with the rising of food-processing, storage temperature, and the prolongation of time, pigment is degraded, and chroma loss and crest offset, and variable color after causing heating of food, fades.
Therefore, need a kind of be used for food color after, it is variable color and the anthocyani pigment that fades because of heating of food not.
Summary of the invention
For solving the deficiencies in the prior art, the object of the present invention is to provide a kind of preparation method of anthocyani pigment, the anthocyani pigment product obtained by this preparation method has good thermotolerance.
The invention provides a kind of preparation method of heat-resisting anthocyani pigment, comprise the steps:
Refluxing extraction step: by the acid ethanol solution refluxing extraction of anthocyanogen plant material pH2.5-3.5, obtain extracting solution;
A filtration step: the filtration devices by described extracting solution through pore size filter being 150-250 order and 5-15 μm successively, obtains coarse filtration liquid;
Concentrating under reduced pressure step: it is 2-4 ° that described coarse filtration liquid is evaporated to degree Beaume at 40-60 DEG C, obtains concentrated solution;
Secondary filtration step: by described concentrated solution ultrafiltration membrance filter, obtain smart filtrate;
Heat treatment step: add at least one in acetic acid, diacetyl oxide, phytic acid and sodium phytate in described smart filtrate, and heat 20-40 minute at 65-85 DEG C, obtain heat-resisting anthocyani pigment liquid product.
Alternatively, preparation in accordance with the present invention, in described refluxing extraction step, the consumption of described acid ethanol solution is 10-20 times of described anthocyanogen plant material weight, and refluxing extraction carries out 40-60 minute at 40-60 DEG C.
Alternatively, preparation in accordance with the present invention, in described refluxing extraction step, described acid ethanol solution is made up of according to volume ratio 1:1000-10:1000 acid and 15-40vol% ethanol.
Alternatively, preparation in accordance with the present invention, described acid is at least one in hydrochloric acid, sulfuric acid and phosphoric acid.
Alternatively, preparation method of the present invention, in described secondary filtration step, the particle diameter of described ultra-filtration membrane is 0.1-0.2 μm.
Alternatively, preparation in accordance with the present invention, described anthocyanogen plant is raw material red rice, turnip with red inside, purple cabbage or Rhizoma Dioscoreae esculentae.
Alternatively, preparation in accordance with the present invention, comprises pulverising step further: before carrying out refluxing extraction step, and anthocyanogen plant material is crushed to 20-40 order.
Alternatively, preparation method of the present invention, comprises three filtration steps further: by described heat-resisting anthocyanogen liquid product reverse osmosis membrane processing, collects trapped fluid.
Alternatively, preparation in accordance with the present invention, comprises drying step further, and described heat-resisting anthocyanogen liquid product is dried to powdery.
Alternatively, preparation in accordance with the present invention, the polyacrylamide film of described reverse osmosis membrane to be molecular weight cut-off be 500-2000.
The thermotolerance of the anthocyani pigment product obtained by preparation method of the present invention is greater than 96%, and crest skew is less than or equal to 2nm, after this product is used for food color, as cake, candy and jam, through baking and banking up with earth or hot-work, does not have variable color and discolouration phenomena.
Accompanying drawing explanation
By reading hereafter detailed description of the preferred embodiment, various other advantage and benefit will become cheer and bright for those of ordinary skill in the art.Accompanying drawing only for illustrating the object of preferred implementation, and does not think limitation of the present invention.In the accompanying drawings:
Fig. 1 is the schematic flow sheet of heat-resisting anthocyani pigment preparation method of the present invention.
Embodiment
The invention provides many applicable creative concepts, this creative concept can be reflected in a large number of in concrete context.The specific embodiment described in following embodiments of the present invention only as the exemplary illustration of the specific embodiment of the present invention, and does not form limitation of the scope of the invention.
Below in conjunction with accompanying drawing and concrete embodiment, the invention will be further described.
The invention provides a kind of preparation method of heat-resisting anthocyani pigment, Fig. 1 illustrates the schematic flow sheet of preparation method of the present invention.As shown in Figure 1, this comprises the steps:
Refluxing extraction step S1200: by the acid ethanol solution refluxing extraction of anthocyanogen plant material pH2.5-3.5, obtain extracting solution;
A filtration step S1300: the filtration devices by described extracting solution through pore size filter being 150-250 order and 5-15 μm successively, obtains coarse filtration liquid;
Concentrating under reduced pressure step S1400: it is 2-4 ° that described coarse filtration liquid is evaporated to degree Beaume at 40-60 DEG C, obtains concentrated solution;
Secondary filtration step S1500: by described concentrated solution ultrafiltration membrance filter, obtain smart filtrate;
Heat treatment step S1600: add at least one in acetic acid, diacetyl oxide, phytic acid and sodium phytate in described smart filtrate, and heat 20-40 minute at 65-85 DEG C, obtain heat-resisting anthocyani pigment liquid product.
Wherein, in described refluxing extraction step S1200, the consumption of described acid ethanol solution is 10-20 times of described cyanidin(e) plant material weight.Acid ethanol solution is made up of with ethanol acid, generally makes by adding acid in ethanol, constantly stirs and monitor the pH value of mixing solutions, till pH value reaches 2.5-3.5 in the process added.Usually, acid and 15-40vol% ethanol are allocated to make described acid ethanol solution according to the ratio that volume ratio is 1:1000-10:1000.The ethanol of the preferred choice for use 15-40vol% of described ethanol, described acid is at least one in hydrochloric acid, sulfuric acid and phosphoric acid.
In a described filtration step S1300, described filtration unit can be the common filtration units such as such as filter sieve, filter bag, filtering net, screen plate.The pore size filter of described filtration unit is preferably 150-250 order and 5-15 μm successively.Once filter through this, tentatively can remove the macrobead solid matter in extracting solution.
In described concentrating under reduced pressure step S1400, generally pressure is set to 6000-8000Pa, under this condition, concentrates and can reach required degree Beaume in 4-8 hour.The vacuum concentration equipments such as such as vacuum concentration pan can be adopted to concentrate coarse filtration liquid.
In described secondary filtration step S1500, utilize ultra-filtration membrane to purify concentrated solution, isolation and identification, remove granulometric impurity wherein further, improve the quality of final obtained anthocyani pigment product.Preferably, the particle diameter of described ultra-filtration membrane is 0.1-0.2 μm.In the present invention, adopt hollow-fibre membrane as ultra-filtration membrane.Be understandable that, other conventional ultra-filtration membranes are also applicable to the present invention.
In described heat treatment step S1600, the type of heating such as such as water bath with thermostatic control can be adopted to heat smart filtrate, and Heating temperature is preferably 80 DEG C.Wherein, with the weight of described smart filtrate for benchmark, the add-on of described acetic acid, diacetyl oxide, phytic acid and sodium phytate is 0.005-1%.By adding at least one in acetic acid, diacetyl oxide, phytic acid and sodium phytate, assisting the oxonium ion in anthocyanogen to be combined with acyl group, strengthening the stability of anthocyanogen molecular structure, improving the thermotolerance of anthocyanogen.
In the present invention, in order to improve extraction yield, shorten extraction time, before carrying out refluxing extraction step, can also carry out pulverising step S1100, concrete operations are: clean up after anthocyanogen plant material is rejected insect pest and rotten part, and by the raw material pulverizing powdered after cleaning up.Preferably, the particle diameter of described powder is 20-40 order.In the present invention, described anthocyanogen plant material is red rice, turnip with red inside, purple cabbage or Rhizoma Dioscoreae esculentae.
Further, in order to improve the content of active principle in final obtained anthocyani pigment product, preparation method of the present invention also comprises three filtration step S1700, namely, the heat-resisting anthocyanogen liquid product reverse osmosis membrane obtained is carried out purification process, collects trapped fluid.Wherein, the reverse osmosis membrane used in the present invention is preferably the polyacrylamide film that molecular weight cut-off is 500-2000.
Except the anthocyani pigment product of liquid form, the product of powder-form can also be prepared, that is, carry out drying step S1800, the heat-resisting anthocyanogen liquid product obtained is dried to powdery.Usually, drying means adopts spraying dry.Described spray-dired method, for using small-sized spray drier, at inlet temperature 160-180 DEG C, carries out spraying dry under the condition of air outlet temperature 65-80 DEG C.
Below will be described the present invention by embodiment.
Embodiment 1
With 1000 kilograms of red rice for raw material, through cleaning, be crushed to 20 object powder.With the acid ethanol solution (hydrochloric acid and 40vol% ethanol form) of the pH2.5 of 12000 kilograms, refluxing extraction is carried out to the red rice after above-mentioned pulverizing, extract 60 minutes at 40 DEG C, obtain extracting solution.Extracting solution is used successively 150 order filter sieve, 15 μm of filter bags filter, and obtain coarse filtration liquid 10000 kilograms.Concentrating under reduced pressure at 50 DEG C, obtains 4000 kilograms of concentrated solutions.Be that the hollow-fibre membrane of 0.1 μm carries out filtration treatment to the concentrated solution obtained with pore size filter, obtain smart filtrate 3800 kilograms.In described smart filtrate, add 4 kilograms of diacetyl oxides and 2 kilograms of sodium phytates, stir, be placed in 80 DEG C of waters bath with thermostatic control heating 40 minutes, then with molecular weight cut-off be 500 polyacrylamide film the smart filtrate after heating is filtered, must
heat-resisting haematochrome of red rice liquid product 180 kilograms.Obtain spray-dried for described pigment liquid
heat-resisting haematochrome of red rice powder-like product 8.1 kilograms.
Embodiment 2
With 1000 kilograms of turnip with red inside for raw material, through cleaning, be crushed to 40 object powder, with the acid ethanol solution (phosphoric acid and 15vol% ethanol form) of 20000 kilograms of pH3.0, refluxing extraction carried out to the turnip with red inside after above-mentioned pulverizing, extract 50 minutes at 50 DEG C, obtain extracting solution.Extracting solution is used successively 200 order filter sieve, 5 μm of filter bags filter, and obtain coarse filtration liquid 20000 kilograms.Concentrating under reduced pressure at 40 DEG C, obtains 14000 kilograms of concentrated solutions.Be that the hollow-fibre membrane of 0.2 μm carries out filtration treatment to the concentrated solution obtained with pore size filter, obtain smart filtrate 13200 kilograms.In described smart filtrate, add 1 kilogram of acetic acid and 3 kilograms of phytic acid, stir, be placed in 80 DEG C of waters bath with thermostatic control heating 30 minutes, then with molecular weight cut-off be 2000 polyacrylamide film the smart filtrate after heating is filtered, must
heat-resisting radish red pigment liquid product 30 kilograms.Obtain spray-dried for described pigment liquid
heat-resisting radish red pigment powder-like product 3.3 kilograms.
Embodiment 3
With 1000 kilograms of purple cabbage for raw material, through cleaning, be crushed to 40 object powder, with the acid ethanol solution (phosphoric acid and 20vol% ethanol form) of 20000 kilograms of pH3.5, refluxing extraction carried out to the purple cabbage after above-mentioned pulverizing, extract 50 minutes at 50 DEG C, obtain extracting solution.Extracting solution is used successively 200 order filter sieve, 5 μm of filter bags filter, and obtain coarse filtration liquid 20000 kilograms.Concentrating under reduced pressure at 60 DEG C, obtains 12000 kilograms of concentrated solutions.Be that the hollow-fibre membrane of 0.2 μm carries out filtration treatment to the concentrated solution obtained with pore size filter, obtain smart filtrate 11500 kilograms.In described smart filtrate, add 2 kilograms of acetic acid and 2 kilograms of phytic acid, stir, be placed in 80 DEG C of waters bath with thermostatic control heating 30 minutes, then with molecular weight cut-off be 1000 polyacrylamide film the smart filtrate after heating is filtered, must
heat-resisting cabbage extract liquid product 20 kilograms.Obtain spray-dried again for described pigment liquid
heat-resisting cabbage extract powder-like product 2.2 kilograms.
Embodiment 4
With 1000 kilograms of Rhizoma Dioscoreae esculentae for raw material, through cleaning, be crushed to 30 object powder, with the acid ethanol solution (sulfuric acid and 30vol% ethanol form) of 20000 kilograms of pH3.0, refluxing extraction carried out to the Rhizoma Dioscoreae esculentae after above-mentioned pulverizing, extract 40 minutes at 60 DEG C, obtain extracting solution.Extracting solution is used successively 250 order filter sieve, 5 μm of filter bags filter, and obtain coarse filtration liquid 20000 kilograms.Concentrating under reduced pressure at 60 DEG C, obtains 10000 kilograms of concentrated solutions.Be that the hollow-fibre membrane of 0.2 μm carries out filtration treatment to the concentrated solution obtained with pore size filter, obtain smart filtrate 9200 kilograms.In described smart filtrate, add 4 kilograms of phytic acid, stir, be placed in 70 DEG C of waters bath with thermostatic control heating 20 minutes, then with molecular weight cut-off be 1000 polyacrylamide film the smart filtrate after heating is filtered, must
heat-resisting Ipomoea batatas(L.)Lam liquid product 30 kilograms, described pigment liquid is spray-dried again
heat-resisting Ipomoea batatas(L.)Lam powder-like product 3.8 kilograms.
Comparative example 1
With 1000 kilograms of red rice for raw material, through cleaning, the HCl extract with 2% 8 hours, obtains extracting solution.Use 80 order filter bags to extracting liquid filtering, obtain filtrate 10000 kilograms.With the red rice anthocyanogen in absorption with macroporous adsorbent resin filtrate, then use the edible ethanol desorb of 60 °, obtain stripping liquid 400kg.Be 160 kilograms by stripping liquid concentrating under reduced pressure, more spray-dried
common haematochrome of red rice powder-like product 7.2 kilograms.
Comparative example 2
With 1000 kilograms of turnip with red inside for raw material, through cleaning, section, the HCl extract with 3% 12 hours, obtains extracting solution.Use 80 order filter bags to extracting liquid filtering, obtain filtrate 30000 kilograms.With the radish anthocyanogen in absorption with macroporous adsorbent resin filtrate, then use the edible ethanol desorb of 70 °, obtain stripping liquid 400kg.Be 26 kilograms by stripping liquid concentrating under reduced pressure, more spray-dried
common radish red pigment powder-like product 2.9 kilograms.
Comparative example 3
With 1000 kilograms of purple cabbage for raw material, through cleaning, section, the HCl extract with 3% 12 hours, obtains extracting solution.Use 80 order filter bags to extracting liquid filtering, obtain filtrate 30000 kilograms.With the cabbage assortment glycosides in absorption with macroporous adsorbent resin filtrate, then use the edible ethanol desorb of 70 °, obtain stripping liquid 400kg.By stripping liquid concentrating under reduced pressure to 20 kilograms, more spray-dried
common cabbage extracts powdered product 1.8 kilograms.
Comparative example 4
With 1000 kilograms of Rhizoma Dioscoreae esculentae for raw material, through cleaning, section, the HCl extract with 2% 6 hours, obtains extracting solution.Use 80 order filter bags to extracting liquid filtering, obtain filtrate 30000 kilograms.With the anthocyanins pigment from purple sweet potato in absorption with macroporous adsorbent resin filtrate, then use the edible ethanol desorb of 40 °, obtain stripping liquid 400kg.By stripping liquid concentrating under reduced pressure to 25 kilograms, more spray-dried
common Ipomoea batatas(L.)Lam powder-like product 3.4 kilograms.
The anthocyani pigment product prepared by above-described embodiment 1-4 all has good thermotolerance.In order to the thermotolerance of above-mentioned obtained product is described better, the thermotolerance of the heat-resisting anthocyani pigment that applicant is prepared embodiment of the present invention 1-4 by ultraviolet spectrophotometer (UV) and common anthocyani pigment prepared by comparative example 1-4 has carried out comparison and detection.
Detection method: product (being labeled as contrast product 1, contrast product 2, contrast product 3, contrast product 4 respectively) prepared by the product (being labeled as product 1, product 2, product 3, product 4 respectively) prepared by embodiment of the present invention 1-4 and comparative example 1-4, dilute with water is dissolved to mutually homochromy valency
each taking-up 50 grams, puts into 50mL colorimetric cylinder, jam-pack glass stopper, is placed in 80 DEG C of thermostat water baths respectively or 120 DEG C of constant temperature ovens heat, at interval of certain hour, and the feature maximum absorption crest of sampling and measuring pigment and look valency
calculate thermotolerance (storage rate).Concrete detected result is as shown in following table 1-4.
Table 1
Table 2
Table 3
Table 4
As can be seen from above-mentioned table 1-4, the thermotolerance of the anthocyani pigment product obtained by preparation method of the present invention is all greater than 96%, and crest skew is less than or equal to 2nm.This product is used for such as cake, candy and jam food painted after, even if through baking and banking up with earth or hot-work, also there will not be variable color and discolouration phenomena.
More than describe the preferred embodiment of the present invention in detail; but the present invention is not limited to the detail in above-mentioned embodiment, within the scope of technical conceive of the present invention; can carry out multiple simple variant to technical scheme of the present invention, these simple variant all belong to protection scope of the present invention.It should be noted that in addition, each concrete technical characteristic described in above-mentioned embodiment, in reconcilable situation, can be undertaken combining the repetition in order to avoid unnecessary by any suitable mode, the present invention illustrates no longer separately to various possible array mode.
In addition, also can carry out arbitrary combination between various different embodiment of the present invention, as long as it is without prejudice to thought of the present invention, it should be considered as content disclosed in this invention equally.
Claims (10)
1. a preparation method for heat-resisting anthocyani pigment, comprises the steps:
Refluxing extraction step (S1200): by the acid ethanol solution refluxing extraction of anthocyanogen plant material pH2.5-3.5, obtain extracting solution;
A filtration step (S1300): the filtration devices by described extracting solution through pore size filter being 150-250 order and 5-15 μm successively, obtains coarse filtration liquid;
Concentrating under reduced pressure step (S1400): it is 2-4 ° that described coarse filtration liquid is evaporated to degree Beaume at 40-60 DEG C, obtains concentrated solution;
Secondary filtration step (S1500): by described concentrated solution ultrafiltration membrance filter, obtain smart filtrate;
Heat treatment step (S1600): add at least one in acetic acid, diacetyl oxide, phytic acid and sodium phytate in described smart filtrate, and heat 20-40 minute at 65-85 DEG C, obtain heat-resisting anthocyani pigment liquid product.
2. preparation method according to claim 1, wherein, in described refluxing extraction step (S1200), the consumption of described acid ethanol solution is 10-20 times of described anthocyanogen plant material weight, and refluxing extraction carries out 40-60 minute at 40-60 DEG C.
3. preparation method according to claim 1, wherein, in described refluxing extraction step (S1200), described acid ethanol solution is made up of according to volume ratio 1:1000-10:1000 acid and 15-40vol% ethanol.
4. preparation method according to claim 3, wherein, described acid is at least one in hydrochloric acid, sulfuric acid and phosphoric acid.
5. preparation method according to claim 1, wherein, in described secondary filtration step (S1500), the particle diameter of described ultra-filtration membrane is 0.1-0.2 μm.
6. preparation method according to claim 1, wherein, described anthocyanogen plant is raw material red rice, turnip with red inside, purple cabbage or Rhizoma Dioscoreae esculentae.
7. the preparation method according to any one of claim 1-6, wherein, comprises pulverising step (S1100) further: before carrying out refluxing extraction step, anthocyanogen plant material is crushed to 20-40 order.
8. the preparation method according to any one of claim 1-6, wherein, comprises three filtration steps (S1700) further: by described heat-resisting anthocyanogen liquid product reverse osmosis membrane processing, collects trapped fluid.
9. the preparation method according to any one of claim 1-6, wherein, comprises drying step (S1800) further, and described heat-resisting anthocyanogen liquid product is dried to powdery.
10. preparation method according to claim 8, wherein, the polyacrylamide film of described reverse osmosis membrane to be molecular weight cut-off be 500-2000.
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| CN112250724A (en) * | 2020-12-17 | 2021-01-22 | 上海诗丹德标准技术服务有限公司 | Preparation method of reference substance of cornflower-3-coumaroyl-diglucose-5-glucoside |
| CN112250724B (en) * | 2020-12-17 | 2021-03-05 | 上海诗丹德标准技术服务有限公司 | Preparation method of reference substance of cornflower-3-coumaroyl-diglucose-5-glucoside |
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