CN104391089B - Continuous-flow type toxicity test of fishes system - Google Patents

Continuous-flow type toxicity test of fishes system Download PDF

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Publication number
CN104391089B
CN104391089B CN201410746822.4A CN201410746822A CN104391089B CN 104391089 B CN104391089 B CN 104391089B CN 201410746822 A CN201410746822 A CN 201410746822A CN 104391089 B CN104391089 B CN 104391089B
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China
Prior art keywords
toxicity test
fishes
water
flow
mozzle
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Expired - Fee Related
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CN201410746822.4A
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Chinese (zh)
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CN104391089A (en
Inventor
李海山
陈会明
崔媛
沈国林
王琤
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Chinese Academy of Inspection and Quarantine CAIQ
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Chinese Academy of Inspection and Quarantine CAIQ
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Abstract

The invention provides a kind of continuous-flow type toxicity test of fishes system, including Fish temperature environment adjusting means and toxicity test device of fishes;Fish temperature environment adjusting means is the room temp regulator for regulating indoor environment temperature;Toxicity test device of fishes includes: be layered at least one ecological fish bowl of setting the most from low to high, the solution mixer connected one to one by mozzle and ecological fish bowl, the storing medicine bottle connected one to one with solution mixer by mozzle and the water tank being connected with solution mixer by mozzle;The mozzle connecting storing medicine bottle and solution mixer is provided with the first flow controller for regulating drug solution concentration, and the mozzle connecting water tank and solution mixer is provided with the second flow controller for regulating water flow.The present invention, entirely without pump, without directly heating, mechanical frictional heat, decreases the impact on medicine and dissolved oxygen, improves accuracy and the reliability of result of the test.

Description

Continuous-flow type toxicity test of fishes system
Technical field
The present invention relates to testing equipment field, particularly relate to a kind of continuous-flow type toxicity test of fishes system.
Background technology
At present, domestic traditional continuous-flow type toxicity test device of fishes is substantially all and have employed substantial amounts of wriggling Pump, heater and agitator, and these equipment use during, due to mechanical friction, pressure or Directly the mode of heat production produces probabilistic impact to the ingredient in flowing water or dissolved oxygen, thus drops The accuracy of low result of the test and reliability.
Summary of the invention
It is an object of the invention to provide a kind of continuous-flow type toxicity test of fishes system, to solve above-mentioned asking Topic.
The embodiment provides a kind of continuous-flow type toxicity test of fishes system, including: Fish water temperature Environment regulating device and toxicity test device of fishes;Fish temperature environment adjusting means is: for conditioning chamber The room temp regulator of interior ambient temperature;Toxicity test device of fishes includes: the most from low to high At least one ecological fish bowl that layering is arranged, the solution connected one to one by mozzle and ecological fish bowl Blender, the storing medicine bottle connected one to one by mozzle and solution mixer and by mozzle with molten The water tank that liquid mixer connects;The mozzle connecting storing medicine bottle and solution mixer is provided with for adjusting The first flow controller of joint drug solution concentration, connects on the mozzle of water tank and solution mixer It is provided with the second flow controller for regulating water flow.
Further, this system also includes and ecological fish bowl photoenvironment adjusting means one to one, illumination Environment regulating device is arranged at the top of ecological fish bowl.
Further, ecological fish bowl includes that water inlet end and discharge ends, water inlet end and discharge ends are respectively arranged at life Two opposing sidewalls of state fish jar, the vertical height of water inlet end is higher than the vertical height of discharge ends.
Further, three ecological fish bowls form one group, and toxicity test device of fishes includes that least one set is ecological Fish jar, often in group, an ecological fish bowl is used for carrying out testing without medicine blank, two other ecological fish Cylinder is for carrying out the experiment of adding consistency gradient group.
Further, first flow controller and second flow controller are quiet flow controller.
Further, this system also includes that the water inlet for resource of water supply carries out sterilizing and filtration treatment processes Device.
Further, water inflow disposal plant includes UV processor and the first filter.
Further, this system also includes for the waste water discharged carries out the useless of ion exchange and filtration treatment Water treatment facilities.
Further, wastewater treatment equipment includes ion exchanger and the second filter.
Further, the system frame for supporting this toxicity test of fishes system is also included.
Compared with prior art the invention has the beneficial effects as follows: by room temp regulator in ecological fish bowl Water temperature be adjusted, toxicity test device of fishes uses the design of vertical demixing formula, liquid flowing completely with Natural landing modes is carried out, entirely without pump, without directly heating, therefore mechanical frictional heat, from And decrease the impact on medicine and dissolved oxygen, improve accuracy and the reliability of result of the test.
Accompanying drawing explanation
Fig. 1 is the structural representation of continuous-flow type toxicity test of fishes system of the present invention;
Fig. 2 is the three-dimensional structure diagram of continuous-flow type toxicity test of fishes system ecology fish jar of the present invention;
Fig. 3 is the plane rip cutting figure of continuous-flow type toxicity test of fishes system ecology fish jar of the present invention.
Detailed description of the invention
Below by specific embodiment and combine accompanying drawing the present invention is described in further detail.
Shown in ginseng Fig. 1 to Fig. 3, Fig. 1 is the structural representation of continuous-flow type toxicity test of fishes system of the present invention Figure;Fig. 2 is the three-dimensional structure diagram of continuous-flow type toxicity test of fishes system ecology fish jar of the present invention;Fig. 3 is The plane rip cutting figure of continuous-flow type toxicity test of fishes system ecology fish jar of the present invention.
Present embodiments provide a kind of continuous-flow type toxicity test of fishes system, including: Fish temperature environment is adjusted Regulating device and toxicity test device of fishes;Fish temperature environment adjusting means is: be used for regulating indoor environment The room temp regulator of temperature;Toxicity test device of fishes includes: layering the most from low to high sets At least one ecological fish bowl 1 put, the solution connected one to one with ecological fish bowl 1 by mozzle 2 Blender 3, the storing medicine bottle 4 connected one to one by mozzle 2 and solution mixer 3 and by leading The water tank 5 that flow tube 2 is connected with solution mixer 3;Connect storing medicine bottle 4 and solution mixer 3 Mozzle 2 is provided with the first flow controller 6 for regulating drug solution concentration, connects water tank 5 and the mozzle 2 of solution mixer 3 be provided with the second flow controller 7 for regulating water flow.
The toxicity test of fishes system that the present embodiment provides, by room temp regulator in ecological fish bowl 1 Water temperature be adjusted, toxicity test device of fishes uses the design of vertical demixing formula, liquid flowing completely with Natural landing modes is carried out, and is accurately regulated by first flow controller 6 and second flow controller 7 The flow that in control system, flowing water carries with drug solution, flowing water and drug solution are decided at the higher level but not officially announced at solution mixer Ratio flows into ecological fish bowl naturally after dilution mixture, entirely without pump, without directly heating, and therefore mechanical Frictional heat, thus decrease the impact on medicine and dissolved oxygen, improve result of the test accuracy and Reliability, meanwhile, whole system does not use peristaltic pump, heater and agitator, has reached energy-conservation, fall The effect of power consumption.
Ecological fish bowl 1 in the present embodiment can use 5L volume, acrylic material, has overflow-type and sets The fish jar of meter, fish jar has water inlet end 9 and discharge ends 10, water inlet end 9 and discharge ends 10 and is respectively provided with In two opposing sidewalls of ecological fish bowl 1, the vertical height of water inlet end 9 is vertical higher than discharge ends 10 Highly, flowing water can form flowable state in cylinder, enters draining convenient and strength-saving.Wherein, three ecological fish bowls 1 Forming one group, toxicity test device of fishes includes least one set ecological fish bowl, often in group, and an ecological fish Cylinder 1 is used for carrying out testing without medicine blank, and two other ecological fish bowl 1 is used for carrying out adding consistency ladder The experiment of degree group.The quantity of ecological fish bowl 1 can increase according to the demand of experimental group, but needs in groups Increasing, the fish jar number i.e. increased should be the multiple of 3.
In the present embodiment, the mozzle 2 for supplying water can use PAP material, for for medicinal liquid Mozzle 2 uses the tubing of acid and alkali-resistance, corrosion.
Solution mixer 3 in the present embodiment can use 800ml, glass material, funnel, distance leakage With the container of gap at bucket top edge 50mm.
Storing medicine bottle 4 in the present embodiment can use the brown mold of thread mouth glass bottle of 5000ml, with PP spiral shell Spiral cover and PP topple over ring.
Water tank 5 in the present embodiment can use orthobaric volume to be 40L, and face intersection is 90 degree of radians Angle, the casing using high-quality 304 stainless steel material to manufacture, it is furnished with liquid level controller in case, there is low water Position automatic water supplement and high water level are automatically switched off the function of water source switch.
First flow controller 6 in the present embodiment and second flow controller 7 can use 0~ 2000ml/min, can realize the flow that thinner ratio is 1:3~200 (flow-rate ratio) of drug solution and water inlet Controller.The water flow range of accommodation of second flow controller 7 may be configured as 1~2000ml/min, the The flow adjustment range of the drug solution of one flow controller 6 may be configured as 1~2000ml/min.
Room temp regulator in the present embodiment can use Laboratory Air-conditioning System to carry out environment temperature control regulation, Control ambient temperature, at 5~35 DEG C, provides stable temperature for test Fish.
In the present embodiment, this system can also arrange and regulate with ecological fish bowl photoenvironment one to one Device 8, photoenvironment adjusting means 8 is arranged at the top of ecological fish bowl 1, is used for controlling intensity of illumination, Illumination brightness is adjustable, and switch can regularly, and the illumination variation round the clock in simulating natural environment makes test Fish Live in and be more nearly in required true water environment.Photoenvironment adjusting means 8, every three one Group, often organizes corresponding one group of ecological fish bowl, it is ensured that often the ecological fish bowl illumination in group is identical.Each illumination ring The illumination brightness regulation scope of border adjusting means 8 is 0~18000LX,
In the present embodiment, first flow controller 6 and second flow controller 7 are quiet flow-control Device, by using quiet flow controller, adds native system and does not use peristaltic pump, heating rod, stirring The equipment that the noises such as device are bigger, can accomplish super-silent effect, will not worry that noise is to Fish and tested material Matter produces any impact, it is ensured that the reliability of test, is a kind of energy-conservation, toxicity in fish examination of low noise Check system.
Embodiments of the invention are in order to solve traditional experiment continuous-flow type toxicity test device of fishes, and many employings are certainly Water is direct resource of water supply, it is impossible to the stability at guarantee test water source and reliability, giving up after test Water is directly discharged in environment, environment causes the technical problem of pollution, is configured with use within the system In resource of water supply being carried out sterilizing and the water inflow disposal plant of filtration treatment and for the waste water discharged Carry out the wastewater treatment equipment of ion exchange and filtration treatment.
In the present embodiment, this water inflow disposal plant can use UV processor 11 and the first filter 12. UV processor 11 and the first filter 12 are combined into water inlet treatment system, and feed source is entered by the direction of arrow First filter 12 and UV processor 11.Water inlet water source typically requires as tap water or laboratory central authorities Pure water, PH:6.5~8.5, electrical conductivity: 125~1000 μ s/cm, flow of inlet water >=30L/h.Water in system Source water quality requirement PH=6.5~8.5, electrical conductivity 125~1000 μ s/cm.First filter 12 can use 0.45 μm aperture filter membrane, main filtration falls into the coarseparticulate in water and the material of easy fouling, protection examination Check system pipeline.Select the UV processor 11 of 254nm wave-length coverage, kill harmful microorganism in water, Protecting ecology fish jar is tested Fish.By configuring water inflow disposal plant, not only ensure that test water source Stability and reliability, and ensure that the standardization of ecological fish bowl Mesichthyes current.
In the present embodiment, this wastewater treatment equipment can use ion exchanger 13 and the second filter 14.Second filter 14 is for removing the particulate matter in waste water, and ion exchanger 13 is after to test Waste water carry out ion-exchange treatment, remove ionic compound harmful in waste water, through the second filter 14 And ion exchanger 13 process after waste water discharged by the direction of arrow.By configuring wastewater treatment equipment, The waste water that the system that ensure that is discharged at waste water discharge opening does not produce any pollution to environment.
In the present embodiment, also include the system frame 15 for supporting this toxicity test of fishes system, be System support 15 uses 304 stainless steels, sturdy and durable, has subregion, layering, function of supplying power, Level is to being divided into two-layer, vertically to being divided into three layers.Wherein level is to being divided into trial zone and two, water treatment zone Functional areas.Vertically to being respectively as follows: top layer distribution water tank 5 and the storing medicine bottle 4 of drug solution, middle level Distribution solution mixer 3 and flow controller (include first flow controller 6, second flow controller 7), bottom distribution ecological fish bowl 1 and photoenvironment adjusting means 8.By subregion, hierarchical design not only Realize the powerful support to whole system, and be easy to management, convenient test, improve test efficiency, Frame system 15 provides corresponding power supply in each functional areas, is not furnished with main power.Power requirement: power supply Scope: AC 220V (± 10%), 50Hz.
The mounting condition of the present embodiment pilot system: not less than 20m2Independent room, can cover light source, There is satisfactory water inlet water source, drainage pipeline, constant temperature and humidity air-conditioning system (temperature controlling range: 18~30 ± 1 DEG C, humid control scope: 45~70 ± 5%).
The pilot system of the present embodiment, it is possible to the dynamic flowing water Mesichthyes growth under true simulating natural condition Environment, launch chemicals toxicity in fish evaluation test research, the assessment of the bioaccumulation ability of chemicals and Identify the research of environmental hormone (incretion interferent);The research of residual pesticide in environment;Specific drugs Management and the test of new drug curative effect of medication;The environmental experiment research of breeding;Nutrient test experiments etc..
The a series of detailed description of those listed above is only for the feasibility embodiment of the present invention Illustrate, they also are not used to limit the scope of the invention, all without departing from skill of the present invention essence Equivalent implementations or change that god is made should be included within the scope of the present invention.
It is obvious to a person skilled in the art that the invention is not restricted to the thin of above-mentioned one exemplary embodiment Joint, and without departing from the spirit or essential characteristics of the present invention, it is possible to other concrete shape Formula realizes the present invention.Therefore, no matter from the point of view of which point, embodiment all should be regarded as exemplary, And be nonrestrictive, the scope of the present invention is limited by claims rather than described above, because of This is intended to include at this all changes fallen in the implication of equivalency and scope of claim In bright.Should not be considered as limiting involved claim by any reference in claim.
Moreover, it will be appreciated that although this specification is been described by according to embodiment, but the most each Embodiment only comprises an independent technical scheme, and this narrating mode of description is only is clear Chu Qijian, those skilled in the art should be using description as an entirety, the technology in each embodiment Scheme can also form, through appropriately combined, other embodiments that it will be appreciated by those skilled in the art that.

Claims (5)

1. a continuous-flow type toxicity test of fishes system, it is characterised in that including: Fish temperature environment Adjusting means and toxicity test device of fishes;Described Fish temperature environment adjusting means is: for conditioning chamber The room temp regulator of interior ambient temperature;Described toxicity test device of fishes includes: vertically by low At least one ecological fish bowl arranged to high stratification, by mozzle with described ecological fish bowl one_to_one corresponding even The solution mixer connect, the storing medicine bottle connected one to one with described solution mixer by mozzle and logical Cross the water tank that mozzle is connected with described solution mixer;Connect described storing medicine bottle and described solution mixes The mozzle of clutch is provided with the first flow controller for regulating drug solution concentration, connects described confession The mozzle of water tank and described solution mixer is provided with the second flow control for regulating water flow Device processed;Described first flow controller and second flow controller are by flowing water and medicine in regulation and control system The flow of thing solution conveying, makes flowing water and drug solution after definite proportion dilution mixture in described solution mixer Naturally described ecological fish bowl is flowed into;Described first flow controller and described second flow controller are quiet Flow controller;
Described continuous-flow type toxicity test of fishes system also includes and the illumination one to one of described ecological fish bowl Environment regulating device, described photoenvironment adjusting means is arranged at the top of described ecological fish bowl;
Described continuous-flow type toxicity test of fishes system also includes for resource of water supply is carried out sterilizing and filtration The water inflow disposal plant that processes and for the waste water discharged being carried out the useless of ion exchange and filtration treatment Water treatment facilities;
Described continuous-flow type toxicity test of fishes system also includes for supporting described toxicity test of fishes system System frame;Described system frame in level to being divided into two-layer, vertically to being divided into three layers;Wherein, water Putting down to being divided into trial zone and water treatment zone, described toxicity test device of fishes and photoenvironment are arranged in trial zone Adjusting means, described water inflow disposal plant and wastewater treatment equipment are arranged in water treatment zone;Vertical to being respectively as follows: Top layer arranges described water tank and storing medicine bottle, and middle level arranges that described solution mixer, first flow control Device and second flow controller, bottom arranges described ecological fish bowl and photoenvironment adjusting means.
Continuous-flow type toxicity test of fishes system the most according to claim 1, it is characterised in that institute State ecological fish bowl and include that water inlet end and discharge ends, described water inlet end and discharge ends are respectively arranged at described ecology Two opposing sidewalls of fish jar, the vertical height of described water inlet end is higher than the vertical height of described discharge ends.
Continuous-flow type toxicity test of fishes system the most according to claim 2, it is characterised in that three Individual described ecological fish bowl forms one group, and described toxicity test device of fishes includes ecological fish described in least one set Cylinder, often in group, described ecological fish bowl is used for carrying out testing without medicine blank, described in two other Ecological fish bowl is for carrying out the experiment of adding consistency gradient group.
Continuous-flow type toxicity test of fishes system the most according to claim 1, it is characterised in that institute State water inflow disposal plant and include UV processor and the first filter.
Continuous-flow type toxicity test of fishes system the most according to claim 1, it is characterised in that institute State wastewater treatment equipment and include ion exchanger and the second filter.
CN201410746822.4A 2014-12-08 2014-12-08 Continuous-flow type toxicity test of fishes system Expired - Fee Related CN104391089B (en)

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CN106153880A (en) * 2016-07-20 2016-11-23 苏州华测安评化学品技术服务有限公司 Fish continuous-flow type test system
CN106442959B (en) * 2016-10-10 2018-09-07 环境保护部南京环境科学研究所 Daphnia magna continuous-flow type toxicity test device
CN109254132B (en) * 2018-10-12 2023-11-21 中国环境科学研究院 Microcosm experimental device for simulating pond ecosystem
CN109254131A (en) * 2018-10-12 2019-01-22 中国环境科学研究院 A kind of indoor microcosm imitative experimental appliance for simulating river ecosystem
CN110208483B (en) * 2019-07-11 2021-09-07 上海海洋大学 Method for testing ecological toxicity of marine crustacean water
CN112268998A (en) * 2020-10-16 2021-01-26 西咸新区国睿一诺药物安全评价研究有限公司 Running water type system applied to fish toxicity test

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JPH08214726A (en) * 1995-02-08 1996-08-27 Meito Suien:Kk Water tank unit for rearing fish and shellfish
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