CN104387487A - Preparation method of sulfated water-soluble apple dietary fiber based on aqueous medium - Google Patents
Preparation method of sulfated water-soluble apple dietary fiber based on aqueous medium Download PDFInfo
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Abstract
The invention discloses a preparation method of sulfated water-soluble apple dietary fiber based on an aqueous medium. The preparation method comprises the following steps: 1) modifying the apple dietary fiber by extruding by use of an extrusion press, thereby obtaining the extrusion-modified apple dietary fiber; 2) extracting the water-soluble apple dietary fiber by use of a water extraction and alcohol precipitation method; 3) by taking sulfamic acid-concentrated sulfuric acid as a composite esterifying agent for sulfating and taking water as a reaction medium, performing ultrasonic-assisted sulfating on the water-soluble apple dietary fiber to obtain the sulfated water-soluble apple dietary fiber having higher physiological activity. As the water is taken as the reaction medium, the prepared sulfated water-soluble apple dietary fiber has no organic solvent residual, and therefore, the preparation method of the sulfated water-soluble apple dietary fiber based on the aqueous medium is a safe and green sulfating method, and is simple and feasible, environment-friendly and suitable for industrial large-scale production.
Description
Technical field
The invention belongs to food processing technology field, be specifically related to the sulphating method of modifying of the apple dietary fiber that a kind of green remains without organic reagent.
Background technology
China is apple production big country, and fruit juice can produce a large amount of apple residues in the course of processing.It is reported that food fibre is up to 60 ~ 80% in apple residue, is the very good material preparing food fibre.Food fibre, as the important composition composition of food, is called as " the seventh-largest nutrient substance ", has the different physiological roles such as suitable intestines defaecation, regulable control blood sugar concentration, reducing blood-fat.By solvability, food fibre can be divided into water-soluble dietary fibre (SDF) and water insoluble dietary fiber (IDF).Wherein SDF has the physiologically active stronger than IDF in prevent diabetes, cardiovascular disorder, reduction cholesterol etc.
From structure, SDF is the mixed polysaccharide be polymerized by semi-lactosi, glucose, wood sugar etc., and vegetable polysaccharides solubleness is low, himself does not possess that some is active or biological activity is very weak.In recent years, investigator finds that sulphating is a kind of effective polyose modification method, the hydroxyl on polysaccharide macro-molecular monosaccharide molecule replace by sulfate radical, there is anticoagulation, the physiologically active such as antitumor, antiviral.The method of Sulfate of polysaccharide is a lot, and according to the difference of sulfur acidizing reagent used, conventional method has: sulphate method, sulphur trioxide method, chlorosulfonic acid method.Polysaccharide sulfated research shows, the sulphoating agent vitriol oil, sulphur trioxide, chlorsulfonic acid etc. are used alone and can cause polysaccharide severely degrade, and Lewis base medium can reduce DeR, and therefore esterified solvent effect is in the esterification reaction very crucial.Esterification medium conventional in sulfuric acid esterification has methane amide, pyridine, dimethyl formamide (DMF).
Publication number is that the Chinese patent of CN1651466A discloses a kind of sulphating derivative of polysaccharide and method for making thereof and purposes, this patent adopts chlorsulfonic acid pyridine method to carry out sulphating modification to marine fungi polysaccharide YCP, and obtained controlling sulfate polyose substitution value is 0.13 ~ 1.3; Publication number is the preparation method that the Chinese patent of CN102219865A discloses the cherokee rose polysaccharide derivatives with anti-tumor activity, this patent adopts chlorsulfonic acid-DMF legal system for Fructus Rosae Laevigatae polysaccharides sulphating derivative, and it is inhibited to the propagation of rectum cancer cell; Publication number is the preparation method that the Chinese patent of CN1786034A discloses umbellate pore fungus polysaccharide sulphate, adopts chlorsulfonic acid-methane amide method sulphating polyporusum bellatus, obtains the umbellate pore fungus polysaccharide sulphate that biological activity is higher.Above-mentioned esterification medium is volatile organic solvent, and the controlling sulfate polyose of preparation exists the problems such as organic solvent residual, produces potential safety hazard, makes it be restricted in the application aspect of food, medicine.
Huang Xue's virtue waits (orthogonal optimization Arillus longan polysaccharide sulfuric acid esterification condition research, contemporary Chinese medicinal application, 2011,5,124-125) adopt sulphate method to carry out sulphating modification to longan polysaccharide, the sulphating longan polysaccharide substitution value of acquisition is 2.03, but vitriol oil esterification needs to react under cryogenic, otherwise the carboniogenesis due to the vitriol oil makes coloured product comparatively dark, and operational condition is harsh; Publication number is the method for modifying that the Chinese patent of CN103610081A discloses a kind of apple dietary fiber, this patent adopts thionamic acid-pyridine method to carry out sulphating modification to apple dietary fiber, the sulphating apple dietary fiber substitution value of preparation is 1.29, but the method carries out esterification in pyridine medium, pyridine medium toxicity is large, volatile, smell is pungent.
Summary of the invention
The object of the present invention is to provide a kind of preparation method of the sulphating apple water-soluble dietary fibre based on water medium, the apple water-soluble dietary fibre that there is not organic solvent residual, there is high physiologically active can be prepared.
For achieving the above object, present invention employs following technical scheme:
1) distilled water of apple dietary fiber and apple dietary fiber weight 20 ~ 40% is uniformly mixed rear standing 30 ~ 120min, extrusion modification is carried out, then drying, apple dietary fiber extrusion modification thing (i.e. extrusion modification apple dietary fiber) of pulverizing and sieve to obtain successively after leaving standstill;
2) add in apple dietary fiber extrusion modification thing after distilled water in 50 ~ 70 DEG C of lixiviate 1 ~ 2h, the mass ratio of apple dietary fiber extrusion modification thing and distilled water is 1:20 ~ 1:40, lixiviate terminates rear suction filtration and collects filtrate, 95% ethanol of 1 ~ 4 times of volume is added and hold over night (water extraction and alcohol precipitation method) after being concentrated by filter vacuum, then centrifugal and collecting precipitation, dries to obtain apple water-soluble dietary fibre by precipitation;
3) apple water-soluble dietary fibre is dissolved in distilled water and obtains mixture A, in mixture A, add thionamic acid under agitation and drip the vitriol oil, then be 20 ~ 40kHz in ultrasonic frequency, temperature is react 1 ~ 4h under the condition of 60 ~ 90 DEG C, room temperature is cooled to after reaction terminates, then be neutral by 2 ~ 4mol/LNaOH aqueous solution adjust ph, then 95% ethanol of 1 ~ 4 times of volume is added and hold over night, then centrifugal and collecting precipitation, precipitation is dissolved in distilled water and loads in dialysis tubing, then distill water dialysis is used 2 ~ 4 days, then to (referring to the liquid in dialysis tubing) in dialyzate and add 95% ethanol of 1 ~ 4 times of volume and hold over night, then centrifugal and collecting precipitation, precipitation is dried to obtain sulphating apple water-soluble dietary fibre (called after SSDF).
Described extrusion modification is extruding puffing under screw extruder screw speed is 700 ~ 900r/min condition.
The condition of described vacuum concentration is: temperature is 45 ~ 60 DEG C, and vacuum tightness is 0.06 ~ 0.09MPa, is concentrated into 1/4 ~ 1/6 of filtrate volume.
In described mixture A, the mass ratio of apple water-soluble dietary fibre and distilled water is 1:50 ~ 1:80.
The mass ratio of described apple water-soluble dietary fibre and complex ester agent is 1:1 ~ 1:7, and complex ester agent is described thionamic acid and the vitriol oil.
The mass ratio of described thionamic acid and the vitriol oil is 1:0.5 ~ 1:4.
The preparation method of described apple dietary fiber comprises the following steps: apple residue and distilled water are uniformly mixed rear use 2 ~ 4mol/L NaOH aqueous solution and regulate pH to be 10 ~ 14, the mass ratio of apple residue and distilled water is 1:6 ~ 1:10, then mixture B is obtained in 50 ~ 80 DEG C of insulation 10min, the hydrogen peroxide of distilled water volume 1 ~ 3% is added to mixture B, then in 50 ~ 80 DEG C of stirring reaction 1 ~ 3h, stirring reaction terminates rear water displacement wash to neutral, after washing successively drying, pulverize and sieve, obtain apple dietary fiber.
The temperature of described oven dry is 35 ~ 60 DEG C; Described sieving all adopts 20 ~ 80 mesh sieves.
Compared with prior art, the present invention has following useful technique effect:
(1) reaction medium that the present invention adopts is water, has the advantages such as nontoxic, colourless, tasteless, not volatile.Obtained product safety, organic solvent-free remain, and compare conventional reaction medium pyridine, methane amide etc. safer.
(2) the present invention adopts ultrasonic assistant apple SDF sulphating, shorten esterification time, reduce apple SDF to degrade under sour environment, improve reaction efficiency, obtained sulphating apple SDF is of light color, and yield is high, and esterification technique is simple to operate, environmental friendliness, is applicable to industrialization scale operation.
(3) the present invention's complex ester agent that is sulphating with thionamic acid-vitriol oil, enzymatic synthesis condition is easy to control, and so both avoiding vitriol oil esterification needs to build cold condition, solves again the low problem of thionamic acid reactive behavior in water medium.
(4) the sulphating apple SDF that the present invention prepares has significant restraining effect to breast cancer cell MM231 propagation.
(5) the present invention is by processing apple residue, not only play decolorization, also improve the content of apple dietary fiber in process product, by carrying out extrusion modification process to apple dietary fiber further, not only significantly improve SDF composition, improve SDF retention ability, bulging force etc. simultaneously.
Embodiment
Elaborate to the present invention below in conjunction with embodiment, the explanation of the invention is not limited.
The preparation method of apple dietary fiber: take certain mass 20 order apple residue, the ratio being 1:8 (g:mL) according to solid-liquid ratio adds distilled water, stir, regulate pH to be 13 with 2mol/L aqueous sodium hydroxide solution, in 70 DEG C of insulation 10min, then the hydrogen peroxide of distilled water volume 2% is added, 70 DEG C of stirring reaction 2h, question response terminates rear mistake 100 mesh sieve, and screen overflow is extremely neutral with water displacement wash, be placed on oven dry in 55 DEG C of baking ovens, pulverize and cross 20 mesh sieves, obtain apple dietary fiber.
Embodiment 1
1) certain mass 20 order apple dietary fiber is taken, add the distilled water of apple dietary fiber quality 20%, stir, after leaving standstill 30min, extruding puffing under screw speed is 700r/min condition, after extrusion process, extrudate is placed in 45 DEG C of baking ovens to dry, pulverizes and cross 20 mesh sieves, obtain extrusion modification apple dietary fiber;
2) certain mass 20 order extrusion modification apple dietary fiber is taken, distilled water is added by the solid-liquid ratio (g:mL) of 1:20, in 50 DEG C of lixiviate 2h, suction filtration also collects filtrate, and filtrate is 60 DEG C in temperature, vacuum tightness is vacuum concentration to 1/5 volume under 0.07MPa, add 4 times of volume 95% ethanol alcohol precipitations, hold over night, centrifugal and collecting precipitation, precipitation is placed in 45 DEG C of baking ovens to dry, obtains apple SDF;
3) 1.0g apple SDF is accurately taken, be dissolved in 50mL water, add 1.0g thionamic acid under agitation and slowly drip the 2mL vitriol oil, subsequently in ultrasonic frequency be 20kHz, temperature is react 4h at 60 DEG C, room temperature is cooled to after reaction terminates, be neutral by 2.5mol/L aqueous sodium hydroxide solution adjust ph subsequently, add 4 times of volume 95% ethanol alcohol precipitations, hold over night, centrifugal collecting precipitation, precipitation is dissolved in distilled water and loads in dialysis tubing, distill water dialysis 3 days, dialyzate adds 4 times of volume 95% ethanol alcohol precipitations, hold over night, centrifugal and collecting precipitation, precipitation is placed in 45 DEG C of baking ovens to dry, namely the SSDF of 0.564g is obtained.
Embodiment 2
1) certain mass 20 order apple dietary fiber is taken, add the distilled water of apple dietary fiber quality 30%, stir, after leaving standstill 60min, extruding puffing under screw speed is 800r/min condition, after extrusion process, extrudate is placed in 50 DEG C of baking ovens to dry, pulverizes and cross 40 mesh sieves, obtain extrusion modification apple dietary fiber;
2) certain mass 40 order extrusion modification apple dietary fiber is taken, distilled water is added by the solid-liquid ratio (g:mL) of 1:30, in 60 DEG C of lixiviate 1.5h, suction filtration also collects filtrate, and filtrate is 50 DEG C in temperature, vacuum tightness is vacuum concentration to 1/5 volume under 0.06MPa, add 4 times of volume 95% ethanol alcohol precipitations, hold over night, centrifugal and collecting precipitation, precipitation is placed in 50 DEG C of baking ovens to dry, obtains apple SDF;
3) 1.0g apple SDF is accurately taken, be dissolved in 80mL water, add 1.0g thionamic acid under agitation and slowly drip the 1mL vitriol oil, subsequently in ultrasonic frequency be 30kHz, temperature is react 1h at 90 DEG C, be neutral by 2.5mol/L aqueous sodium hydroxide solution adjust ph subsequently, add 4 times of volume 95% ethanol alcohol precipitations, hold over night, centrifugal collecting precipitation, precipitation is dissolved in distilled water and loads in dialysis tubing, distill water dialysis 3 days, dialyzate adds 4 times of volume 95% ethanol alcohol precipitations, hold over night, centrifugal and collecting precipitation, precipitation is placed in 50 DEG C of baking ovens to dry, namely the SSDF of 0.568g is obtained.
Embodiment 3
1) certain mass 20 order apple dietary fiber is taken, add the distilled water of apple dietary fiber quality 40%, stir, after leaving standstill 90min, extruding puffing under screw speed is 900r/min condition, after extrusion process, extrudate is placed in 55 DEG C of baking ovens to dry, pulverizes and cross 60 mesh sieves, obtain extrusion modification apple dietary fiber;
2) certain mass 60 order extrusion modification apple dietary fiber is taken, distilled water is added by the solid-liquid ratio (g:mL) of 1:40, in 70 DEG C of lixiviate 1h, suction filtration also collects filtrate, and filtrate is 55 DEG C in temperature, vacuum tightness is vacuum concentration to 1/6 volume under 0.08MPa, add 4 times of volume 95% ethanol alcohol precipitations, hold over night, centrifugal and collecting precipitation, precipitation is placed in 55 DEG C of baking ovens to dry, obtains apple SDF;
3) 1.0g apple SDF is accurately taken, be dissolved in 60mL water, add 2.0g thionamic acid under agitation and slowly drip the 2mL vitriol oil, subsequently in ultrasonic frequency be 40kHz, temperature is react 3h at 70 DEG C, room temperature is cooled to after reaction terminates, be neutral by 2.5mol/L aqueous sodium hydroxide solution adjust ph subsequently, add 4 times of volume 95% ethanol alcohol precipitations, hold over night, centrifugal collecting precipitation, precipitation is dissolved in distilled water and loads in dialysis tubing, distill water dialysis 3 days, dialyzate adds 4 times of volume 95% ethanol alcohol precipitations, hold over night, centrifugal and collecting precipitation, precipitation is placed in 55 DEG C of baking ovens to dry, namely the SSDF of 0.648g is obtained.
Embodiment 4
1) certain mass 20 order apple dietary fiber is taken, add the distilled water of apple dietary fiber quality 20%, stir, after leaving standstill 120min, extruding puffing under screw speed is 825r/min condition, after extrusion process, extrudate is placed in 60 DEG C of baking ovens to dry, pulverizes and cross 80 mesh sieves, obtain extrusion modification apple dietary fiber;
2) certain mass 80 order extrusion modification apple dietary fiber is taken, distilled water is added by the solid-liquid ratio (g:mL) of 1:25, in 60 DEG C of lixiviate 2h, suction filtration also collects filtrate, and filtrate is 45 DEG C in temperature, vacuum tightness is vacuum concentration to 1/5 volume under 0.09MPa, add 4 times of volume 95% ethanol alcohol precipitations, hold over night, centrifugal and collecting precipitation, precipitation is placed in 60 DEG C of baking ovens to dry, obtains apple SDF;
3) 1.0g apple SDF is accurately taken, be dissolved in 80mL water, add 2.0g thionamic acid under agitation and slowly drip the 1mL vitriol oil, subsequently in ultrasonic frequency be 30kHz, temperature is react 2h at 80 DEG C, room temperature is cooled to after reaction terminates, be neutral by 2.5mol/L aqueous sodium hydroxide solution adjust ph subsequently, add 4 times of volume 95% ethanol alcohol precipitations, hold over night, centrifugal collecting precipitation, precipitation is dissolved in distilled water and loads in dialysis tubing, distill water dialysis 3 days, dialyzate adds 4 times of volume 95% ethanol alcohol precipitations, hold over night, centrifugal and collecting precipitation, precipitation is placed in 60 DEG C of baking ovens to dry, namely the SSDF of 0.562g is obtained.
First the present invention prepares apple dietary fiber under alkaline environment; Secondly carry out extrusion modification process to apple dietary fiber, after extrusion modification process, water soluble dietary fiber content is increased to 23.5% from 3.4% raw material; Water extraction and alcohol precipitation method is adopted to extract water-soluble dietary fibre subsequently; Finally with thionamic acid and the vitriol oil (98% vitriol oil, density 1.84g/mL), for esterifying agent, water makes reaction medium, carries out sulphating modification, obtain the apple dietary fiber of high biological activity to gained water-soluble dietary fibre.
The present invention determines the increment inhibition of sulphating apple SDF to breast cancer cell MM231: under same concentrations, sulphating apple SDF to the restraining effect of MM231 cell apparently higher than apple SDF.When concentration is 20mg/L, the most remarkable to the restraining effect of MM231 cell, its inhibiting rate difference 58.42%, 31.79%.Utilize Graphpad computed in software IC50, obtain apple SDF and the IC50 of sulphating apple SDF to MM231 cell 48h is respectively 268.3mg/L, 16.27mg/L.As can be seen here, water medium sulphating apple SDF can significantly improve the restraining effect of apple SDF to MM231 cell proliferation.
For the defect that existing sulphating method exists, the complex ester agent of the present invention using thionamic acid-vitriol oil as sulphating, take water as reaction medium, with ultrasonic assistant apple SDF sulphating, obtain the sulphating apple SDF with higher physiologically active.Advantage of the present invention is: 1) select thionamic acid-vitriol oil as the complex ester agent of sulphating, avoid thionamic acid and be used alone the low deficiency of esterification activity in water medium, this is because polysaccharide esterification belongs to reversible reaction, the unfavorable controlling sulfate polyose preparing high substitution value of water medium, and the vitriol oil has strongly-acid and strong protonated ability, promote the generation of alkylsulfonyl (-SO3H), reduce reaction activity, improve speed of reaction, be used as the catalyzer of esterification, therefore the present invention selects thionamic acid-vitriol oil as the complex ester agent of sulphating, so both avoid vitriol oil esterification to need to build cold condition, solve the problems such as thionamic acid reactive behavior in water medium is low again.2) adopt ultrasonic assistant apple SDF sulphating, utilize ultrasonic technique to make apple SDF and thionamic acid be dispersed in water on the one hand, the contact of strengthening apple SDF and esterifying agent; On the other hand, the strong cavitation effect, mechanical effect, heat effect etc. that utilize ultrasonic wave to produce, promote esterifying agent to decompose and produce more-SO3H group, and then with-the OH on apple SDF, substitution reaction occurs, thus reach the object of esterification.3) to make esterification medium nonpoisonous and tasteless for water, and there is not organic reagent and remain, is a kind of safe, green esterification process.
Claims (8)
1., based on a preparation method for the sulphating apple water-soluble dietary fibre of water medium, it is characterized in that: comprise the following steps:
1) distilled water of apple dietary fiber and apple dietary fiber weight 20 ~ 40% is uniformly mixed leaves standstill 30 ~ 120min afterwards, after leaving standstill, carry out extrusion modification, then drying, apple dietary fiber extrusion modification thing of pulverizing and sieve to obtain successively;
2) add in apple dietary fiber extrusion modification thing after distilled water in 50 ~ 70 DEG C of lixiviate 1 ~ 2h, the mass ratio of apple dietary fiber extrusion modification thing and distilled water is 1:20 ~ 1:40, lixiviate terminates rear suction filtration and collects filtrate, ethanol is added and hold over night after filter vacuum is concentrated, then centrifugal and collecting precipitation, dries to obtain apple water-soluble dietary fibre by precipitation;
3) apple water-soluble dietary fibre is dissolved in distilled water and obtains mixture A, in mixture A, add thionamic acid under agitation and drip the vitriol oil, then be 20 ~ 40kHz in ultrasonic frequency, temperature is react 1 ~ 4h under the condition of 60 ~ 90 DEG C, room temperature is cooled to after reaction terminates, then be neutral by NaOH adjust ph, then ethanol is added and hold over night, then centrifugal and collecting precipitation, precipitation is dissolved in distilled water and loads in dialysis tubing, then distill water dialysis is used 2 ~ 4 days, then in dialyzate, ethanol is added and hold over night, then centrifugal and collecting precipitation, precipitation is dried to obtain sulphating apple water-soluble dietary fibre.
2. the preparation method of a kind of sulphating apple water-soluble dietary fibre based on water medium according to claim 1, is characterized in that: described extrusion modification is extruding puffing under screw speed is 700 ~ 900r/min condition.
3. the preparation method of a kind of sulphating apple water-soluble dietary fibre based on water medium according to claim 1, it is characterized in that: the condition of described vacuum concentration is: temperature is 45 ~ 60 DEG C, vacuum tightness is 0.06 ~ 0.09MPa, is concentrated into 1/4 ~ 1/6 of filtrate volume.
4. the preparation method of a kind of sulphating apple water-soluble dietary fibre based on water medium according to claim 1, is characterized in that: in described mixture A, the mass ratio of apple water-soluble dietary fibre and distilled water is 1:50 ~ 1:80.
5. the preparation method of a kind of sulphating apple water-soluble dietary fibre based on water medium according to claim 1, it is characterized in that: the mass ratio of described apple water-soluble dietary fibre and complex ester agent is 1:1 ~ 1:7, and complex ester agent is described thionamic acid and the vitriol oil.
6. the preparation method of a kind of sulphating apple water-soluble dietary fibre based on water medium according to claim 1, is characterized in that: the mass ratio of described thionamic acid and the vitriol oil is 1:0.5 ~ 1:4.
7. the preparation method of a kind of sulphating apple water-soluble dietary fibre based on water medium according to claim 1, it is characterized in that: the preparation method of described apple dietary fiber comprises the following steps: apple residue and distilled water are uniformly mixed rear NaOH and regulate pH to be 10 ~ 14, the mass ratio of apple residue and distilled water is 1:6 ~ 1:10, then mixture B is obtained in 50 ~ 80 DEG C of insulation 10min, the hydrogen peroxide of distilled water volume 1 ~ 3% is added to mixture B, then in 50 ~ 80 DEG C of stirring reaction 1 ~ 3h, stirring reaction terminates rear water displacement wash to neutral, drying successively after washing, pulverize and sieve, obtain apple dietary fiber.
8. the preparation method of a kind of sulphating apple water-soluble dietary fibre based on water medium according to claim 7, is characterized in that: the temperature of described oven dry is 35 ~ 60 DEG C; Described sieving all adopts 20 ~ 80 mesh sieves.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111567746A (en) * | 2020-06-08 | 2020-08-25 | 新疆农垦科学院 | Processing method of tomato-rich dietary fiber air-dried mutton chops |
| CN112971155A (en) * | 2021-03-29 | 2021-06-18 | 陕西农产品加工技术研究院 | Modified medlar residue dietary fiber chewable tablet and preparation method thereof |
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| CN101724087A (en) * | 2009-11-05 | 2010-06-09 | 浙江工业大学 | Method for preparing lentinan ester |
| KR20120073730A (en) * | 2010-12-27 | 2012-07-05 | 한국화학연구원 | Extraction method of water soluble, low molecular weight dietary fiber from lignocellulosic biomass |
| CN103610081A (en) * | 2013-11-01 | 2014-03-05 | 陕西科技大学 | Modification method of apple meal fiber |
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| CN1651466A (en) * | 2004-12-31 | 2005-08-10 | 中国药科大学 | Sulphatization derivative of polysaccharide, its preparation method and use |
| CN1844160A (en) * | 2006-03-15 | 2006-10-11 | 南京中医药大学 | Sulfonated changium root polysaccharides and preparing method and use thereof |
| CN101724087A (en) * | 2009-11-05 | 2010-06-09 | 浙江工业大学 | Method for preparing lentinan ester |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111567746A (en) * | 2020-06-08 | 2020-08-25 | 新疆农垦科学院 | Processing method of tomato-rich dietary fiber air-dried mutton chops |
| CN112971155A (en) * | 2021-03-29 | 2021-06-18 | 陕西农产品加工技术研究院 | Modified medlar residue dietary fiber chewable tablet and preparation method thereof |
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