CN104387425A - Method for condensation and separation of catalpa bungei essence Qiulingsu in catalpa bungei endophytic fungus broth and use thereof - Google Patents
Method for condensation and separation of catalpa bungei essence Qiulingsu in catalpa bungei endophytic fungus broth and use thereof Download PDFInfo
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- CN104387425A CN104387425A CN201410532262.2A CN201410532262A CN104387425A CN 104387425 A CN104387425 A CN 104387425A CN 201410532262 A CN201410532262 A CN 201410532262A CN 104387425 A CN104387425 A CN 104387425A
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Abstract
The invention discloses a method for condensation and separation of catalpa bungei essence Qiulingsu in catalpa bungei endophytic fungus broth and a use thereof. The method comprises the following steps of 1, adjusting a pH value of a catalpa bungei endophytic fungus broth to 4-10, 2, pre-cooling the catalpa bungei endophytic fungus broth subjected to pH adjustment to a pre-cooling temperature of 0-4 DEG C, 3, slowly cooling the catalpa bungei endophytic fungus broth in the case of crystal nucleus existence until the catalpa bungei endophytic fungus broth freezes, and 4, carrying out slow cooling freezing until the rest of the liquid broth is concentrated to a preset volume, stopping cooling and collecting the unfrozen liquid which is catalpa bungei essence Qiulingsu concentrate. The catalpa bungei endophytic fungus broth is concentrated to form the broth with 1/10 of the original volume and a catalpa bungei essence Qiulingsu yield is more than 90%. Compared with the prior art, the method effectively guarantees stabilization of catalpa bungei essence Qiulingsu in the raw material, and a high condensation yield, is free of any organic solvent and guarantees concentrate quality.
Description
Technical field
The present invention relates to biochemical field, be specifically related to a kind of method and application thereof of concentrating and separating Chinese catalpa spirit element from catalpa bungei endophytic fungi fermented liquid.
Background technology
Endogenetic fungus refers to that those lives are that inside plant tissues can't make plant produce the fungi of obvious disease.Endogenetic fungus can produce the same or analogous biologically active substance with host plant, has huge potential value, can become the source of new type anticancer medicine.One strain has the endogenetic fungus (CCTCC M 208102) of very strong anticancer vigor from separation and Extraction juglans mandshurica in this seminar, this bacterium of preliminary evaluation is Deuteromycotina, hyphomycetes, Moniliales, trichoderma, its fermented extracted liquid has very strong restraining effect to liver cancer cell.This is the exclusive brand-new bacterial classification in this laboratory, and it has the fermented liquid preparation method patented protection (ZL200810041214.8) of anti-cancer ability.Because the concentration of bioactive ingredients Chinese catalpa spirit element is lower in fermented liquid, after needs are concentrated, just effective later stage fine separation can be carried out.But Chinese catalpa spirit element is to temperature sensitive, the operation such as rotary evaporation causes a large amount of degraded of Chinese catalpa spirit element, and reverse phase absorption is again because Chinese catalpa spirit element can not effective wash-out and causing under concentration yield.
For above-mentioned technical problem, this invention exploits a kind of efficient concentration method to catalpa bungei endophytic fungi fermented liquid, ensureing concentrated more than 10 times of the situation decline fermented liquid of the plain yield of Chinese catalpa spirit, significantly can improve the follow-up plain fine separation process of Chinese catalpa spirit, be basis and the gordian technique of high efficiency separation purifying Chinese catalpa spirit element.
Summary of the invention
For defect of the prior art, the object of this invention is to provide a kind of method and application thereof of concentrating and separating Chinese catalpa spirit element from catalpa bungei endophytic fungi fermented liquid, it is a kind of method utilizing the clever element of concentrating and separating Chinese catalpa from catalpa bungei endophytic fungi fermented liquid that freezes, the method not only can realize the concentrating and separating of Chinese catalpa spirit element, also can be used for the concentrating and separating of other substance solutions.
The present invention is achieved by the following technical solutions:
The present invention relates to a kind of method of concentrating and separating Chinese catalpa spirit element from catalpa bungei endophytic fungi fermented liquid, said method comprising the steps of:
Step one, adjustment catalpa bungei endophytic fungi fermented liquid pH are 4 ~ 10;
Catalpa bungei endophytic fungi fermented liquid to precooling temperature after step 2, precooling pH adjustment is 0 ~ 4 DEG C;
Step 3, maintenance nucleus deposit in case to catalpa bungei endophytic fungi fermented liquid described in step 2 carry out slow cooling to freeze;
Step 4, continuation slow cooling freeze, until icing fermentating liquid volume and fermented liquid cumulative volume are than being set(ting)value; Stop cooling, collect the fermented liquid do not frozen, obtain the plain concentrated solution of Chinese catalpa spirit.
Preferably, described catalpa bungei endophytic fungi fermented liquid is according to method preparation described in patent ZL200810041214.8.
Preferably, in step one, the pH of described catalpa bungei endophytic fungi fermented liquid is 8 ~ 10.
Preferably, in step 2, the precooling temperature of described catalpa bungei endophytic fungi fermented liquid is 1 DEG C.
Preferably, in step 3, the heat transfer temperature difference in described slow cooling is not more than 6 DEG C;
More preferably, the heat transfer temperature difference in described slow cooling is 2 ~ 6 DEG C.
Preferably, in step 4, the heat transfer temperature difference in described slow cooling is not more than 4 DEG C;
More preferably, the heat transfer temperature difference in described slow cooling is 1 ~ 4 DEG C.
Preferably, in step 3, four, the mode of described slow cooling comprises and continues to add nucleus.
More preferably, described nucleus comprises 0 DEG C of trash ice.
Preferably, in step 3, four, the operation of described slow cooling is specially: the trash ice at every turn adding 1 milliliter 0 DEG C, again adds the trash ice of 1 milliliter 0 DEG C after trash ice melts completely.
In step 4, described set(ting)value can be determined according to technician's actual demand; Be specially: technician can experimentally demand, control fermented liquid and to freeze amount, thus obtain the volume of the fermented liquid that do not freeze of certain volume.
Second aspect, the present invention relates to a kind of application of method of concentrating and separating Chinese catalpa spirit element from catalpa bungei endophytic fungi fermented liquid, described application is specially, for concentrated, the separation of solute in solution.
Preferably, the solvent of described solution comprises water.
Compared with prior art, the present invention has following beneficial effect:
(1) the method for the invention concentration ratio is comparatively large, can realize fermented liquid more than 10 times and concentrate, and required time is short, without the need to continuous heating, and safe operation process;
(2) all operations operates all at low temperatures, ensure that the stable of Chinese catalpa spirit element;
(3) the method for the invention yield is high, and the yield of activeconstituents Chinese catalpa spirit element can reach more than 90%;
(4) the present invention adopts physical method, is completed, do not use any organic solvent, effectively prevent organic solvent pollution in whole operating process by natural icing mechanism, retains the original composition of fermented liquid, ensures the plain purity of Chinese catalpa spirit, and to operator's toxicological harmless.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in detail.Following examples will contribute to those skilled in the art and understand the present invention further, but not limit the present invention in any form.It should be pointed out that to those skilled in the art, without departing from the inventive concept of the premise, some distortion and improvement can also be made.These all belong to protection scope of the present invention.
In following embodiment, the preparation of catalpa bungei endophytic fungi fermented liquid comprises the steps:
One, the separation of endogenetic fungus: divide endogenetic fungus and adopt hyphal tip cutting method to carry out purifying;
Two, inducing spore produces: be inoculated on PDA substratum by the mould CCTCC M 208102 of long shoot wood, have the PDA substratum of mycelia to cut one piece, be put in long on CMX substratum, cultivate under room temperature, have spore to grow after 1 to 3 days;
Three, liquid fermentation and culture: picking spore is transferred to and is equipped with in PDA liquid nutrient medium, 26 ~ 28 DEG C, the concussion of 180rpm shaking table is cultivated, and stops fermentation, to obtain final product after 1 ~ 8 day.
embodiment 1
The present embodiment relates to the method for a kind of catalpa bungei endophytic fungi fermented liquid concentrating and separating Chinese catalpa spirit element, comprises the steps:
(1) adjusting fermented liquid pH is 4;
(2) fermented liquid is pre-chilled to 1 DEG C;
(3) heat transfer temperature difference set between fermented liquid and cold-trap is 4 DEG C of frozen fermented liquid, adds the trash ice of 1 milliliter 0 DEG C at every turn, again adds the trash ice of 1 milliliter 0 DEG C after trash ice melts completely, until fermented liquid starts to freeze;
(4) heat transfer temperature difference set between fermented liquid and cold-trap is 2 DEG C and continues frozen fermented liquid, until liquid state fermentation liquid amasss be reduced into original 1/10th, realizes 10 times and concentrates, stop freezing.
Remove ice cube, collect fermented liquid, find fermented liquid color burn, adopt HPLC method to measure the plain concentration of Chinese catalpa spirit in fermented liquid, calculating Chinese catalpa spirit element is 90% containing yield;
The present embodiment method achieves catalpa bungei endophytic fungi fermented liquid 10 times and concentrates in 90% yield situation.
embodiment 2
The present embodiment relates to the method for a kind of catalpa bungei endophytic fungi fermented liquid concentrating and separating Chinese catalpa spirit element, comprises the steps:
(1) adjusting fermented liquid pH is 8;
(2) fermented liquid is pre-chilled to 4 DEG C;
(3) heat transfer temperature difference set between fermented liquid and cold-trap is 6 DEG C of frozen fermented liquid, adds the trash ice of 1 milliliter 0 DEG C at every turn, again adds the trash ice of 1 milliliter 0 DEG C after trash ice melts completely, until fermented liquid starts to freeze.
(4) heat transfer temperature difference set between fermented liquid and cold-trap is 4 DEG C and continues frozen fermented liquid, until liquid state fermentation liquid amasss be reduced into original 1/15th, realizes 15 times and concentrates, stop freezing.
Remove ice cube, collect fermented liquid, find fermented liquid color burn, adopt HPLC method to measure the plain concentration of Chinese catalpa spirit in fermented liquid, calculating Chinese catalpa spirit element is 93% containing yield;
The present embodiment method achieves catalpa bungei endophytic fungi fermented liquid 15 times and concentrates in 93% yield situation.
embodiment 3
The present embodiment relates to a kind of catalpa bungei endophytic fungi fermented liquid concentrating and separating method, comprises the steps:
(1) adjusting fermented liquid pH is 10;
(2) fermented liquid is pre-chilled to 1 DEG C;
(3) heat transfer temperature difference set between fermented liquid and cold-trap is 2 DEG C of frozen fermented liquid, adds the trash ice of 1 milliliter 0 DEG C at every turn, again adds the trash ice of 1 milliliter 0 DEG C after trash ice melts completely, until fermented liquid starts to freeze.
(4) heat transfer temperature difference set between fermented liquid and cold-trap is 1 DEG C and continues frozen fermented liquid, until liquid state fermentation liquid amasss be reduced into original 1/15th, realizes 15 times and concentrates, stop freezing.
Remove ice cube, collect fermented liquid, find fermented liquid color burn, adopt HPLC method to measure the plain concentration of Chinese catalpa spirit in fermented liquid, calculating Chinese catalpa spirit element is 96% containing yield;
The present embodiment method achieves catalpa bungei endophytic fungi fermented liquid 15 times and concentrates in 96% yield situation.
embodiment 4
The present embodiment relates to a kind of catalpa bungei endophytic fungi fermented liquid concentrating and separating method, comprises the steps:
(1) adjusting fermented liquid pH is 8;
(2) fermented liquid is pre-chilled to 1 DEG C;
(3) heat transfer temperature difference set between fermented liquid and cold-trap is 2 DEG C of frozen fermented liquid, adds the trash ice of 1 milliliter 0 DEG C at every turn, again adds the trash ice of 1 milliliter 0 DEG C after trash ice melts completely, until fermented liquid starts to freeze.
(4) heat transfer temperature difference set between fermented liquid and cold-trap is 1 DEG C and continues frozen fermented liquid, until liquid state fermentation liquid amasss be reduced into original 1/20th, realizes 20 times and concentrates, stop freezing.
Remove ice cube, collect fermented liquid, find fermented liquid color burn, adopt HPLC method to measure the plain concentration of Chinese catalpa spirit in fermented liquid, calculating Chinese catalpa spirit element is 96% containing yield;
The present embodiment method achieves catalpa bungei endophytic fungi fermented liquid 20 times and concentrates in 96% yield situation.
embodiment 5
The present embodiment relates to a kind of catalpa bungei endophytic fungi fermented liquid concentrating and separating method, and technical scheme is identical with embodiment 4, and difference is, in step (2), precooling temperature is 0 DEG C.Adopt HPLC method to measure the plain concentration of Chinese catalpa spirit in fermented liquid, calculating Chinese catalpa spirit element is 92% containing yield.
comparative example 1
This comparative example is the comparative example of embodiment 4, and technical scheme is identical with embodiment 4, and difference is optimum configurations, and namely in step (1), the pH of fermented liquid is 3; Adopt HPLC method to measure the plain concentration of Chinese catalpa spirit in fermented liquid, calculating Chinese catalpa spirit element is 62% containing yield.
comparative example 2
This comparative example is the comparative example of embodiment 4, and technical scheme is identical with embodiment 4, and difference is optimum configurations, and namely in step (1), the pH of fermented liquid is 11; Adopt HPLC method to measure the plain concentration of Chinese catalpa spirit in fermented liquid, calculating Chinese catalpa spirit element is 80% containing yield.
comparative example 3
This comparative example is the comparative example of embodiment 4, and technical scheme is identical with embodiment 4, and difference is optimum configurations, and namely in step (2), the precooling temperature of fermented liquid is-1 DEG C; Adopt HPLC method to measure the plain concentration of Chinese catalpa spirit in fermented liquid, calculating Chinese catalpa spirit element is 64% containing yield.
comparative example 4
Under 60 DEG C and 0.07 normal atmosphere vacuum condition, rotate the endogenetic fungus fermented liquid of evaporation concentration 1 liter to 100 milliliters, find fermented liquid color burn, adopt HPLC method to measure the plain concentration of Chinese catalpa spirit in fermented liquid, calculating Chinese catalpa spirit element is 36% containing yield; This comparative example achieves catalpa bungei endophytic fungi fermented liquid 10 times and concentrates in 36% yield situation.
In sum, the method by freezing freezing process concentrating and separating catalpa bungei endophytic fungi fermented liquid of the present invention can realize the concentration ratio of more than 10 times, ensures the activeconstituents Chinese catalpa spirit cellulose content of more than 90% simultaneously.That the rotary evaporation overcoming comparatively high temps occurs when concentrating endogenetic fungus fermented liquid or forming component Chinese catalpa spirit element degraded and yield decline problem, the efficient concentration of Chinese catalpa spirit element in catalpa bungei endophytic fungi fermented liquid can be realized, for follow-up fine separation lays the foundation, it is one of gordian technique of Chinese catalpa spirit element separation.
Above specific embodiments of the invention are described.It is to be appreciated that the present invention is not limited to above-mentioned particular implementation, those skilled in the art can make various distortion or amendment within the scope of the claims, and this does not affect flesh and blood of the present invention.
Claims (8)
1. the method that the spirit of concentrating and separating Chinese catalpa is plain from catalpa bungei endophytic fungi fermented liquid, is characterized in that, said method comprising the steps of:
Step one, adjustment catalpa bungei endophytic fungi fermented liquid pH are 4 ~ 10;
Catalpa bungei endophytic fungi fermented liquid after step 2, precooling pH adjustment is to precooling temperature 0 ~ 4 DEG C;
Step 3, maintenance nucleus deposit in case to step 2 gained catalpa bungei endophytic fungi fermented liquid carry out slow cooling to freeze;
Step 4, continuation slow cooling freeze, until icing fermentating liquid volume and fermented liquid cumulative volume are than being set(ting)value; Stop cooling, collect the fermented liquid do not frozen, obtain the plain concentrated solution of Chinese catalpa spirit.
2. the method for concentrating and separating Chinese catalpa spirit element from catalpa bungei endophytic fungi fermented liquid according to claim 1, it is characterized in that, in step one, the pH of described catalpa bungei endophytic fungi fermented liquid is 8 ~ 10.
3. the method for concentrating and separating Chinese catalpa spirit element from catalpa bungei endophytic fungi fermented liquid according to claim 1, it is characterized in that, in step 2, the precooling temperature of described catalpa bungei endophytic fungi fermented liquid is 1 DEG C.
4. the method for concentrating and separating Chinese catalpa spirit element from catalpa bungei endophytic fungi fermented liquid according to claim 1, it is characterized in that, in step 3, the heat transfer temperature difference in described slow cooling is not more than 6 DEG C.
5. the method for concentrating and separating Chinese catalpa spirit element from catalpa bungei endophytic fungi fermented liquid according to claim 1, it is characterized in that, in step 4, the heat transfer temperature difference in described slow cooling is not more than 4 DEG C.
6. the method for concentrating and separating Chinese catalpa spirit element from catalpa bungei endophytic fungi fermented liquid according to claim 1, it is characterized in that, in step 3, four, the mode of described slow cooling comprises and continues to add nucleus.
7. the method for concentrating and separating Chinese catalpa spirit element from catalpa bungei endophytic fungi fermented liquid according to claim 6, it is characterized in that, described nucleus comprises 0 DEG C of trash ice.
8. the application of method of concentrating and separating Chinese catalpa spirit element from catalpa bungei endophytic fungi fermented liquid, described application is specially, for solute in solution concentrated, be separated.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113791651A (en) * | 2021-08-04 | 2021-12-14 | 南阳市京德啤酒技术开发有限公司 | Temperature control system for preventing fermentation tank from cooling and freezing |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008073914A2 (en) * | 2006-12-10 | 2008-06-19 | Dyadic International Inc. | Expression and high-throughput screening of complex expressed dna libraries in filamentous fungi |
| CN101487022B (en) * | 2008-07-31 | 2010-09-29 | 上海交通大学 | Preparation of fermentation liquor for inhibiting liver cancer cell growth |
| CN103756917A (en) * | 2014-01-14 | 2014-04-30 | 上海交通大学 | Preparation method of protoplasts of catalpa bungei endophytic fungi |
-
2014
- 2014-10-11 CN CN201410532262.2A patent/CN104387425B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008073914A2 (en) * | 2006-12-10 | 2008-06-19 | Dyadic International Inc. | Expression and high-throughput screening of complex expressed dna libraries in filamentous fungi |
| CN101487022B (en) * | 2008-07-31 | 2010-09-29 | 上海交通大学 | Preparation of fermentation liquor for inhibiting liver cancer cell growth |
| CN103756917A (en) * | 2014-01-14 | 2014-04-30 | 上海交通大学 | Preparation method of protoplasts of catalpa bungei endophytic fungi |
Non-Patent Citations (3)
| Title |
|---|
| 于湘莉 等: "楸树内生真菌抗癌性能研究", 《上海医药》 * |
| 吴运帷 等: "一株楸树内生真菌的鉴定及其发酵产物抗肿瘤特性研究", 《东北农业大学学报》 * |
| 李美芽 等: "一株具有抗HepG2活性的胡桃楸内生真菌的分离及研究", 《中国中药杂志》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113791651A (en) * | 2021-08-04 | 2021-12-14 | 南阳市京德啤酒技术开发有限公司 | Temperature control system for preventing fermentation tank from cooling and freezing |
| CN113791651B (en) * | 2021-08-04 | 2022-05-27 | 南阳市京德啤酒技术开发有限公司 | Temperature control system for preventing fermentation tank from cooling and freezing |
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