CN104383019B - The concocting method of anaesthetic Clematis aethusifolia - Google Patents
The concocting method of anaesthetic Clematis aethusifolia Download PDFInfo
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- CN104383019B CN104383019B CN201410586989.9A CN201410586989A CN104383019B CN 104383019 B CN104383019 B CN 104383019B CN 201410586989 A CN201410586989 A CN 201410586989A CN 104383019 B CN104383019 B CN 104383019B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
- A61K36/716—Clematis (leather flower)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/13—Preparation or pretreatment of starting material involving cleaning, e.g. washing or peeling
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/17—Preparation or pretreatment of starting material involving drying, e.g. sun-drying or wilting
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Abstract
The invention discloses the concocting method of anaesthetic Clematis aethusifolia, Clematis aethusifolia branches and leaves 500g is taken, remove impurity ash bits, be dried to obtain the net material of Clematis aethusifolia；180 ~ 220 DEG C of stir-frys are forged 5 ~ 28 minutes during the net material of Clematis aethusifolia is transferred into chamber type electric resistance furnace, are in coke black to surface, and Temperature fall weeds out ash and considers to obtain Clematis aethusifolia processed product to be worth doing.The present invention is easier to control temperature and time when processing medicinal material：Traditional concocting method forges method with vexed, and shortcoming is that time, temperature are difficult to control, and causes to process result badly, and the method utilizes chamber type electric resistance furnace, and heat time and temperature are easily controlled, thermal-stable, efficiency high；Toxicity reduction, increased its drug safety；" stir-baked the crude drugs into black on outside and brown in inside " aspect is more excellent, and this New function that stops blooding is increased on the basis of the drug effect of medicinal material script.
Description
Technical field
The present invention relates to middle anaesthetic preparation field, and in particular to the concocting method of anaesthetic Clematis aethusifolia.
Background technology
Clematis aethusifolia also known as Clematis aethusifolia Turcz, are Clematis, cohosh.It is distributed mainly on China Ling Dong, emerging
Annan portion, the Yanshan Mountain is northern, exhale a tin plateau, Wulanchabu League, the Yinshan Mountains, cloudy south hills, Erdos, Helan Mountain, Dragon Head Mountain, China
North, northwest, record in《Inner Mongol anaesthetic material standard》, its anaesthetic taste is pungent, micro-sweet, property is hot, light, sharp, dry, rough, poisonous, breaks ruffian,
Help temperature, dry " Xi Wu elements ", detumescence, corruption of dispelling, antidiarrheal, apocenosis.Chemical composition in Clematis aethusifolia includes caffeic acid, asafoetide
Acid, Quercetin etc., modern study show that Clematis aethusifolia has the effect such as analgesia, anti-inflammatory, antibacterial, antitumor.
Traditional concocting method of Clematis aethusifolia forges carbon for the vexed method of forging, and vexed method i.e. method of calcining with iron pot, the i.e. medicine forged lacks in high temperature
The method of charcoal is sintered under the conditions of oxygen.Clematis aethusifolia processed product that the method is obtained tests prove that it mainly contains Quercetin,
But content is very low（About 0.21mg/g）, other flavones ingredients are destroyed, and almost without content, cause its clinical application range
Narrow.
The content of the invention
It is an object of the invention to overcome the deficiencies in the prior art, and provide a kind of different from the more steady of traditional concocting method
The concocting method and processed product of fixed controllable anaesthetic Clematis aethusifolia, it remains the active ingredient of Clematis aethusifolia well,
Processed product toxicity reduction, quercetin content is significantly improved, and is provided with very strong anastalsis.
Technical scheme is as follows：
The concocting method of anaesthetic Clematis aethusifolia, takes Clematis aethusifolia branches and leaves 500g, removes impurity ash bits, is dried to obtain celery
The net material of leaf clematis；180 ~ 220 DEG C of stir-frys are forged 5 ~ 28 minutes during the net material of Clematis aethusifolia is transferred into chamber type electric resistance furnace, to table
Face is in coke black, and Temperature fall weeds out ash and considers to obtain Clematis aethusifolia processed product to be worth doing.
It is preferred that 18 ~ 200 DEG C of stir-frys are forged 5 ~ 18 minutes during the net material of Clematis aethusifolia is transferred into chamber type electric resistance furnace.
200 DEG C of stir-frys are forged 18 minutes during the net material of Clematis aethusifolia most preferably is transferred into chamber type electric resistance furnace.
The advantage of the invention is that：
It is easier to control temperature and time when the 1, processing medicinal material：Traditional concocting method forges method with vexed, shortcoming be the time,
Temperature is difficult to control, and causes to process result badly, and the method utilizes chamber type electric resistance furnace, and heat time and temperature are easily controlled,
Thermal-stable, efficiency high；2nd, toxicity reduction, increased its drug safety；3rd, " stir-baked the crude drugs into black on outside and brown in inside " aspect is more excellent, in medicinal material originally
Drug effect on the basis of increased hemostasis this New function.
The invention will be further described below specific embodiment.
The concocting method of anaesthetic Clematis aethusifolia, takes Clematis aethusifolia branches and leaves 500g, removes impurity ash bits, is dried to obtain celery
The net material of leaf clematis；180 ~ 220 DEG C of stir-frys are forged 5 ~ 28 minutes during the net material of Clematis aethusifolia is transferred into chamber type electric resistance furnace, to table
Face is in coke black, and Temperature fall weeds out ash and considers to obtain Clematis aethusifolia processed product to be worth doing.
Test example
Reagent and instrument：
Instrument：SX2-8-13 types chamber type electric resistance furnace, SPD-20A types high performance liquid chromatograph, DZTW type regulating temperature electrothermal covers
Reagent：Methyl alcohol is chromatographically pure, and it is pure that hydrochloric acid, absolute ethyl alcohol, phosphoric acid are analysis
Standard items：Quercetin standard items are purchased from National Institute for Food and Drugs Control
Clematis aethusifolia branches and leaves 500g is taken, impurity ash bits are removed, the net material of Clematis aethusifolia is dried to obtain；By parsley iron wire
The net material of lotus is forged 5,10,18,28 minutes in being transferred to chamber type electric resistance furnace in respectively at 180 DEG C, 200 DEG C, 220 DEG C of stir-frys, to surface
In coke black, Temperature fall weeds out ash and considers to obtain Clematis aethusifolia processed product to be worth doing.
Evaluation index：Overall merit is made with yield and content.
Comprehensive grading：
Comprehensive grading=yield/highest yield × 100 × 40%+ contents/highest content × 100 × 60%
1. assay
Sample treatment：Clematis aethusifolia processed product is taken in conical flask with cover, the absolute ethyl alcohol of 10% watery hydrochloric acid is added
50 times of amounts of solution, weighed weight is heated to reflux 30 minutes, lets cool, then weighed weight, with the ethanol solution of 10% watery hydrochloric acid
The weight of less loss is supplied, is shaken up, cross miillpore filter, HPLC analyses；
Chromatographic condition：C18 chromatographic columns, with volume ratio 52：48 phosphate aqueous solution of methyl alcohol -2% be mobile phase, 25 DEG C of column temperature,
Flow velocity 1.0ml/min, sampling volume 10ul, Detection wavelength is 360nm；
2. yield：
Yield（%）=（Weight before weight/processing after processing）×100%
3. Experiment Data Records are as follows：
| Temperature | The time is forged in stir-fry | Yield % | Quercetin content（mg/g) | Comprehensive grading |
| 180℃ | 5 minutes | 96.2 | 0.4543 | 47.47 |
| 180℃ | 10 minutes | 94.0 | 1.1412 | 57.84 |
| 180℃ | 18 minutes | 93.4 | 1.6285 | 65.60 |
| 180℃ | 28 minutes | 88.6 | 3.0228 | 86.53 |
| 200℃ | 5 minutes | 91.6 | 1.1443 | 56.90 |
| 200℃ | 10 minutes | 89.0 | 2.4109 | 76.64 |
| 200℃ | 18 minutes | 86.5 | 3.6502 | 95.97 |
| 200℃ | 28 minutes | 61.1 | 2.2572 | 67.68 |
| 220℃ | 5 minutes | 60.3 | 1.2013 | 44.82 |
| 220℃ | 10 minutes | 39.2 | 0.2376 | 20.21 |
| 220℃ | 18 minutes | 6.1 | --- | 2.54 |
| 220℃ | 28 minutes | 5.2 | --- | 2.16 |
When processing 18 minutes for 200 DEG C, comprehensive grading highest is 95.97 to Clematis aethusifolia, is both protected as can be seen from the above table
Yield has been demonstrate,proved again so that the content of Quercetin reaches highest.
Amplify production
100 kilograms of Clematis aethusifolia is taken, is processed as stated above, obtain 88.6 kilograms of Clematis aethusifolia processed products, yield
It is 88.6%, this processed product content is measured for 3.6489mg/g through experiment.
Toxicological test
Mouse 30 is taken, body weight 20g ± 1g, male and female half and half, adaptability is fed and observes 5 days to confirm health, random point
It is 3 groups, I group of experiment, experiment II group and blank control group, every group 10, fasting 12 hours, can't help water before experiment.Test I group with
Containing primary dose（Clematis aethusifolia health product）0.3g/ml gastric infusion 1ml, II group of experiment is with containing primary dose (Clematis aethusifolia
Processed product) 0.3g/ml gastric infusion 1ml, the 0.5%CMC-Na solution of blank control group gavage equivalent, period observes and records dynamic
Beyond the region of objective existence sight, appetite, behavioral activity, death condition.
Test I group of some animals and show excited uneasiness, spasm is cuddled up in a heap, and is had difficulty in breathing, then dead, and remaining
Mouse is rolled up in corner, and activity is reduced, but second day observes, and the activity of these mouse, drinking-water and feeding recover normal.And test
The aspects such as II group of spirit of mouse, appetite, fur, secretion, breathing, motion, do not observe ANOMALOUS VARIATIONS, with blank
Group nothing substantially observes difference.Continue to observe in 2 weeks, 2 weeks without dead animal, cervical vertebra execution mouse taken off at the 14th day and is tested,
Visually observe and do not find that the organs such as kidney, spleen, liver, lungs have swelling, bleeding or color exception etc. to change, according to toxicology
Standard, median lethal dose（LD50）＞ 10g/kg, belong to innocuous substance, and this experiment oral dose increases 15g/kg, experiment II
Group mouse is still good for and lives, it can be seen that, Clematis aethusifolia processed product substantially diminishes compared with health product toxicity, to the vital sign of mouse almost
Without influence.
Clematis aethusifolia processed product hemostatic function is tested
Mouse 20, male and female half and half are taken, adaptability is fed and observes 5 days to confirm health, is randomly divided into 2 groups, experimental group
With blank control group, every group 10, experimental group is with the processed product of processing 18min at 200 DEG C with containing primary dose 50mg/ml gavages
Administration 0.8ml, 2 times a day；The 0.5%CMC-Na solution of blank control group gavage equivalent, continuous 5 days, the 6th day morning dose one
It is secondary, the measure in bleeding time and clotting time is proceeded by after 40 minutes.
1. cut at Mouse Tail-tip 3mm with dissecting scissors, timing is started when blood voluntarily overflows, inhaled with filter paper within every 30 seconds
Blood once, until blood stop naturally as the mouse bleeding time.
2. use the capillary glass tube of internal diameter 1mm, 15cm long to insert ball rear vein beard in mouse left eye and take blood, after blood is filled
Take out, lie against desktop, fractureed capillary two ends about 0.5cm every 30 seconds, and slow left and right is pulled open, whether the observation place of fractureing has
Clotting strands, autoblood is flowed into and starts timing in capillary, seemingly occurring to blood clotting, when being the capillary end blood coagulation between lasting
Between, the average value for taking capillary two ends is the clotting time of the mouse.
Experiment Data Records are as follows：
| Type | Bleeding time（s） | Clotting time（s） |
| Blank control group | 72.31±9.27 | 82.16±16.39 |
| Experimental group | 53.87±8.56 | 60.11±13.62 |
As can be seen from the above table, the bleeding time and clotting time of experimental group are shorter than blank control group, illustrate parsley iron
Line lotus processed product has an impact to endogenous mouse blood coagulation system, it can be seen that, Clematis aethusifolia processed product has anastalsis.
Claims (2)
1. the concocting method of anaesthetic Clematis aethusifolia, it is characterised in that：Clematis aethusifolia branches and leaves 500g is taken, impurity ash bits are removed,
It is dried to obtain the net material of Clematis aethusifolia；By the net material of Clematis aethusifolia be transferred in chamber type electric resistance furnace 180 ~ 200 DEG C fry forge 5 ~
18 minutes, be in coke black to surface, and Temperature fall weeds out ash and considers to obtain Clematis aethusifolia processed product to be worth doing.
2. the concocting method of anaesthetic Clematis aethusifolia as claimed in claim 1, it is characterised in that：By the net material of Clematis aethusifolia
200 DEG C of stir-frys are forged 18 minutes in being transferred to chamber type electric resistance furnace.
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Address after: The town of Hongmiao Hongshan District, West Water Village 024000 the Inner Mongolia Autonomous Region Chifeng city (the Hongshan high tech Industrial Development Zone) Co-patentee after: Inner Mongolia Meng Tong Tang Pharmaceutical Technology Co., Ltd. Patentee after: INNER MONGOLIA TIANQI ZHONGMENG PHARMACEUTICAL CO., LTD. Co-patentee after: Inner Mongolia Lucao Food Technology Co., Ltd. Address before: The town of Hongmiao Hongshan District, West Water Village 024000 the Inner Mongolia Autonomous Region Chifeng city (the Hongshan high tech Industrial Development Zone) Co-patentee before: INNER MONGOLIA MENGJI PHARMACEUTICAL TECHNOLOGY CO., LTD. Patentee before: INNER MONGOLIA TIANQI ZHONGMENG PHARMACEUTICAL CO., LTD. Co-patentee before: CHIFENG LUER CAOZHONG MONGOLIAN MEDICINE TECHNOLOGY CO., LTD. |
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Effective date of registration: 20210301 Address after: No.75, niujiayingzi Road, niujiayingzi village, niujiayingzi Town, Harqin Banner, Chifeng City, Inner Mongolia Autonomous Region 024411 Patentee after: Inner Mongolia Meng Tong Tang Pharmaceutical Technology Co.,Ltd. Address before: 024000 xishuidi village, Hongmiaozi Town, Hongshan District, Chifeng City, Inner Mongolia Autonomous Region (Hongshan high tech Industrial Development Zone) Patentee before: INNER MONGOLIA TIANQI ZHONGMENG PHARMACEUTICAL Co.,Ltd. Patentee before: Inner Mongolia Meng Tong Tang Pharmaceutical Technology Co.,Ltd. Patentee before: Inner Mongolia Lucao Food Technology Co.,Ltd. |
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Denomination of invention: Processing method of Clematis apiifolia Effective date of registration: 20210624 Granted publication date: 20170606 Pledgee: Agricultural Bank of China Limited karaqin banner sub branch Pledgor: Inner Mongolia Meng Tong Tang Pharmaceutical Technology Co.,Ltd. Registration number: Y2021150000052 |