CN104382685B - A kind of preparation method of human vagina's probiotics-probio sheath - Google Patents
A kind of preparation method of human vagina's probiotics-probio sheath Download PDFInfo
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- CN104382685B CN104382685B CN201410621898.4A CN201410621898A CN104382685B CN 104382685 B CN104382685 B CN 104382685B CN 201410621898 A CN201410621898 A CN 201410621898A CN 104382685 B CN104382685 B CN 104382685B
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Abstract
A kind of preparation method of human vagina's probiotics probio sheath, it is characterized in that using the ecological theory of " with bacterium bacterium ", by adding probio in sheath front end or storage liquid, probio is made to be distributed in vagina during men and women makes love sufficiently uniformly, thus play recovery vaginal flora balance and self-cleaning function, effectively treat and prevent the gynecological diseases such as bacterial vaginitis (BV), colpomycosis, trichomonas vaginitis.The invention have the advantage that probio entrance vagina regulates the method for colony balance simply, conveniently, have treatment and safeguard function concurrently, strengthen the body resistance to consolidate the constitution, result for the treatment of is good, and after healing, nothing is repeatedly, and efficient, economy, safety are applied widely.
Description
Technical field
The present invention relates to the preparation method of a kind of human vagina probiotics-probio sheath.
Background technology
Lower Female Genital Tract is the Tiny ecosystem district that body weight for humans is wanted.In this Tiny ecosystem district, the vagina of normal women of child-bearing age is parasitic
200 multiple-microorganisms, they are grown on normal human vaginal's mucomembranous surface, composition microecology in vaginas system and dimension in a special way
Hold microecology in vaginas balance.When this microecology in vaginas system balancing is destroyed, there will be per vaginam flora imbalance and cause many
Plant disease, such as bacterial vaginitis (BV), colpomycosis, trichomonas vaginitis etc..Treat the measure master of vagina infection at present
If intravaginal pathogenic microorganism killed by the preparation etc. using antibiotic, metronidazole, estrogen and acidifying vagina, it is worth
It is noted that above-mentioned therapy can suppress normal flora to a certain extent, cause the excessive multiplication of pathogenic bacteria or conditioned pathogen,
Thus cause repeated infection or multiple infection, increase treatment difficulty and cost.Many studies have shown that, utilize the normal micro-life of vagina
The preparation that probio-Bacillus acidi lactici in state is made all has good curative effect to prevention and the treatment of vagina infection ", therefore, woman
Cause due to vaginal dysbacteriosis from the point of view of in section's colpitis theory, therefore should strengthen the body resistance to consolidate the constitution in treatment, at protection vagina
On the premise of the Tiny ecosystem ecological balance, treat and prevent so that it is recover the normal status in biocoene and effect.
At present, viable bacteria technology is widely used in the field such as food, medical science as a kind of common technology, but this technical point resolution is low,
Poor repeatability, depend on cultivation, therefore some growths are required that harsher microorganisms are difficult to monitoring.Therefore, originally grind
Study carefully and plan viable count method and high throughput sequencing technologies combines, cloudy to normal human vaginal and patient from Microflora and kind
Microorganism in road is furtherd investigate, and the research and development for prevention, treatment and the colpitis medicine of later stage colpitis class disease carry
For theoretical foundation.
Summary of the invention
Present invention aims to the deficiency of existing methods for the treatment of, it is provided that a kind of human vagina's probiotics-probio contraception
The preparation method of set.
The present invention is to utilize the dominant bacteria of normal microflora in woman vagina, cultivate, lyophilized after be fixed on the surface of sheath, in property
In life process, probio is applied in vagina perisporium the most fully, thus regulates microecology in vaginas balance.
Preparation method of the present invention comprises the following steps:
A, the cultivation of probio: select the probio after re-activation to be inoculated in culture medium and carry out cultivating 24-48 hour,
Described probio is: Lactobacillus crispatus, Lactobacillus Jensenii, lactobacillus gasseri, Lactobacillus vaginalis, Yue Shi lactobacillus, addicted to acid
One or more in lactobacillus or lactobacillus fermenti are combined；
Described probio inoculum concentration is probio and the 5% of culture medium cumulative volume；
B, probio lyophilized: the probio after cultivating uses centrifugal method to collect thalline, is placed in freeze dryer and is overnight lyophilized；
It is standby that collection freeze-dried powder is placed in-70 degree refrigerators；
C, the preparation of microcapsules: selection sodium alginate is capsule material, be auxiliary material with pectin, and liquid pectin concentration is 2%, liquid pectin with
Probio liquid volume ratio is 1:1, and sodium alginate and pectin mass ratio are 2:1, CaCL2Concentration is 0.3moL/L；Bifidobacterium
Microcapsule diameter is 1～2mm；
D, the preparation of probio sheath: prepare sheath by condom technology of preparing, can be by Freeze-dry Powder of Probioctics or micro-glue
The probio of encapsulated be fixed on sheath front end isolation cell in, this cell is placed in sheath top, by can completely cut off air and
Prepared by the special material of moisture, in order to extend probio holding time and survival rate in cell；During use, little locular wall can be by
Acidic materials and the degraded of other metabolites in vagina are broken, thus discharge probio, or by Freeze-dry Powder of Probioctics or micro-
Encapsulated probio is distributed in sheath and stores in liquid, thus in use it can be spread evenly across condom surface, finally
Can be distributed in and vagina reaches probiotic effects.
Beneficial effects of the present invention: by adding probio in sheath front end or storage liquid, make probio make love men and women
During be distributed in vagina sufficiently uniformly, thus play recovery vaginal flora balance and self-cleaning function, effectively treat and
Prevent the gynecological diseases such as bacterial vaginitis (BV), colpomycosis, trichomonas vaginitis.The invention have the advantage that prebiotic
The method that bacterium enters vagina regulation colony balance is simple, convenient, has treatment and safeguard function concurrently, strengthens the body resistance to consolidate the constitution, and result for the treatment of is good,
After healing, nothing is repeatedly, and efficient, economy, safety are applied widely.
Detailed description of the invention
Preparation method of the present invention comprises the following steps:
A, the cultivation of probio: select the probio after re-activation to be inoculated in culture medium and carry out cultivating 24-48 hour,
Described probio is: Lactobacillus crispatus, Lactobacillus Jensenii, lactobacillus gasseri, Lactobacillus vaginalis, Yue Shi lactobacillus, addicted to acid
One or more in lactobacillus or lactobacillus fermenti are combined；
Described probio inoculum concentration is probio and the 5% of culture medium cumulative volume；
B, probio lyophilized: the probio after cultivating uses centrifugal method to collect thalline, is placed in freeze dryer and is overnight lyophilized；
It is standby that collection freeze-dried powder is placed in-70 degree refrigerators；
C, the preparation of microcapsules: selection sodium alginate is capsule material, be auxiliary material with pectin, and liquid pectin concentration is 2%, liquid pectin with
Probio liquid volume ratio is 1:1, and sodium alginate and pectin mass ratio are 2:1, CaCL2Concentration is 0.3moL/L；Bifidobacterium
Microcapsule diameter is 1～2mm；
D, the preparation of probio sheath: prepare sheath by condom technology of preparing, can be by Freeze-dry Powder of Probioctics or micro-glue
The probio of encapsulated be fixed on sheath front end isolation cell in, this cell is placed in sheath top, by can completely cut off air and
Prepared by the special material of moisture, in order to extend probio holding time and survival rate in cell；During use, little locular wall can be by
Acidic materials and the degraded of other metabolites in vagina are broken, thus discharge probio, or by Freeze-dry Powder of Probioctics or micro-
Encapsulated probio is distributed in sheath and stores in liquid, thus in use it can be spread evenly across condom surface, finally
Can be distributed in and vagina reaches probiotic effects.
Claims (3)
1. a preparation method for human vagina's probiotics-probio sheath, is characterized in that:
A, the cultivation of probio: select the probio after re-activation to be inoculated in culture medium and carry out cultivating 24-48 hour,
B, probio lyophilized: the probio after cultivating uses centrifugal method to collect thalline, is placed in freeze dryer and is overnight lyophilized；
It is standby that collection freeze-dried powder is placed in-70 degree refrigerators；
C, the preparation of microcapsules: selection sodium alginate is capsule material, be auxiliary material with pectin, and liquid pectin concentration is 2%, liquid pectin with
Probio liquid volume ratio is 1:1, and sodium alginate and pectin mass ratio are 2:1, CaCL2Concentration is 0.3moL/L；Bifidobacterium
Microcapsule diameter is 1～2mm；
D, the preparation of probio sheath: prepare sheath by condom technology of preparing, can be by Freeze-dry Powder of Probioctics or micro-glue
The probio of encapsulated be fixed on sheath front end isolation cell in, this cell is placed in sheath top, by can completely cut off air and
Prepared by the special material of moisture, in order to extend probio holding time and survival rate in cell；During use, little locular wall can be by
Acidic materials and the degraded of other metabolites in vagina are broken, thus discharge probio, or by Freeze-dry Powder of Probioctics or micro-
Encapsulated probio is distributed in sheath and stores in liquid, thus in use it can be spread evenly across condom surface, finally
Can be distributed in and vagina reaches probiotic effects.
The preparation method of a kind of human vagina's probiotics-probio sheath the most according to claim 1, is characterized in that: described probio
It it is the one in Lactobacillus crispatus, Lactobacillus Jensenii, lactobacillus gasseri, Lactobacillus vaginalis, Yue Shi lactobacillus, lactobacillus acidophilus or lactobacillus fermenti
Or it is several compound.
The preparation method of a kind of human vagina's probiotics-probio sheath the most according to claim 1, is characterized in that:
Described probio inoculum concentration is probio and the 5% of culture medium cumulative volume.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410621898.4A CN104382685B (en) | 2014-11-07 | 2014-11-07 | A kind of preparation method of human vagina's probiotics-probio sheath |
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| CN201410621898.4A CN104382685B (en) | 2014-11-07 | 2014-11-07 | A kind of preparation method of human vagina's probiotics-probio sheath |
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| CN104382685A CN104382685A (en) | 2015-03-04 |
| CN104382685B true CN104382685B (en) | 2016-08-24 |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105193855A (en) * | 2015-09-25 | 2015-12-30 | 南昌大学 | Method for application of natural vaginal flora transplantation in treating gynecological inflammation |
| CN105193860A (en) * | 2015-09-25 | 2015-12-30 | 南昌大学 | Method for application of artificially-cultured vaginal flora transplantation in treating gynecological inflammation |
| CN106562998A (en) * | 2015-10-12 | 2017-04-19 | 深圳华大基因研究院 | Applications of lactobacillus crispatus in treatment or prevention of uterus myoma related diseases |
| CN106823013A (en) * | 2016-12-31 | 2017-06-13 | 天津瑞特医疗保健用品开发有限公司 | Biological mycoderm and preparation method thereof, maintenance method and application |
| CN106943635B (en) * | 2017-03-09 | 2020-12-04 | 南昌大学 | Preparation method of human body lubricant based on probiotic culture |
| CN110777087B (en) * | 2019-08-09 | 2020-08-07 | 四川厌氧生物科技有限责任公司 | Lactobacillus johnsonii and application thereof |
| CN113292843A (en) * | 2021-06-07 | 2021-08-24 | 经鸿纬健康科技发展(上海)有限公司 | Lactobacillus-containing polyurethane condom capable of adjusting organism immunity function |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101028289A (en) * | 2006-03-01 | 2007-09-05 | 大连森佰澳科技有限公司 | Probiotics preparation for adjusting microbial pool, preventing or treating bacterial vaginosis |
| CN101048168A (en) * | 2004-10-05 | 2007-10-03 | 普罗比公司 | Probiotic lactobacillus strains for improved vaginal health |
| CN103110665A (en) * | 2013-01-24 | 2013-05-22 | 量子高科(中国)生物股份有限公司 | Composition for maintaining genital tract micro-ecological barrier and application thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014020613A1 (en) * | 2012-07-30 | 2014-02-06 | Plantus Health Supplements Private Limited | Probiotic layered condom |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101048168A (en) * | 2004-10-05 | 2007-10-03 | 普罗比公司 | Probiotic lactobacillus strains for improved vaginal health |
| CN101028289A (en) * | 2006-03-01 | 2007-09-05 | 大连森佰澳科技有限公司 | Probiotics preparation for adjusting microbial pool, preventing or treating bacterial vaginosis |
| CN103110665A (en) * | 2013-01-24 | 2013-05-22 | 量子高科(中国)生物股份有限公司 | Composition for maintaining genital tract micro-ecological barrier and application thereof |
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