CN104374769B - A kind of method of paper substrate colorimetric detection glucose - Google Patents
A kind of method of paper substrate colorimetric detection glucose Download PDFInfo
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- CN104374769B CN104374769B CN201410651780.6A CN201410651780A CN104374769B CN 104374769 B CN104374769 B CN 104374769B CN 201410651780 A CN201410651780 A CN 201410651780A CN 104374769 B CN104374769 B CN 104374769B
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Abstract
The invention discloses a kind of method of paper substrate colorimetric detection glucose, comprise the following steps：Self-control colorimeter；Design hydrophobic wax print pattern；Cut filter paper；Hydrophobic wax print pattern is printed on filter paper；Dewaxing is molded；Prepare ZnFe2O4；Prepare mixed solution；Mixed solution is added dropwise in detection cell；Detected solution is added dropwise；Folio；Detected using self-control colorimeter.The method of detection glucose of the invention is simple to operate, is operable without professional and technical personnel, and detection speed is fast, with low cost, and post processing is simple pollution-free.
Description
Technical field
The present invention relates to a kind of method of paper substrate colorimetric detection glucose, belong to the detection technique field of glucose.
Background technology
Used as the main component of blood in human body in sugar, the glucose energy source main for physical activity is provided, its level is high
The low health for directly affecting people.Wherein, hyperglycaemia is further serious to harm that health is produced, such as produces fat
The diseases such as disease, diabetes.At present, diabetes have turned into and have been only second to cancer and angiocardiopathy, threaten the third-largest of human health
Disease, showed according to related international organization in the investigation result of 2010, and the diabetic in the whole world has been up to 2.85 hundred million, every year
The number of diabetic is still constantly riseing.Meanwhile, diabetes can also produce multiple complications, such as cerebral infarction, cerebral hemorrhage, height
Blood pressure etc., indirectly or directly affects the life security of individuality, and carry out monitor in real time to the state of an illness of diabetic is with treatment
Ensure an effective way of diabetic's life security, and Diabetes Mellitus are carried out with Real Time Observation with detection most
Effective means is the concentration for determining diabetic's glucose in blood.Therefore, to the analysis of concentration of glucose with detection to people
The health of body and the diagnosis of disease, treatment and control important in inhibiting.
The method for determining glucose at present mainly has AAS, Optical Rotation, gas chromatography, high performance liquid chromatography
Method, electrochemica biological sensor method etc..Although these methods all have the advantages that different, but still deficiency is there is, for example, gone up
Stating method detection is carried out to concentration of glucose needs specific equipment, equipment in itself costly, without economy；Need simultaneously
Professional technician operating instrument is wanted, without due universality.In addition, the above method determine the cycle it is long, analysis process compared with
It is complexity, it is impossible to realize the real time measure of glucose.
The content of the invention
In view of the above-mentioned problems in the prior art, the technical problems to be solved by the invention there is provided a kind of operation
Simply, the method for fast, the with low cost paper substrate colorimetric detection glucose of detection speed.
In order to solve the above-mentioned technical problem, present invention employs following technical scheme：A kind of simple to operate, detection speed
Hurry up, the method for paper substrate colorimetric detection glucose with low cost, it is characterized in that comprising the following steps：
1. colorimeter is made by oneself, and schematic diagram is as shown in Figure 1.
2. Software for Design hydrophobic wax print pattern is used on computers, and pattern is as shown in Figure 2.
3. filter paper is cut into A4 sizes.
4. the A4 filter paper that will be cut out in step 3 is placed into wax spray printer, the hydrophobic wax printing that will be designed in step 2
Pattern is printed on the A4 filter paper in step 3.
5. the A4 filter paper with hydrophobic wax pattern that will be obtained in step 4 makes in 0.5-2 min are heated at 60-150 DEG C
Wax melts and is impregnated with the thickness of whole paper, forms hydrophobic wall.
6. the method reported using document prepares ZnFe2O4。
7. mixed solution is prepared.Mixed solution is by the U/mL glucose oxidases of 20 μ L 100, the mol/L of 20 μ L 0.2
Trehalose, the mg/mL ZnFe of 20 μ L 0.52O4, 20 μ L 20,3,3 ', 5,5 '-tetramethyl benzidines of mM and 60 μ L pH 4
Cushioning liquid constituted.
8. 10 μ L mixed solutions are taken in detection cell 1, and are dried naturally.
9. the solution to be measured that 20 μ L contain glucose is taken to be added dropwise in flow region top 2, after liquid flows completely through passage,
By paper part A along dotted line doubling in paper B tops.
10. detection zone is placed in the color acquisition window of self-control colorimeter, operation colorimeter is gathered and reads face after 10 seconds
Color shade value.
Present invention additionally comprises following steps：
Self-control colorimeter of the present invention is by color sensor, 32 arm processors, LED light-supplementing systems, liquid crystal display systems
System and the part of keyboard five are constituted.
Design layout software of the present invention is Adobe Illustrator CS4 or Adobe Photoshop CS6.
Filter paper of the present invention is conventional filter paper.
Mode of heating of the present invention is heating plate or baking oven.
The a diameter of 3-6 mm of detection cell of the present invention, the flow region top end diameter is 4-8 mm, and flow region leads to
Road width is 3-6 mm, and passage length is 20-40 mm.
Paper part A detection cell number of the present invention is 8-16.
The overall size of paper device of the present invention is 25 mm × 80 mm ~ 50 mm × 120 mm.
Beneficial effects of the present invention：
1. a kind of method of paper substrate colorimetric detection glucose is measured using self-control colorimeter, without expensive instrument
Device equipment, reduces experimental cost.
2. it is measured using colorimeter, it is simple to operate, it is operable without professional and technical personnel.
3. paper is typically white, and detection background is low, is conducive to carrying out colorimetric analysis.
4. post-process simply, it is pollution-free.Paper is flammable, after paper device has been used, can be by simple and safe combustion method
Processed, environment will not be polluted.
5. the sample of each concentration is determined 4 times and averaged simultaneously, reduces error.
Brief description of the drawings
Fig. 1 is self-control colorimeter schematic diagram.The operating procedure of this device is that abundant reacted paper device is placed in into camera bellows
In, the collection head of color acquisition window is regulated, close camera bellows；Turn on the power switch, display screen display system is initialized to
Work(, presses collection key, waits, and display screen Show Color is recognized successfully, and Show Color gray value.
Fig. 2 is hydrophobic wax print pattern.Wherein white portion is hydrophilic area, and black portions are wax print area.Part A is
Detection zone, part B is liquid flow region.
Specific embodiment
The present invention is described in further detail with specific embodiment below in conjunction with the accompanying drawings.
Embodiment 1 (glucose in water)
A kind of method of fast, the with low cost paper substrate colorimetric detection glucose of simple to operate, detection speed, including following step
Suddenly：
1. colorimeter is made by oneself, and schematic diagram is as shown in Figure 1.
2. Adobe Illustrator CS4 Software for Design hydrophobic wax print patterns are used on computers, and pattern is as schemed
Shown in 2.
3. filter paper is cut into A4 sizes.
4. the A4 filter paper that will be cut out in step 3 is placed into wax spray printer, the hydrophobic wax printing that will be designed in step 2
Pattern is printed on the A4 filter paper in step 3.
5. the A4 filter paper with hydrophobic wax pattern that will be obtained in step 4 is placed into heating plate in heating 1.5 at 90 DEG C
Min, melts wax and is impregnated with the thickness of whole paper, forms hydrophobic wall.
6. the method reported using document prepares ZnFe2O4。
7. mixed solution is prepared.Mixed solution is by the U/mL glucose oxidases of 20 mL 100, the mol/L of 20 mL 0.2
Trehalose, the mg/mL ZnFe of 20 mL 0.52O4, 20 mL 20,3,3 ', 5,5 '-tetramethyl benzidines of mM and 60 mL pH 4
Cushioning liquid constituted.
8. 10 mL mixed solutions are taken in a diameter of 3 mm detection cells 1, and are dried naturally.
9. take the aqueous solution to be measured that 20 mL contain glucose to be added dropwise in the flow region top 2 of a diameter of 5 mm, liquid
Body flows completely through width for 3 mm, and length is in paper B tops after 25 mm passages by paper part A along dotted line doubling.
10. detection zone is placed in the color acquisition window of self-control colorimeter, operation colorimeter is gathered and reads face after 10 seconds
Color shade value.
1 is the results are shown in Table using what the inventive method was measured.Use blood sugar analyzer simultaneously（SBA-40）To same to be measured
The aqueous solution is determined, and the results are shown in Table 1.
Glucose measurement (n=5) in the aqueous solution to be measured of table 1.
Be can be seen that from the result in table 1：The method and blood sugar analyzer of a kind of paper substrate colorimetric detection glucose of the present invention
Measurement result closely.
Embodiment 2（Glucose in serum）
A kind of method of fast, the with low cost paper substrate colorimetric detection glucose of simple to operate, detection speed, including following step
Suddenly：
1. colorimeter is made by oneself, and schematic diagram is as shown in Figure 1.
2. Adobe Photoshop CS6 Software for Design hydrophobic wax print patterns, pattern such as Fig. 2 are used on computers
It is shown.
3. filter paper is cut into A4 sizes.
4. the A4 filter paper that will be cut out in step 3 is placed into wax spray printer, the hydrophobic wax printing that will be designed in step 2
Pattern is printed on the A4 filter paper in step 3.
5. the A4 filter paper with hydrophobic wax pattern that will be obtained in step 4 is placed into baking oven in heating 0.5 at 150 DEG C
Min, melts wax and is impregnated with the thickness of whole paper, forms hydrophobic wall.
6. the method reported using document prepares ZnFe2O4。
7. mixed solution is prepared.Mixed solution is by the U/mL glucose oxidases of 20 mL 100, the mol/L of 20 mL 0.2
Trehalose, the mg/mL ZnFe of 20 mL 0.52O4, 20 mL 20,3,3 ', 5,5 '-tetramethyl benzidines of mM and 60 mL pH 4
Cushioning liquid constituted.
8. 10 mL mixed solutions are taken in a diameter of 4 mm detection cells 1, and are dried naturally.
9. the test serum solution that 20 mL contain glucose is taken to be added dropwise in the flow region top 2 of a diameter of 6 mm,
Liquid flows completely through width for 4 mm, and length is in paper B tops after 35 mm passages by paper part A along dotted line doubling.
10. detection zone is placed in the color acquisition window of self-control colorimeter, operation colorimeter is gathered and reads face after 10 seconds
Color shade value.
2 are the results are shown in Table using what the inventive method was measured.Use blood sugar analyzer simultaneously（SBA-40）To same to be measured
Serum solution is determined, and the results are shown in Table 2.
Test serum glucose in solutions measurement result (n=5) of table 2.
Be can be seen that from the result in table 2：The method and blood sugar analyzer of a kind of paper substrate colorimetric detection glucose of the present invention
Measurement result closely, can be applied to the measure of actual sample.
Claims (4)
1. a kind of method of paper substrate colorimetric detection glucose, it is characterized in that comprising the following steps：
1.1. colorimeter is made by oneself, the colorimeter is by color sensor, 32 arm processors, LED light-supplementing systems, liquid crystal display systems
System and the part of keyboard five are constituted；
1.2. it is hydrophobic using Adobe Illustrator CS4 or Adobe Photoshop CS6 Software for Design on computers
Wax print pattern;
1.3. filter paper is cut into A4 sizes;
1.4. the A4 filter paper that will be cut out in step 1.3 is placed into wax spray printer, the hydrophobic wax printing that will be designed in step 1.2
Pattern is printed on the A4 filter paper in step 1.3;
1.5. the A4 filter paper with hydrophobic wax pattern that will be obtained in step 1.4 makes wax in 0.5-2 min are heated at 60-150 DEG C
Melt and be impregnated with the thickness of whole paper, form hydrophobic wall, obtain paper device;
1.6. the method reported using document prepares ZnFe2O4;
1.7. mixed solution is prepared；Mixed solution is by the U/mL glucose oxidases of 20 μ L 100, the mol/L marine algas of 20 μ L 0.2
Sugar, the mg/mL ZnFe of 20 μ L 0.52O4, 20 μ L 20,3,3 ', 5,5 '-tetramethyl benzidines of mM and 60 μ L pH 4 it is slow
Solution is rushed to be constituted;
1.8. 10 μ L mixed solutions are taken in the detection cell of paper device（1）In, and dry naturally；The a diameter of 3-6 mm of detection cell；Paper
Device part A detection cell number is 8-16；
1.9. the solution to be measured that 20 μ L contain glucose is taken to be added dropwise in flow region top（2）, liquid flows completely through flow region
After passage by paper device part A along dotted line doubling in paper device part B top；Flow region top end diameter is 4-8 mm, flowing
Regional channel width is 3-6 mm, and flow region passage length is 20-40 mm；
1.10.10 abundant reacted paper device is placed in camera bellows after the second, regulates the collection head of color acquisition window, closed
Good camera bellows；Turn on the power switch, display screen display system is initialized to work(, presses collection key, waits, and display screen Show Color is known
Not Cheng Gong, and Show Color gray value, the sample of each concentration is determined 4 times simultaneously averages.
2. a kind of method of paper substrate colorimetric detection glucose according to claim 1, it is characterized in that：The filter paper is conventional filter
Paper.
3. a kind of method of paper substrate colorimetric detection glucose according to claim 1, it is characterized in that：The mode of heating is to add
Hot plate or baking oven.
4. a kind of method of paper substrate colorimetric detection glucose according to claim 1, it is characterized in that：Total chi of the paper device
Very little is 25 mm × 80 mm ~ 50 mm × 120 mm.
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| CN104374769B true CN104374769B (en) | 2017-06-23 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106092982B (en) * | 2016-06-02 | 2018-08-14 | 济南大学 | The preparation of cancer cell and cell surface polysaccharide detection fluorescence/colorimetric double mode paper chip |
| CN106526199B (en) * | 2016-11-02 | 2018-06-05 | 济南大学 | A kind of structure of the paper analysis device based on portable glucose meter detection fibrin ferment |
| CN108732167A (en) * | 2017-04-17 | 2018-11-02 | 天津大学 | The method that rapid field detection is carried out to Polychlorinated biphenyls total amount with papery detection device |
| CN107356588A (en) * | 2017-06-30 | 2017-11-17 | 济南大学 | The superoxide radical and hydrogen peroxide paper substrate detection method of a kind of simple and sensitive |
| CN107561070B (en) * | 2017-08-28 | 2018-11-13 | 菏泽学院 | A kind of glucose quick visualization detection method and its preparation and application |
| CN110591904A (en) * | 2019-09-25 | 2019-12-20 | 福州大学 | Device for detecting miRNA |
| CN111796016A (en) * | 2020-07-13 | 2020-10-20 | 济南大学 | Method for detecting glucose by electrochemical/colorimetric dual-mode sensor |
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| JP4846467B2 (en) * | 2006-06-30 | 2011-12-28 | 株式会社東芝 | Color reaction detection device and method for producing the same |
| CN101759146B (en) * | 2010-01-20 | 2013-04-17 | 浙江师范大学 | Method for preparing ZnO/ZnFe2O4 compound nano hollow sphere |
| CN103575728B (en) * | 2012-08-01 | 2016-12-21 | 中国科学院大连化学物理研究所 | Based on filter paper, colorimetric sensor array is used for trace heavy metal ion detection method |
| CN103018231A (en) * | 2012-12-27 | 2013-04-03 | 济南大学 | Preparation method and application of composite nano material paper chip electrochemical luminescence immunosensor |
| CN103041876B (en) * | 2012-12-27 | 2014-09-03 | 济南大学 | Preparation of electrochemical three-dimensional microfluidic paper chip and application of electrochemical three-dimensional microfluidic paper chip to field test |
| CN103103634B (en) * | 2013-01-05 | 2014-11-05 | 浙江大学 | ZnFe2O4 nano particle and ZnO nanofiber composite nanomaterial for glucose color index sensing and preparation method thereof |
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