CN104371033A - Method for extracting polysaccharides from Monascus purpureus fermentation waste water - Google Patents
Method for extracting polysaccharides from Monascus purpureus fermentation waste water Download PDFInfo
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- CN104371033A CN104371033A CN201410460410.4A CN201410460410A CN104371033A CN 104371033 A CN104371033 A CN 104371033A CN 201410460410 A CN201410460410 A CN 201410460410A CN 104371033 A CN104371033 A CN 104371033A
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Abstract
The present invention belongs to the technical field of Monascus purpureus waste water treatment, specifically to a method for extracting polysaccharides from Monascus purpureus fermentation waste water. According to the present invention, with the alcohol extraction method, the waste water generated by the Monascus purpureus fermentation is comprehensively utilized to extract the effective component polysaccharide, wherein the polysaccharide is used for preparation of drugs or health food for prevention of cardiovascular diseases and cancer diseases.
Description
Technical field
The invention belongs to the technical field of monascus ruber wastewater treatment, be specially the method extracting polysaccharide in Fermentation Condition of Monascus spp waste water.
Background technology
Monascus ruber has the ability producing exocellular polysaccharide, and this polysaccharide is the high molecular polymer be formed by connecting with glycosidic link by the monose of more than ten, there is obvious biologic activity, most important immunoregulatory activity, be widely used in clinical treatment and the field of medicaments of immune deficiency disorder and tumor disease, become the study hotspot of molecular biology, medicine and pharmacology.Containing a large amount of polysaccharide in the fermented liquid of monascus deep fermentation, the Crude polysaccharides obtained through centrifugal, alcohol precipitation and biomass have broad sense in the field of study, particularly in drugs against tumor, the antitumor activity of Monascus polysaccharides is mediated the impact of host body by Immunization Activities, and water-soluble polysaccharide plays prior effect, polysaccharide molecular weight is larger, antitumor activity is stronger, can also cardiac stimulant step-down, prevention and therapy atherosclerosis, its mechanism is the insulin level owing to improve blood plasma, accelerates glucose metabolism.Oral or abdominal injection Monascus polysaccharides, can also strengthen and be delayed over sensitization to proteantigen, promotes the synthesis of protein and nucleic acid.
At present, biological polyoses is mainly from the extract of mushroom, yeast, and current most of producer Fermentation Condition of Monascus spp waste water does not reclaim again, yet there are no and extracts the relevant report of polysaccharide.
Summary of the invention
The object of the present invention is to provide a kind of method extracting polysaccharide in Fermentation Condition of Monascus spp waste water, the waste water that Fermentation Condition of Monascus spp produces is fully utilized, extracting effective components polysaccharide, for the preparation of the medicine or the protective foods that prevent cardiovascular and cerebrovascular and tumor disease.
Technical scheme of the present invention is:
Extract the method for polysaccharide in Fermentation Condition of Monascus spp waste water, step is as follows:
By the product liquid that Fermentation Condition of Monascus spp produces, by squeezing machine, moisture is extruded, obtain Fermentation Condition of Monascus spp waste water, waste water and ethanol are mixed in proportion into the solution that mass concentration is 70%-80% alcohol, refrigerator 0-10 DEG C of precipitated crystal 16-32h, centrifugal, by supernatant liquor alcohol precipitation again, twice precipitation merges, dry concentrated, obtains Crude polysaccharides;
Described Crude polysaccharides is dissolved in the water by mass volume ratio 1:5, and molecular sieve adsorption is decoloured, centrifugal, gets supernatant liquor and is mixed in proportion into ethanol the solution alcohol precipitation that mass concentration is 70%-80% alcohol, is dried by pelleting centrifugation, obtains smart polysaccharide.
Described Tc is preferably 0-4 DEG C.
Described crystallization time is preferably 20-28h.
Described Fermentation Condition of Monascus spp waste water, get rice, peptone raw material monascus through liquid submerged fermentation after 6 to 7 days, the product liquid of the Fermentation Condition of Monascus spp produced, by squeezing machine, moisture is extruded, obtain Fermentation Condition of Monascus spp waste water, wherein monascus adopts the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, is numbered the red colouring agent for food, also used as a Chinese medicine bacterial classification of CGMCCNo.6806.
Wherein, be numbered the red colouring agent for food, also used as a Chinese medicine bacterial classification of CGMCCNo.6806, it is in the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, address: Datun Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica, postcode: 100101, the Latin title of bacterial classification is red colouring agent for food, also used as a Chinese medicine Monascus sp., the microorganism (strain) of ginseng certificate: ZG-m1, preservation date is on November 13rd, 2012.
The smart polysaccharide that the present invention obtains also can be called Monascus polysaccharides.
The smart polysaccharide that the present invention obtains, can be used as medicine or foodstuff additive.
The smart polysaccharide that the present invention obtains, for the preparation of the medicine or the protective foods that prevent cardiovascular and cerebrovascular and tumor disease.
The smart polysaccharide that the present invention obtains, by traditional Chinese medicine production technique, can make tablet, capsule, granule, and electuary etc. for the pharmaceutical dosage form of clinical application, can be applied for the patient clinical suffering from cardiovascular and cerebrovascular diseases.
Beneficial effect of the present invention is: the method extracting polysaccharide in Fermentation Condition of Monascus spp waste water of the present invention, and by arranging rational processing parameter, in 1L waste water, the content of Crude polysaccharides is about 12 g, often criticizes generation about 40 m
3waste water, 1m
3waste water can output 1Kg Monascus polysaccharides, 40 m
3waste water can output 40 Kg Monascus polysaccharides, monthly by 15 batches of calculating, can produce Monascus polysaccharides 600 Kg, and market value is pressed 300 yuan/Kg and calculated, and can produce 180,000 yuan of total values, profit margin is huge.
While extraction monascorubin, the exocellular polysaccharide in waste water can be extracted, not only there is environmental benefit, considerable economic benefit can also be brought.
Table 1
embodiment
Below by specific embodiment, technical scheme of the present invention is described in detail.
Described Fermentation Condition of Monascus spp waste water, get rice, peptone raw material monascus through liquid submerged fermentation after 6 to 7 days, the product liquid of the Fermentation Condition of Monascus spp produced, by squeezing machine, moisture is extruded, obtain Fermentation Condition of Monascus spp waste water, wherein monascus adopts the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, is numbered the red colouring agent for food, also used as a Chinese medicine bacterial classification of CGMCCNo.6806.
embodiment 1
Extract the method for polysaccharide in Fermentation Condition of Monascus spp waste water, step is as follows:
By the product liquid that Fermentation Condition of Monascus spp produces, by squeezing machine, moisture is extruded, obtain Fermentation Condition of Monascus spp waste water, waste water and ethanol are mixed in proportion into the solution that mass concentration is 70% alcohol, refrigerator 4 DEG C of precipitated crystal 24h, centrifugal, by supernatant liquor alcohol precipitation again, twice precipitation merges, dry concentrated, obtains Crude polysaccharides; Described Crude polysaccharides is dissolved in the water by mass volume ratio 1:5, and molecular sieve adsorption is decoloured, centrifugal, gets supernatant liquor and is mixed in proportion into ethanol the solution alcohol precipitation that mass concentration is 70% alcohol, is dried by pelleting centrifugation, obtains smart polysaccharide.
embodiment 2
Extract the method for polysaccharide in Fermentation Condition of Monascus spp waste water, step is as follows:
By the product liquid that Fermentation Condition of Monascus spp produces, by squeezing machine, moisture is extruded, obtain Fermentation Condition of Monascus spp waste water, waste water and ethanol are mixed in proportion into the solution that mass concentration is 80% alcohol, refrigerator 0 DEG C of precipitated crystal 28h, centrifugal, by supernatant liquor alcohol precipitation again, twice precipitation merges, dry concentrated, obtains Crude polysaccharides; Described Crude polysaccharides is dissolved in the water by mass volume ratio 1:5, and molecular sieve adsorption is decoloured, centrifugal, gets supernatant liquor and is mixed in proportion into ethanol the solution alcohol precipitation that mass concentration is 80% alcohol, is dried by pelleting centrifugation, obtains smart polysaccharide.
embodiment 3
Extract the method for polysaccharide in Fermentation Condition of Monascus spp waste water, step is as follows:
By the product liquid that Fermentation Condition of Monascus spp produces, by squeezing machine, moisture is extruded, obtain Fermentation Condition of Monascus spp waste water, waste water and ethanol are mixed in proportion into the solution that mass concentration is 77% alcohol, refrigerator 10 DEG C of precipitated crystal 16h, centrifugal, by supernatant liquor alcohol precipitation again, twice precipitation merges, dry concentrated, obtains Crude polysaccharides; Described Crude polysaccharides is dissolved in the water by mass volume ratio 1:5, and molecular sieve adsorption is decoloured, centrifugal, gets supernatant liquor and is mixed in proportion into ethanol the solution alcohol precipitation that mass concentration is 77% alcohol, is dried by pelleting centrifugation, obtains smart polysaccharide.
The smart polysaccharide that the present invention obtains, can be used as medicine or foodstuff additive.
The smart polysaccharide that the present invention obtains, for the preparation of the medicine or the protective foods that prevent cardiovascular and cerebrovascular and tumor disease.
The smart polysaccharide that the present invention obtains, by traditional Chinese medicine production technique, can make tablet, capsule, granule, and electuary etc. for the pharmaceutical dosage form of clinical application, can be applied for the patient clinical suffering from cardiovascular and cerebrovascular diseases.
Claims (9)
1. extract the method for polysaccharide in Fermentation Condition of Monascus spp waste water, step is as follows:
By the product liquid that Fermentation Condition of Monascus spp produces, by squeezing machine, moisture is extruded, obtain Fermentation Condition of Monascus spp waste water, waste water and ethanol are mixed in proportion into the solution that mass concentration is 70%-80% alcohol, refrigerator 0-10 DEG C of precipitated crystal 20-30h, centrifugal, by supernatant liquor alcohol precipitation again, twice precipitation merges, dry concentrated, obtains Crude polysaccharides;
Described Crude polysaccharides is dissolved in the water by mass volume ratio 1:5, and molecular sieve adsorption is decoloured, centrifugal, gets supernatant liquor and is mixed in proportion into ethanol the solution alcohol precipitation that mass concentration is 70%-80% alcohol, is dried by pelleting centrifugation, obtains smart polysaccharide.
2. in Fermentation Condition of Monascus spp waste water according to claim 1, extract the method for polysaccharide, it is characterized in that, described Fermentation Condition of Monascus spp waste water, get rice, peptone raw material monascus through liquid submerged fermentation after 6 to 7 days, the product liquid of the Fermentation Condition of Monascus spp produced, is extruded moisture by squeezing machine, obtains Fermentation Condition of Monascus spp waste water, wherein monascus adopts the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, is numbered the red colouring agent for food, also used as a Chinese medicine bacterial classification of CGMCCNo.6806.
3. extract the method for polysaccharide in Fermentation Condition of Monascus spp waste water according to claim 1, it is characterized in that, described Tc is preferably 0-4 DEG C; Described crystallization time is preferably 20-28h.
4. the smart polysaccharide that the method extracting polysaccharide in Fermentation Condition of Monascus spp waste water as claimed in claim 1 prepares.
5. smart polysaccharide as claimed in claim 4 is as the application of foodstuff additive.
6. smart polysaccharide as claimed in claim 4 is for the preparation of the application of medicine.
7. smart polysaccharide as claimed in claim 4 is for the preparation of prevention cardiovascular and cerebrovascular and the medicine of tumor disease or the application of protective foods.
8. medically acceptable formulation made by smart polysaccharide as claimed in claim 4 and Chinese medicine vehicle.
9. formulation as claimed in claim 8 comprises tablet, capsule, granule, electuary.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110407950A (en) * | 2019-08-28 | 2019-11-05 | 广东工业大学 | A method of mould intracellular polyse is extracted using green solvent |
| CN112094357A (en) * | 2020-09-15 | 2020-12-18 | 淄博职业学院 | Purification method of crude polysaccharide |
| CN112552418A (en) * | 2020-09-15 | 2021-03-26 | 淄博职业学院 | Method for extracting and separating crude polysaccharide from monascus fermentation liquor |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN85103584A (en) * | 1985-05-13 | 1986-10-08 | 宁夏轻工业设计研究所 | The manufacture method of polysaccharide tablets of erythropigment |
| CN101773244A (en) * | 2009-01-13 | 2010-07-14 | 陈小林 | Method for preparing water-soluble functional red yeast rice and product thereof |
| CN102277303A (en) * | 2011-08-08 | 2011-12-14 | 浙江师范大学 | Monascus rubber Mr-99 strain and application thereof |
-
2014
- 2014-09-11 CN CN201410460410.4A patent/CN104371033A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN85103584A (en) * | 1985-05-13 | 1986-10-08 | 宁夏轻工业设计研究所 | The manufacture method of polysaccharide tablets of erythropigment |
| CN101773244A (en) * | 2009-01-13 | 2010-07-14 | 陈小林 | Method for preparing water-soluble functional red yeast rice and product thereof |
| CN102277303A (en) * | 2011-08-08 | 2011-12-14 | 浙江师范大学 | Monascus rubber Mr-99 strain and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 高鑫等: "红曲霉粗多糖的提取及含量测定", 《中国执业药师》, vol. 08, no. 03, 1 March 2011 (2011-03-01), pages 27 - 29 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110407950A (en) * | 2019-08-28 | 2019-11-05 | 广东工业大学 | A method of mould intracellular polyse is extracted using green solvent |
| CN110407950B (en) * | 2019-08-28 | 2021-11-26 | 广东工业大学 | Method for extracting intracellular polysaccharide of mould by using green solvent |
| CN112094357A (en) * | 2020-09-15 | 2020-12-18 | 淄博职业学院 | Purification method of crude polysaccharide |
| CN112552418A (en) * | 2020-09-15 | 2021-03-26 | 淄博职业学院 | Method for extracting and separating crude polysaccharide from monascus fermentation liquor |
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