CN104367612A - Application of alysicarpus vaginalis extract - Google Patents
Application of alysicarpus vaginalis extract Download PDFInfo
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- CN104367612A CN104367612A CN201410667200.2A CN201410667200A CN104367612A CN 104367612 A CN104367612 A CN 104367612A CN 201410667200 A CN201410667200 A CN 201410667200A CN 104367612 A CN104367612 A CN 104367612A
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Abstract
The invention discloses an application of an alysicarpus vaginalis extract in preparation of medicines, foods and healthcare products with a function of protecting liver injuries. Particularly, medicines, foods and healthcare products with the function of protecting acute or chronic liver injuries can be prepared. The alysicarpus vaginalis extract preferably is an ethyl acetate extract, and can be prepared into a plurality of dosage forms by adding pharmaceutical and food related carriers and auxiliary materials. A mice test result shows that the alysicarpus vaginalis extract is capable of obviously lowering CC14 and D-Ga1N and ALT and AST contents of acute liver injury mice serum, and obviously improving liver tissue pathological changes caused by CC14 and D-Ga1N, so as to play a protecting role.
Description
Technical field
The invention belongs to pharmaceutical field, be specifically related to the application of a kind of Canis familiaris L. ant grass extract, be specifically related to Canis familiaris L. ant grass extract and preparing the application in medicine, food and the health product protecting hepatic injury function.
Background technology
Canis familiaris L. ant grass is the herb of leguminous plant alyce clover (Alysicarpus vaginalis (L.) DC.), bean, mountain region bean, artificial flower life, Prunus mume Da Ye Qing, Liao Lan bean, Dan Yecao etc. on another name mountain.Originate in South Asia, East Africa.Be widely distributed in torrid areas, the Eastern Hemisphere.Like tropical moist arid climate.Slightly drought-enduring, acidproof, not resistance to stain.Well-grown on sandy loam, but easy infection nematicide, therefore usually plant on clay.Canis familiaris L. ant grass has many medicinal efficacies, herb: sweet, bitter, flat; Promoting blood circulation to remove obstruction in the collateral, clearing away heat and eliminating dampness, refutes atrophic debility of bones and swells; For traumatic injury, hemiplegia, femur is ached, hepatitis; Venom, fracture, traumatic hemorrhage, skin infection festers and does not close up for a long time.Recording in " China book on Chinese herbal medicine " Dai Nationality's powder stick can Qinghuo Jiedu by following attached side, and promoting the function of the gallbladder to alleviate jaundice, cures mainly " holding together cattle ", i.e. the disease such as the puckery pain of urine heat: Canis familiaris L. ant grass 30g, YELUGUGEN 20g.Decoct soup (Jinghong City name Dai Nationality doctor health Lang Lun proved recipe) for oral administration; Control " hold together case answer strangle ": Canis familiaris L. ant grass 30g, Rhizoma Belamcandae 20g.Decoct soup to take orally (the answering proved recipe of Jinghong City Dai Nationality doctor ripple).
Liver, as parenchymatous organ maximum in human abdominal cavity, is responsible for important physiological function.The tolerance of hepatocyte to anoxia is poor, therefore has the blood supply that Hepatic artery and portal vein provide abundant, and has size bile duct and blood vessel to accompany row to carry bile.It is positioned at the deep of upper right abdomen, has the protection of lower thoracic wall and diaphram.Be subject to easily causing hepatic injury when external environment stimulates or affects at human body: 1, viral infection: caused by multiple hepatitis virus, has infectiousness strong, and route of transmission is complicated, popular wide general, sickness rate high., there is drug induced hepatitis or toxic hepatitis in 2, medicine or chemical toxicant: many medicines and chemical toxicant all can cause hepar damnification.3, indulge in excessive drinking: the infringement of ethanol to liver is very serious, the consequence of infringement comprises alcoholic hepatitis, alcoholic fatty liver, alcoholic cirrhosis, mainly causes because the toxicity of ethanol (ethanol) and metabolite acetaldehyde thereof is directly damaged hepatocyte.4, other reasons: the liver neoplasm of former and secondary, cardiac insufficiency cause liver congestion, some congenital hepatic disease, vein high price nutrition etc., hepatic injury in various degree can be caused, Early manifestation ALT (transaminase) or the bilirubinic rising often of these hepatic injurys, do not dispel the cause of disease, the infringement of liver can increase the weight of further.5, a lot of general infectious disease all can invade liver, as the typhoid fever etc. in Epstein-Barr virus, bacterial infectious disease, can cause rising or other abnormal liver function of serum transaminase.Once easily damaged by violence, intraperitoneal hemorrhage or bile leakage occur, causing bleeding property shock or biliary peritonitis, consequence is serious, must diagnosis in time and correct process.The common drug being applied to hepatic injury patient at present has bifendate, tiopronin, the promise of green spit of fland, occasion gigue, Hai Nuoxin and promise hundred power etc.
Inventor, by New Year research, is found by the experiment of science, and Canis familiaris L. ant grass extract has comparatively outstanding effect in treatment acute liver damage, has also found its mechanism of action for the treatment of simultaneously.
Summary of the invention
The object of this invention is to provide a kind of Canis familiaris L. ant grass extract and prepare the application in medicine, food and the health product protecting hepatic injury function.
The object of the invention is to be achieved through the following technical solutions:
Canis familiaris L. ant grass extract is preparing the application in the medicine of protection hepatic injury function, food and health product.
Described hepatic injury is acute liver damage.
Described Canis familiaris L. ant grass extract extracting method is: water extraction, alcohol extraction, ester is carried, ketone is carried, chromatography, vacuum extraction, supercritical fluid extraction, ultrasonic extraction, Enzymatic Extraction, microwave loss mechanisms.
Described alcohol extraction solvent is methanol, ethanol, n-butyl alcohol; Described ester extraction solvent is ethyl acetate, butyl acetate, ethyl acetate; Ketone extraction solvent is acetone, Ketohexamethylene.
Described Canis familiaris L. ant grass extract is preferably ethyl acetate extract: extracting concrete steps is:
(1) Canis familiaris L. ant grass is cleaned, aeration-drying under 18-25 DEG C of room temperature, shredded and weigh, be placed in opening reactor.
(2) in opening reactor, add 3-5 times of 95% ethanol (v/v) soak 12-24h, then add 95% ethanol (v/v) to medical material 10-15 times amount, carry out reflux, extract.
(3) keep micro-2h that boils, filter, obtain first time extracting solution; After medical material cooling, second time adds 10-15 times amount 95% ethanol (v/v), keeps micro-and boils 1.5 hours, obtains second time filtrate; After medical material cooling, third time adds 10-15 times amount 95% ethanol (v/v), keeps micro-and boils 1 hour, obtains third time filtrate, merges 3 extracting solution, concentrate through Rotary Evaporators, obtain the ethanol extract that relative density is 1.25-1.28.
(4) be placed on water-bath by ethanol extract, be heated to 80-85 DEG C, volatilization, vacuum drying, until extractum weight no longer alleviates, obtains the extractum that relative density is 1.30-1.32, weighs.
(5) add 5-10 times of pure water ethanol extract is all dissolved, add 2-3 times amount petroleum ether again to extract, jolt gently, leave standstill, until there is layering interfaces clearly, draw upper strata petroleum ether solution, repeat petroleum ether extraction process 2-5 time, until petroleum ether layer color is faint yellow and no longer thin out.
(6) by step (5) method, by ethyl acetate, extractum is extracted, acetic acid ethyl acetate extract Rotary Evaporators is concentrated, obtain the Canis familiaris L. ant ethyl oxalacetate extractum that relative density is 1.22-1.24, ethyl acetate extract is placed on water-bath, be heated to 80-85 DEG C, solvent flashing is also transferred to vacuum drying oven continuation drying, obtains the Canis familiaris L. ant ethyl oxalacetate extract that relative density is 1.28-1.30.
The medicine of protection hepatic injury function, by with Canis familiaris L. ant grass extract for principle active component and pharmaceutically acceptable adjuvant form, the percentage by weight of Canis familiaris L. ant grass extract in pharmaceutical formulation is 0.1-99%.Be processed as multiple dosage form, can be pill, granule, liquor, injection, capsule, tablet simultaneously.Described adjuvant can be: mannitol, sorbitol, sorbic acid or potassium salt, sodium pyrosulfite, sodium sulfite, sodium thiosulfate, cysteine hydrochloride, TGA, methionine, vitamin A, vitamin C, vitamin E, vitamin D, azone, EDETATE SODIUM, Ethylenediaminetetraacetic Acid Calcium Salt, sodium lactate, xylitol, maltose, glucose, fructose, dextran, glycine, starch, sucrose, lactose, mannitol, cellulose and its derivates, alginate, gelatin, polyvinylpyrrolidone, glycerol, propylene glycol, ethanol, Cera Flava, lanoline, liquid paraffin, hexadecanol, gallate ester, agar, triethanolamine, basic amino acid, Polyethylene Glycol, cyclodextrin, beta-schardinger dextrin-, phospholipid material, Kaolin, Pulvis Talci, calcium stearate, magnesium stearate, soybean oil, olive oil, Oleum Camelliae, Petiolus Trachycarpi oil, Radix Angelicae Sinensis oil, Oleum Hippophae, Oleum Curcumae, Rhizoma Chuanxiong oil, pearl barley oil, safflower oil, Fructus Zanthoxyli oil, Oleum Bulbus Allii, Ovum Gallus domesticus Flavus lecithin, soybean phospholipid, hydrogenated yolk lecithin, hydrogenated soya phosphatide, synthetic phospholipid, oleic acid or oleate, vitamin E, sodium sulfite, sodium thiosulfate, vitamin C etc.
The food of protection hepatic injury function and health product, by with Canis familiaris L. ant grass extract for principle active component and food additive, raw-food material form, the percentage by weight of Canis familiaris L. ant grass extract in formula is 1-50%.Liquor, tablet, granule, capsule can be processed as or be processed as coarse grain, cake, beverage as food adjuvant.Being processed as food and health product is because inventor finds: Canis familiaris L. ant grass extract not only can directly act on hepatic injury position as medicine and play protected effect; can also as food or health product by clothes for patients with after carry out conditioning health, the physical recovery for hepatic injury patient also can play good result.
Beneficial effect of the present invention: Canis familiaris L. ant grass extract of the present invention has good therapeutic effect to acute and chronic liver injury; simultaneously Canis familiaris L. ant grass extract is as food and health product application aspect; also good recovery effects is had for hepatic injury patient; this illustrates that Canis familiaris L. ant of the present invention grass extract has the effect of protection hepatic injury preferably; can be used for preparing the medicine of protection hepatic injury function, food and health product, is a kind of novelty teabag of application and development of Canis familiaris L. ant grass extract.
Detailed description of the invention
the preparation of embodiment one Canis familiaris L. ant ethyl oxalacetate extract
concrete steps are:
(1) Canis familiaris L. ant grass is cleaned, aeration-drying under 18-25 DEG C of room temperature, shredded and weigh, be placed in opening reactor;
(2) in opening reactor, add 3-5 times of 95% ethanol (v/v) soak 12-24h, then add 95% ethanol (v/v) to medical material 10-15 times amount, carry out reflux, extract;
(3) keep micro-2h that boils, filter, obtain first time extracting solution; After medical material cooling, second time adds 10-15 times amount 95% ethanol (v/v), keeps micro-and boils 1.5 hours, obtains second time filtrate; After medical material cooling, third time adds 10-15 times amount 95% ethanol (v/v), keeps micro-and boils 1 hour, obtains third time filtrate, merges 3 extracting solution, concentrate through Rotary Evaporators, obtain the ethanol extract that relative density is 1.25-1.28;
(4) be placed on water-bath by ethanol extract, be heated to 80-85 DEG C, volatilization, vacuum drying, until extractum weight no longer alleviates, obtains the extractum that relative density is 1.30-1.32, weighs;
(5) add 5-10 times of pure water ethanol extract is all dissolved, add 2-3 times amount petroleum ether again to extract, jolt gently, leave standstill, until there is layering interfaces clearly, draw upper strata petroleum ether solution, repeat petroleum ether extraction process 2-5 time, until petroleum ether layer color is faint yellow and no longer thin out;
(6) by step (5) method, by ethyl acetate, extractum is extracted, acetic acid ethyl acetate extract Rotary Evaporators is concentrated, obtain the Canis familiaris L. ant ethyl oxalacetate extractum that relative density is 1.22-1.24, ethyl acetate extract is placed on water-bath, be heated to 80-85 DEG C, solvent flashing is also transferred to vacuum drying oven continuation drying, obtains the Canis familiaris L. ant ethyl oxalacetate extract that relative density is 1.28-1.30.
embodiment two Canis familiaris L. ant grass extract is processed as food and health product
1 by Canis familiaris L. ant grass ethanol extraction vacuum drying, and after adding starch, glucose, dietary fiber, fully stir, coarse pulverization is to 60-80 order, and vacuum packaging, by 10g/ bag subpackage, obtains its instant powder.
2., by Canis familiaris L. ant grass ethanol extraction extractum, ethanol evaporation is to content lower than 0.1% again, adds dietary fiber, malted milk, xylitol, flour, is crushed to 60-80 order in pulverizer, add 5-10% pure water, extruder grain, dry, sieve, packaging, obtains its granule.
3. by Canis familiaris L. ant ethyl oxalacetate extract extractum, add pure water stirring and dissolving, add antiseptic, Mel, red bean juice, fill after sterilizing, sterilization, obtains its liquor.
4., by extract obtained for Canis familiaris L. ant grass Herb supercritical fluid extraction, after oven dry, add in coarse grain goods as adjuvant, addition is 20%, finally makes easily digested coarse grain goods.
5. by extract obtained for Canis familiaris L. ant grass Herb vacuum extraction, after oven dry, add in sodium bicarbonate cookies raw material as adjuvant, Canis familiaris L. ant grass soda cracker is made in polish, and its extract addition is 5%.
6. by Canis familiaris L. ant ethyl oxalacetate extract solvent flashing again, must not higher than 0.01% to solvent, add Mel, vitamin E, vitamin B1, folic acid, Ferrous gluconate, zinc gluconate, soybean oil and Cera Flava, make health product capsule, every containing Canis familiaris L. ant grass extract 50mg, every heavy 500mg.
7. after being dried by Canis familiaris L. ant grass sonicated extract, add sodium selenite, Cera Flava, glycerol, gelatin, iron oxide brown, make health product capsule, every containing Canis familiaris L. ant grass extract 80mg, the heavy 500mg of every capsules.
effect example
1 Canis familiaris L. ant ethyl oxalacetate extract is to CCl
4
with the protective effect of D-DalN induced mice acute liver damage
1.1 experiment material
1.1.1 laboratory animal
Kunming mouse 60, male and female half and half, body weight 20 ± 2g, is provided by Guangxi University of Chinese Medicine's Experimental Animal Center, the quality certification number, dynamic No. 11004th, the word of osmanthus doctor.
1.1.2 experiment reagent
Bifendate drop pill: 250 balls/bottle Zhejiang Wanbang Pharmaceutical Co., Ltd., lot number: A02140101
Carbon tetrachloride (CCL
4): (analytical pure 500ml/ bottle) Xi Long chemical plant, Guangdong product, lot number: 20111130;
D-galactosamine hydrochlorate (D-GalNHCL): Sigma Batch No:080612
Glutamate pyruvate transaminase (ALT) testing cassete, reagent company limited is built up in Nanjing, lot number: 20140410
Glutamic oxaloacetic transaminase, GOT (AST) testing cassete, reagent company limited is built up in Nanjing, lot number: 20140303
1.1.3 experimental apparatus
| Water-bath | Guo Hua Electrical Appliances Co., Ltd model: HH-2 |
| Microplate reader | U.S. BIO-TEK |
| Micro sample adding appliance | France GILSON |
| Centrifuge | Thermo Fisher Sorval ST 16 |
| Ten thousand/gram electronic balance | Germany Sai Duolisi model: BP211D |
| Ultra-pure water instrument | France Merck Mi Libo model: bright and limpid-D 24 UV |
1.1.4 medicinal liquid configuration
Canis familiaris L. ant natural plant height dosage medicinal liquid: get 0.3036g extractum (every 1g extractum is equivalent to 197.6g crude drug) and add ultra-pure water dissolving, be settled to 30mL;
Dosage medicinal liquid in Canis familiaris L. ant grass: dosage is 20g/kg, gets 0.1518g extractum (every 1g extractum is equivalent to 197.6g crude drug) and adds ultra-pure water dissolving, be settled to 30mL;
Canis familiaris L. ant grass low dosage medicinal liquid: dosage is 10g/kg, gets 0.0759g extractum (every 1g extractum is equivalent to 197.6g crude drug) and adds ultra-pure water dissolving, be settled to 30mL;
Benzene dibasic acid esters drop pill 25 ball (every ball is containing bifendate 1.5mg) grind into powder, adds water and is made into suspension, be settled to 37.5mL.
1.2 experimental technique
1.2.1 Canis familiaris L. ant oxalacetic acid second extract is to CCl
4
the impact of induced mice acute liver damage
Get Kunming mouse 60, male and female half and half, body weight 20 ± 2.0g, random packet, be respectively blank group, model group, bifendate group (0.2g/kg), Canis familiaris L. ant ethyl oxalacetate extract high dose group (suitable 40g/kg crude drug), middle dosage group (suitable 20g/kg crude drug), low dose group (suitable 10g/kg crude drug), often organize 10.Blank group and model group give pure water gavage, and all the other each group gives corresponding medicine gavage, and administration volume is 0.2mL/10g, 1 time/d, continuous 10d, and after last administration, except blank group, all the other respectively organize lumbar injection 0.15%CCL
4peanut oil solution 0.1 mL/10g, fasting 12h, freely drinks water, and plucks eyeball and gets blood, puts to death, and taking-up hepatomegaly leaf is put in 10% formalin and preserved, for hepatic tissue pathology cut sections for microscopic examination; Treat that serum is separated out, centrifugalize serum, measure ALT, AST content by test kit description.
1.2.2 Canis familiaris L. ant ethyl oxalacetate extract is on the impact of D-DalN induced mice acute liver damage
Get Kunming mouse 60, male and female half and half, body weight 20 ± 2.0g, random packet, be respectively blank group, model group, bifendate group (0.2g/kg) Canis familiaris L. ant ethyl oxalacetate extract high dose group (suitable 40g/kg crude drug), middle dosage group (suitable 20g/kg crude drug), low dose group (suitable 10g/kg crude drug), often organize 10.Blank group and model group give pure water gavage, and all the other each group gives relative medicine gavage, 0.2mL/10g, 1 time/d, continuous 10d, and blank group normal saline 0.1ml/10g after last administration, all the other respectively organize lumbar injection D-GalN 500mg/kg.Fasting 12h, freely drinks water, and plucks eyeball and gets blood, puts to death, and taking-up hepatomegaly leaf is put in 10% formalin and preserved, for hepatic tissue pathology cut sections for microscopic examination; Treat that serum is separated out, centrifugalize serum, measure ALT, AST content by test kit description.
1.3 experimental result
1.3.1 Canis familiaris L. ant ethyl oxalacetate extract is to CCL
4
the impact of induced mice acute liver damage
Experimental result is as shown in table 1, and Canis familiaris L. ant grass high dose group, middle dosage group, low dose group all obviously can reduce mice serum ALT and AST content
The display of hepatic tissue HE dyeing pathologic finding is blank, and under matched group microscope, lobules of liver clear in structure is complete, and hepatocyte radially arranges around central vein, and portal area structure is normal, does not occur hepatocellular degeneration, necrosis and inflammatory cell infiltration; The structural deterioration of model group lobules of liver is serious, can't see liver rope sinus hepaticus structure clearly, occurs that severe hepatocellular balloon sample becomes and with inflammatory cell infiltration around centre vein and portal area; Bifendate group positive group lobules of liver and portal area structural integrity, there is a small amount of ballooning degeneration of liver cells in limiting plate district; , around portal area, there is a small amount of ballooning degeneration of liver cells in ethyl acetate high dose group lobules of liver portal area structural integrity; Dosage group in ethyl acetate: lobules of liver structure is slightly disorderly, a small amount of limiting plate district hepatocyte generation balloon sample becomes; Ethyl acetate low dose group lobules of liver structure disturbance, there is ballooning degeneration of liver cells in lobules of liver edge, overall lesion degree comparatively model group is light.
Table 1 Canis familiaris L. ant ethyl oxalacetate extract is to CCL
4the impact of induced Acute hepatic injury mice serum ALT, AST content (
± s, n=10)
| Group | Dosage (g/kg) | ALT(Ka Menshi unit) | AST(Ka Menshi unit) |
| Blank group | - | 30.9±3.6 ** | 38.6±3.8 ** |
| Model group | - | 591.5±63.3 | 174.7±16.0 |
| Bifendate group | 0.2 | 485.0±44.2 ** | 123.8±17.1 ** |
| Canis familiaris L. ant grass high dose group | 40 | 523.9±50.9 * | 141.8±14.1 ** |
| Dosage group in Canis familiaris L. ant grass | 20 | 519.6±56.0 * | 141.9±18.8 ** |
| Canis familiaris L. ant grass low dose group | 10 | 533.5±50.4 * | 152.9±17.8 * |
Note: compare with model group,
*p<0.05,
*p<0.01.
1.3.2 Canis familiaris L. ant ethyl oxalacetate extract is on the impact of D-GalN induced mice acute liver damage
Experimental result is as shown in table 2, and Canis familiaris L. ant ethyl oxalacetate extract high dose group, middle dosage group and low dose group all significantly can reduce mice serum ALT and AST content.
The display of hepatic tissue HE dyeing pathologic finding, under blank group microscope, lobules of liver clear in structure is complete, and hepatocyte radially arranges around central vein, and portal area structure is normal, does not occur hepatocellular degeneration, necrosis and inflammatory cell infiltration; Model group lobules of liver structure is seriously damaged, hepatocyte irregular arrangement, and hepatocyte occurs filling the air blister degeneration, and with inflammatory cell infiltration, obvious inflammatory cell infiltration appears in portal area; Bifendate group positive group lobules of liver, portal area structure are normal, and point-like inflammatory cell infiltration appears in respective regions; Ethyl acetate high dose group lobules of liver structure is still clear, and hepatocyte occurs mild swelling individually; In ethyl acetate, dosage group lobules of liver structure is normal, and point-like inflammatory cell infiltration appears in central vein and portal area, and mild swelling appears in hepatocyte; Ethyl acetate low dose group lobules of liver structure goes to pot, and obvious tumefaction appears in hepatocyte, and arrange irregular, inflammatory cell infiltration appears in portal area, and overall lesion degree comparatively model group is light.
Table 2 Canis familiaris L. ant ethyl oxalacetate extract on the impact of D-GalN induced Acute hepatic injury mice ALT, AST content (
± s, n=10)
| Group | Dosage (g/kg) | ALT (Ka Menshi unit) | AST (Ka Menshi unit) |
| Blank group | - | 9.6±1.2 ** | 20.3±2.1 ** |
| Model group | - | 227.2±28.9 | 131.8±16.3 |
| Bifendate group | 0.2 | 81.8±10.7 ** | 91.2±8.9 ** |
| Canis familiaris L. ant grass high dose group | 40 | 115.1±15.8 ** | 95.5±12.6 ** |
| Dosage group in Canis familiaris L. ant grass | 20 | 112.9±14.8 ** | 93.4±11.8 ** |
| Canis familiaris L. ant grass low dose group | 10 | 197.4±23.9 * | 111.6±13.4 * |
Note: compare with model group,
*p<0.05,
*p<0.01
1.3 experiment conclusion
In serum, AST, ALT content is the critical biochemical index of reaction hepar damnification, and Canis familiaris L. ant ethyl oxalacetate extract can reduce CCL
4damage mice with D-GalN and induced Acute liver and hinder Serum ALT, AST content, and obviously can improve CCL
4change with the hepatic tissue pathology that D-GalN causes, illustrate that Canis familiaris L. ant ethyl oxalacetate extract is to CCL
4protective effect is had with D-GalN induced mice acute liver damage.
Claims (9)
1. Canis familiaris L. ant grass extract is preparing the application in the medicine of protection acute liver damage function, food and health product.
2. application according to claim 1, is characterized in that: described hepatic injury is acute liver damage.
3. application according to claim 1, is characterized in that: described Canis familiaris L. ant grass extract extracting method is: water extraction, alcohol extraction, ester is carried, ketone is carried, chromatography, vacuum extraction, supercritical fluid extraction, ultrasonic extraction, Enzymatic Extraction, microwave loss mechanisms.
4. application according to claim 3, is characterized in that: described alcohol extraction solvent is methanol, ethanol, n-butyl alcohol; Described ester extraction solvent is ethyl acetate, butyl acetate, ethyl acetate; Ketone extraction solvent is acetone, Ketohexamethylene.
5. application according to claim 3, it is characterized in that: described Canis familiaris L. ant grass extract is ethyl acetate extract: extracting concrete steps is: first by after Canis familiaris L. ant grass drying and crushing, after 95% soak with ethanol, carry out reflux, extract, keep micro-2h that boils, filter, obtain first time extracting solution, after medical material cooling, then add 12 times amount 95% ethanol, keep micro-to boil 1.5 hours, obtain second time filtrate;
Medical material cools slightly, adds 12 times amount 95% ethanol, keeps micro-and boils 1 hour, obtains third time filtrate, merges 3 extracting solution, and Rotary Evaporators concentrates, first obtained 95% ethanol extract; Dry rear water dissolution, then extracts by petroleum ether, chloroform, ethyl acetate successively, through concentrating, after solvent flashing and vacuum drying, to obtain final product.
6. protect a medicine for hepatic injury function, it is characterized in that: form for principle active component and pharmaceutically acceptable adjuvant by with Canis familiaris L. ant grass extract, the percentage by weight of Canis familiaris L. ant grass extract in pharmaceutical formulation is 0.1-99%.
7. the medicine of protection hepatic injury function according to claim 6, is characterized in that: can be processed as pill, granule, liquor, injection, capsule, tablet.
8. protect food and the health product of hepatic injury function, it is characterized in that: form for principle active component and food additive, raw-food material by with Canis familiaris L. ant grass extract, the percentage by weight of Canis familiaris L. ant grass extract in formula is 1-50%.
9. the food of protection hepatic injury function according to claim 8 and health product, is characterized in that: can be processed as liquor, tablet, granule, capsule or be processed as coarse grain, cake, beverage as food adjuvant.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106135881A (en) * | 2015-03-20 | 2016-11-23 | 苏会静 | A kind of Semen Coicis juice |
| CN109498603A (en) * | 2018-12-28 | 2019-03-22 | 广西中医药大学 | A kind of construction method of carbon tetrachloride macaca fascicularis acute liver damage animal model |
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2014
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Non-Patent Citations (1)
| Title |
|---|
| 南京中医药大学编著: "《中药大辞典2版（修订本）》", 30 June 2014, 上海科学技术出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106135881A (en) * | 2015-03-20 | 2016-11-23 | 苏会静 | A kind of Semen Coicis juice |
| CN109498603A (en) * | 2018-12-28 | 2019-03-22 | 广西中医药大学 | A kind of construction method of carbon tetrachloride macaca fascicularis acute liver damage animal model |
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