CN104357370A - Streptomyces albidus strain capable of producing molluscicidal active substance and application of streptomyces albidus strain - Google Patents

Streptomyces albidus strain capable of producing molluscicidal active substance and application of streptomyces albidus strain Download PDF

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Publication number
CN104357370A
CN104357370A CN201410726649.1A CN201410726649A CN104357370A CN 104357370 A CN104357370 A CN 104357370A CN 201410726649 A CN201410726649 A CN 201410726649A CN 104357370 A CN104357370 A CN 104357370A
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strain
streptomyces
streptomyces albidus
cgmcc
producing
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邢云天
戴建荣
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Jiangsu Institute of Parasitic Diseases
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Jiangsu Institute of Parasitic Diseases
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/465—Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses a streptomyces albidus strain capable of producing a molluscicidal active substance and application of the streptomyces albidus strain, and belongs to the technical field of parasite prevention and control. The collection number of the streptomyces albidus strain belonging to the actinomycetes is CGMCC No. 9785, and the sequence of the 16SrDNA is as shown in SEQ ID No.1. The invention also discloses application of a streptomyces albidus strain fermentation product in preparation of molluscicidal medicines. The streptomyces albidus strain capable of producing the molluscicidal active substance has the advantages that the actinomycete CGMCC No. 9785 is a strain of streptomyce new specie, and the fermentation liquor of the strain has excellent killing effect on oncomelania; besides, the streptomyces albidus strain has high development prospect.

Description

One strain produces micro-streptomyces albus and the application thereof of ovicidal trait material
Technical field
The present invention relates to a strain produce ovicidal trait material streptomyces ( streptomyces albidus) actinomycetes, belong to parasite control technical field.
Background technology
Schistosomiasis japanica is the great communicable disease of the healthy and life security of a kind of serious harm our people, through preventing and treating for many years, achieve achievement highly visible, but schistosomiasis propagation and trend's elements still exist, become and cause schistosomiasis epidemic repeatedly or potential threat factor popular again.Oncomelania hupensis is unique intermediate host of schistosomiasis japanica, and Killing Oncomelania Hupensis is prevention and corntrol schistosomiasis endemic, one of the Important Action consolidating the achievement that prevention and cure of snail fever obtains.Therefore find new efficient, low toxicity, inexpensive molluscacide and be still current urgent and important task.
The development of current biogenic pesticide receives the concern of countries in the world, and existing multiple biological pesticide product obtains widespread use, comprising jingganmycin, Plant hormones regulators,gibberellins, Avrmectin, kasugamycin etc.From actinomycetes, screen novel agrochemical is one of focus of current research.
In soil, the microorganism of enormous amount is multiplied in existence, in order to adapt to ecotope, microorganism in soil defines unique physiological property and metabolic characteristic, and then creates diversified secondary metabolite, becomes the mankind and finds and develop the important source with bioactive compound.
Summary of the invention
The technology that the present invention will solve is to provide the streptomycete that a strain can produce ovicidal trait material.
For achieving the above object, the present invention is by the following technical solutions:
One strain produce ovicidal trait material streptomyces ( streptomyces albidus) actinomycetes, its preserving number is CGMCC No.9785.
Actinomycetes CGMCC No.9785 obtains by being separated in Tonghua, Jilin Province city Yuhuang Mount soil.
The sequence of described actinomycetes CGMCC No.9785, its 16S rDNA is as shown in SEQ ID No.1.
The application of described actinomycetes CGMCC No.9785, this bacterium tunning is preparing the purposes in molluscacide.
Actinomycetes have following microorganism feature:
(1) cultural characteristic and morphological specificity
Described bacterial strain is well-grown on potato dextrose agar (PDA), Gause I synthetic medium, glycerine asparagine nutrient agar (ISP2), inorganic salt starch agar medium (ISP3), yeastex Fructus Hordei Germinatus asccharin nutrient agar (ISP4), oatmeal nutrient agar (ISP5), Czapek substratum, all can produce more rich aerial hyphae, but all produce without soluble pigment.The cultural characteristic of described bacterial strain on different culture media refers to table 1.Observing described bacterial strain fibrillae of spores is under an optical microscope curve shape, spore ovalize.
The cultural characteristic of table 1 bacterial strain CGMCC No.9785
(2) physiological and biochemical property
Described bacterial strain can utilize glucose, fructose, sucrose, pectinose, maltose; Seminose, N.F,USP MANNITOL, lactose, wood sugar can not be utilized.Mierocrystalline cellulose can be utilized, without melanochrome and H 2s produces.Can make gelatine liquefication, milk solidifies and peptonizes.
(3) described actinomycetes, the sequence of its 16S rDNA is for shown in SEQ ID No.1.
Beneficial effect of the present invention: described actinomycetes CGMCC No.9785 is that a streptomycete belongs to novel species, and its fermented liquid has good killing action to oncomelania, has higher DEVELOPMENT PROSPECT.
Biological material specimens preservation: a strain produce ovicidal trait material streptomyces ( streptomyces albidus) actinomycetes, be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, be called for short CGMCC, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, deposit number CGMCC No.9785, preservation date on October 16th, 2014.
Accompanying drawing explanation
The cultivation form that Fig. 1 bacterial strain of the present invention (is cultivated 10 days) in Gause I substratum.
The mycelia of Fig. 2 bacterial strain of the present invention and fibrillae of spores form, 400 ×.
Embodiment
Below in conjunction with specific embodiment, the present invention is further illustrated
Embodiment 1: the separation of bacterial strain CGMCC No.9785
Take in the triangular flask that 1g soil sample joins containing 10mL physiological saline, fully mix.10 times of serial dilutions are done to sample, respectively gets 100 μ L 10 -1, 10 -2, 10 -3, 10 -4, 10 -5dilution bacterium liquid is coated on Gause I substratum, is inverted for 28 DEG C and cultivates.The bacterium colony that in good time picking form, color are different carries out line purifying.For the actinomycetes of purifying, according to colonial morphology feature, get rid of identical bacterial strain, proceed to the preservation of 4 DEG C, inclined-plane, numbering, give over to bacterial strain screening and use.
Embodiment 2: the cultivation of bacterial strain CGMCC No.9785
(1) slant culture: cultivate 7 days.Substratum: Zulkovsky starch 2%, saltpetre 0.1%, sodium-chlor 0.05%, dipotassium hydrogen phosphate 0.05%, magnesium sulfate 0.05%, ferrous sulfate 0.01%, agar 1.5%.Distilled water is prepared, initial pH 7.2-7.4.
(2) fermentation culture: from the single bacterium colony access fermention medium that inclined-plane picking is activated, 28 DEG C, 180r/min shake-flask culture 7 days.Substratum: glucose 2%, Zulkovsky starch 2%, saltpetre 0.5%, dipotassium hydrogen phosphate 0.05%.With distilled water preparation, initial pH 7.2-7.4.
Embodiment 3 bacterial strain CGMCC No.9785 fermented liquid molluscicidal effect
By fermented liquid 0.22 μm of filtering with microporous membrane, filtrate is diluted to 1/10,1/50 and 1/100 of fermenation raw liquid respectively, each concentration is put into 10 oncomelanias and is become spiral shell, often organizing amount of liquid medicine is 100mL, liquid is removed after soaking oncomelania 72h, clear water rinses, recovery 48h, and hammering method is observed oncomelania life or death and counted; Each concentration is all done three groups of parallel clear water of simultaneously establishing and is contrasted.1/10,1/50 and 1/100 mortality of snails of fermenation raw liquid concentration is respectively 100.00%, 100.00% and 86.66%, shows that this fermented liquid has good molluscicidal effect.
<210> SEQ ID NO: 1
<211> 1415
<212> DNA
<213> streptomyces (Streptomyces albidus) actinomycetes CGMCC No.9785
 
<400>1
CACATGCAAG TCGAACGATG AAGCCTTTCG GGGTGGATTA GTGGCGAACG GGTGAGTAAC 60
ACGTGGGCAA TCTGCCCTTC ACTCTGGGAC AAGCCCTGGA AACGGGGTCT AATACCGGAT 120
AACACTCTGT CCGCATGGGA CGGGGTTAAA AGCTCCGGCG GTGAAGGAGA GCCCGCGGCC 180
TATCAGCTTG TTGGTGGGGT AATGGCCTAC CAAGGCGACG ACGGGTAGCC GGCCTGAGAG 240
GGCGACCGCC ACACTGGGAC TGAGACACGG CCCAGACTCC TACGGGAGGC AGCAGTGGGG 300
AATATTGCAC AATGGGCGAA AGCCTGATGC AGCGACGCCG CGTGAGGGAT GACGGCCTTC 360
GGGTTGTAAA CCTCTTTCAG CAGGGAAGAA GCGAAAGTGA CGGTACCTGC AGAAGAAGCG 420
CCGGCTAACT ACGTGCCAGC AGCCGCGGTA ATACGTAGGG CGCAAGCGTT GTCCGGAATT 480
ATTGGGCGTA AAGAGCTCGT AGGCGGTTGT CACGTCGGAT GTGAAAGCCC GGGGCTTAAC 540
CCCGGGTCTG CATTCGATAC GGGCTAGCTA GAGTGTGGTA GGGGAGATCG GAATTCCTGG 600
TGTAGCGGTG AAATGCGCAG ATATCAGGAG GAACACCGGT GGCGAAGGCG GATCTCTGGG 660
CCATTACTGA CGCTGAGGAG CGAAAGCGTG GGGAGCGAAC AGGATTAGAT ACCCTGGTAG 720
TCCACGCCGT AAACGTTGGG AACTAGGTGT TGGCGACATT CCACGTCGTC GGTGCCGCAG 780
CTAACGCATT AAGTTCCCCG CCTGGGGAGT ACGGCCGCAA GGCTAAAACT CAAAGGAATT 840
GACGGGGGCC CGCACAAGCA GCGGAGCATG TGGCTTAATT CGACGCAACG CGAAGAACCT 900
TACCAAGGCT TGACATATAC CGGAAAGCAT CAGAGATGGT GCCCCCCTTG TGGTCGGTAT 960
ACAGGTGGTG CATGGCTGTC GTCAGCTCGT GTCGTGAGAT GTTGGGTTAA GTCCCGCAAC 1020
GAGCGCAACC CTTGTTCTGT GTTGCCAGCA TGCCCTTCGG GGTGATGGGG ACTCACAGGA 1080
GACTGCCGGG GTCAACTCGG AGGAAGGTGG GGACGACGTC AAGTCATCAT GCCCCTTATG 1140
TCTTGGGCTG CACACGTGCT ACAATGGCCG GTACAATGAG CTGCGATGCC GCGAGGCGGA 1200
GCGAATCTCA AAAAGCCGGT CTCAGTTCGG ATTGGGGTCT GCAACTCGAC CCCATGAAGT 1260
CGGAGTTGCT AGTAATCGCA GATCAGCATT GCTGCGGTGA ATACGTTCCC GGGCCTTGTA 1320
CACACCGCCC GTCACGTCAC GAAAGTCGGT AACACCCGAA GCCGGTGGCC CAACCCCTTG 1380
TGGGAGGGAG CTGTCGAAGG TGGGACTGGC GATTG 1415
 

Claims (3)

1. a strain produce ovicidal trait material streptomyces ( streptomyces albidus) actinomycetes, its preserving number is CGMCC No.9785.
2. actinomycetes CGMCC No.9785 according to claim 1, it is characterized in that, the sequence of its 16S rDNA is as shown in SEQ ID No.1.
3. the application of actinomycetes CGMCC No.9785 described in claim 1, is characterized in that this bacterium tunning is preparing the purposes in molluscacide.
CN201410726649.1A 2014-12-04 2014-12-04 Streptomyces albidus strain capable of producing molluscicidal active substance and application of streptomyces albidus strain Pending CN104357370A (en)

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Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
向阳等: "土味链霉菌339菌株杀螺活性物的提取", 《华中师范大学学报(自然科学版)》 *
孙启祥，彭镇华: "抑螺防病林建设中生物材料的筛选及抑螺机理研究进展", 《湿地科学与管理》 *

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Application publication date: 20150218