CN104356398B - Method for extracting gutta-percha by using full-biological enzyme method - Google Patents
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Abstract
A method for extracting gutta-percha by a full biological enzyme method comprises the steps of firstly blasting and cracking the eucommia bark serving as a raw material, then sequentially using protease to decompose protein components in the eucommia bark, using pectinase to decompose cell walls and intercellular layers in the eucommia bark, using cellulase to improve the decomposition of cellulose and hemicellulose, so as to decompose non-glue substances in the eucommia bark into components which can be completely separated from the gutta-percha, separating a target object from waste components by using a method of rotating a separating cylinder in an enzymolysis device in a water tank, arranging a bent plate stirring blade with an angular cross section on the inner wall of the separating cylinder, enabling the included angle of the bent plate to be not less than 120 degrees, enabling the stirring blade to form an inclination angle of 10-15 degrees with a support shaft, and staggering elongated holes on the bent plate, ensuring the thoroughness of separation of the target object and the waste components, and being clean, safe and environment-, low cost and high efficiency, and the purity of the extracted gutta-percha is high and can reach more than 92 percent, thus being suitable for industrialized production.
Description
Technical Field
The invention relates to a method for extracting natural gum, in particular to a method for extracting gutta-percha by a full-biological enzyme method.
Background
The eucommia ulmoides tree belongs to a unique precious tree species in China, and the eucommia ulmoides gum contained in the eucommia ulmoides tree is used as natural rubber, has the advantages of high insulativity and corrosion resistance, is a high-quality material for manufacturing submarine cables, and is a good raw material for manufacturing electrical appliance insulating materials, strong medicine containers and tooth filling. The gutta-percha can also be used for manufacturing medical splints and automobile tires, and producing shape memory materials, temperature detection materials, modified plastics and other rubber products, thereby having wide application prospect.
The existing methods for extracting gutta-percha from eucommia trees are divided into two types, wherein one type is as follows: the raw material eucommia bark is subjected to full fermentation treatment, then is subjected to cooking treatment by using a strong alkaline aqueous solution, and then is separated by using a mechanical cleaning method. The method not only has serious environmental pollution and low efficiency, but also has certain damage to the eucommia ulmoides adhesive tape, the purity of the extracted eucommia ulmoides adhesive tape is also low, and the production cost is higher as a result.
Another method for extracting gutta-percha is to pretreat raw materials such as bark of eucommia ulmoides with compound biological enzyme, and further dissolve the raw materials with organic solvents such as petroleum ether to obtain gutta-percha.
In summary, no safe and efficient gutta percha extraction method and related equipment which can be applied to industrial production exist in the prior art.
Disclosure of Invention
In order to solve the technical problems, the invention provides the method for extracting the gutta-percha by the full-biological enzyme method, which has the advantages of simple use equipment, safe and environment-friendly production process and high production efficiency, and is suitable for industrial production.
The technical scheme adopted by the invention to solve the technical problems is as follows: a method for extracting gutta-percha by a whole biological enzyme method comprises the following steps:
1) firstly, cutting eucommia bark serving as a raw material into pieces with the area not more than 25cm by using a hay cutter2Soaking the small blocks in clean water at normal temperature for more than 12h, taking out, and blasting in a blasting machine to obtain cortex Eucommiae intermediate I;
in the step, the blasting temperature is controlled below 50 ℃, the blasting pressure is controlled between 1.4MPa and 1.8MPa, and the blasting time is controlled within 0.5S;
2) putting the eucommia bark intermediate I obtained in the step 1) into a protease aqueous solution, repeatedly stirring and washing for 6-7h, then repeatedly stirring and washing for 1.5-2.5h with clear water to obtain an eucommia bark intermediate II:
when washing in the step, various components are prepared according to the following parts by weight: 90-110 parts of eucommia bark intermediate I, 160 parts of hot water 140 and 10-15 parts of protease, wherein the temperature of the hot water is controlled to be 40-50 ℃;
3) putting the eucommia bark intermediate II obtained in the step 2 into aqueous solution of pectinase, repeatedly stirring and washing for 6-7h, then repeatedly stirring and washing for 1.5-2.5h with clear water to obtain an eucommia bark intermediate III:
when washing in the step, various components are prepared according to the following parts by weight: 90-110 parts of eucommia bark intermediate II, 160 parts of hot water 140 and 10-15 parts of pectinase, wherein the temperature of the hot water is controlled to be 40-50 ℃;
4) putting the eucommia bark intermediate III obtained in the step 3 into a water solution of cellulase, repeatedly stirring and washing for 6-7h, and then repeatedly stirring and washing with clear water for 1.5-2.5h to obtain white filamentous eucommia ulmoides gum;
when washing in the step, various components are prepared according to the following parts by weight: 90-110 parts of eucommia bark intermediate III, 160 parts of hot water 140 and 10-15 parts of cellulase, wherein the temperature of the hot water is controlled to be 40-50 ℃;
the steps 2), 3) and 4) are all carried out in the same enzymolysis device, the enzymolysis device is provided with a water tank and a separating cylinder, the separating cylinder is cylindrical and is horizontally arranged on the upper edge of the water tank through a central supporting shaft, the water tank is filled with the water solution of the corresponding enzyme, one part of the separating cylinder is immersed in the water solution of the corresponding enzyme, the inside of the separating cylinder is provided with the corresponding eucommia bark intermediate, the side wall of the separating cylinder is provided with a separating hole with the diameter of 0.8-1.2mm, the inner wall of the separating cylinder is also longitudinally provided with a stirring blade, the stirring blade is a bent plate with the cross section being angular, the included angle of the bent plate is not less than 120 degrees, the length direction of the bent plate and the central supporting shaft have the inclined angle, the inclined angle is 10-15 degrees, and the opening of the included angle of the bent plate is opposite to the rotating direction of the separating cylinder, and the bending plate is provided with elongated holes in a staggered manner, when the separating cylinder rotates under the driving of power, the separating cylinder and the stirring blades discharge the non-glue substances which are subjected to enzymolysis into a water tank from the separating holes of the separating cylinder in the process of taking up the corresponding enzyme water solution and the eucommia bark intermediate and then throwing down, then the waste water in the water tank is discharged, and the eucommia bark intermediate with higher glue content or the final product of white filiform eucommia bark glue is left in the separating cylinder.
Further, the time for repeatedly stirring and washing the corresponding eucommia bark intermediates in the water solution of the corresponding enzyme in the step 2), the step 3) and the step 4) is 8 hours, and the time for repeatedly stirring and washing the corresponding eucommia bark intermediates in the water solution of the corresponding enzyme is 2 hours.
Further, the temperature of the hot water in the step 2), the step 3) and the step 4) is 43 degrees.
Further, in the step 2), the ingredients are prepared according to the following parts by weight, namely 100 parts of eucommia bark intermediate I, 150 parts of hot water and 12.5 parts of protease; in the step 3), various components are prepared according to the following parts by weight, namely 100 parts of eucommia bark intermediate II, 150 parts of hot water and 12.5 parts of pectinase; the components in the step 4 are prepared according to the following parts by weight: 100 parts of eucommia bark intermediate III, 150 parts of hot water and 12.5 parts of cellulase.
Has the advantages that:
1. according to the invention, after the non-glue substances of the bark of the eucommia ulmoides raw material are treated by adopting an explosion method, the protein components in the bark of the eucommia ulmoides are decomposed by adopting protease in an enzymolysis device, the intercellular wall and the intercellular layer in the bark of the eucommia ulmoides are decomposed by adopting pectinase, and the cellulose is adopted to promote the dissolution of the plant cell wall while the decomposition of cellulose and hemicellulose is improved, so that the non-glue substances in the eucommia ulmoides are decomposed into the components which can be completely separated from the gutta percha.
2. The method adopts a method that a separating cylinder in an enzymolysis device rotates in a water tank to separate a target object from waste components, the inner wall of the separating cylinder is provided with a bent plate stirring blade with an angular cross section, the included angle of the bent plate is not less than 120 degrees, the stirring blade and a support shaft form an inclination angle of 10-15 degrees, and long holes are arranged on the bent plate in a staggered mode, so that not only is the enzyme solution and corresponding eucommia bark intermediates fully stirred, but also the stirring resistance can be reduced, the energy is saved, the stirring efficiency is improved, the separation period is shortened, the thoroughness of separating the target object from the waste components is further ensured, the method completely adopts biological enzyme to decompose non-glue substances in the eucommia bark rubber, and the method is clean, environment-friendly, low in cost and high in efficiency, and the purity of extracted eucommia bark rubber can reach more than 92%.
3. The biological enzymes with different functions are used in sequence, so that the tissue structure and cells of plants can be thoroughly destroyed, the intracellular and extracellular natural components are completely diffused into the aqueous solution, the chemical structure of the target gutta percha cannot be destroyed, the biological enzymes with different functions cannot be influenced mutually, and the enzymolysis effect of each enzyme can be improved.
4. The enzymatic hydrolysis reaction of the method utilizes the catalytic hydrolysis function of enzyme, the volume of reaction liquid is small, the subsequent treatment is simple, and the discharge amount is small; the method does not contain chemical agents, and the gutta-percha is extracted by the method, so that the discharge liquid can be further processed and extracted to obtain natural medicines in the eucommia, and the requirements for obtaining high-quality gutta-percha and high-activity gutta-percha medicines are met. The final discharge can not only not cause pollution, but also can be applied to eucommia ulmoides forest as fertilizer to fertilize soil.
Drawings
FIG. 1 is a schematic structural view of an enzymatic hydrolysis apparatus.
Fig. 2 is a view a-a of fig. 1.
FIG. 3 is a schematic view of the stirring blade on the inner wall of the separation cylinder.
Fig. 4 is a view from direction B of fig. 3.
In the figure, 1, a water tank cover, 2, a feed inlet, 3, a separating cylinder, 4, a water tank, 5, a water tank interlayer, 6, water tank contents, 7, a waste liquid recovery pipe, 8, a waste residue discharge pipe, 9, a transmission driven wheel, 10, a central support shaft, 11, a support rod, 12, a separating hole, 13, a stirring blade, 1301 and an elongated hole.
Detailed Description
Example 1:
the present invention will be described in further detail with reference to the drawings and the detailed description.
A method for extracting gutta-percha by a whole biological enzyme method comprises the following steps:
1) firstly, cutting eucommia bark serving as a raw material into pieces with the area not more than 25cm by using a hay cutter2Soaking the small blocks in clean water at normal temperature for 12h, taking out, and blasting in a blasting machine to obtain cortex Eucommiae intermediate I;
in the step, the blasting temperature is controlled below 50 ℃, the blasting pressure is controlled at 1.4MPa, and the blasting time is controlled at 0.3S;
2) putting the eucommia bark intermediate I obtained in the step 1) into a protease aqueous solution, repeatedly stirring and washing for 6 hours, and then repeatedly stirring and washing for 1.5 hours by using clear water to obtain a eucommia bark intermediate II:
when washing in the step, various components are prepared according to the following parts by weight: 90 parts of eucommia bark intermediate I, 140 parts of hot water and 10 parts of protease, wherein the temperature of the hot water is controlled to be 40 ℃;
3) and putting the eucommia bark intermediate II obtained in the step 2 into a water solution of pectinase, repeatedly stirring and washing for 6 hours, and then repeatedly stirring and washing with clear water for 1.5 hours to obtain a eucommia bark intermediate III:
when washing in the step, various components are prepared according to the following parts by weight: 90 parts of eucommia bark intermediate II, 140 parts of hot water and 10 parts of pectinase, wherein the temperature of the hot water is controlled to be 40 ℃;
4) putting the eucommia bark intermediate III obtained in the step 3 into a water solution of cellulase, repeatedly stirring and washing for 6 hours, and then repeatedly stirring and washing with clear water for 1.5 hours to obtain white filamentous eucommia ulmoides gum;
when washing in the step, various components are prepared according to the following parts by weight: 90 parts of eucommia bark intermediate III, 140 parts of hot water and 10 parts of cellulase, wherein the temperature of the hot water is controlled to be 40 ℃;
the steps 2), 3) and 4) are all carried out in the same enzymolysis device, the enzymolysis device is provided with a water tank 4 and a separating cylinder 3, the separating cylinder 3 is cylindrical, the separating cylinder is horizontally arranged on the upper edge of the water tank 4 through a central supporting shaft 10, the water tank 4 is filled with the water solution of the corresponding enzyme, one part of the separating cylinder 3 is immersed in the water solution of the corresponding enzyme, the inside of the separating cylinder 3 is provided with corresponding eucommia bark intermediates, the side wall of the separating cylinder 3 is provided with a separating hole 12 with the diameter of 0.8-1.2mm, the inner wall of the separating cylinder 3 is also longitudinally provided with a stirring blade 13, the stirring blade 13 is a bent plate with the cross section being angular, the included angle of the bent plate is not less than 120 degrees, the length direction of the bent plate and the central supporting shaft 10 have an inclined angle, the inclined angle is 10-15 degrees, and the opening of the included angle of the bent plate is opposite to the rotating direction of the separating cylinder 3, and the bending plate is provided with long holes 1301 in a staggered manner, when the separating cylinder 3 rotates under the driving of power, the separating cylinder 3 and the stirring blades 13 discharge the non-glue substances which are subjected to enzymolysis into the water tank 4 from the separating holes 12 of the separating cylinder 3 in the process of taking up the corresponding enzyme water solution and the eucommia bark intermediate and then throwing down, then the waste water in the water tank 4 is discharged, and the eucommia bark intermediate with higher glue content or the final product of white thread-shaped eucommia bark glue is left in the separating cylinder.
Example 2:
a method for extracting gutta-percha by a whole biological enzyme method comprises the following steps:
1) firstly, cutting eucommia bark serving as a raw material into pieces with the area not more than 25cm by using a hay cutter2Soaking the small blocks in clean water at normal temperature for 12.5h, taking out, and blasting in a blasting machine to obtain cortex Eucommiae intermediate I;
in the step, the blasting temperature is controlled below 50 ℃, the blasting pressure is controlled at 1.5MPa, and the blasting time is controlled at 0.4S;
2) putting the eucommia bark intermediate I obtained in the step 1) into a protease aqueous solution, repeatedly stirring and washing for 6.5 hours, and then repeatedly stirring and washing for 2 hours with clear water to obtain a eucommia bark intermediate II:
when washing in the step, various components are prepared according to the following parts by weight: 100 parts of eucommia bark intermediate I, 150 parts of hot water and 12.5 parts of protease, wherein the temperature of the hot water is controlled to be 43 ℃;
3) and putting the eucommia bark intermediate II obtained in the step 2 into aqueous solution of pectinase, repeatedly stirring and washing for 6.5 hours, and then repeatedly stirring and washing with clear water for 2 hours to obtain an eucommia bark intermediate III:
when washing in the step, various components are prepared according to the following parts by weight: 100 parts of eucommia bark intermediate II, 150 parts of hot water and 12.5 parts of pectinase, wherein the temperature of the hot water is controlled to be 43 ℃;
4) putting the eucommia bark intermediate III obtained in the step 3 into a water solution of cellulase, repeatedly stirring and washing for 6.5 hours, and then repeatedly stirring and washing for 2 hours by using clear water to obtain white filamentous eucommia ulmoides gum;
when washing in the step, various components are prepared according to the following parts by weight: 100 parts of eucommia bark intermediate III, 150 parts of hot water and 12.5 parts of cellulase, wherein the temperature of the hot water is controlled to be 43 ℃;
the steps 2), 3) and 4) are all carried out in the same enzymolysis device, the enzymolysis device is provided with a water tank 4 and a separating cylinder 3, the separating cylinder 3 is cylindrical, the separating cylinder is horizontally arranged on the upper edge of the water tank 4 through a central supporting shaft 10, the water tank 4 is filled with the water solution of the corresponding enzyme, one part of the separating cylinder 3 is immersed in the water solution of the corresponding enzyme, the inside of the separating cylinder 3 is provided with corresponding eucommia bark intermediates, the side wall of the separating cylinder 3 is provided with a separating hole 12 with the diameter of 0.8-1.2mm, the inner wall of the separating cylinder 3 is also longitudinally provided with a stirring blade 13, the stirring blade 13 is a bent plate with the cross section being angular, the included angle of the bent plate is not less than 120 degrees, the length direction of the bent plate and the central supporting shaft 10 have an inclined angle, the inclined angle is 10-15 degrees, and the opening of the included angle of the bent plate is opposite to the rotating direction of the separating cylinder 3, and the bending plate is provided with long holes in a staggered manner, when the separating cylinder 3 rotates under the driving of power, the separating cylinder 3 and the stirring blades 13 discharge the non-glue substances which are subjected to enzymolysis into the water tank 4 from the separating holes 12 of the separating cylinder 3 in the process of taking up the corresponding enzyme water solution and the eucommia bark intermediate and then throwing down, then the waste water in the water tank 4 is discharged, and the eucommia bark intermediate with higher glue content or the white thread-shaped eucommia bark glue of the final product is left in the separating cylinder.
Example 3:
a method for extracting gutta-percha by a whole biological enzyme method comprises the following steps:
1) firstly, cutting eucommia bark serving as a raw material into pieces with the area not more than 25cm by using a hay cutter2Then cutting it into small piecesSoaking in clean water at normal temperature for 13h, taking out, and blasting in a blasting machine to obtain cortex Eucommiae intermediate I;
in the step, the blasting temperature is controlled below 50 ℃, the blasting pressure is controlled at 1.8MPa, and the blasting time is controlled at 0.5S;
2) putting the eucommia bark intermediate I obtained in the step 1) into a protease aqueous solution, repeatedly stirring and washing for 7 hours, and then repeatedly stirring and washing for 2.5 hours with clear water to obtain a eucommia bark intermediate II:
when washing in the step, various components are prepared according to the following parts by weight: 110 parts of eucommia bark intermediate I, 160 parts of hot water and 15 parts of protease, wherein the temperature of the hot water is controlled to be 50 ℃;
3) and putting the eucommia bark intermediate II obtained in the step 2 into a water solution of pectinase, repeatedly stirring and washing for 7 hours, and then repeatedly stirring and washing with clear water for 2.5 hours to obtain a eucommia bark intermediate III:
when washing in the step, various components are prepared according to the following parts by weight: 110 parts of eucommia bark intermediate II, 160 parts of hot water and 15 parts of pectinase, wherein the temperature of the hot water is controlled to be 50 ℃;
4) putting the eucommia bark intermediate III obtained in the step 3 into a water solution of cellulase, repeatedly stirring and washing for 6.5 hours, and then repeatedly stirring and washing for 2 hours by using clear water to obtain white filamentous eucommia ulmoides gum;
when washing in the step, various components are prepared according to the following parts by weight: 110 parts of eucommia bark intermediate III, 160 parts of hot water and 15 parts of cellulase, wherein the temperature of the hot water is controlled to be 50 ℃;
the steps 2), 3) and 4) are all carried out in the same enzymolysis device, the enzymolysis device is provided with a water tank 4 and a separating cylinder 3, the separating cylinder 3 is cylindrical, the separating cylinder is horizontally arranged on the upper edge of the water tank 4 through a central supporting shaft 10, the water tank 4 is filled with the water solution of the corresponding enzyme, one part of the separating cylinder 3 is immersed in the water solution of the corresponding enzyme, the inside of the separating cylinder 3 is provided with corresponding eucommia bark intermediates, the side wall of the separating cylinder 3 is provided with a separating hole 12 with the diameter of 0.8-1.2mm, the inner wall of the separating cylinder 3 is also longitudinally provided with a stirring blade 13, the stirring blade 13 is a bent plate with the cross section being angular, the included angle of the bent plate is not less than 120 degrees, the length direction of the bent plate and the central supporting shaft 10 have an inclined angle, the inclined angle is 10-15 degrees, and the opening of the included angle of the bent plate is opposite to the rotating direction of the separating cylinder 3, and the bending plate is provided with long holes in a staggered manner, when the separating cylinder 3 rotates under the driving of power, the separating cylinder 3 and the stirring blades 13 discharge the non-glue substances which are subjected to enzymolysis into the water tank 4 from the separating holes 12 of the separating cylinder 3 in the process of taking up the corresponding enzyme water solution and the eucommia bark intermediate and then throwing down, then the waste water in the water tank 4 is discharged, and the eucommia bark intermediate with higher glue content or the white thread-shaped eucommia bark glue of the final product is left in the separating cylinder.
The enzymatic hydrolysis device used in the invention is also provided with a water tank cover 1 which can prevent the water tank contents 6 from splashing outwards to pollute the environment, a feed inlet 2 for additionally installing eucommia bark raw materials is arranged on the separation cylinder 3, a heater is arranged in the water tank interlayer 5, heat can be transferred to the solution in the water tank 4 by heating the water in the water tank interlayer 5, the bottom of the water tank 4 is provided with a waste liquid recovery pipe 7 and a waste residue discharge pipe 8 for discharging waste, the waste liquid recovery pipe 7 is communicated with a waste liquid recovery system and can recover other useful components in the waste liquid, the waste residue discharge pipe 8 can discharge waste solid residue which is used as fertilizer for plants, and a transmission driven wheel 9 is arranged on a central support shaft 10 of the separation cylinder 3 and can drive the separation cylinder 3 to rotate under the drive of a power device.
Because the method adopts the enzymatic hydrolysis device to extract by adopting the biological enzyme method, the equipment cost is low, the production process is safe and environment-friendly, the extracted gutta-percha can keep the natural physical performance, no damage is caused to non-glue components, the utilization rate of the whole raw material of the eucommia bark is improved, and the method is suitable for industrialized popularization and utilization.
Claims (4)
1. A method for extracting gutta-percha by a full-biological enzyme method is characterized by comprising the following steps:
1) firstly, cutting eucommia bark serving as a raw material into pieces with the area not larger than the area by using a hay cutter25cm2Soaking the small blocks in clean water at normal temperature for more than 12h, taking out, and blasting in a blasting machine to obtain cortex Eucommiae intermediate I;
in the step, the blasting temperature is controlled below 50 ℃, the blasting pressure is controlled between 1.4MPa and 1.8MPa, and the blasting time is controlled within 0.5S;
2) putting the eucommia bark intermediate I obtained in the step 1) into a protease aqueous solution, repeatedly stirring and washing for 6-7h, then repeatedly stirring and washing for 1.5-2.5h with clear water to obtain an eucommia bark intermediate II:
when washing in the step, various components are prepared according to the following parts by weight: 90-110 parts of eucommia bark intermediate I, 160 parts of hot water 140 and 10-15 parts of protease, wherein the temperature of the hot water is controlled to be 40-50 ℃;
3) putting the eucommia bark intermediate II obtained in the step 2 into aqueous solution of pectinase, repeatedly stirring and washing for 6-7h, then repeatedly stirring and washing for 1.5-2.5h with clear water to obtain an eucommia bark intermediate III:
when washing in the step, various components are prepared according to the following parts by weight: 90-110 parts of eucommia bark intermediate II, 160 parts of hot water 140 and 10-15 parts of pectinase, wherein the temperature of the hot water is controlled to be 40-50 ℃;
4) putting the eucommia bark intermediate III obtained in the step 3 into a water solution of cellulase, repeatedly stirring and washing for 6-7h, and then repeatedly stirring and washing with clear water for 1.5-2.5h to obtain white filamentous eucommia ulmoides gum;
when washing in the step, various components are prepared according to the following parts by weight: 90-110 parts of eucommia bark intermediate III, 160 parts of hot water 140 and 10-15 parts of cellulase, wherein the temperature of the hot water is controlled to be 40-50 ℃;
the steps 2), 3) and 4) are all carried out in the same enzymolysis device, the enzymolysis device is provided with a water tank and a separating cylinder, the separating cylinder is cylindrical and is horizontally arranged on the upper edge of the water tank through a central supporting shaft, the water tank is filled with the water solution of the corresponding enzyme, one part of the separating cylinder is immersed in the water solution of the corresponding enzyme, the inside of the separating cylinder is provided with the corresponding eucommia bark intermediate, the side wall of the separating cylinder is provided with a separating hole with the diameter of 0.8-1.2mm, the inner wall of the separating cylinder is also longitudinally provided with a stirring blade, the stirring blade is a bent plate with the cross section being angular, the included angle of the bent plate is not less than 120 degrees, the length direction of the bent plate and the central supporting shaft have the inclined angle, the inclined angle is 10-15 degrees, and the opening of the included angle of the bent plate is opposite to the rotating direction of the separating cylinder, and the bending plate is provided with elongated holes in a staggered manner, when the separating cylinder rotates under the driving of power, the separating cylinder and the stirring blades discharge the non-glue substances which are subjected to enzymolysis into a water tank from the separating holes of the separating cylinder in the process of taking up the corresponding enzyme water solution and the eucommia bark intermediate and then throwing down, then the waste water in the water tank is discharged, and the eucommia bark intermediate with higher glue content or the final product of white filiform eucommia bark glue is left in the separating cylinder.
2. The method for extracting gutta-percha by the holobiological enzyme method according to claim 1, which is characterized in that: in the step 2), the step 3) and the step 4), the time for repeatedly stirring and washing the corresponding eucommia bark intermediates in the corresponding enzyme water solution is 8 hours, and the time for repeatedly stirring and washing the corresponding eucommia bark intermediates by using clean water is 2 hours.
3. The method for extracting gutta-percha by the holobiological enzyme method according to claim 1, which is characterized in that: the temperature of the hot water in the step 2), the step 3) and the step 4) is 43 ℃.
4. The method for extracting gutta-percha by the holobiological enzyme method according to claim 1, which is characterized in that: in the step 2), the ingredients are prepared according to the following parts by weight, namely 100 parts of eucommia bark intermediate I, 150 parts of hot water and 12.5 parts of protease; in the step 3), various components are prepared according to the following parts by weight, namely 100 parts of eucommia bark intermediate II, 150 parts of hot water and 12.5 parts of pectinase; the components in the step 4 are prepared according to the following parts by weight: 100 parts of eucommia bark intermediate III, 150 parts of hot water and 12.5 parts of cellulase.
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| CN105661519A (en) * | 2016-01-28 | 2016-06-15 | 张萌萌 | Method for preparing healthcare eucommia ulmoides medicine powder through enzymolysis of eucommia ulmoides leaves |
| CN105832807A (en) * | 2016-03-24 | 2016-08-10 | 浙江旭源杜仲生物科技有限公司 | Degradation method for eucommia ulmoides bark and arbor bark plant tissue structure |
| CN108707254A (en) * | 2018-06-08 | 2018-10-26 | 贵州艾科米亚生物科技有限公司 | A kind of new rubber material Cortex Eucommiae-Hevea rubber of biology base |
| CN114214257B (en) * | 2022-01-12 | 2023-10-31 | 河南省商业科学研究所有限责任公司 | Soil fungus screening and separating method |
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| CN101157827A (en) * | 2007-09-27 | 2008-04-09 | 贵州大学 | Method for extracting filament gutta-percha from eucommia leaf and skin |
| CN102838757A (en) * | 2012-08-28 | 2012-12-26 | 河南恒瑞源实业有限公司 | Method of extracting gutta-percha through enzymolysis |
| CN102863628A (en) * | 2012-11-05 | 2013-01-09 | 河南恒瑞源实业有限公司 | Method for preparing eucommia rubber by using eucommia seeds |
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