CN104328530A - Biodegradable antibacterial composite fibers and preparation method thereof - Google Patents
Biodegradable antibacterial composite fibers and preparation method thereof Download PDFInfo
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- CN104328530A CN104328530A CN201410524073.0A CN201410524073A CN104328530A CN 104328530 A CN104328530 A CN 104328530A CN 201410524073 A CN201410524073 A CN 201410524073A CN 104328530 A CN104328530 A CN 104328530A
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Abstract
The present invention relates to biodegradable antibacterial composite fibers, which comprise polylactic acid, nanoparticles and a surfactant, wherein the nanoparticles are nanometer zinc oxide or nanometer titanium oxide, and a weight ratio of the polylactic acid to the nanoparticles to the surfactant is 100:2-10:0.08-1.2. The present invention further provides a preparation method of the biodegradable antibacterial composite fibers, wherein the preparation method comprises a polylactic acid solution preparation step. The preparation method specifically comprises: dissolving polylactic acid in a mixed solvent, wherein the polylactic acid concentration is 10-15 g/L; adding nanoparticles and a surfactant to the polylactic acid solution, and completely mixing to form a mixed solution; carrying out an ultrasonic dispersion treatment on the mixed solution; and placing the stable suspension solution after being subjected to the ultrasonic dispersion treatment in a preset syringe, and carrying out a spinning treatment on the suspension solution according to the preset injection speed. According to the present invention, the preparation method is simple and feasible, and the prepared composite fibers have a certain strength, good biological safety, and good antibacterial property.
Description
Technical field
The invention belongs to materialogy field, particularly relate to a kind of Biocomposite material, specifically a kind of degradable antibacterial composite fibers and preparation method thereof.
Background technology
Along with fast pace life and social senilization's trend of modern society, people propose more and more higher requirement to field of medical technology.Accelerating wound healing is one of emphasis of current skin treating.Research shows, the moistening degree of surface of a wound environment and antimicrobial levels are the principal elements affecting wound healing.Common medical dressing is made up of cotton fibre, do not have restraining and sterilizing bacteria effect, only can shield a small amount of bacterium, therefore the easy breed bacteria of dressing surface.And a kind of specific medical dressing with high moisturizing and high antibacterial functions plays an important role over the course for the treatment of, become the study hotspot of field of medical materials, this " artificial skin " of namely often saying.Artificial skin is not only for the protection of the surface of a wound and antibacterial, sterilization; can also Cell differentiation inducing activity, promote skin cell proliferation; by Matrix absorption, and can not only can grow together with human body neoplastic skin in wound, and substituted human body skin completely before human normal skin is formed.Artificial skin is carrier with three-dimensional rack, has certain mechanical strength, is conducive to sticking and playing its normal function, as cell proliferation, distortion, migration and differentiation etc. of cell.Therefore, in the structure of artificial skin, timbering material serves crucial effect.
The kind of artificial skin timbering material is a lot, can be divided into synthesis class and biological species by its chemical property.Wherein synthesize class timbering material mainly with polyesters such as polyactide, polylactone, PHA, Merlon, as PLA (PLA), polyglycolic acid (PGA), lactic acid-ethanol copolymer (PLGA) etc.; Biological species timbering material is mainly based on collagen gel and collagen sponge.The method preparing polymer support is a lot, as method of electrostatic spinning, self-assembly method and phase separation method etc.
Antiseptic is the antibacterial active ingredient of artificial skin.Antiseptic can be divided into organic antibacterial agent and the large class of inorganic antiseptic two usually.Poor, the easy decomposition of organic antibacterial agent heat endurance, persistence are poor, and usual toxicity is comparatively large, long-time use harmful.In order to overcome the shortcoming of organic antibacterial agent, research direction has been turned to inorganic antiseptic by people gradually, as zinc oxide (ZnO), titanium oxide (TiO
2) and silver (Ag) etc.
But in the prior art, a lot of to the preparation method of antibacterial thin polymer film, but all cannot realize, to dispersed in polymer spun fibers of nano antibacterial agent, cause patient skin treatment thus and there is larger uncertainty, infection probability is high, and the treatment of patient is experienced not good.
Summary of the invention
For above-mentioned technical problem of the prior art, the invention provides a kind of degradable antibacterial composite fibers and preparation method thereof, described this degradable antibacterial composite fibers and preparation method thereof will solve biomaterial of the prior art for there is larger uncertainty during skin treating, infecting the technical problem that probability is high, the treatment of patient experience is not good.
The invention provides a kind of degradable antibacterial composite fibers, be made up of PLA, nano particle and surfactant, in described composite fibre, the weight ratio of described PLA, nano particle and surfactant is 100:2 ~ 10:0.08 ~ 1.2, and described nano particle is nano zine oxide or nano-titanium oxide.
Present invention also offers the preparation method of above-mentioned degradable antibacterial composite fibers, comprise the steps:
1) step of PLA solution is prepared for one, prepare in the step of PLA solution described, first prepare an organic mixed solvent, then take PLA, PLA is dissolved in mixed solvent, the concentration 10 ~ 15g/L of described PLA in mixed solvent;
2) nano particle is taken according to the weight ratio of nano particle and PLA, take surfactant simultaneously, the quality of surfactant is 4 ~ 12% of nanoparticle mass, nano particle and surfactant is joined in PLA solution and is thoroughly mixed to form mixed solution;
3) the above-mentioned mixed solution prepared is carried out ultrasonic wave dispersion treatment;
4) aaerosol solution stable after ultrasonic wave dispersion treatment is positioned in default syringe, and according to the injection speed preset, spinning process is carried out to described aaerosol solution.
Further, described organic mixed solvent is made up of carrene, DMF, and described carrene, the mass ratio of DMF are 1:1.
Further, described surfactant is tween Tween60 or tween Tween80.
Further, when nano particle is nano-ZnO, described surfactant adopts tween Tween60, and the quality of tween Tween60 is 12% of nano-ZnO.
Further, when nano particle is nano-ZnO, described surfactant adopts tween Tween60, and the quality of tween Tween60 is 12% of nano-ZnO quality.
Further, when nano particle is nano-TiO
2, described surfactant adopts tween Tween80, and the quality of tween Tween80 is nano-TiO
210% of quality.
Further, when nano particle is nano-ZnO, phaco time is 10 ~ 20min; When nano particle is nano-TiO
2, phaco time is 10 ~ 20min.
Further, described aaerosol solution is carried out in the process of spinning process, by described stable aaerosol solution as in the disposable syringe of electrostatic spinning, and carry out spinning with the injection speed of 0.5 ~ 2ml/h, spinning voltage is 10 ~ 20kv, and the distance of spinning nozzle and receiver is 15 ~ 25cm.
Further, when carrying out spinning process to described aaerosol solution, room temperature keeps 20 ~ 30 DEG C, and humidity keeps 40 ~ 50%.
Nano zine oxide, nano-titanium oxide are except having the antibacterial action of conventional oxidation zinc, because particle diameter reaches nanoscale, there is the distinctive surface-interface effect of nano particle, being embodied in antibacterial effect aspect is: the surface atom quantity of nano particle is greatly more than conventional particle, surface atom possesses very high energy owing to lacking contiguous coordination atom, the affinity of zinc oxide and bacterium can be strengthened, improve antibacterial efficiency.
Method of electrostatic spinning uses high-tension electricity field force to stretch to having certain density polymer drop, and drop becomes thread subsequently, and along with the volatilization of solvent, liquid stream solidifies gradually finally to fall within aluminium foil receiver and forms a skim.This film has that porosity is high, specific area is large, fiber fine degree and the advantage such as homogeneity is high, draw ratio is large, thus imparts electricity spinning fibre application prospect widely.
The present invention obtains degradable antibacterial composite fibers by electrostatic spinning technique, by adopting original position spin processes, to finely dispersed inorganic nano antiseptic and polymer solution in a polymer solution, by method of electrostatic spinning, make the film with loose structure.Method simple possible of the present invention, made composite fibre has certain intensity, and biological safety is better, and antibiotic property is better.Can be effective to treat skin injury, prevent skin ulceration from infecting, reduce the infection probability of skin patient, the treatment improving patient is experienced.
Accompanying drawing explanation
Fig. 1 is the flow chart of the preparation method of a kind of degradable antibacterial composite fibers of the present invention.
Fig. 2 is ZnO/PLA mixed solution electrostatic spinning schematic diagram.
Fig. 3 is the deployment conditions schematic diagram of nano-ZnO in PLA solution.
Fig. 4 is nano-TiO
2deployment conditions schematic diagram in PLA solution.
Fig. 5 is the SEM microscopic appearance schematic diagram of ZnO/PLA composite cellulosic membrane.
Fig. 6 is that different ZnO content affects schematic diagram to composite cellulosic membrane is hydrophilic.
Fig. 7 is different Ti O
2content affects schematic diagram to composite cellulosic membrane is hydrophilic.
Fig. 8 is that in spinning film, different ZnO content affects schematic diagram to cytoactive.
Fig. 9 is the cell adherence situation schematic diagram of composite cellulosic membrane.
Figure 10 is PLA/ TiO
2the cytotoxicity schematic diagram of degradable polymer composite cellulosic membrane.
Detailed description of the invention
Below in conjunction with accompanying drawing, the embodiment of the present invention is described further.
Embodiment 1
As shown in Figure 1, a kind of preparation method of degradable antibacterial composite fibers, comprises the steps:
1) PLA solution is configured;
The PLA solid particle measuring 12mg with electronic balance is respectively poured in brown, wide-mouth bottle, adds 10ml mixed solvent, is placed on magnetic stirrer until solid dissolves completely at 25 DEG C.Polylactic acid molecule amount is 200,000Da, and mixed solvent is carrene: DMF mixes by 1:1.
2) nano particle is fully mixed generation miscible fluid with PLA solution, and under the effect of surfactant, ultrasonic wave dispersion treatment is carried out to described mixed solution;
Parameter is as follows: nano-particle content is respectively: 2%, 4%, 6%, 8% and 10% of PLA quality.Surfactant: tween Tween60(is adapted to nano-ZnO system)--12% of ZnO quality; Tween Tween80(is adapted to nano-TiO
2system)--TiO
210% of quality.Phaco time: ZnO/PLA system 15min; TiO
2/ PLA system 15min.
3) after ultrasonic wave dispersion treatment is carried out to described mixed solution, aaerosol solution stable after described process is positioned in default syringe, and according to the injection speed preset, spinning process is carried out to described aaerosol solution.
After treating that two individual system are ultrasonic, by stable aaerosol solution as in the disposable syringe of electrostatic spinning, and carry out spinning with the injection speed of 1ml/h, spinning voltage is 15kv, and the distance of spinning nozzle and receiver is 20cm.Room temperature keeps 25 DEG C, and humidity keeps 45%.
The present invention is successfully obtained containing nano-ZnO (TiO by original position method of electrostatic spinning
2) degradable antibacterial composite fibers.And by ESEM, morphology observation is carried out to composite fibre, utilize contact angle instrument to study composite fiber surface hydrophilicity.Finally, antibacterial tests will be carried out to composite fibre, study different ZnO(TiO
2) addition is to the effect of its anti-microbial property.
Nano particle is very easily reunited in a liquid, thus affects the performance of its performance.Therefore, in order to improve nano-ZnO, TiO
2suspension stability in polylactic acid PLA solution, the embodiment of the present invention can select two kinds of different surfactants (tween Tween60 and tween Tween80), by changing different surface-active contents (4%, 6%, 8%, 10% and 12%) and different ultrasonic emulsification jitter times (15 minutes, 30 minutes, 60 minutes and 90 minutes) determine best composition and engineering, to ensure nano-TiO
2good suspension stability can be remained in the operating process of electrostatic spinning.The dispersing matrixes that the embodiment of the present invention adopts is the PLA solution of 12%, and the compound system of carrene and DMF selected by solvent, and the two ratio is 1:1.With nano-ZnO, TiO
2in PLA solution, the length of suspension time is as reference foundation and evaluation criterion.
For nano-ZnO, embodiment of the present invention electrospinning device used can as shown in Figure 2, be made up of DC generator 1, two pass micro-injection pump 2 and superfine fibre board joint receiving apparatus 3.Concrete operations can comprise employing disposable sterilized injector and extract a certain amount of solution 6 to be spun, and are connected with stainless steel spinning nozzle 4 by syringe needle with conduit, and spinning nozzle 4 other end is then connected with wire with DC generator 1.Now set spinning voltage, adjustment jet path length, controls disposable syringe injection speed, solution 6 to be spun is flowed out from shower nozzle with certain speed.Due to by the effect of steady electric field, the drop formed at shower nozzle place becomes taylor cone gradually, and then is drawn into thread, and thread is solidified along with solvent evaporates, after a period of time, receiving system 3 can receive electrospinning fibre film 5.
The suspension stability of embodiment 2 pairs of nano-ZnOs is tested
The suspension stability result of study of the ZnO/PLA solution being surfactant with polysorbate60 and Tween 80 is as shown in table 3, table 3 can be used for showing that the content of surfactant and kind affect ZnO sedimentation situation, wherein, (×) represents within observing time without sedimentation or lamination;
(√) there is sedimentation or lamination in expression within observing time; .
Table 1
Can choose in above table, the not stratified formula of 3 groups of good mixings carries out follow-up TEM test, and result of the test is as shown in table 2, and table 2 is tem observation formula.
Table 2
| Kind | Content is determined | Ultrasonic time |
| Tween60 | 12% | 15min |
| Tween60 | 12% | 30min |
| Tween80 | 8% | 60min |
Embodiment 3 pairs of nano-TiOs
2suspension stability is tested
For table 3, the embodiment of the present invention adopts the PLA solution of 12% as dispersing matrixes, selects the compound system of carrene and DMF (1:1) to make solvent, by a certain amount of nano-TiO
2be placed in solution carry out certain hour ultrasonic wave dispersion after, observe the sedimentation time of nano particle in PLA solution.This experiment is with nano-TiO
2in PLA solution, stable suspersion conduct in 4 hours is with reference to foundation and evaluation criterion.Experimental result is as shown in table 3, and table 3 is for representing that different surfaces active species and content are to nano-TiO
2the impact of suspension stability, nano-TiO in this form
2suspension stability observing time is 240min.
Table 3
Embodiment 4 nano antibacterial agent deployment conditions transmission electron microscope TEM schemes
Being separated into example with nano-ZnO, sample longer for ZnO suspension time in settling test is prepared again, for observing its deployment conditions under transmission electron microscope, finding that the sample that not all suspension stability is good must be just the uniform system of nanoparticulate dispersed.This may be less relevant with the particle of nano-ZnO own.The nano-ZnO selected be particle diameter at about 50nm, therefore, a small amount of reunion might not cause its sedimentation, therefore there will be the good system of a lot of suspension stability.And real from transmission electron microscope observing, just find to select Tween60 when surfactant, and content is 12% time, its ultrasonic time is when 15min, and dispersion effect is best, and effect can be as shown in Figure 3.Correspondingly, nano-TiO
2deployment conditions can be as shown in Figure 4.Comprehensive suspension stability test and TEM photo, when known selection Tween 80 makees surfactant, it is to TiO
2the stability of dispersion is better than polysorbate60, therefore chooses the surfactant that Tween 80 is follow-up work.Therefore embodiment of the present invention spinning have chosen Tween 80 concentration is 10% water bath sonicator, 15 minutes these formulas.
Embodiment 5 observes the microscopic appearance (SEM) of spinning fibre film
Combining nano ZnO, TiO
2the result of suspension stability and the photo of transmission electron microscope, finally choose electrostatic spinning formula and carry out follow-up spinning operation.As shown in Figure 5, Fig. 5 is the SEM microscopic appearance figure of ZnO/PLA composite cellulosic membrane to the microscopic appearance of obtained composite cellulosic membrane.As can be seen from Figure 5, containing nano-ZnO or TiO
2after the PLA solution-polymerized SBR of particle, the spinning film of formation is non-woven constructions, and film thickness is comparatively even, and occurs without beading or drop, this illustrate selected by experimental condition be suitable.In figure, spinning diameter is about about 300nm, and smooth surface, situation inter-adhesive between silk and silk is less.
Embodiment 6 can be studied spinning fibre film hydrophily
PLA is a kind of oil loving polymer, therefore have impact on its use in human body.As shown in Figure 6, Fig. 6 is that different ZnO content affects schematic diagram to composite cellulosic membrane is hydrophilic, is introduced by ZnO in PLA, has carried out hydrophily test by contact angle instrument to composite cellulosic membrane.Research finds, along with the increase of ZnO content, the hydrophily of PLA film also significantly increases.Specifically, when ZnO content is increased to 8% by 0%, the contact angle of spinning film and water is reduced to 91.09 ° by initial 124.86 °.As shown in Figure 7, Fig. 7 is different Ti O
2content affects schematic diagram, by TiO to composite cellulosic membrane is hydrophilic
2introduce in PLA, carried out hydrophily test by contact angle instrument to composite cellulosic membrane, research finds, along with TiO
2the increase of content, the hydrophily of PLA film also significantly increases, and works as TiO
2when content is increased to 8% by 0%, the contact angle of spinning film and water has been reduced to 99.64 ° by initial 124.86 °.
Embodiment 7 can the Study of cytotoxicity of spinning fibre film
The cytotoxicity of nano-ZnO/PLA composite fibre is as follows, the embodiment of the present invention pay close attention to emphatically prepared by microspun fiber and SF Dual culture certain hour (72hrs) after, investigate emphatically the apoptosis whether nano-ZnO content can cause cell, i.e. the evaluation of vitro cytotoxicity aspect.Fig. 8 is that in spinning film, different ZnO content affects schematic diagram to cytoactive.As can be seen from Figure 8, same polymer solution, different ZnO content is in the concentration range of 0.0625 ~ 1 mg/mL, mean percent cell survival is between 80% ~ 100%, cell-proliferation activity is not subject to obvious suppression, compares there was no significant difference (p>0.05) with control group.Test shows, PLA is as carrier material, and after with the addition of nano-ZnO, the activity of composite cellulosic membrane to Mouse Skin Fibroblasts does not have inhibitory action, does not have toxicity to cell.In conjunction with the result of cell toxicity test, we pass through the scanning electron microscopic observation Adhesion property of material to cell.Fig. 9 is the cell adherence situation schematic diagram of composite cellulosic membrane, wherein (A) is the sample topography before cell adhesion, (B) be the sample topography after cell adhesion, as can be seen from Figure 9, stem cell is after carrying out Dual culture with spinning film, all show good activated state, namely all can put out the feelers unfolds in spinning surface, what have even trails to spinning inner space, this explanation can not cause Apoptosis in the existence of nano-ZnO after spinning, proves that the biological safety of this composite spinning film is better.
Nano-TiO
2the cytotoxicity of/PLA composite fibre is as follows, and as shown in Figure 10, Figure 10 is PLA/ TiO
2the cytotoxicity schematic diagram of degradable polymer composite cellulosic membrane.As can be seen from the figure, same polymer solution, different Ti O
2content is in the concentration range of 0.0625 ~ 1 mg/mL, and mean percent cell survival is between 90% ~ 110%, and the activity of cell is not subject to obvious suppression, compares there was no significant difference (p>0.05) with control group.Test shows, PLA, as carrier material, with the addition of nano-TiO
2after, the activity of composite cellulosic membrane to Mouse Skin Fibroblasts does not have inhibitory action, does not have toxicity to cell.We adopt human bone marrow stem cell as model cell, with PLA/ TiO
2after degradable polymer composite cellulosic membrane Dual culture 24h, observe the situation of its adherent cell, in order to investigate the toxicity of material to cell.Can find out, after stem cell Dual culture 24h, there is Swelling in spinning fibre film, and this may with nutrient solution used or to clean base fluid relevant.But it is worth noting that cell adheres to well on spinning film, and put out the feelers and unfold in spinning surface, what have even trails to spinning inner space, this illustrates at nano-TiO after spinning
2existence can not cause Apoptosis, prove that the biological safety of this composite spinning film is better.
Embodiment 8 can judge that the antibacterial tests detection of material is carried out in the bacterial restrain experiment of nano-ZnO/PLA composite fibre
For ZnO, because nano-ZnO is a kind of antiseptic of photocatalyst-type, in the sun, particularly under UV-irradiation, in water and air, the electronegative electronics that energy disintegrate goes out to move freely, leaves the hole of positively charged to nano-ZnO simultaneously.Hole can excited oxygen and hydroxyl, makes the superoxide anion (0 that absorption water and air thereon becomes active
2 -) and hydroxyl free radical (OH), they have very strong redox, make cell membrane damage and cause the death of bacterium.Result of the test is as following table 4.
Table 4 is the bacterial restrain experimental result (mm) of PLA/ZnO composite cellulosic membrane to two kinds of bacterial classifications
| Bacterial classification | 0%ZnO | 2%ZnO | 4%ZnO | 5%ZnO | 8%ZnO | 10%ZnO |
| Escherichia coli | 0 | 22.3 | 26.1 | 28.7 | 32.0 | 26.5 |
| Staphylococcus aureus | 0 | 19.1 | 22.4 | 25.4 | 28.6 | 24.7 |
The diameter of bacterial restrain embodies the power of this kind of material anti-microbial property.As can be seen from the table, no matter be Escherichia coli or staphylococcus aureus, the anti-microbial property of composite cellulosic membrane is all along with the increase of ZnO content presents the rear weak trend of first enhancing.Especially, when ZnO content reaches 8%, the diameter of its antibacterial ring is respectively 32.0mm (Escherichia coli) and 28.6mm (staphylococcus aureus).Analyzing the reason that flex point appears in antibacterial tests, may be because nano-powder adds too much, easily more coated by PLA macromolecular chain, forms chain group and even causes reuniting, do not have the antibiotic and sterilizing effect that effectively can play ZnO.
Alternatively, to TiO
2/ PLA, nano-TiO
2the bacteriostatic experiment result of/PLA composite cellulosic membrane is as shown in table 5,
Table 5 PLA/ TiO
2composite cellulosic membrane is to the bacterial restrain experimental result (mm) of two kinds of bacterial classifications
| Bacterial classification | 0% TiO 2 | 2% TiO 2 | 4% TiO 2 | 5% TiO 2 | 8% TiO 2 | 10% TiO 2 |
| Escherichia coli | 0 | 17.5 | 19.7 | 20.1 | 25.8 | 29.3 |
| Staphylococcus aureus | 0 | 16.3 | 18.1 | 19.6 | 23.7 | 25.4 |
The diameter of bacterial restrain embodies the power of this kind of material anti-microbial property.As can be seen from the table, no matter be Escherichia coli or staphylococcus aureus, the anti-microbial property of composite cellulosic membrane is all along with TiO
2the increase of content and strengthening.Work as TiO
2when content reaches 10%, the diameter of its antibacterial ring is respectively 29.3mm (Escherichia coli) and 25.4mm (staphylococcus aureus).Analyzing reason may be work as TiO
2when content increases, after UV-irradiation, increasing electron-hole pair (e-and h+) will be formed on tunica fibrosa surface.In photocatalytic semiconductor, hole has larger reactivity, in water and air system, can with the H of adsorption
2o and OH-ionic reaction generates the hydroxyl radical free radical with strong oxidizing property.In addition, the molecular oxygen of electronics and adsorption reacts, and molecular oxygen not only participates in reduction reaction, or another source of surface hydroxyl free radical.The cell membrane of the active Superoxide anion free radical (O2-) that reaction generates and hydroxyl radical free radical (OH) energy penetration cell, destroy cellulose, enter thalline and stop the transmission of film forming matter, block its respiratory system and electric transmission system, thus kill bacteria effectively.That is TiO
2sterilization flow process be: (1) TiO
2catalytic water, makes to produce active oxygen in water; (2) active oxygen sterilizing; (3) bacterial death.
By reference to the accompanying drawings the embodiment of the present invention is exemplarily described above; obvious embodiment of the present invention specific implementation is not subject to the restrictions described above; as long as have employed the improvement of the various unsubstantialities that method is conceived and technical scheme is carried out of the embodiment of the present invention; or the design of the embodiment of the present invention and technical scheme directly applied to other occasion, all within the protection domain of the embodiment of the present invention without to improve.
Claims (10)
1. a degradable antibacterial composite fibers, it is characterized in that: be made up of PLA, nano particle and surfactant, in described composite fibre, the weight ratio of described PLA, nano particle and surfactant is 100:2 ~ 10:0.08 ~ 1.2, and described nano particle is nano zine oxide or nano-titanium oxide.
2. a kind of degradable antibacterial composite fibers according to claim 1, is characterized in that: described surfactant is tween Tween60 or tween Tween80.
3. the preparation method of a kind of degradable antibacterial composite fibers according to claim 1, is characterized in that comprising the steps:
A step preparing PLA solution, prepares in the step of PLA solution described, first prepares an organic mixed solvent, then takes PLA, be dissolved in by PLA in mixed solvent, the concentration 10 ~ 15g/L of described PLA in mixed solvent;
Weight ratio according to nano particle and PLA takes nano particle, take surfactant simultaneously, the quality of surfactant is 4 ~ 12% of nanoparticle mass, nano particle and surfactant is joined in PLA solution and is thoroughly mixed to form mixed solution;
The above-mentioned mixed solution prepared is carried out ultrasonic wave dispersion treatment;
Aaerosol solution stable after ultrasonic wave dispersion treatment is positioned in default syringe, and according to the injection speed preset, spinning process is carried out to described aaerosol solution.
4. the preparation method of a kind of degradable antibacterial composite fibers according to claim 3, it is characterized in that: described organic mixed solvent is by carrene, N, dinethylformamide forms, and described carrene, the mass ratio of DMF are 1:1.
5. the preparation method of a kind of degradable antibacterial composite fibers according to claim 3, is characterized in that: described surfactant is tween Tween60 or tween Tween80.
6. the preparation method of a kind of degradable antibacterial composite fibers according to claim 3, is characterized in that: when nano particle is nano-ZnO, and described surfactant adopts tween Tween60, and the quality of tween Tween60 is 12% of nano-ZnO quality.
7. the preparation method of a kind of degradable antibacterial composite fibers according to claim 3, is characterized in that: when nano particle is nano TiO 2, and described surfactant adopts tween Tween80, and the quality of tween Tween80 is nano-TiO
210% of quality.
8. the preparation method of a kind of degradable antibacterial composite fibers according to claim 3, it is characterized in that: when nano particle is nano-ZnO, phaco time is 10 ~ 20min; When nano particle is nano-TiO
2, phaco time is 10 ~ 20min.
9. the preparation method of a kind of degradable antibacterial composite fibers according to claim 3, it is characterized in that: described aaerosol solution is carried out in the process of spinning process, by described stable aaerosol solution as in the disposable syringe of electrostatic spinning, and carry out spinning with the injection speed of 0.5 ~ 2ml/h, spinning voltage is 10 ~ 20kv, and the distance of spinning nozzle and receiver is 15 ~ 25cm.
10. the preparation method of a kind of degradable antibacterial composite fibers according to claim 3, is characterized in that: when carrying out spinning process to described aaerosol solution, and room temperature keeps 20 ~ 30 DEG C, and humidity keeps 40 ~ 50%.
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