CN104328153A - Method for producing chiral enol by using a cell process - Google Patents
Method for producing chiral enol by using a cell process Download PDFInfo
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- CN104328153A CN104328153A CN201410537233.5A CN201410537233A CN104328153A CN 104328153 A CN104328153 A CN 104328153A CN 201410537233 A CN201410537233 A CN 201410537233A CN 104328153 A CN104328153 A CN 104328153A
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- cyclohexylidene
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- ramie gauze
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- 238000000034 method Methods 0.000 title claims abstract description 21
- 150000002085 enols Chemical class 0.000 title description 2
- 238000004519 manufacturing process Methods 0.000 title description 2
- 239000000758 substrate Substances 0.000 claims abstract description 70
- 240000008564 Boehmeria nivea Species 0.000 claims abstract description 64
- 238000006243 chemical reaction Methods 0.000 claims abstract description 42
- 241000235056 Pichia norvegensis Species 0.000 claims abstract description 20
- 210000005253 yeast cell Anatomy 0.000 claims abstract description 20
- 210000004027 cell Anatomy 0.000 claims abstract description 16
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 54
- 239000000243 solution Substances 0.000 claims description 36
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 19
- 239000008103 glucose Substances 0.000 claims description 19
- 238000009423 ventilation Methods 0.000 claims description 17
- 235000015097 nutrients Nutrition 0.000 claims description 16
- 239000000203 mixture Substances 0.000 claims description 14
- 230000002210 biocatalytic effect Effects 0.000 claims description 13
- 239000000843 powder Substances 0.000 claims description 13
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 12
- 238000000605 extraction Methods 0.000 claims description 12
- 238000002360 preparation method Methods 0.000 claims description 12
- AWAKCWDDISOXOC-QMMMGPOBSA-N (2r)-3-cyclohexylidene-2,3-dihydroxypropanal Chemical compound O=C[C@H](O)C(O)=C1CCCCC1 AWAKCWDDISOXOC-QMMMGPOBSA-N 0.000 claims description 9
- 239000007787 solid Substances 0.000 claims description 9
- 239000002609 medium Substances 0.000 claims description 8
- 229920001817 Agar Polymers 0.000 claims description 7
- 239000008272 agar Substances 0.000 claims description 7
- 208000012839 conversion disease Diseases 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 7
- 229960003487 xylose Drugs 0.000 claims description 7
- XZIIFPSPUDAGJM-UHFFFAOYSA-N 6-chloro-2-n,2-n-diethylpyrimidine-2,4-diamine Chemical compound CCN(CC)C1=NC(N)=CC(Cl)=N1 XZIIFPSPUDAGJM-UHFFFAOYSA-N 0.000 claims description 6
- 239000001888 Peptone Substances 0.000 claims description 6
- 108010080698 Peptones Proteins 0.000 claims description 6
- 240000008042 Zea mays Species 0.000 claims description 6
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 6
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 6
- SRBFZHDQGSBBOR-LECHCGJUSA-N alpha-D-xylose Chemical compound O[C@@H]1CO[C@H](O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-LECHCGJUSA-N 0.000 claims description 6
- 239000000872 buffer Substances 0.000 claims description 6
- 229940041514 candida albicans extract Drugs 0.000 claims description 6
- 235000005822 corn Nutrition 0.000 claims description 6
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 claims description 6
- 238000003810 ethyl acetate extraction Methods 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 235000011187 glycerol Nutrition 0.000 claims description 6
- 239000001963 growth medium Substances 0.000 claims description 6
- 235000019319 peptone Nutrition 0.000 claims description 6
- 239000002953 phosphate buffered saline Substances 0.000 claims description 6
- 230000035484 reaction time Effects 0.000 claims description 6
- 238000011218 seed culture Methods 0.000 claims description 6
- 229940035044 sorbitan monolaurate Drugs 0.000 claims description 6
- 239000012138 yeast extract Substances 0.000 claims description 6
- 239000004744 fabric Substances 0.000 claims description 5
- 238000006555 catalytic reaction Methods 0.000 abstract description 11
- 239000008363 phosphate buffer Substances 0.000 abstract 1
- 238000002474 experimental method Methods 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 4
- 238000012807 shake-flask culturing Methods 0.000 description 4
- IMNFDUFMRHMDMM-UHFFFAOYSA-N N-Heptane Chemical compound CCCCCCC IMNFDUFMRHMDMM-UHFFFAOYSA-N 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 230000001629 suppression Effects 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 229920000742 Cotton Polymers 0.000 description 2
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 2
- 101000925662 Enterobacteria phage PRD1 Endolysin Proteins 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000003905 agrochemical Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000003197 catalytic effect Effects 0.000 description 2
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- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- 238000011914 asymmetric synthesis Methods 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a method for directly catalytically producing chiral (2S,3R)-1,2-(hexylidenedioxyl)heptyl-6-ene-3-ol by using candida norvegensis cell. The method comprises: adding a phosphate buffer into a reaction tank, adding ramie gauze to regulate and control the concentration of the substrate and the product, and using the yeast cell to perform biological catalytic reaction. The method is high in product yield and high in enantiomer excess, and has extremely good application value.
Description
Technical field
The invention belongs to technology of pharmaceutical engineering field, be related specifically to candida norvegensis cell biocatalysis and prepare chiral medicinal intermediate (2S, 3R)-1,2-(cyclohexylidene dioxy base) heptan-6-alkene-3-alcohol technology.
Background technology
Chirality (cyclohexylidene dioxy base)-6-alkene-3-in heptan alcohol is the important intermediate of synthesis of chiral medicine, fine chemicals, agricultural chemicals product and other exotic materialss.The asymmetry catalysis of biocatalysis has environmental friendliness, mild condition, selectivity advantages of higher, prepares the first approach of chipal compounds as green high-efficient, is applied to the chipal compounds producing some high added values more and more.Biocatalysis is prepared chirality (cyclohexylidene dioxy base)-6-alkene-3-in heptan alcohol and is had good application prospect.
Biocatalysis has the outstanding advantages such as catalytic efficiency is high, selectivity strong, mild condition, environmental friendliness, is the important method substituting and expand traditional organic chemical synthesis in process of sustainable development.Wherein chiral separation and asymmetric synthesis are the Application Areass of biocatalysis most magnetism.As in six large fermentoids of biological catalyst, lytic enzyme catalytic kinetics resolution of racemates can obtain quiral products, in industrial biocatalytic, play key player always.In recent years, oxydo-reductase application industrially obtained and increased rapidly.At present, the ratio adopting the industry of lytic enzyme Kinetic Resolution, biological reducing method and biological oxidation process to prepare optical activity chirality compound is 4:2:1.
(2S, 3R)-1,2-(cyclohexylidene dioxy base) heptan-6-alkene-3-alcohol be the important chiral building block of synthesis of chiral medicine, fine chemicals, agricultural chemicals product, owing to there being 2 chiral centres, make chemosynthesis comparatively difficult, cost is high.The present invention will adopt biocatalysis to prepare (2S, 3R)-1,2-(cyclohexylidene dioxy base) heptan-6-alkene-3-alcohol.
Summary of the invention
The present invention adopts candida norvegensis cell catalysis to prepare (2S, 3R)-1,2-(cyclohexylidene dioxy base) heptan-6-alkene-3-alcohol, reaction formula is as follows:
Substrate S-cyclohexylidene Glycerose (1) and the bromo-1-butylene of 4-(2), through candida norvegensis catalysis catalysis, obtain product (2S, 3R)-1,2-(cyclohexylidene dioxy base)-6-alkene-3-alcohol in heptan (3).Have multiple-microorganism can catalysis this reaction, through great many of experiments screening, finally determine adopt candida norvegensis as catalyzer because its catalyzed reaction
1 and 2 reactionseffect best, reaction yield, enantiomeric excess rate (ee%) are all very high.
Many candida norvegensis can carry out this reaction of biocatalysis, but its effect is different, differs greatly, and through experiment, the present invention selects candida norvegensis bacterial strain to be
aTCC 36586, its catalyzed reaction best results.
developing medium:
1, seed culture medium composition is: yeast extract 10 g/L, glucose 20 g/L, peptone 20 g/L, pH nature; The agar powder of 15g/L is added during preparation solid medium;
2, nutrient solution composition: glucose 25-30 g/L, glycerine 25-30 g/L, corn plumule powder 30-40 g/L, KH
2pO
49-11 g/L, MgSO
4.7H
2o 0.3-0.4 g/L, pH value is 6.0.
2, solid medium composition: add the agar powder of 2% in liquid medium within.
Prepared by yeast cell.Candida norvegensis through inclined-plane, shaking flask, seed tank culture obtain seed liquor; Fermentor tank adds nutrient solution, coefficient is 0.6-0.7,121 DEG C of autoclavings 30 minutes, be cooled to 30-31 DEG C, candida norvegensis seed liquor is seeded to fermentor tank, inoculative proportion is 8-10%, ventilation ratio is 0.9-1.2V/(V minute), cultivate 30-36 hour, obtain wet yeast cell, as biocatalytic reaction catalyzer with filtering centrifuge is centrifugal for 30-31 DEG C.
Because substrate, product all have restraining effect to yeast cell, in order to reduce the suppression of substrate, products upon cell, the present invention adopts ramie gauze immunoabsorbent substrate, in reaction, when substrate is by cell catalysis catalysis, when concentration reduces, substrate from the stripping of ramie gauze, postreaction consume substrate.Meanwhile, product is adsorbed by ramie gauze, decreases its concentration in water, thus considerably reduces the suppression of substrate, products upon cell.The ratio of substrate and ramie gauze, determines the concentration of substrate in reaction solution, product, and concentration is also relevant with temperature of reaction, reaction solution composition.Different cell is different to the susceptibility of substrate, product, so, great many of experiments be carried out, best substrate and the ratio of ramie gauze could be determined.Experiment shows, for bacterial strain of the present invention, reaction solution composition, temperature of reaction, best substrate and the ratio of ramie gauze are 0.36-0.38.During concrete absorption, by substrate S-cyclohexylidene Glycerose and the bromo-1-butylene of 4-miscible, R-cyclohexylidene Glycerose and the bromo-1-butylene mol ratio of 4-are 1, absorb this solution, namely obtain the ramie gauze having adsorbed substrate with the ramie gauze being of a size of 0.5cmX0.5cm.Regulate substrate and ramie gauze consumption, make the quality ratio of substrate and ramie gauze be 0.36-0.38.Ramie gauze used is common ramie gauze, and commercially, be cut into 0.5cmX0.5cm fritter, sterilizing, Preservation in sterile condition is stand-by.
Phosphate buffered saline buffer is added in bottom ventilation stirred tank, coefficient is 0.6-0.7, pH is 6.5, add the cotton gauze having adsorbed substrate S-cyclohexylidene Glycerose and the bromo-1-butylene of 4-, substrate S-cyclohexylidene Glycerose addition is made to be 80-90 g/L, substrate S-cyclohexylidene Glycerose and the bromo-1-butylene mol ratio of substrate 4-are 1, and 2 kinds of concentration of substrate all determine thus.Glucose content is 20-25g/L, wood sugar 13-15 g/L, and sorbitan monolaurate content is 19-20g/L, 121 DEG C of autoclavings 30 minutes; When being cooled to 27-28 DEG C, add wet yeast cell and make concentration be 22-26g/L, ventilation ratio is 0.15-0.18V/(V minute), namely per minute air flow is 0.15-0.18 times of reaction solution volume, carries out biocatalytic reaction, and the reaction times is 86-92 hour; After reaction terminates, leach cell, ramie gauze respectively, be extracted with ethyl acetate reaction solution, extraction ramie gauze, the ethyl acetate of combined ethyl acetate extraction liquid and extraction ramie gauze, steams ethyl acetate, obtain product (2S, 3R)-1,2-(cyclohexylidene dioxy base) heptan-6-alkene-3-alcohol, reaction conversion ratio 94-96%, product yield 92-94%, enantiomeric excess rate (ee%) 97-98%.
The present invention carries out the work and comprises bacterial strain selection (selecting from more or less a hundred bacterial strain), catalytic reaction condition optimizes (temperature of reaction, air flow, pH), reaction medium is selected and concentration optimization (concentration of substrate, glucose content, Xylose Content, kinds of surfactants (more than 20, kind selects 1) and concentration, other multiple components is selected to get rid of), solid absorption is adopted to control substrate product concentration, thus the suppression reduced cell, test carclazyte, diatomite, cotton gauze, ramie gauze, the many kinds of solids materials such as macropore resin, also once carried out the test of water-organic solvent 2 phase system.Because tested number is very large, although have employed response surface optimization design experiment, drastically reduce the area tested number, tested number is still very large, and total Test carries out just completing more than 2 years, reaches current technical scheme.For photolytic activity product, as reaction conversion ratio 94-96%, during product yield 92-94%, enantiomeric excess rate (ee%), still up to 97-98%, is very not easily, and our work achieves marked improvement.
embodiment 1
Seed culture medium composition is: yeast extract 10 g/L, glucose 20 g/L, peptone 20 g/L, pH nature; The agar powder of 15g/L is added during preparation solid medium;
Nutrient solution composition is: glucose 25 g/L, glycerine 25 g/L, corn plumule powder 30g/L, KH
2pO
49 g/L, MgSO
4.7H
2o 0.3g/L, pH value is 6.0.
Wet yeast cell preparation process is as follows: candida norvegensis ATCC 36586 through inclined-plane, shake-flask culture, obtain seed liquor.10L fermentor tank adds nutrient solution; Fermentor tank adds nutrient solution, coefficient is 0.6,121 DEG C of autoclavings 30 minutes, are cooled to 30 DEG C, candida norvegensis seed liquor are seeded to fermentor tank, inoculative proportion is 8%, ventilation ratio is 0.5V/(V minute), namely per minute air flow is 0.5 times of reaction solution volume, cultivates 30 hours for 30-31 DEG C, wet yeast cell is obtained, as biocatalytic reaction catalyzer with filtering centrifuge is centrifugal.
The ramie yarn cloth making method of having adsorbed substrate is, by substrate S-cyclohexylidene Glycerose and the bromo-1-butylene of 4-miscible, R-cyclohexylidene Glycerose and the bromo-1-butylene mol ratio of 4-are 1, this solution is absorbed with the ramie gauze being of a size of 0.5cmX0.5cm, namely the ramie gauze having adsorbed substrate is obtained, regulate substrate and ramie gauze consumption, make the quality ratio of substrate and ramie gauze be 0.36.Ramie gauze used is common ramie gauze, and commercially, be cut into 0.5cmX0.5cm fritter, sterilizing, Preservation in sterile condition is stand-by, and other embodiment ramie gauze process is identical.
Phosphate buffered saline buffer is added in 20L bottom ventilation stirred tank, coefficient is 0.6, pH is 6.5, add adsorbed substrate S-cyclohexylidene Glycerose and the bromo-1-butylene of 4-ramie gauze make substrate S-cyclohexylidene Glycerose addition be 80 g/L, substrate S-cyclohexylidene Glycerose and the bromo-1-butylene mol ratio of substrate 4-are 1, and glucose content is 20g/L, wood sugar 13 g/L, sorbitan monolaurate content is 19g/L, 121 DEG C of autoclavings 30 minutes; When being cooled to 27 DEG C, add wet yeast cell and make concentration be 22g/L, ventilation ratio is 0.15V/(V minute), carry out biocatalytic reaction, the reaction times is 86 hours; After reaction terminates, leach cell, ramie gauze respectively, be extracted with ethyl acetate reaction solution, extraction ramie gauze, the ethyl acetate of combined ethyl acetate extraction liquid and extraction ramie gauze, steams ethyl acetate, obtain product (2S, 3R)-1,2-(cyclohexylidene dioxy base) heptan-6-alkene-3-alcohol, reaction conversion ratio 94%, product yield 92%, enantiomeric excess rate (ee%) 97%.
embodiment 2
Seed culture medium composition is: yeast extract 10 g/L, glucose 20 g/L, peptone 20 g/L, pH nature; The agar powder of 15g/L is added during preparation solid medium;
Nutrient solution composition is: glucose 30 g/L, glycerine 30 g/L, corn plumule powder 40 g/L, KH
2pO
411 g/L, MgSO
4.7H
2o 0.4 g/L, pH value is 6.0.
Wet yeast cell preparation process is as follows: candida norvegensis ATCC 36586 through inclined-plane, shake-flask culture, obtain seed liquor.50L fermentor tank adds nutrient solution; Fermentor tank adds nutrient solution, coefficient is 0.7,121 DEG C of autoclavings 30 minutes, are cooled to 31 DEG C, candida norvegensis seed liquor are seeded to fermentor tank, inoculative proportion is 10%, ventilation ratio is 0.8V/(V minute), namely per minute air flow is 0.8 times of reaction solution volume, cultivates 36 hours for 31 DEG C, wet yeast cell is obtained, as biocatalytic reaction catalyzer with filtering centrifuge is centrifugal.
The ramie yarn cloth making method of having adsorbed substrate is, by substrate S-cyclohexylidene Glycerose and the bromo-1-butylene of 4-miscible, S-cyclohexylidene Glycerose and the bromo-1-butylene mol ratio of 4-are 1, this solution is absorbed with the ramie gauze being of a size of 0.5cmX0.5cm, namely the ramie gauze having adsorbed substrate is obtained, regulate substrate and ramie gauze consumption, make the quality ratio of substrate and ramie gauze be 0.38.
Phosphate buffered saline buffer is added in 100L bottom ventilation stirred tank, coefficient is 0.7, pH is 6.5, add adsorbed substrate S-cyclohexylidene Glycerose and the bromo-1-butylene of 4-ramie gauze make substrate S-cyclohexylidene Glycerose addition be 90 g/L, substrate S-cyclohexylidene Glycerose and the bromo-1-butylene mol ratio of substrate 4-are 1, and glucose content is 25g/L, wood sugar 15 g/L, sorbitan monolaurate content is 20g/L, 121 DEG C of autoclavings 30 minutes; When being cooled to 28 DEG C, add wet yeast cell and make concentration be 26g/L, ventilation ratio is 0.18V/(V minute), carry out biocatalytic reaction, the reaction times is 92 hours; After reaction terminates, leach cell, ramie gauze respectively, be extracted with ethyl acetate reaction solution, extraction ramie gauze, the ethyl acetate of combined ethyl acetate extraction liquid and extraction ramie gauze, steams ethyl acetate, obtain product (2S, 3R)-1,2-(cyclohexylidene dioxy base) heptan-6-alkene-3-alcohol, reaction conversion ratio 96%, product yield 94%, enantiomeric excess rate (ee%) 97%.
embodiment 3
Seed culture medium composition is: yeast extract 10 g/L, glucose 20 g/L, peptone 20 g/L, pH nature; The agar powder of 15g/L is added during preparation solid medium;
Nutrient solution composition is: glucose 28 g/L, glycerine 27 g/L, corn plumule powder 35 g/L, KH
2pO
410 g/L, MgSO
4.7H
2o 0.35 g/L, pH value is 6.0.
Wet yeast cell preparation process is as follows: candida norvegensis ATCC 36586 through inclined-plane, shake-flask culture, obtain seed liquor.10L fermentor tank adds nutrient solution; Fermentor tank adds nutrient solution, coefficient is 0.65,121 DEG C of autoclavings 30 minutes, are cooled to 30.5 DEG C, candida norvegensis seed liquor are seeded to fermentor tank, inoculative proportion is 9%, ventilation ratio is 0.65V/(V minute), namely per minute air flow is 0.65 times of reaction solution volume, cultivates 33 hours for 30.5 DEG C, wet yeast cell is obtained, as biocatalytic reaction catalyzer with filtering centrifuge is centrifugal.
The ramie yarn cloth making method of having adsorbed substrate is, by substrate S-cyclohexylidene Glycerose and the bromo-1-butylene of 4-miscible, R-cyclohexylidene Glycerose and the bromo-1-butylene mol ratio of 4-are 1, this solution is absorbed with the ramie gauze being of a size of 0.5cmX0.5cm, namely the ramie gauze having adsorbed substrate is obtained, regulate substrate and ramie gauze consumption, make the quality ratio of substrate and ramie gauze be 0.37.Ramie gauze used is common ramie gauze, and commercially, be cut into 0.5cmX0.5cm fritter, sterilizing, Preservation in sterile condition is stand-by, and other embodiment ramie gauze process is identical.
Phosphate buffered saline buffer is added in 15L bottom ventilation stirred tank, coefficient is 0.65, pH is 6.5, add adsorbed substrate S-cyclohexylidene Glycerose and the bromo-1-butylene of 4-ramie gauze make substrate S-cyclohexylidene Glycerose addition be 85 g/L, substrate S-cyclohexylidene Glycerose and the bromo-1-butylene mol ratio of substrate 4-are 1, and glucose content is 22g/L, wood sugar 14 g/L, sorbitan monolaurate content is 19.5g/L, 121 DEG C of autoclavings 30 minutes; When being cooled to 27.5 DEG C, add wet yeast cell and make concentration be 24g/L, ventilation ratio is 0.17V/(V minute), carry out biocatalytic reaction, the reaction times is 90 hours; After reaction terminates, leach cell, ramie gauze respectively, be extracted with ethyl acetate reaction solution, extraction ramie gauze, the ethyl acetate of combined ethyl acetate extraction liquid and extraction ramie gauze, steams ethyl acetate, obtain product (2S, 3R)-1,2-(cyclohexylidene dioxy base) heptan-6-alkene-3-alcohol, reaction conversion ratio 95%, product yield 93%, enantiomeric excess rate (ee%) 97.5%.
embodiment 4
Seed culture medium composition is: yeast extract 10 g/L, glucose 20 g/L, peptone 20 g/L, pH nature; The agar powder of 15g/L is added during preparation solid medium;
Nutrient solution composition is: glucose 27 g/L, glycerine 27 g/L, corn plumule powder 36g/L, KH
2pO
49.5 g/L, MgSO
4.7H
2o 0.3.7 g/L, pH value is 6.0.
Wet yeast cell preparation process is as follows: candida norvegensis ATCC 36586 through inclined-plane, shake-flask culture, obtain seed liquor.1000L fermentor tank adds nutrient solution; Fermentor tank adds nutrient solution, coefficient is 0.7,121 DEG C of autoclavings 30 minutes, are cooled to 31 DEG C, candida norvegensis seed liquor are seeded to fermentor tank, inoculative proportion is 10%, ventilation ratio is 0.8V/(V minute), namely per minute air flow is-0.8 times of reaction solution volume, cultivates 36 hours for 31 DEG C, wet yeast cell is obtained, as biocatalytic reaction catalyzer with filtering centrifuge is centrifugal.
The ramie yarn cloth making method of having adsorbed substrate is, by substrate S-cyclohexylidene Glycerose and the bromo-1-butylene of 4-miscible, R-cyclohexylidene Glycerose and the bromo-1-butylene mol ratio of 4-are 1, this solution is absorbed with the ramie gauze being of a size of 0.5cmX0.5cm, namely the ramie gauze having adsorbed substrate is obtained, regulate substrate and ramie gauze consumption, make the quality ratio of substrate and ramie gauze be 0.38.
Phosphate buffered saline buffer is added in 5000L bottom ventilation stirred tank, coefficient is 0.7, pH is 6.5, add adsorbed substrate S-cyclohexylidene Glycerose and the bromo-1-butylene of 4-ramie gauze make substrate S-cyclohexylidene Glycerose addition be 90 g/L, substrate S-cyclohexylidene Glycerose and the bromo-1-butylene mol ratio of substrate 4-are 1, and glucose content is 25g/L, wood sugar 15g/L, sorbitan monolaurate content is 20g/L, 121 DEG C of autoclavings 30 minutes; When being cooled to 28 DEG C, add wet yeast cell and make concentration be 26g/L, ventilation ratio is 0.17V/(V minute), carry out biocatalytic reaction, the reaction times is 91 hours; After reaction terminates, leach cell, ramie gauze respectively, be extracted with ethyl acetate reaction solution, extraction ramie gauze, the ethyl acetate of combined ethyl acetate extraction liquid and extraction ramie gauze, steams ethyl acetate, obtain product (2S, 3R)-1,2-(cyclohexylidene dioxy base) heptan-6-alkene-3-alcohol, reaction conversion ratio 96%, product yield 94%, enantiomeric excess rate (ee%) 98%.
Claims (4)
1. a candida norvegensis cell biocatalysis preparation (2S, 3R)-1, 2-(cyclohexylidene dioxy base) heptan-6-alkene-3-alcohol method, it is characterized in that adding phosphate buffered saline buffer in retort, coefficient is 0.6-0.7, pH is 6.5, add the ramie gauze having adsorbed substrate R-cyclohexylidene Glycerose and the bromo-1-butylene of 4-, substrate R-cyclohexylidene Glycerose addition is made to be 80-90 g/L, substrate R-cyclohexylidene Glycerose and the bromo-1-butylene mol ratio of substrate 4-are 1, glucose content is 20-25g/L, wood sugar 13-15 g/L, sorbitan monolaurate content is 19-20g/L, 121 DEG C of autoclavings 30 minutes, when being cooled to 27-28 DEG C, add wet yeast cell and make concentration be 22-26g/L, ventilation ratio is 0.15-0.18V/(V minute), carry out biocatalytic reaction, the reaction times is 86-92 hour, after reaction terminates, leach cell, ramie gauze respectively, be extracted with ethyl acetate reaction solution, extraction ramie gauze, the ethyl acetate of combined ethyl acetate extraction liquid and extraction ramie gauze, steams ethyl acetate, obtain product (2S, 3R)-1,2-(cyclohexylidene dioxy base) heptan-6-alkene-3-alcohol, reaction conversion ratio 94-96%, product yield 92-94%, enantiomeric excess rate (ee%) 97-98%.
2. method according to claim 1, is characterized in that wet yeast cell preparation process is as follows: candida norvegensis through inclined-plane, shaking flask, seed tank culture obtain seed liquor; Fermentor tank adds nutrient solution, coefficient is 0.6-0.7,121 DEG C of autoclavings 30 minutes, be cooled to 30-31 DEG C, candida norvegensis seed liquor is seeded to fermentor tank, inoculative proportion is 8-10%, ventilation ratio is 0.5-0.8V/(V minute), cultivate 30-36 hour, obtain wet yeast cell, as biocatalytic reaction catalyzer with filtering centrifuge is centrifugal for 30-31 DEG C.
3. method according to claim 1, is characterized in that seed culture medium composition is: yeast extract 10 g/L, glucose 20 g/L, peptone 20 g/L, pH nature; The agar powder of 15g/L is added during preparation solid medium;
Nutrient solution composition is: glucose 25-30 g/L, glycerine 25-30 g/L, corn plumule powder 30-40 g/L, KH
2pO
49-11 g/L, MgSO
4.7H
2o 0.3-0.4 g/L, pH value is 6.0.
4. method according to claim 1, it is characterized in that described ramie yarn cloth making method of having adsorbed substrate is, by substrate R-cyclohexylidene Glycerose and the bromo-1-butylene of 4-miscible, R-cyclohexylidene Glycerose and the bromo-1-butylene mol ratio of 4-are 1, this solution is absorbed with the ramie gauze being of a size of 0.5cmX0.5cm, namely obtain the ramie gauze having adsorbed substrate, regulate substrate and ramie gauze consumption, make the quality ratio of substrate and ramie gauze be 0.36-0.38.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005082559A (en) * | 2003-09-10 | 2005-03-31 | National Institute Of Agrobiological Sciences | Optically active compound having 3-oxabicycl[3.3.0]octane skeleton and its use |
| CN1793355A (en) * | 2005-12-01 | 2006-06-28 | 华东理工大学 | Organic solvent slow-releasing system and its preparation and application for catalyzing reaction of enzyme thereof |
| US7465842B2 (en) * | 2004-08-26 | 2008-12-16 | Agouron Pharmaceuticals, Inc. | Enantioselective biotransformation for preparation of protein tyrosine kinase inhibitor intermediates |
-
2014
- 2014-10-13 CN CN201410537233.5A patent/CN104328153A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005082559A (en) * | 2003-09-10 | 2005-03-31 | National Institute Of Agrobiological Sciences | Optically active compound having 3-oxabicycl[3.3.0]octane skeleton and its use |
| US7465842B2 (en) * | 2004-08-26 | 2008-12-16 | Agouron Pharmaceuticals, Inc. | Enantioselective biotransformation for preparation of protein tyrosine kinase inhibitor intermediates |
| CN1793355A (en) * | 2005-12-01 | 2006-06-28 | 华东理工大学 | Organic solvent slow-releasing system and its preparation and application for catalyzing reaction of enzyme thereof |
Non-Patent Citations (1)
| Title |
|---|
| MIYU FURUTA等: "Stereoselective approach to (2R,3S)- and (2R,3R)-1,2-(cyclohexylidenedioxy)hept-6-en-3-ol by microbial reduction", 《JOURNAL OF MOLECULAR CATALYSIS B: ENZYMATIC》 * |
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