CN104328074A - Microalgae cultivation method under low illumination condition - Google Patents
Microalgae cultivation method under low illumination condition Download PDFInfo
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- CN104328074A CN104328074A CN201410610407.6A CN201410610407A CN104328074A CN 104328074 A CN104328074 A CN 104328074A CN 201410610407 A CN201410610407 A CN 201410610407A CN 104328074 A CN104328074 A CN 104328074A
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Abstract
The invention relates to a microalgae cultivation technology. Particularly, in a microalgae autotrophic cultivation process, a chlorophyll fluorescence kinetic parameter Fv/Fm is used as a judgment criterion and the microalgae Fv/Fm is reduced by 0-10% to be a threshold value under a low ventilation capacity; a lowest night oxygen introduction amount is determined so that a biomass loss generated by the night respiratory action of microalgae is reduced; and the accumulation of microalgae biomasses is promoted. The ventilation capacity is reduced so that the energy consumption of ventilation is reduced and the accumulation efficiency of the biomasses is improved.
Description
Technical field:
The invention belongs to a kind of micro-algae high efficiency breeding technology.
Background technology:
Algae classifies as autotrophic type microorganism, because there is photosynthesizer PSII in its cell also referred to as micro-algae plant.Micro-algae has efficient absorption sun power, fixation of C O
2produce the ability (i.e. photosynthetic capacity) of biomass; Simultaneously micro-algae is as the one of microorganism, its rate of propagation very fast (doubling time be about 3-5 days, the fastest 8 hours).As unicellular organism, micro-algae mainly consist of albumen, fat, carbohydrate and nucleic acid etc.Microalgae grease content is different because of algae kind, is generally 20-30%, reaches as high as 60-70%.Micro-algae fixes greenhouse gases CO as one
2produce the raw material that bio-oil provides biofuel, be day by day subject to the extensive attention of scientific and technological circle and industry member.
As photosynthetic organism organism, micro-algae has that biomass is large, growth cycle is short, easy cultivation and containing features such as higher lipids, is CO
2reduce discharging, prepare the important renewable resources of biofuel and small molecule hydrocarbon.Utilize micro-algae to prepare biofuel and there are four features:
(1) Solar use efficiency is high, individuality is little is unicellular or filament, and diameter is less than 1mm.High-density can be carried out in photoreactor, two-forty is cultivated.Under similarity condition, the microalgae cell growth doubling time, the logarithmic phase inner cell material doubling time can foreshorten to 3.5h usually in 24h.
(2) fat content of micro-algae enriches, and some algae such as fat content of Wild Vitis species can reach 80% of dry weight;
(3) micro-algae grows in water, and culture area is little, and floor space is very little and can not take agricultural land compared with traditional oil crops, and therefore algae bio Energy production is not striven with production estimation and ploughed and strive grain with people.
(4) micro-algae can utilize GHG carbon dioxide to produce bioenergy, is conducive to ecological environmental protection, promotes the construction of harmony between man and nature society.A large amount of greenhouse gases CO is discharged in mineral substance energy process of consumption
2, toxic gas NO
xand SO
xdeng.The current CO of China
2total emission volumn be only second to the U.S., occupy second place of the world, along with China becomes a full member of Kyoto Protocol, CO
2the pressure reduced discharging is by increasing.Consume in the face of solving China's mineral substance energy the great demand causing environmental destruction in a large number, micro-algae can fix a large amount of CO
2, production process is polluted few simultaneously.
The above-mentioned application of micro-algae, its core is exactly efficient photosynthetic carbon fixation.In culturing process by analysis natural lighting (or the change of simulating nature periodicity of illumination), when unglazed photograph, micro-algae needs to provide the energy of surviving and required for growth by respiration, and this is that consumption biomass discharge CO simultaneously only
2process.As in the Dark-light cycle of 14:10 micro-algae autotrophy culturing process, lose due to respiratory consumption without the even more biomass of photophase 15-20%.Therefore, the raising of microdisk electrode photosynthetic carbon fixation efficiency, except needing to improve in photostage, also needs to optimize the culture condition without photophase, makes whole efficiency the highest.
In microdisk electrode process, in order to improve mass-transfer efficiency, mostly mix by passing into air, in air lift type and bubbling style reactor, the power resources of air or overall circulation.Passing into also for system introduces oxygen of air, and be excessive oxygen mostly, facilitate respiratory generation.If do not pass into oxygen, at unglazed cultivation stage, cell owing to lacking hybrid power sedimentation, dark fermentation have accumulated a large amount of Toxic Metabolites and lacks effective physiology and prepare to cause the cessation of growth cessation at illumination initial stage to increase, will to photostage biomass accumulation have a negative impact and even cultivate failure.
Therefore, if possible effective without reducing passing into of oxygen photostage, and to cells survival, growth without obvious negative effect, just likely reduce the biomass loss and CO that produce because of respiration
2release.But, due to the dynamic change of the condition such as micro algae biomass, nutrition supply in culture systems, if the reliable index of neither one, be difficult to the minimum oxygen intake determining " without negative impact ".
Micro-algae is as ptotoautotroph, the same with plant, the physiological parameter of its photosynthetical system---chlorophyll fluorescence kinetics parameters F
v/ F
mit is the whether normal index of cell, degree [the reference: YAO C H whether microalgae cell is in stress state and coerces can be judged by the change of this value, AI J N, CAO X P, et al.Enhancing starch production of a marine green microalga Tetraselmis subcordiformis through nutrient limitation.Bioresource Technology, 2012,118:438-444.].
Based on above-mentioned analysis, the present invention proposes first with microalgae cell photosynthetical system chlorophyll fluorescence parameters F
v/ F
mfor instruction, by reducing the intake without oxygen in photostage, controlling respiration and being in low-level, and realizing biomass consumption and CO
2the reduction of release, improves the optical energy utilization efficiency of overall culturing process.Meanwhile, the reduction of air flow also will reduce the energy consumption of micro-algae autotrophy culturing process.
Summary of the invention
1, in micro-algae autotrophy culturing process of normal Dark-light cycle (as green alga, chrysophyceae, diatom and spirulina), under comprising natural lighting or artificial lighting condition, with the Change of Chlorophyll Fluorescence Kinetics Parameters F of chlorophyll fluorescence instrument to micro-algae photosynthetical system II
v/ F
mmonitor, regulate the oxygen intake without photostage.According to different culture process, pass into gas and refer to air, or oxygen volume content is nitrogen, the oxygen mixture of 21%.Use air, reducing oxygen intake can directly by reducing the amount realization passing into air to culture system; Use nitrogen oxygen atmosphere, reducing oxygen intake can pass into the amount of gas mixture to culture system by reducing or reduce the ratio realization of oxygen in gas mixture.
2, for different microalgae, with the F under illumination condition
v/ F
mfor benchmark, take 5-10% as threshold value, namely as the F of frustule after reduction oxygen-supply quantity
v/ F
mcompared with normal ventilatory conditions decline 5-10% is standard, determines required minimum oxygen intake.
Tool of the present invention has the following advantages:
(1) with chlorophyll fluorescence kinetics parameters F
v/ F
mfor index, carry out effectively determining reliably and optimizing to regulation and control parameter;
(2) by reducing without amount in ventilation in photostage (oxygen), part suppresses micro-algae respiration, decreases because breathing the biomass loss and extra CO that cause
2release;
(3) while raising biomass production efficiency, the energy consumption without ventilation in photostage is reduced.
Accompanying drawing explanation
Under Fig. 1, typical light secretly change culture condition, the Changing Pattern of microalgae biomass.
Inventive principle:
Chlorophyll fluorescence kinetics parameters F
v/ F
mbe a parameter of the intrinsic ability characterizing photosynthetical system II, can be used for describing the degree of coercing suffered by microalgae cell.Reduce oxygen concn, will the aerobic repiration of microalgae cell be unfavorable for, and certain coercing can be produced to frustule; In order to obtain higher production efficiency, needing frustule can grow ready for photostage, namely without keeping necessary physiologically active photostage, just can utilize F
v/ F
mbalance both the sign of micro-algae stress level is realized.
Embodiment:
Embodiment 1,
Under standard 3x F/2 culture medium condition, according to 14:10 Light To Dark Ratio, lsochrysis zhanjiangensis is cultivated in 500mL tubular type bioreactor, wherein the normal illumination stage pass into 100mL/min containing volume ratio 2%CO
2air, without photostage, the air passing into same flow velocity (does not additionally supplement CO
2).During mensuration different time points, biomass (dry weight, mg/ml), the results are shown in Figure 1.Result shows, in end point in low photostage, the biomass decline 15-20% of the relatively high illumination end point of total biomass in culture systems, namely the respiration etc. in low photostage result in the loss of 15-20% biomass.
Embodiment 2,
According to culture condition described in embodiment 1, unglazed 50% (50mL/min) and 20% (20mL/min) being adjusted to normal level according to section air intake respectively, measures unglazed section of shining and starts and F during end point
v/ F
mchange ratio, compare normal level, 50% and 20% group of decline respectively 1.0% and 7.5%, determine that the air flow of 20% is minimum air flow.
Therefore, with unglazed 20% (20mL/min) being adjusted to normal level according to section air flow, measure unglazed section of shining and start the change ratio with biomass during end point.Result shows, low air flow biomass content 0.423g/L, and the 0.389g/L height about 8.7% of the normal ventilation group comparatively contrasted, reduces the loss of biomass.
Claims (5)
1. the microalgae culture method under low-light conditions, is characterized in that:
Under low-light conditions, by reducing the amount passing into oxygen in culture system, under maintenance cell normal physiological activity prerequisite, the biomass loss suppressing micro-algae to produce because of respiration, promotes the accumulation of microalgae biomass.
2., according to method described in claim 1, it is characterized in that:
Described low-light conditions is that the input light intensity of microdisk electrode system is less than or equal to 10 μMs of m
-2s
-1.
3., according to method described in claim 1, it is characterized in that:
The described gas that passes into refers to air, or oxygen volume content is nitrogen, the oxygen mixture of 21%;
Use air, reducing oxygen intake can directly by reducing the amount realization passing into air to culture system; Use nitrogen oxygen atmosphere, reducing oxygen intake can pass into the amount of gas mixture to culture system by reducing or reduce the ratio realization of oxygen in gas mixture.
4., according to method described in claim 1 or 2, it is characterized in that:
Describedly to refer under maintenance cell normal physiological activity prerequisite, utilize the chlorophyll fluorescence kinetics parameters F characterizing micro-algae photosynthetical system two activity
v/ F
mfor criterion; With under low-light conditions, in culture system, pass into the parameter F during oxygen of normal amount
v/ F
mvalue is benchmark, reduces the amount passing into oxygen in culture system gradually, with micro-algae F
v/ F
mrelative decline 5-10% is threshold value, i.e. parameter F
v/ F
mwhen value is reduced to the 90-95% of benchmark, be defined as required oxygen intake.
5., according to method described in claim 1, it is characterized in that: described micro-algae refer to can carry out autotrophy cultivation green alga, chrysophyceae, diatom or spirulina.
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108132233A (en) * | 2016-12-01 | 2018-06-08 | 中国科学院大连化学物理研究所 | A kind of microalgae nutritional supplementation control method based on cell light-use level |
CN110157620A (en) * | 2019-04-18 | 2019-08-23 | 厦门大学 | A kind of cultural method improving purple ball algae synthesis phycoerythrin content |
CN113607909A (en) * | 2021-08-09 | 2021-11-05 | 中国科学院合肥物质科学研究院 | Method for constructing water body comprehensive toxicity characterization parameters by algae photosynthetic inhibition method |
CN114431131A (en) * | 2022-01-07 | 2022-05-06 | 中国科学院东北地理与农业生态研究所 | Aeration culture method for improving survival rate of algae |
CN116024093A (en) * | 2023-03-27 | 2023-04-28 | 中国科学院海洋研究所 | Light supply method in dunaliella salina culture process and application |
Citations (1)
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CN103911290A (en) * | 2014-04-16 | 2014-07-09 | 沈阳化工研究院有限公司 | Close-type microalgae culture method by intermittent gas introduction in dark period |
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CN103911290A (en) * | 2014-04-16 | 2014-07-09 | 沈阳化工研究院有限公司 | Close-type microalgae culture method by intermittent gas introduction in dark period |
Non-Patent Citations (1)
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Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108132233A (en) * | 2016-12-01 | 2018-06-08 | 中国科学院大连化学物理研究所 | A kind of microalgae nutritional supplementation control method based on cell light-use level |
CN110157620A (en) * | 2019-04-18 | 2019-08-23 | 厦门大学 | A kind of cultural method improving purple ball algae synthesis phycoerythrin content |
CN110157620B (en) * | 2019-04-18 | 2021-03-30 | 厦门大学 | Culture method for improving content of phycoerythrin synthesized by porphyridium |
CN113607909A (en) * | 2021-08-09 | 2021-11-05 | 中国科学院合肥物质科学研究院 | Method for constructing water body comprehensive toxicity characterization parameters by algae photosynthetic inhibition method |
CN113607909B (en) * | 2021-08-09 | 2023-11-07 | 中国科学院合肥物质科学研究院 | Construction method of comprehensive toxicity characterization parameters of water body by algae photosynthesis inhibition method |
CN114431131A (en) * | 2022-01-07 | 2022-05-06 | 中国科学院东北地理与农业生态研究所 | Aeration culture method for improving survival rate of algae |
CN116024093A (en) * | 2023-03-27 | 2023-04-28 | 中国科学院海洋研究所 | Light supply method in dunaliella salina culture process and application |
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Application publication date: 20150204 |