CN104328074A - Microalgae cultivation method under low illumination condition - Google Patents

Microalgae cultivation method under low illumination condition Download PDF

Info

Publication number
CN104328074A
CN104328074A CN201410610407.6A CN201410610407A CN104328074A CN 104328074 A CN104328074 A CN 104328074A CN 201410610407 A CN201410610407 A CN 201410610407A CN 104328074 A CN104328074 A CN 104328074A
Authority
CN
China
Prior art keywords
oxygen
algae
micro
microalgae
reducing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201410610407.6A
Other languages
Chinese (zh)
Inventor
薛松
曹旭鹏
褚亚东
陆洪斌
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Dalian Institute of Chemical Physics of CAS
Original Assignee
Dalian Institute of Chemical Physics of CAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dalian Institute of Chemical Physics of CAS filed Critical Dalian Institute of Chemical Physics of CAS
Priority to CN201410610407.6A priority Critical patent/CN104328074A/en
Publication of CN104328074A publication Critical patent/CN104328074A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor

Landscapes

  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention relates to a microalgae cultivation technology. Particularly, in a microalgae autotrophic cultivation process, a chlorophyll fluorescence kinetic parameter Fv/Fm is used as a judgment criterion and the microalgae Fv/Fm is reduced by 0-10% to be a threshold value under a low ventilation capacity; a lowest night oxygen introduction amount is determined so that a biomass loss generated by the night respiratory action of microalgae is reduced; and the accumulation of microalgae biomasses is promoted. The ventilation capacity is reduced so that the energy consumption of ventilation is reduced and the accumulation efficiency of the biomasses is improved.

Description

Microalgae culture method under a kind of low-light conditions
Technical field:
The invention belongs to a kind of micro-algae high efficiency breeding technology.
Background technology:
Algae classifies as autotrophic type microorganism, because there is photosynthesizer PSII in its cell also referred to as micro-algae plant.Micro-algae has efficient absorption sun power, fixation of C O 2produce the ability (i.e. photosynthetic capacity) of biomass; Simultaneously micro-algae is as the one of microorganism, its rate of propagation very fast (doubling time be about 3-5 days, the fastest 8 hours).As unicellular organism, micro-algae mainly consist of albumen, fat, carbohydrate and nucleic acid etc.Microalgae grease content is different because of algae kind, is generally 20-30%, reaches as high as 60-70%.Micro-algae fixes greenhouse gases CO as one 2produce the raw material that bio-oil provides biofuel, be day by day subject to the extensive attention of scientific and technological circle and industry member.
As photosynthetic organism organism, micro-algae has that biomass is large, growth cycle is short, easy cultivation and containing features such as higher lipids, is CO 2reduce discharging, prepare the important renewable resources of biofuel and small molecule hydrocarbon.Utilize micro-algae to prepare biofuel and there are four features:
(1) Solar use efficiency is high, individuality is little is unicellular or filament, and diameter is less than 1mm.High-density can be carried out in photoreactor, two-forty is cultivated.Under similarity condition, the microalgae cell growth doubling time, the logarithmic phase inner cell material doubling time can foreshorten to 3.5h usually in 24h.
(2) fat content of micro-algae enriches, and some algae such as fat content of Wild Vitis species can reach 80% of dry weight;
(3) micro-algae grows in water, and culture area is little, and floor space is very little and can not take agricultural land compared with traditional oil crops, and therefore algae bio Energy production is not striven with production estimation and ploughed and strive grain with people.
(4) micro-algae can utilize GHG carbon dioxide to produce bioenergy, is conducive to ecological environmental protection, promotes the construction of harmony between man and nature society.A large amount of greenhouse gases CO is discharged in mineral substance energy process of consumption 2, toxic gas NO xand SO xdeng.The current CO of China 2total emission volumn be only second to the U.S., occupy second place of the world, along with China becomes a full member of Kyoto Protocol, CO 2the pressure reduced discharging is by increasing.Consume in the face of solving China's mineral substance energy the great demand causing environmental destruction in a large number, micro-algae can fix a large amount of CO 2, production process is polluted few simultaneously.
The above-mentioned application of micro-algae, its core is exactly efficient photosynthetic carbon fixation.In culturing process by analysis natural lighting (or the change of simulating nature periodicity of illumination), when unglazed photograph, micro-algae needs to provide the energy of surviving and required for growth by respiration, and this is that consumption biomass discharge CO simultaneously only 2process.As in the Dark-light cycle of 14:10 micro-algae autotrophy culturing process, lose due to respiratory consumption without the even more biomass of photophase 15-20%.Therefore, the raising of microdisk electrode photosynthetic carbon fixation efficiency, except needing to improve in photostage, also needs to optimize the culture condition without photophase, makes whole efficiency the highest.
In microdisk electrode process, in order to improve mass-transfer efficiency, mostly mix by passing into air, in air lift type and bubbling style reactor, the power resources of air or overall circulation.Passing into also for system introduces oxygen of air, and be excessive oxygen mostly, facilitate respiratory generation.If do not pass into oxygen, at unglazed cultivation stage, cell owing to lacking hybrid power sedimentation, dark fermentation have accumulated a large amount of Toxic Metabolites and lacks effective physiology and prepare to cause the cessation of growth cessation at illumination initial stage to increase, will to photostage biomass accumulation have a negative impact and even cultivate failure.
Therefore, if possible effective without reducing passing into of oxygen photostage, and to cells survival, growth without obvious negative effect, just likely reduce the biomass loss and CO that produce because of respiration 2release.But, due to the dynamic change of the condition such as micro algae biomass, nutrition supply in culture systems, if the reliable index of neither one, be difficult to the minimum oxygen intake determining " without negative impact ".
Micro-algae is as ptotoautotroph, the same with plant, the physiological parameter of its photosynthetical system---chlorophyll fluorescence kinetics parameters F v/ F mit is the whether normal index of cell, degree [the reference: YAO C H whether microalgae cell is in stress state and coerces can be judged by the change of this value, AI J N, CAO X P, et al.Enhancing starch production of a marine green microalga Tetraselmis subcordiformis through nutrient limitation.Bioresource Technology, 2012,118:438-444.].
Based on above-mentioned analysis, the present invention proposes first with microalgae cell photosynthetical system chlorophyll fluorescence parameters F v/ F mfor instruction, by reducing the intake without oxygen in photostage, controlling respiration and being in low-level, and realizing biomass consumption and CO 2the reduction of release, improves the optical energy utilization efficiency of overall culturing process.Meanwhile, the reduction of air flow also will reduce the energy consumption of micro-algae autotrophy culturing process.
Summary of the invention
1, in micro-algae autotrophy culturing process of normal Dark-light cycle (as green alga, chrysophyceae, diatom and spirulina), under comprising natural lighting or artificial lighting condition, with the Change of Chlorophyll Fluorescence Kinetics Parameters F of chlorophyll fluorescence instrument to micro-algae photosynthetical system II v/ F mmonitor, regulate the oxygen intake without photostage.According to different culture process, pass into gas and refer to air, or oxygen volume content is nitrogen, the oxygen mixture of 21%.Use air, reducing oxygen intake can directly by reducing the amount realization passing into air to culture system; Use nitrogen oxygen atmosphere, reducing oxygen intake can pass into the amount of gas mixture to culture system by reducing or reduce the ratio realization of oxygen in gas mixture.
2, for different microalgae, with the F under illumination condition v/ F mfor benchmark, take 5-10% as threshold value, namely as the F of frustule after reduction oxygen-supply quantity v/ F mcompared with normal ventilatory conditions decline 5-10% is standard, determines required minimum oxygen intake.
Tool of the present invention has the following advantages:
(1) with chlorophyll fluorescence kinetics parameters F v/ F mfor index, carry out effectively determining reliably and optimizing to regulation and control parameter;
(2) by reducing without amount in ventilation in photostage (oxygen), part suppresses micro-algae respiration, decreases because breathing the biomass loss and extra CO that cause 2release;
(3) while raising biomass production efficiency, the energy consumption without ventilation in photostage is reduced.
Accompanying drawing explanation
Under Fig. 1, typical light secretly change culture condition, the Changing Pattern of microalgae biomass.
Inventive principle:
Chlorophyll fluorescence kinetics parameters F v/ F mbe a parameter of the intrinsic ability characterizing photosynthetical system II, can be used for describing the degree of coercing suffered by microalgae cell.Reduce oxygen concn, will the aerobic repiration of microalgae cell be unfavorable for, and certain coercing can be produced to frustule; In order to obtain higher production efficiency, needing frustule can grow ready for photostage, namely without keeping necessary physiologically active photostage, just can utilize F v/ F mbalance both the sign of micro-algae stress level is realized.
Embodiment:
Embodiment 1,
Under standard 3x F/2 culture medium condition, according to 14:10 Light To Dark Ratio, lsochrysis zhanjiangensis is cultivated in 500mL tubular type bioreactor, wherein the normal illumination stage pass into 100mL/min containing volume ratio 2%CO 2air, without photostage, the air passing into same flow velocity (does not additionally supplement CO 2).During mensuration different time points, biomass (dry weight, mg/ml), the results are shown in Figure 1.Result shows, in end point in low photostage, the biomass decline 15-20% of the relatively high illumination end point of total biomass in culture systems, namely the respiration etc. in low photostage result in the loss of 15-20% biomass.
Embodiment 2,
According to culture condition described in embodiment 1, unglazed 50% (50mL/min) and 20% (20mL/min) being adjusted to normal level according to section air intake respectively, measures unglazed section of shining and starts and F during end point v/ F mchange ratio, compare normal level, 50% and 20% group of decline respectively 1.0% and 7.5%, determine that the air flow of 20% is minimum air flow.
Therefore, with unglazed 20% (20mL/min) being adjusted to normal level according to section air flow, measure unglazed section of shining and start the change ratio with biomass during end point.Result shows, low air flow biomass content 0.423g/L, and the 0.389g/L height about 8.7% of the normal ventilation group comparatively contrasted, reduces the loss of biomass.

Claims (5)

1. the microalgae culture method under low-light conditions, is characterized in that:
Under low-light conditions, by reducing the amount passing into oxygen in culture system, under maintenance cell normal physiological activity prerequisite, the biomass loss suppressing micro-algae to produce because of respiration, promotes the accumulation of microalgae biomass.
2., according to method described in claim 1, it is characterized in that:
Described low-light conditions is that the input light intensity of microdisk electrode system is less than or equal to 10 μMs of m -2s -1.
3., according to method described in claim 1, it is characterized in that:
The described gas that passes into refers to air, or oxygen volume content is nitrogen, the oxygen mixture of 21%;
Use air, reducing oxygen intake can directly by reducing the amount realization passing into air to culture system; Use nitrogen oxygen atmosphere, reducing oxygen intake can pass into the amount of gas mixture to culture system by reducing or reduce the ratio realization of oxygen in gas mixture.
4., according to method described in claim 1 or 2, it is characterized in that:
Describedly to refer under maintenance cell normal physiological activity prerequisite, utilize the chlorophyll fluorescence kinetics parameters F characterizing micro-algae photosynthetical system two activity v/ F mfor criterion; With under low-light conditions, in culture system, pass into the parameter F during oxygen of normal amount v/ F mvalue is benchmark, reduces the amount passing into oxygen in culture system gradually, with micro-algae F v/ F mrelative decline 5-10% is threshold value, i.e. parameter F v/ F mwhen value is reduced to the 90-95% of benchmark, be defined as required oxygen intake.
5., according to method described in claim 1, it is characterized in that: described micro-algae refer to can carry out autotrophy cultivation green alga, chrysophyceae, diatom or spirulina.
CN201410610407.6A 2014-10-31 2014-10-31 Microalgae cultivation method under low illumination condition Pending CN104328074A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201410610407.6A CN104328074A (en) 2014-10-31 2014-10-31 Microalgae cultivation method under low illumination condition

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201410610407.6A CN104328074A (en) 2014-10-31 2014-10-31 Microalgae cultivation method under low illumination condition

Publications (1)

Publication Number Publication Date
CN104328074A true CN104328074A (en) 2015-02-04

Family

ID=52402886

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201410610407.6A Pending CN104328074A (en) 2014-10-31 2014-10-31 Microalgae cultivation method under low illumination condition

Country Status (1)

Country Link
CN (1) CN104328074A (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108132233A (en) * 2016-12-01 2018-06-08 中国科学院大连化学物理研究所 A kind of microalgae nutritional supplementation control method based on cell light-use level
CN110157620A (en) * 2019-04-18 2019-08-23 厦门大学 A kind of cultural method improving purple ball algae synthesis phycoerythrin content
CN113607909A (en) * 2021-08-09 2021-11-05 中国科学院合肥物质科学研究院 Method for constructing water body comprehensive toxicity characterization parameters by algae photosynthetic inhibition method
CN114431131A (en) * 2022-01-07 2022-05-06 中国科学院东北地理与农业生态研究所 Aeration culture method for improving survival rate of algae
CN116024093A (en) * 2023-03-27 2023-04-28 中国科学院海洋研究所 Light supply method in dunaliella salina culture process and application

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103911290A (en) * 2014-04-16 2014-07-09 沈阳化工研究院有限公司 Close-type microalgae culture method by intermittent gas introduction in dark period

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103911290A (en) * 2014-04-16 2014-07-09 沈阳化工研究院有限公司 Close-type microalgae culture method by intermittent gas introduction in dark period

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
张恒: "基于套管式新型光生物反应器的微藻培养实验系统设计及初步调试", 《中国优秀硕士学位论文全文数据库 基础科学辑》 *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108132233A (en) * 2016-12-01 2018-06-08 中国科学院大连化学物理研究所 A kind of microalgae nutritional supplementation control method based on cell light-use level
CN110157620A (en) * 2019-04-18 2019-08-23 厦门大学 A kind of cultural method improving purple ball algae synthesis phycoerythrin content
CN110157620B (en) * 2019-04-18 2021-03-30 厦门大学 Culture method for improving content of phycoerythrin synthesized by porphyridium
CN113607909A (en) * 2021-08-09 2021-11-05 中国科学院合肥物质科学研究院 Method for constructing water body comprehensive toxicity characterization parameters by algae photosynthetic inhibition method
CN113607909B (en) * 2021-08-09 2023-11-07 中国科学院合肥物质科学研究院 Construction method of comprehensive toxicity characterization parameters of water body by algae photosynthesis inhibition method
CN114431131A (en) * 2022-01-07 2022-05-06 中国科学院东北地理与农业生态研究所 Aeration culture method for improving survival rate of algae
CN116024093A (en) * 2023-03-27 2023-04-28 中国科学院海洋研究所 Light supply method in dunaliella salina culture process and application

Similar Documents

Publication Publication Date Title
CN103805514B (en) A kind ofly photosynthetic the holding concurrently of inorganic nitrogen-sourced micro-algae is utilized to support high density fermentation cultural method and application
Gao et al. A novel algal biofilm membrane photobioreactor for attached microalgae growth and nutrients removal from secondary effluent
Rodolfi et al. Microalgae for oil: Strain selection, induction of lipid synthesis and outdoor mass cultivation in a low‐cost photobioreactor
Ji et al. Biofilm cultivation of the oleaginous microalgae Pseudochlorococcum sp.
Ono et al. Carbon dioxide mitigation using thermophilic cyanobacteria
Pires et al. Carbon dioxide capture from flue gases using microalgae: engineering aspects and biorefinery concept
CN104328074A (en) Microalgae cultivation method under low illumination condition
CN105648023B (en) A kind of method that microalgae mixed culture prepares grease
CN102311920B (en) Culture method for chlorella
Znad et al. CO2 biomitigation and biofuel production using microalgae: photobioreactors developments and future directions
CN104450525A (en) Combined method for microalgae breeding and denitration of exhaust gas
CN107460216A (en) A kind of method that microalgae grease is produced using flue gas
CN106032522B (en) A kind of combined method of cultivating microalgae and industrial waste gas denitration
TWI433647B (en) Microalgae cultivation module
CN113105991B (en) Micro-nano bubble culture and recovery integrated microalgae biofilm reactor and microalgae culture and recovery method thereof
CN105713934B (en) A method of producing microalgae grease
CN104232559B (en) The method of cultivating microalgae and the method for producing grease
CN105713935B (en) A kind of method of microalgae mixed culture production grease
CN107699493B (en) Microalgae cultivation method
CN105713951B (en) Method for preparing microalgae grease
CN113692962B (en) Carbon-fixing microalgae cultivation method
CN103305425B (en) A kind of method being improved Botryococcus braunii hydrocarbon output by Illumination adjusting
CN102492625B (en) Microalga composite culture solution, preparation method and application thereof
CN112899125B (en) Microalgae efficient carbon sequestration device and nutrient supplement control method
CN104946536A (en) Isochrysis zhangjiangensis culture method

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20150204