CN104327934B - A kind of refinement method of barley worm grease - Google Patents
A kind of refinement method of barley worm grease Download PDFInfo
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- CN104327934B CN104327934B CN201410258905.9A CN201410258905A CN104327934B CN 104327934 B CN104327934 B CN 104327934B CN 201410258905 A CN201410258905 A CN 201410258905A CN 104327934 B CN104327934 B CN 104327934B
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/025—Pretreatment by enzymes or microorganisms, living or dead
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/001—Refining fats or fatty oils by a combination of two or more of the means hereafter
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/02—Refining fats or fatty oils by chemical reaction
- C11B3/06—Refining fats or fatty oils by chemical reaction with bases
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/10—Refining fats or fatty oils by adsorption
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- General Chemical & Material Sciences (AREA)
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- Edible Oils And Fats (AREA)
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Abstract
A kind of refinement method of barley worm grease of the present invention, belongs to insects technical field of agricultural product process；There is provided that a kind of yield height, safe green, product quality are high, do not destroy its useful component, and the extracting method that accessory substance can be used；Including making underflow, enzymolysis, centrifugation, extraction and refining, feature is：The centrifugation comprises the following steps：After solution is inactivated after enzymolysis, in rotating speed in more than 3500r/min centrifugal treatings, centrifugation time is in more than 15min；Upper strata oily, emulsion liquid are taken out with pipettor, demulsification more than 30min under less than 18 DEG C environment is placed on；By the liquid after demulsification in centrifugal rotational speed in more than 3500r/min, the time centrifuges again in more than 10min；Present invention is mainly applied in terms of the extraction of barley worm grease.
Description
Technical field
A kind of refinement method of barley worm grease of the present invention, belongs to insects technical field of agricultural product process.
Background technology
The super bread worm of barley worm alias, high protein worm.Because its feeding habits is miscellaneous, wide adaptability, feed resource extensively and cost
It is cheap, raised extensively in China each department, its yield is higher by five than yellow meal worm, six times, survival rate is far higher by yellow meal worm, up to
95%.Barley worm larva body length is up to 6cm or so, larva average out to containing protein 51% or so, fatty average out to 29% or so,
And containing various saccharides, amino acid, vitamin, hormone, enzyme and mineral phosphor, iron, potassium, sodium, calcium etc., nutritive value considerably beyond
The insects such as fly maggot, earthworm, yellow meal worm and cricket, the content of its protein and grease etc. is in first of all kinds of living insects, is had
The features such as growth is fast, breeding coefficient is high.Still it is in the junior stage for the research of barley worm at present, to the research of its deep processing more
It is to be in blank, its value needs to be developed.On barley worm grease, its current extraction process is still in squeezing, You Jirong
The more coarse operation stage such as agent extraction, its danger coefficient is big, recovery rate is low, product qualities are low, needs a kind of new technology badly
Improve production.
The content of the invention
In order to overcome the shortcomings of that prior art is present there is provided a kind of yield height, safe green, product quality is high, do not destroy
Its useful component, and the extracting method that accessory substance can be used.
In order to solve the above-mentioned technical problem, the technical solution adopted by the present invention is：A kind of refinement method of barley worm grease,
Including making underflow, enzymolysis, centrifugation, extraction and refining, the centrifugation comprises the following steps：
After solution is inactivated after enzymolysis, in rotating speed in more than 3500r/min centrifugal treatings, centrifugation time 15min with
On；
Upper strata oily, emulsion liquid are taken out with pipettor, demulsification more than 30min under less than -18 DEG C environment is placed on；
By the liquid after demulsification in centrifugal rotational speed in more than 3500r/min, the time centrifuges again in more than 10min.
The preferred scheme of the centrifugation is：After solution is inactivated after enzymolysis, in rotating speed at more than 4000r/min centrifugations
Reason, centrifugation time is in 15min；
Upper strata oily, emulsion liquid are taken out with pipettor, the 30min that is demulsified is placed under -18 DEG C of environment；
By the liquid after demulsification in centrifugal rotational speed in 4000r/min, the time is that 10min is centrifuged again.
The making underflow comprises the following steps：
Fresh barley worm is chosen, by its washes clean；
Then barley worm is dried into 10h at 60 DEG C, be milled afterwards, sieved with 40 mesh sieve；
Finally the barley worm powder obtained in previous step is mixed with water by 5-6: 1.
The enzymolysis comprises the following steps：
Underflow is stirred in constant temperature blender with magnetic force and is warming up to 50-60 DEG C；
Then PH to 8.0-9.0 is adjusted；
Alkali protease, trypsase and bromelain are added, it is 1: 1: 0.1 that it, which is matched, and it is 600U/g with enzyme amount,
Its enzymolysis time is 100-150min, and its hydrolysis temperature is 50-60 DEG C.
Described refining is first water law degumming, then alkali-refining deacidification, and last adsorbent decolourizes.
Solution after enzymolysis is placed at 95 DEG C and inactivates 10min, then is centrifuged.
The extraction refers to take the upper strata oily liquids after centrifugation.
The solid-liquid ratio of barley worm powder and water during making underflow is 5: 1.
Stirring underflow in constant temperature blender with magnetic force in affiliated enzymolysis process is warming up to 55 DEG C；
Then PH to 8.5 is adjusted；
Alkali protease, trypsase and bromelain are added, it is 1: 1: 0.1 that it, which is matched, and its consumption is respectively 600U/
g：600U/g：60U/g, its enzymolysis time is 120min, and its hydrolysis temperature is 55 DEG C.
Described water law degumming is to heat the grease of extraction, adds distilled water and mixes, upper strata grease is taken after centrifugation；
The alkali-refining deacidification is then centrifuged for add alkaline solution in the upper strata grease after degumming, then takes upper strata grease, is stirred adding water
Centrifugation is mixed, until waste water is finally dehydrated in neutrality；Grease is is heated and keeps constant temperature by the charcoal absorption, while stirring
Mix side adsorbent and be divided into 5 batches and be gradually added in grease, be warming up to 100 DEG C, constant temperature stirring, suction filtration obtains the grease of clear.
The present invention have the advantage that compared with prior art for：
The color and luster for the barley worm oil that the present invention is extracted is faint yellow, is better than the extraction of petroleum ether；Peroxide value and acid number
It is the important indicator for evaluating oil quality, its oily acid number of the barley worm that the present invention is extracted and peroxide value are respectively less than petroleum ether and carried
Take, show to comprise only a small amount of free fatty in oil, the degree very little of oxidative rancidity occurs for resulting grease, makes in grease
Nutritional ingredient obtained preferable reservation.The present invention relies primarily in mechanical agitation and enzymolysis, extraction and does not need organic solvent,
The gas of pollution environment will not be produced, the inflammable and explosive problem that also solvent-free extraction is easily produced in taking；Aqueous enzymatic method proposes oily effect
Mild condition, no high-temperature process can be good at the oily quality for ensureing to extract, some ANFs during oil is carried
It can be also removed with the poisonous factor, while low energy consumption and consumption can be reduced again without excessive subsequent treatment, it is cost-effective.
Brief description of the drawings
The embodiment of the present invention is described in further detail below by accompanying drawing.
Fig. 1 is influence of the enzymolysis time to oil yield；
Fig. 2 is influence of the enzyme dosage to oil yield；
Fig. 3 is influence of the solid-liquid ratio to oil yield；
Fig. 4 is influence of the hydrolysis temperature to oil yield；
Influences of the Fig. 5 for enzymolysis PH to oil yield；
Fig. 6 is factor level table；
Fig. 7 is orthogonal experiment data analysis table；
Fig. 8 is process chart.
Embodiment
Below by embodiment, the present invention is further detailed explanation.
First, material and instrument
Main material：Barley worm larva；Trypsase；Alkali protease；Bromelain；Absolute ether；Formaldehyde, hydrogen-oxygen
Change sodium, hydrochloric acid (being domestic AR)；Activated carbon, atlapulgite etc..
Key instrument：PB203-N precise electronics balance, DECTA320pH meters (plum Teller-support benefit instrument company)；HH-6
Digital display thermostat water bath (Guo Hua Electric companies)；SHB-III multiplex vavuum pump of circulating water type (Zhengzhou Great Wall instrument plant)；80-2B
Desk centrifuge (Anting Scientific Instrument Factory, Shanghai)；WD850CMG-5578MTW micro-wave ovens (LG ELECTRONICS Co., Ltd)；88-1
Type time constant-temperature magnetic stirring apparatus (Shanghai Si Le instrument plants)；Baking oven；Pulverizer；Soxhlet extractor is a set of：Wide-mouth bottle, degreasing
Line, absorbent cotton, degreasing fine sand.
2nd, experimental procedure
1st, oil yield is determined
Oil yield (%)=A/ (B × C) × 100
In formula：Barley worm oil quality/g that A- is extracted；
Quality/g of B- barley worms；
Mass fraction/% of crude fat in C- barley worms.
The measure of crude fat：With reference to GB/T5512-1985.
Extractor is installed appropriate, the filtration paper cylinder that then will be equipped with sample is placed in extracting barrel, while injecting ether to rainbow
More than suction pipe height, after ether stream is net, ether is added at 2/3rds of siphon pipe height.It is light with a fritter absorbent cotton
Lightly fill in condenser pipe suitable for reading, open condenser pipe water inlet pipe, begin to warm up extracting.The temperature of heating is with the ether of backflow per minute
In 12~50 drops, flow back more than seven times per hour.The time of extracting must extract depending on sample oil content, typically in more than 8h
Untill the ether of extraction tube third is with sheet glass inspection (drop experiment) oil stains-less.
Pump after fat, filtration paper cylinder is taken out with long handle tweezers, reheating makes aether backflow 2 times, then withdraws ether, remove
Condenser pipe and extracting barrel, heating eliminate ether remaining in extracting bottle, dip in ether with absorbent cotton and wipe outside net extracting bottle, then will
Bottle elder generation A90min. 20min again at a temperature of 105 DEG C are extracted, are dried to untill plant is weighed that (front and rear secondary weight difference is within 0.00028
It is considered as value weight).The extracting increased weight of bottle is the weight of crude fat.
Crude fat wet basis content, contents on dry basis and the miscellaneous lower content of standard water press formula respectively
In formula：W- crude fat weight, g
W- style weight, g
M- sample percent water rates, %
M- sample standard moisture, standard impurities sum, %
2nd, technological process
Fig. 8 is shown in technological process.
3rd, barley worm grease is extracted specific experiment operation and method
1st, single factor experiment
(1) influence of the enzymolysis time to oil yield
Selection liquid ratio is that 55 DEG C of 5: 1, temperature, enzyme concentration 600U/g raw materials, pH8.0, mixing speed are 200r/min, plus
The ratio between alkali protease, trypsase and bromelain enzyme activity for entering were 1: 1: 0.1 (similarly hereinafter), and enzymolysis time is selected respectively
30th, 60,90,120,150,180min is digested.
As shown in figure 1, with the gradually increase of enzymolysis time, the recovery rate of barley worm oil constantly increases, and works as enzymolysis time
After 120min, oil extracting rate tends towards stability.This method carries time of oil because of the Bu Tong difference of species and the raw material of enzyme, it is determined that
During enzymolysis time, the factors such as yield, the recovery rate of oil and the production cost of protein should be considered.Enzymolysis time is short, then
Raw material is not sufficiently reactive, and grease does not discharge completely, if overlong time, and the emulsion formed can more be stablized and be difficult to brokenly
Breast, influences oil extracting rate.Consider, enzymolysis time selection 120min.
(2) influence of the enzyme dosage to oil yield
Trypsase, alkali protease, bromelain are added according to 1: 1: 0.1 ratio, selection liquid ratio is 5:
1st, 55 DEG C of temperature, pH8.0, enzymolysis time 120min, the mixing speed selection enzyme-added total amounts of 200r/min select 200 respectively, 400,
600th, 800,1000,1200U/g raw materials are digested.
As shown in Fig. 2 the recovery rate of barley worm oil is as the consumption of enzyme increases and constantly increases, when the consumption of enzyme reaches
The change of oil extracting rate tends towards stability during 600U/g raw materials.The species of enzyme is different with consumption, also differs for the action effect of raw material
Sample, the selectivity of enzyme determines that it can only work to specific composition in raw material.Therefore, it should according to raw material type and each group
Point content select the addition of suitable enzyme and enzyme.Consider production cost and oil extracting rate, suitable enzyme concentration is
600U/g raw materials.
(3) influence of the solid-liquid ratio to oil yield
Enzyme concentration 600U/g raw materials, pH8.0, temperature is selected to be selected for 55 DEG C, enzymolysis time selection 120min, mixing speed
200r/min, liquid ratio selection 3: 1,4: 1,5: 1,6: 1,7: 1 is digested.
As shown in figure 3, the recovery rate of barley worm oil is continuously increased with the increase of liquid ratio, grease when liquid ratio is 5: 1
Extraction rate reached to maximum, liquid ratio continue increase when, oil extracting rate reduction.In this method carries oil, liquid ratio should be as small as possible
Some, are so conducive to the separation of water and oil, while the generation of waste water can also be reduced.The addition of liquid is very such as in oil is carried
When many, the speed of reaction can be accelerated, but more water-oil emulsions and more waste water can be formed, subsequent treatment is increased
Cost.Consider oil extracting rate and cost, selection liquid ratio is 5: 1.
(4) influence of the hydrolysis temperature to oil yield
It is 5: 1, pH8.0, enzymolysis time 120min, mixing speed selection 200r/min, hydrolysis temperature point to select liquid ratio
Solution selection is 45,50,55,60,65 DEG C digested.
As shown in figure 4, with the rise of temperature, the recovery rate of barley worm oil constantly increases, when temperature reaches 55 DEG C, grease
Extraction rate reached to maximum.Continue to increase temperature, oil extracting rate reduction.Temperature is that this method puies forward a key factor during oil,
The selection of hydrolysis temperature will consider the species of enzyme and the difference of raw material, and selected temperature will be such that enzyme digestion reaction fully carries out, together
When should not also influence the albumen of generation and the quality of the product such as oil.The temperature of reaction is low, and enzymolysis speed is slow, influences oil extracting rate,
Temperature is too high, then can cause the activity reduction of enzyme, and the albumen of generation can be denatured, and the oily color and luster of extraction can shade, influence
The quality of product.Therefore, suitable temperature is 55 DEG C.
(5) influences of the enzymolysis pH to oil yield
Select liquid ratio be 5: 1,55 DEG C of temperature, enzymolysis time 120min, mixing speed selection 200r/min, enzymolysis
PH selects 7.0,7.5,8.0,8.5,9.0 to be digested respectively.
As shown in figure 5, the recovery rate of grease increases and increased with pH, when pH reaches 8.0, the recovery rate of barley worm oil is most
Greatly, when pH is 8.5, the recovery rate of grease slightly has reduction, continues to increase pH, oil extracting rate declines rapidly.During this method carries oil,
PH selection should be according to selected enzyme species, it not only influences enzyme activity, also has large effect for the separation of albumen.Albumen and
The yield of oil has close contact, and when oil extracting rate is higher, the yield of albumen also can be very high；In the recovery rate highest of albumen
During pH, oily yield also highest.Protein degree of hydrolysis near isoelectric point is minimum, and now oil extracting rate is also minimum.This method carries oily pH
Selection should consider the aspect factors of pH two of enzyme itself and protein extraction.Therefore, pH selections 8.0 are advisable.
2nd, Orthogonal Optimization Test
The result that summary experiment of single factor is drawn understands that enzymolysis time extracts the shadow of grease with liquid ratio to barley worm
Sound is limited to a certain extent, and the extraction of barley worm oil would not be produced after the two factors reach to a certain degree
Excessive influence, so we select liquid ratio, hydrolysis temperature, enzymolysis pH is used as three factors, factor level table such as Fig. 6 institutes
Show.
Orthogonal experiment results in the case of liquid ratio 5: 1, enzymolysis time 120min are as shown in Figure 7.
This experiment determines optimal barley worm hydrolysising condition by orthogonal experiment, experimental studies have found that, pH value is to barley worm water
The influence of solution is maximum, is secondly enzyme dosage, influence minimum is the temperature value digested.Optimal experiment is matched：It is enzyme-added
600U/g is measured, temperature is 55 DEG C, pH8.5.The secondary factors for the influence that trypsase is hydrolyzed to barley worm are：PH value>Enzyme concentration>
Temperature.The optimum extraction rate of grease is 76.5%.
4th, barley worm oil and fat refining
The refining process of barley worm grease includes water law degumming, three steps of alkali-refining deacidification and adsorption bleaching.
1st, water law degumming：
Grease will just be carried by heat to the distilled water mixing for after 85 DEG C, being rapidly added 4%85 DEG C, 30min is stirred.Afterwards
5000r/min centrifuges 10min, and upper strata is settled grease, and lower floor is a small amount of colloid.Take upper strata grease.
2nd, alkali-refining deacidification：
(1) measurement is by the acid number of the upper strata grease after degumming, and estimation adds the amount of NaOH solution, and it is 0.4% to make excess alkali quantity.
First oil is heated to 60 DEG C, NaOH solution is rapidly added, persistently stirs 1h, makes alkali lye and free-fat acid reaction abundant.Observation
Make to be brought rapidly up to 85 DEG C to tiny neat soap, stir 1h, 5000r/min centrifuges 10min afterwards.
(2) take upper strata grease to add 30%85 DEG C of water, continue to stir 30min, be placed in separatory funnel and separate.It is so continuous
Washing, until waste water is in neutrality.
(3) grease after washing is placed in 85 DEG C of environment and dehydrated.
3rd, adsorbent decolourizes：
(1) activating adsorbent：
The activation of activated carbon：In the hydrochloric acid that 100g activated carbons are added to 750mL1mol/L, water-bath boiling reflux 2h, while hot
Pumping filtering, with more than 90 DEG C of distilled water flushings to ferric ion is not contained, dries, is placed in drier afterwards.
The activation of atlapulgite：Carclazyte is placed in 150 DEG C of baking ovens and heats 2h, is placed on after cooling in drier.
(2) decolourize
The ratio of activated carbon and atlapulgite is 1: 10.Grease is heated to 85 DEG C and constant temperature is kept, while stirring by 3%
Adsorbent is divided into 5 batches and is gradually added in grease, is warming up to 100 DEG C, constant temperature stirring 20min, horse back suction filtration obtains clear
Grease.
5th, Analysis of conclusion
The water white transparency for the barley worm oil that this method is extracted, is better than organic solvent extraction.Peroxide value and acid number are to comment
The important indicator of valency oil quality, its oily acid number of barley worm and peroxide value that the method is extracted is far smaller than organic solvent extraction
Take, minimal amount of free fatty is comprised only in oil.Therefore, during this method carries oil, oxidizing acid occurs for resulting grease
The degree very little lost, makes the nutritional ingredient in grease obtain preferable reservation.The barley worm oil that extraction and this method are extracted
Iodine number is very close, without obvious difference.The barley worm oil extracted using organic solvent, its saponification number is than grease that this method is extracted
It is small, show to there may be some non-saponifying materials in oil, proposed together by organic solvent with oil during oil is carried.
This method as it is a kind of it is new carry oil tech, rely primarily on mechanical agitation, enzymolysis and refine, need not be organic in extraction
Solvent, will not produce pollution environment gas, also it is solvent-free extraction take in easily produce it is inflammable and explosive the problems such as；This method carries oil
Action condition is gentle, no high-temperature process, can be good at ensureing the quality of grease, some ANFs during oil is carried
It can be also removed with the poisonous factor.Low energy consumption simultaneously and consumption can be reduced again without excessive subsequent treatment, it is cost-effective.
Claims (8)
1. a kind of refinement method of barley worm grease, including make underflow, enzymolysis, centrifugation, extraction and refine, it is characterised in that：
The centrifugation comprises the following steps：
After solution is inactivated after enzymolysis, in rotating speed in more than 3500r/min centrifugal treatings, centrifugation time is in more than 15min；
Upper strata oily, emulsion liquid are taken out with pipettor, demulsification more than 30min under less than -18 DEG C environment is placed on；
By the liquid after demulsification in centrifugal rotational speed in more than 3500r/min, the time centrifuges again in more than 10min；
The enzymolysis comprises the following steps：
Underflow is stirred in constant temperature blender with magnetic force and is warming up to 50-60 DEG C；
Then PH to 8.0-9.0 is adjusted；
Alkali protease, trypsase and bromelain are added, it is 1: 1: 0.1 that it, which is matched, and it is 600U/g with enzyme amount, its enzyme
The solution time is 100-150min, and its hydrolysis temperature is 50-60 DEG C.
2. a kind of refinement method of barley worm grease according to claim 1, it is characterised in that：The preferred side of the centrifugation
Case is：After solution is inactivated after enzymolysis, in rotating speed in more than 4000r/min centrifugal treatings, centrifugation time is in 15min；
Upper strata oily, emulsion liquid are taken out with pipettor, the 30min that is demulsified is placed under -18 DEG C of environment；
By the liquid after demulsification in centrifugal rotational speed in 4000r/min, the time is that 10min is centrifuged again.
3. a kind of refinement method of barley worm grease according to claim 1, it is characterised in that：The making underflow includes
Following steps：
Fresh barley worm is chosen, by its washes clean；
Then barley worm is dried into 10h at 60 DEG C, be milled afterwards, sieved with 40 mesh sieve；
Finally the barley worm powder obtained in previous step is mixed with water by 5-6: 1.
4. a kind of refinement method of barley worm grease according to claim 1, it is characterised in that：Described refining is first water
Method degumming, then alkali-refining deacidification, last adsorbent decolourize.
5. a kind of refinement method of barley worm grease according to claim 1, it is characterised in that：Solution after enzymolysis is put
In inactivating 10min at 95 DEG C, then centrifuged.
6. a kind of refinement method of barley worm grease according to claim 3, it is characterised in that：The material of barley worm powder and water
Liquor ratio is 5: 1.
7. a kind of refinement method of barley worm grease according to claim 1, it is characterised in that：By underflow in constant temperature magnetic force
Stirring is warming up to 55 DEG C in agitator；
Then PH to 8.5 is adjusted；
Alkali protease, trypsase and bromelain are added, it is 1: 1: 0.1 that it, which is matched, and its consumption is respectively 600U/g:
600U/g: 60U/g, its enzymolysis time is 120min, and its hydrolysis temperature is 55 DEG C.
8. a kind of refinement method of barley worm grease according to claim 4, it is characterised in that：Described water law degumming is
The grease of extraction is heated, distilled water is added and mixes, upper strata grease is taken after centrifugation；The alkali-refining deacidification is after degumming
Alkaline solution is added in the grease of upper strata, is then centrifuged for, then takes upper strata grease, in the stirring centrifugation that adds water, until waste water is in neutrality, most
After dehydrate；The adsorbent decolourizes to heat grease and keep constant temperature, and adsorbent is divided into 5 batches and is gradually added while stirring
In grease, 100 DEG C are warming up to, constant temperature stirring, suction filtration obtains the grease of clear.
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| CN104327934A (en) | 2015-02-04 |
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