CN104316700A - 一种乳品多参数快速检测卡的制备方法 - Google Patents
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Abstract
本发明公开了一种乳品多参数快速检测卡的制备方法，属于乳制品检测技术领域。将吸水纸、玻璃纤维、聚酯纤维等材料浸泡于相应的制备液中，一定时间后烘干处理即得到相应的试纸。若再将各种试纸有间隔的固定于纸板、PVC、PET、带胶底板等硬质底板材料上，经过剪切即得到乳品多参数快速检测卡。本发明包含一个检测卡硬质底板以及多个单一元素快速检测试纸块。将一条乳品多参数快速检测卡浸泡至样液中，不同单一元素的试纸块会快速显色，根据相应元素的显色情况与比色卡一一进行对比，即可得到元素的检测数值。检测者在使用检测卡时无需对同一个乳品样品进行多次预处理，只需针对预测试的样品进行一次简单的预处理，便可用测试卡一次测试即得到多个参数的测试结果。
Description
技术领域
本发明属于食品检测技术领域，具体涉及一种乳品多参数快速检测卡的制备方法。
背景技术
目前市场上有很多厂家均有测试牛奶、奶粉等乳制品中单一参数的多种检测试纸产品，它们也能对乳品中单一的参数进行简便快捷的检测，如吉大小天鹅蛋白质快速检测试纸、昆明泊银科技有限公司的奶粉蛋白质检测试纸、泰宜康蛋白质检测试纸、智云达牛奶中尿素快速检测试纸、杭州陆恒生物技术有限公司的食品牛奶中过氧化氢试纸等，但是这些针对乳品进行检测的产品检测项目较为单一，不能使检测者更为清晰地了解预检测样品中各个指标的相关情况。此外，目前市场上还有一些试纸，如德国MN公司的锌测试条，用于电镀和金属加工业的检测，杭州陆恒生物技术有限公司的重金属锌检测试纸，用于测试饮用水、自来水、食品加工用水、工农业用水等水质，德国MN公司的钙镁离子检测试纸、杭州陆恒生物技术有限公司的钙镁总硬度检测试纸等均用来检测水质，这些市售的检测试纸没有针对乳品微量元素的检测产品。这些试纸均为将经过特殊处理的检测某种元素或物质的试纸固定于纸板、PVC等硬质底板材料上制成。也有一些专利是关于检测单独项目的试纸的，如US3511608、US4061468、US3802842等专利，但几乎没有仅针对乳品检测项目的试纸的专利。随着乳品近些年来出现的问题，如“大头奶粉”、三聚氰胺等，人们越来越关注，也更加重视乳品的质量和安全问题，尤其是婴幼儿奶粉出现的食品安全问题更加引起人们的重视，因此对于它们的监督和检测也显得更为重要，但是现有产品却尚不能满足人们对于乳品中，尤其是婴幼儿奶粉中各种营养元素、有害物质、非法添加物等物质的快速简便并同时检测多个参数的需求，同时，目前现有的一些产品操作不够简便，对于家庭用、非专业的人员使用都不够便捷。
发明内容
本发明提供一种乳品多参数快速检测卡的制备方法，检测者在使用检测卡时无需对同一个乳品样品进行多次预处理，只需针对预测试的样品进行一次简单的预处理，便可用测试卡一次测试即得到多个参数的测试结果。
一种乳品多参数快速检测卡的制备方法，制备步骤如下
第一步，分别制备蛋白质试纸的试剂、锌试纸的试剂、镁试纸的试剂、钙试纸的试剂、亚硝酸盐试纸的试剂、淀粉试纸的试剂和过氧化氢试纸的试剂；
所述蛋白质试纸的试剂配方为：柠檬酸8-15g，柠檬酸钠10-20g，溴酚蓝0.5-2g，曲拉通0.5-2g，吐温1-5g，纯净水或蒸馏水160-200mL；
所述锌试纸的试剂配方为：2-[5-溴-2-吡啶偶氮]-5-二乙氨基苯酚0.01-2g，柠檬酸钠20-35g，乙醇3-10mL，吐温0.1-5g，盐酸5-20mL，纯净水或蒸馏水120-180mL；
所述镁试纸的试剂配方为：钙镁试剂0.1-1g，氢氧化钠1-5g，氯化钠0.02-2g，三乙醇胺10-50mL，氢氧化钾1-5g，曲拉通1-10g，纯净水或蒸馏水120-180mL；
所述钙试纸的试剂配方为：偶氮胂I 0.1-1g，8羟基喹啉0.5-2g，硼酸钠0.2-5g，氢氧化钠1-8g，曲拉通1-10g，聚乙二醇1-5g，纯净水或蒸馏水200mL；
所述亚硝酸盐试纸的试剂配方为：0.2-3g盐酸萘乙二胺，10-50mL乙酸，对氨基苯磺酸0.5-4g，乙醇50-100mL，纯净水或蒸馏水50-150mL；
所述淀粉试纸的试剂配方为：1-4g碘化钾，0.2-2g碘，曲拉通1-5g，纯净水或蒸馏水200mL；
所述过氧化氢试纸的试剂配方为：四甲基联苯胺0.5-2g，辣根过氧化物酶0.002-0.02g，柠檬酸20-40g，柠檬酸钠15-40g，乙醇10-50mL，纯净水或蒸馏水60-150mL；
第二步：将大张的滤纸或者吸水纸等体积的浸入各种检测试纸的试剂中，全部浸透3-10分钟后立即送入30-80℃的烘箱中将其烘干；
第三步：将各种检测项目的试纸先剪切成条状，宽度控制在0.4-1厘米之间，有间隔地粘贴于底板上，再进行剪切，使每种检测试纸的尺寸控制在(0.4-1)厘米×(0.4-1)厘米之间，即得乳品多参数快速检测卡。
进一步的，所述过氧化氢试纸和亚硝酸盐试纸需低温避光保存。
有益效果：
1、本发明克服了检测多参数时需要对同一个样品进行多次预处理，从而会浪费较多样品的不足，检测者只需对一个样品进行最简单的预处理一次，即可同时测试出乳品样品中多个参数的相应数值。因此本发明可以实现对乳品最为简便、快捷、高效、准确的检测，又可节约样品，避免样品、耗材等的浪费，同时也可减少多次检测对环境造成的污染，此外对于家庭、非专业人员的操作也更容易实现。
2、本发明基于同一个样品进行多个参数的同时检测，包括营养物质、微量元素、有毒有害物质、掺假物质等。此试纸操作方便快捷，灵敏度更高，检测更准确。可以为工商、乳品商户、消费者等提供一个快速简便检测乳品质量的工具。若检测者需要检测乳品中的几种元素，用现有的一些产品需要先对样品进行不同的预处理，逐一地进行相应元素的检测，不同产品的检测时间和方法也不尽相同，因此可能需要逐一地进行相应元素的检测，这样的话需要十分钟甚至更长的时间才能完成几种元素的检测，得到检测结果。而用本发明只需多样品进行最简单的预处理一次，然后将乳品多参数快速检测卡浸入样液中几秒钟至3分钟，取出与比色卡对照，即可同时得到多个参数的数值，全部检测过程不会超过5分钟，因此与现有产品相比，更加的简便、快捷、节约、环保。
具体实施方式
本发明提供了一种乳品多参数快速检测卡的制备方法，制备步骤如下
第一步，分别制备蛋白质试纸的试剂、锌试纸的试剂、镁试纸的试剂、钙试纸的试剂、亚硝酸盐试纸的试剂、淀粉试纸的试剂和过氧化氢试纸的试剂；
所述蛋白质试纸的试剂配方为：柠檬酸8-15g，柠檬酸钠10-20g，溴酚蓝0.5-2g，曲拉通0.5-2g，吐温1-5g，纯净水或蒸馏水160-200mL；
所述锌试纸的试剂配方为：2-[5-溴-2-吡啶偶氮]-5-二乙氨基苯酚0.01-2g，柠檬酸钠20-35g，乙醇3-10mL，吐温0.1-5g，盐酸5-20mL，纯净水或蒸馏水120-180mL；
所述镁试纸的试剂配方为：钙镁试剂0.1-1g，氢氧化钠1-5g，氯化钠0.02-2g，三乙醇胺10-50mL，氢氧化钾1-5g，曲拉通1-10g，纯净水或蒸馏水120-180mL；
所述钙试纸的试剂配方为：偶氮胂I 0.1-1g，8羟基喹啉0.5-2g，硼酸钠0.2-5g，氢氧化钠1-8g，曲拉通1-10g，聚乙二醇1-5g，纯净水或蒸馏水200mL；
所述亚硝酸盐试纸的试剂配方为：0.2-3g盐酸萘乙二胺，10-50mL乙酸，对氨基苯磺酸0.5-4g，乙醇50-100mL，纯净水或蒸馏水50-150mL；
所述淀粉试纸的试剂配方为：1-4g碘化钾，0.2-2g碘，曲拉通1-5g，纯净水或蒸馏水200mL；
所述过氧化氢试纸的试剂配方为：四甲基联苯胺0.5-2g，辣根过氧化物酶0.002-0.02g，柠檬酸20-40g，柠檬酸钠15-40g，乙醇10-50mL，纯净水或蒸馏水60-150mL；
第二步：将大张的滤纸或者吸水纸等体积的浸入各种检测试纸的试剂中，全部浸透3-10分钟后立即送入30-80℃的烘箱中将其烘干；
第三步：将各种检测项目的试纸先剪切成条状，宽度控制在0.4-1厘米之间，有间隔地粘贴于底板上，再进行剪切，使每种检测试纸的尺寸控制在(0.4-1)厘米×(0.4-1)厘米之间，即得乳品多参数快速检测卡。
进一步的，所述过氧化氢试纸和亚硝酸盐试纸需低温避光保存。
检测时，将一条乳品多参数快速检测卡浸泡至样液中，不同单一元素的试纸块会快速显色，根据相应元素的显色情况与比色卡一一进行对比，即可得到元素的检测数值。
综上所述，以上仅为本发明的较佳实施例而已，并非用于限定本发明的保护范围。凡在本发明的精神和原则之内，所作的任何修改、等同替换、改进等，均应包含在本发明的保护范围之内。
Claims (2)
1.一种乳品多参数快速检测卡的制备方法，其特征在于，制备步骤如下
第一步，分别制备蛋白质试纸的试剂、锌试纸的试剂、镁试纸的试剂、钙试纸的试剂、亚硝酸盐试纸的试剂、淀粉试纸的试剂和过氧化氢试纸的试剂；
所述蛋白质试纸的试剂配方为：柠檬酸8-15g，柠檬酸钠10-20g，溴酚蓝0.5-2g，曲拉通0.5-2g，吐温1-5g，纯净水或蒸馏水160-200mL；
所述锌试纸的试剂配方为：2-[5-溴-2-吡啶偶氮]-5-二乙氨基苯酚0.01-2g，柠檬酸钠20-35g，乙醇3-10mL，吐温0.1-5g，盐酸5-20mL，纯净水或蒸馏水120-180mL；
所述镁试纸的试剂配方为：钙镁试剂0.1-1g，氢氧化钠1-5g，氯化钠0.02-2g，三乙醇胺10-50mL，氢氧化钾1-5g，曲拉通1-10g，纯净水或蒸馏水120-180mL；
所述钙试纸的试剂配方为：偶氮胂I 0.1-1g，8羟基喹啉0.5-2g，硼酸钠0.2-5g，氢氧化钠1-8g，曲拉通1-10g，聚乙二醇1-5g，纯净水或蒸馏水200mL；
所述亚硝酸盐试纸的试剂配方为：0.2-3g盐酸萘乙二胺，10-50mL乙酸，对氨基苯磺酸0.5-4g，乙醇50-100mL，纯净水或蒸馏水50-150mL；
所述淀粉试纸的试剂配方为：1-4g碘化钾，0.2-2g碘，曲拉通1-5g，纯净水或蒸馏水200mL；
所述过氧化氢试纸的试剂配方为：四甲基联苯胺0.5-2g，辣根过氧化物酶0.002-0.02g，柠檬酸20-40g，柠檬酸钠15-40g，乙醇10-50mL，纯净水或蒸馏水60-150mL；
第二步：将大张的滤纸或者吸水纸等体积的浸入各种检测试纸的试剂中，全部浸透3-10分钟后立即送入30-80℃的烘箱中将其烘干；
第三步：将各种检测项目的试纸先剪切成条状，宽度控制在0.4-1厘米之间，有间隔地粘贴于底板上，再进行剪切，使每种检测试纸的尺寸控制在(0.4-1)厘米×(0.4-1)厘米之间，即得乳品多参数快速检测卡。
2.如权利要求1所述的乳品多参数快速检测卡的制备方法，其特征在于，所述过氧化氢试纸和亚硝酸盐试纸需低温避光保存。
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| CN110672598B (zh) * | 2019-10-24 | 2022-04-12 | 山东瑞思康生物科技有限公司 | 一种用于检测母乳钙、镁、锌的干化学试剂条 |
| CN115629064A (zh) * | 2022-11-30 | 2023-01-20 | 神州医疗科技股份有限公司 | 一种采样检测一体化装置及其控制方法 |
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