CN104316681A - Treponema pallidum specific antibody chemical light emitting detection kit and preparation method thereof - Google Patents
Treponema pallidum specific antibody chemical light emitting detection kit and preparation method thereof Download PDFInfo
- Publication number
- CN104316681A CN104316681A CN201410628360.6A CN201410628360A CN104316681A CN 104316681 A CN104316681 A CN 104316681A CN 201410628360 A CN201410628360 A CN 201410628360A CN 104316681 A CN104316681 A CN 104316681A
- Authority
- CN
- China
- Prior art keywords
- bottle
- specific antibody
- recombinant antigen
- treponema pallidum
- pallidum specific
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/571—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses for venereal disease, e.g. syphilis, gonorrhoea
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
Abstract
The invention discloses a treponema pallidum specific antibody chemical light emitting detection kit and a preparation method thereof, and relates to treponema pallidum. The kit comprises an outer package box, an alkaline phosphatase marked recombinant antigen bottle, a light emitting substrate bottle, a treponema pallidum specific antibody negative control bottle, a treponema pallidum specific antibody positive control bottle, a washing liquid bottle and a recombinant antigen coated micropore plate. The preparation method comprises the following steps: firstly, preparing reponema pallidum specific recombinant antigen and the recombinant antigen coated micropore plate, marking alkaline phosphatase of the recombinant antigen, further preparing a light emitting substrate, a washing liquid and a control group, and finally assembling the treponema pallidum specific antibody chemical light emitting detection kit. The treponema pallidum specific antibody chemical light emitting detection kit can be used for detecting syphilis specificity specific antibodies in clinical specimens, specific solid phase is adopted, immunity and light emitting reaction can be rapidly completed within a short time, the light emitting signals are greatly intensified, the sensitivity is improved, the detection time can be shortened, and the precision is improved.
Description
Technical field
The present invention relates to microspironema pallidum, especially relate to and adopt micro reaction plate chemiluminescence method Treponema pallidum specific antibody chemiluminescence detection kit carrying out Treponema pallidum specific antibody detection and preparation method thereof.
Background technology
Syphilis is the sexually transmitted disease caused by microspironema pallidum, and the incidence of disease at home rises year by year in recent years, and the prevention and control of syphilis have been classified as one of main task of China's public health service.
Microspironema pallidum can not in vitro culture, and directly aetology dark-field microscopy positive rate is not high, and Serologic test comprises cardiolipin antibody and detection of specific antibody, and the false positive rate of cardiolipin antibody is high, and sensitivity is low.And syphilis specific antibody detection method comprises the methods such as infant with congenital syphilis (TPPA), fluorescent treponemal antibody absorbed test (FTA-ABS) and Western blot (Western-blot) detects, its specificity is all higher.Chemiluminescence immune assay is the high degree of specificity, the high-affinity that utilize antigen-antibody reaction, and a kind of supersensitivity micro-quantitative technology that enzyme-catalyzed chemical luminescence reacts high-level efficiency and sets up, greatly can improve the convenience of operation, current responsive, the most special antibody mediated immunity quantitative measuring method can be referred to as, there is applications well prospect, current most chemiluminescence method adopts magnetic bead as reaction solid phase, and repeatability of its reaction affects larger by the size of magnetic bead particles and uniform degree.
Chinese patent CN101881772A discloses a kind of microspironema pallidum based on flow microsphere carrier technique (TP) antibody test kit and preparation and determination methods method thereof, specifically comprise with TP recombinant antigen bag by high dimeric molecule microballoon, close blank binding site with bovine serum albumin(BSA), make specificity T P probe-Gao dimeric molecule microballoon; Catch TP antibody with sample Dual culture to be measured, wash the unconjugated TP antibody of centrifugal removing, then add fluorescently-labeled anti-human igg or IgM antibody; Use the fluorescence intensity of flow cytomery microballoon, qualitative or quantitative test is carried out to tested antibodies.
Summary of the invention
The object of the present invention is to provide and adopt micro reaction plate chemiluminescence method Treponema pallidum specific antibody chemiluminescence detection kit carrying out Treponema pallidum specific antibody detection and preparation method thereof.The technological means of the detection of high sensitivity, high precision is provided for syphilis specific antibody test.
Described Treponema pallidum specific antibody chemiluminescence detection kit is provided with external packing box, alkali phosphatase enzyme mark recombinant antigen bottle, luminous substrate bottle, Treponema pallidum specific antibody negative controls bottle, microspironema pallidum specific positive control product bottle, Washing liquid bottle and recombinant antigen bag by microwell plate; Alkali phosphatase enzyme mark recombinant antigen bottle, luminous substrate bottle, Treponema pallidum specific antibody negative controls bottle, microspironema pallidum specific positive control product bottle, Washing liquid bottle and recombinant antigen bag are located in external packing box by microwell plate; Alkali phosphatase enzyme mark recombinant antigen bottle built with alkali phosphatase enzyme mark recombinant antigen, luminous substrate bottle built with luminous substrate, Treponema pallidum specific antibody negative controls bottle built with Treponema pallidum specific antibody negative controls, microspironema pallidum specific positive control product bottle built with microspironema pallidum specific positive control product, Washing liquid bottle built with cleansing solution.
The preparation method of described Treponema pallidum specific antibody chemiluminescence detection kit, comprises the following steps:
1) microspironema pallidum specificity recombinant antigen is prepared:
Adopt gene clone technology, the DNA of pcr amplification coding treponemal antigen, and insert in Escherichia coli and make it express, obtain microspironema pallidum specific antigen TPN17 and TPN47;
2) recombinant antigen bag is prepared by microwell plate
By step 1) in treponemal recombinant antigen TPN17 and TPN47 bag be buffered liquid and be diluted to 20 μ g/mL, add in microwell plate with every hole 0.1mL, 4 DEG C of bags are by 24h; Take out antigen plate, air-dry; With 1.0% skimmed milk power of 0.01mmol/L pH7.4 phosphate buffered saline, every hole 0.2mL, 4 DEG C of closed 24h, taking-up phosphate buffer washs 5 times, and room temperature is air-dry, sterilization, prepares recombinant antigen bag by microwell plate, seals for subsequent use;
3) alkali phosphatase enzyme mark of recombinant antigen
Alkaline phosphatase activates through sodium periodate, respectively with step 1)-NH2 the coupling of recombinant antigen molecule, form alkali phosphatase enzyme mark recombinant antigen;
4) luminous substrate preparation
Prof. Du Yucang diamantane amine luminous substrate (AMPPD) and reinforcing agent thereof, all through aseptic filtration, preparation luminous substrate working fluid;
5) cleansing solution preparation
Cleansing solution is the phosphate buffer being dissolved with Tween-20, and wherein the final concentration of Tween-20 is 0.01%;
6) reference substance
Treponema pallidum specific antibody negative controls: formulated by the healthy population serum of non-syphilis;
Microspironema pallidum specific positive control product: the specific antibody positive serum of syphilitic is formulated;
7) Treponema pallidum specific antibody chemiluminescence detection kit is prepared
Alkali phosphatase enzyme mark recombinant antigen, luminous substrate, cleansing solution, Treponema pallidum specific antibody negative controls, Treponema pallidum specific antibody positive reference substance are respectively charged in bottle, again alkali phosphatase enzyme mark recombinant antigen bottle, luminous substrate bottle, Treponema pallidum specific antibody negative controls bottle, microspironema pallidum specific positive control product bottle, Washing liquid bottle and recombinant antigen bag are loaded external packing box by microwell plate, composition Treponema pallidum specific antibody chemiluminescence detection kit.
In step 7) in, described bottle can adopt polyethylene bottle.
The invention provides a kind of microspironema pallidum Treponema pallidum specific antibody chemiluminescence detection kit, can be used for the detection of syphilis specific specific antibody in clinical samples, adopt special solid phase, immunity and luminescence-producing reaction are completed in the short period of time fast, luminous signal strengthens greatly, sensitivity is improved, and detection time can shorten thus, and accuracy is improved.
The present invention adopts micro reaction plate chemoluminescence method to carry out the detection of Treponema pallidum specific antibody, adopt special solid phase, immunity and luminescence-producing reaction are completed in the short period of time fast, luminous signal strengthens greatly, sensitivity is improved, detection time can shorten thus, and accuracy is improved.
Accompanying drawing explanation
Fig. 1 is the structure composition schematic diagram of Treponema pallidum specific antibody chemiluminescence detection kit embodiment of the present invention.
Embodiment
Following examples will the present invention is further illustrated by reference to the accompanying drawings.
Embodiment 1
See Fig. 1, described Treponema pallidum specific antibody chemiluminescence detection kit is provided with external packing box 1, alkali phosphatase enzyme mark recombinant antigen bottle 2, luminous substrate bottle 3, Treponema pallidum specific antibody negative controls bottle 4, microspironema pallidum specific positive control product bottle 5, Washing liquid bottle 6 and recombinant antigen bag by microwell plate 7; Alkali phosphatase enzyme mark recombinant antigen bottle 2, luminous substrate bottle 3, Treponema pallidum specific antibody negative controls bottle 4, microspironema pallidum specific positive control product bottle 5, Washing liquid bottle 6 and recombinant antigen bag are located in external packing box 1 by microwell plate 7; Alkali phosphatase enzyme mark recombinant antigen bottle 2 built with alkali phosphatase enzyme mark recombinant antigen, luminous substrate bottle 3 built with luminous substrate, Treponema pallidum specific antibody negative controls bottle 4 built with Treponema pallidum specific antibody negative controls, microspironema pallidum specific positive control product bottle 5 built with microspironema pallidum specific positive control product, Washing liquid bottle 6 built with cleansing solution.
Described Treponema pallidum specific antibody chemiluminescence detection kit and preparation thereof, comprise the following steps:
(1) microspironema pallidum specificity recombinant antigen is prepared:
Adopt gene clone technology, the DNA of pcr amplification coding treponemal antigen, and insert in Escherichia coli and make it express, obtain microspironema pallidum specific antigen TPN17 and TPN47.
(2) recombinant antigen bag is prepared by microwell plate
Treponemal recombinant antigen TPN17 in step (1) and TPN47 bag are buffered liquid and are diluted to 20 μ g/mL, add in microwell plate with every hole 0.1mL, 4 DEG C of bags are by 24h; Take out antigen plate, air-dry; With 1.0% skimmed milk power of 0.01mmol/L pH7.4 phosphate buffered saline, every hole 0.2mL, 4 DEG C of closed 24h, taking-up phosphate buffer washs 5 times, and room temperature is air-dry, sterilization, prepares recombinant antigen bag by microwell plate, seals for subsequent use.
(3) alkali phosphatase enzyme mark of recombinant antigen
Alkaline phosphatase activates through sodium periodate, respectively with the-NH2 coupling of step (1) recombinant antigen molecule, forms alkali phosphatase enzyme mark recombinant antigen.
(4) luminous substrate preparation
Prof. Du Yucang diamantane amine luminous substrate (AMPPD) and reinforcing agent thereof, all through aseptic filtration, preparation luminous substrate working fluid.
(5) cleansing solution preparation
Cleansing solution is the phosphate buffer being dissolved with Tween-20, and wherein the final concentration of Tween-20 is 0.01%.
(6) reference substance
Treponema pallidum specific antibody negative controls: formulated by the healthy population serum of non-syphilis;
Microspironema pallidum specific positive control product: the specific antibody positive serum of syphilitic is formulated;
(7) syphilis helicoid antibody high flux detection kit is prepared
The Treponema pallidum specific antibody chemiluminescence detection kit that recombinant antigen bag is formed jointly by microwell plate, alkali phosphatase enzyme mark recombinant antigen, luminous substrate, cleansing solution, Treponema pallidum specific antibody negative controls, Treponema pallidum specific antibody positive reference substance and external packing box.
Step (7) described alkali phosphatase enzyme mark recombinant antigen, luminous substrate, cleansing solution, Treponema pallidum specific antibody negative controls, Treponema pallidum specific antibody positive reference substance are all contained in corresponding polyethylene bottle.
Embodiment 2
Below provide the Treponema pallidum specific antibody in the clinical samples adopting syphilis helicoid antibody high flux detection kit detection patient:
1, sample disposal: serum: venous blood 5mL, puts 37 DEG C of water-bath 30min, the centrifugal 10min of 3000g, and supernatant is for subsequent use for detecting sample.
2, application of sample: the sample adding 100 μ L, in reaction plate, makes blank, feminine gender and Positive control wells simultaneously.Hatch 1h for 37 DEG C.
3, wash: after 37 DEG C of reaction 30min, the syphilis specific recombinant antigen that determined syphilis specific antibody wraps quilt on microwell plate is combined, and washing is separated unconjugated free composition;
4, add alkali phosphatase enzyme mark recombinant antigen, then add luminous substrate working fluid, alkaline phosphatase substrate for enzymatic activity dephosphorylation base, and send the visible ray of 463nm.The relative luminous intensity unit (relative light units, RLU) respectively adding sample well is measured in 10-20min.The RLU of sample is relevant to syphilis antibody concentration positive to be measured.
Embodiment 3
Below provide the performance detecting of syphilis helicoid antibody high flux detection kit.
(1) positive sample coincidence rate
With syphilis specific antibody positive control serum 50 parts calibrating, calculate positive coincidence rate.
(2) ' negative ' specimens coincidence rate
With syphilis specific negative antibody control serum 50 parts calibrating, calculate negative match-rate.
(3) interior difference is criticized
Same batch of kit, uses characteristic Virus monitory, requires CV≤10%.
(4) differences between batches
Different batches kit, uses characteristic Virus monitory, requires CV≤12%.
(5) interference test
The interference experiment undertaken by each 50 examples of haemolysis, piarhemia and jaundice sample detects.
(6) cross reaction
Adopt this kit, carry out the detection of the autoimmune pathologies such as systemic loupus erythematosus (n=50), rheumatoid disease (n=50), autoallergic (n=50), observe cross reaction.
(7) Detection of Stability
Application Arrhenius rule, kit is placed 37 DEG C after 20 days and detect, above indices, without marked change, guarantees that finished product is preserved under drying at room temperature condition, and the term of validity is 18 months.
Claims (3)
1. Treponema pallidum specific antibody chemiluminescence detection kit, is characterized in that being provided with external packing box, alkali phosphatase enzyme mark recombinant antigen bottle, luminous substrate bottle, Treponema pallidum specific antibody negative controls bottle, microspironema pallidum specific positive control product bottle, Washing liquid bottle and recombinant antigen bag by microwell plate; Alkali phosphatase enzyme mark recombinant antigen bottle, luminous substrate bottle, Treponema pallidum specific antibody negative controls bottle, microspironema pallidum specific positive control product bottle, Washing liquid bottle and recombinant antigen bag are located in external packing box by microwell plate; Alkali phosphatase enzyme mark recombinant antigen bottle built with alkali phosphatase enzyme mark recombinant antigen, luminous substrate bottle built with luminous substrate, Treponema pallidum specific antibody negative controls bottle built with Treponema pallidum specific antibody negative controls, microspironema pallidum specific positive control product bottle built with microspironema pallidum specific positive control product, Washing liquid bottle built with cleansing solution.
2. the preparation method of Treponema pallidum specific antibody chemiluminescence detection kit as claimed in claim 1, is characterized in that comprising the following steps:
1) microspironema pallidum specificity recombinant antigen is prepared:
Adopt gene clone technology, the DNA of pcr amplification coding treponemal antigen, and insert in Escherichia coli and make it express, obtain microspironema pallidum specific antigen TPN17 and TPN47;
2) recombinant antigen bag is prepared by microwell plate
By step 1) in treponemal recombinant antigen TPN17 and TPN47 bag be buffered liquid and be diluted to 20 μ g/mL, add in microwell plate with every hole 0.1mL, 4 DEG C of bags are by 24h; Take out antigen plate, air-dry; With 1.0% skimmed milk power of 0.01mmol/L pH7.4 phosphate buffered saline, every hole 0.2mL, 4 DEG C of closed 24h, taking-up phosphate buffer washs 5 times, and room temperature is air-dry, sterilization, prepares recombinant antigen bag by microwell plate, seals for subsequent use;
3) alkali phosphatase enzyme mark of recombinant antigen
Alkaline phosphatase activates through sodium periodate, respectively with step 1)-NH2 the coupling of recombinant antigen molecule, form alkali phosphatase enzyme mark recombinant antigen;
4) luminous substrate preparation
Prof. Du Yucang diamantane amine luminous substrate and reinforcing agent thereof, all through aseptic filtration, preparation luminous substrate working fluid;
5) cleansing solution preparation
Cleansing solution is the phosphate buffer being dissolved with Tween-20, and wherein the final concentration of Tween-20 is 0.01%;
6) reference substance
Treponema pallidum specific antibody negative controls: formulated by the healthy population serum of non-syphilis;
Microspironema pallidum specific positive control product: the specific antibody positive serum of syphilitic is formulated;
7) Treponema pallidum specific antibody chemiluminescence detection kit is prepared
Alkali phosphatase enzyme mark recombinant antigen, luminous substrate, cleansing solution, Treponema pallidum specific antibody negative controls, Treponema pallidum specific antibody positive reference substance are respectively charged in bottle, again alkali phosphatase enzyme mark recombinant antigen bottle, luminous substrate bottle, Treponema pallidum specific antibody negative controls bottle, microspironema pallidum specific positive control product bottle, Washing liquid bottle and recombinant antigen bag are loaded external packing box by microwell plate, composition Treponema pallidum specific antibody chemiluminescence detection kit.
3. the preparation method of Treponema pallidum specific antibody chemiluminescence detection kit as claimed in claim 1, is characterized in that in step 7) in, described bottle adopts polyethylene bottle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410628360.6A CN104316681A (en) | 2014-11-10 | 2014-11-10 | Treponema pallidum specific antibody chemical light emitting detection kit and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410628360.6A CN104316681A (en) | 2014-11-10 | 2014-11-10 | Treponema pallidum specific antibody chemical light emitting detection kit and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104316681A true CN104316681A (en) | 2015-01-28 |
Family
ID=52371939
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410628360.6A Pending CN104316681A (en) | 2014-11-10 | 2014-11-10 | Treponema pallidum specific antibody chemical light emitting detection kit and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104316681A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1405565A (en) * | 2002-10-24 | 2003-03-26 | 肖洪武 | Diagnosis reagent for syphilis spirochete antibody |
| WO2007002178A2 (en) * | 2005-06-21 | 2007-01-04 | The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Methods, immunoassays and devices for detection of anti-lipoidal antibodies |
| CN101363860A (en) * | 2007-08-06 | 2009-02-11 | 北京科美东雅生物技术有限公司 | Syphilis helicoid antibody chemiluminescence immune assay determination kit and method for preparing same |
| CN101738473A (en) * | 2008-11-13 | 2010-06-16 | 威海威高生物科技有限公司 | Treponema pallidum antibody diagnostic kit and preparation method thereof |
-
2014
- 2014-11-10 CN CN201410628360.6A patent/CN104316681A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1405565A (en) * | 2002-10-24 | 2003-03-26 | 肖洪武 | Diagnosis reagent for syphilis spirochete antibody |
| WO2007002178A2 (en) * | 2005-06-21 | 2007-01-04 | The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Methods, immunoassays and devices for detection of anti-lipoidal antibodies |
| CN101363860A (en) * | 2007-08-06 | 2009-02-11 | 北京科美东雅生物技术有限公司 | Syphilis helicoid antibody chemiluminescence immune assay determination kit and method for preparing same |
| CN101738473A (en) * | 2008-11-13 | 2010-06-16 | 威海威高生物科技有限公司 | Treponema pallidum antibody diagnostic kit and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP6553020B2 (en) | Automated immunoassay system for performing diagnostic analysis on allergies and autoimmune diseases | |
| CN103364568B (en) | Laminin nano-magnetic particle chemiluminescent immunity quantitative detection kit and preparation method thereof | |
| CN101363860A (en) | Syphilis helicoid antibody chemiluminescence immune assay determination kit and method for preparing same | |
| CN101533011A (en) | A Japanese blood fluke ovum antibody magnetic particle EILSA detecting method | |
| CN101178404B (en) | Human immunodeficiency virus antibody chemiluminescence immune analyzing diagnose reagent box and method of producing the same | |
| Gao et al. | A rapid assay for Hendra virus IgG antibody detection and its titre estimation using magnetic nanoparticles and phycoerythrin | |
| CN106324254A (en) | Anti-insulin antibody detection kit and detection method thereof | |
| CN104330562A (en) | High-throughput treponema pallidum specific antibody detection kit and preparation method thereof | |
| CN104155450A (en) | Treponema pallidum IgG antibody biotin avidin enzyme-linked immune detection kit and preparation method thereof | |
| CN109342718A (en) | A kind of magnetic microparticle chemiluminescence detection method | |
| JPH03502248A (en) | Aqueous washing solution for assay, diagnostic test kit and method for measuring herpes simplex virus | |
| CN106405098A (en) | An anti-beta2 glycoprotein I antibody chemiluminescence immunodetection kit and a preparing method thereof | |
| CN107966563A (en) | A kind of antimyeloperoxidase antibody IgG chemiluminescence immunoassay kits and preparation method thereof | |
| CN101551395A (en) | Chemiluminescence immunoassay kit of hepatitis E virus IgM antibody and preparation method thereof | |
| CN106248943A (en) | Antinuclear antibody chemiluminescence immune detection reagent kit and preparation method thereof | |
| CN104316681A (en) | Treponema pallidum specific antibody chemical light emitting detection kit and preparation method thereof | |
| CN109884305B (en) | Homogeneous phase chemiluminescence micro-fluidic chip and detection method thereof | |
| CN202975014U (en) | Multi-index detection device and kit | |
| CN106198958A (en) | Antisperm antibody chemiluminescence immune detection reagent kit and preparation method thereof | |
| CN101533026A (en) | Chemoluminescent immunoassay kit of hepatitis A virus IgG antibody and preparation method thereof | |
| CN104237501A (en) | Antibody-modified nanometer silver, reagent box and application thereof | |
| CN104360067B (en) | Treponema pallidum specific antibody immue quantitative detection reagent box and preparation method thereof | |
| CN106053836A (en) | Endometrial antibody chemiluminescence immunoassay kit and preparation method thereof | |
| CN107894505A (en) | A kind of method of quick detection beta-lactam antibiotics residue | |
| CN101592663A (en) | Hepatitis B e antigen magnetic particle chemiluminescent immunoassay kit and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150128 |