CN101533026A - Chemoluminescent immunoassay kit of hepatitis A virus IgG antibody and preparation method thereof - Google Patents
Chemoluminescent immunoassay kit of hepatitis A virus IgG antibody and preparation method thereof Download PDFInfo
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- CN101533026A CN101533026A CN200810102002A CN200810102002A CN101533026A CN 101533026 A CN101533026 A CN 101533026A CN 200810102002 A CN200810102002 A CN 200810102002A CN 200810102002 A CN200810102002 A CN 200810102002A CN 101533026 A CN101533026 A CN 101533026A
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Abstract
The invention relates to the field of immunoassay medical science, and in particular provides a chemoluminescent immunoassay kit of a hepatitis A virus IgG antibody and a preparation method thereof. The kit comprises: 1) negative and positive control varieties of the hepatitis A virus IgG antibody; 2) a solid phase carrier dermatated by a hepatitis A virus antigen; 3) an enzyme labeling combiner; 4) a chemoluminescent substrate; and 5) a condensed washing solution. Furthermore, the method for preparing the kit comprises the following steps: 1) preparing control varieties from negative and positive serum of the hepatitis A virus IgG antibody; 2) dermatating the solid phase carrier by the hepatitis A virus antigen; 3) labeling the hepatitis A virus antibody (polyclonal antibody or monoclonal antibody) by enzyme; 4) preparing the luminescent substrate solution; 5) preparing the condensed washing solution; 6) sub-packaging the negative and positive control varieties of the hepatitis A virus IgG antibody, the enzyme labeling combiner, the chemoluminescent substrate and the condensed washing solution; and 7) assembling the substances into the finished products. The kit has the advantages of simpleness, convenience, quickness, sensitiveness, stability, and the like.
Description
Technical field
The present invention relates to the immunoassay medical domain, particularly, the invention provides a kind of Chemoluminescent immunoassay kit of hepatitis A virus IgG antibody and preparation method thereof.
Background technology
Hepatitis A is to propagate a kind of communicable disease based on liver damage that causes by fecal oral route by hepatitis A virus (HAV).Be a kind of RNA viruses, belong to pico+ribonucleic acid+virus section, be enterovirus 72 types, the about 27nm of diameter spherical in shape forms 20 body nucleocapsids of symmetry by 32 shell particulates, includes the line style single-stranded RNA.HAV has 4 main polypeptide, for constituting the major antigen polypeptide of virus coat protein, induces neutralizing antibody.HAV is present in patient's blood, ight soil and the liver endochylema.Infect anti--very fast appearance of HAVIgM antibody in the serum of back, about 2 weeks, reach the peak.Descending gradually then, disappear within 8 weeks, is the serological evidence of HAV recent infection; Anti--HAVIgG antibody produces later, occurs (when IgM began to descend, IgG rose gradually) in later stage acute stage and convalescence in early days and belongs to protection antibody, reaches the peak in convalescence, can exist lastingly, can protect no longer subinfection of human body; Significant to hepatitis A epidemiology survey, health examination, understanding Susceptible population.
Hepatitis A virus (HAV) IgG antibody laboratory detection method is mainly immunoassay, and detection method commonly used is that antibody is surveyed in radiommunoassay (RIA) and EIA enzyme immunoassay (EIA), colloidal gold immunochromatographimethod, spot immune dialysis, hemagglutination-inhibition test.Use at present the widest be EIA enzyme immunoassay (EIA).
Along with the research and the application development of labelled immune analytical technology are rapid, be widely used in each field of biomedical fundamental research and clinical disease diagnosis.Wherein the technical matters maturation has advance and practical, and what be easy to promote mainly contains: four kinds of radiommunoassay, EIA enzyme immunoassay, time resolved fluoro-immunoassay and chemiluminescence immune assays etc.The basic theories of these ultramicron detection techniques is identical substantially, but used tracer agent and the signal that sent have nothing in common with each other.According to a large amount of test findings and clinical practice data,, be followed successively by: chemiluminescence immune assay, time resolved fluoro-immunoassay, radiommunoassay and EIA enzyme immunoassay from practicality, stability, accuracy and development prospect.Chemiluminescence immunoassay technology, both had the high sensitivity of not failing in methods such as radiommunoassay, time resolved fluoro-immunoassays, overcome shortcomings such as the short and time resolved fluoro-immunoassay poor stability of the radioactive contamination, half life period of radiommunoassay, instrument costliness again, can be widely used in biomedical research and clinical medical inspection.More can be fit to China's actual conditions, be convenient to apply in large, medium and small hospital.
At present, chemiluminescence immunoassay technology is not seen yet in the application facet of hepatitis A virus IgG antibody immunoassay product.Kit of the present invention adopts the microwell plate chemiluminescence immunoassay technology, be on the basis of enzyme-linked immuno assay, to use the enzymatic luminous substrate, by the chromogenic substrate in the light signal replacement enzyme-linked immuno assay that detects the luminous substrate generation, thereby it is highly sensitive; Specificity is good, reduces the judgement of gray area sample, has reduced the probability that false positive and negative result occurs.Both can be applicable to the open luminous measuring instrument of semi-automatic chemistry, and also can be used for full automatic measuring system, and can realize fast detection the in enormous quantities, use cost is low, easier applying.
Summary of the invention
The present invention has solved the problems referred to above well, soon chemiluminescence is learned effectively with the immunoassay of hepatitis A virus IgG antibody and is combined, a kind of kit that can easy, quick, sensitive, stably detect hepatitis A virus IgG antibody is provided, and this kit is suitable for applying effectively on industry.
The purpose of this invention is to provide a kind of hepatitis A virus IgG antibody chemical luminescence immune assay determination reagent kit and preparation method thereof.
Kit according to the present invention comprises: 1) hepatitis A virus IgG antibody positive and negative reference substance; 2) solid phase carrier of hepatitis A viral antigen bag quilt; 3) enzyme labeling bond; 4) chemical luminous substrate that above-mentioned enzyme acted on; And 5) concentrated cleaning solution.
According to kit of the present invention, wherein, described bag is microwell plate, plastic bead, plastic tube or magnetic-particle by solid phase carrier;
According to kit of the present invention, wherein, the hepatitis A virus antibody that described enzyme labeling bond is alkaline phosphatase or horseradish peroxidase-labeled (resisting or monoclonal antibody) more;
According to kit of the present invention, wherein, described chemical luminous substrate is 1,2-two oxidative ethane analog derivatives, luminol or different luminol, wherein said 1,2-two oxidative ethane analog derivatives are (diamantane)-1,2-two oxidative ethanes, 3-(2 '-the spiral diamantane)-4-methoxyl-4-(3 " the phosphorus acyloxy) phenyl-1,2-two oxidative ethanes (AMPPD), CSPD or CDP-Star.
Further, the invention provides a kind of method for preparing the mentioned reagent box, may further comprise the steps:
1) with hepatitis A virus IgG antibody positive and negative serum preparation reference substance;
2) with hepatitis A viral antigen direct coated carrier;
3) with enzyme labeling anti-HAV (resisting or monoclonal antibody) more;
4) preparation luminous substrate liquid
5) preparation concentrated cleaning solution
6) chemical luminous substrate that acted on of the above-mentioned hepatitis A virus IgG of packing antibody positive and negative reference substance, enzyme labeling bond and enzyme; And
7) be assembled into finished product.
The method according to this invention, preferred, described bag is by the step 2 of solid phase carrier) may further comprise the steps:
1) bag quilt
With 0.1M pH value is the hepatitis A viral antigen coating buffer that 9.0 dipotassium hydrogen phosphate damping fluid is mixed with desired concn, and coating buffer is carried on the solid phase carrier;
2) sealing
With containing 2% BSA, 0.015% gelatin, the pH value is 6.8～7.3, concentration is that the phosphate buffer of 0.01M is as the above-mentioned solid phase carrier of confining liquid sealing.
In said method, preferred, described antigen coated solid phase carrier is microwell plate, plastic bead, plastic tube or magnetic-particle; Described bond is horseradish peroxidase, alkaline phosphatase; Described chemical luminous substrate is 1,2-two oxidative ethane analog derivatives, luminol or different luminol, wherein said 1,2-two oxidative ethane analog derivatives are (diamantane)-1,2-two oxidative ethanes, 3-(2 '-the spiral diamantane)-4-methoxyl-4-(3 " the phosphorus acyloxy) phenyl-1,2-two oxidative ethanes, CSPD or CDP-Star.
Concrete mentioned reagent box can comprise hepatitis A virus IgG antibody positive and negative reference substance, direct coated hepatitis A viral antigen microwell plate, enzyme labeling bond and chemical luminous substrate liquid, concentrated cleaning solution etc.Wherein, described hepatitis A virus IgG antibody positive and negative reference substance is through HBsAg, anti-HIV, anti--TP and many parts of all negative pooled serums of anti-HCV TPPA, 60 ℃ of deactivations 1 hour, appropriateness dilution preparation (anti--HAVIgG antibody positive is tired〉1:1000); The microwell plate of direct coated is the micropore lath in 48 or 96 holes, and the enzyme of labelled antibody is coupling horseradish peroxidase (HRP), and chemical luminous substrate liquid is luminol, and concentrated cleaning solution is PBST.
The present invention's " hepatitis A virus IgG antibody chemical luminescence immune assay determination reagent kit " can detect very single-mindedly and infect the hepatitis A virus IgG antibody in the human body behind the hepatitis A virus, can judge the variation of the result of treatment and the state of an illness thereof according to hepatitis A virus IgG antibody situation of change.It has advantages such as easy, quick, sensitive, stable.And according to detection system of the present invention is open-sky technique, does not easyly fast need the expensive luminous measuring instrument of full-automatic chemical, more can be fit to vast middle and small hospital and promote the use of, for clinical diagnosis and research work provide a kind of very valuable detection means.According to kit of the present invention, the hepatitis A virus IgG antibody of the antibody of enzyme labeling and sample combines with the antigenic competition on the carrier, therefore " competition combined techniques " reaction pattern of the present invention's employing had both effectively utilized the chemiluminescence principle, had guaranteed the sensitivity that detects again.In addition, this pattern also is convenient to operation and is produced.
What kit of the present invention was used is the enzymatic luminous substrate, by the chromogenic substrate in the light signal replacement EIA enzyme immunoassay that detects the luminous substrate generation, thereby have a specificity equal with EIA enzyme immunoassay, and the sensitivity of highly sensitive EIA enzyme immunoassay in now, the diagnosis that can be hepatitis A virus IgG antibody provides more special, quick, reliable foundation.
Embodiment
Embodiment 1 preparation hepatitis A virus IgG antibody chemical luminescence immune assay determination reagent kit of the present invention
In research process of the present invention, the present inventor has at first carried out screening experiment and Quality Identification to used starting material, comprises the luminous intensity of activity, chemical luminous substrate of the absorption property of activity, carrier (as lighttight white microwell plate) of labelled antibody and coated antibody and variation size, HRP and luminous duration etc.Then method for coating is studied, be cushioned liquid and protect liquid to experimentize, select optimal bag and be cushioned liquid and protection liquid, tested by concentration by the different bags of antigen and find best concentration conditions with different bags.Mark for HRP can have diverse ways, by explore repeatedly and the contrast experiment finally found easy, productive rate is high, cost is low, the reliable quality labeling method, and different enzyme dilutions has been carried out long-term investigation tested, made and can make the activity stabilized dilution of the long-term maintenance of enzyme labeling thing.
One, enzyme labelled antibody preparation
The antibody of hepatitis A virus (ELIAS tires〉1:4000) with glutaraldehyde method and horseradish peroxidase, fully dialyse with PBS, add equal-volume glycerine, preserve below-20 ℃.
Two, the preparation of hepatitis A virus IgG antibody positive and negative reference substance
Mix more than 6 parts and detect hepatitis A virus IgG antibody positive serum or negative serum through the ELISA kit, be negative serum through HBsAg, anti-HIV, anti--TP and anti-HCV TPPA, 60 ℃ of deactivations 1 hour, filtration sterilization, appropriateness dilution, packing, low temperature is frozen.
Three, enzyme labelled antibody concentration is selected
Enzyme labelled antibody dilution: be borax 1.4304g, boric acid 5.2564g, calf serum 200ml, glycerine 20ml, Proclin300 1ml, distilled water dissolving, fixed molten 1000ml.
Adopt the square formation method to select the working concentration of enzyme labelled antibody.1:6000
Four, the preparation of solid-phase coating plate
(1) bag quilt
The dipotassium hydrogen phosphate distilled water that takes by weighing 22.823g is separated molten surely 1000ml, adjusts PH to 9.0, adds an amount of hepatitis A viral antigen mixing, add then in each hole of microwell plate, and every hole 100 μ L, 4 ℃ are spent the night.
(2) sealing
Take by weighing 0.6944g NaH
2PO
42H
2O, 2.1851g NaH
2PO
412H
2O, 20g BSA, 0.15g gelatin, 8.5gNaCL put into clean container, and add and add the fixed molten 1000ml of Proclin300 1ml after distilled water dissolves,, measuring the pH value is 6.8～7.3.
Every hole adds confining liquid 150 μ L respectively, adsorbs 24 ℃ hours.Get rid of confining liquid, on thieving paper, pat dry.Room temperature removal moisture drying 24 hours.Carry out vacuum sealing bag immediately.Place behind the envelope and checked not have gas leakage in 15 minutes, if there is gas leakage to need envelope again.2～8 ℃ of preservations of labeling postposition.
Five, chemical luminous substrate A liquid
Take by weighing 1.7716g luminol, 0.051g 4-xenol, 0.012g 4-iodobenzene boric acid, 11.4g boric acid, the dissolving of 4.9g borax distilled water, fixed molten 1000ml surveys pH 8.0～10.0;
Six, chemical luminous substrate B liquid
Take by weighing 0.329g urea peroxide, 1ml Tween20,51.58g Na
2HPO412H
2O, 8.74g NaH
2PO
42H
2The dissolving of O distilled water, fixed molten 1000ml, surveying the pH value is 7.0～7.6.
A liquid mixes with B liquid equal proportion during use.
Seven, 20 times of concentrated cleaning solutions
Take by weighing 58g Na
2HPO
412H
2O, 2g NaH
2PO
42H
2O, 160g NaCl, 1mL Tween-20, the fixed molten 1000ml of 1mLProclin 300 distilled waters dissolving survey pH 7.2～7.4.
Eight, semi-manufacture and finished product are formed
The packing of above-mentioned steps products obtained therefrom is semi-manufacture.Extract three parts of process specificitys, accuracy, sensitivity and stable assay approvals out and just can be assembled into hepatitis A virus IgG TPPA kit (chemoluminescence method).Be assembled into also need inspect by random samples behind the kit and just can dispatch from the factory after qualified.
To sum up, the present inventor has also carried out experimental study to the reaction pattern and the reaction conditions of kit, has finally determined the competition law reaction pattern, and the influence of experimental result is tested with regard to the different reaction time, determines the optimal reaction time.By the influence experiment to measured value shows to the mensuration of luminous duration of chemical luminous substrate liquid and different fluorescent lifetime: be measured as the best between 5-25 minute after adding chemical luminous substrate liquid, its result is also comparatively accurate.
Embodiment 2～3 preparations hepatitis A virus IgG antibody chemical luminescence immune assay determination reagent kit of the present invention
Divided by the alkali phosphatase enzyme mark anti-HAV, with AMPPD as chemical luminous substrate, with plastic bead, plastic tube as the pH value of solid phase carrier, coating buffer be 4.5, the pH value of confining liquid is outside 7.5, all the other all prepare hepatitis A virus IgG antibody chemical luminescence immune assay determination reagent kit with the method identical with embodiment 1.
Embodiment 4 preparations hepatitis A virus IgG antibody chemical luminescence immune assay determination reagent kit of the present invention
As solid phase carrier, adopt the EDC coupling method to prepare outside the magnetic particulate antigen solid phase carrier divided by magnetic-particle, all the other all prepare hepatitis A virus IgG antibody chemical luminescence immune assay determination reagent kit with the method identical with embodiment 1.
The using method of embodiment 5 kits of the present invention
The concrete operations of the hepatitis A virus IgG antibody chemical luminescence immune assay determination reagent kit of above embodiment 1 preparation are as follows:
1) in 4 ℃ of refrigerators, takes out kit, equilibrium at room temperature 15 minutes.
2) take out coated slab, insert on the grillage.
3) except that blank well, add each two hole of yin, yang reference substance in the reacting hole respectively, every hole 50ul, each hole adds sample 50ul to be checked, enzyme-added then label 50 μ L, with the micro-oscillator mixing that fully vibrates, 37 ℃ of incubations 0.5 hour.
4) get rid of dereaction liquid, the cleansing solution after the dilution is filled it up with in every hole, washes plate 5 times, buckles on clean thieving paper at last and does.
5) each hole adds each 50 μ L of chemical luminous substrate liquid A, B, and fully vibrating with micro-oscillator mixes, room temperature (20～25 ℃) lucifuge reaction 5 minutes.
6) must measure in the 5th～25 minute after adding chemical luminous substrate liquid, on the chemiluminescence measuring instrument, measure the luminous intensity (RLU) in each hole in regular turn, 1 second/hole of Measuring Time.
7) Cutoff value=0.5N, RLU value 〉=Cutoff value then sample is judged to be negative sample; RLU value＜Cutoff value then sample is judged to be positive sample.
The methodology of embodiment 6 kits of the present invention is identified
According to manufacturing conventional in this area and vertification regulation the kit of preparation among the embodiment 1 is examined and determine, is seen the following form 1:
Table 1
Sensitivity, specificity, accuracy and stability that " hepatitis A virus IgG antibody (anti--HAVIgG) chemical luminescence method immune analysis is measured kit " is described meet the calibrating requirement fully.
Embodiment 7 hepatitis A virus IgG antibody of the present invention (anti--HAVIgG) clinical practice and the ELISA kit of chemical luminescence method immune analysis mensuration kit compare
Three tame clinical hospitals use embodiment 1 hepatitis A virus IgG antibody (anti--HAVIgG) chemical luminescence method immune analysis measure kit and ELISA kit (this kit for obtain State Food and Drug Administration produce the detection of official written reply anti--the ELISA product of HAVIgG) detect 1201 parts of clinical samples, to contrast the coincidence rate that two kinds of kits detect.
One, the source of clinical serum specimen
Hepatitis A virus that each hospital clinical is collected and non-hepatitis A virus patient and normal person's sample.
Two, use hepatitis A virus IgG antibody (anti--HAVIgG) chemical luminescence method immune analysis mensuration kit and the comparison of ELISA kit
1, method
Three tame hospitals use the embodiment of the invention 1 preparation " hepatitis A virus IgG antibody (anti--HAVIgG) chemical luminescence method immune analysis is measured kit " (by its instructions operation) and ELISA kit (by its instructions operation) to detect 1201 parts of clinical samples respectively, to contrast the coincidence rate of two kinds of kits detections.
2, result
The three tame use embodiment 1 of hospitals " hepatitis A virus IgG antibody (anti--HAVIgG) chemical luminescence method immune analysis is measured kit " and ELISA kit comparison and detection result (seeing Table 2)
Two kinds of methods of table 2. three tame hospital's contrasts relatively
The use of three tame hospitals " hepatitis A virus IgG antibody (anti--HAVIgG) chemical luminescence method immune analysis is measured kit " and showing with ELISA kit comparison and detection result: every index of kit of the present invention has has all met or exceeded the ELISA method, its specificity 100% is apparently higher than ELISA reagent; Susceptibility 100% and conform to ELISA method height has obviously been avoided the result's of gray area blood serum sample judgement, has reduced the appearance of the blood serum sample false positive results of surveying.
Hepatitis A virus IgG antibody of the present invention (anti--HAVIgG) chemical luminescence immune assay determination reagent kit, can be applied to clinical hepatitis A medical diagnosis on disease.
Claims (10)
1, a kind of hepatitis A virus IgG antibody chemical luminescence immune assay determination reagent kit is characterized in that described kit comprises:
1) hepatitis A virus IgG antibody positive and negative reference substance;
2) solid phase carrier of hepatitis A viral antigen bag quilt;
3) enzyme labeling bond;
4) chemical luminous substrate that above-mentioned enzyme acted on; And
5) concentrated cleaning solution.
2, kit as claimed in claim 1 is characterized in that, described solid phase carrier is that the solid phase carrier of hepatitis A viral antigen bag quilt is microwell plate, plastic bead, plastic tube or magnetic-particle.
3, kit as claimed in claim 1 is characterized in that, the hepatitis A virus antibody that described enzyme labeling bond is alkaline phosphatase or horseradish peroxidase-labeled (resisting or monoclonal antibody) more.
4, kit as claimed in claim 1 is characterized in that, described chemical luminous substrate is 1,2-two oxidative ethane analog derivatives, luminol or different luminol.
5, kit as claimed in claim 1 is characterized in that, described chemical luminous substrate comprises A liquid and B liquid, wherein,
Described chemical luminous substrate A liquid comprises 10mM luminol, 0.3mM 4-xenol, 0.05mM 4-iodine substituted phenyl boric acid, 0.2M pH8.7 boric acid-borate buffer solution, and its pH value is 8.0～10.0;
Described chemical luminous substrate B liquid comprises 3.5mM urea peroxide, 0.1%Tween20,0.2M pH7.2 phosphate buffer, and its pH value is 7.0～7.6.
6, a kind of method for preparing the described kit of claim 1 is characterized in that may further comprise the steps:
1) with hepatitis A virus IgG antibody positive and negative serum preparation reference substance;
2) with the hepatitis A viral antigen bag by solid phase carrier;
3) with enzyme labeling anti-HAV (resisting or monoclonal antibody) more;
4) preparation luminous substrate liquid;
5) preparation concentrated cleaning solution;
6) chemical luminous substrate and the concentrated cleaning solution that are acted on of the above-mentioned hepatitis A virus IgG of packing antibody positive and negative reference substance, enzyme labeling bond, enzyme; And
7) be assembled into finished product.
7, method as claimed in claim 6 is characterized in that, described bag is by the step 2 of solid phase carrier) adopt following method:
1) bag quilt
With 0.1M pH value is the hepatitis A viral antigen coating buffer that 9.0 dipotassium hydrogen phosphate damping fluid is mixed with desired concn, and coating buffer is carried on the solid phase carrier;
2) sealing
With containing 2% BSA, 0.015% gelatin pH value is 6.8～7.3, and concentration is that the phosphate buffer of 0.01M seals above-mentioned solid phase carrier as confining liquid.
As claim 6 or 7 described methods, it is characterized in that 8, described bag is microwell plate, plastic bead, plastic tube or magnetic-particle by the solid phase carrier of hepatitis A viral antigen.
9, method as claimed in claim 6 is characterized in that, described enzyme is the hepatitis A virus antibody (resisting or monoclonal antibody) of alkaline phosphatase or horseradish peroxidase-labeled more; Described chemical luminous substrate is 1,2-two oxidative ethane analog derivatives, luminol or different luminol.
10, method as claimed in claim 9, it is characterized in that described 1,2-two oxidative ethane analog derivatives are (diamantane)-1,2-two oxidative ethanes, 3-(2 '-the spiral diamantane)-4-methoxyl-4-(3 " the phosphorus acyloxy) phenyl-1,2-two oxidative ethanes, CSPD or CDP-Star.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810102002A CN101533026A (en) | 2008-03-14 | 2008-03-14 | Chemoluminescent immunoassay kit of hepatitis A virus IgG antibody and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810102002A CN101533026A (en) | 2008-03-14 | 2008-03-14 | Chemoluminescent immunoassay kit of hepatitis A virus IgG antibody and preparation method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102072958A (en) * | 2010-11-09 | 2011-05-25 | 北京科美东雅生物技术有限公司 | Chemiluminescence immunoassay kit used for detecting aflatoxin B1 and preparation method and use method thereof |
| CN104597255A (en) * | 2015-02-04 | 2015-05-06 | 华中农业大学 | Test paper card for detecting pseudorabies virus gE protein antibody in porcine serum as well as preparation method and application thereof |
| CN105954510A (en) * | 2016-06-30 | 2016-09-21 | 深圳市亚辉龙生物科技股份有限公司 | Chemiluminescence immunodetection kit for anti-beta 2 glycoprotein I antibodies IgG and preparation method of kit |
-
2008
- 2008-03-14 CN CN200810102002A patent/CN101533026A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102072958A (en) * | 2010-11-09 | 2011-05-25 | 北京科美东雅生物技术有限公司 | Chemiluminescence immunoassay kit used for detecting aflatoxin B1 and preparation method and use method thereof |
| CN104597255A (en) * | 2015-02-04 | 2015-05-06 | 华中农业大学 | Test paper card for detecting pseudorabies virus gE protein antibody in porcine serum as well as preparation method and application thereof |
| CN105954510A (en) * | 2016-06-30 | 2016-09-21 | 深圳市亚辉龙生物科技股份有限公司 | Chemiluminescence immunodetection kit for anti-beta 2 glycoprotein I antibodies IgG and preparation method of kit |
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Owner name: BEIJING KEMEI BIOLOGICAL TECHNOLOGY CO., LTD. Free format text: FORMER OWNER: KEMEI DONGYA BIOLOGICAL TECHNOLOGY CO., LTD., BEIJING Effective date: 20111028 |
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Effective date of registration: 20111028 Address after: 100094 Beijing city Haidian District Yongfeng base Feng Xian Road No. 7 North Park Applicant after: Beijing Kemei Biological Technology Co., Ltd. Address before: 100094 Beijing city Haidian District Yongfeng base Feng Xian Road No. 7 North Park Applicant before: Kemei Dongya Biological Technology Co., Ltd., Beijing |
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Application publication date: 20090916 |