CN104316471B - Detect the system and method for guide mode resonance filter spectrum - Google Patents
Detect the system and method for guide mode resonance filter spectrum Download PDFInfo
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- CN104316471B CN104316471B CN201410632510.0A CN201410632510A CN104316471B CN 104316471 B CN104316471 B CN 104316471B CN 201410632510 A CN201410632510 A CN 201410632510A CN 104316471 B CN104316471 B CN 104316471B
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- beamlet
- mode resonance
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- wave filter
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Abstract
The invention discloses a kind of system and method for detecting guide mode resonance filter spectrum, its system includes：One light source, for launching a main beam；One beam splitter, the beam splitter is arranged at the light path of the main beam, for the main beam to be decomposed into one first beamlet and one second beamlet；One guide mode resonance wave filter, the guide mode resonance wave filter is arranged at the light path of first beamlet；One speculum, the speculum is arranged at the light path of second beamlet；One first spectroanalysis instrument and one second spectroanalysis instrument.As a result of a kind of system and method for detection guide mode resonance filter spectrum of the present invention, same place biological sample can be realized by the incident detection of different faces, greatly reduce and readjust light path and the again error caused by coating biological sample, have the advantages that simple operation, accuracy are high.
Description
Technical field
The present invention relates to field of biological pharmacy, more particularly to a kind of system for detecting guide mode resonance filter spectrum and side
Method.
Background technology
As guide mode resonance is increasingly widely applied in field of biological pharmacy, its accuracy of detection there has also been increasingly
High requirement.Detection generally requires to coat biological sample several times at present, so as to need, to biological sample, to be smeared repeatedly unavoidably
And wiping, it is highly prone to yet with the grating layer of guide mode resonance wave filter in the coating repeatedly of biological sample and wiping process
Damage；And testing result, also due to the adjustment of wave filter is different with biological sample dropwise addition and is affected, detection accuracy is low；
The operating process of detection is also increased simultaneously, increases the complexity of testing process；Cause the waste of biological sample.
The content of the invention
It is an object of the invention to overcome the defect of prior art, and provide a kind of detection guide mode resonance filter spectrum
System and method, can realize same place biological sample by the incident detection of different faces, greatly reduce readjust light path and
Again the error caused by coating biological sample, it is to avoid the waste of biological sample resource is high with simple operation, accuracy
Advantage.
Realizing the technical scheme of above-mentioned purpose is：
A kind of system of detection guide mode resonance filter spectrum of the present invention, including：
One light source, for launching a main beam；
One beam splitter, the beam splitter is arranged at the light path of the main beam, for the main beam to be decomposed into one
One beamlet and one second beamlet；
One guide mode resonance wave filter, the guide mode resonance wave filter is arranged at the light path of first beamlet；
One speculum, the speculum is arranged at the light path of second beamlet；
One first spectroanalysis instrument, first spectroanalysis instrument receives the first optical signal, wherein, first optical signal
Injected by first beamlet from the first face of the guide mode resonance wave filter and from the second of the guide mode resonance wave filter
Project to be formed in face；
One second spectroanalysis instrument, second spectroanalysis instrument receives the second optical signal, wherein, second optical signal
Inject and led from described from the second face of the guide mode resonance wave filter after being reflected by second beamlet through the speculum
Project to be formed in first face of mould resonance filter.
Further improvement of the present invention is, the guide mode resonance wave filter include stacking gradually a grating layer of laying,
One ducting layer and a basalis.
Further improvement of the present invention is, described also including one first fiber coupler and one second fiber coupler
First fiber coupler is arranged in the light path between the guide mode resonance wave filter and first spectroanalysis instrument, and described
Two fiber couplers are arranged in the light path between the guide mode resonance wave filter and second spectroanalysis instrument.
Further improvement of the present invention is, also including one the 3rd fiber coupler, and the 3rd fiber coupler is set
In light path between the light source and the beam splitter.
Further improvement of the present invention is that the light source uses amplified spontaneous emission source.
A kind of detection guide mode resonance filter spectrum of system based on present invention detection guide mode resonance filter spectrum
Method, including step：
S1：Open the light source and send the main beam；
S2：The main beam is decomposed into by first beamlet and second beamlet by the beam splitter；
S3：Regulation is coated with the guide mode resonance wave filter of biological sample so that first beamlet passes through described
Biological sample, and first beamlet injects from the face of guide mode resonance wave filter first and from the guide mode resonance wave filter
The second face project to form first optical signal；
S4：Adjust the speculum so that pass through the second beamlet of speculum reflection by the biological sample,
And second beamlet is injected from the face of guide mode resonance wave filter second and from the first face of the guide mode resonance wave filter
Injection forms second optical signal；
S5：First spectroanalysis instrument receives first optical signal and is monitored in real time and record the first light letter
Number spectral information；Second spectroanalysis instrument receives second optical signal and is monitored in real time and record second light
The spectral information of signal.
Further improvement of the present invention is, in the step S5, and first optical signal passes through the first fiber coupling
Received after device by first spectroanalysis instrument, wherein, first fiber coupler is arranged at the guide mode resonance wave filter
With the light path between first spectroanalysis instrument；Second optical signal by after second fiber coupler by described
Two spectroanalysis instruments are received, and second fiber coupler is arranged at the guide mode resonance wave filter and second spectrum analysis
Light path between instrument.
Further improvement of the present invention is, in the step S2, and the main beam passes through the 3rd fiber coupling
Decomposed after device by the beam splitter, the 3rd fiber coupler is arranged at light path between the light source and the beam splitter.
Further improvement of the present invention is that it is saturating that the spectral information includes crest location information, wavelength information and spectrum
Cross rate data；Also include step after the step S5：
S6：By contrasting the data of the wavelength information and the spectral transmittance, the first optical signal of analysis and the second light
The difference of the formant of signal, the influence of analysis the first beamlet and the second beamlet to guide mode resonance filter transmission spectrum.
The present invention makes it have following beneficial effect as a result of above technical scheme：
Light source be used for provide detection light source, beam splitter be used for main beam is decomposed into two beams, with obtain the first beamlet and
Second beamlet；Guide mode resonance wave filter is used to carry detected biological sample and to incident the first beamlet and the second sub-light
Shu Jinhang filterings obtain the first optical signal and the second optical signal；It is narrow because guide mode resonance wave filter has filtering spectrum efficiency high
The characteristic of bandwidth, can be used for the association reaction of test biology sample antibody antigen.Speculum is used to change the second beamlet
Propagation path so that the second beamlet after reflection is injected from the face of guided mode resonance filter second, coordinates guide mode resonance wave filter
Be arranged at the first sub-light beam optical path, realize the first beamlet and the second ground light beam respectively from the first face of guided mode resonance filter and
Second face is injected, thus realize once to be coated on guide mode resonance wave filter biological sample positive and negative two sides while incident examine
Survey, it is to avoid error caused by coating biological sample and adjustment light path again, simplify the flow of detection and improve detection
Accuracy.First spectroanalysis instrument and the second spectroanalysis instrument are used to receive the first optical signal and the second optical signal, to being detected
Biological sample is detected in real time.First fiber coupler and the second fiber coupler are used for the collimator and extender of light beam.
Brief description of the drawings
Fig. 1 detects the structural representation of the system of guide mode resonance filter spectrum for the present invention；
Fig. 2 detects the flow chart of the method for guide mode resonance filter spectrum for the present invention.
Embodiment
With reference to specific embodiment, the invention will be further described.
Referring to Fig. 1, a kind of system of detection guide mode resonance filter spectrum of the present invention, including：One light source 1, one point
Beam mirror 2, a guide mode resonance wave filter 3, a speculum 4, one first spectroanalysis instrument 51, one second spectroanalysis instrument 52,1
One fiber coupler 61, one second fiber coupler 62 and one the 3rd fiber coupler 63.
Wherein light source 1, (the ASE light of amplified spontaneous emission source 1 is used for launching light source 1 in a main beam, the present embodiment
Source).Beam splitter 2 is arranged at the light path of main beam, for main beam to be decomposed into one first beamlet and one second beamlet.This
In embodiment, the 3rd fiber coupler 63 is additionally provided between beam splitter 2 and light source 1.
Guide mode resonance wave filter 3 is arranged at the light path of the first beamlet.Guide mode resonance wave filter 3 is used and led in the present embodiment
Mould resonance filter.Guide mode resonance wave filter includes the grating layer, a ducting layer and a basalis for stacking gradually laying.
Speculum 4 is arranged at the light path of the second beamlet.First beamlet is injected from the first face of guided mode resonance filter 3
And project to form one first optical signal from the second face of guided mode resonance filter 3；Self-conductance after the reflected reflection of mirror 4 of second beamlet
Second face of mould resonance filter 3 is injected and projects to form one second optical signal from the first face of guided mode resonance filter 3.This reality
The first face for applying guide mode resonance wave filter 3 in example is grating layer, and the second face of guide mode resonance wave filter 3 is basalis.At other
In embodiment, also the first face of guide mode resonance wave filter 3 can be set to basalis, by the second face of guide mode resonance wave filter 3
It is set to grating layer.
First spectroanalysis instrument 51 receives the first optical signal；Second spectroanalysis instrument 52 receives the second optical signal.This implementation
In example the first fiber coupler 61 is provided between the spectroanalysis instrument 51 of guide mode resonance wave filter 3 and first；Guide mode resonance is filtered
The second fiber coupler 62 is provided between the spectroanalysis instrument 52 of device 3 and second.
It can realize that the biological sample of same position point on guide mode resonance wave filter 3 is entered by difference by the system of the present invention
Penetrate the detection of the spectral characteristic of mode.Incident beam by the different planes of incidence be light source 1 through beam splitter 2 points be the beam of identical two
Light.It is another coupled to wave filter is incided after a spectroanalysis instrument and reflected mirror 4 that the two-beam incides wave filter one side respectively
Simultaneously it is coupled to another spectroanalysis instrument., can be by analyzing the difference of formant, so as to analyze light beam by the system of the present invention
Influence by positive and negative when incident to the detection spectrum of guide mode resonance wave filter 3 respectively.Biology can be realized using spectroanalysis instrument
The dynamic monitoring of sample characteristics of for example, realizes the real-time of detection.
In addition, such as by the position that adjusts incidence end or upset wave filter realized by azimuthal change by just,
The minor adjustment of incident end position or guide mode resonance wave filter 3 can all be led in the spectrum of reverse side detection guide mode resonance wave filter 3, light path
The difference of peak is caused, so as to influence the result detected.The present invention is filtered by beam splitter 2, speculum 4 and guide mode resonance
The light path of ripple device 3 coordinates, and the position or upset guide mode resonance wave filter 3 without adjusting incidence end are changed i.e. by azimuthal
It can be achieved to detect the spectrum of guide mode resonance wave filter 3 by positive and negative, so as to improve detection accuracy.
Referring to Fig. 2, a kind of method of detection guide mode resonance filter spectrum based on present system, including step：
S1：Open light source and send main beam；
S2：Main beam is decomposed into by the first beamlet and the second beamlet by beam splitter；In the present embodiment, main beam leads to
Decomposed after crossing the 3rd fiber coupler by beam splitter, the 3rd fiber coupler is arranged at light path between light source and beam splitter.
S3：Regulation is coated with the guide mode resonance wave filter of biological sample so that the first beamlet is by biological sample, and the
One beamlet is injected from the face of guide mode resonance wave filter first and projects to form the first light letter from the second face of guided mode resonance filter
Number；
S4：Adjust speculum so that the second beamlet reflected by speculum is by biological sample, and the second beamlet
Injected from the face of guide mode resonance wave filter second and project to form the second optical signal from the first face of guided mode resonance filter；
S5：First spectroanalysis instrument receives the spectrum letter that the first optical signal is monitored and records the first optical signal in real time
Breath；Second spectroanalysis instrument receives the second optical signal and is monitored in real time and record the spectral information of the second optical signal.This implementation
Example in, the first optical signal by after the first fiber coupler by the first spectroanalysis instrument receive, wherein, the first fiber coupler is set
The light path being placed between guide mode resonance wave filter and the first spectroanalysis instrument；Second optical signal passes through quilt after the second fiber coupler
Second spectroanalysis instrument is received, and the second fiber coupler is arranged at the light between guide mode resonance wave filter and the second spectroanalysis instrument
Road.In the present embodiment, spectral information includes peak-location information, wavelength information and spectral transmittance data.
S6：By contrasting the wavelength information and spectral transmittance data, first optical signal and the second light letter are analyzed
Number formant difference, the influence to guide mode resonance filter transmission spectrum of analysis the first beamlet and the second beamlet.
In the present embodiment, the main beam sent by light source reaches beam splitter, main beam warp after the 3rd fiber coupler
It is divided into identical two-beam, i.e. the first beamlet and the second beamlet after beam splitter.Wherein the first beamlet, arrival is placed with biology
The somewhere of the grating layer (or basalis) of the guide mode resonance wave filter of sample, transmitted through guide mode resonance wave filter through the first optical fiber coupling
After clutch, real-time monitoring and spectra re-recorded information that the first spectroanalysis instrument carries out peak value are delivered to；And the second beamlet, reach anti-
Mirror is penetrated, the same place of the basalis (or grating layer) of guide mode resonance wave filter is reached after reflection, through the second optical fiber coupling after transmission
After clutch, real-time monitoring and spectra re-recorded information that the second spectroanalysis instrument carries out peak value are delivered to.This makes it possible to avoid guided mode
The error that the movement of resonance filter is caused, and ensure that incidence point and receiving point are in same position under different incidences.
By the method for the present invention, it can realize that same position biological sample is incident by different faces by a detection light path
Detection, will not by because the adjustment of wave filter is different with biological sample coating and caused by influenceed.Change is only needed to be connected to
The coupling optical fiber of the receiving terminal of spectroanalysis instrument, so that it may greatly reduce to readjust light path and coat biological sample again and drawn
The error risen.Meanwhile, effectively save biological sample resource.It not only avoid the mistake that test point is not caused in same position
Difference, and biological sample twice need not be added dropwise again could realize that biological sample is coated in the detection of wave filter different faces, no
The accuracys for being influenceed, improving test different with biological sample dropwise addition can be adjusted by guide mode resonance wave filter, can also be more
The reaction and change of biological sample are detected exactly.
In addition, the method for the present invention is combined in protein-protein, receptor agents and analyte are combined, small molecule screening
Deng application in equally play an important role.
The present invention is described in detail above in association with accompanying drawing embodiment, those skilled in the art can be according to upper
State it is bright the present invention is made many variations example.Thus, some of embodiment details should not constitute limitation of the invention, this
Invention regard the scope defined using appended claims as protection scope of the present invention.
Claims (4)
1. a kind of method for detecting guide mode resonance filter spectrum, it is characterised in that detection guide mode resonance filter spectrum is
System includes：
One light source, for launching a main beam；
One beam splitter, the beam splitter is arranged at the light path of the main beam, for the main beam to be decomposed into one first son
Light beam and one second beamlet；
One guide mode resonance wave filter, the guide mode resonance wave filter is arranged at the light path of first beamlet；
One speculum, the speculum is arranged at the light path of second beamlet；
One first spectroanalysis instrument, first spectroanalysis instrument receives the first optical signal, wherein, the first optical signals institute
The first beamlet is stated to inject and penetrate from the second face of the guide mode resonance wave filter from the first face of the guide mode resonance wave filter
Go out to be formed；
One second spectroanalysis instrument, second spectroanalysis instrument receives the second optical signal, wherein, the second optical signals institute
State and injected after the second beamlet reflects through the speculum from the second face of the guide mode resonance wave filter and common from the guided mode
Project to be formed in first face of polarization filter；
Methods described, including step：
S1：Open the light source and send the main beam；
S2：The main beam is decomposed into by first beamlet and second beamlet by the beam splitter；
S3：Regulation is coated with the guide mode resonance wave filter of biological sample so that first beamlet passes through the biology
Sample, and first beamlet injects from the face of guide mode resonance wave filter first and from the of the guide mode resonance wave filter
Project to form first optical signal in two faces；
S4：Adjust the speculum so that the second beamlet reflected by the speculum is made by the biological sample
Second beamlet and the first beamlet are incident on the same place of biological sample, and second beamlet is from the guided mode
The face of resonance filter second is injected and projects to form second optical signal from the first face of the guide mode resonance wave filter；
S5：First spectroanalysis instrument receives first optical signal and is monitored in real time and record first optical signal
Spectral information；Second spectroanalysis instrument receives second optical signal and is monitored in real time and record second optical signal
Spectral information.
2. according to the method described in claim 1, it is characterised in that：In the step S5, first optical signal passes through
Received after one fiber coupler by first spectroanalysis instrument, wherein, first fiber coupler is arranged at the guided mode
Light path between resonance filter and first spectroanalysis instrument；Second optical signal passes through second fiber coupler
Received afterwards by second spectroanalysis instrument, second fiber coupler is arranged at the guide mode resonance wave filter and described the
Light path between two spectroanalysis instruments.
3. method according to claim 2, it is characterised in that：In the step S2, the main beam passes through the 3rd light
Decomposed after fine coupler by the beam splitter, the 3rd fiber coupler is arranged at light between the light source and the beam splitter
Lu Shang.
4. method according to claim 3, it is characterised in that：The spectral information, which includes crest location information, wavelength, to be believed
Breath and spectrum percent of pass data；Also include step after the step S5：
S6：By contrasting the first optical signal and the second optical signal described in the wavelength information and the spectrum percent of pass data analysis
Formant difference, the influence to guide mode resonance filter transmission spectrum of analysis the first beamlet and the second beamlet.
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| CN201410632510.0A CN104316471B (en) | 2014-11-11 | 2014-11-11 | Detect the system and method for guide mode resonance filter spectrum |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050068543A1 (en) * | 2003-01-24 | 2005-03-31 | General Dynamics Advanced Information Systems, Inc. | System and method for detecting presence of analytes using gratings |
| CN101008609A (en) * | 2006-01-27 | 2007-08-01 | 财团法人工业技术研究院 | Optical waveguide biological detecting device |
| CN103969185A (en) * | 2014-05-19 | 2014-08-06 | 上海理工大学 | Method for improving test sensitivity of guided mode resonance biosensor |
| CN104101928A (en) * | 2014-07-25 | 2014-10-15 | 上海理工大学 | Beam splitter with continuous splitting ratios |
-
2014
- 2014-11-11 CN CN201410632510.0A patent/CN104316471B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050068543A1 (en) * | 2003-01-24 | 2005-03-31 | General Dynamics Advanced Information Systems, Inc. | System and method for detecting presence of analytes using gratings |
| CN101008609A (en) * | 2006-01-27 | 2007-08-01 | 财团法人工业技术研究院 | Optical waveguide biological detecting device |
| CN103969185A (en) * | 2014-05-19 | 2014-08-06 | 上海理工大学 | Method for improving test sensitivity of guided mode resonance biosensor |
| CN104101928A (en) * | 2014-07-25 | 2014-10-15 | 上海理工大学 | Beam splitter with continuous splitting ratios |
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