CN104313107A - A viability identification method for pineapple pollen - Google Patents
A viability identification method for pineapple pollen Download PDFInfo
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- CN104313107A CN104313107A CN201410502446.4A CN201410502446A CN104313107A CN 104313107 A CN104313107 A CN 104313107A CN 201410502446 A CN201410502446 A CN 201410502446A CN 104313107 A CN104313107 A CN 104313107A
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Abstract
The invention discloses a viability identification method for pineapple pollen, and belongs to the technical field of identification of plant pollen viability. The method includes: (1) culture liquid preparation; (2) pollen culture device designing; (3) germination and culturing of the pollen; (4) observation, numerical statement and selection of germinated pollen; and other process steps. The method has characteristics of simple equipment, convenient and rapid method, and capability of being visual, accurate and reliable.
Description
Technical field
The present invention relates to the authenticate technology field of plant pollen vigor, be specifically related to a kind of method identifying pineapple Pollen Activity.
Background technology
The collection of pollen, storage and the qualification to its vigor are the important steps of plant hybridization breeding, and the height of Pollen Activity have impact on the success ratio of selfing and hybridization greatly.The method of current qualification Pollen Activity has multiple, mainly contains microscope form observation method, triphenyltetrazolium chloride staining (TTC staining) and IKI staining (I-KI staining), germination in vitro method etc.Wherein germination in vitro method measures the simplest, the reliable method of Pollen Activity.Because pollen germination rate is the important indicator weighing pollen viability situation, the length of pollen tube also reflects the vitality situation of this pollen to a certain extent simultaneously.Current germination in vitro method all adopts solid medium, and need steps such as carrying out solution preparation, packing, sterilizing, solidify, process comparatively bothers, length consuming time, and must prepare in advance.
Summary of the invention
The present invention seeks to, have that solid medium need be prepared in advance for existing in-vitro pollen germination method, packing, sterilizing, the step such as to solidify, the defect such as cumbersome, length consuming time, propose a kind of simple, fast, the method for precise Identification pineapple Pollen Activity.
The object of the invention is achieved by the following technical programs:
Identify a method for pineapple Pollen Activity, the method is carried out as follows:
(1) prepare nutrient solution: in every 100ml distilled water, add 20g sucrose and 5-10mg boric acid, to mix and after adjusting ph to 6.4-6.6, for subsequent use;
(2) in glass culture dish, first flat crouching places common slide glass 1, then is poured into by nutrient solution in culture dish to the end face just having flooded slide glass;
(3) even on slide glass, loose sprinkle adopt after preserve the pineapple pollen of 0-24h after, cover the lid of glass culture dish, in 20-30 DEG C of room temperature leave standstill 2-4 hour;
(4) observe the sprouting situation of pollen in 5-7 the visual field under slide glass being positioned over 20 power microscopes, statistics obtains the average germination rate of pollen, filters out the lot sample pollen of pollen germination rate at more than 50%-90%.
The invention has the beneficial effects as follows:
1, to pineapple Pollen Activity, the present invention identifies that changing conventional solid medium is liquid culture method, and the preparation of this nutrient solution is simple, consuming time, the complicated step such as does not need through sterilizing, cools, solidifies, can be used while allocating; Culture dish used, slide glass etc. are all the ordinary articles in laboratory, more convenient;
2, flat sleeping placement one slide glass in culture dish, was both beneficial to microscopic examination, pollen can be made again to be unlikely and sink to impact sprouting bottom nutrient solution;
3, the present invention can add up by observing the sprouting situation of pollen, identify the vigor of pineapple pollen, have reliably, intuitively, feature efficiently.
Accompanying drawing explanation
The device schematic diagram of Fig. 1 pineapple Pollen Activity qualification
Wherein, 1-pollen, 2-slide glass, 3-glass culture dish, 4-nutrient solution
The side schematic view of Fig. 2 pineapple Pollen Activity identification apparatus
Wherein, 1-pollen, 2-slide glass, 3-glass culture dish, 4-nutrient solution
Embodiment
By following examples, also the present invention is described in further detail by reference to the accompanying drawings, but content of the present invention is not limited thereto.
Explanation to reagent involved by embodiment and instrument:
Microscope is the Leica M205C that Lycra company produces
Embodiment 1:
Identify a method for pineapple Pollen Activity, the method is carried out as follows:
(1) prepare nutrient solution: in every 100ml distilled water, add 20g sucrose and 7.5mg boric acid, to mix and after adjusting ph to 6.4, for subsequent use;
(2) in glass culture dish, first flat crouching places common slide glass 1, then is poured into by nutrient solution in culture dish to the end face just having flooded slide glass;
(3) even on slide glass, loose sprinkle adopt after preserve the pineapple pollen of 5h after, cover the lid of glass culture dish, in 25 DEG C of room temperatures leave standstill 3 hours;
(4) observe the sprouting situation of pollen in 5 visuals field under slide glass being positioned over 20 power microscopes, statistics obtains the average germination rate of pollen, filters out the lot sample pollen of the average germination rate of pollen more than 90%.
Embodiment 2:
(1) prepare nutrient solution: in every 100ml distilled water, add 20g sucrose and 5mg boric acid, to mix and after adjusting ph to 6.5, for subsequent use;
(2) in glass culture dish, first flat crouching places common slide glass 1, then is poured into by nutrient solution in culture dish to the end face just having flooded slide glass;
(3) even on slide glass, loose sprinkle adopt after preserve the pineapple pollen of 14h after, cover the lid of glass culture dish, in 20 DEG C of room temperatures leave standstill 4 hours;
(4) observe the sprouting situation of pollen in 6 visuals field under slide glass being positioned over 20 power microscopes, statistics obtains the average germination rate of pollen, filters out the lot sample pollen of pollen germination rate more than 75%.
Embodiment 3:
(1) prepare nutrient solution: in every 100ml distilled water, add 20g sucrose and 10mg boric acid, to mix and after adjusting ph to 6.6, for subsequent use;
(2) in glass culture dish, first flat crouching places common slide glass 1, then is poured into by nutrient solution in culture dish to the end face just having flooded slide glass;
(3) even on slide glass, loose sprinkle adopt after preserve the pineapple pollen of 24h after, cover the lid of glass culture dish, in 30 DEG C of room temperatures leave standstill 2 hours;
(4) observe the sprouting situation of pollen in 7 visuals field under slide glass being positioned over 20 power microscopes, statistics obtains the average germination rate of pollen, filters out the lot sample pollen of pollen germination rate more than 50%.
Claims (1)
1. identify a method for pineapple Pollen Activity, it is characterized in that the method is carried out as follows:
(1) prepare nutrient solution: in every 100ml distilled water, add 20g sucrose and 5-10mg boric acid, to mix and after adjusting ph to 6.4-6.6, for subsequent use;
(2) in glass culture dish, first flat crouching places common slide glass 1, then is poured into by nutrient solution in culture dish to the end face just having flooded slide glass;
(3) even on slide glass, loose sprinkle adopt after preserve the pineapple pollen of 0-24h after, cover the lid of glass culture dish, in 20-30 DEG C of room temperature leave standstill 2-4 hour;
(4) observe the sprouting situation of pollen and the length of pollen tube under slide glass being positioned over 20 power microscopes, and add up, filter out the lot sample pollen of pollen germination rate at more than 50%-90%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410502446.4A CN104313107B (en) | 2014-09-26 | 2014-09-26 | A kind of method identifying Fructus Ananadis comosi Pollen Activity |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410502446.4A CN104313107B (en) | 2014-09-26 | 2014-09-26 | A kind of method identifying Fructus Ananadis comosi Pollen Activity |
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| CN104313107A true CN104313107A (en) | 2015-01-28 |
| CN104313107B CN104313107B (en) | 2016-06-29 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114891630A (en) * | 2022-04-26 | 2022-08-12 | 南京林业大学 | System and method for detecting vitality of cerasus campanulata pollen |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101717747A (en) * | 2009-12-04 | 2010-06-02 | 西北农林科技大学 | Liquid medium for vitro scutellaria root pollen germination and method for testing activity of scutellaria root pollen |
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2014
- 2014-09-26 CN CN201410502446.4A patent/CN104313107B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101717747A (en) * | 2009-12-04 | 2010-06-02 | 西北农林科技大学 | Liquid medium for vitro scutellaria root pollen germination and method for testing activity of scutellaria root pollen |
Non-Patent Citations (4)
| Title |
|---|
| PARTON E ET AL.: "viability and storage of bromeliad pollen", 《EUPHYTICA》, vol. 125, no. 2, 31 May 2002 (2002-05-31), pages 155 - 161 * |
| 沈晓岚等: "不同培养条件对凤梨花粉离体萌发的影响", 《浙江农业科学》, no. 02, 11 April 2008 (2008-04-11), pages 168 - 1 * |
| 王友保等: "花粉萌发与花粉管生长实验的改进", 《生物学通报》, vol. 41, no. 12, 20 December 2006 (2006-12-20) * |
| 陈学森: "《植物育种学实验》", 30 June 2004, article "植物花粉生活力测定技术", pages: 21 - 13-26 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114891630A (en) * | 2022-04-26 | 2022-08-12 | 南京林业大学 | System and method for detecting vitality of cerasus campanulata pollen |
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