CN104293700A - Production process of bacillus licheniformis for feed - Google Patents
Production process of bacillus licheniformis for feed Download PDFInfo
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- CN104293700A CN104293700A CN201410480613.XA CN201410480613A CN104293700A CN 104293700 A CN104293700 A CN 104293700A CN 201410480613 A CN201410480613 A CN 201410480613A CN 104293700 A CN104293700 A CN 104293700A
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- bacillus licheniformis
- feed
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- production technique
- fermentation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention discloses a production process of bacillus licheniformis for a feed. The production process comprises the following steps: (1) inoculating bacillus licheniformis onto a culture medium for fermentation; (2) performing double-effect evaporation on a fermentation liquid obtained in the step (1), thereby obtaining a strong fungus liquid; and (3) feeding the strong fungus liquid obtained in the step (2) into a spray dryer for spray drying, thereby obtaining a product. The production process has the characteristics of simple production process, high nutrient substance utilization rate, small environment pollution and good product effect.
Description
Technical field
The invention belongs to feed additive field, be specifically related to the production technique of a kind of feed with Bacillus licheniformis.
Background technology
Bacillus licheniformis has stronger proteolytic enzyme, lipase, diastatic activity, the degraded of feed Middle nutrition element can be promoted, stimulate the growth of aquatic animal immune organ, enhancing body immunizing power can produce activity resistent material enzyme. and the biology with uniqueness takes oxygen mechanism of action by force, the growth and breeding of pathogenic bacterium can be suppressed, make aquatic product animal more abundant to absorbing of feed.
Existing general production process is that bacterial classification is fermented under certain condition, then thalline is separated from fermented liquid, finally thalline be loaded into carrier carries out drying after obtain product.The shortcoming of this type of technological process is: product is viable bacteria mainly, isolates a large amount of nutritive substance in the fermented liquid after thalline and activeconstituents and the thalline that is not completely segregated out can not get effective utilization.If the patent No. is the patent of CN103848911A, passed through after filter press or ceramic membrane filter by cecropin antimicrobial peptides fermented liquid, the macro-nutrients not only in fermented liquid is filtered, and facility investment is comparatively large, complex process.
Summary of the invention
The object of the invention is in order to the thalline making full use of a large amount of nutritive substance in fermented liquid and activeconstituents and live, provide a kind of output high, production technique is simple, low in the pollution of the environment, the production technique of the Bacillus licheniformis of good product effect.
For achieving the above object, the present invention adopts following technical proposals:
A feed production technique for Bacillus licheniformis, comprises the following steps:
(1) inoculation medium of Bacillus licheniformis ferments;
(2) fermented liquid that step (1) obtains is carried out double-effect evaporation, obtain bacterium dope;
(3) the bacterium dope that step (2) obtains is sent in spray-dryer carries out spraying dry and obtain product.
Substratum composition described in step (1) is W-Gum 2-4%, dregs of beans 2-6%, peptone 1-3%, magnesium sulfate 1%, manganous sulfate 0.04%, potassium primary phosphate 1%, ferrous sulfate 0.01-0.05%, calcium carbonate 0.6% by mass percentage.
The inoculum size of Bacillus licheniformis be substratum quality 1%, leavening temperature 36-37 DEG C, fermentation tank pressure: 0.01-0.03MPa, fermentation air flow 1: 1, fermentation mixing speed 150 revs/min, ph value 6-8.
Double-effect evaporation in described step (2), the temperature of evaporator room is 65-75 DEG C, vacuum tightness is-0.05 MPa ~-0.065MPa.
Spray-dryer described in described step (3), the inlet temperature of spray-dryer tower 150 DEG C-160 DEG C, temperature 80 DEG C-90 DEG C in tower, vacuum-0.01 MPa ~-0.03 MPa, air outlet temperature 70 DEG C-80 DEG C.
In described step (2), the solid content obtaining bacterium dope after double-effect evaporation is 15%-25%.
The beneficial effect reached by above production technique is as follows: general production technique is fermented liquid-separation-thalline-carrier-drying-product.The technique that the present invention adopts is fermented liquid-double-effect evaporation-spraying dry-product, and production technique is simplified.The product mainly viable bacteria of original technique, isolates a large amount of nutritive substance in the fermented liquid after thalline and the viable bacteria that is not completely segregated is effectively used.The existing viable bacteria of product prepared by the present invention has again activeconstituents in fermented liquid and nutritive substance, and this production process decreases the pollution of fermented liquid to environment, the good product effect of production.
Embodiment
embodiment 1
A feed production technique for Bacillus licheniformis, comprises the following steps:
(1) Bacillus licheniformis is inoculated on substratum ferments, nutrient media components is as follows by mass percentage: W-Gum 2-4%, dregs of beans 2-6%, peptone 1-3%, magnesium sulfate 1%, manganous sulfate 0.04%, potassium primary phosphate 1%, ferrous sulfate 0.01-0.05%, calcium carbonate 0.6%, and all the other are soft water.The inoculum size of Bacillus licheniformis be substratum quality 1%, leavening temperature 36-37 DEG C, fermentation tank pressure 0.01-0.03MPa, fermentation air flow 1: 1, fermentation stir speed (S.S.): 150 revs/min, ph value 6-8.
(2) fermented liquid that step (1) obtains is carried out double-effect evaporation, obtain bacterium dope.The temperature of the evaporator room of double-effect evaporation is 65-75 DEG C, vacuum tightness is-0.05 MPa ~-0.065MPa.(3) the bacterium dope that step (2) obtains is sent in spray-dryer carries out spraying dry and obtain product.Inlet temperature in the dryer column of spray-dryer 150 DEG C-160 DEG C, temperature 80 DEG C-90 DEG C in tower, vacuum tightness-0.01 MPa ~-0.03 MPa, air outlet temperature 70 DEG C-80 DEG C.The solid content obtaining bacterium dope after double-effect evaporation is 15%-25%.
embodiment 2
A feed production technique for Bacillus licheniformis, comprises the following steps:
(1) Bacillus licheniformis is inoculated on substratum ferments, nutrient media components is as follows by mass percentage: W-Gum 2%, dregs of beans 6%, peptone 3%, magnesium sulfate 1%, manganous sulfate 0.04%, potassium primary phosphate 1%, ferrous sulfate 0.01%, calcium carbonate 0.6%, and all the other are soft water.The inoculum size of Bacillus licheniformis be substratum quality 1%, leavening temperature 36 DEG C, fermentation tank pressure 0.03MPa, fermentation air flow 1: 1, fermentation turn stir speed (S.S.) 150 revs/min, ph value 8.
(2) fermented liquid that step (1) obtains is carried out double-effect evaporation, obtain bacterium dope.The temperature of double-effect evaporation room is 65 DEG C, vacuum tightness is-0.06 MPa.
(3) the bacterium dope that step (2) obtains is sent in spray-dryer carries out spraying dry and obtain product.Inlet temperature in the dryer column of spray-dryer 160 DEG C, temperature 89 DEG C in tower, vacuum tightness-0.01MPa, air outlet temperature 71 DEG C.The solid content obtaining bacterium dope after double-effect evaporation is 15.9%.
embodiment 3
Step is identical with embodiment 1, and difference is the substratum composition described in step (1) is W-Gum 4%, dregs of beans 6%, peptone 2%, magnesium sulfate 1%, manganous sulfate 0.04%, potassium primary phosphate 1%, ferrous sulfate 0.04%, calcium carbonate 0.6% by mass percentage.
The inoculum size of Bacillus licheniformis be substratum quality 1%, leavening temperature 37 DEG C DEG C, fermentation tank pressure 0.01MPa, fermentation air flow 1: 1, fermentation turn stir speed (S.S.) 150 revs/min, ph value 7.
Double-effect evaporation in step (2), the temperature of evaporator room is 75 DEG C, vacuum tightness is-0.05MPa.
Spray-drying tower described in step (3), the inlet temperature of spray-drying tower 156.7 DEG C, temperature 82 DEG C in tower, vacuum tightness-0.02MPa, air outlet temperature 80 DEG C.
In step (2), the solid content obtaining bacterium dope after double-effect evaporation is 25%.
embodiment 4
Step is identical with embodiment 1, and difference is the substratum composition described in step (1) is W-Gum 2%, dregs of beans 2%, peptone 1%, magnesium sulfate 1%, manganous sulfate 0.04%, potassium primary phosphate 1%, ferrous sulfate 0.05%, calcium carbonate 0.6% by mass percentage.
The inoculum size of Bacillus licheniformis be substratum quality 1%, leavening temperature 37 DEG C, fermentation tank pressure 0.01MPa, fermentation air flow 1: 1, fermentation turn stir speed (S.S.) 150 revs/min, ph value 6.
Double-effect evaporation in step (2), the temperature of evaporator room is 73 DEG C, vacuum tightness is-0.05MPa.
Spray-dryer described in step (3), the inlet temperature of spray-dryer tower 158.8 DEG C, temperature 90 DEG C in tower, vacuum tightness-0.03MPa, air outlet temperature 78 DEG C.
In step (2), the solid content obtaining bacterium dope after double-effect evaporation is 15%.
embodiment 5
Step is identical with embodiment 1, and difference is the substratum composition described in step (1) is W-Gum 4%, dregs of beans 6%, peptone 3%, magnesium sulfate 1%, manganous sulfate 0.04%, potassium primary phosphate 1%, ferrous sulfate 0.05%, calcium carbonate 0.6% by mass percentage.
The inoculum size of Bacillus licheniformis be substratum quality 1%, leavening temperature 37 DEG C, fermentation tank pressure 0.01MPa, fermentation air flow 1: 1, fermentation rotating speed 150 revs/min, ph value 6.
Double-effect evaporation in step (2), the temperature of evaporator room is 69.8 DEG C, vacuum tightness is-0.05MPa.
Spray-dryer described in step (3), the inlet temperature of spray-dryer tower 151 DEG C, temperature 86.2 DEG C in tower, vacuum tightness-0.01MPa, air outlet temperature 77 DEG C.
In step (2), the solid content obtaining bacterium dope after double-effect evaporation is 23.1%.
embodiment 6
Step is identical with embodiment 1, and difference is the substratum composition described in step (1) is W-Gum 3%, dregs of beans 6%, peptone 1%, magnesium sulfate 1%, manganous sulfate 0.04%, potassium primary phosphate 1%, ferrous sulfate 0.05%, calcium carbonate 0.6% by mass percentage.
The inoculum size of Bacillus licheniformis be substratum quality 1%, leavening temperature 37 DEG C, fermentation tank pressure 0.02MPa, fermentation air flow 1: 1, fermentation rotating speed 150 revs/min, ph value 7.
Double-effect evaporation in step (2), the temperature of evaporator room is 71 DEG C, vacuum tightness is-0.05MPa.
Spray-dryer described in step (3), the inlet temperature of spray-dryer tower 150 DEG C, temperature 90 DEG C in tower, vacuum tightness-0.01MPa, air outlet temperature 80 DEG C.
In step (2), the solid content obtaining bacterium dope after double-effect evaporation is 24.8%.
embodiment 7
Step is identical with embodiment 1, and difference is the substratum composition described in step (1) is W-Gum 4%, dregs of beans 4%, peptone 2%, magnesium sulfate 1%, manganous sulfate 0.04%, potassium primary phosphate 1%, ferrous sulfate 0.03%, calcium carbonate 0.6% by mass percentage.
The inoculum size of Bacillus licheniformis be substratum quality 1%, leavening temperature 37 DEG C DEG C, fermentation tank pressure 0.03MPa, fermentation air flow 1: 1, fermentation rotating speed 150 revs/min, ph value 6.
Double-effect evaporation in step (2), the temperature of evaporator room is 73 DEG C, vacuum tightness is-0.06MPa.
Spray-dryer described in step (3), the inlet temperature of spray-dryer tower 152 DEG C, temperature 88.5 DEG C in tower, vacuum tightness-0.03MPa, air outlet temperature 75.9 DEG C.
In step (2), the solid content obtaining bacterium dope after double-effect evaporation is 19.5%.
embodiment 8
Step is identical with embodiment 1, and difference is the substratum composition described in step (1) is W-Gum 4%, dregs of beans 6%, peptone 3%, magnesium sulfate 1%, manganous sulfate 0.04%, potassium primary phosphate 1%, ferrous sulfate 0.05%, calcium carbonate 0.6% by mass percentage.
The inoculum size of Bacillus licheniformis be substratum quality 1%, leavening temperature 37 DEG C, fermentation tank pressure 0.03MPa, fermentation air flow 1: 1, fermentation rotating speed 150 revs/min, ph value 7.5.
Double-effect evaporation in step (2), the temperature of evaporator room is 67 DEG C, vacuum tightness is-0.05MPa.
Spray-dryer described in step (3), the inlet temperature of spray-dryer tower 153 DEG C, temperature 84 DEG C in tower, vacuum tightness-0.01MPa, air outlet temperature 80 DEG C.
In step (2), the solid content obtaining bacterium dope after double-effect evaporation is 20.7%.
embodiment 9
Step is identical with embodiment 1, and difference is the substratum composition described in step (1) is W-Gum 3%, dregs of beans 5%, peptone 2%, magnesium sulfate 1%, manganous sulfate 0.04%, potassium primary phosphate 1%, ferrous sulfate 0.03%, calcium carbonate 0.6% by mass percentage.
The inoculum size of Bacillus licheniformis be substratum quality 1%, leavening temperature 37 DEG C, fermentation tank pressure 0.01MPa, fermentation air flow 1: 1, fermentation rotating speed 150 revs/min, ph value 6.
Double-effect evaporation in step (2), the temperature of evaporator room is 71.8 DEG C, vacuum tightness is-0.05MPa.
Spraying dry described in step (3), the inlet temperature of spray-drying tower 157 DEG C, temperature 95 DEG C in tower, vacuum tightness-0.02MPa, air outlet temperature 70 DEG C.
In step (2), the solid content obtaining bacterium dope after double-effect evaporation is 24.5%.
Claims (6)
1. a feed production technique for Bacillus licheniformis, is characterized in that being made up of following steps:
(1) Bacillus licheniformis is inoculated on substratum ferments;
(2) fermented liquid that step (1) obtains is carried out double-effect evaporation, obtain bacterium dope;
(3) the bacterium dope that step (2) obtains is sent in spray-dryer carries out spraying dry and obtain product.
2. a kind of feed as claimed in claim 1 production technique of Bacillus licheniformis, it is characterized in that the substratum composition described in step (1) is W-Gum 2-4%, dregs of beans 2-6%, peptone 1-3%, magnesium sulfate 1%, manganous sulfate 0.04%, potassium primary phosphate 1%, ferrous sulfate 0.01-0.05%, calcium carbonate 0.6% by mass percentage, all the other are soft water.
3. a kind of feed as claimed in claim 1 production technique of Bacillus licheniformis, it is characterized in that the inoculum size of Bacillus licheniformis be substratum quality 1%, leavening temperature 36 DEG C-37 DEG C, fermentation tank pressure 0.01-0.03MPa, fermentation air flow 1: 1, fermentation mixing speed: 150 revs/min, ph value 6-8.
4. a kind of feed as claimed in claim 1 production technique of Bacillus licheniformis, is characterized in that the double-effect evaporation in described step (2), and the temperature of evaporator room is 65-75 DEG C, vacuum tightness is-0.05 MPa ~-0.065MPa.
5. a kind of feed as claimed in claim 1 production technique of Bacillus licheniformis, it is characterized in that the spray-dryer in described step (3), inlet temperature in spray-dryer tower 150 DEG C-160 DEG C, temperature 80 DEG C-90 DEG C in tower, vacuum tightness-0.01 MPa ~-0.03 MPa, air outlet temperature 70 DEG C-80 DEG C.
6. a kind of feed as claimed in claim 1 production technique of Bacillus licheniformis, is characterized in that in described step (2), and the solid content obtaining bacterium dope after double-effect evaporation is 15%-25%.
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| CN201410480613.XA CN104293700A (en) | 2014-09-19 | 2014-09-19 | Production process of bacillus licheniformis for feed |
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| CN201410480613.XA CN104293700A (en) | 2014-09-19 | 2014-09-19 | Production process of bacillus licheniformis for feed |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105062925A (en) * | 2015-08-25 | 2015-11-18 | 中农颖泰林州生物科园有限公司 | Water-soluble bacillus licheniformis culture medium for feed and protection technology |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1382213A (en) * | 1999-10-01 | 2002-11-27 | 诺沃奇梅兹有限公司 | Spray dried enzyme product |
| CN101724560A (en) * | 2009-11-23 | 2010-06-09 | 山东盛隆生物工程有限公司 | Microbial inoculums and preparation method thereof |
| CN101880699A (en) * | 2010-06-29 | 2010-11-10 | 尤越 | Method for producing chitooligosaccharides by using microbial fermentation |
| CN103444985A (en) * | 2013-09-23 | 2013-12-18 | 西北农林科技大学 | Feed additive and manufacturing method and application thereof |
-
2014
- 2014-09-19 CN CN201410480613.XA patent/CN104293700A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1382213A (en) * | 1999-10-01 | 2002-11-27 | 诺沃奇梅兹有限公司 | Spray dried enzyme product |
| CN101724560A (en) * | 2009-11-23 | 2010-06-09 | 山东盛隆生物工程有限公司 | Microbial inoculums and preparation method thereof |
| CN101880699A (en) * | 2010-06-29 | 2010-11-10 | 尤越 | Method for producing chitooligosaccharides by using microbial fermentation |
| CN103444985A (en) * | 2013-09-23 | 2013-12-18 | 西北农林科技大学 | Feed additive and manufacturing method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 孟祥坤 等: "拮抗细菌枯草芽孢杆菌T429喷雾干燥工艺研究", 《中国生物防治学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105062925A (en) * | 2015-08-25 | 2015-11-18 | 中农颖泰林州生物科园有限公司 | Water-soluble bacillus licheniformis culture medium for feed and protection technology |
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