CN104292878B - Method for preparing rhodotorula benthica microcapsule pigment - Google Patents
Method for preparing rhodotorula benthica microcapsule pigment Download PDFInfo
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- CN104292878B CN104292878B CN201410536241.8A CN201410536241A CN104292878B CN 104292878 B CN104292878 B CN 104292878B CN 201410536241 A CN201410536241 A CN 201410536241A CN 104292878 B CN104292878 B CN 104292878B
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- ocean rhodotorula
- pigment
- rhodotorula
- ocean
- microcapsules pigment
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B67/00—Influencing the physical, e.g. the dyeing or printing properties of dyestuffs without chemical reactions, e.g. by treating with solvents grinding or grinding assistants, coating of pigments or dyes; Process features in the making of dyestuff preparations; Dyestuff preparations of a special physical nature, e.g. tablets, films
- C09B67/0097—Dye preparations of special physical nature; Tablets, films, extrusion, microcapsules, sheets, pads, bags with dyes
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- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Manufacturing Of Micro-Capsules (AREA)
Abstract
The invention discloses a method for preparing a rhodotorula benthica microcapsule pigment. Rhodotorula benthica suspension is broken by adopting a high-pressure micro jet homogenizer under the condition that the temperature is 4-10 DEG C, the pressure is 80-100MPa and the treatment rate is 0.08-0.10min/L. According to the method, rhodotorula benthica cells are homogenized under high pressure by adopting the high-pressure micro jet breaking technology, so that the rhodotorula benthica cells are subjected to mechanical force actions of high-speed shearing, high-frequency oscillation, hole effect, convection impact and the like and micro-broken to obtain the rhodotorula benthica microcapsule pigment; and in the preservation process of the product, the pigment is slowly released through micropores, so that the product can be stably colored for long time. The microcapsule pigment, which is prepared by using rhodotorula benthica as a raw material and micro-breaking the cells of the rhodotorula benthica through the high-pressure micro jet breaking technology, is low in cost, good in stability and better in coloring effect.
Description
Technical field
The invention belongs to pigment technical field, more particularly, to a kind of preparation method of ocean rhodotorula microcapsules pigment.
Background technology
With the further investigation to synthetic food color harmfulness, synthetic dyestuff will have, in future, the possibility comprehensively being disabled,
And pigment consumption in the world's, just towards developing in the direction of " natural, healthy, safety ", applies natural colouring matter will become following colorant markets
Main flow and main trend.Research shows, many natural colouring matters are physiological activators, has nutrition, prevention from suffering from the diseases and treatment and makees
With；Because of its Nantural non-toxic, have has health value again, and consumer favours accept, its promise well.Natural colouring matter main source
In plant, animal and microorganism three mcroorganism material, the wherein overwhelming majority is extracted from plant, but is limited and processing conditions by raw material
Impact, expensive, property is often unstable；Obtain from microorganism or prepare natural colouring matter then relatively economical, represent following sending out
Exhibition trend.
Capsicum red pigment is a kind of relatively common natural food pigment, is such as usually used in chicken conditioning food and is combined preserved materials
In, but it is easily affected by illumination and temperature, and especially light resistance is poor, color less stable, fugitive color.Research shows, exposes
Capsicum red pigment in the sunlight easily loses, and the product adding capsicum red pigment also can substantially fade, and therefore need to be situated between in oiliness
Keep in Dark Place in matter；Temperature is higher, and capsicum red pigment loss is also more, become apparent from more than 70 DEG C (king practices. capsicum red pigment
Extract [D]. Chongqing：Chongqing Sanxia Institute, 2011.).Additionally, capsicum red pigment not only first also will will remove different through extracting
Taste, operation is more numerous and diverse and relatively costly；The strong base substance such as NaOH can be used when eliminating the unusual smell, have certain safety hidden
Suffer from；Meanwhile, capsicum red pigment per se with pungent also limit its application.For all the foregoing reasons, some illegal retailers
Illegal using cheap more stable synthetic food color tonyred in food, cause huge " the Sudan's happy occasion of impact
The food-safety problems such as part ", but this is strict disabling.Therefore, find a kind of new natural colouring matter red to substitute capsicum
Application in food for the element has important practical significance.
High pressure microjet crushing technology is exactly to act on processed thing by carrying the high pressure microjet of huge energy
On material, and produce pressure transient in material inside and outside, so that crushing material.This crushing process moment can complete, and heats up limited,
The bioactive ingredients of powder therefore for heat sensitive material, can be retained to greatest extent, be beneficial to needed for making
High-quality product；The material particular diameter distribution dispersion pulverized through high pressure microjet is little, largely increased the ratio of micro mist
Surface area, makes adsorptivity, dissolubility accordingly increase；High pressure microjet crushing technology is applied to breaking-wall cell, cell segmentation etc.
Aspect also has significant emulsifying effect.High pressure microjet crushing technology can also accomplish that other equipment is unapproachable super
Finely divided degree and quality, or even play the effect of nano-dispersed.This make this technology such as clasmatosis, microcapsules prepare,
The fields such as microemulsified suffer from good application prospect.
Content of the invention
The purpose of the embodiment of the present invention is to provide a kind of preparation method of ocean rhodotorula microcapsules pigment it is intended to solve
Unstable present in existing natural colouring matter capsicum red pigment processing and storage process, fugitive color, there is the peculiar smell such as pungent, high cost
Problem.
The embodiment of the present invention is achieved in that a kind of preparation method of ocean rhodotorula microcapsules pigment, and this ocean is red
The preparation method of yeast microcapsules pigment adopts M-700 type high pressure Microfluidizer pulverization process ocean rhodotorula bacteria suspension 1
Secondary；High pressure Microfluidizer treatment conditions are：4 DEG C～10 DEG C, 80MPa～100MPa, 0.08min/L～0.10min/L.
Further, need red for ocean ferment before using high pressure Microfluidizer pulverization process ocean rhodotorula bacteria suspension
Female dry powder is added to the bacteria suspension being made into 10g/L～15g/L in distilled water.
Further, by red for ocean ferment after using high pressure Microfluidizer pulverization process ocean rhodotorula bacteria suspension 1 time
Female bacteria suspension filters, abandoning supernatant, obtains ocean rhodotorula microcapsules pigment wet-milling.
Further, after obtaining ocean rhodotorula microcapsules pigment wet-milling, wet-milling is put into lyophilized in freeze drying box, that is, make
Obtain ocean rhodotorula microcapsules pigment, vacuum packaging, storage are standby.
Further, equivalent substitution capsicum red pigment when ocean rhodotorula microcapsules pigment adds in food.
Further, the preparation method of this ocean rhodotorula microcapsules pigment comprises the following steps：
Step one, the microorganism fungus kind for preparing natural colouring matter is ocean rhodotorula；
Step 2, ocean rhodotorula bacterium dry powder is added to the bacteria suspension being made into 10g/L～15g/L in distilled water；
Step 3, using high pressure Microfluidizer at 4 DEG C～10 DEG C, 80MPa～100MPa, 0.08min/L～
Process ocean rhodotorula bacteria suspension 1 time under the conditions of 0.10min/L；
Step 4, the bacteria suspension after processing is filtered, abandoning supernatant, obtains ocean rhodotorula microcapsules pigment wet-milling；
Step 5, wet-milling is put into lyophilized in vacuum freezing drying oven, that is, ocean rhodotorula microcapsules pigment, vacuum is obtained
Packaging, storage are standby.
The preparation method of the ocean rhodotorula microcapsules pigment that the present invention provides, by high pressure microjet crushing technology to sea
Fuchsin yeast cells carries out high-pressure homogeneous, makes the mechanical masterpiece such as to be clashed into by high speed shear, the higher-order of oscillation, void effect and convection current
With reaching micro- broken and ocean rhodotorula microcapsules pigment being obtained；In product storage, it is slow that pigment will also pass through micropore
Discharge, so that product can colour long-term and stably.The present invention is with ocean rhodotorula as raw material, micro- with high pressure
Jet mill technology carries out micro- break process to its cell, is prepared for a kind of low cost, good stability, coloring effect more preferably micro-
Capsule pigment.
Brief description
Fig. 1 is the preparation method flow chart of ocean rhodotorula microcapsules pigment provided in an embodiment of the present invention；
Fig. 2 is capsicum red pigment spectrogram provided in an embodiment of the present invention, in figure：Abscissa represents wavelength, ordinate table
Show absorbance；
Fig. 3 is ocean rhodotorula microcapsules pigment spectrogram provided in an embodiment of the present invention, in figure：Abscissa represents ripple
Long, ordinate represents absorbance.
Specific embodiment
In order that the objects, technical solutions and advantages of the present invention become more apparent, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not used to
Limit the present invention.
Below in conjunction with the accompanying drawings and specific embodiment is further described to the application principle of the present invention.
As shown in figure 1, the preparation method of the ocean rhodotorula microcapsules pigment of the embodiment of the present invention comprises the following steps：
S101：Ocean rhodotorula dry powder is added to the bacteria suspension being made into 10g/L～15g/L in distilled water；
S102：Using high pressure Microfluidizer pulverization process bacteria suspension 1 time；
S103：Bacteria suspension after processing is filtered, abandoning supernatant, obtain ocean rhodotorula microcapsules pigment wet-milling；
S104：Wet-milling is put into lyophilized in freeze drying box, that is, ocean rhodotorula microcapsules pigment is obtained, be vacuum-packed,
Storage is standby.
Limit further be high pressure Microfluidizer treatment conditions as：4 DEG C～10 DEG C, 80MPa～100MPa,
0.08min/L～0.10min/L.
Limit further when being that obtained ocean rhodotorula microcapsules pigment adds in food can equivalent substitution peppery
Green pepper haematochrome.
The operation principle of the present invention：
Ocean rhodotorula microcapsules pigment belongs to natural colouring matter, and its tone and capsicum red pigment are very close to, and is present in and is in
In the micro- smudge cells of cell membrane of microcapsules state, cell wall has the micropore of adsorptivity, has protective effect to pigment, can
Stop its colour fading；Simultaneously in product storage, pigment will also pass through micropore and lentamente discharges, so that product can
Colour long-term and stably.
The specific embodiment of the present invention：
Embodiment 1：
Step one, the microorganism fungus kind for preparing natural colouring matter is ocean rhodotorula；
Step 2, ocean rhodotorula bacterium dry powder is added to the bacteria suspension being made into 12g/L in distilled water；
Step 3, using high pressure Microfluidizer at 8 DEG C, process bacteria suspension 1 time under the conditions of 90MPa, 0.09min/L；
Step 4, the bacteria suspension after processing is filtered, abandoning supernatant, obtains ocean rhodotorula microcapsules pigment wet-milling；
Step 5, wet-milling is put into lyophilized in vacuum freezing drying oven, that is, ocean rhodotorula microcapsules pigment, vacuum is obtained
Packaging, storage are standby.
Do in conjunction with the property of the ocean rhodotorula microcapsules pigment to the present invention for the following experiment, stability and application effect
Further instruction：
First, weigh the ocean rhodotorula microcapsules pigment 1g obtained by capsicum red pigment and the present invention respectively, be dissolved in 20mL
In acetone, soak 2h, fully vibrate and filter, make the acetone soln of pigment.With Shimadzu VU-2501PC in 250～650nm model
Enclose whole scanning and obtain spectrogram such as Fig. 2, Fig. 3 it can be seen that under same solvent, same diluted concentration, two kinds of pigments are scanned
Gained spectral line characteristic is essentially identical, therefore may certify that ocean rhodotorula microcapsules pigment can equivalent substitution capsicum red pigment.
2nd, respectively 50g capsicum red pigment is placed in plate with ocean rhodotorula microcapsules pigment and shakeouts into same area
Circle, be re-exposed to 20 DEG C, under 400lux fluorescent lamp intensity of illumination, measure its a every 4d respectively with CR-400 colour difference meter*
Value, is repeated 3 times.Storage-stable result such as table 1.
Table 1 capsicum red pigment and ocean rhodotorula microcapsules pigment stability
Note：a*Change reflection pigment stability, change is bigger, more unstable.Following table is same.
3rd, further illustrate with reference to chicken meat sausage processing instance：By the way of mixing is cut and mixed, equivalent (0.5%) is added respectively
Capsicum red pigment and ocean rhodotorula microcapsules pigment in chicken meat sausage, two groups of chicken meat sausage products cut into slices respectively be placed in 0～4 DEG C,
Under 400lux fluorescent lamp intensity of illumination, measure its a every 4d respectively with CR-400 colour difference meter*Value, is repeated 3 times.Storage-stable
Result such as table 2.
Table 2 adds the chicken meat sausage color and luster storage-stable of capsicum red pigment and ocean rhodotorula microcapsules pigment
Be can be seen that by Tables 1 and 2, ocean rhodotorula microcapsules pigment has more preferable stability.Advantages of the present invention exists
In having selected ocean rhodotorula bacterial classification, it can be obtained in a large number by fermented and cultured, and cost is relatively low.Preparation side of the present invention
Method is easily controllable, easy and simple to handle, and gained microcapsules pigment production is high, stability is big.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all essences in the present invention
Any modification, equivalent and improvement made within god and principle etc., should be included within the scope of the present invention.
Claims (6)
1. a kind of preparation method of ocean rhodotorula microcapsules pigment is it is characterised in that this ocean rhodotorula microcapsules pigment
Preparation method is first ocean rhodotorula bacterium dry powder to be added in distilled water to be made into ocean rhodotorula bacteria suspension, then adopts M-
700 type high pressure Microfluidizer pulverization process ocean rhodotorula bacteria suspensions 1 time；High pressure Microfluidizer treatment conditions are：4
DEG C～10 DEG C, 80MPa～100MPa, 0.08min/L～0.10min/L.
2. the preparation method of ocean rhodotorula microcapsules pigment as claimed in claim 1 is it is characterised in that micro- using high pressure
Need ocean rhodotorula dry powder is added in distilled water before jet homogenizer pulverization process ocean rhodotorula bacteria suspension and be made into
The bacteria suspension of 10g/L～15g/L.
3. the preparation method of ocean rhodotorula microcapsules pigment as claimed in claim 1 is it is characterised in that micro- using high pressure
After jet homogenizer pulverization process ocean rhodotorula bacteria suspension 1 time, ocean rhodotorula bacteria suspension is filtered, abandoning supernatant, obtain
Ocean rhodotorula microcapsules pigment wet-milling.
4. the preparation method of ocean rhodotorula microcapsules pigment as claimed in claim 3 is it is characterised in that obtain the red ferment in ocean
After female microcapsules pigment wet-milling, wet-milling is put into lyophilized in freeze drying box, that is, ocean rhodotorula microcapsules pigment, vacuum are obtained
Packaging, storage are standby.
5. the preparation method of ocean rhodotorula microcapsules pigment as claimed in claim 4 is it is characterised in that ocean rhodotorula is micro-
Equivalent substitution capsicum red pigment when capsule pigment adds in food.
6. the preparation method of ocean rhodotorula microcapsules pigment as claimed in claim 1 is it is characterised in that this ocean rhodotorula
The preparation method of microcapsules pigment comprises the following steps：
Step one, the microorganism fungus kind for preparing microcapsules pigment is ocean rhodotorula；
Step 2, ocean rhodotorula bacterium dry powder is added to and is made into the ocean rhodotorula bacterium of 10g/L～15g/L in distilled water and hangs
Liquid；
Step 3, using high pressure Microfluidizer at 4 DEG C～10 DEG C, 80MPa～100MPa, 0.08min/L～0.10min/L
Under the conditions of process ocean rhodotorula bacteria suspension 1 time；
Step 4, the ocean rhodotorula bacteria suspension after processing is filtered, abandoning supernatant, obtains ocean rhodotorula microcapsules pigment
Wet-milling；
Step 5, wet-milling is put into lyophilized in vacuum freezing drying oven, that is, ocean rhodotorula microcapsules pigment, vacuum packet is obtained
Dress, storage are standby.
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| CN201410536241.8A CN104292878B (en) | 2014-10-11 | 2014-10-11 | Method for preparing rhodotorula benthica microcapsule pigment |
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| CN201410536241.8A CN104292878B (en) | 2014-10-11 | 2014-10-11 | Method for preparing rhodotorula benthica microcapsule pigment |
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| CN104292878B true CN104292878B (en) | 2017-02-08 |
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| CN105295439B (en) * | 2015-12-02 | 2017-06-16 | 武汉绿孚生物工程有限责任公司 | A kind of preparation method of microencapsulation Gardenia Yellow |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101148643A (en) * | 2006-09-22 | 2008-03-26 | 中国科学院沈阳应用生态研究所 | Producing method of rhodotorula mucilaginosa for holothurian |
| CN101705193A (en) * | 2009-11-20 | 2010-05-12 | 中国水产科学研究院黄海水产研究所 | Astaxanthin-producing ocean rhodotorula YS-185 and method for producing astaxanthin thereof |
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2014
- 2014-10-11 CN CN201410536241.8A patent/CN104292878B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101148643A (en) * | 2006-09-22 | 2008-03-26 | 中国科学院沈阳应用生态研究所 | Producing method of rhodotorula mucilaginosa for holothurian |
| CN101705193A (en) * | 2009-11-20 | 2010-05-12 | 中国水产科学研究院黄海水产研究所 | Astaxanthin-producing ocean rhodotorula YS-185 and method for producing astaxanthin thereof |
Non-Patent Citations (1)
| Title |
|---|
| 红酵母中β-胡萝卜素提取工艺研究;刘洋等;《现代畜牧兽医》;20131231;55-60 * |
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