CN104285544B - A kind of tiny Seed sterilization method of orchid and steriliser - Google Patents
A kind of tiny Seed sterilization method of orchid and steriliser Download PDFInfo
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- CN104285544B CN104285544B CN201410621780.1A CN201410621780A CN104285544B CN 104285544 B CN104285544 B CN 104285544B CN 201410621780 A CN201410621780 A CN 201410621780A CN 104285544 B CN104285544 B CN 104285544B
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- steriliser
- seed
- steel wire
- lid
- orchid
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Abstract
nullThe invention discloses a kind of tiny Seed sterilization method of orchid and steriliser，The present invention by arrange steriliser and relative to sterile solution，The solution concentration of employing science and sterilization time，Make when sterilizing，Seed energy full and uniform contact with sterile solution (sterile solution can flow in or out steriliser by the grid in steel wire uniformly)，Simultaneously in the later stage cultivates，Have only to change steel wire on lid (diminish by the order number of steel wire，Become large-sized)，Then seed can be flowed in culture medium uniformly through steel wire，Complete sterilizing cultivation，The present invention makes seed can contact with biocide uniformly in sterilization process，Improve the time of contact of seed and biocide，Sterilization process can effectively be held sterilization time，Seed for cracking can make full use of，The seed not having cracking can effectively improve the germination period of seed，Cost of manufacture of the present invention is cheap simultaneously、Easy to operate、Make simple、Can reuse.
Description
Technical field
The present invention relates to a kind of tiny Seed sterilization method of orchid and steriliser, belong to orchid tissue sterilization technology field.
Background technology
The orchid family (Orchidaceae) plant is widely used in the fields such as Chinese crude drug, flowers, greening.Usual Orchid Seeds is very tiny, such as Pseudobulbus Bletillae (Rhizoma Bletillae), Herba Dendrobii, Cremastra appendiculata, Rhizoma Gastrodiae etc., its shape is just high-visible under the microscope, most Orchid Seeds do not have endosperm, only several do not have full grown embryo, cause that the such as seed such as Pseudobulbus Bletillae (Rhizoma Bletillae), Herba Dendrobii, Cremastra appendiculata is difficult to sprout under natural environment.Aseptically, adopting tissue culture to carry out artificial sexual propagation is one of numerous soon effective means of most orchid, and in tissue culture procedures, the seed asepsis of the aseptic process of seed especially dehiscent capsule is treated as one of key problem in technology；Aseptic process to tiny cracking seed such as such as Pseudobulbus Bletillae (Rhizoma Bletillae), Herba Dendrobii etc., it is common to use be that gauze parcel seed processes, but arise that sterilizing is uneven, insufficient in this process, adhere to, the phenomenon such as difficult sowing, easy pollution, cause the waste of resource.
Summary of the invention
It is an object of the invention to: the defect existed for prior art, a kind of tiny Seed sterilization method of orchid and steriliser are provided, make seed can contact with biocide uniformly in sterilization process, improve the time of contact of seed and biocide, to overcome the deficiencies in the prior art.
Technical scheme
A kind of tiny Seed sterilization method of orchid, the method comprises the following steps:
Step one, steriliser is placed in super-clean bench, the seed of the orchid of cracking is put into steriliser and covers lid, will be equipped with the steriliser of the seed of orchid and put into and ooze in spirituous beaker, in beaker, then shake steriliser；
Step 2, the steriliser taken out from beaker place in mercury solution, then shake steriliser in beaker, are finally putting in sterilized water and rinse；
Step 3, employing drying machine drying seed, with the steel wire changed on lid, be then inverted steriliser, and concussion steriliser is by even distribution of seed sowing culture medium in culture bottle in steriliser.
In the tiny Seed sterilization method of aforesaid orchid, described steriliser is up big and down small cone structure, is provided with collar in steriliser, and steel wire is placed on bottom the steel wire and steriliser that on collar are a gap.
In the tiny Seed sterilization method of aforesaid orchid, described lid internal diameter is consistent with steriliser top outer diameter, lays removably steel wire in the middle part of lid.
In the tiny Seed sterilization method of aforesaid orchid, in described beaker, the concentration of ethanol is 60%-80%, and in beaker, the time of vibrations is 10-30s.
In the tiny Seed sterilization method of aforesaid orchid, the concentration of described hydrargyrum is 0.05%-0.15%, and in mercury solution, the resting period is 5-15min.
The steriliser of the tiny seed of a kind of orchid, including box body, arranges collar bottom box body, steel wire one be placed on collar and with box body bottom form gap, in the middle part of lid, be provided with removably steel wire two, box body and lid with the use of.
In the steriliser of the aforesaid tiny seed of a kind of orchid, described collar is hollow thin wall cylindrical structure, this structure makes to separate bottom steel wire one and box body, solution in vibrations process can fully repeatedly be flowed between seed, and seed itself will not stick to bottom box body, have only to clamp steel wire and replaceable with tweezers for the moment at replacing steel wire, convenient, safety simultaneously.
In the steriliser of the aforesaid tiny seed of a kind of orchid, described box body is up big and down small cone structure, facilitates the replacing of steel wire one by arranging this structure, make simultaneously steel wire one after being placed on collar place can with box body inside closely sturdy.
In the steriliser of the aforesaid tiny seed of a kind of orchid, lid internal diameter is consistent with box body top outer diameter, it is symmetrical arranged L-type groove in box body top outer, boss it is symmetrical arranged inside lid, boss is corresponding with L-type groove location and cooperates, turning-bar it is symmetrical arranged outside lid, when box body coordinates with lid, boss snaps in L-type groove until arriving L-type trench bottom, lid is rotated again through turning-bar, then boss snaps in the other end of L-type bottom land groove, thus completing the stuck of box body and lid, lid internal diameter is consistent with box body top outer diameter simultaneously, so that it is guaranteed that sealing.
In the steriliser of the aforesaid tiny seed of a kind of orchid, handle is set in the middle part of box body, by arranging handle so that operator can realize shaking in alcoholic solution and mercury solution by turning handle, and the hands of operator need not contact with above-mentioned solution.
nullOwing to have employed technique scheme，Compared with prior art，The present invention is by arranging steriliser and corresponding sterile solution，The solution concentration of employing science and sterilization time，Make when sterilizing，Seed energy full and uniform contact with sterile solution (sterile solution can flow in or out steriliser by the grid in steel wire uniformly)，Simultaneously in the later stage cultivates，Have only to change steel wire on lid (diminish by the order number of steel wire，Become large-sized)，Then seed can be flowed in culture medium uniformly through steel wire，Complete sterilizing cultivation，The present invention makes seed can contact with biocide uniformly in sterilization process，Improve the time of contact of seed and biocide，Sterilization process can effectively be held sterilization time，Seed for cracking can make full use of，The seed not having cracking can effectively improve the germination period of seed，Cost of manufacture of the present invention is cheap simultaneously、Easy to operate、Make simple、Can reuse.
Accompanying drawing explanation
Accompanying drawing 1 is the structural representation of steriliser in the present invention；
Accompanying drawing 2 is the structural representation of the box body in the present invention equipped with steel wire one；
Accompanying drawing 3 is the structural representation of the box body not having steel loading silk screen one in the present invention；
Accompanying drawing 4 is the structural representation of lid in the present invention.
Detailed description of the invention
Below in conjunction with accompanying drawing to the present invention with being described in further detail, but not as any limitation of the invention.
Embodiments of the invention: a kind of tiny Seed sterilization method of orchid, the method comprises the following steps:
Step one, steriliser first install each parts before use and namely steel wire are put into the upper fixing steel wire one of circular collar of sth. made by twisting bottom box body, the steel wire two of identical order number is put into lid, cover lid and twist with the fingers tight, steriliser and the steel wire of interchangeable order number is wrapped again with newspaper or kraft paper, put into high-pressure sterilizing pot sterilization, put in super-clean bench after bacterium of having gone out
Step 2, steriliser is placed in super-clean bench, the seed of the orchid of cracking is put into steriliser and covers lid, will be equipped with the steriliser of the seed of orchid and put in the beaker being impregnated with 75% ethanol, in beaker, then shake steriliser, take out after ten seconds；
Step 3, the steriliser taken out from beaker place in 0.1% mercury solution, then shake steriliser in beaker, take out, be finally putting in sterilized water and rinse after 10min；
Step 4, employing drying machine drying seed, with the steel wire changed on lid, (the order number of this steel wire is inconsistent with the order number of steel wire two, and size can pass through with seed), then steriliser to be inverted, even distribution of seed in steriliser is sowed in culture medium in culture bottle by concussion steriliser；
Accelerate dry if step 5 can also increase a hair-dryer with flexible pipe slowly in dry run.
Observing after having inoculated two weeks and just can find seed-bearing sprouting, the cracking seed that goes out with tradition compares, and has obvious advantage to pollute little, easy to operate.Sprout time substantially shortens compared with sterilizing capsule.
nullSteriliser according to the tiny seed of a kind of orchid needed for said method，As shown in drawings，Including box body 1，This box body 1 is up big and down small cone structure，The height of concrete box body 1 is 6cm，Bottom box body 1, external diameter is 4.5cm，Internal diameter is 4.0cm，Collar 2 is set bottom box body 1，This collar 2 is hollow thin wall cylindrical structure，Collar 2 is highly 0.5cm，Collar 2 diameter is 3.9cm，Box body 1 internal diameter is 4.25cm herein，Steel wire 1 be placed on collar 2 and with box body 1 bottom formed gap，Steel wire 1 is 200 orders and diameter is 4.25cm，Removably steel wire 25 it are provided with in the middle part of lid 4，Lid 4 external diameter is 5.05cm，Internal diameter is 4.5cm，Steel wire 25 is 200 orders and diameter is 4.45cm，Box body 1 and lid 4 with the use of.
Wherein it is symmetrical arranged L-type groove 6 in box body 1 top outer, L-type groove 6 vertically height 0.75cm, transversal length 0.5cm, groove width 0.3cm, boss 7 it is symmetrical arranged inside lid 4, this boss 7 length 0.25cm, diameter 0.3cm, boss 7 is corresponding with L-type groove 6 position and cooperates, outside lid 4, it is symmetrical arranged turning-bar 8, handle 9 is set in the middle part of box body 1.
Claims (6)
1. the tiny Seed sterilization method of orchid, it is characterised in that: the method comprises the following steps:
Step one, steriliser is placed in super-clean bench, the seed of the orchid of cracking is put into steriliser and covers lid, will be equipped with the steriliser of the seed of orchid to put into and ooze in spirituous beaker, then in beaker, shake steriliser, wherein this steriliser is up big and down small cone structure, collar it is provided with in steriliser, steel wire is placed on collar and makes there is a gap bottom steel wire and steriliser, wherein in beaker, the concentration of ethanol is 60%-80%, and in beaker, the time of vibrations is 10-30s；
Step 2, the steriliser taken out from beaker place in mercury solution, and the concentration of this hydrargyrum is 0.05%-0.15%, and in mercury solution, the resting period is 5-15min, then shake steriliser in beaker, are finally putting in sterilized water and rinse；
Step 3, employing drying machine drying seed, change the steel wire on lid, be then inverted by steriliser, and concussion steriliser is by even distribution of seed sowing culture medium in culture bottle in steriliser.
2. the tiny Seed sterilization method of orchid according to claim 1, it is characterised in that: described lid internal diameter is consistent with steriliser top outer diameter, lays removably steel wire in the middle part of lid.
3. the steriliser of the tiny seed of orchid, including box body (1), it is characterized in that this box body (1) is for up big and down small cone structure, in box body (1) bottom, collar (2) is set, steel wire one (3) is placed on collar (2) and above and forms gap with box body (1) bottom, be provided with removably steel wire two (5) at lid (4) middle part, box body (1) and lid (4) with the use of.
4. the steriliser of the tiny seed of a kind of orchid according to claim 3, it is characterised in that: described collar (2) is hollow thin wall cylindrical structure.
5. the steriliser of the tiny seed of a kind of orchid according to claim 3, it is characterized in that: lid (4) internal diameter is consistent with box body (1) top outer diameter, it is symmetrical arranged L-type groove (6) in box body (1) top outer, it is symmetrical arranged boss (7) in lid (4) inner side, boss (7) is corresponding with L-type groove (6) position and cooperates, and is symmetrical arranged turning-bar (8) in lid (4) outside.
6. the steriliser of the tiny seed of a kind of orchid according to claim 3, it is characterised in that: at box body (1) middle part, handle (9) is set.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410621780.1A CN104285544B (en) | 2014-11-07 | 2014-11-07 | A kind of tiny Seed sterilization method of orchid and steriliser |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410621780.1A CN104285544B (en) | 2014-11-07 | 2014-11-07 | A kind of tiny Seed sterilization method of orchid and steriliser |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104285544A CN104285544A (en) | 2015-01-21 |
| CN104285544B true CN104285544B (en) | 2016-07-06 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410621780.1A Expired - Fee Related CN104285544B (en) | 2014-11-07 | 2014-11-07 | A kind of tiny Seed sterilization method of orchid and steriliser |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106135007A (en) * | 2016-09-27 | 2016-11-23 | 中国科学院昆明植物研究所 | A kind of sterilizing being suitable to trace Orchid Seeds during in vitro conservation and inoculation method |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3951056B2 (en) * | 2004-03-31 | 2007-08-01 | タキイ種苗株式会社 | Granulated coated seed and method for producing the same |
| CN1257663C (en) * | 2004-05-24 | 2006-05-31 | 中国科学院东北地理与农业生态研究所 | Disinfection container in air permeability for vegetable seed and method of application |
| CN201905020U (en) * | 2010-11-02 | 2011-07-27 | 江苏省农业科学院 | Seed sterilizer based on chlorine disinfection |
| CN202444759U (en) * | 2012-03-08 | 2012-09-26 | 贵州省烟草科学研究所 | Jacketed flask of seed-accommodating pot |
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| CN104285544A (en) | 2015-01-21 |
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