CN104285544B - A kind of tiny Seed sterilization method of orchid and steriliser - Google Patents

Abstract

The invention discloses a method for sterilizing small orchid seeds and a sterilizer. The invention adopts a scientific solution concentration and a sterilizing time by setting the sterilizer and a relative sterilizing solution, so that when sterilizing, The seeds can fully and evenly contact with the sterilizing solution (the sterilizing solution can evenly flow into or out of the sterilizer through the mesh in the steel mesh), and at the same time in the later cultivation, only the steel mesh on the box cover needs to be replaced (that is, the steel mesh The number of mesh becomes smaller and the size becomes larger), then the seeds can evenly flow into the culture medium through the steel mesh to complete the sterilized cultivation. The contact time of the sterilizing agent can effectively grasp the sterilization time during the sterilization process, and can make full use of the cracked seeds, and can effectively improve the germination period of the seeds without cracking. At the same time, the invention has low production cost and is easy to operate. , Easy to make and can be used repeatedly.

Description

Method and sterilizer for sterilizing small orchid seeds

technical field

The invention relates to a method for sterilizing small orchid seeds and a sterilizer, belonging to the technical field of orchid tissue sterilization.

Background technique

Orchidaceae plants are widely used in traditional Chinese medicinal materials, flowers, greening and other fields. Usually the seeds of orchids are very small, such as Baiji, Dendrobium, Rhododendron, Gastrodia elata, etc., and their shapes can only be clearly seen under a microscope. Most orchids have no endosperm, only a few embryos that are not fully developed, resulting in such as white and , Dendrobium, Rhododendron and other seeds are difficult to germinate in the natural environment. Under sterile conditions, using tissue culture for artificial sexual reproduction is one of the effective means for the rapid propagation of most orchids, and in the process of tissue culture, the aseptic treatment of seeds, especially the aseptic treatment of dehiscated capsules, has become a key technology One; for the aseptic treatment of small cracked seeds such as white and dendrobium, usually use gauze to wrap the seeds for treatment, but in this process, there will be uneven sterilization, insufficient, adhesion, difficult sowing, Easy to pollute and other phenomena, resulting in waste of resources.

Contents of the invention

The purpose of the present invention is: aiming at the defects in the prior art, provide a method for sterilizing tiny orchid seeds and a sterilizer, so that the seeds can be evenly contacted with the sterilizing agent during the sterilization process, and improve the relationship between seeds and sterilizers. The contact time of bacterial agent, to overcome the deficiency of prior art.

Technical scheme of the present invention

A method for sterilizing tiny orchid seeds, the method comprising the following steps:

Step 1. Put the sterilizer in the ultra-clean bench, put the cracked orchid seeds into the sterilizer and cover the box cover, put the sterilizer containing the orchid seeds into the sterilizer in a beaker with alcohol, then shake the sterilizer in the beaker;

Step 2. Put the sterilizer taken out from the beaker into the mercury solution, then vibrate the sterilizer in the beaker, and finally put it into sterile water to rinse;

Step 3: Use a dryer to dry the seeds, replace the wire mesh on the box cover, then turn the sterilizer upside down, and shake the sterilizer to sow the seeds in the sterilizer evenly on the culture medium in the culture bottle.

In the aforementioned method for sterilizing small orchid seeds, the sterilizer is a cone structure with a large top and a small bottom, and a collar is provided in the sterilizer, and the steel wire mesh is placed on the collar so that the steel mesh and the There is a gap at the bottom of the sterilizer.

In the aforementioned method for sterilizing small orchid seeds, the inner diameter of the lid is consistent with the outer diameter of the top of the sterilizer, and a detachable steel wire mesh is placed in the middle of the lid.

In the aforementioned method for sterilizing tiny orchid seeds, the concentration of alcohol in the beaker is 60%-80%, and the time of shaking in the beaker is 10-30s.

In the aforementioned method for sterilizing small orchid seeds, the mercury concentration is 0.05%-0.15%, and the storage time in the mercury solution is 5-15min.

A sterilizer for small orchid seeds, including a box body, a collar is set at the bottom of the box body, a steel wire mesh is placed on the collar and forms a gap with the bottom of the box body, and a detachable steel wire is arranged in the middle of the box cover Net two, the box body and the box cover are used together.

In the aforementioned sterilizer for small orchid seeds, the collar is a hollow thin-walled cylindrical structure, which separates the steel wire mesh from the bottom of the box body, so that the solution can be fully absorbed during the vibration process. The seeds flow repeatedly, and the seeds themselves will not stick to the bottom of the box. At the same time, when replacing the wire mesh, you only need to clamp the wire mesh with tweezers to replace it, which is convenient and safe.

In the aforesaid sterilizer for small orchid seeds, the box body has a large upper and lower small cone structure. By setting this structure, it is convenient to replace the steel wire mesh one, and at the same time, the steel wire mesh one is placed on the collar. After processing, it can be tightly attached to the inner side of the box body.

In the aforementioned sterilizer for small orchid seeds, the inner diameter of the box cover is consistent with the outer diameter of the top of the box body, L-shaped grooves are arranged symmetrically on the outside of the top of the box body, and bosses are symmetrically arranged on the inner side of the box cover. The positions of the grooves correspond to each other and cooperate with each other. A steering rod is symmetrically arranged on the outside of the box cover. When the box body and the box cover are matched, the boss snaps into the L-shaped groove until it reaches the bottom of the L-shaped groove, and then rotates the box cover through the steering rod. The boss snaps into the other end of the bottom groove of the L-shaped groove, so as to complete the locking of the box body and the box cover, and at the same time, the inner diameter of the box cover is consistent with the outer diameter of the top of the box body, so as to ensure the sealing.

In the aforementioned sterilizer for small orchid seeds, a handle is provided in the middle of the box body. By setting the handle, the operator can vibrate in the alcohol solution and the mercury solution by turning the handle, and the operator's hand does not move. Contact with the above solution is required.

Due to the adoption of the above technical scheme, compared with the prior art, the present invention adopts a scientific solution concentration and sterilization time by setting a sterilizer and a corresponding sterilization solution, so that during sterilization, the seeds can be fully and evenly separated from the sterilization solution. The sterilizing solution is in contact with each other (the sterilizing solution can evenly flow into or out of the sterilizer through the mesh in the steel mesh), and at the same time in the later cultivation, only the steel mesh on the box cover needs to be replaced (that is, the mesh number of the steel mesh becomes smaller , the size becomes larger), then the seeds can evenly flow into the culture medium through the steel wire mesh to complete the sterilized cultivation. time, the sterilization time can be effectively grasped in the sterilization process, the cracked seeds can be fully utilized, and the seeds without cracks can effectively improve the germination period of the seeds. At the same time, the invention has low production cost, convenient operation, simple production, and can reuse.

Description of drawings

Accompanying drawing 1 is the structural representation of sterilizer among the present invention;

Accompanying drawing 2 is the structural representation of the box body that steel wire mesh one is housed among the present invention;

Accompanying drawing 3 is the structural representation of the box body that steel wire mesh 1 is not installed among the present invention;

Accompanying drawing 4 is the structural representation of box cover among the present invention.

detailed description

The present invention will be described in further detail below in conjunction with the accompanying drawings, but not as any limitation to the present invention.

Embodiments of the present invention: a method for sterilizing tiny orchid seeds, the method may further comprise the steps:

Step 1. Install the parts of the sterilizer before use, that is, put the steel wire mesh into the bottom of the box body, twist the circular collar to fix the steel mesh 1, put the steel mesh 2 of the same mesh number into the box cover, and cover the box Twist the cover tightly, then wrap the sterilizer and steel wire mesh with interchangeable mesh with newspaper or kraft paper, put it in an autoclave for sterilization, and put it into the ultra-clean bench after the bacteria are sterilized

Step 2. Put the sterilizer in the ultra-clean bench, put the cracked orchid seeds into the sterilizer and cover the box cover, put the sterilizer containing the orchid seeds into the sterilizer In a beaker with 75% alcohol, then shake the sterilizer in the beaker and take it out after ten seconds;

Step 3. Put the sterilizer taken out from the beaker into 0.1% mercury solution, then shake the sterilizer in the beaker, take it out after 10 minutes, and finally put it into sterile water to rinse;

Step 4: Use a dryer to dry the seeds, replace the steel wire mesh on the box cover (the mesh number of the steel mesh is inconsistent with the mesh number of the second steel mesh, and the size is enough for the seeds to pass through), and then put the sterilizer Inverted, the vibration sterilizer sows the seeds in the sterilizer evenly on the culture medium in the culture bottle;

Step 5. If the drying process is slow, you can add a hair dryer with a hose to speed up the drying.

After two weeks of inoculation, the seeds can be found to germinate. Compared with traditional cracking seeds, it has obvious advantages of less pollution and convenient operation. Compared with sterilized capsules, the germination time was significantly shortened.

According to the sterilizer of the tiny seeds of a kind of orchid plant required by the above-mentioned method, as shown in the accompanying drawing, comprise box body 1, this box body 1 is the structure of upper big and lower small cone, the height of concrete box body 1 is 6cm, the outer diameter of the bottom of the box body 1 is 4.5cm, the inner diameter is 4.0cm, and the collar 2 is set at the bottom of the box body 1. The collar 2 is a hollow thin-walled cylindrical structure, the height of the collar 2 is 0.5cm, and the collar 2 The diameter is 3.9cm, and the inner diameter of the box body 1 is 4.25cm. The steel mesh 13 is placed on the collar 2 and forms a gap with the bottom of the box body 1. The steel mesh 13 is 200 mesh and the diameter is 4.25cm. 4 middle parts are provided with detachable steel wire mesh 2 5, and lid 4 external diameter is 5.05cm, and internal diameter is 4.5cm, and steel wire mesh 2 5 is 200 orders and diameter is 4.45cm, and box body 1 cooperates with lid 4 and uses.

Wherein the L-shaped groove 6 is arranged symmetrically on the outer side of the box body 1 top, the vertical height of the L-shaped groove 6 is 0.75cm, the cross-sectional length is 0.5cm, and the width of the groove is 0.3cm, and the boss 7 is symmetrically arranged on the inside of the lid 4, and the boss 7 Length 0.25cm, diameter 0.3cm, boss 7 and L-shaped groove 6 positions correspond and cooperate with each other, and steering rod 8 is arranged symmetrically outside box cover 4, and handle 9 is set at box body 1 middle part.

Claims (6)

1. a method for sterilizing tiny seeds of orchidaceae, is characterized in that: the method may further comprise the steps: Step 1. Put the sterilizer in the ultra-clean bench, put the cracked orchid seeds into the sterilizer and cover the box cover, put the sterilizer containing the orchid seeds into the sterilizer Put it in a beaker with alcohol, and then shake the sterilizer in the beaker. The sterilizer is a cone structure with a large upper part and a smaller lower part. There is a collar in the sterilizer, and the steel wire mesh is placed on the collar to make the steel mesh There is a gap with the bottom of the sterilizer, the concentration of alcohol in the beaker is 60%-80%, and the vibration time in the beaker is 10-30s; Step 2: Put the sterilizer taken out from the beaker into the mercury solution, the concentration of the mercury is 0.05%-0.15%, the storage time in the mercury solution is 5-15min, then shake the sterilizer in the beaker, and finally Rinse in sterile water; Step 3: Use a dryer to dry the seeds, replace the wire mesh on the box cover, then turn the sterilizer upside down, and vibrate the sterilizer to sow the seeds in the sterilizer evenly on the culture medium in the culture bottle. 2. The method for sterilizing small orchid seeds according to claim 1, characterized in that: the inner diameter of the lid is consistent with the outer diameter of the top of the sterilizer, and a detachable steel wire mesh is placed in the middle of the lid. 3. A sterilizer for small orchid seeds, including a box body (1), characterized in that the box body (1) is a small cone structure with an upper part and a lower part, and a collar ( 2), the steel wire mesh one (3) is placed on the collar (2) and forms a gap with the bottom of the box body (1), and there is a detachable steel wire mesh two (5) in the middle of the box cover (4), the box body ( 1) Used in conjunction with the box cover (4). 4. The sterilizer for tiny orchid seeds according to claim 3, characterized in that: the collar (2) is a hollow thin-walled cylindrical structure. 5. A sterilizer for small orchid seeds according to claim 3, characterized in that: the inner diameter of the box cover (4) is the same as the outer diameter of the top of the box body (1), and is outside the top of the box body (1). The L-shaped groove (6) is arranged symmetrically, and the boss (7) is symmetrically arranged on the inner side of the box cover (4). Set the steering rod (8). 6. A sterilizer for tiny orchid seeds according to claim 3, characterized in that: a handle (9) is provided in the middle of the box body (1).