CN104274354A - Mixture of lycium ruthenicum extract and black tomato extract and application of mixture - Google Patents
Mixture of lycium ruthenicum extract and black tomato extract and application of mixture Download PDFInfo
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- CN104274354A CN104274354A CN201310293689.7A CN201310293689A CN104274354A CN 104274354 A CN104274354 A CN 104274354A CN 201310293689 A CN201310293689 A CN 201310293689A CN 104274354 A CN104274354 A CN 104274354A
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Abstract
The invention provides a psammophyte mixture and an application of the psammophyte mixture. The psammophyte mixture is a mixture of a lycium ruthenicum extract and a black tomato extract. The mixture of the two extracts has good efficacies of whitening, moisturizing, screening the sun and resisting senility, and can be applied to the fields of cosmetics, health foods and medicines.
Description
Technical field
The invention belongs to field of natural product chemistry, particularly relate to mixture and the application thereof of a kind of black Fructus Lycii extract and black tomato extract.
Background technology
Severe drought, in the desert that natural conditions are very severe, also grows the wild plant of about 1000 kinds, comprising commercial timber, medicinal plants, fibre plant etc. that many economic worths are higher.Be exactly below several in psammophytes.
Black Fructus Lycii (Lycium ruthenicum Murr.) is distributed in high mountain sarin, salinization sand ground, lake, river bank, dry wash, Desert Riparian Forest, is China's western distinctive desert medicinal plants kind.Fragmentary distribution is all had on China's Helan Mountain in Ningxia, East of Qinghai Province, In The North of Xinjiang, West Inner Mongolia, North Shaanxi, Gansu and Tibet and other places.Its growth cannot survive the mankind waste hill bell, sandy beach, riverbed, have extremely strong vitality.Black Fructus Lycii sweet in the mouth, property are flat, and detect through science, its calcium, ferrum, nicotinic acid content are respectively 2.3,4.6,16.7 times of Fructus Pyracanthae Fructus Lycii, and especially procyanidin (OPC) exceedes blue berry (black Fructus Lycii is containing OPC3690mg/100g; Blue berry is containing OPC330 ~ 3380mg/100g).This is the wild fruit finding that OPC content is the highest up to now, and be also the most effective Natural antioxidant, its effect is V
c20 times, V
e50 times, there is superpower enhancing immunity, the tonic effect of slow down aging.
Black tomato (Solanum lycopersicum) originates in South America, is the treasure of Fructus Lycopersici esculenti family, gains the name because its fruit normal complexion fruit is reddish black.Its fruit dietotherapeutic, has strong fruit aroma, and the mouthfeel of moderately sour and sweet, is of high nutritive value, and is particularly suitable for eating raw, also containing a large amount of lycopene and abundant vitamin C and antioxidant, has YIN nourishing to protect kidney and weight-reducing effect to women.Britain " Guardian " reports, the achievement in research of Canadian Studies personnel under a tomato sauce company sponsors finds, containing than lycopene (Lycopene) more substantial in Fructus Lycopersici esculenti and antioxidant and vitamin C in black tomato, structure is more reasonable, more easily be absorbed by the body, there is Breast Cancer Prevention, cervical cancer, carcinoma of prostate, colon cancer and cardiopathic effect, again with the particular significant effect of Breast Cancer Prevention.
But, had not yet to see about psammophytes, especially black Fructus Lycii and the black tomato investigation and application in beauty and skin care.
Summary of the invention
The object of the invention is to: a kind of psammophytes mixture is provided, be intended to the activity extract in psammophytes to be applied in cosmetics, health product or medicine, be especially preferred in skin preparations for extenal use, to play the effect of beauty and skin care.
The object of the present invention is achieved like this:
A kind of psammophytes mixture, is specially the mixture of black Fructus Lycii extract and black tomato extract.
Wherein, in mixture, the weight ratio of black Fructus Lycii extract and black tomato extract is 1-10: 1-10, is preferably 1-10: 1-5.
Described black Fructus Lycii extract and black tomato extract pass through respectively black Fructus Lycii and black tomato raw material to be flooded organic solvent and carry out after extraction filtering, reducing pressure and concentrated rear acquisition, again these two kinds of extracts are mixed by a certain percentage, carry out the experimentation of anti-aging effects, whitening function, anti-sunlight function and moisture-keeping function respectively.
Described organic solvent refers to the organic solvent containing hydroxyl or carbonyl isopolarity group, such as: water, Methanamide, dimethyl formamide, hexamethyl phosphoramide, tetramethylethylenediamine, triethylamine, tri-n-butylamine, trioctylamine, acetic acid, trifluoroacetic acid, acetonitrile, methyl formate, ethyl acetate, DMC dimethyl carbonate, dimethyl sulfoxine, acetone, butanone, dioxane, pyridine, oxolane, methanol, ethanol, acetate fiber, chloroform, glycerol, propylene glycol, propylene glycol, isopropyl alcohol, n-butyl alcohol and ether etc., in one or more, wherein preferred alcohol aqueous solution.
When taking ethanol water as organic solvent, the concentration of ethanol selects 30-90%, preferred 50-90%.
Known according to test, the mixture of black Fructus Lycii extract and black tomato extract all has good effect in whitening, moisturizing, sun-proof, defying age.
The mixture of black Fructus Lycii extract and black tomato extract can be applicable to skin preparations for extenal use, health food and medicine, especially can be applicable in cosmetics, the consumption different according to the dissimilar interpolation of preparation.
Accompanying drawing explanation
Fig. 1 is the uv absorption test pattern of the mixture (10: 1) of the black Fructus Lycii extract that provides of the embodiment of the present invention and black tomato extract.
Detailed description of the invention
In order to make object of the present invention, technical scheme and advantage clearly understand, below in conjunction with drawings and Examples, the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
Embodiment 1: the preparation of psammophytes extract
Powder 50g after lycium ruthenicum fruit drying and crushing, with 70% ethanol 500ml merceration, supersound extraction 2 times under room temperature, each 30min (500W), filtrate merges, and decompression and solvent recovery, to dry, obtains black Fructus Lycii extract.
Powder 50g after black tomato fruit drying and crushing, with 70% ethanol 500ml merceration, supersound extraction 2 times under room temperature, each 30min (500W), filtrate merges, and decompression and solvent recovery, to dry, obtains black tomato extract.
Get black Fructus Lycii extract and black tomato extract, carry out proportioning mixing according to part by weight 10: 1,5: 1,1: 1,1: 5 and 1: 10 respectively, be mixed with solution, for corresponding efficacy test.
Embodiment 2: the anti-aging effects research of psammophytes extract combination
1), free radical scavenging Effect study
Principle: have single electron according to DPPH free radical, the last one is had to absorb at 517nm place, its alcoholic solution is the characteristic of purple, when there being free radical scavenger to exist, make it absorb owing to matching with its single electron to fade away, its fading extent becomes quantitative relationship with the electron amount of its acceptance, thus can carry out quantitative study fast with spectrophotometer, detect free radical scavenging situation, thus whether judgement sample has oxidation resistance.
Test method: the testing sample solution 2ml of different dilution factor, different ratio is added in test tube, then add 2ml76 μm of ol/L DPPH alcoholic solution, mixing, under room temperature, lucifuge is at dark place reaction 30min.Absorbance is measured at 525nm place.Each concentration in triplicate, is averaged as fitting data, to ensure the accuracy of experimental result.
Clearance rate I (%)=[1-(T-T0)/(C-C0)] × 100%
In formula: T0:2ml water or dimethyl sulfoxine (DMSO) add the absorbance of 2ml DPPH solution;
T:2ml sample liquid adds the absorbance of 2mlDPPH solution;
C0:2ml water or dimethyl sulfoxine (DMSO) add the absorbance of 2ml95% ethanol;
C:2ml sample liquid adds the absorbance of 2ml95% ethanol.
According to formulae discovery clearance rate above.Result of the test is as shown in table 1:
Table 1
| Sequence number | Sample | Concentration (ug/ml) | Clearance rate (%) |
| 1 | Black Fructus Lycii+black tomato (10: 1) | 5 | 10.8 |
| 2 | Black Fructus Lycii+black tomato (10: 1) | 10 | 21.7 |
| 3 | Black Fructus Lycii+black tomato (10: 1) | 25 | 33.9 |
| 4 | Black Fructus Lycii+black tomato (10: 1) | 50 | 74.5 |
| 5 | Black Fructus Lycii+black tomato (5: 1) | 50 | 70.5 |
| 6 | Black Fructus Lycii+black tomato (1: 1) | 50 | 50.3 |
| 7 | Black Fructus Lycii+black tomato (1: 5) | 50 | 44.8 |
| 8 | Black Fructus Lycii+black tomato (1: 10) | 50 | 31.9 |
As seen from the above table, the mixing under above-mentioned psammophytes extract different proportion all has the effect of certain scavenging free radicals to free radical.
2), Elastase Inhibitory research
Test method is according to document (Am.J.Pharmacol.Toxicol., 2009,4, method 127-129) is carried out, step is: in 96 orifice plates, add the 0.1M Tris-C1 buffer solution (PH=8.0) that 10uL sample solution and 130uL contain 1.015mM reaction substrate Succ-Ala-Ala-Ala-p-nitroanilide, incubation 5 minutes at 25 DEG C, add 15uL elastin laminin enzymatic solution (0.5U/ml), continue incubation 30min at 25 DEG C, then measure the absorbance A 410 of 410nm wavelength by microplate reader.Replace sample aqueous solution with deionized water, as reference solution, measure absorbance, by with matched group compare the relative inhibition obtaining sample sets.Result of the test is in table 2:
Table 2
| Sequence number | Sample | Concentration (ug/ml) | Suppression ratio (%) |
| 1 | Black Fructus Lycii+black tomato (10: 1) | 100 | 2.8 |
| 2 | Black Fructus Lycii+black tomato (10: 1) | 200 | 2.9 |
| 3 | Black Fructus Lycii+black tomato (10: 1) | 250 | 42.8 |
| 4 | Black Fructus Lycii+black tomato (10: 1) | 500 | 70.0 |
| 5 | Black Fructus Lycii+black tomato (5: 1) | 500 | 51.7 |
| 6 | Black Fructus Lycii+black tomato (1: 1) | 500 | 10.5 |
| 7 | Black Fructus Lycii+black tomato (1: 5) | 500 | 5.6 |
| 8 | Black Fructus Lycii+black tomato (1: 10) | 500 | 3.3 |
As seen from the above table, the mixing under above-mentioned psammophytes extract different proportion all has certain inhibitory action to elastoser.
3), fibroblast proliferation Effect study
Test method: by the cell suspension inoculation for preparing in 96 orifice plates, after cell attachment, except blank group, other organize the sample that every hole adds 20ul respective concentration, be placed in incubator and cultivate 24h, then add MTT liquid 20ul, continue to cultivate 4h in incubator.After taking out ELISA Plate, suck the culture fluid in every hole, add dimethyl sulfoxine mix homogeneously.Optical density (570nm) is measured, by obtaining the relative rate of increase of sample sets with the comparison of matched group with enzyme-linked immunosorbent assay instrument.
Result of the test is in table 3:
Table 3
As seen from the above table, the mixing under above-mentioned psammophytes extract different proportion all has certain proliferation function to fibroblast.
Embodiment 3: the whitening function research of psammophytes extract combination
1), tyrosinase inhibitory action research
Test method: the sample solution respectively adding 1ml variable concentrations in sample cell and sample controls pipe, positive and negative control pipe replaces with phosphate buffer.0.5ml tyrosinase solution (Mei Huo unit 125u/ml) is added in sample cell and positive control pipe, sample controls and negative control pipe replace with 0.5ml phosphate buffer (pH=6.8), the all test tubes of jolting, sample and tryrosinase are fully mixed, 37 DEG C of tanks hatch 10 minutes, add the 0.03wt% tyrosine solution of 2ml, react 10 minutes, be namely engraved in 475nm place and measure absorbance.
Suppression ratio I (%)=[1-(T-T0)/(C-C0)] × 100%
In formula: T0: sample solvent contrasts; T: sample controls; C: positive control; C0: positive control solvent control.
Result of the test is as shown in table 4:
Table 4
As seen from the above table, the mixing under above-mentioned psammophytes extract different proportion all has certain inhibitory action to tryrosinase.
2), to the research of melanin inhibitory action
The modification method such as Hosoi is adopted to measure extract to melanin inhibitory action.B16 melanocyte is cultivated in 96 orifice plates with 1 × 105 density, liquid is changed after 24h, add the medicine of variable concentrations, after 72h, wash twice through PBS, sample, through air drying, is dissolved in 200 μ l1N NAOH (containing 1%DMSO), cool after heating 80 DEG C to 1h, select 475nm wavelength to read absorbance on enzyme-linked immunosorbent assay instrument.
Melanin content suppression ratio=[1-(medicine hole absorbance/medicine porocyte density) (control wells absorbance/photograph porocyte density)] × 100%, result of the test is in table 5:
Table 5
| Sequence number | Sample | Concentration (ug/ml) | Suppression ratio (%) |
| 1 | Black Fructus Lycii+black tomato (10: 1) | 100 | 10.8±2.2 |
| 2 | Black Fructus Lycii+black tomato (10: 1) | 200 | 31.6±4.4 |
| 3 | Black Fructus Lycii+black tomato (10: 1) | 250 | 44.0±6.3 |
| 4 | Black Fructus Lycii+black tomato (10: 1) | 500 | 52.1±6.3 |
| 5 | Black Fructus Lycii+black tomato (5: 1) | 500 | 44.0±7.2 |
| 6 | Black Fructus Lycii+black tomato (1: 1) | 500 | 33.8±3.4 |
| 7 | Black Fructus Lycii+black tomato (1: 5) | 500 | 12.9±2.2 |
| 8 | Black Fructus Lycii+black tomato (1: 10) | 500 | 12.1±1.2 |
As seen from the above table, the mixing under above-mentioned psammophytes extract different proportion all has certain inhibitory action to B16 cell melanin propagation.
Embodiment 4: the anti-sunlight function research of psammophytes extract combination
1), UV protective action research
Get the mixture (10: 1) of black Fructus Lycii extract and black tomato extract, after the ethanol dilution with 70% to 25ml, do UV scanning with ultraviolet spectrophotometer at 200-400nm, scanning result as shown in Figure 1.Obtain at long wave ultraviolet (UVA, 320-400nm) and ultraviolet B radiation (UVB, 290-320nm) interval value with ultraviolet spectrophotometer sensing.As seen from the figure, the mixture of above-mentioned two kinds of psammophytes extracts has ultra-violet absorption effect, namely has UVA and UVB protective action.
2), UV is to fibroblast proliferation Experiment on Function
Principle: skin photoage causes jointly primarily of the ultraviolet B radiation (UVB, 290-320hm) in daylight and long wave ultraviolet (UVA, 320-400nm), ultraviolet has obvious facilitation to the aging of application on human skin.UVA or UVB irradiation can cause fibroblastic damage dead, damages, cause photoaging to skin.Irradiate experiment by UV and determine whether extract has protective effect to the cell that UV irradiates, resist photoaging effect.
Method of testing: the mixture of black Fructus Lycii extract and black tomato extract adds PBS buffer and dilutes, be made into 1/10 concentration (v/v) and carry out sucking filtration, then get 0.1ml and add 9.9ml sterile PBS buffer, be finally diluted to the concentration (v/v) of 1/1000.The skin flbroblast (Fb) of cultivating with normal DMEM liquid is for matched group, the Fb cell irradiated with UVA or UVB after cultivating with normal DMEM liquid for model group, with the Fb cell irradiated containing the embodiment extract of 1/1000 concentration and DMEM liquid Dual culture and through UVA or UVB for dosing group.24h after UV treatment, microplate reader measures the absorbance (0D value) in each hole, and cell proliferation rate is the percentage rate that each processed group OD value accounts for negative control group OD value.Result of the test is in table 6 (UVA irradiation) and table 7 (UVB irradiation):
Table 6
| Sequence number | Sample | Concentration (ug/ml) | The rate of increase (%) |
| 1 | Matched group | 500 | 98.7±6.5 |
| 2 | Model group | 500 | 68.2±5.8 |
| 3 | Black Fructus Lycii+black tomato (10: 1) | 100 | 20.3±2.9 |
| 4 | Black Fructus Lycii+black tomato (10: 1) | 200 | 33.9±3.9 |
| 5 | Black Fructus Lycii+black tomato (10: 1) | 250 | 58.9±6.7 |
| 6 | Black Fructus Lycii+black tomato (10: 1) | 500 | 82.7±7.2 |
| 7 | Black Fructus Lycii+black tomato (5: 1) | 500 | 72.9±7.1 |
| 8 | Black Fructus Lycii+black tomato (1: 1) | 500 | 59.8±4.8 |
| 9 | Black Fructus Lycii+black tomato (1: 5) | 500 | 40.2±3.7 |
| 10 | Black Fructus Lycii+black tomato (1: 10) | 500 | 21.1±3.2 |
Table 7
| Sequence number | Sample | Concentration (ug/ml) | The rate of increase (%) |
| 1 | Matched group | 500 | 98.6±9.2 |
| 2 | Model group | 500 | 78.8±8.9 |
| 3 | Black Fructus Lycii+black tomato (10: 1) | 100 | 60.3±5.5 |
| 4 | Black Fructus Lycii+black tomato (10: 1) | 200 | 63.0±6.0 |
| 5 | Black Fructus Lycii+black tomato (10: 1) | 250 | 75.9±6.1 |
| 6 | Black Fructus Lycii+black tomato (10: 1) | 500 | 82.7±7.2 |
| 7 | Black Fructus Lycii+black tomato (5: 1) | 500 | 72.3±5.0 |
| 8 | Black Fructus Lycii+black tomato (1: 1) | 500 | 59.0±3.7 |
| 9 | Black Fructus Lycii+black tomato (1: 5) | 500 | 55.2±5.4 |
| 10 | Black Fructus Lycii+black tomato (1: 10) | 500 | 51.1±8.1 |
As shown in table 6 and table 7, mixing under black Fructus Lycii extract and black tomato extract different proportion all has facilitation to the appreciation rate that Skin Cell recovers, therefore, the mixture of black Fructus Lycii extract and black tomato extract has certain inhibitory action to application on human skin because UVA and UVB irradiates the photoaging caused.
Embodiment 5: the moisture-keeping function research of psammophytes extract
1), moisture-keeping functions test
The mixture of black Fructus Lycii extract and black tomato extract is made into 1% (w/v) concentration, glycerol is made into the aqueous solution of 5% (w/v) concentration, select the surface plate of same specification, one deck medical air-permeable adhesive tape is pasted above, under room temperature condition, (24 DEG C) draw 200 μ L, even application is on breathable adhesive tape, then the exsiccator interior (relative humidity is about 50%) of potassium acetate saturated solution is put it into, place and weigh respectively after 5 hours, calculate moisturizing rate.Result of the test is as shown in table 8:
Table 8
| Sequence number | Sample | Concentration (ug/ml) | Moisturizing rate (%) |
| 1 | Glycerol (5%, contrast) | —— | 5.9±1.2 |
| 2 | Black Fructus Lycii+black tomato (10: 1) | 200 | 0.3±0.1 |
| 3 | Black Fructus Lycii+black tomato (10: 1) | 400 | 3.0±0.8 |
| 4 | Black Fructus Lycii+black tomato (10: 1) | 800 | 5.9±1.4 |
| 5 | Black Fructus Lycii+black tomato (10: 1) | 1000 | 7.6±1.8 |
| 6 | Black Fructus Lycii+black tomato (5: 1) | 1000 | 6.0±1.1 |
| 7 | Black Fructus Lycii+black tomato (1: 1) | 1000 | 4.7±1.0 |
| 8 | Black Fructus Lycii+black tomato (1: 5) | 1000 | 2.6±0.4 |
| 9 | Black Fructus Lycii+black tomato (1: 10) | 1000 | 2.1±0.8 |
As seen from the above table, the mixing under above-mentioned psammophytes extract different proportion all has certain moisture-keeping function.
2) in vitro tests of horn cell dehydration, is prevented
Principle: under epidermis cell is in dry environment, investigates extract active ingredient and helps cell to resist drying, maintain the ability of the original form of cell.
Method of testing: get each extract 0.1ml, adds MEM culture fluid 9.9ml, sucking filtration, is mixed with the concentration of 1%, same to method, prepares 1% glycerol as positive control.People's epidermal keratinocyte Hacat is selected to be experimental cell, with normal MEM liquid cultured cells for blank group, with the MEM liquid cultured cells containing 1% glycerol concentrations for positive controls, with the MEM liquid cultured cells of the embodiment extract containing 1% concentration for dosing group, after hatching 24h, blot every hole liquid successively, be placed in basis of microscopic observation horn cell from the normal time experienced to dry (state significant change).Result of the test is in table 9:
Table 9
| Sequence number | Sample | Concentration (ug/ml) | Hold time (s) |
| 1 | Blank | —— | 705±12 |
| 2 | 1% glycerol (contrast) | —— | 790±85 |
| 3 | Black Fructus Lycii+black tomato (10: 1) | 200 | 723±20 |
| 4 | Black Fructus Lycii+black tomato (10: 1) | 400 | 752±68 |
| 5 | Black Fructus Lycii+black tomato (10: 1) | 800 | 809±34 |
| 6 | Black Fructus Lycii+black tomato (10: 1) | 1000 | 842±78 |
| 7 | Black Fructus Lycii+black tomato (5: 1) | 1000 | 764±23 |
| 8 | Black Fructus Lycii+black tomato (1: 1) | 1000 | 577±30 |
| 9 | Black Fructus Lycii+black tomato (1: 5) | 1000 | 556±84 |
| 10 | Black Fructus Lycii+black tomato (1: 10) | 1000 | 550±68 |
As seen from the above table, the mixing under above-mentioned psammophytes extract different proportion can help people's epidermal keratinocyte to resist drying, namely has moisture-keeping function.
By above various test, the mixture of these two kinds of psammophytes extracts of visible black Fructus Lycii, black tomato has effect of good whitening, moisturizing, anti-ageing beauty and skin care of waiting for a long time.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.
Claims (9)
1. a psammophytes mixture, is characterized in that, is the mixture of black Fructus Lycii extract and black tomato extract.
2. psammophytes mixture as claimed in claim 1, it is characterized in that, in described mixture, the weight ratio of black Fructus Lycii extract and black tomato extract is 1-10: 1-10.
3. psammophytes mixture as claimed in claim 2, it is characterized in that, the weight ratio of described black Fructus Lycii extract and black tomato extract is 1-10: 1-5.
4. psammophytes mixture as claimed in claim 1, it is characterized in that, described psammophytes mixture is prepared by the following method: respectively black Fructus Lycii and black tomato raw material flooded organic solvent and carry out after extraction filtering, reduce pressure and concentrate rear acquisition black Fructus Lycii extract and black tomato extract, then being mixed by a certain percentage by these two kinds of extracts.
5. the psammophytes mixture as described in as arbitrary in claim 1-4, it is characterized in that, described psammophytes mixture has antioxidation.
6. the psammophytes mixture as described in as arbitrary in claim 1-4, it is characterized in that, described psammophytes mixture has whitening function.
7. the psammophytes mixture as described in as arbitrary in claim 1-4, it is characterized in that, described psammophytes mixture has anti-sunlight function.
8. the psammophytes mixture as described in as arbitrary in claim 1-4, it is characterized in that, described psammophytes mixture has moisture-keeping function.
9. the application of psammophytes mixture in cosmetics, health product or medicine as described in as arbitrary in claim 1-8.
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Cited By (1)
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| CN108434443A (en) * | 2018-05-28 | 2018-08-24 | 广东康侨药业有限公司 | A kind of whitening antioxidation composition and application thereof |
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