CN104263672B - One plant height produces Shewanella and the application thereof of electricity - Google Patents
One plant height produces Shewanella and the application thereof of electricity Download PDFInfo
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Abstract
The invention discloses a plant height and produce the Shewanella of electricity, its Classification And Nomenclature is Shewanella (Shewanella oneidensis), being preserved in China typical culture collection center, its deposit number is CCTCC No:M2014340, and preservation date is on July 16th, 2014.The invention also discloses the application in producing electricity of the above-mentioned high yield electric Shewanella.The present invention is to improve Shewanella electricity generation ability in MFC to provide a kind of research direction.The Shewanella of the present invention is when 20mM sodium lactate is as electron donor, and maximum output voltage is up to 449mV, improves 18.2% than initial strains MR 1, and peak power output density is 160mW/m2, improve 82.6% than initial strains MR 1.
Description
Technical field
The invention belongs to environmental pollution biological treatment and bioenergy technical field, be specifically related to a kind of Shewanella and produce the application on electricity at microbiological fuel cell.
Background technology
Energy shortage is the increasingly serious problem that China faces now, thus energy development becomes modern society of China and carries out a major challenge of sustainable development.Scientists constantly looks for new technical solution, wherein microbiological fuel cell (Microbial Fuel Cell, MFC) be exactly one of them for the new equipment producing fungible energy source, and its importance manifests the most day by day.
Meanwhile, a major challenge of the environmental pollution Ye Shi mankind modern society Faced In Sustainable Development caused in energy exploitation and application.And sewage recycling eliminates one of model reaching resource while polluting again just like one.
MFC is the device utilizing product electricity microorganism that the chemical energy in Organic substance is converted into electric energy as anode catalyst.From the perspective of the energy and environmental conservation, microbiological fuel cell is applied to field of waste water treatment, in being processed by MFC technology application waste water, while processing organic wastewater, obtains electric energy, be to alleviate current energy source crisis and solve the effective way of environmental problem.In such a system, thalline is this quality factor affecting MFC electricity generation performance, and in recent years, reports increasing about the microorganism utilizing MFC system to produce electric current.Although the bacterial strain that can produce electricity is more, but they most of electro-chemical activities are relatively low, and its electrical power produced is the lowest.Especially gram positive bacteria, not only one layer of oil film of extracellular parcel is rich in some insulating properties materials such as Peptidoglycans in non-conductive material and cell membrane, thus seriously hinders the electron exchange between microorganism and electrode, reduces the efficiency of MFC.Therefore, screen highly efficient electricity microorganism of producing and become the core of MFC technology.
Microorganism electricity generation ability differs greatly, produce electricity microorganism and decide function and the application of MFC, the product electricity microorganism having now been found that is concentrated mainly on genus Shewanella (Shewanella) and ground Bacillus (Geobacter) (Energy Environ.Sci., 2011,4,4,366 4379) some gram negative bacterias such as.Wherein Shewanella belongs to amphimicrobian heterotroph, and gas chromatography can be utilized as metabolism substrate.Along with the further investigation to product electricity microorganism, recently in MFC, produce electric report about Shewanella the most increasing.Waston and Logan (Biotechnology and Bioengineering, 2010,105 (3): 489 498) utilizing Shewanella oneidensis MR-1,18mM lactic acid is as electron donor, only produce voltage 120mV, output 40mW/m2；Produce electricity principle about Shewanella oneidensis MR-1 to resolve, Bretschger and Obraztsova et al. (Appl.Environ.Microbiol, 2007,73 (21): 7,003 7012) use clpp gene division, analyze the effect of cytochrome in electron transfer process.
Summary of the invention
The technical problem to be solved is to provide the Shewanella with high activity electricity generation ability that a strain becomes through ARTP plasma inducing.
The present invention also to solve the technical problem that the application being to provide above-mentioned Shewanella in terms of producing electricity.
For solving above-mentioned technical problem, the technical solution used in the present invention is as follows:
One plant height produces the Shewanella of electricity, and its Classification And Nomenclature is Shewanella (Shewanella oneidensis), has been preserved in China typical culture collection center, and its deposit number is CCTCC No:M2014340, and preservation date is on July 16th, 2014.
The screening technique of the Shewanella Shewanella oneidensis CCTCC No:M2014340 of the present invention, by Shewanella starting strain Shewanella oneidensis MR-1 after plasma mutation, utilize the screening of WO3 developing technology to obtain the bacterial strain of possible high yield electricity, then carry out screening the bacterial strain of high yield electric energy through dual chamber MFC.
It specifically comprises the following steps that
(1) plasma mutation: by Shewanella original strain MR-1 activation culture, cultivation temperature 28～30 DEG C, the centrifuge tube liquid amount of 50mL is 5～10mL, incubation time 12～16h, obtain being in the bacterium solution of exponential phase, the cell of cultivation is diluted to OD600=0.1～1.0, drip on the slide glass after sterilizing cools down, dry up with filtrated air；With helium as discharge gas, using 80～120W as radio-frequency power, using 10～30SLM as gas flow, using 10～1800s as exposure time, bacterial strain is carried out plasma mutation.
(2) screen at the beginning of sandwich style culture technique: the bacterial strain after mutation is respectively coated in solid medium, after 20min, is coated the aseptic WO of 25mL3Agar suspension, 30 DEG C of Anaerobic culturel, incubation time 24～36h, observe color change, picking blue portion bacterium colony, seed culture, preserve.
(3) 96 hole flat board developing technology are screened again: by the inoculation of above-mentioned collection in seed culture medium, after 30 DEG C of greenhouse shaking tables cultivate 12～16h, reaching logarithmic (log) phase bacterium solution, under 8000rpm, centrifugal 3min collects thalline, suspend with aseptic sodium lactate minimum saline solution, adjust cell OD600=1.0 is standby.Take the standby bacterium solution that 100ul contains sodium lactate minimum saline solution to add in 96 hole flat boards, then it is aseptic containing WO to be separately added into 80ul3The sodium lactate minimum saline solution of nanocluster (5g/L), adds 80ul vaseline immediately and guarantees anaerobic state, put into 30 DEG C of anaerobic box, 10,15,30,60min observe color change.
(4) dual chamber MFC technology screening: bacterial strain step (3) screened accesses seed liquor, cultivation temperature 28～30 DEG C, rotating speed 150rpm, incubation time 12～16h.Then carry out dual chamber MFC technology, be adjusted to thalline initial OD in anode chamber600=1.0, cultivation temperature 28～30 DEG C；Investigate the product electricity of the bacterial strain that step (3) filters out, select simultaneously and produce the bacterial strain that electricity is the highest.
(5) in above-mentioned screening technique: in the plasma mutation method described in step (1), preferably 100W is as radio-frequency power, and 10SLM is as gas flow, and 90s is as exposure time.
In above-mentioned screening technique: the solid medium described in step (2): peptone, yeast powder, sodium chloride, agar；Aseptic WO3Agar suspension comprises WO3Nanocluster, sodium salt, agar.
In above-mentioned screening technique: the seed liquor described in step (3) is that TSB aqueous solution comprises tryptone, soy peptone, meat soup；Sodium lactate minimum saline solution comprises sodium lactate, sodium salt, potassium salt, phosphate, hydroxyethyl piperazine ethanesulfonic acid and small-scale inorganic salt compound, respectively NTA, magnesium salt, manganese salt.Sodium salt, ferrous salt, calcium salt, cobalt salt, zinc salt, mantoquita, aluminium salt, nickel salt, tungsten thing, boric acid one or more, WO3The sodium lactate minimum saline solution of nanocluster, for adding WO in sodium lactate minimum saline solution3Nanocluster.
In above-mentioned screening technique: in step (4) described dual chamber MFC device, anolyte is potassium salt, phosphate, ammonium salt, and sodium lactate is as electron donor.Catholyte is potassium salt, phosphate, ferrum cyanogen compound.
Wherein, described dual chamber MFC technology is produced method for electrically and is comprised the steps of:
(1) bacterial strain activation: Shewanella Shewanella oneidensis CCTCC No:M2014340 is inoculated in TSB seed culture medium, cultivation temperature 28～30 DEG C, rotating speed 150rpm, incubation time 12～16h；
(2) device is built: dispose the anode of anode chamber, disposes the negative electrode of cathode chamber, disposes between anodal compartment and cathodic compartment for separating the PEM of anode chamber and cathode chamber, carries out sterilization treatment；
(3) MFC technology: add anolyte in anode chamber, access Shewanella Shewanella oneidensis CCTCC No:M2014340, anode chamber's initial OD600=1.0, seal rapidly anode chamber, add catholyte to cathode chamber, seal rapidly cathode chamber, as 30 DEG C of calorstats, carry out voltage data harvester and gather data.
Described anode and negative electrode are PAN base graphite soft felt (5 × 5cm), and using titanium silk as external circuits, outer meeting resistance is 1000 Ω, and PEM is Du Pont NafionN117.
Described anolyte is potassium salt, phosphate, ammonium salt, and sodium lactate is as electron donor.Catholyte is potassium salt, phosphate, ferrum cyanogen compound.
Mutant CCTCC No:M2014340 and the starting strain MR-1 obtained according to above-mentioned screening technique colonial morphology on solid medium, color and luster is almost identical understands, and still belongs to Shewanella through the mutant CCTCC No:M2014340 of mutation.
The application in producing electricity of the Shewanella of above-mentioned high yield electricity is also within protection scope of the present invention.
The Shewanella of above-mentioned high yield electricity as microbiological fuel cell in the application produced in electricity also within protection scope of the present invention.
Above-mentioned Shewanella carries out producing the concrete grammar of electricity as microbiological fuel cell, comprises the steps:
(1) microbiological fuel cell is built: join in anode chamber by the anolyte containing fuel, using high yield electricity Shewanella CCTCC No:M2014340 thalline as anode chamber's inoculum；Catholyte is joined in cathode chamber；
(2) utilize microbiological fuel cell to produce electricity: microbiological fuel cell is put into quiescent culture in the greenhouse of 28～30 DEG C, carry out producing electricity.
In step (1), in the described anolyte containing fuel, with sodium lactate as electron donor, the concentration of sodium lactate is 20 ± 0.2mmol/L, preferably 20mmol/L.
In step (1), anolyte optimization formula containing fuel is as follows: sodium lactate 20 ± 0.2mmol/L, ammonium chloride 0.31 ± 0.02g/L, two hypophosphite monohydrate sodium dihydrogen 2.772 ± 0.05g/L, disodium hydrogen phosphate dodecahydrate 11.5 ± 0.3g/L, potassium chloride 0.13 ± 0.01g/L, solvent is water, pH=7.
In step (1), described high yield electricity Shewanella CCTCC No:M2014340 thalline first passes through following manner activation in advance: be inoculated in TSB seed culture medium by the strain of 28～30v/v% glycerine water solution freezings, cultivation temperature 28～30 DEG C, rotating speed 150rpm, constant temperature culture thalline 12～16h, reach exponential phase of growth, transfer two grades for 5v/v% with inoculum concentration, cultivate 12～16h under the same conditions.Obtain the logarithmic (log) phase bacterium solution arrived, centrifugal, abandon supernatant, add physiological saline solution and shake up, recentrifuge, the most repeatedly clean 2 times, obtain bacterium mud,
In step (1), in initial anode chamber cell concentration OD600=1.0 ± 0.1.
In step (1), described catholyte optimization formula is as follows: two hypophosphite monohydrate sodium dihydrogen 2.772 ± 0.05g/L, disodium hydrogen phosphate dodecahydrate 11.5 ± 0.3g/L, potassium chloride 0.13 ± 0.01g/L, the potassium ferricyanide 40 ± 2mmol/L, solvent is water, pH=7..
Beneficial effect: compared with prior art, the present invention has the advantage that the inventive method
(1) using plasma mutation Shewanella of the present invention, filters out the bacterium colony being likely to be of high electricity generation ability first with " sandwich style " solid medium, recycles 96 hole flat board WO3Developing technology sift out can single bacterium colony bacterial strain of high yield electricity, finally utilize dual chamber MFC technology screening and verify the bacterial strain of high yield electricity.
(2) present invention is to improve Shewanella electricity generation ability in MFC to provide a kind of research direction.
(3) Shewanella of the present invention is when 20mM sodium lactate is as electron donor, and maximum output voltage is up to 449mV, improves 18.2% than initial strains MR-1, and peak power output density is 160mW/m2, improve 82.6% than initial strains MR-1.
Accompanying drawing explanation
The microorganism classification named Shewanella ML-5Shewanella oneidensis ML-5 of the present invention, depositary institution is China typical culture collection center, it is called for short CCTCC, address: China. Wuhan. Wuhan University, postcode: 430072, deposit number is CCTCC NO:M 2014340, and preservation date is on July 16th, 2014.
Fig. 1 is the plasma mutation Survival curves of Shewanella.
Fig. 2 is the microbiological fuel cell structure principle chart with Shewanella as anode catalyst.
Fig. 3 a is the Shewanella Shewanella oneidensis ML-5 of the present invention electroactive figure of product in 20mM sodium lactate anolyte.
Fig. 3 b is the initial strains MR-1 electroactive figure of product in 20mM sodium lactate anolyte.
Fig. 4 is that the present invention utilizes 20mM sodium lactate as the product electricity situation of electron donor with initial strains MR-1.
Detailed description of the invention
According to following embodiment, the present invention be may be better understood.But, as it will be easily appreciated by one skilled in the art that the content described by embodiment is merely to illustrate the present invention, and should be also without limitation on the present invention described in detail in claims.
Embodiment 1:
This example demonstrates that the method that Shewanella original strain is carried out first step plasma mutation.
The method that Shewanella original strain carries out first step plasma mutation is as follows:
By Shewanella Shewanella oneidensis MR-1 bacterial strain activation culture, cultivation temperature 28～30 DEG C, 50mL centrifuge tube liquid amount is 5～10mL, rotating speed 150rpm, and incubation time 12～16h obtains eugonic bacterium solution；Transferring two grades by 5v/v%, condition of culture is identical with activation.The cell taking fresh cultured is diluted to cell concentration OD600=1～1.5, drip on the slide glass after sterilizing cools down, dry up with filtrated air；With helium as discharge gas, using 100W as radio-frequency power, using 10SLM as gas flow, using 10～1800s as exposure time, bacterial strain is carried out plasma mutation, after mutation, the Mycoderma on carrier is eluted, calculate survival rate.Experimental result is as shown in Figure 1；As shown in Figure 1,90s is optimal mutation exposure time.
Embodiment 2:
The method that this example demonstrates that the Clostridium beijerinckii filtering out high yield electricity.
Wherein, the culture medium prescription used is as follows:
(1) TSB seed culture medium: 3g TSB/100ml water
(2) sandwich style culture medium is as follows:
A), solid medium: yeast powder 5g/L, peptone 10g/L, sodium chloride 10g/L, agar 15～20g/L.
b)、WO3Agar suspension: WO3Nanocluster 5g/L, sodium chloride 10g/L, agar 20g/L.
(3) sodium lactate minimum saline solution: sodium lactate 2.02g/L, sodium chloride 5.85g/L, hydroxyethyl piperazine ethanesulfonic acid 11.91g/L, sodium hydroxide 0.3g/L, ammonium chloride 1.498g/L, potassium chloride 0.097g/L, two hypophosphite monohydrate sodium dihydrogen 0.67g/L.
Additional trace element solution (ratio 1:1000): NTA (C6H9NO6) 1.5g/L, bitter salt 30g/L, Manganous sulfate monohydrate 5g/L, sodium chloride 10g/L, green vitriol 1g/L, CALCIUM CHLORIDE DIHYDRATE 1g/L, cobalt chloride hexahydrate 1g/L, zinc chloride 1.3g/L, copper sulfate pentahydrate 0.1g/L, 12 hydrated sulfuric acid potassium aluminum 0.1g/L, boric acid 0.1g/L, two molybdic acid hydrate sodium 0.25g/L, Nickel dichloride hexahydrate 0.25g/L, Disodium tungstate (Na2WO4) dihydrate 0.25g/L.
Added amine-group acid solution (ratio 1:1000): Pidolidone 2g/L, L-arginine 2g/L, DL-serine 2g/L.
Added vitamins solution (ratio 1:1000): biotin 2g/L, folic acid 2g/L, pyridoxine hydrochloride 10g/L, riboflavin 5g/L, niacin 5g/L, vitamin B55g/L, vitamin B120.1g/L, p-para-amino benzoic acid 5g/L, thioctic acid 5g/L.
Containing WO3The sodium lactate minimum saline solution of nanocluster: add WO3The sodium lactate minimum saline solution of nanocluster.
(4) dual chamber MFC Anodic liquid: sodium lactate 20mM, ammonium chloride 0.31g/L, two hypophosphite monohydrate sodium dihydrogen 2.772g/L, disodium hydrogen phosphate dodecahydrate 11.5g/L, potassium chloride 0.13g/L, pH=7.
Catholyte in dual chamber MFC: two hypophosphite monohydrate sodium dihydrogen 2.772g/L, disodium hydrogen phosphate dodecahydrate 11.5g/L, potassium chloride 0.13g/L, potassium ferricyanide 40mM, pH=7.
Screening step:
(1) screen at the beginning of sandwich style culture technique
Slide glass after mutation is placed in equipped with in the tool plug test tube of 1～2mL normal saline, acutely shakes, by the bacterial strain eluting on slide glass, be diluted to variable concentrations and coat on solid medium flat board, after 20 minutes, then in solid plate culture medium, be coated with the aseptic WO of 25mL3Agar suspension, 28～30 DEG C of Anaerobic culturel 24～36h, pick out the bacterium colony with color variation zone, seed culture medium is cultivated, and preserves.
(2) 96 hole flat board developing technology are screened again
Bacterial strain and the starting strain MR-1 of above-mentioned collection are inoculated in TSB seed culture medium, cultivation temperature 28～30 DEG C, rotating speed 150rpm, cultivate 12～16h；Under 8000rpm, centrifugal 3min collects thalline, suspends with aseptic sodium lactate minimum saline solution, adjusts cell OD600=1.0 is standby.Take the standby bacterium solution that 100ul contains sodium lactate minimum saline solution to add in 96 hole flat boards, then it is aseptic containing WO to be separately added into 80ul3(5g/L) the sodium lactate minimum saline solution of nanocluster, adds 80ul vaseline immediately and guarantees anaerobic state, put into 30 DEG C of anaerobic box, 10,15,30,60min observe color change, pick out 8 strains and quickly turn blue and saturate bacterial strain.
(3) dual chamber MFC technology screening
Select the bacterial strain obtained and starting strain MR-1 is inoculated in seed culture medium, cultivation temperature 28～30 DEG C, rotating speed 150rpm, incubation time 12～16h by above-mentioned, reach logarithmic (log) phase bacterium solution；Switching secondary seed, liquid amount 100mL in the triangular flask of 500ml, inoculum concentration 5mL, cultivation temperature 28～30 DEG C, rotating speed 150rpm, incubation time 12～16h；Taking the logarithm phase bacterium solution, 8000rpm is centrifuged 3min, abandons supernatant, adds physiological saline solution and shakes up, and recentrifuge the most repeatedly cleans 2 times, obtains bacterium mud；According to cell concentration OD in initial anode chamber600=1.0 are inoculated in anode chamber, seal rapidly anode chamber, access catholyte to cathode chamber, seal cathode chamber, outer meeting resistance 1000 Ω, cultivation temperature 28～30 DEG C, data acquisition.When the voltage produced reduces, utilize circuit tester and resistance box to measure output voltage immediately, be measured peak power power density and the maximum output voltage of bacterium and starting strain MR-1, as shown in table 1:
Table 1
The 8 plant mutant strains major parts obtained through twice screening are carrying out the technical maximum output voltage of dual chamber MFC and power density all increases, finishing screen selects the bacterial strain ML-5 of maximum output voltage and maximum power density, being preserved in China typical culture collection center, its deposit number is CCTCC No:M2014340.
Embodiment 3:
This example demonstrates that Shewanella utilizes the product electricity of 20mM sodium lactate to test as anode catalyst.
(1) microbiological fuel cell is built
The present embodiment establishes the microbiological fuel cell utilizing Shewanella to generate electricity for anode catalyst according to existing technology and method, as shown in Figure 2, including anode chamber, cathode chamber, PEM and four parts of external circuit.Anode electrode and cathode electrode are PAN base graphite soft felt (5 × 5cm), and using titanium silk as external circuits, outer meeting resistance is 1000 Ω, and PEM is Du Pont NafionN117, and using data acquisition unit is Keithley series.
Anolyte formula is as follows: sodium lactate 20mmol/L, ammonium chloride 0.31g/L, two hypophosphite monohydrate sodium dihydrogen 2.772g/L, disodium hydrogen phosphate dodecahydrate 11.5g/L, potassium chloride 0.13g/L, and solvent is water, pH=7.
Catholyte formula is as follows: two hypophosphite monohydrate sodium dihydrogen 2.772g/L, disodium hydrogen phosphate dodecahydrate 11.5g/L, potassium chloride 0.13g/L, potassium ferricyanide 40mmol/L, and solvent is water, pH=7.
(2) the high yield electricity bacterial strain CCTCC No:M2014340 and initial strains MR-1 that activation screening obtains in seed liquor, cultivation temperature 30 DEG C, the Anaerobic culturel time 12～16h.Then carry out 250mL dual chamber MFC technology, in anode chamber, access anolyte 250mL, then access bacterium mud so that anode chamber's initial OD600=1.0, seal rapidly anode chamber；Add 250mL catholyte to cathode chamber, seal rapidly cathode chamber, cultivation temperature 30 DEG C, carry out data acquisition, finally give result as shown in table 3:
Table 3
According to output voltage data, calculate electron recovery efficiency and obtain the polarization curve concrete outcome producing electricity as shown in Figure 3,4.Wherein, Shewanella Shewanella oneidensis CCTCC No:M2014340 maximum output voltage of the present invention is up to 449mV, improves 18.2% than initial strains MR-1, and peak power output density is 160mW/m2, improve 82.6% than initial strains MR-1.
Claims (9)
1. one plant height produce electricity Shewanella, its Classification And Nomenclature be Shewanella (Shewanella oneidensis), it being preserved in China typical culture collection center, its deposit number is CCTCC No:M2014340, and preservation date is on July 16th, 2014.
2. the Shewanella of the high yield electricity described in claim 1 application in producing electricity.
3. the Shewanella of the high yield electricity described in claim 1 as microbiological fuel cell in the application produced in electricity.
Application the most according to claim 3, it is characterised in that Shewanella carries out the method producing electricity as microbiological fuel cell, comprises the steps:
(1) microbiological fuel cell is built: join in anode chamber by the anolyte containing fuel, using high yield electricity Shewanella CCTCC No:M2014340 thalline as anode chamber's inoculum；Catholyte is joined in cathode chamber；
(2) utilize microbiological fuel cell to produce electricity: microbiological fuel cell is put into quiescent culture in the greenhouse of 28～30 DEG C, carry out producing electricity.
Application the most according to claim 4, it is characterised in that in step (1), in the described anolyte containing fuel, with sodium lactate as electron donor, the concentration of sodium lactate is 20 ± 0.2mmol/L.
6. according to the application described in claim 4 or 5, it is characterized in that, anolyte formula containing fuel is as follows: sodium lactate 20 ± 0.2mmol/L, ammonium chloride 0.31 ± 0.02g/L, two hypophosphite monohydrate sodium dihydrogen 2.772 ± 0.05g/L, disodium hydrogen phosphate dodecahydrate 11.5 ± 0.3g/L, potassium chloride 0.13 ± 0.01g/L, solvent is water, pH=7.
Application the most according to claim 4, it is characterized in that, in step (1), described high yield electricity Shewanella CCTCC No:M2014340 thalline first passes through following manner activation in advance: be inoculated in TSB seed culture medium by the strain of 28～30v/v% glycerine water solution freezings, cultivation temperature 28～30 DEG C, rotating speed 150rpm, constant temperature culture thalline 12～16h, reach exponential phase of growth, transfer two grades for 5v/v% with inoculum concentration, cultivate 12～16h under the same conditions.
Application the most according to claim 4, it is characterised in that in step (1), in initial anode chamber cell concentration OD600=1.0 ± 0.1.
Application the most according to claim 4, it is characterized in that, in step (1), described catholyte formula is as follows: two hypophosphite monohydrate sodium dihydrogen 2.772 ± 0.05g/L, disodium hydrogen phosphate dodecahydrate 11.5 ± 0.3g/L, potassium chloride 0.13 ± 0.01g/L, the potassium ferricyanide 40 ± 2mmol/L, solvent is water, pH=7.
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| CN108832163B (en) * | 2018-07-19 | 2021-02-19 | 西南大学 | Micro microbial fuel cell and preparation method thereof |
| CN109735527B (en) * | 2018-12-29 | 2022-08-23 | 江苏大学 | Preparation method and application of Shewanella-nano ferrous sulfide capsule |
| CN110791521A (en) * | 2019-09-29 | 2020-02-14 | 天津大学前沿技术研究院 | Electricity-generating recombinant Shewanella strain and construction method and application thereof |
| CN110791520A (en) * | 2019-09-29 | 2020-02-14 | 天津大学前沿技术研究院 | Electricity-generating recombinant Shewanella strain and construction method and application thereof |
| CN110904020A (en) * | 2019-09-29 | 2020-03-24 | 天津大学前沿技术研究院 | Electricity-producing Shewanella recombinant strain and construction method and application thereof |
| CN110702762B (en) * | 2019-10-25 | 2021-08-13 | 中国科学技术大学 | Method for quantitatively detecting bioelectronic medium in solution |
| CN110878278B (en) * | 2019-12-10 | 2022-07-26 | 合肥工业大学 | Engineering strain for treating salt-containing organic wastewater and application thereof |
| CN111808774A (en) * | 2020-07-24 | 2020-10-23 | 江苏师范大学 | Recyclable oxygen indicator and preparation method and application thereof |
| CN115786157A (en) * | 2022-05-12 | 2023-03-14 | 天津大学 | Shewanella cruciata Carassii-D5 and application thereof in power generation |
| CN115851442A (en) * | 2023-01-13 | 2023-03-28 | 浙江大学 | Anaerobic single cell separation rapid screening method for electrochemical active bacteria |
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