CN104255493A - Method for producing feather cockscomb thumb flower - Google Patents
Method for producing feather cockscomb thumb flower Download PDFInfo
- Publication number
- CN104255493A CN104255493A CN201410481833.4A CN201410481833A CN104255493A CN 104255493 A CN104255493 A CN 104255493A CN 201410481833 A CN201410481833 A CN 201410481833A CN 104255493 A CN104255493 A CN 104255493A
- Authority
- CN
- China
- Prior art keywords
- medium
- feather cockscomb
- flower
- seedling
- thumb
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The invention relates to a method for producing a feather cockscomb thumb flower. The method comprises the steps of selecting a feather cockscomb seed as an explant, inoculating the explant on the surface of a primary culture medium; cutting off a hypocotyl of a feather cockscomb seedling; discarding the hypocotyl and a root system; grafting a cotyledon to a flower bud induction medium; after culturing the cotyledon on the flower bud induction medium, grafting the cultured cotyledon to a florescence culture medium; cutting off yellow leaves and a root system at the base part of the feather cockscomb seedling on which the inflorescence appears, and grafting the feather cockscomb seedling to a freshness-retaining culture medium for culturing; cutting off the root part and leaves of the main stem of the base part of the feather cockscomb seedling, and inserting the feather cockscomb seedling to a breeding culture medium to obtain a sub-cultured seedling; selecting a stem section with a flower bud at the top end and vertically inserting the stem section into a rooting culture medium until roots grow so as to finish the culture of the thumb flower. The method for producing the feather cockscomb thumb flower overcomes the defects that the flowering time is uncontrollable, rapid breeding ways such as tissue culture cannot be adopted for production, and the flower period cannot be kept for a long time. According to the method for producing the feather cockscomb thumb flower, as the tissue culture way is used for breeding, the flowering condition can be controlled, the flowering time of the plant can be prolonged efficiently, and the thumb flowers can be produced rapidly and industrially.
Description
Technical field
The present invention relates to a kind of method of producing thumb flower, particularly a kind of method of producing feather cockscomb thumb flower.
Background technology
Thumb flower has another name called test tube flower, is utilize plant tissue culture fast breeding technique, and in closed glass vessel, by changing medium component and condition of culture, the manual control plant growth stage, that produces minily views and admires colored.
Test tube flowers more popularly in the market minily carry flowers, because its plant is little, longly to gain the name in the small test tube or vial of exquisiteness.It utilizes plant tissue culture technology, under the condition of relative or absolutesterility, prior cultured plant put into the general name of a series of mini plant that the large cuvette of thumb or art up vial grow.
The technology of test tube flower commercially available is at present also immature, is roughly divided into following a few class:
The first kind: simple type.Adopt the succulent that a kind of cactus with globular or elliptic stems, pin Rong, Lithops pseudotruncatella etc. are shorter and smaller and exquisite, be placed with in compost small container, namely finished product completes production.This type of plant easily takes root and poor growth, one or two months appearance without significant change.Production cost is low, and speed of production is fast, and production technology is simple.
Equations of The Second Kind: ornamental foliage plant.Adopt plantlet in vitro, cut 3 ~ 4cm branches and leaves and insert finished product medium, namely finished product completes production.This kind of vitroplant speed of production is fast, but without flower unrooted, the retention time is short.
3rd class: full plants.In closed container, the whole plant cultivated by tissue culture technology, is had root, has stem, has Ye Hehua.But there is morphological variation in this kind of test-tube plantlet great majority, is subject to Browning, the problems such as the rare and plant of kind is excessive.
Feather cockscomb belongs to Amaranthaceae (Amaranthacae), annual herb, high 0.3 ~ 1 meter, all without hair; Stem is upright, has branch; Blade square is justified lanceolar, lanceolar or is draped over one's shoulders needle-like bar shaped, long 5 ~ 8cm.Spend most close life, tower-like or cylindric spike, long 3 ~ 10cm, pink, color is simple and elegant.Be rich in rustic charm, color is lucid and lively.Growth distribution is extensive, and wild or cultivation, is easy to nursery.Feather cockscomb has sight and medical value, but the florescence is only between 5 ~ August, and plant height is higher.
Before the present invention makes, current test tube flowers technology Problems existing, be by controlling season, flowering time is uncontrollable, fast numerous modes such as group training can not be adopted to produce, also cannot keep the florescence long period, the colored plant of complete thumb of colored real below the 10cm that cannot cultivate root, have stem, have leaf, have.
Summary of the invention
Object of the present invention overcomes above-mentioned defect exactly, provides a kind of method of producing feather cockscomb thumb flower.
Technical scheme of the present invention is:
Produce a method for feather cockscomb thumb flower, it is characterized in that step is as follows:
(1) germination is cultivated: select celosia argentea L.seed as explant, be seeded in Initial culture primary surface; Described Initial culture base is: add sucrose 10 ~ 60g in the MS standard medium that macroelement reduces by half, carragheen 3 ~ 10g, indolebutyric acid 0.5 ~ 1.2mg, and water, to 1L, regulates pH to 5.8 ~ 6.2;
(2) Flower induction is cultivated: feather cockscomb seedling about 1cm below cotyledon is cut off hypocotyl, discards hypocotyl and root system, be transferred to by cotyledon on Flower induction medium; Described Flower induction medium is: MS standard medium adds sucrose 20 ~ 80g, carragheen 4 ~ 9g, abscisic acid 0.3 ~ 1.0mg, and water, to 1L, regulates pH to 5.8 ~ 6.2;
(3) flower is become to cultivate: feather cockscomb seedling is cultivated after 20 days on Flower induction medium, repaiies except root system is transferred on spent culture medium; Cultivate about 18 ~ 20 days, occur inflorescence successively; Described one-tenth spent culture medium is: modified MS medium, N content is reduced to 1/30 ~ 1/5 of MS medium, P content is increased to 3 ~ 10 times of MS medium, B content is increased to 3 ~ 10 times of MS medium, adds sucrose 35g, carragheen 10g, basic element of cell division 6-benzyladenine 0.01 ~ 0.08mg, indolebutyric acid 0.05 ~ 0.12mg, water, to 1L, regulates pH to 6.2;
(4) fresh-keeping cultivation: by occurring that the feather cockscomb seedling of inflorescence cuts away yellow leaf and the root system of base portion, being transferred on fresh-keeping medium, cultivating 15 ~ 20 days; Described fresh-keeping medium is: MS standard medium adds sucrose 10 ~ 60g, carragheen 3 ~ 10g, basic element of cell division 6-benzyladenine 0.01 ~ 0.08mg, and water, to 1L, regulates pH to 5.8 ~ 6.2;
(5) Multiplying culture: by the base portion of feather cockscomb seedling in fresh-keeping medium, cut the leaf of root and trunk, vertically inserts in proliferated culture medium, cultivates 20 ~ 30 days, can obtain the Regenerated plant of 4 ~ 10 times fast; Described proliferated culture medium is: MS standard medium adds sucrose 10 ~ 60g, carragheen 3 ~ 10g, basic element of cell division 6-benzyladenine 0.1 ~ 0.5mg, indolebutyric acid 0.05 ~ 0.3mg, and water, to 1L, regulates pH to 5.8 ~ 6.2;
(6) culture of rootage: choose the feather cockscomb seedling cultivated and grow petal in fresh-keeping medium and proliferated culture medium, two sections are cut into from the stem of distance petal 2cm, top is vertically inserted on root media with the stem section of petal, cultivates the palpus that can send out roots for 7 ~ 15 days, and the cultivation of thumb flower completes; Root media is: MS standard medium adds sucrose 20 ~ 40g, carragheen 3 ~ 10g, basic element of cell division 6-benzyladenine 0.01 ~ 0.2mg, chlormequat 1 ~ 5mg, and water, to 1L, regulates pH to 5.8 ~ 6.2.
Described culture environment condition is: temperature 25 ± 2 DEG C, and intensity of illumination 2000 ± 500lux, light application time is not less than 6 ~ 12h every day.
Feather cockscomb seedling in described step (2) to be seedling age the be feather cockscomb seedling of 20 ~ 25 days.
In described step (5) after the leaf cutting root and trunk, every plant is cut into 2 ~ 3 sections, every section has 2 ~ 4 axillalry buds.
Advantage of the present invention and effect there are provided a kind of using-system cultural method and cultivate the method that thumb is spent fast, industrialization is produced that can control flowering conditions, effectively extend the flowering of plant time.The feather cockscomb thumb flower moulding of producing is mini lovely, and flowering time reaches two months, and flowering time can be controlled by the hydrothermal condition in thumb flower container, has sight and the practicality of fresh flower concurrently.Thumb spend well overcome current test tube flowers market exist scarce limit, have the florescence long, insensitive, adaptable to container external environment, only have the advantages such as thumbnail size, flowering time can control.
Other concrete advantages of the present invention and effect will go on to say below.
Embodiment
Technical thought of the present invention is:
Different plant hormone is used to carry out integrated treatment to plant, the principle of regulating plant growth progress, the more complete thumb flower plant of in conjunction with the further dwarfed plant of cut-flower, producing root, having stem, having leaf, have colored real below 10cm.By control culture medium prescription and internal tank thermal and hydric environment, plant is bloomed according to artificial setting-up time simultaneously, and well can be kept at a period of time in thumb flower container.
First utilize outer execute abscisic acid concentration large time suppress the mechanism of action of stem, hypocotyl, root, coleoptile or leaf growth, abscisic acid process is carried out to the feather cockscomb seedling cultivated, by feather cockscomb dwarfization, the dwarfization plant that generation 3 ~ 5cm is high.The photosynthesis of treated feather cockscomb seedling leaf strengthens, and plant grows up and accelerates, and comes to the ripening period in advance.
Then flower process is become to these feather cockscomb seedlings, change the content of the nitrogen of medium, phosphorus and boron, to thumb seedling optimum flowering conditions.
Cheng Huahou, then by the further dwarfed plant of cut-flower.For occurring that test tube flowers have colored unrooted phenomenon at present, utilize chlormequat that crop cell can be suppressed to extend, but the principle of not T suppression cell division, we have developed and can plant be allowed fast to take root, the fresh-keeping medium of taking root that plant keeps miniaturization can be controlled again, produce root, have stem, have leaf, have the complete thumb flower plant of colored real below 10cm.
In order to realize object of the present invention, the present invention is cultivated by seed selection, the surface of the seed cleaning, the surface of the seed sterilization, cultivation of germinateing, Flower induction, is become nine steps such as flower cultivation, fresh-keeping cultivation, Multiplying culture, culture of rootage to implement.
Below in conjunction with some embodiments, the present invention will be further elaborated.But be not limited only to the scope of secondary embodiment as foregoing invention.
1. choose seeds
Select the celosia argentea L.seed that black is full, remove seed coat and other impurity, so that cleaning.
2. the surface of the seed cleaning
Seed is placed in 200ml triangular flask, adds 5 ~ 8ml liquid detergent, then add 100 ~ 120ml running water, repeatedly shake, after 5 ~ 8 minutes, remove liquid detergent solution, then inject running water and shake and wash 1 ~ 2 minute, remove cleaning solution, repeat 2 times.This flow process object is to remove the surface of the seed wax coat, and empty flat seed is removed in drift in cleaning process.
3. the surface of the seed sterilization
Adopt three step sterilizations: with 0.3% potassium permanganate by cleaned seed disinfection 15 minutes, remove liquor potassic permanganate, with sterile water, seed and triangular flask are rinsed 3 times.Use the ethanol of 75% again to seed through row surface sterilization 1min, discard ethanol disinfection liquid.Then carry out the surface sterilization of 15 minutes with the mercuric chloride solution of 0.15%, period stirs several times with aseptic nipper.Finally discard mercuric chloride thimerosal, with sterile water, seed and triangular flask are cleaned 4 ~ 5 times.The object of this flow process is the bacterium of taking out the surface of the seed, can stablize Fast-propagation in aseptic culture medium.
4. Initial culture
Seed through surface sterilization is evenly seeded in Initial culture primary surface, and after 1 ~ 2 day, seed can germinate.Temperature 25 ± 2 DEG C, intensity of illumination 2000 ± 500lux, under light application time is not less than 6 ~ 12h condition every day, cultivates 20 ~ 25 days.Initial culture base is: add sucrose 10 ~ 60g in the MS standard medium that macroelement reduces by half, carragheen 3 ~ 10g, indolebutyric acid 0.5 ~ 1.2mg, and water, to 1L, regulates pH to 5.8 ~ 6.2.
5. Flower induction is cultivated
Be feather cockscomb seedling about 1cm cut-out hypocotyl below cotyledon of 20 ~ 25 days by seedling age, discard hypocotyl and root system, cotyledon and growing point are transferred on Flower induction medium.Discard hypocotylar object in operating process to be feather cockscomb seedling is downgraded, the thumb flower that height of formation is suitable.Temperature 25 ± 2 DEG C, intensity of illumination 2000 ± 500lux, under light application time is not less than 6 ~ 12h condition every day, cultivates 20 days.Flower induction medium is: MS standard medium adds sucrose 20 ~ 80g, carragheen 4 ~ 9g, abscisic acid 0.3 ~ 1.0mg, and water, to 1L, regulates pH to 5.8 ~ 6.2.
6. become flower to cultivate
Feather cockscomb seedling is cultivated after 20 days on Flower induction medium, repaiies except root system is transferred on spent culture medium.One-tenth spent culture medium is modified MS medium, and the content of nitrogen wherein, phosphorus, boron element has adjustment in a big way, mainly in order to induce feather cockscomb seedling to bloom relative to general MS medium.Temperature 25 ± 2 DEG C, intensity of illumination 2000 ± 500lux, under light application time is not less than 6 ~ 12h condition every day, this one-tenth spent culture medium is cultivated about 18 ~ 20 days, namely can be observed inflorescence and occur successively.One-tenth spent culture medium is: modified MS medium, N content is reduced to original 1/30 ~ 1/5, P content is increased to original 3 ~ 10 times, B content is increased to original 3 ~ 10 times, adds sucrose 35g, carragheen 10g, basic element of cell division 6-benzyladenine 0.01 ~ 0.08mg, indolebutyric acid 0.05 ~ 0.12mg, water, to 1L, regulates pH to 6.2.
7. fresh-keeping cultivation
By occurring that the feather cockscomb seedling of inflorescence cuts away yellow leaf and the root system of base portion, being transferred on fresh-keeping medium, cultivating 15 ~ 20 days.Temperature 25 ± 2 DEG C, intensity of illumination 2000 ± 500lux, under light application time is not less than 6 ~ 12h condition every day, cultivate after 3 days, the leaf of feather cockscomb seedling and limb turn green gradually, and flower color also turns gorgeous gradually.Fresh-keeping medium is: MS standard medium adds sucrose 10 ~ 60g, carragheen 3 ~ 10g, basic element of cell division 6-benzyladenine 0.01 ~ 0.08mg, and water, to 1L, regulates pH to 5.8 ~ 6.2.
8. Multiplying culture
By the base portion of feather cockscomb seedling in fresh-keeping medium, cut the leaf of root and trunk, but can not axillalry bud be destroyed.Again every plant is cut into 2 ~ 3 sections, every section has 2 ~ 4 axillalry buds, vertically inserts in proliferated culture medium.Temperature 25 ± 2 DEG C, intensity of illumination 2000 ± 500lux, under light application time is not less than 6 ~ 12h condition every day, cultivates 20 ~ 30 days.Axillalry bud grows up to the side shoot of 2 ~ 3cm, is cut to be transferred to by side shoot to lure spent culture medium, can obtain the Regenerated plant of 4 ~ 10 times fast, and the thumb flower that Regenerated plant can carry out a new round is again cultivated.Proliferated culture medium is: MS standard medium adds sucrose 10 ~ 60g, carragheen 3 ~ 10g, basic element of cell division 6-benzyladenine 0.1 ~ 0.5mg, indolebutyric acid 0.05 ~ 0.3mg, and water, to 1L, regulates pH to 5.8 ~ 6.2.
9. culture of rootage
Choose the feather cockscomb seedling cultivated and grow petal in fresh-keeping medium and proliferated culture medium, be cut into two sections from the stem of distance petal 2cm, top is vertically inserted on the root media containing chlormequat with the stem section of petal.Here the object of cut-flower is to keep the further miniaturization of flower seedling.Temperature 25 ± 2 DEG C, intensity of illumination 2000 ± 500lux, under light application time is not less than 6 ~ 12h condition every day, cultivates the palpus that can send out roots for 7 ~ 15 days, and the cultivation of thumb flower completes, and can keep viewing and admiring about 2 months.Root media is: MS standard medium adds sucrose 20 ~ 40g, carragheen 3 ~ 10g, basic element of cell division 6-benzyladenine 0.01 ~ 0.2mg, chlormequat 1 ~ 5mg, and water, to 1L, regulates pH to 5.8 ~ 6.2.
Embodiment:
Directly gather wild celosia argentea L.seed in this example, filter out full seed by sieve, cleaning and the seed after sterilizing are seeded on Initial culture base, cultivate, illumination every day 8h under being placed in 25 ± 2 DEG C and 2000 ± 500lux illumination condition.
Be feather cockscomb seedling about 1cm cut-out hypocotyl below cotyledon of 20 days by seedling age, discard hypocotyl and root system, cotyledon and growing point are transferred on Flower induction medium.In this example, Flower induction medium is: MS minimal medium adds sucrose 20g, carragheen 6g, abscisic acid 0.3mg, and water, to 1L, regulates pH to 5.8; Feather cockscomb seedling is cultivated under being placed in 25 ± 2 DEG C and 2000 ± 500lux illumination condition, illumination every day 8 hours, cultivates 20 days.
Feather cockscomb seedling through Flower induction is repaiied except root system, is then transferred on spent culture medium.In this example, become spent culture medium to be preferably: modified MS medium, N content is reduced to 1/25, P original content and is increased to original 2 times, B content is increased to original 3 times, adds sucrose 30g, carragheen 10g, basic element of cell division 6-benzyladenine 0.08mg, water, to 1L, regulates pH to 6.2.Feather cockscomb seedling is cultivated under being placed in 25 ± 2 DEG C and 2000 ± 500lux illumination condition, illumination every day 8 hours, cultivates 15 days.
To become the growth feather cockscomb of 15 days in spent culture medium to repair yellow leaf and the root system of base portion, be transferred in fresh-keeping medium and continue to cultivate, in about 8 days, more than 95% titbit becomes redness; In this example, fresh-keeping medium is: MS minimal medium adds sucrose 20g, carragheen 6g, 6-benzyladenine 0.03mg, and water, to 1L, regulates pH to 5.8; Feather cockscomb seedling is cultivated under being placed in 25 ± 2 DEG C and 2000 ± 500lux illumination condition, illumination every day 8 hours, cultivates 25 days.
By the base portion of feather cockscomb seedling in fresh-keeping medium, the leaf cutting root and trunk inserts proliferated culture medium, but can not destroy axillalry bud.In this example, every plant is cut into 2 sections, every section has 4 axillalry buds, inserts proliferated culture medium.Cultivate 30 days, each axillalry bud can grow up to a Regenerated plant.Cultivate under being placed in 25 ± 2 DEG C and 2000 ± 500lux illumination condition, illumination every day 8h, has bred altogether the Regenerated plant of 4 times.
By having grown the feather cockscomb seedling of petal in fresh-keeping medium and proliferated culture medium, cutting the stem from petal 2cm, having retained 3 leaves, then vertically inserting root media.In this example, root media is: MS standard medium adds sucrose 40g, carragheen 6g, basic element of cell division 6-benzyladenine 0.1mg, chlormequat 2mg, and water, to 1L, regulates pH to 5.8.Cultivate under being placed in 25 ± 2 DEG C and 2000 ± 500lux illumination condition, illumination every day 8h, obtain the mini feather cockscomb of 3cm after within 10 days, taking root, pattern is bright-coloured, can keep the viewing period of 60 days.
The feather cockscomb thumb flower of group training, highly about 0.03 meter.The feather cockscomb of outdoor self-sow, highly about 1 meter.
These are only the embodiment of patent of the present invention; method in patent of the present invention all obtains consistent effect with formula in various great many of experiments; identical result is also obtain at the replica test in different time and place; therefore protection scope of the present invention is not only confined to this, any without creationary change and replace all should be encompassed in scope.Therefore, protection scope of the present invention should be as the criterion with claims limited range.
Claims (4)
1. produce a method for feather cockscomb thumb flower, it is characterized in that step is as follows:
(1) germination is cultivated: select celosia argentea L.seed as explant, be seeded in Initial culture primary surface; Described Initial culture base is: add sucrose 10 ~ 60g in the MS standard medium that macroelement reduces by half, carragheen 3 ~ 10g, indolebutyric acid 0.5 ~ 1.2mg, and water, to 1L, regulates pH to 5.8 ~ 6.2;
(2) Flower induction is cultivated: feather cockscomb seedling about 1cm below cotyledon is cut off hypocotyl, discards hypocotyl and root system, be transferred to by cotyledon on Flower induction medium; Described Flower induction medium is: MS standard medium adds sucrose 20 ~ 80g, carragheen 4 ~ 9g, abscisic acid 0.3 ~ 1.0mg, and water, to 1L, regulates pH to 5.8 ~ 6.2;
(3) flower is become to cultivate: feather cockscomb seedling is cultivated after 20 days on Flower induction medium, repaiies except root system is transferred on spent culture medium; Cultivate about 18 ~ 20 days, occur inflorescence successively; Described one-tenth spent culture medium is: modified MS medium, N content is reduced to 1/30 ~ 1/5 of MS medium, P content is increased to 3 ~ 10 times of MS medium, B content is increased to 3 ~ 10 times of MS medium, adds sucrose 35g, carragheen 10g, basic element of cell division 6-benzyladenine 0.01 ~ 0.08mg, indolebutyric acid 0.05 ~ 0.12mg, water, to 1L, regulates pH to 6.2;
(4) fresh-keeping cultivation: by occurring that the feather cockscomb seedling of inflorescence cuts away yellow leaf and the root system of base portion, being transferred on fresh-keeping medium, cultivating 15 ~ 20 days; Described fresh-keeping medium is: MS standard medium adds sucrose 10 ~ 60g, carragheen 3 ~ 10g, basic element of cell division 6-benzyladenine 0.01 ~ 0.08mg, and water, to 1L, regulates pH to 5.8 ~ 6.2;
(5) Multiplying culture: by the base portion of feather cockscomb seedling in fresh-keeping medium, cut the leaf of root and trunk, vertically inserts in proliferated culture medium, cultivates 20 ~ 30 days, can obtain the Regenerated plant of 4 ~ 10 times fast; Described proliferated culture medium is: MS standard medium adds sucrose 10 ~ 60g, carragheen 3 ~ 10g, basic element of cell division 6-benzyladenine 0.1 ~ 0.5mg, indolebutyric acid 0.05 ~ 0.3mg, and water, to 1L, regulates pH to 5.8 ~ 6.2;
(6) culture of rootage: choose the feather cockscomb seedling cultivated and grow petal in fresh-keeping medium and proliferated culture medium, two sections are cut into from the stem of distance petal 2cm, top is vertically inserted on root media with the stem section of petal, cultivates the palpus that can send out roots for 7 ~ 15 days, and the cultivation of thumb flower completes; Root media is: MS standard medium adds sucrose 20 ~ 40g, carragheen 3 ~ 10g, basic element of cell division 6-benzyladenine 0.01 ~ 0.2mg, chlormequat 1 ~ 5mg, and water, to 1L, regulates pH to 5.8 ~ 6.2.
2. a kind of method of producing feather cockscomb thumb flower according to claim 1, it is characterized in that culture environment condition is: temperature 25 ± 2 DEG C, intensity of illumination 2000 ± 500lux, light application time is not less than 6 ~ 12h every day.
3. a kind of method of producing feather cockscomb thumb flower according to claim 1, the feather cockscomb seedling that it is characterized in that in step (2) to be seedling age the be feather cockscomb seedling of 20 ~ 25 days.
4. a kind of method of producing feather cockscomb thumb flower according to claim 1, is characterized in that every plant is cut into 2 ~ 3 sections, and every section has 2 ~ 4 axillalry buds in step (5) after the leaf cutting root and trunk.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410481833.4A CN104255493B (en) | 2014-09-17 | 2014-09-17 | A kind of method of producing feather cockscomb thumb flower |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410481833.4A CN104255493B (en) | 2014-09-17 | 2014-09-17 | A kind of method of producing feather cockscomb thumb flower |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104255493A true CN104255493A (en) | 2015-01-07 |
| CN104255493B CN104255493B (en) | 2015-11-25 |
Family
ID=52147181
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410481833.4A Active CN104255493B (en) | 2014-09-17 | 2014-09-17 | A kind of method of producing feather cockscomb thumb flower |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104255493B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104759460A (en) * | 2015-04-15 | 2015-07-08 | 桂林理工大学 | Method for enhancing feather cockscomb manganese remediation efficiency |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100419740C (en) * | 2004-08-06 | 2008-09-17 | 佳能株式会社 | Information processing apparatus and notification method therefor, program for implementing the method, and storage medium storing the program |
-
2014
- 2014-09-17 CN CN201410481833.4A patent/CN104255493B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100419740C (en) * | 2004-08-06 | 2008-09-17 | 佳能株式会社 | Information processing apparatus and notification method therefor, program for implementing the method, and storage medium storing the program |
Non-Patent Citations (2)
| Title |
|---|
| 孙宁等: "外源激素对鸡冠花离体培养及试管成花的影响", 《中国农学通报》 * |
| 韩晓磊等: "青葙愈伤组织诱导研究", 《人参研究》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104759460A (en) * | 2015-04-15 | 2015-07-08 | 桂林理工大学 | Method for enhancing feather cockscomb manganese remediation efficiency |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104255493B (en) | 2015-11-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104885773B (en) | A kind of method of quickly breeding blue berry early stage sizing tissue culture commercial seedling | |
| CN103190347B (en) | Teapot dates tissue culturing method | |
| CN103704130A (en) | Chinese orchid and cymbidium hybridum hybrid seedling raising method | |
| CN103299911A (en) | Method for obtaining virus-free seedlings of pure cymbidium efficiently | |
| CN109287486B (en) | Paphiopedilum seed germination rate improving method and paphiopedilum cultivation method | |
| CN109392712A (en) | A kind of tissue culture and rapid propagation method of tara vine kind | |
| CN109287487B (en) | Seed germination rate improving method and cultivation method for paphiopedilum makino | |
| CN107079817A (en) | Pocket Lanzhou and Xinjiang kind " excellent pocket is blue " tissue culture and rapid propagation method | |
| CN107197746A (en) | A kind of mating system of China fir field excellent resources | |
| CN107027627A (en) | A kind of micro-tuber propagation method of David's-harp IMMATURE EMBRYOS CULTURE | |
| CN100394845C (en) | In-bottle production method of detoxified small seed ball of east lily | |
| CN109819892A (en) | A kind of method for tissue culture of tsaoko fine individual plant | |
| CN103314862A (en) | High-efficiency method for obtaining cymbidium detoxified seedling | |
| CN101564010B (en) | Method for rapidly propagating tupelos | |
| CN100391333C (en) | Aseptic seedling tissue culturing and test tube seedling hardening off and transplating technology for anthurium andraeanum | |
| CN105379621A (en) | Efficient in-vitro plant regeneration method of adult high-quality single-plant Xiaoqiao oriental cherry of cerasus lannesiana var. speciosa | |
| CN105284622A (en) | Method for rapid acquisition of excellent hybrid clone of iris | |
| CN104255493B (en) | A kind of method of producing feather cockscomb thumb flower | |
| CN104604735A (en) | Tissue culture and rapid propagation method for American lagerstroemia indica pink velour | |
| CN103858768A (en) | Tissue culture method of plumeria rubra L.cv.Acutifolia | |
| CN103931499A (en) | Tissue culture rapid propagation method for callistemon rigidus | |
| CN110771512B (en) | Efficient induction method of rabdosia lophanthide callus | |
| CN103704140A (en) | Method for obtaining polyploidy paulownia tomentosa by performing tissue culture propagation by taking young flowers as explants | |
| CN107155882A (en) | A kind of medicinal bletilla striata aseptic seeding fast seedling-cultivating method | |
| CN107094615A (en) | A kind of hybridizing method for overcoming lily interspecific hybridization obstacle |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20160921 Address after: 211800 Pukou Economic Development Zone, Jiangsu, Pu Tian Road, No. 5, No. Patentee after: Nanjing Business Consulting Co., Ltd. Address before: 211800 Pukou Economic Development Zone, Jiangsu, Nanjing Pu Road, No. 5 Patentee before: Nanjing Potomac Beauty&Health Care Co., Ltd. |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20190409 Address after: 571900 Mountainous Belly Ridge of Meiting Villagers Group, Meiting Village, Jinjiang Town, Chengmai County, Hainan Province Patentee after: Hainan Yuanyuan Biotechnology Co., Ltd. Address before: 211800 No. 5 Tianpu Road, Pukou Economic Development Zone, Nanjing City, Jiangsu Province Patentee before: Nanjing Business Consulting Co., Ltd. |