CN1042544A - The extraction method of the principal constituent of Longhairy Antenoron Herb (the pure and mild flavonoid glycoside of triterpene Chinese honey locust) - Google Patents
The extraction method of the principal constituent of Longhairy Antenoron Herb (the pure and mild flavonoid glycoside of triterpene Chinese honey locust) Download PDFInfo
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- CN1042544A CN1042544A CN 88107721 CN88107721A CN1042544A CN 1042544 A CN1042544 A CN 1042544A CN 88107721 CN88107721 CN 88107721 CN 88107721 A CN88107721 A CN 88107721A CN 1042544 A CN1042544 A CN 1042544A
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Abstract
Longhairy Antenoron Herb is a kind of active drug of preventing and treating urinary calculus, cholelithiasis.The present invention adopts new-type extracting method, and its principal constituent flavonoid glycoside and triterpene Chinese honey locust alcohol are purified out, makes it to make a kind of safer, stable, effective urinary calculi control medicine.
Description
The present invention is a kind of extracting method of effective ingredient of lithangiuria control medicine.
Longhairy Antenoron Herb (Desmodium styracifolium(Osb.) Merr) is leguminous plants, mainly produces in Guangdong, ground such as Guangxi, Fujian that it is sold on market with the active drug of treatment urinary calculus and cholelithiasis.At present China is mainly with herbal medicine be made into fluid extract sheet (logical with urolithiasis is representative) and form and be applied to clinical treatment.It is generally acknowledged in the past that it can increase coronary blood flow, promoted the effect of choleresis.And the effect of urinary calculus and the major ingredient of Longhairy Antenoron Herb are lacked systematic study.Because the composition of herbal medicine is subjected to the influence in physical environment, the place of production, so clinical efficacy is uncertain, instability.
Since the World War II, China is with the same all over the world, and the trend that increases year by year appears in the sickness rate of urinary calculus, and becomes the first disease of each province, China south Urology Surgery.Although the clinical application of extracorporeal shock wave and PNL, ureterorenoscope stone-extraction operation in recent years, also obtained bigger progress clinically, but the problems such as recurrence of the residual and calculus of postoperative calculus still are the major issues of Urology Surgery, so present countries in the world are all at the control medicine of researching and developing new urinary calculus.
The objective of the invention is needs according to urinary calculus treatment at present, the main composition of China Longhairy Antenoron Herb commonly used among the people is extract clinical prevention medication as urinary calculus, make it both avoid the effective ingredient wild effect of sward medicine, avoided again causing its main component content inconsistent, caused the bad phenomenon of clinical efficacy owing to the place of production of sward medicine is different.The major ingredient that the present invention extracts from Longhairy Antenoron Herb has two kinds: flavonoid glycoside and triterpene Chinese honey locust alcohol, flavonoid glycoside are the aromatics (Desmodium styracifolium-aromatic compoud is hereinafter to be referred as Ds-a) in the Longhairy Antenoron Herb; Triterpene Chinese honey locust alcohol (Desmodium styracifolium-Triterpenoid sapon-in is hereinafter to be referred as Ds-t).In the composition experimental study of Longhairy Antenoron Herb, find that its major ingredient has tangible diuretic properties, the excretion of calcium reduces in the urine, and the citric acid excretion increases, transaminase and creatinine no change in the blood.Main composition can also make by ethylene glycol, activated vitamin D
3The urinary calculi rate of formation of the urinary tract calcinm oxalate calculus animal model that causes obviously reduces, and can alleviate ethylene glycol, activated vitamin D
3Detrimental effect to renal function.Prove that promptly its main composition can reduce calcic urinary calculi significant risk factor urine calcium that forms and the excretion of urinating mesoxalic acid; increase the excretion that contains calcium calculus supressor citric acid; the diuretic properties that appearance forms the prevention urinary calculi; have the urinary calculi of preventing and form, especially the effect of calcinm oxalate calculus formation and the effect of renal function protecting.
Concrete extracting method of the present invention: the herb of Longhairy Antenoron Herb also flows extraction with rudimentary methyl alcohol or ethanol, and concentrating under reduced pressure is removed the solvent of Extract, and the fluid extract of gained distributes with equivalent n-fourth alcohol and water, gets the fluid extract sephadex LH of butanols layer
20(Sephadex) the post layer chromatography separates, and obtains four big separate partly (hereinafter to be referred as Fr.1, Fr.2, Fr.3, Fr.4).Getting Fr.2(is second largest part) separate with solvent system execution silicagel column (silica gel) chromatography of chloroform-methanol-water, promptly obtain the highly finished product of Ds-t.Get the second largest part of Fr.3() be that solvent is implemented ODS silica gel (Bondapak) column chromatography and separated with methanol-water, can obtain the highly finished product of Ds-a.They can be added various liquid or solid vehicle subsequently makes tablet, emulsion, capsule and uses for the clinical prevention urinary calculus.Use this type of through refining Longhairy Antenoron Herb medicinal tablets of purifying etc., method was both easy, drug effect concentration height, do not have the effect of paying, and also can solve at present the whole bag of tricks rubble both at home and abroad and the residual stones that causes and the serious problems of recurrence.Also can avoid the effective ingredient wild effect that caused with the crude drug Longhairy Antenoron Herb.
Embodiment of the invention accompanying drawings is as follows:
Accompanying drawing is a Longhairy Antenoron Herb master composition extraction method synoptic diagram:
Get 1 kilogram of the herb of Longhairy Antenoron Herb, also stream was extracted out 8 hours in 8 liters of 80 ℃ of methyl alcohol, Extract filters and gets 50 gram Longhairy Antenoron Herb methyl alcohol fluid extracts after back concentrating under reduced pressure (above is synoptic diagram A) concentrates, again this fluid extract is added equivalent n-butanols-water and do to distribute (this joint is seen as B) for 1 liter, the result obtains water layer portion medicinal extract 14 grams (this joint is seen as C), butanols layer medicinal extract 36 restrains, and it is separated (this joint is seen as D) one-tenth four major parts (being Fr, 1-4) with crosslinked dextran column chromatography.Get 21.6 grams of Fr.2 and use silica gel column chromatographies, use chloroform: methyl alcohol: the ratio of water=9: 2: 0.1～8: 2: 0.2～7: 3: 0.5 launches solvent system (this joint is seen as E), obtain 1.58 restrains Ds-t.Get 9.73 grams of Fr.3 and use the ODS silica gel column chromatography, use the per-cent of 30% methanol-water～40% methanol-water to launch solvent system (this joint is seen as G), can obtain 3.37 gram Ds-a.
Claims (7)
1, a kind of extracting method of urinary calculus control medicine effective ingredient.It is characterized in that also flowing at rudimentary methyl alcohol, drawing Longhairy Antenoron Herb methyl alcohol fluid extract after the filtration, concentrating under reduced pressure (A) and distribute (B) with equivalent n-fourth alcohol and water with Longhairy Antenoron Herb.Get butanols laminar flow medicinal extract and separate (D) with glue connection dextran post layer chromatography.Be divided into four major parts (Fr.1～4), get Fr.2 and use the solvent system of chloroform-methanol-water to implement silica gel column chromatography separation (E), get the Ds-t highly finished product, getting Fr.3 is that solvent execution ODS silica gel column chromatography separates (G) with methanol-water, obtains the Ds-a highly finished product.
2, by the extracting method of the described urinary calculus control of claim 1 medicine effective ingredient, it is characterized in that Longhairy Antenoron Herb methyl alcohol fluid extract equivalent n-butanols-water dispenser method.
3,, it is characterized in that separating the method for Longhairy Antenoron Herb butanols laminar flow medicinal extract with the sephadex chromatography by the extracting method of the described urinary calculus control of claim 1 medicine effective ingredient.
4, by the extracting method of the described urinary calculus control of claim 1 medicine effective ingredient, it is characterized in that the Fr.2 silica gel column chromatography.
5, by the extracting method of the described urinary calculus control of claim 1 medicine effective ingredient, it is characterized in that the ratio that the used chloroform-methanol of Fr.2-water-soluble matchmaker is is 9: 2: 0.1～8: 2: 0.2～7: 3: 0.5.
6, by the extracting method of the described urinary calculus control of claim 1 medicine effective ingredient, it is characterized in that Fr.3 adopts the ODS silica gel column chromatography.
7, by the extracting method of the described urinary calculus control of claim 1 medicine effective ingredient, it is characterized in that the employed methanol-water solvent of Fr.3 is that per-cent is 30%～40%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 88107721 CN1042544A (en) | 1988-11-08 | 1988-11-08 | The extraction method of the principal constituent of Longhairy Antenoron Herb (the pure and mild flavonoid glycoside of triterpene Chinese honey locust) |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 88107721 CN1042544A (en) | 1988-11-08 | 1988-11-08 | The extraction method of the principal constituent of Longhairy Antenoron Herb (the pure and mild flavonoid glycoside of triterpene Chinese honey locust) |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1042544A true CN1042544A (en) | 1990-05-30 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 88107721 Pending CN1042544A (en) | 1988-11-08 | 1988-11-08 | The extraction method of the principal constituent of Longhairy Antenoron Herb (the pure and mild flavonoid glycoside of triterpene Chinese honey locust) |
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| CN (1) | CN1042544A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1044961C (en) * | 1993-03-27 | 1999-09-08 | 河北省科学院生物研究所 | Growth accelerating agent for plant |
| CN101352481B (en) * | 2008-09-05 | 2011-03-02 | 浙江省医学科学院 | Method for preparing Chinese medicine desmodium anti-calculi fractionated extracts and uses thereof |
| EP3078380A4 (en) * | 2013-12-05 | 2017-05-24 | Wuhan Optics Valley Humanwell Bio-pharmaceutical Co. Ltd. | Desmodium styracifolium (osb.) merr. flavonoids capsule, method of preparing same, and application thereof |
-
1988
- 1988-11-08 CN CN 88107721 patent/CN1042544A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1044961C (en) * | 1993-03-27 | 1999-09-08 | 河北省科学院生物研究所 | Growth accelerating agent for plant |
| CN101352481B (en) * | 2008-09-05 | 2011-03-02 | 浙江省医学科学院 | Method for preparing Chinese medicine desmodium anti-calculi fractionated extracts and uses thereof |
| EP3078380A4 (en) * | 2013-12-05 | 2017-05-24 | Wuhan Optics Valley Humanwell Bio-pharmaceutical Co. Ltd. | Desmodium styracifolium (osb.) merr. flavonoids capsule, method of preparing same, and application thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| CI01 | Correction of invention patent gazette |
Correction item: Applicant Correct: Wang Zhirou False: Wang Ganrou Number: 22 Volume: 6 Correction item: Address Correct: Affiliated Hospital of Guangxi Medical College False: Affiliated Hospital of the Guangxi Zhuang Autonomous Region Medical College Number: 22 Volume: 6 |
|
| ERR | Gazette correction |
Free format text: CORRECT: APPLICANT; FROM: WANG GANROU TO: WANG ZHIROU; ADDRESS; FROM: GUANGXI ZHUANG AUTONOMOUS REGION MEDICINE COLLEGE AFFILIATED HOSPITAL TO: GUANGXI AFFILIATED HOSPITAL OF MEDICAL COLLEGE |
|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C01 | Deemed withdrawal of patent application (patent law 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |