CN104252143A - Liquid drop or liquid flow sorting control system and control method thereof - Google Patents
Liquid drop or liquid flow sorting control system and control method thereof Download PDFInfo
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- CN104252143A CN104252143A CN201310259981.7A CN201310259981A CN104252143A CN 104252143 A CN104252143 A CN 104252143A CN 201310259981 A CN201310259981 A CN 201310259981A CN 104252143 A CN104252143 A CN 104252143A
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Abstract
The invention relates to a liquid drop or liquid flow sorting control system comprising a master control portion, a rectification transformation portion, an inversion portion, a signal feedback portion, and ports. The inversion portion, the signal feedback portion, and the ports are respectively and electrically connected with the master control portion; the rectification transformation portion, the inversion portion, and the signal feedback portion are respectively and electrically connected with ports directly or indirectly by a filtering circuit; the rectification transformation portion and the inversion portion are connected electrically and directly or indirectly by the filtering circuit; the rectification transformation portion and the master control portion can be electrically connected or connected in a non-electric mode. Controlled objects include a micro-fluidic chip and other instruments, equipment, devices, chips or components that can accommodate liquid drops or liquid flows. According to the sorting control system, on the basis of controlling on/off of an electric signal of an electrode of an object, real-time rapid controlling of the liquid drop or liquid flow in the controlled object is realized.
Description
Technical field
The present invention relates to the Grading System of a kind of drop or liquid stream.
Background technology
Now, a lot of fields in national economy, as drug research and development, medical science detection, food development, living things catalysis, chemical materials purification, DNA sequencing etc., all relate to the screening link of sample, the efficiency of this link directly affects R&D cycle and the production cycle of whole product.Along with the progress of science and technology and the development in epoch, the sample preparation in a lot of field, screening, reaction and related experiment can realize on the chip of several square centimeters even less, the microflow control technique of one of seven kinds of technology that Here it is is called as " the change world ", microflow control technique possesses the ability on the chip of a biological or chemical laboratory simmer down to several square centimeters even less, is countries in the world scientific worker favor " forward position " in sciemtifec and technical sphere.
Now, still classic method is adopted at the sample sifter ring selection joint of a lot of research and development, production field, comprise use liquid getting device, get the instruments such as liquid rifle, kit, inefficiency and waste is serious, the aspect relevant cost such as reagent, manpower, management is remained high, a lot of developed country employs robot controlling, screening effeciency is made to obtain raising to a certain extent, but high fringe cost makes it promote runs into bottleneck, and, the waste of sample in screening process does not significantly reduce, and screening effeciency yet improves a lot space.
Microflow control technique is mainly also in development current, relatively limited in application, and the micro-fluidic area research person in the whole world settles one by one to have a lot of technical barrier to wait for, also has some existing technical schemes to be left to be desired and improvement.
The Grading System of drop of the present invention or liquid stream, the Based Intelligent Control of microcontroller or microprocessor is introduced in the experimentation of drop or liquid stream, and by the effect in electric field, magnetic field, realize the high speed screening of fine droplet, the volume of drop can be low to moderate skin upgrading, and relative to screening mode that is artificial and robot, it makes drug research and development, medical science detect, cost that is biological and chemical experiment significantly reduces, efficiency significantly improves, and has convenience concurrently.
Flow cytometer is a kind of maturation, the efficient sorting instrument for cell, its limitation for controlled target must be the cell in continuous flow, if the metabolin of sample is not together with cell " bonding ", so cannot carries out screening according to the speciality of the metabolin of sample and evaluate; Other is as the experiment carrying out with drop type microreactor studying for needs such as virus, bacterium, enzyme, albumen, flow cytometer is all not competent, and the present invention is especially applicable to using drop type microreactor as screening object, the volume of drop can be low to moderate skin upgrading, be 1/10000000th of tradition screening test reagent consumption, and breakneck acceleration reach more than 1,000 times of most significant end robot manipulation speed in the world today.
The present invention is a kind of revolutionary product, has extraordinary application prospect and market expectations.
Summary of the invention
The present invention relates to Grading System and the control method thereof of a kind of drop or liquid stream.The Grading System of drop of the present invention or liquid stream is made up of main control part, rectifier transformer part, Converting Unit, signal feedback part, port etc., and concrete electric connecting mode comprises:
1. Converting Unit, signal feedback part, port are electrically connected with main control part respectively;
2. rectifier transformer part, Converting Unit, signal feedback part are directly electrically connected with port respectively or are indirectly electrically connected through filtering circuit;
3. rectifier transformer part and Converting Unit are directly electrically connected or are indirectly electrically connected through filtering circuit;
4., between rectifier transformer part and main control part, when rectifier transformer part adopts " first through transformer or pressure regulator transformation, then through diode rectification " scheme without electrical connection, other scheme is directly electrically connected or is indirectly electrically connected through filtering circuit.
In the Grading System of the drop that the present invention relates to or liquid stream, main control part comprise in microprocessor, microcontroller one or more, wherein the type of microcontroller includes but not limited to single-chip microcomputer, DSP, CPLD, FPGA, PLC, main control part is the control axis of whole system, is responsible for the signals collecting of system, data processing, real-time analysis and high-speed decision.
In the Grading System of the drop that the present invention relates to or liquid stream, the circuit of Converting Unit has two kinds of constituted modes, and one comprises several converter bridge arm, and another kind comprises several integrated power modules.
Situation for comprising converter bridge arm: each brachium pontis is in series by multiple full control switching device, each full control switching device inverse parallel fly-wheel diode.This controls the electronic power switch device that switching device refers to can control to open and can control to turn off entirely, and type includes but not limited to transistor, IGBT, MOSFET, IGCT, IEGT, GTO, SET.
Situation for comprising integrated power module: the type of integrated power module includes but not limited to IPM power model.
The control object of the Grading System of the drop that the present invention relates to or liquid stream comprises: micro-fluidic chip and other can hold the instrument of drop or liquid stream, unit, chip or parts.Control object has following feature:
1. there is the passage flowed for liquid;
2. passage proximate has several electrodes.
For the control object possessing above-mentioned feature, the Grading System of drop of the present invention or liquid stream, by being switched on or switched off specific electric signal respectively on Different electrodes, and then realizes the real-time control to drop in control object passage or liquid stream.
The Grading System of drop of the present invention or liquid stream has the control program of following plurality of optional:
1. system comprises rectifier transformer part, system rectifier transformer part is directly electrically connected with Converting Unit, port respectively or is indirectly electrically connected through filtering circuit, corresponding control method is: the civil power accessed by port is first through transformer or pressure regulator transformation, use diode rectification again, finally generated the electric signal of special parameter and feature by Converting Unit, be connected to port by lead-in wire.
2. system comprises rectifier transformer part, rectifier transformer part and respectively main control part, Converting Unit, port are directly electrically connected or are indirectly electrically connected through filtering circuit, and corresponding control method is: the civil power accessed by port is first with the circuit rectifies be made up of diode or thyristor
,re-use DC chopper circuit transformation, finally generated the electric signal of special parameter and feature by Converting Unit, be connected to port by lead-in wire.
3. system comprises rectifier transformer part, rectifier transformer part respectively with main control part, Converting Unit, port is directly electrically connected or is indirectly electrically connected through filtering circuit, corresponding control method is: the civil power accessed by port first realizes rectification and transformation through PWM rectification circuit simultaneously, the electric signal of special parameter and feature is finally generated by Converting Unit, port is connected to by lead-in wire, PWM rectification circuit is except unsteady flow main circuit, also comprise some inductance or reactor, the constituted mode that wherein unsteady flow main circuit can be selected includes but not limited to: comprise several converter bridge arm or integrated power module, for the situation comprising converter bridge arm, each brachium pontis is in series by multiple full control switching device, each full control switching device inverse parallel fly-wheel diode, here full control switching device refers to the electronic power switch device that can control to open and can control to turn off, type includes but not limited to transistor, IGBT, MOSFET, IGCT, IEGT, GTO, SET, for the situation comprising integrated power module, the type of integrated power module includes but not limited to IPM power model.
4. system comprises rectifier transformer part, rectifier transformer part is directly electrically connected with main control part, Converting Unit, port respectively or is indirectly electrically connected through filtering circuit, corresponding control method is: the civil power accessed by port first carries out uncontrollable rectifier with diode, then through Converting Unit high-frequency inversion, adopt high-frequency transformer transformation afterwards, in addition after, last high-frequency rectification, is finally generated the electric signal of special parameter and feature by another Converting Unit, is connected to port by lead-in wire.
In the Grading System of drop of the present invention or liquid stream, the object of signal feedback part collection includes but not limited to:
1. the light signal of drop or liquid stream, electric signal, magnetic signal and other type signal in control object, the electric signal collected is directly or indirectly send to main control part after the links such as amplification filtering; The light signal collected is converted into electric signal more directly or indirectly send to main control part after the links such as amplification filtering.
2. some node locations that in system, electric signal changes, these node locations include but not limited to: rectification input side, rectification outgoing side, inversion input side, inversion outgoing side, direct current transformation input side, direct current transformation outgoing side, feedback circuit input side, system can according to actual conditions select in these nodes part or all.
3. mains input side.
In the Grading System of drop of the present invention or liquid stream, have some filtering circuits, the position that filtering circuit can be selected includes but not limited to: mains input side, rectification input and outgoing side, inversion input and outgoing side, direct current transformation input and outgoing side, feedback circuit input side, port.
Filtering circuit carries out filtering to the voltage of the links of electric signal transmission in system, current harmonics, and has the effect of burning voltage, electric current concurrently.
The parameter area of the electric signal that the Grading System of drop of the present invention or liquid stream exports through port is as follows: voltage magnitude between 3 volts to 5 kilovolts or negative 5 kilovolts between negative 3 volts, electric signal is that direct current or frequency are between 50 hertz to 1 Gigahertz.
The Grading System of drop of the present invention or liquid stream is except exporting DC signal as required, also exportable non-dc electric signal, the type of non-dc electric signal includes but not limited to: the result of the functional operation of the distortion of square wave, triangular wave, sine wave, sawtooth wave, integration ripple, differential ripple, staircase waveform, forementioned waveform, the superposition of aforementioned multiple waveforms, aforementioned multiple waveforms.
In the Grading System of drop of the present invention or liquid stream, the selection gist of electric signal parameter includes but not limited to:
1. the distance of drop and electrode in the passage of control object;
2. the material of control object;
3. the parameter of drop or liquid stream in control object;
4. the characteristic of the electrode of control object.
Accompanying drawing explanation
Fig. 1 is the Control system architecture schematic diagram of the embodiment of the present invention.
Fig. 2 is the main control part circuit theory diagrams of the embodiment of the present invention.
Fig. 3 is the rectifier transformer partial schematic diagram of the embodiment of the present invention.
Fig. 4 is the filtering circuit schematic diagram of the embodiment of the present invention.
Fig. 5 is the Converting Unit schematic diagram of the embodiment of the present invention.
Embodiment
Following examples belong to the one in concrete form of the present invention, and the object provided is more detailed description the present invention, instead of limits the scope of the invention, and neither limit application form of the present invention.
The Control system architecture schematic diagram of the embodiment of the present invention as shown in Figure 1, comprises main control part, rectifier transformer part, Converting Unit, signal feedback part, filtering circuit etc.
As shown in Figure 2, U1 is microcontroller to the main control part circuit theory diagrams of the embodiment of the present invention, accurately controls the operation of system, and carries out real-time analysis and decision-making to sensing data; U17 is for microcontroller provides the crystal oscillator of accurate clock; U11 is the storer for storing data; Main control part carries out message exchange with external function module respectively by ports such as J11, J12, J13, J15, J20, J21, J31, J32, and wherein J11 is On-off signal port, the various switching value feedback informations that acceptable system sends; J12 is output switch parameter interface, and the digital output modul information of main control part sends through this port; J13 is serial communication interface, the feedback information that acceptable system various piece sends; J15 is microcontroller programming interface; J21 is the data/address bus interface for parallel communications; J31 is SPI communication interface; J32 is SCI communication interface; Microcontroller accurately calculates real time data, and can export control signal accurately respectively to rectifier transformer part, Converting Unit through J20 interface.
The rectifier transformer partial schematic diagram of the embodiment of the present invention as shown in Figure 3, select the topological structure of two level PWM rectification circuits, transformation is realized while rectification, PWM rectification circuit is except comprising unsteady flow main circuit, also comprise inductance L s, its unsteady flow main circuit comprises 2 converter bridge arm, and each brachium pontis is in series by 2 IGBT, each IGBT inverse parallel fly-wheel diode.
The filtering circuit schematic diagram of the embodiment of the present invention as shown in Figure 4, adopts L-type filtering mode, is made up of filter capacitor Cd21 and filter inductance Ld21, and the harmonic wave that can be mingled with in transmitting procedure electric signal carries out filtering or reduces the content of harmonic wave.
The Converting Unit schematic diagram of the embodiment of the present invention as shown in Figure 5, this inverter circuit adopts the topological structure of two level, containing two identical converter bridge arm of structure, each converter bridge arm is in series by the IGBT that two groups are parallel with fly-wheel diode, and inverter circuit DC side parallel has Support Capacitor C31.
The control method of the present embodiment is: rectifier transformer part selects the topological structure of PWM rectification circuit, transformation is realized while rectification, main control part is through the information of the state of signal feedback part real-time reception rectifier transformer part, Converting Unit and micro-fluidic chip, and high-speed decision, send real-time control signal respectively to rectifier transformer part, Converting Unit and micro-fluidic chip.The present embodiment to the parameter of the electric signal that the electrode of micro-fluidic chip exports is: voltage magnitude is 300 volts, signal frequency is 1kHz, and the type of electric signal is square wave.
The present embodiment introduces the Based Intelligent Control of microcontroller in micro-fluidic experiment flow, and by the effect of electric field, realize the high speed screening of micro-fluidic chip endothelium upgrading fine droplet, relative to screening mode that is artificial and robot, it makes drug research and development, medical science detect, cost that is biological and chemical experiment significantly reduces, and efficiency significantly improves.
The present invention has higher promotional value.
Claims (10)
1. the Grading System of drop or liquid stream and a control method thereof, it is characterized in that comprising main control part, Converting Unit, port, wherein Converting Unit is directly electrically connected with main control part, port respectively or is indirectly electrically connected through filtering circuit.
2. the Grading System of drop according to claim 1 or liquid stream, it is characterized in that main control part comprises in microprocessor, microcontroller one or more, wherein the type of microcontroller includes but not limited to single-chip microcomputer, DSP, CPLD, FPGA, PLC.
3. the Grading System of drop according to claim 2 or liquid stream, it is characterized in that the constituted mode that the circuit of Converting Unit can be selected includes but not limited to: comprise several converter bridge arm or integrated power module, for the situation comprising converter bridge arm, each brachium pontis is in series by multiple full control switching device, each full control switching device inverse parallel fly-wheel diode, here full control switching device refers to the electronic power switch device that can control to open and can control to turn off, type includes but not limited to transistor, IGBT, MOSFET, IGCT, IEGT, GTO, SET, for the situation comprising integrated power module, the type of integrated power module includes but not limited to IPM power model.
4. the Grading System of drop according to claim 3 or liquid stream, it is characterized in that control object comprises micro-fluidic chip and other can hold the instrument of drop or liquid stream, unit, chip or parts, control object has following feature: have the passage flowed for liquid; Passage proximate has several electrodes, by being switched on or switched off specific electric signal on Different electrodes, realizes the real-time control to drop in control object or liquid stream.
5. the Grading System of drop according to claim 4 or liquid stream, it is characterized in that comprising rectifier transformer part, rectifier transformer part is directly electrically connected with Converting Unit, port respectively or is indirectly electrically connected through filtering circuit, corresponding control method is: the civil power accessed by port is first through transformer or pressure regulator transformation, use diode rectification again, finally generated the electric signal of special parameter and feature by Converting Unit, be connected to port by lead-in wire.
6. the Grading System of drop according to claim 4 or liquid stream, it is characterized in that comprising rectifier transformer part, rectifier transformer part is directly electrically connected with Converting Unit, port respectively or is indirectly electrically connected through filtering circuit, and corresponding control method is: the civil power accessed by port is first with the circuit rectifies be made up of diode or thyristor
,re-use DC chopper circuit transformation, finally generated the electric signal of special parameter and feature by Converting Unit, be connected to port by lead-in wire.
7. the Grading System of drop according to claim 4 or liquid stream, it is characterized in that comprising rectifier transformer part, rectifier transformer part respectively with Converting Unit, port is directly electrically connected or is indirectly electrically connected through filtering circuit, corresponding control method is: the civil power accessed by port first realizes rectification and transformation through PWM rectification circuit simultaneously, the electric signal of special parameter and feature is finally generated by Converting Unit, port is connected to by lead-in wire, PWM rectification circuit is except unsteady flow main circuit, also comprise some inductance or reactor, the constituted mode that wherein unsteady flow main circuit can be selected includes but not limited to: comprise several converter bridge arm or integrated power module, for the situation comprising converter bridge arm, each brachium pontis is in series by multiple full control switching device, each full control switching device inverse parallel fly-wheel diode, here full control switching device refers to the electronic power switch device that can control to open and can control to turn off, type includes but not limited to transistor, IGBT, MOSFET, IGCT, IEGT, GTO, SET, for the situation comprising integrated power module, the type of integrated power module includes but not limited to IPM power model.
8. the Grading System of drop according to claim 4 or liquid stream, it is characterized in that comprising rectifier transformer part, rectifier transformer part is directly electrically connected with Converting Unit, port respectively or is indirectly electrically connected through filtering circuit, corresponding control method is: the civil power accessed by port first carries out uncontrollable rectifier with diode, then through Converting Unit high-frequency inversion, adopt high-frequency transformer transformation afterwards, in addition last high-frequency rectification after, finally generated the electric signal of special parameter and feature by another Converting Unit, be connected to port by lead-in wire.
9. according to claim 1 to the Grading System of drop according to claim 8 or liquid stream, it is characterized in that comprising signal feedback part, signal feedback part respectively with main control part, port is directly electrically connected or is indirectly electrically connected through filtering circuit, also can select only directly to be electrically connected with main control part or to be indirectly electrically connected through filtering circuit, signal or the signal that caused by control object in signal feedback part acquisition system, the kind of this signal includes but not limited to: light signal, electric signal, magnetic signal, one or more feedback signals collected are direct or indirectly send to main control part after the links such as filtering.
10. according to claim 1 to the Grading System of drop according to claim 8 or liquid stream, it is characterized in that there is some filtering circuits, and system sends the electric signal of special parameter and feature through port, the position of filtering circuit includes but not limited to: mains input side, rectification outgoing side, inversion outgoing side, direct current transformation outgoing side, feedback circuit input side, port, filtering circuit carries out filtering to the harmonic wave of the links of electric signal transmission in system, and has pressure stabilization function concurrently, and the parameter of the electric signal that system sends through port is: voltage magnitude is between 3V to 5000V or between-5000V to-3V, electric signal be direct current or frequency between 50Hz to 1GHz, wherein, the type of non-straight flow pattern electric signal includes but not limited to: square wave, triangular wave, sinusoidal wave, sawtooth wave, integration ripple, differential ripple, staircase waveform, the distortion of forementioned waveform, the superposition of aforementioned multiple waveforms, the result of the functional operation of aforementioned multiple waveforms, the selection gist of electric signal parameter includes but not limited to: the distance of drop and electrode in the passage of control object, the material of control object, the parameter of drop or liquid stream in control object, the characteristic of the electrode of control object.
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| CN201310259981.7A CN104252143A (en) | 2013-06-27 | 2013-06-27 | Liquid drop or liquid flow sorting control system and control method thereof |
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| CN201310259981.7A CN104252143A (en) | 2013-06-27 | 2013-06-27 | Liquid drop or liquid flow sorting control system and control method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106597983A (en) * | 2016-12-30 | 2017-04-26 | 昆明昆船逻根机场物流系统有限公司 | Conveying line goods drop detection system |
| CN107153122A (en) * | 2017-05-27 | 2017-09-12 | 哈尔滨工业大学（威海） | Digital microcurrent-controlled biochip in situ rest structure and method based on serial communication |
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Application publication date: 20141231 |