CN104247663A - Method for rapidly propagating embelia ribes through tissue culture - Google Patents
Method for rapidly propagating embelia ribes through tissue culture Download PDFInfo
- Publication number
- CN104247663A CN104247663A CN201410540390.1A CN201410540390A CN104247663A CN 104247663 A CN104247663 A CN 104247663A CN 201410540390 A CN201410540390 A CN 201410540390A CN 104247663 A CN104247663 A CN 104247663A
- Authority
- CN
- China
- Prior art keywords
- illumination
- vain
- stem section
- agar
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention researches a method for rapidly propagating embelia ribes through tissue culture. The method comprises the following steps: sterilizing stem segments, inducing axillary buds, propagating cluster buds, inducing rooting, hardening seedlings, transplanting and the like. The method aims to discover a way of establishing a high-efficiency in-vitro embelia ribes regenerating system by a tissue culture technique, achieve rapid propagation of embelia ribes, solve the problems of shortage of seedlings and the like, provide a technical support for factory production of the embelia ribes, and lay a technical foundation for realization of the value of the embelia ribes, storage of germplasm, transgenic research and the like.
Description
Technical field
The present invention relates to the tissue cultures spending common embelia fruit in vain, belong to biological technical field.
Background technology
Spend common embelia fruit in vain,
embelia ribes, Myrsinacea, has another name called: embelin, plan currant, the public plate of sheep are young, undershrub, high 90-120 centimetre, branch, without hair, Cheng Manzhuan sometimes, leaf is avette to oblong, long 5-7.5 centimetre, wide 2-2.5 centimetre, tip is gradually sharp, basal circular, Quan Yuan, without hair, below evergreen band white; The long 5-7 millimeter of petiole, has straight hem edge.The raw top armpit of holding concurrently in panicle top is raw, close by pubescence; Bud is little; Spend minimum, polygamy, long 3 millimeters of bennet; Calyx is little, 5 drastic cracks, sharp head; Petal 5, white, is about 2 millimeters, has echinid; Stamen 5, filigree and is born in petal, medicine ovum shape oblong; Ovary is upper, in avette in female flower, in male flower, deteriorates to taper shape, style is cylindrical, column cap head, and berry is spherical, seed is spherical, and base portion is porose, January at florescence, the distribution ground such as Guangdong, Guangxi, fruit, containing 2.45% anthracene shellfish element, contains vitamin again, its dry fruit kills tapeworm effect, active ingredient is anthracene shellfish element, is used as contraceptive, can suppresses endometrial alkaline phosphatase activities in India.
Summary of the invention
Technical problem to be solved by this invention spends the propagation method of common embelia fruit tissue cultures in vain, the inventive method utilizes tissue culture technique to explore the approach set up and spend common embelia fruit high-efficiency in-vitro regenerating system in vain, realize the Fast-propagation spending common embelia fruit in vain, solve the problems such as seedling shortage, for the factorial praluction spending common embelia fruit in vain provides technical support, for spending common embelia fruit value realization in vain, plant the preservation, genetically modified research etc. of matter and establish technical foundation.
For solving the problems of the technologies described above, the present invention adopts following technical proposal:
The plant of growth selection stalwartness, cut the explant stem section of about 5cm, every 3 buds are cut into one section, soak 8min with suds, running water 20min, be soaked in 1g/L carbendazim+1g/L tpn+1g/L to kill virus in vanadium solution, be placed in 2.5h that 130r/min shaking table vibrates, rinse well, 70% ethanol postincubation 25s on superclean bench, 1g/L mercuric chloride process 6min, aseptic water washing is clean, and the aseptic stem section of disinfecting is inoculated into Ne+6-BA5.0mg/L+GA
3the induction of axillalry bud is carried out in the medium of 0.5mg/L, additional saccharose 30g/L, agar 6.5g/L, cultivation temperature 25 DEG C, intensity of illumination 1800lx, illumination 10h, the bud seedling derived grows to about 3cm and accesses Ne+2, 4-D1.5-2.0mg/L carry out the propagation of Multiple Buds in medium, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 4500lx, photophase 12h/d, temperature 25 DEG C, cultivate in Multiple Buds access MS+IBA0.5-0.6mg/L+IAA0.5-0.6mg/L medium out and carry out root induction, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 6500lx, photophase 16h/d, temperature 25 DEG C.
Adopt the present invention to prepare to spend common embelia fruit rooting rate in vain high, availability is large, is beneficial to implant mass.
Below in conjunction with embodiment, the present invention is further elaborated, but the scope of protection of present invention is not limited to following embodiments.
Embodiment
Embodiment 1
The plant of growth selection stalwartness, cut the explant stem section of about 5cm, every 3 buds are cut into one section, soak 8min with suds, running water 20min, be soaked in 1g/L carbendazim+1g/L tpn+1g/L to kill virus in vanadium solution, be placed in 2.5h that 130r/min shaking table vibrates, rinse well, 70% ethanol postincubation 25s on superclean bench, 1g/L mercuric chloride process 6min, aseptic water washing is clean, and the aseptic stem section of disinfecting is inoculated into Ne+6-BA5.0mg/L+GA
3the induction of axillalry bud is carried out in the medium of 0.5mg/L, additional saccharose 30g/L, agar 6.5g/L, cultivation temperature 25 DEG C, intensity of illumination 1800lx, illumination 10h, the bud seedling derived grows to about 3cm and accesses Ne+2, the propagation of Multiple Buds is carried out in 4-D1.5mg/L medium, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 4500lx, photophase 12h/d, temperature 25 DEG C, cultivate in Multiple Buds access MS+IBA0.5mg/L+IAA0.5mg/L medium out and carry out root induction, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 6500lx, photophase 16h/d, temperature 25 DEG C, rooting rate 92%.
Embodiment 2
The plant of growth selection stalwartness, cut the explant stem section of about 5cm, every 3 buds are cut into one section, soak 8min with suds, running water 20min, be soaked in 1g/L carbendazim+1g/L tpn+1g/L to kill virus in vanadium solution, be placed in 2.5h that 130r/min shaking table vibrates, rinse well, 70% ethanol postincubation 25s on superclean bench, 1g/L mercuric chloride process 6min, aseptic water washing is clean, and the aseptic stem section of disinfecting is inoculated into Ne+6-BA5.0mg/L+GA
3the induction of axillalry bud is carried out in the medium of 0.5mg/L, additional saccharose 30g/L, agar 6.5g/L, cultivation temperature 25 DEG C, intensity of illumination 1800lx, illumination 10h, the bud seedling derived grows to about 3cm and accesses Ne+2, the propagation of Multiple Buds is carried out in 4-D2.0mg/L medium, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 4500lx, photophase 12h/d, temperature 25 DEG C, cultivate in Multiple Buds access MS+IBA0.6mg/L+IAA0.6mg/L medium out and carry out root induction, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 6500lx, photophase 16h/d, temperature 25 DEG C, rooting rate 93%.
Embodiment 3
The plant of growth selection stalwartness, cut the explant stem section of about 5cm, every 3 buds are cut into one section, soak 8min with suds, running water 20min, be soaked in 1g/L carbendazim+1g/L tpn+1g/L to kill virus in vanadium solution, be placed in 2.5h that 130r/min shaking table vibrates, rinse well, 70% ethanol postincubation 25s on superclean bench, 1g/L mercuric chloride process 6min, aseptic water washing is clean, and the aseptic stem section of disinfecting is inoculated into Ne+6-BA5.0mg/L+GA
3the induction of axillalry bud is carried out in the medium of 0.5mg/L, additional saccharose 30g/L, agar 6.5g/L, cultivation temperature 25 DEG C, intensity of illumination 1800lx, illumination 10h, the bud seedling derived grows to about 3cm and accesses Ne+2, the propagation of Multiple Buds is carried out in 4-D2.0mg/L medium, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 4500lx, photophase 12h/d, temperature 25 DEG C, cultivate in Multiple Buds access MS+IBA0.5mg/L+IAA0.5mg/L medium out and carry out root induction, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 6500lx, photophase 16h/d, temperature 25 DEG C, rooting rate 94%.
Claims (2)
1. spend a method for quickly breeding for common embelia fruit tissue cultures in vain, comprise the sterilization of stem section, the induction of axillalry bud, the propagation of Multiple Buds, root induction and acclimatization and transplants etc., its key step is as follows:
(1) the stem section spending common embelia fruit in vain is got, disinfection;
(2) the aseptic stem section that step (1) was disinfected is inoculated into Ne+6-BA5.0mg/L+GA
3the induction of axillalry bud is carried out, additional saccharose 30g/L, agar 6.5g/L, cultivation temperature 25 DEG C, intensity of illumination 1800lx, illumination 10h in the medium of 0.5mg/L;
(3) get bud seedling that step (2) derives to grow to about 3cm and access Ne+2, carry out the propagation of Multiple Buds in 4-D1.5-2.0mg/L medium, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 4500lx, photophase 12h/d, temperature 25 DEG C;
(4) step (3) is cultivated in Multiple Buds access MS+IBA0.5-0.6mg/L+IAA0.5-0.6mg/L medium out and carry out root induction, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 6500lx, photophase 16h/d, temperature 25 DEG C.
2. according to a kind of method for quickly breeding spending common embelia fruit tissue cultures in vain according to claim 1, it is characterized in that: the acquisition of spending the aseptic stem section of common embelia fruit described in step (1) in vain is the plant of growth selection stalwartness, cut the explant stem section of about 5cm, every 3 buds are cut into one section, 8min is soaked with suds, running water 20min, being soaked in 1g/L carbendazim+1g/L tpn+1g/L kills virus in vanadium solution, be placed in 2.5h that 130r/min shaking table vibrates, rinse well, 70% ethanol postincubation 25s on superclean bench, 1g/L mercuric chloride process 6min, aseptic water washing is clean.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410540390.1A CN104247663A (en) | 2014-10-14 | 2014-10-14 | Method for rapidly propagating embelia ribes through tissue culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410540390.1A CN104247663A (en) | 2014-10-14 | 2014-10-14 | Method for rapidly propagating embelia ribes through tissue culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104247663A true CN104247663A (en) | 2014-12-31 |
Family
ID=52183171
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410540390.1A Pending CN104247663A (en) | 2014-10-14 | 2014-10-14 | Method for rapidly propagating embelia ribes through tissue culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104247663A (en) |
-
2014
- 2014-10-14 CN CN201410540390.1A patent/CN104247663A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104082138A (en) | Tissue-culture rapid propagation method of Aristolochia fordiana Hemsl | |
| CN105961197B (en) | A kind of Moringa highly efficient regeneration method | |
| CN106417011A (en) | Wild bletilla striata tissue culture rapid propagation method | |
| CN102499088A (en) | Method for quickly breeding seedlings of Guangxi anoectochilus roxburghii capsules by utilizing Guangxi anoectochilus roxburghii capsules | |
| CN103688865A (en) | Inducing medium and method for improving survival rate of butterfly orchid pedicel | |
| CN109618932A (en) | A kind of method of rhododendron dauricum adventitious bud inducing and plant regeneration | |
| CN104304019A (en) | Intermediate propagation method of hydrangea xanthoneura tissue culture | |
| CN106613960B (en) | A kind of Helen's pocket orchid callus regeneration system rapid propagation method | |
| CN103718969B (en) | A kind of logical cultured in vitro regeneration plant method with a smile of poem beautiful jade | |
| CN107484666A (en) | A kind of tissue cultivation rapid breeding method of the root of herbaceous peony | |
| CN110786157A (en) | Method for promoting rooting of ilex verticillata tissue culture seedlings | |
| CN105830918B (en) | A kind of method for improving serrate clubmoss herb gemma transplanting survival rate | |
| CN106359101A (en) | Tissue culture and rapid propagation method of ficus deltoidea | |
| CN104488722B (en) | A kind of quick breeding method for tissue culture of South America crutch flower | |
| CN105104206A (en) | In-vitro conservation method for liparis nervosa | |
| CN102301958B (en) | Method for culturing in vitro tissues of Semiliquidambar cathayensis | |
| CN103477988A (en) | Culture in vitro and rapid propagation method for syzygium grijsii | |
| CN107410033B (en) | The rapid propagation method of national spice berry snippings | |
| CN107667865B (en) | A kind of Ming River ceratostigma plumbaginoides Bunge subculture quick-breeding method | |
| CN103975853B (en) | The lucky method for tissue culture of a kind of colored thick rib grass | |
| CN100391333C (en) | Aseptic seedling tissue culturing and test tube seedling hardening off and transplating technology for anthurium andraeanum | |
| CN110301278A (en) | One kind " Red Male " Kiwi berry is without Pathogenic Fungus of Canker seedling fostering technology | |
| CN109247147A (en) | A kind of method that tea tree rapid cuttage is taken root | |
| CN104255498A (en) | Rapid seedling propagation method for Chinese aralis | |
| CN104170733B (en) | The method of Flos Lonicerae aseptic tissue cultured seedling petiole callus root induction |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20141231 |
|
| WD01 | Invention patent application deemed withdrawn after publication |