CN104232693A - Method for fermenting galactosyl alga biomass - Google Patents
Method for fermenting galactosyl alga biomass Download PDFInfo
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Abstract
The invention provides a method for fermenting galactosyl alga biomass. According to the method, a thallus which contains a galactose operon is induced and activated by adopting a galactose culture body in the anaerobic fermentation process of the galactosyl alga biomass. An experiment proves that the method provided by the invention can be used for effectively activating the metabolic pathway of galactose, greatly improving the fermentation efficiency of the galactose, efficiently producing the biological fuel or the chemical intermediates, such as alcohol organic substances, namely ethanol, butanol, isobutanol, propanol, 1,3-propanediol and 1,4-propanediol, and alkane organic substances, thereby opening a new way for efficiently utilizing the galactosyl alga biomass.
Description
Technical field
The invention belongs to bioenergy technical field, be specifically related to a kind of galactosyl algae bio matter, namely after degraded, monose material is mainly the fermentation process of the algae bio matter of semi-lactosi.
Background technology
Energy security caused by a large amount of consumption and the amblent air temperature variation issue of fossil oil are day by day serious, therefore, biofuel (such as alcohol fuel, butanols, the isopropylcarbinol etc.) substitute fossil fuels utilizing biology to wait renewable resources to produce becomes research and development focus in recent decades.Such as, current alcohol fuel successfully instead of part oil; But the source of alcohol fuel is mainly corn and sugarcane, so along with the increase day by day to alcohol fuel demand, likely bring the problem such as grain security and ecological damage.Therefore, non-grain ethanol is paid close attention to by domestic and international gradually, and its raw material mainly comprises cellulose substances and seaweeds biomass.
Marine Algae In Photosynthesis efficiency is high, output large, and having the advantage of not striving grain with people and not striving ground with grain, is one of primary study direction of current biofuel or chemical intermediate.Wherein, the large-scale cultivation technology relative maturity of kelp; in Asian countries; red algae and the brown alga output of unit surface reach as high as 70 ~ 80 tons/hectare/years; it is 2 ~ 4 times of land plant output; and its carbohydrate content for the fuel such as fermentative production of ethanol or chemical intermediate enriches, and accounts for 50 ~ 70% of its total biological quality, therefore has good development prospect.
Wherein, red algae output accounts for the half of total marine alga output, but its glucide composition is different from land plant, and the monose after its degraded is mainly semi-lactosi, is namely called galactosyl algae bio.At present, about the research of algae bio matter fermentation process, major part is all for the algae being main polysaccharide with glucose, fructose etc. (being called glucosyl group algae), and is not take semi-lactosi as the algae of main polysaccharide for such as red algae etc.Therefore, utilize existing method ferment red algae biomass time, often there is semi-lactosi nonfermented or fermentation efficiency problem on the low side, limit the fermentation production rate of large-scale red algae biomass to a great extent.
Summary of the invention
Technical purpose of the present invention is for the above-mentioned state of the art, provides a kind of fermentation process of galactosyl algae bio matter, utilizes the method can improve the fermentation efficiency of semi-lactosi, thus improves the output of the tunning such as biofuel or chemical intermediate.
In order to realize above-mentioned technical purpose, the present inventor finds after long-term experiment is explored, semi-lactosi, glucose and the fructose pathways metabolism in anaerobically fermenting is different, the pathways metabolism of semi-lactosi needs to cultivate body induced activation thalline and could start, and the cultivation body adopted in existing thalline induced activation process is unfavorable for even suppressing the thalline containing gal operon to activate, thus greatly reduce the fermentation rate of semi-lactosi.And the present inventor is in the induced activation process of thalline, find after creatively adopting semi-lactosi to cultivate the cultivation body induced activation thalline of body replacement employing at present, the fermentation rate of semi-lactosi increases substantially, thus substantially increases the output of tunning.
Concrete technical scheme of the present invention is: a kind of fermentation process of galactosyl algae bio matter, and the method comprises following process: described algae bio matter degraded, obtain degraded product; Adopt and cultivate body induced activation thalline; Thalline after induced activation is accessed this degraded product and carries out anaerobically fermenting; It is characterized in that: described thalline is the yeast containing gal operon or the intestinal bacteria containing gal operon; In described thalline induced activation process, cultivating body is semi-lactosi nutrient solution or solid semi-lactosi substratum.
After described galactosyl algae bio matter refers to degraded, monose material is mainly the algae bio matter of semi-lactosi, include but not limited to red algae etc., such as the kelp etc. of Gelidium (Gelidium), centipede Trentepohlia (Grateloupia), Eucheuma (Eucheuma) and Gracilaria (Gracilaria).
Described degraded refers to and the polysaccharide fraction in this galactosyl algae bio matter is fully degraded to available monose, and this monose mainly comprises semi-lactosi.This degradation technique is not limit, and comprises mechanical degradation, chemical degradation, biological degradation etc., such as acid hydrolysis, enzyme liberating, sour enzyme liberating, microbiological deterioration etc.
Described degraded product form is not limit, and comprises the forms such as solid, semisolid and liquid.
Described thalline is the yeast containing gal operon or the intestinal bacteria containing gal operon.Wherein, yeast sources form is not limit, and can be all by being separated the yeast obtained, as corrupt substance, soil sample or water sample; It can be the barms of preservation in thalline storehouse; It can be commercial yeast; Also can be through genetic engineering modified yeast and yeast mutant etc.
As preferably, in described thalline induced activation process, thalline is 0.1% grams per milliliter ~ 2% grams per milliliter, more preferably 0.5% grams per milliliter with the mass volume ratio cultivating body.
Described thalline induced activation process can be the activation of standing inducing culture, and the inducing culture that also can vibrate activates.
As preferably, described semi-lactosi is cultivated in body, and semi-lactosi mass percent concentration is 0.5% ~ 5%(w/v).
As preferably, described thalline induced activation process is: added by thalline in semi-lactosi nutrient solution or solid semi-lactosi substratum, and vibrate induced activation 2 hours ~ 24 hours at the temperature of 25 DEG C ~ 40 DEG C, and hunting speed is 0 ~ 300 rpm.
Described anaerobically fermenting comprises intermittent type anaerobically fermenting, continuous anaerobic fermentation or semicontinuous anaerobically fermenting etc.
In described anaerobic fermentation process, as preferably, the thalline after induced activation is 1:1 ~ 1:10 with the volume ratio of degraded mash.
As preferably, described anaerobically fermenting carries out under 25 DEG C ~ 35 DEG C conditions.
As preferably, described anaerobic fermentation process carries out 12 hours ~ 168 hours.
As preferably, with stirring in described anaerobic fermentation process, stirring velocity is 10 revs/min ~ 300 revs/min.
Described anaerobically fermenting product is biofuel or chemical intermediate etc., alcohols organic substance and the alkanes organic substances etc. such as such as ethanol, butanols, isopropylcarbinol, propyl alcohol, 1,3-PD, Isosorbide-5-Nitrae-propylene glycol.
In sum, the present invention is in the anaerobic fermentation process of galactosyl algae bio matter, semi-lactosi cultivation body induced activation is adopted to contain the thalline of gal operon, thus effectively have activated the pathways metabolism of semi-lactosi, increase substantially the fermentation rate of semi-lactosi, thus substantially increase tunning, for efficiency utilization galactosyl algae bio matter obtains biofuel or chemical intermediate, such as ethanol, butanols, isopropylcarbinol, propyl alcohol, 1, ammediol, 1, the alcohols organic substances such as 4-propylene glycol, and alkanes organic substance etc. opens new way.
Accompanying drawing explanation
Fig. 1 is embodiment 1 and gelidium acid hydrolysis products galactose concentration curve over time in anaerobic fermentation process in comparative example 1;
Fig. 2 is embodiment 1 and gelidium acid hydrolysis products alcohol concn curve over time in anaerobically fermenting products therefrom in comparative example 1;
Fig. 3 is that embodiment 2 is schemed over time with Grateloupia filicina (Wulf.) acid hydrolysate galactose content in anaerobic fermentation process in comparative example 2;
Fig. 4 is embodiment 2 and Grateloupia filicina (Wulf.) acid hydrolysate alcohol concn curve over time in anaerobically fermenting products therefrom in comparative example 2;
Fig. 5 is embodiment 3 and Eucheuma muricatum (Gmel.) Web． Van Bos． enzymolysis product alcohol concn curve over time in anaerobically fermenting products therefrom in comparative example 3;
Embodiment
The present invention is illustrated further below in conjunction with accompanying drawing and embodiment.It should be understood that these embodiments are only for illustration of the present invention, and be not used in and limit the scope of the invention.
Embodiment 1:
In the present embodiment, galactosyl algae bio matter is gelidium (Gelidiales).The liquid acids hydrolysate of this galactosyl algae bio matter of anaerobically fermenting obtains ethanol.
Wherein, anaerobically fermenting thalline is Angel Yeast, and cultivating body be mass volume ratio is 2%(w/v) semi-lactosi nutrient solution.Be below concrete anaerobic fermentation process:
(1) parmelia saxatilis dried vegetable frond is pulverized for algae powder; Configuration volumetric concentration is the aqueous sulfuric acid 1 liter of 2%, adds 200 grams of algae powder and fully stirs, and mixes thoroughly to be placed on 125 DEG C of hot environments and to degrade 2 hours, then supplies the biodiversity evaporated; By adding calcium hydroxide after the product suction filtration of gained, pH is adjusted to 4.5, suction filtration removes consequent calcium precipitation, then extracts, and obtains degradation solution after removing organic phase.
After tested, in this degradation solution, each composition parameter is as shown in table 1 below.
Table 1: sugared source content in degradation solution
(2) configuration quality volume ratio is the semi-lactosi nutrient solution 100 milliliters of 2% grams per milliliter, 110 DEG C of high-temperature sterilizations 20 minutes; 1g Angel Yeast powder is added, 38 DEG C of water-baths 30 minutes, then 30 DEG C, 150 rpms induced activation 16 hours in this semi-lactosi nutrient solution; Get wherein 25 milliliters of bacterium liquid, 2500 rpms centrifugal 5 minutes, abandons supernatant, obtain the thalline after induced activation.
(3), in the degradation solution that the thalline obtained after step (2) being processed obtains after adding step (1) process, ferment in the shaking table of 30 DEG C, 150 rpms after sealing.
Comparative example 1:
The present embodiment is the comparative example of above-described embodiment 1.
In the present embodiment, galactosyl algae bio matter is identical with embodiment 1, is gelidium (Gelidiales), is also to obtain ethanol by the liquid acids hydrolysate of this galactosyl algae bio matter of anaerobically fermenting.
The concrete anaerobic fermentation process that the present embodiment adopts, except the cultivation body configured in step (2) is except sucrose nutrient solution, all the other preparation processes are identical with preparation condition and embodiment 1.
Step (2) in the present embodiment is specially: configuration quality volume ratio is the sucrose nutrient solution 100 milliliters of 2% grams per milliliter, 110 DEG C of high-temperature sterilizations 20 minutes; 1g Angel Yeast powder is added, 38 DEG C of water-baths 30 minutes, then 30 DEG C, 150 rpms induced activation 16 hours in this sucrose nutrient solution; Get wherein 25 milliliters of bacterium liquid, 2500 rpms centrifugal 5 minutes, abandons supernatant, obtain the thalline after induced activation.
Above-described embodiment 1 is with comparative example 1, and in the anaerobic fermentation process carried out in step (3), in degradation solution, galactose content change as shown in Figure 1.Wherein, " ◆ " curve is the galactose content curve of embodiment 1, and "●" is the galactose content curve of comparative example 1.As can be seen from Figure 1, add in degradation solution when the yeast of semi-lactosi activation carries out anaerobically fermenting, the most of semi-lactosi after 12 hours that ferments just is used effectively, and semi-lactosi transformation efficiency reaches more than 80%, ferment after 96 hours, 1 gram of gelidium powder can obtain the ethanol of 40.77 milligrams.And the yeast adding sucrose activation in degradation solution is when carrying out anaerobically fermenting, ferment 12 hours, galactose content is substantially unchanged.
Above-described embodiment 1 and the alcohol concn curve contrasted in enforcement 1 in anaerobically fermenting after product are as shown in Figure 2.Wherein, " ◆ " curve is the alcohol concn curve of embodiment 1, and "●" is the alcohol concn curve of comparative example 1.As can be seen from Figure 2, in degradation solution, add the yeast after semi-lactosi induced activation within 12 hours, just produce a large amount of ethanol in fermentation, and in fermentation, the yeast added after sucrose activation does not detect after 72 hours that ethanol produces yet.
Above-described embodiment 1 is with contrast enforcement 1, and in the anaerobic fermentation process carried out in step (3), each parameter of degradation solution producing and ethanol after 24 hours of fermenting is as shown in table 2.Namely, the degradation solution of the yeast of access semi-lactosi induced activation ferments 24 hours, and the transformation efficiency of semi-lactosi reaches 83%, and now ethanol is 79.5% relative to the yield of semi-lactosi, namely semi-lactosi is except other metabolism that small portion is used for volunteer growth needs, and 95% for anaerobically fermenting producing and ethanol.And under equal conditions, in degradation solution, accessing the yeast fermentation 24 hours through sucrose activation, the transformation efficiency of sugar is all lower than 10%.
Table 2: each index parameter of the producing and ethanol after 24 hours of fermenting
Embodiment 2: the later half solid anaerobic digestion of Grateloupia filicina (Wulf.) (Grateloupia) acidolysis
In the present embodiment, galactosyl algae bio matter is Grateloupia filicina (Wulf.) (Grateloupia).The semi-solid state acid hydrolysate of this galactosyl algae bio matter of anaerobically fermenting obtains ethanol.
Wherein, anaerobically fermenting thalline is Angel Yeast, cultivates body to be mass volume ratio be the semi-lactosi solid medium of 4% grams per milliliter.Be below concrete anaerobic fermentation process:
(1) dry for Grateloupia filicina (Wulf.) frond is pulverized for algae powder; Configuration volumetric concentration is the aqueous hydrochloric acid 1 liter of 4%, takes 300 grams of algae powder, adds 1 liter of phosphate aqueous solution and fully stir, and to mix in the baking oven being placed on 100 DEG C high temperature degradation thoroughly 3 hours, then supplies the biodiversity evaporated; The products with sodium hydroxide powder of the solid-liquid mixed style of gained is regulated pH to 5.0, obtains degraded product;
After tested, in this degradation solution, each composition parameter is as shown in table 3 below.
Table 3: sugared source content in degradation solution
(2) configuration quality volume ratio is the semi-lactosi solid medium 25 milliliters of 4% grams per milliliter, 110 DEG C of high-temperature sterilizations 30 minutes; 20 milligrams ~ 50 milligrams Angel Yeasts vibrate mixing in 100 ul sterile water, coat in semi-lactosi solid medium, are placed in 37 DEG C of constant temperature biochemical cultivation case induced activation 24 hours;
(3), in the degradation solution obtained after the thalline on step (2) process rear plate all being accessed step (1) process, ferment in the shaking table of 32 DEG C, 100 rpms after rubber stopper seal.
Comparative example 2:
The present embodiment is the comparative example of above-described embodiment 2.
In the present embodiment, galactosyl algae bio matter is identical with embodiment 2, is Grateloupia filicina (Wulf.), is also to obtain ethanol by the semi-solid state acid hydrolysate of this galactosyl algae bio matter of anaerobically fermenting.
The concrete anaerobic fermentation process that the present embodiment adopts, except the cultivation body configured in step (2) is except glucose culture solution, all the other preparation processes are identical with preparation condition and embodiment 2.
Step (2) in the present embodiment is specially: configuration quality volume ratio is the dextrose solid medium 25 milliliters of 4% grams per milliliter, and 110 DEG C of high-temperature sterilizations 30 minutes, are down flat plate; 20 milligrams ~ 50 milligrams Angel Yeasts vibrate mixing in 100 ul sterile water, coat in semi-lactosi solid medium, are placed in 37 DEG C of constant temperature biochemical cultivation case induced activation 24 hours;
Above-described embodiment 2 is with comparative example 2, and in the anaerobic fermentation process carried out in step (3), in degradation solution, the content of semi-lactosi as shown in Figure 3.Wherein, " slash post " represents the galactose concentration change of embodiment 2; " open tubular column " represents the galactose concentration change of comparative example 2.As can be seen from Figure 3, under same semi-lactosi initial concentration, when the yeast accessed after semi-lactosi activation in Miao's liquid that ferments carries out anaerobically fermenting, galactose content continues to reduce, and ferments after 96 hours, and galactose content is surplus 1.56 grams per liters only.Under equivalent environment, access when the yeast of glucose activation carries out anaerobically fermenting, galactose content slightly falls at fermentation initial (6 hours) content, is down to 37.0 grams per liters from initial 39.42 grams per liters, in fermenting process afterwards, galactose content keeps constant substantially.
Above-described embodiment 2 and the alcohol concn curve contrasted in enforcement 2 in anaerobically fermenting after product are as shown in Figure 4.Wherein, " ◆ " curve is the alcohol concn curve of embodiment 2, and "●" is the alcohol concn curve of comparative example 2.As can be seen from Figure 4, in degradation solution, add the yeast after semi-lactosi induced activation within 3 hours, just start to produce ethanol in fermentation, and in fermentation, the yeast added after glucose activation does not detect after 72 hours that ethanol produces yet.
Above-described embodiment 2 is with contrast enforcement 2, and in the anaerobic fermentation process carried out in step (3), each parameter of the 96 hours secondary fermentation Miao liquid producing and ethanols that ferment is as shown in table 4.That is, the fermentation Miao liquid accessing the yeast of semi-lactosi induced activation ferments 96 hours, and semi-lactosi transformation efficiency reaches 96%, and unit biomass producing and ethanol amount is 79.10 kgs/tonne.And under equal conditions, in fermentation Miao liquid, access the little producing and ethanol not yet constantly of yeast fermentation 96 through glucose activation.
Table 4: each index parameter of the producing and ethanol after 96 hours of fermenting
Embodiment 3: Eucheuma muricatum (Gmel.) Web． Van Bos． (Eucheuma) enzymolysis later half solid product fermentation producing and ethanol
In the present embodiment, galactosyl algae bio matter is Eucheuma muricatum (Gmel.) Web． Van Bos． (Eucheuma).The enzymolysis semi-solid state product of this galactosyl algae bio matter of anaerobically fermenting obtains ethanol.
Wherein, anaerobically fermenting thalline is transfering loop yeast saccharomyces cerevisiae, and cultivating body be mass volume ratio is 3%(w/v) semi-lactosi nutrient solution.Be below concrete anaerobic fermentation process:
(1) kylin dried vegetable frond is pulverized for algae powder; Configuration volumetric concentration is the aqueous nitric acid 1 liter of 4%, takes 200 grams of Eucheuma muricatum (Gmel.) Web． Van Bos． powder, adds 1 liter of aqueous nitric acid and fully stir, by mix thoroughly be placed on 150 DEG C environment in high temperature degradation 1.5 hours, then supply the biodiversity evaporated; The products with sodium hydroxide of gained is regulated pH to 6.5, then adds the α-amylase of 50 ~ 200U/ gram, degrade 72 hours for 60 DEG C, then adjust pH to 5.0 through hydrochloric acid, then the saccharifying enzyme adding 50 ~ 200U/ gram continues enzymolysis 72 hours under the environment of 60 DEG C; Getting degraded product 50 milliliters adds in anaerobism bottle, 80 DEG C of high-temperature sterilizations 1 hour.
After tested, in this degradation solution, each moiety parameter is as shown in table 5 below.
Table 5: sugared source content in degradation solution
(2) configuration quality volume ratio is the semi-lactosi nutrient solution 100 milliliters of 3% grams per milliliter, 110 DEG C of high-temperature sterilizations 30 minutes; A transfering loop yeast saccharomyces cerevisiae is accessed, 30 DEG C, 200 revs/min induced activation 10 hours from inclined-plane; Get wherein 40 milliliters of bacterium liquid, 2500 rpms centrifugal 5 minutes, abandons supernatant, obtain the thalline after induced activation.
(3), in the degradation solution that the thalline obtained after step (2) being processed obtains after adding step (1) process, ferment in the shaking table of 30 DEG C, 150 revs/min after rubber stopper seal mouth.
Comparative example 3:
The present embodiment is the comparative example of above-described embodiment 3.
In the present embodiment, galactosyl algae bio matter is identical with embodiment 3, is Eucheuma muricatum (Gmel.) Web． Van Bos．, is also to obtain ethanol by the enzymolysis semi-solid state product of this galactosyl algae bio matter of anaerobically fermenting.
The concrete anaerobic fermentation process that the present embodiment adopts, except the cultivation body configured in step (2) is except sucrose nutrient solution, all the other preparation processes are identical with preparation condition and embodiment 3.
Step (2) in the present embodiment is specially: configuration quality volume ratio is the sucrose nutrient solution 100 milliliters of 3% grams per milliliter, 110 DEG C of high-temperature sterilizations 30 minutes; A transfering loop yeast saccharomyces cerevisiae is accessed, 30 DEG C, 200 revs/min induced activation 10 hours from inclined-plane; Get wherein 40 milliliters of bacterium liquid, 2500 rpms centrifugal 5 minutes, abandons supernatant, obtains the thalline after activating.
Above-described embodiment 3 and the alcohol concn curve contrasted in enforcement 3 in anaerobically fermenting after product are as shown in Figure 5.Wherein, " ◆ " curve is the alcohol concn curve of embodiment 3, and "●" is the alcohol concn curve of comparative example 3.As can be seen from Figure 5, in degradation solution, add the yeast after semi-lactosi induced activation within 3 hours, just start to produce ethanol in fermentation, within 36 hours, complete fermentation in fermentation.And add the yeast after sucrose activation just detects 0.5 grams per liter for 36 hours ethanol generation in fermentation, and in Miao's liquid that ferments, alcohol concn rate of growth is slow.
Above-described embodiment 3 is with contrast enforcement 3, and in the anaerobic fermentation process carried out in step (3), the parameters of degradation solution producing and ethanol after 48 hours that ferments is as shown in table 6.As seen from the table, after the degradation solution of the yeast of access semi-lactosi induced activation ferments 48 hours, the semi-lactosi in degradation solution exhausts, and is now 94% relative to the alcohol getting rate of semi-lactosi, namely most of semi-lactosi is for the producing and ethanol that ferments, and the amount of unit biomass producing and ethanol is 45.0 kgs/tonne.And under equal conditions, access the yeast of sucrose activation in fermentation Miao liquid after, fermenting starts ferment galactose producing and ethanol for 36 hours, namely relative to the former, start time is slow, and producing and ethanol efficiency is low.
Table 6: each index parameter of the producing and ethanol after 48 hours of fermenting
Embodiment 4: liquid product fermentation producing and ethanol after fragrant plant mentioned in ancient texts (Gracilaria) acidolysis
In the present embodiment, galactosyl algae bio matter is fragrant plant mentioned in ancient texts (Gracilaria).The acidolysis liquid product of this galactosyl algae bio matter of anaerobically fermenting obtains ethanol.
Wherein, anaerobically fermenting thalline is Angel Yeast, and cultivating body be mass volume ratio is 5%(w/v) semi-lactosi nutrient solution.Be below concrete anaerobic fermentation process:
(1) dry for fragrant plant mentioned in ancient texts frond is pulverized for algae powder; Configuration volumetric concentration is the aqueous sulfuric acid 1 liter of 1.5%, takes 300 grams of fragrant plant mentioned in ancient texts powder, adds 1 liter of aqueous sulfuric acid and fully stir, by mix thoroughly be placed on 115 DEG C environment in high temperature degradation 1.5 hours, then supply the biodiversity evaporated; Degradation solution is obtained after being filtered by the product of gained.Regulate this degradation solution pH to 6.0 with calcium hydroxide, filter and remove solid part, then take out 50 milliliters, 90 DEG C of high-temperature sterilizations 1 hour.
After tested, each moiety parameter in the degradation solution obtained is processed through step (1) as shown in table 7 below.
Table 7: sugared source content in degradation solution
(2) configuration quality volume ratio is the semi-lactosi nutrient solution 100 milliliters of 5% grams per milliliter, 110 DEG C of high-temperature sterilizations 30 minutes; Take 1.0 grams of Angel Yeast powder, add in 100 milliliters of semi-lactosi nutrient solutions, 35 DEG C, 200 revs/min induced activation 10 hours; Get wherein 50 milliliters of bacterium liquid, 2500 rpms centrifugal 5 minutes, abandons supernatant, obtain the thalline after induced activation.
(3), in the degradation solution that the thalline obtained after step (2) being processed obtains after adding step (1) process, ferment in the shaking table of 30 DEG C, 150 revs/min after rubber stopper seal mouth.
Comparative example 4:
The present embodiment is the comparative example of above-described embodiment 4.
In the present embodiment, galactosyl algae bio matter is identical with embodiment 4, is fragrant plant mentioned in ancient texts, is also to obtain ethanol by the acidolysis liquid product of this galactosyl algae bio matter of anaerobically fermenting.
The concrete anaerobic fermentation process that the present embodiment adopts, except the cultivation body configured in step (2) is except sucrose nutrient solution, all the other preparation processes are identical with preparation condition and embodiment 4.
Step (2) in the present embodiment is specially: configuration quality volume ratio is the sucrose nutrient solution 100 milliliters of 5% grams per milliliter, 110 DEG C of high-temperature sterilizations 30 minutes; Take 1.0 grams of Angel Yeast powder, add in 100 milliliters of semi-lactosi nutrient solutions, 35 DEG C, 200 revs/min induced activation 10 hours; Get wherein 50 milliliters of bacterium liquid, 2500 rpms centrifugal 5 minutes, abandons supernatant, obtain the thalline after induced activation.
Above-described embodiment 4 is with contrast enforcement 4, and in the anaerobic fermentation process carried out in step (3), the parameters of degradation solution producing and ethanol after 36 hours that ferments is as shown in table 8.The degradation solution of the yeast of access semi-lactosi induced activation is in fermentation after 36 hours, and in fermented liquid, the content of ethanol reaches 16.7g/L.Now, the semi-lactosi in fermented liquid exhausts, and ethanol is 88.9% relative to the alcohol getting rate of semi-lactosi, and illustrate that most of semi-lactosi is for the producing and ethanol that ferments, the amount of unit biomass producing and ethanol reaches 55.7 kgs/tonne.And under equal conditions, access the yeast of sucrose activation in degradation solution after, ferment after 36 hours, the ethanol content recorded is only 3.91 grams often liter, and alcohol getting rate is 10.63%, and its fermentation efficiency and output are all well below embodiment 4.
Table 8: each index parameter of the producing and ethanol after 36 hours of fermenting
Above-described embodiment has been described in detail technical scheme of the present invention; be understood that and the foregoing is only specific embodiments of the invention; be not limited to the present invention; all make in spirit of the present invention any amendment, supplement or similar fashion substitute etc., all should be included within protection scope of the present invention.
Claims (10)
1. a fermentation process for galactosyl algae bio matter, the method comprises following process: described algae bio matter degraded, obtain the process of degraded product; Adopt and cultivate body induced activation thalline; Thalline after induced activation is accessed the process that this degraded product carries out anaerobically fermenting; It is characterized in that: described thalline is the yeast containing gal operon or the intestinal bacteria containing gal operon; In described thalline induced activation process, cultivating body is semi-lactosi nutrient solution or solid semi-lactosi substratum.
2. the fermentation process of galactosyl algae bio matter as claimed in claim 1, is characterized in that: described galactosyl algae bio matter comprises red algae biomass.
3. the fermentation process of galactosyl algae bio matter as claimed in claim 1, is characterized in that: described red algae biomass comprise Gelidium (Gelidium), centipede Trentepohlia (Grateloupia), Eucheuma (Eucheuma) and Gracilaria (Gracilaria) kelp.
4. the fermentation process of galactosyl algae bio matter as claimed in claim 1, is characterized in that: the form of described degraded product comprises solid, semisolid and liquid.
5. the fermentation process of galactosyl algae bio matter as claimed in claim 1, it is characterized in that: in described thalline induced activation process, thalline is 0.1% (w/v) ~ 2% (w/v), more preferably 0.5% (w/v) with the mass volume ratio of cultivation body.
6. the fermentation process of galactosyl algae bio matter as claimed in claim 1, is characterized in that: described semi-lactosi is cultivated in body, and semi-lactosi mass percent concentration is 0.5% ~ 50%.
7. the fermentation process of galactosyl algae bio matter as claimed in claim 1, it is characterized in that: described thalline induced activation process is: thalline is added in semi-lactosi nutrient solution or solid semi-lactosi substratum, induced activation 2 hours ~ 24 hours at the temperature of 25 DEG C ~ 40 DEG C, hunting speed is 0 ~ 300 rpm.
8. the fermentation process of galactosyl algae bio matter as claimed in claim 1, is characterized in that: the thalline after described induced activation and the volume ratio of degraded product are 1:1 ~ 1:10.
9. the fermentation process of galactosyl algae bio matter as claimed in claim 1, is characterized in that: described anaerobically fermenting carries out under 25 DEG C ~ 35 DEG C conditions; Described anaerobic fermentation process carries out 12 hours ~ 168 hours.
10. the fermentation process of the galactosyl algae bio matter as described in claim arbitrary in claim 1 to 9, is characterized in that: described anaerobically fermenting product is biofuel or chemical intermediate, comprises alcohols organic substance and alkanes organic substance.
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| CN101215590A (en) * | 2007-12-27 | 2008-07-09 | 山东大学 | Method for removing monosaccharide component from oligomerization galactose by utilizing immobilized yeast |
| CN102559799A (en) * | 2011-01-27 | 2012-07-11 | 河北工业大学 | Preparation method for algae endophytic fungi exocellular polysaccharide |
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| CN101215590A (en) * | 2007-12-27 | 2008-07-09 | 山东大学 | Method for removing monosaccharide component from oligomerization galactose by utilizing immobilized yeast |
| CN102559799A (en) * | 2011-01-27 | 2012-07-11 | 河北工业大学 | Preparation method for algae endophytic fungi exocellular polysaccharide |
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| CN108165587A (en) * | 2018-03-23 | 2018-06-15 | 福州大学 | A kind of method that biological butanol is prepared using algal polysaccharides agar |
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