CN104222695A - Feed for medical DPF donor pigs and preparation method thereof - Google Patents
Feed for medical DPF donor pigs and preparation method thereof Download PDFInfo
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- CN104222695A CN104222695A CN201410534531.9A CN201410534531A CN104222695A CN 104222695 A CN104222695 A CN 104222695A CN 201410534531 A CN201410534531 A CN 201410534531A CN 104222695 A CN104222695 A CN 104222695A
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Abstract
The invention aims at providing a feed for medical DPF donor pigs and a preparation method thereof. By means of the feed for the medical DPF donor pigs and the preparation method, the nutritional requirement and sterilization requirement can be met simultaneously, morbidity of the pigs can be reduced, cost can be reduced, economic benefit can be improved, growth quality and immunity of the medical DPF donor pigs can be improved, a preservation period and expiration date of the feed can be prolonged, and the feed can be completely absorbed by the medical DPF donor pigs.
Description
Technical field
The present invention relates to medical donor pig's feed of a kind of DPF and preparation method thereof.
Background technology
Heterograft nothing specifies pathogen (Designed Pathogen free, DPF) donor pig refers to through artificial feeding and reaches the miniature pig of World Health Organization's donor animal standard, require that nothing appointment pathogen in body, genetic background are clear and definite or it is clear to originate, 12 monthly age body weight are no more than 60kg, can be used for medical donor, scientific research, teaching, production and calibrating and other scientific experiments.DPF swine rearing requires to require higher than no-special pathogen level animal used as test, need to raise in superior cleanliness barrier environment, isolate with external environment, microorganism in strict control feeding environment, and require that pig grows up healthy and sound in raising, lipids contents can not be too high, also will meet strict infectious disease prevention and control standard.DPF pig is due to the requirement of its medical donor in addition, so can not use antibiotic and hormonal substance in feed, needs to add the immunity that some special compositions safely and effectively improve pig, suppresses growth of microorganism, ensure the clean aseptic of pig.Clear stipulaties in " People's Republic of China's veterinary biologics quality standard " and " People's Republic of China's regulations " that China is existing: three grades of (no-special pathogen level) animals used as test must use sterilizing feed.For DPF pig, the sterilization treatment of feed just ensures the necessary means of its quality especially.Current production adopts maximum feed sterilization technology be high temperature and high pressure steam sterilizing, its technology maturation is easy to grasp.But it is larger to adopt autoclaving nutritional labeling to destroy.And the feed shelf-life prepared is short, the feedstuff-meat ratio of the DPF pig of long-term edible this feed is high, deficiency in economic performance, and the immunity of DPF pig is poor.
Summary of the invention
The object of the present invention is to provide a kind ofly can meet nutritional need and sterilizing requirement simultaneously, reduce the pig incidence of disease, reduce costs, the growth quality of DPF medical donor pig of increasing economic efficiency, improve and immunity, the freshness date of prolongation feed, the shelf-life, the medical donor pig's feed of DPF that can be absorbed completely by DPF medical donor pig.
Another object of the present invention is to the preparation method that a kind of simple above-mentioned feed is provided.
Concrete technical scheme:
The medical donor pig's feed of a kind of DPF, by corn flour 60 ~ 65 mass parts, dregs of beans 18 ~ 22 mass parts, wheat bran 5.0 ~ 7.0 mass parts, fishbone dust 5.0 ~ 7.0 mass parts, calcium monohydrogen phosphate 1.0 ~ 2.0 mass parts, stone flour 0.5 ~ 1.5 mass parts, soya-bean oil 0.5 ~ 1.0 mass parts, salt 0.5 ~ 1.0 mass parts, CLA 0.3 ~ 1.0 quality is divided, green tea powder 0.5 ~ 1.5 mass parts, saccharicter-penin 0.5 ~ 1.5 mass parts, allicin and dried orange peel amount to 1.0 ~ 1.5 mass parts, premix 2 ~ 3 mass parts mixes, temperature 121 ~ 126 DEG C, under the high steam of pressure 102.97 ~ 137.30kPa, sterilizing 20 ~ 30min, dry at 70-85 DEG C, make moisture lower than 5%, obtain feed for subsequent use,
By VA, VD, VE, VK, VB
1, VB
2, VB
6, VB
12, niacin, pantothenic acid, lysine, methionine and cystine mixing, after filtration sterilization, obtain additive; Containing VA300 ~ 400 μ g, VD 14 ~ 15 μ g, VE 1 ~ 1.5mg, VK 8 ~ 9g, VB in every 1 part of additive
14.5 ~ 5g, VB
24 ~ 5g, VB
63 ~ 4g, VB
1219 ~ 20g, niacin 9 ~ 11g, pantothenic acid 7 ~ 9g, lysine 4 ~ 6kg, methionine 0.5 ~ 1kg, cystine 0.8 ~ 1.2kg;
Additive is added in feed for subsequent use through spraying coating process, in every 1 ton of feed for subsequent use, adds additive 0.5-2 part, dry at 70-85 DEG C, obtain the medical donor pig's feed of DPF.
The preparation method of the medical donor pig's feed of DPF, by corn flour 60 ~ 65 mass parts, dregs of beans 18 ~ 22 mass parts, wheat bran 5.0 ~ 7.0 mass parts, fishbone dust 5.0 ~ 7.0 mass parts, calcium monohydrogen phosphate 1.0 ~ 2.0 mass parts, stone flour 0.5 ~ 1.5 mass parts, soya-bean oil 0.5 ~ 1.0 mass parts, salt 0.5 ~ 1.0 mass parts, CLA 0.3 ~ 1.0 quality is divided, green tea powder 0.5 ~ 1.5 mass parts, saccharicter-penin 0.5 ~ 1.5 mass parts, allicin and dried orange peel amount to 1.0 ~ 1.5 mass parts, premix 2 ~ 3 mass parts mixes, temperature 121 ~ 126 DEG C, under the high steam of pressure 102.97 ~ 137.30kPa, sterilizing 20 ~ 30min, then dry at 70-85 DEG C, make moisture lower than 5%, obtain feed for subsequent use, by VA, VD, VE, VK, VB
1, VB
2, VB
6, VB
12, niacin, pantothenic acid, lysine, methionine and cystine mixing, add water and be prepared into liquid preparation, after filtration sterilization, obtain additive, containing VA 300 ~ 400 μ g, VD 14 ~ 15 μ g, VE 1 ~ 1.5mg, VK 8 ~ 9g, VB in every 1 part of additive
14.5 ~ 5g, VB
24 ~ 5g, VB
63 ~ 4g, VB
1219 ~ 20g, niacin 9 ~ 11g, pantothenic acid 7 ~ 9g, lysine 4 ~ 6kg, methionine 0.5 ~ 1kg, cystine 0.8 ~ 1.2kg, additive is added in feed for subsequent use, in every 1 ton of feed for subsequent use, adds additive 0.5-2 part, dry at 70-85 DEG C, obtain the medical donor pig's feed of DPF, containing trace element and the edible premix carrier of pig in wherein said premix.
Wherein VA represents vitamin A, and all the other " V " all represent vitamin.
Preferred above-mentioned green tea powder 0.8 ~ 1.2 mass parts, saccharicter-penin 0.5 ~ 0.8 mass parts, allicin and dried orange peel amount to 0.8 ~ 1.5 mass parts.
The mass ratio of above-mentioned allicin and dried orange peel is 1:2-1:6.
Preferably add additive 1 part in every 1 ton of feed for subsequent use.
The mass ratio of preferred allicin and dried orange peel is 1:5.
Wherein preferably corn flour 60 ~ 62 mass parts, dregs of beans 19 ~ 21 mass parts, wheat bran 6 ~ 6.5 mass parts, fishbone dust 6 ~ 6.5 mass parts, calcium monohydrogen phosphate 1.0 ~ 1.5 mass parts, stone flour 0.8 ~ 1.2 mass parts, soya-bean oil 0.8 ~ 1.0 mass parts, salt 0.5 ~ 0.8 mass parts, CLA 0.5 ~ 0.8 quality are divided, green tea powder 0.8 ~ 1.2 mass parts, saccharicter-penin 0.5 ~ 0.8 mass parts, allicin and dried orange peel amount to 0.8 ~ 1.5 mass parts, premix 2.5 ~ 3 mass parts.
Every kilogram of described premix contains: carrier 350 ~ 450g, i.e. fishbone dust 350 ~ 450g; Trace element 550 ~ 650g, i.e. B B-complex 200 ~ 240g, folic acid 15 ~ 25g, zinc sulfate 25 ~ 35g, sodium selenite 10 ~ 15g, KI 15 ~ 20g, lysine 60 ~ 70g, methionine 15 ~ 20g, threonine 15 ~ 20g, tryptophan 15 ~ 20g, ferrous sulfate 20 ~ 30g, Pfansteihl 95 ~ 105g, citric acid 45 ~ 55g.
B B-complex is containing more than one in vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamine D3, vitamin E or Vitamin K3.
Additive is by the method sterilizing of filtration sterilization;
The ceramic membrane that filtration sterilization equipment can use aperture to be 150-220nm.
The particle diameter of feed for subsequent use is 45 ~ 55 orders.
Beneficial effect of the present invention
Feed formula of the present invention is scientific and reasonable, can not only meet nutritional need and the sterilizing requirement of the medical donor pig of DPF, and result shows, the feed conversion rate of the DPF medical donor pig of edible feed of the present invention is high, can maintain DPF medical donor pig healthy growth.Feed Energy of the present invention meets the growth of DPF donor pig and the nutritional labeling needed for growth, and use the upgrowth situation of the medical donor pig of the DPF of feed of the present invention better, feed conversion rate is high.Especially by adding the special composition such as green tea powder, saccharicter-penin, allicin and dried orange peel compound, not only improve the immunity of DPF pig and the ability of anti-bacteria and anti-virus, ensure that the security of DPF pig as medical donor pig, and making the utilization rate of feed improve 93%, economic benefit improves 25%.The present invention fills a prescription reasonable and practical, controls cost to greatest extent, and efficiency is high, improves growth quality and the immunity of medical DPF donor pig, can be absorbed completely by the medical donor pig of DPF.In addition, by detecting the special pathogen in feed, result is feminine gender, meets the medical requirement of DPF medical donor pig completely.The present invention is by the optimization to feed composition and content, add the coordinated optimized with sterilising conditions, can obtain a kind ofly meeting nutritional need and sterilizing requirement simultaneously, reduce the pig incidence of disease, improve the growth quality of DPF medical donor pig and immunity, the freshness date of prolongation feed, shelf-life, the medical donor pig's feed of DPF that can be absorbed completely by DPF medical donor pig.
Detailed description of the invention
In order to understand the present invention better, describe technical scheme of the present invention in detail with instantiation below, but the present invention is not limited thereto.
The pig fed in following experimentation is the medical donor pig of DPF.
Embodiment 1
By 600kg corn flour, 200kg dregs of beans, 70kg wheat bran, 50kg fishbone dust, 15kg calcium monohydrogen phosphate, 6.5kg stone flour, 5kg soya-bean oil, 5kg salt, CLA 7.5Kg, 10kg green tea powder, 5kg saccharicter-penin, 15kg allicin and dried orange peel, every 20 kilograms of premixes contain: fishbone dust 8000g, B B-complex 4400g, folic acid 400g, zinc sulfate 600g, sodium selenite 200g, KI 400g, lysine 1300g, methionine 400g, threonine 400g, tryptophan 400g, ferrous sulfate 500g, Pfansteihl 2000g, citric acid 1000g, at 121 DEG C after mixing, 102.97kPa under the high steam of pressure, sterilizing 30min sterilizing, place 48h at 70 ~ 85 DEG C to dry, make moisture lower than 5%, wherein, the mass ratio of allicin and dried orange peel is 1:5.
Every 1 part of additive contains: VA 350 μ g, VD 15 μ g, VE 1.2mg, VK 8.5g, VB
15g, VB
24.5g, VB
63.5, VB
1220g, niacin 10g, pantothenic acid 8g, lysine 5kg, methionine 0.7kg, cystine 1kg are prepared into liquid preparation, filtration sterilization.
Add 1 part of additive according in every 1 ton of feed for subsequent use, additive is added in particle pig feed through spraying coating process, in 70-85 DEG C, place 24h dry.Feed packs through the nylon-polyethylene compound package material of ultraviolet radiation sterilization subsequently, every bag of 80kg, the feed prepared be stored in 20-30 DEG C, relative humidity lower than 50% indoor.Finish using in one month.
Comparative example 1
Except not adding additive, the feed prepared according to the method that embodiment 1 is same.
In each following effect test example, be equivalent, etc. meal feed the feed of embodiment and comparative example.
Effect test example 1
The pig 12 of 150 ~ 175 ages in days raised respectively by the feed using embodiment 1 and comparative example 1 to prepare, and is divided into 3 groups at random, often organizes 4, detects after feeding 2 months to the growing state of DPF pig and blood fat etc.
Table 1 DIFFERENT FEED is on the impact of the growing state of pig
As can be seen from Table 1, the daily gain of feeding the pig of the feed of embodiment 1 is larger, and feedstuff-meat ratio is lower, illustrates that the conversion ratio of this feed is higher.
Table 2
(content is mmol/L)
As shown in Table 2, in serum, use the T-CHOL (TC) in 2 months serum of the pig of feed described in comparative example 1, low-density lipoprotein (LDL) content all higher than the pig of the forage feed of use embodiment 1, illustrate that feed described in embodiment 1 is in the better effects if maintaining total cholesterol level and LDL-C stable content in DPF grower pigs blood.
Comparative example 2
Except not adding green tea powder, saccharicter-penin, allicin and dried orange peel, according to the method that embodiment 1 is same, prepare feed.
Comparative example 3
Except green tea powder be 3kg, saccharicter-penin be 2kg, allicin and dried orange peel is except 5kg, according to the method that embodiment 1 is same, prepares feed.
Effect test example 2
The forage feed DPF pig using embodiment 1 and comparative example 2,3 to prepare, chooses 150 age in days DPF pigs and is divided into two groups at random, and often organize 4 DPF pigs, feed 1 month, get the biochemical indicator of Virus monitory DPF pig, result is as shown in table 3.
Table 3
As can be drawn from Table 3, the blood-sugar content of the DPF pig using the obtained feed formula of embodiment 1 to feed is higher, illustrates that its growing state is better.And the content of its alkaline phosphatase is also higher, illustrate that its metabolism degree is higher, the speed of growth is faster.And in its serum the concentration of gamma interferon and CD4+/CD8+ higher, illustrate that the metabolism of the DPF pig that embodiment 1 feed formula is fed is better, immunity is better.
Effect test example 3
After three weeks, to the DPF pig of forage feed using the embodiment of the present invention 1 to prepare, parallelly carry out 8 groups, often organize three DPF pigs, get the detection that serum sample and excreta carry out special pathogen, testing result is as shown in table 4.
Table 4.DPF pig special pathogen testing result
As can be seen from Table 4, the sterilization effect of the DPF pig using the obtained feed formula of embodiment 1 to feed is good.The freshness date of feed, long shelf-life.
Claims (10)
1. the medical donor pig's feed of DPF, it is characterized in that, by corn flour 60 ~ 65 mass parts, dregs of beans 18 ~ 22 mass parts, wheat bran 5.0 ~ 7.0 mass parts, fishbone dust 5.0 ~ 7.0 mass parts, calcium monohydrogen phosphate 1.0 ~ 2.0 mass parts, stone flour 0.5 ~ 1.5 mass parts, soya-bean oil 0.5 ~ 1.0 mass parts, salt 0.5 ~ 1.0 mass parts, CLA 0.3 ~ 1.0 quality is divided, green tea powder 0.5 ~ 1.5 mass parts, saccharicter-penin 0.5 ~ 1.5 mass parts, allicin and dried orange peel amount to 1.0 ~ 1.5 mass parts, premix 2 ~ 3 mass parts mixes, temperature 121 ~ 126 DEG C, under the high steam of pressure 102.97 ~ 137.30kPa, sterilizing 20 ~ 30min, then dry at 70-85 DEG C, make moisture lower than 5%, obtain feed for subsequent use, by VA, VD, VE, VK, VB
1, VB
2, VB
6, VB
12, niacin, pantothenic acid, lysine, methionine and cystine mixing, add water and be prepared into liquid preparation, after filtration sterilization, obtain additive, containing VA 300 ~ 400 μ g, VD 14 ~ 15 μ g, VE 1 ~ 1.5mg, VK 8 ~ 9g, VB14.5 ~ 5g, VB24 ~ 5g, VB63 ~ 4g, VB in every 1 part of additive
1219 ~ 20g, niacin 9 ~ 11g, pantothenic acid 7 ~ 9g, lysine 4 ~ 6kg, methionine 0.5 ~ 1kg, cystine 0.8 ~ 1.2kg, additive is added in feed for subsequent use, in every 1 ton of feed for subsequent use, adds additive 0.5-2 part, dry at 70-85 DEG C, obtain the medical donor pig's feed of DPF, containing trace element and the edible premix carrier of pig in wherein said premix.
2. feed according to claim 1, is characterized in that, described green tea powder 0.8 ~ 1.2 mass parts, saccharicter-penin 0.5 ~ 0.8 mass parts, allicin and dried orange peel amount to 0.8 ~ 1.5 mass parts.
3. feed according to claim 1, is characterized in that, the mass ratio of allicin and dried orange peel is 1:2-1:6.
4. feed according to claim 1, is characterized in that, adds additive 1 part in every 1 ton of feed for subsequent use.
5. the feed according to claim 1 or 3, is characterized in that, the mass ratio of allicin and dried orange peel is 1:5.
6. feed according to claim 1, it is characterized in that, by corn flour 60 ~ 62 mass parts, dregs of beans 19 ~ 21 mass parts, wheat bran 6 ~ 6.5 mass parts, fishbone dust 6 ~ 6.5 mass parts, calcium monohydrogen phosphate 1.0 ~ 1.5 mass parts, stone flour 0.8 ~ 1.2 mass parts, soya-bean oil 0.8 ~ 1.0 mass parts, salt 0.5 ~ 0.8 mass parts, CLA 0.5 ~ 0.8 quality is divided, green tea powder 0.8 ~ 1.2 mass parts, saccharicter-penin 0.5 ~ 0.8 mass parts, allicin and dried orange peel amount to 0.8 ~ 1.5 mass parts, the mixing of premix 2.5 ~ 3 mass parts.
7. the feed according to claim 1 or 6, is characterized in that, every kilogram of described premix contains: carrier 350 ~ 450g, i.e. fishbone dust 350 ~ 450g; Trace element 550 ~ 650g, i.e. B B-complex 200 ~ 240g, folic acid 15 ~ 25g, zinc sulfate 25 ~ 35g, sodium selenite 10 ~ 15g, KI 15 ~ 20g, lysine 60 ~ 70g, methionine 15 ~ 20g, threonine 15 ~ 20g, tryptophan 15 ~ 20g, ferrous sulfate 20 ~ 30g, Pfansteihl 95 ~ 105g, citric acid 45 ~ 55g.
8. the preparation method described in any one of claim 1-7, it is characterized in that, by corn flour 60 ~ 65 mass parts, dregs of beans 18 ~ 22 mass parts, wheat bran 5.0 ~ 7.0 mass parts, fishbone dust 5.0 ~ 7.0 mass parts, calcium monohydrogen phosphate 1.0 ~ 2.0 mass parts, stone flour 0.5 ~ 1.5 mass parts, soya-bean oil 0.5 ~ 1.0 mass parts, salt 0.5 ~ 1.0 mass parts, CLA 0.3 ~ 1.0 quality is divided, green tea powder 0.5 ~ 1.5 mass parts, saccharicter-penin 0.5 ~ 1.5 mass parts, allicin and dried orange peel amount to 1.0 ~ 1.5 mass parts, premix 2 ~ 3 mass parts mixes, temperature 121 ~ 126 DEG C, under the high steam of pressure 102.97 ~ 137.30kPa, sterilizing 20 ~ 30min, then dry at 70-85 DEG C, make moisture lower than 5%, obtain feed for subsequent use, by VA, VD, VE, VK, VB
1, VB
2, VB
6, VB
12, niacin, pantothenic acid, lysine, methionine and cystine mixing, add water and be prepared into liquid preparation, after filtration sterilization, obtain additive, containing VA 300 ~ 400 μ g, VD 14 ~ 15 μ g, VE 1 ~ 1.5mg, VK 8 ~ 9g, VB14.5 ~ 5g, VB24 ~ 5g, VB63 ~ 4g, VB in every 1 part of additive
1219 ~ 20g, niacin 9 ~ 11g, pantothenic acid 7 ~ 9g, lysine 4 ~ 6kg, methionine 0.5 ~ 1kg, cystine 0.8 ~ 1.2kg, additive is added in feed for subsequent use, in every 1 ton of feed for subsequent use, adds additive 0.5-2 part, dry at 70-85 DEG C, obtain the medical donor pig's feed of DPF, containing trace element and the edible premix carrier of pig in wherein said premix.
9. method according to claim 8, is characterized in that, described green tea powder 0.8 ~ 1.2 mass parts, saccharicter-penin 0.5 ~ 0.8 mass parts, allicin and dried orange peel amount to 0.8 ~ 1.5 mass parts.
10. method according to claim 8, is characterized in that, every kilogram of described premix contains: carrier 350 ~ 450g, i.e. fishbone dust 350 ~ 450g; Trace element 550 ~ 650g, i.e. B B-complex 200 ~ 240g, folic acid 15 ~ 25g, zinc sulfate 25 ~ 35g, sodium selenite 10 ~ 15g, KI 15 ~ 20g, lysine 60 ~ 70g, methionine 15 ~ 20g, threonine 15 ~ 20g, tryptophan 15 ~ 20g, ferrous sulfate 20 ~ 30g, Pfansteihl 95 ~ 105g, citric acid 45 ~ 55g.
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Address after: 410600, No. 128 Jinshui East Road, Jinzhou new district, Changsha County, Hunan, Ningxiang Applicant after: HUNAN XENO LIFE SCIENCE CO., LTD. Address before: 410600, No. 128 Jinshui East Road, Jinzhou new district, Changsha County, Hunan, Ningxiang Applicant before: HUNAN XENO LIFE SCIENCE CO., LTD. |
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