CN104222025B - Defence type trichogramma honeybee card and preparation method thereof - Google Patents

Defence type trichogramma honeybee card and preparation method thereof Download PDF

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Publication number
CN104222025B
CN104222025B CN201410520150.5A CN201410520150A CN104222025B CN 104222025 B CN104222025 B CN 104222025B CN 201410520150 A CN201410520150 A CN 201410520150A CN 104222025 B CN104222025 B CN 104222025B
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China
Prior art keywords
ovum
layer
trichogramma
honeybee
card
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CN201410520150.5A
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CN104222025A (en
Inventor
太红坤
白树雄
顾中量
王根权
郑礼
张峰
刘峙
王振营
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YUNNAN DEHONG DAI AND JINGPO AUTONOMOUS PREFECTURE PLANT PROTECTION AND PLANT QUARANTINE STATION
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YUNNAN DEHONG DAI AND JINGPO AUTONOMOUS PREFECTURE PLANT PROTECTION AND PLANT QUARANTINE STATION
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Priority to CN201410520150.5A priority Critical patent/CN104222025B/en
Publication of CN104222025A publication Critical patent/CN104222025A/en
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Abstract

The present invention relates to a kind of defence type trichogramma honeybee card and preparation method thereof, described defence type trichogramma honeybee card includes ovum layer, porose cover layer, and be attached with Trichogramma ovum on ovum layer;Containing aperture on porose cover layer;Double-layer structure is glued together, forms defence type trichogramma honeybee card.Present invention also offers the preparation method of above-mentioned defence type trichogramma honeybee card.The honeybee card of preparation can preferably solve the predation to ovum of the field natural enemy;Egg parasitoid can be protected do not damaged by rainwater shower and direct sunlight;Described honeybee card can also be divided into some chalcid flies to block equably, can accurate quantitative analysis ground trichogramma releases in the unit are of field;And this kind of honeybee card is with low cost, material therefor is simple and environmentally-friendly;And production operation is simple, rapidly, conveniently.

Description

Defence type trichogramma honeybee card and preparation method thereof
Technical field
The invention belongs to raising or the cultivation apparatus technical field of invertebrates, more particularly relate to a kind of defence Formula trichogramma honeybee card and preparation method thereof.
Background technology
Trichogramma trichogrammatid, Hymenoptera (Hymennptera) Trichogrammatidae (Trichogrammat- Idae) little parasitic wasp, espespecially for Trichogramma spp. (Tricltogramma), adult length less than 1 millimeter, wing in Pyriform, the single vein of tool and spike echinid, attached joint 3 saves, hence it is evident that.Larva is parasitic in the ovum of moth class, and therefore one The natural enemy of the highly advantageous value of class, is used for the Biological control of crops.
Carrying out Biological control then needs substantial amounts of Trichogramma ovum to render to field, and current input mode is many Use the mode that egg parasitoid is glued in certain substrate make open honeybee card.Make Trichogramma honeybee card It is to utilize one of trichogramma Biological control important step on a large scale, therefore uses which kind of mode to prepare honeybee card and preparation The problems such as which type of honeybee blocks for ensureing the survival rate that field is thrown in, effective percentage are the most crucial.And it is the most domestic Trichogramma production plant mainly uses manual hairbrush brush coating, manual double faced adhesive tape viscose glue, mimeograph gluing and air pressure spray The method gluings such as glue, then adhere to egg parasitoid, finally complete honeybee card and make.Hairbrush brush coating and manual double faced adhesive tape take Time take a lot of work, and hairbrush brush coating and mimeograph glue uneven, there is egg parasitoid problem of skewness in honeybee paperboard. Air pressure glue spraying is then relatively costly, the atomization of glue and cause environmental pollution, also waste material, in carton simultaneously Standby paperboard be often atomized deadlocked glue, production process is complicated;And above-mentioned preparation method do not consider field from So natural enemy (Aranea, Formica fusca, ladybug and aphid) has a strong impact on the preventing and treating effect of trichogramma to the predation of egg parasitoid Really.The honeybee card using said method to make is single layer structure, and field egg parasitoid is under open environment, the most cruelly Dew and field, it is also easy to be subject to damaging of the irradiation drenched with rain with the sun;Honeybee prepared by the method blocks once making Indivisible so that it is can not the highly uniform trichogramma discharging equivalent accurately in unit are, and then can not Reach well to prevent the purpose of insect.
The present invention is directed to the deficiency that above-mentioned honeybee card exists, and the needs of trichogramma are thrown in field, it is provided that Yi Zhongjie Structure is simple, easy to operate and defence type trichogramma honeybee card being prone to factorial praluction and preparation method thereof.
Summary of the invention
Exempt from natural enemy infringement to overcome existing technology can not effectively prevent field from throwing in Trichogramma ovum, dislike Bad environmental nuisance and can not split and reach effective pest control purpose and honeybee card complicated process of preparation and materials wave The problem such as take, it is an object of the invention to provide a kind of defence type trichogramma honeybee card that can solve the problems referred to above and system thereof Preparation Method.
It is an object of the invention to be achieved through the following technical solutions:
A kind of defence type trichogramma honeybee card, including including ovum layer, porose cover layer, is characterized by ovum layer one Face is attached with Trichogramma ovum;Aperture is had on porose cover layer;Porose cover layer covers ovum layer and is attached with Trichogramma ovum one side, the two is glued together.
Further, described ovum layer and porose covering layer material are kraft paper.Kraft paper is readily obtained, and price is just Preferably, and solid, it is hardly damaged, and the honeybee card made can be made more durable.
Further, described Trichogramma ovum is attached to ovum layer by adhesive means.
Further, the gluing and described porose cover layer of described Trichogramma ovum is to pass through with the gluing of ovum layer
Flour paste realizes.
Further, described flour paste is adjusted with warm water volume (ml) by a certain percentage by flour weight (g) System forms, and ratio can be 1:2,1:2.5,1:3,1:3.5,1:4,1:4.5,1:5,1:5.5 etc., Qi Zhongyou The flour weight (g) of choosing is 1:3.5 with warm water volume (ml) ratio, and the temperature of described warm water is preferably 70 DEG C.
Further, described ovum layer and porose cover layer can arrange difformity, circular, oval, square Deng, the most square, arrange different size of square further, wherein preferably size is 21cm × 29.7cm Square structure.
Further, described ovum layer is that subregion ovum layer and is divided into territory, different districts, be preferably divided into 2-100 different Zonule, various different partitioned mode can be set, such as the modes such as 4 × 5,6 × 7,8 × 9,7 × 8, 56 zonules that wherein size of preferably 7 × 8 is identical, each two is provided with junction between zonule.This Selection can be used with the ovum layer that makes of maximum possible, and the most do not affect with porose cover layer is the most gluing.
Further, the rectangle of 1.5cm × 2.5cm sized by described each zonule, this structure can So that ovum layer effectively divide equally.
Further, described porose supratectal each zonule arranges different number of aperture, be preferably it In the aperture of 2 × 3,2 × 4,2 × 5 modes is perfectly even set.
Further, the porose zonule of described porose cover layer and described ovum layer ovum zonule corresponding. Described aperture size is configured such that trichogramma adult can pass through, and wherein preferably aperture is dimensioned to greatly diameter 1mm.Application the most in the wild is that in can making ovum layer zonule, the adult trichogramma that sprouts wings passes through cover layer The aperture of upper setting leaves honeybee card, carries out parasitism to field, reaches the purpose of Pest control.
Further, described ovum layer and porose cover layer are glued together by the junction of each zonule on it, By junction, so make each zonule separate, form the region of a relative closure, make honeybee card more firm Gu, it is not easy to by external environmental interference.Required size can also be cut into as required in junction.
Further, described honeybee card can be cut to identical little of size in the gluing junction of each zonule Honeybee blocks, the similar number of the upper egg parasitoid of each chalcid fly card, during by chalcid fly card application with wild environment, makes unit plane The trichogramma quantity of sprouting wings in Ji is more or less the same, and reaches to stablize the average purpose preventing insect.
The present invention further discloses the preparation method of a kind of defence type trichogramma honeybee card, comprise the following steps:
1) get out Trichogramma ovum, be modulated into flour paste with flour weight and warm water;
2) porose cover layer is holed, carry out gluing facing to ovum layer;
3) egg parasitoid is sticked at equably band glue on ovum layer;
4) the junction gluing to the porose cover layer after boring;
5) hole and band glue porose cover layer and gluing good egg parasitoid ovum layer be bonded together, obtain One big defence type trichogramma honeybee card.
Preferably, the preparation method of described defence type trichogramma honeybee card, comprise the following steps:
1) getting out the gelechiid ova of Trichogramma, flour weight (g) and warm water volume (ml) are 1:3.5 The flour paste of ratio;
2) hole uniformly on each zonule of porose cover layer, subregion ovum layer and carries out gluing;
3) on the workbench of cleaning, the subregion that egg parasitoid sticks at band glue equably on each zonule of ovum layer, Make subregion ovum layer the most gently lift and vibrate gently, make not glue steady or unnecessary egg parasitoid and fall, To reuse;
4) by the junction gluing of hole-drilled porose cover layer;
5) punching and the porose cover layer of band glue ovum layer with the subregion of gluing good egg parasitoid and overlapped, then Moulding makes at double-layer structure reconnection viscous steady, just obtains a big defence type trichogramma honeybee card;
6) with paper knife, big defence type trichogramma honeybee is stuck in junction and is cut into several chalcid flies card, be available for field Release, can save backup simultaneously.
Further, subregion ovum layer and is provided with 56 zonules of 7 × 8 subregions.
Further, hole uniformly with on each zonule of porose cover layer, the most a diameter of The fine needle of 1mm.
During use, directly take chalcid fly card or be taken under appropriate temperature conditions the chalcid fly card preserved, according to unit are Required honeybee card quantity is uniformly arranged a number of chalcid fly card in field, treats that the egg parasitoid on chalcid fly card turns into red After eye honeybee leaves chalcid fly card, trichogramma carries out ovipositing hosts on insect ovum, absorbs insect after trichogramma egg hatching The nutrition of worm's ovum, to reach to kill the purpose of insect.
Compared with prior art, the invention have effective effect that: cover subregion by porose cover layer and ovum layer, By covering protection egg parasitoid, so make egg parasitoid from or alleviate the injury drenched with rain with Exposure to Sunlight, and at cover layer The aperture of upper setting can make trichogramma get out;Gluing in the junction of each zonule by two-layer, it is each little Region all forms the environment of a relative closure, and then prevents field other natural enemies (Aranea, Formica fusca, wooden dipper Worm and aphid) predation of egg parasitoid is had a strong impact on the prevention effect of trichogramma;And honeybee card is cut to difference Chalcid fly card, convenient during use, be that in unit are, the burst size of trichogramma is more uniform.This preparation side Can effectively avoid waste during method preparation, also not have the phenomenon that gluing is irregular, and production operation is simple Single, rapid, convenient, substantially increase the make efficiency of honeybee card, be provided that production 1600-2800 per hour Opening honeybee card, in field for Biological control, usable floor area, up to 160-280 mu, reaches to improve preparation honeybee card Efficiency.Preparation and used material easily reach, low price.
Accompanying drawing explanation
Fig. 1 is overall structure schematic diagram of the present invention;
Fig. 2 is side structure schematic diagram of the present invention;
Fig. 3 is small regional structure schematic diagram of the present invention;
In figure, 1, subregion ovum layer;2, porose cover layer;3, junction;4, zonule;5, aperture.
Detailed description of the invention
Embodiment 1 fabrication preparing experiment
One, material
Its material mainly includes the gelechiid ova of Trichogramma, honeybee paperboard, Semen Tritici aestivi flour and warm water, its main work Tool is fabrication template, template box and paper knife.
Two, method
2.1 batter viscose glue tests
2.1.1 viscous card test
By the flour water (1:2,1:2.5,1:3,1:3.5,1:4,1:4.5,1:5,1:5.5) of variable concentrations cattle Brush into the wide lines of 1cm on mulberry paper, then kraft paper doubling is overlapped, be allowed to bond, after waiting flour water dry, See variable concentrations to the fastness of sticky paper and persistency, each process in triplicate, is observed and keeps a record every day. Firmly the paper of tearing side, sees whether it scatters and measures its fastness.The length of standing time sees what it bonded Persistency.
2.1.2 viscous ovum test
Flour weight (g) and warm water volume (ml) (70 DEG C) are made into 1:2,1:2.5,1:3,1:3.5,1:4, 8 variable concentrations gradients of 1:4.5,1:5,1:5.5, after stirring, with brush pen by the flour of variable concentrations Water brushes on the kraft paper of 1.5 × 2.5 sizes, and main detection ovum is bonded at fastness and the persistency of paper, each place Reason in triplicate, observe once by every day.After blocking with force vibration honeybee, see if there is ovum grain and fall and fall quantity How much measure its fastness.Its persistency is measured by the length of standing time.
2.2 glue emergence test after ovum
In ready-made chalcid fly blocks, randomly draw 5 pieces, under 5 times of visuals field of microscope, watch the plumage of egg parasitoid Rate, and record its emergence situation.
The honeybee that goes out of 2.3 honeybee cards is tested
Randomly draw 5 ready-made chalcid fly cards to be loaded on respectively in sealed bag, after 10 days, check that trichogramma sprouts wings and Honeybee situation, and record it and go out honeybee situation.
Three, result
Viscous card test test shows, the flour water of 1:2,1:2.5,1:3, the 1:3.5 bonding fastness to kraft paper Preferably, 1:4,1:4.5 take second place, but bonding strength is the highest, Easy open.After placing 17, contrast finds 1:3, The persistency of the flour water of 1:3.5 is preferable.The flour water concentration of 1:5,1:5.5 concentration is the lowest can not bond doubling Kraft paper.For the flour water bonding honeybee card determining to use 1:3.5 concentration easy to use.
Viscous ovum test shows, the flour water bonding gelechiid ova effect of 8 Concentraton gradient is the best, and fastness is with persistently The equal Pass Test requirement of property.But for the consideration comprehensive easy to use tested, determine with the flour of 1:3.5 concentration Water bonding gelechiid ova grain.
Test test of sprouting wings after viscous ovum shows, the chalcid fly being covered ovum by flour water is blocked, and the highest eclosion rate is 98.4%, Minimum eclosion rate is 83.2%, and average eclosion rate is 94.8%, complies fully with technical scheme requirement.
Honeybee blocks honeybee result of the test and shows, most exceeds 1100 trichogrammas of honeybee, minimum 890, averages out honeybee 980 Head.
2 one kinds of defence type trichogramma honeybee card structures of embodiment
A kind of defence type trichogramma honeybee card, including including that subregion ovum layer 1, porose cover layer 2, its feature Ovum layer 1 with porose cover layer 2 for subregion is the paper that two-layer size is identical;Subregion ovum layer 1 and is divided into identical little Region 4, is attached with Trichogramma ovum in each zonule 4;It is divided into identical porose little on porose cover layer 2 Region 4;Porose cover layer 2 covers on ovum layer 1 with subregion, and both are glued together.Subregion ovum layer 1 It is kraft paper with porose cover layer 2 material.Subregion ovum layer 1 and is set to square with porose cover layer 2, and size is The square structure of 21cm × 29.7cm.Subregion identical 42 zonules 4 of size that ovum layer 1 is divided into 6 × 7, Junction 3 it is provided with between each two zonule 4.Sized by each zonule 4,1.5cm × 2.5cm's is rectangular Shape, is attached with Trichogramma ovum by adhesive means in each zonule 4.Porose cover layer 2 porose little Region 4 and ovum layer ovum zonule 4 corresponding, aperture 5 is dimensioned to greatly diameter 1.2mm.Ovum Layer and porose cover layer 2 are glued together, by junction 3 by the junction 3 of each zonule 4 on it Make each zonule 4 separate, form the region of a relative closure, make honeybee card more firm.Porose cover layer 6 apertures 5 of 2 × 3 modes are set on each zonule 4 on 2.Adhesive means is to be realized by flour paste , flour paste is modulated by a certain percentage with warm water volume (ml) by flour weight (g) and is formed, flour weight Amount (g) is 1:3 with warm water volume (ml) ratio, and the temperature of warm water is 70 DEG C.Honeybee card can be in each zonule 4 Gluing junction 3 be cut to size identical chalcid fly card.
During use, directly take chalcid fly card or be taken under appropriate temperature conditions the chalcid fly card preserved, according to unit are Required honeybee card quantity is uniformly arranged a number of chalcid fly card in field, treats that the egg parasitoid on chalcid fly card turns into red After eye honeybee leaves chalcid fly card, trichogramma carries out ovipositing hosts on insect ovum, absorbs insect after trichogramma egg hatching The nutrition of worm's ovum, to reach to kill the purpose of insect.
3 one kinds of defence type trichogramma honeybee card structures of embodiment
A kind of defence type trichogramma honeybee card, including including that subregion ovum layer 1, porose cover layer 2, is characterized by It is the paper that two-layer size is identical that subregion ovum layer 1 with porose cover layer 2;Subregion ovum layer 1 and is divided into same cells Territory 4, is attached with Trichogramma ovum in each zonule 4;Identical porose community it is divided on porose cover layer 2 Territory 4;Porose cover layer 2 covers on ovum layer 1 with subregion, and both are glued together.Subregion ovum layer 1 with Porose cover layer 2 material is kraft paper.Subregion ovum layer 1 and is set to square with porose cover layer 2, and size is The square structure of 21cm × 29.7cm.Subregion 56 that size that ovum layer 1 subregion set-up mode is 7 × 8 is identical Individual zonule 4, is provided with junction 3 between each two zonule 4.1.5cm sized by each zonule 4 × The rectangle of 2.5cm, is attached with Trichogramma ovum by adhesive means in each zonule 4.Porose covering Porose zonule 4 and the ovum layer of layer 2 ovum zonule 4 corresponding, aperture 5 is dimensioned to greatly diameter 1mm.Ovum layer glued together by the junction 3 of each zonule 4 on it with porose cover layer 2, pass through Junction makes each zonule 4 separate, and forms the region of a relative closure, makes honeybee card more firm.Porose 10 apertures 5 of 2 × 5 modes are set on each zonule 4 on cover layer 2.Adhesive means is by face Powder sticks with paste realization, and flour paste is modulated by a certain percentage with warm water volume (ml) by flour weight (g) and formed, Flour weight (g) and warm water volume (ml) ratio are 1:3.5, and the temperature of warm water is 70 DEG C.Honeybee card can be respectively The gluing junction 3 of zonule 4 is cut to the chalcid fly card that size is identical.
During use, directly take chalcid fly card or be taken under appropriate temperature conditions the chalcid fly card preserved, according to unit are Required honeybee card quantity is uniformly arranged a number of chalcid fly card in field, treats that the egg parasitoid on chalcid fly card turns into red After eye honeybee leaves chalcid fly card, trichogramma carries out ovipositing hosts on insect ovum, absorbs insect after trichogramma egg hatching The nutrition of worm's ovum, to reach to kill the purpose of insect.
The preparation method of 4 one kinds of defence type trichogramma honeybee cards of embodiment
1, material
Mainly include the gelechiid ova of Trichogramma, honeybee paperboard, Semen Tritici aestivi flour and warm water.
2, method
The preparation method of described defence type trichogramma honeybee card, comprises the following steps:
1) getting out the gelechiid ova of Trichogramma, flour weight (g) and warm water volume (ml) are 1:3.5's The flour paste of ratio;
2) hole uniformly on each zonule of porose cover layer with the fine needle of 1mm, the subregion to 7 × 8 Ovum layer and carry out gluing;
3) on the workbench of cleaning, the subregion that egg parasitoid sticks at band glue equably on each zonule of ovum layer, Make subregion ovum layer the most gently lift and vibrate gently, make not glue steady or unnecessary egg parasitoid and fall, To reuse;
4) by the junction gluing of hole-drilled porose cover layer;
5) punching and the porose cover layer of band glue ovum layer with the subregion of gluing good egg parasitoid and overlapped, then Moulding makes at double-layer structure reconnection viscous steady, just obtains a big defence type trichogramma honeybee card;
6) with paper knife, big defence type trichogramma honeybee is stuck in junction and is cut into 56 chalcid fly cards, be available for field Release, can save backup simultaneously.

Claims (8)

1. a defence type trichogramma honeybee card, including ovum layer, porose cover layer, is characterized by that ovum is laminated and is attached with Trichogramma ovum;Aperture is had on porose cover layer;Porose cover layer covers ovum layer and is attached with Trichogramma ovum one side, and the two is glued together;Described ovum layer is that subregion ovum layer, is divided into different number zonule;Described subregion and is provided with junction between each zonule of ovum layer;Described ovum layer and porose cover layer are glued together by the junction of its upper each zonule, by junction, make each zonule separate, the region of one relative closure of formation.
Defence type trichogramma honeybee the most according to claim 1 blocks, it is characterised in that described ovum layer and porose covering layer material are kraft paper.
Defence type trichogramma honeybee the most according to claim 1 blocks, it is characterised in that described Trichogramma ovum is attached to ovum layer by adhesive means.
Defence type trichogramma honeybee the most according to claim 3 blocks, it is characterised in that described adhesive means is realized by flour paste.
Defence type trichogramma honeybee the most according to claim 4 blocks, it is characterised in that described flour paste is formed with the ratio modulation that warm water volume is 1g:3.5ml by flour weight.
Defence type trichogramma honeybee the most according to claim 1 blocks, it is characterised in that described aperture size is configured such that trichogramma adult can pass through.
Defence type trichogramma honeybee the most according to claim 1 blocks, it is characterised in that honeybee card is cut to the chalcid fly card that size is identical by the junction between described each zonule.
8. a preparation method for defence type trichogramma honeybee card, comprises the following steps:
1) get out the gelechiid ova of Trichogramma, be modulated into flour paste with flour and warm water;
2) hole uniformly on each zonule of porose cover layer, subregion ovum layer and carries out gluing;
3) on the workbench of cleaning, the subregion that egg parasitoid sticks at band glue equably on each zonule of ovum layer, makes subregion ovum layer the most gently and lifts and vibrate gently, makes not glue steady or unnecessary egg parasitoid and falls, in order to reuses;
4) the junction gluing to the porose cover layer after boring;
5) hole and band glue porose cover layer and gluing good egg parasitoid ovum layer be bonded together, obtain a big defence type trichogramma honeybee card.
CN201410520150.5A 2014-09-30 Defence type trichogramma honeybee card and preparation method thereof Expired - Fee Related CN104222025B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201410520150.5A CN104222025B (en) 2014-09-30 Defence type trichogramma honeybee card and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201410520150.5A CN104222025B (en) 2014-09-30 Defence type trichogramma honeybee card and preparation method thereof

Publications (2)

Publication Number Publication Date
CN104222025A CN104222025A (en) 2014-12-24
CN104222025B true CN104222025B (en) 2017-01-04

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