CN104206363B - Defence type oophagous trichogrammae honeybee card preparation facilities and using method thereof - Google Patents

Defence type oophagous trichogrammae honeybee card preparation facilities and using method thereof Download PDF

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CN104206363B
CN104206363B CN201410522924.8A CN201410522924A CN104206363B CN 104206363 B CN104206363 B CN 104206363B CN 201410522924 A CN201410522924 A CN 201410522924A CN 104206363 B CN104206363 B CN 104206363B
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template
ovum
layer
preparation facilities
depression
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CN104206363A (en
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白树雄
太红坤
郑礼
张峰
刘峙
顾中量
王根权
王振营
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Institute of Plant Protection of Chinese Academy of Agricultural Sciences
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Institute of Plant Protection of Chinese Academy of Agricultural Sciences
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Abstract

A kind of defence type oophagous trichogrammae honeybee card preparation facilities, comprise the depression template of punching template, protruding template and the male cooperation with protruding template, it is characterized in that, described device also comprises top cover and box body, and top cover is placed on box body; Each template is respectively fixed on top cover, and each template size is identical, and the partitioned mode in template is also identical; Preparation facilities also comprises a glue box, and glue box internal diameter size is identical with template size; Box body quantity is 4, depression template 2; Each template is all divided into territory, different districts according to same way.Each template is in the capable continuous productive process of device, and operating efficiency is high, and each step that uses is not interfered mutually.Apparatus of the present invention structure is simple, and using method is easy, and the 1600-2800 that can produce per hour opens honeybee card, biological control is used in field, usable floor area can reach 160-280 mu, substantially increases the efficiency making honeybee card, to also overcome when current device uses the problems such as materials wastes.

Description

Defence type oophagous trichogrammae honeybee card preparation facilities and using method thereof
Technical field
The invention belongs to raising or the cultivation apparatus technical field of invertebrate, relate to a kind of defence type oophagous trichogrammae honeybee card preparation facilities and using method thereof more specifically.
Background technology
Oophagous trichogrammae Trichogrammatid, Hymenoptera (Hymennoptera) Trichogrammatidae (Trichogrammatidae) little parasitic wasp, espespecially Trichogramma spp (Trichogramma), adult is long less than 1 millimeter, wing resembles a pear in shape, tool list vein and spike echinid, attached joint 3 saves, obviously.Larva is parasitic in the ovum of moth class, is therefore the natural enemy that a class has value very much, can be used for carrying out biological control to crop pests.
Utilize oophagous trichogrammae carry out biological control needs a large amount of production oophagous trichogrammae and discharge in field, many employings at present make oophagous trichogrammae honeybee and block and the method being invested in field.Like this, how making honeybee card rapidly and efficiently becomes a key issue, current domestic oophagous trichogrammae production plant mainly adopts the method gluings such as manual hairbrush brush coating, manual double faced adhesive tape viscose glue, mimeograph gluing and air pressure glue spraying, then adheres to egg parasitoid, finally completes honeybee blocking and does.Hairbrush brush coating and manual double faced adhesive tape is time-consuming takes a lot of work, and hairbrush brush coating and mimeograph glue uneven, there is the problem of egg parasitoid skewness in honeybee paperboard.Air pressure glue spraying cost is higher, the atomization of glue and cause environmental pollution, and also waste material, the paperboard for subsequent use in carton is often atomized deadlocked glue simultaneously, and production process is complicated; And the honeybee adopting said apparatus and method to make blocks for single layer structure, under field egg parasitoid is in open environment, is easily drenched with rain and sun burn; And honeybee blocks and not easily splits once making, can not even trichogramma releases exactly in field, effective prevention and control can not be carried out to insect.Comprehensively above-mentioned, we need to make a kind of efficient device preparing honeybee card fast, prepare a kind of honeybee card that more can adapt to external environment.
The deficiency that fine needle of the present invention exists above-mentioned honeybee card preparation facilities and preparation honeybee card, and the needs of oophagous trichogrammae are thrown in field, there is provided that a kind of to make structure simple, easy to operate and be easy to defence type oophagous trichogrammae honeybee card preparation facilities and the defence type oophagous trichogrammae honeybee card of factorial praluction.
Summary of the invention
The Trichogramma ovum that can not effectively prevent field from throwing in overcome prior art is encroached on by natural enemy, adverse circumstances are damaged, not easily effective pest control is carried out in segmentation, the problems such as honeybee card complicated process of preparation and materials waste, the object of the invention is to provide a kind of defence type oophagous trichogrammae honeybee card preparation facilities that can solve the problem.
The object of the invention is to be achieved through the following technical solutions:
A set of defence type oophagous trichogrammae honeybee card preparation facilities, comprises the depression template of punching template, protruding template and the male cooperation with protruding template, it is characterized in that, hole with punching template to porose cover layer, then by depression template to porose cover layer gluing; Meanwhile by protruding template facing to the gluing of ovum layer, gluing after ovum layer adheres to Trichogramma ovum, by adhere to egg parasitoid ovum layer and the porose cover layer of perforated belt glue be bonded together.
Further, described preparation facilities also comprises a glue box, and glue box internal diameter size is not less than template, for the gluing of protruding template with depression template.Preferred glue box internal diameter size is identical with template size, facilitate protruding template and depression template get glue.
Further, described preparation facilities also comprise one with the identical pressure strap form for ovum layer and porose cover layer are bonded together of depression template.
Further, described preparation facilities also comprises box body and top cover, and box body is used for fixing paper card, and top cover matches with box body, is provided with handle above.Be preferably 4 rectangle box bodys, corresponding with paper card size, alignment when box body facilitates device to use, entity bottom described box body, a pair, top lateral edges is given prominence to, another offside hollow.Four box bodys are respectively used to different step: porose cover layer is placed in the first box body punching template and holes to it; To ovum to be placed in the second box body by protruding template its gluing; The porose cover layer of boring is placed in the 3rd box body depression template to its gluing; By the porose cover layer of above-mentioned perforated belt glue and adhere to egg parasitoid ovum be placed in the 4th box body and overlap, the moulding of pressure strap form is sticky steady.Each template, box body, top cover, all match, and can be different size, and preferred box body internal diameter size is 21cm × 29.7cm.
Further, described punching template, protruding template, depression template are fixed on below top cover, conveniently take.Preferably by being adhesively fixed on top cover, the mode more particularly by screw is fixed on top cover, replacing for convenience detach.
Further, described punching template distributes fine needle.Fine needle diameter is 1-1.2mm, wherein preferred 1.0mm, and fine needle is used for for the cover layer of honeybee card evenly pricks hole, very convenient, and fine needle uses the bundle hole of the formation oophagous trichogrammae that can ensure all to sprout wings to pass through.
Further, subregion in described punching template, protruding template, depression template.Preferred described punching template, protruding template, depression template are evenly divided into 2-100 zonule.Preferably evenly be divided into 56 zonules of 7 × 8 modes or 42 zonules of 6 × 7 modes.The each zonule of punching template can arrange different fine needle arrangement modes, wherein preferably evenly arranges the fine needle of 2 × 3,2 × 4,2 × 5 modes.
Further, different modes can be taked to carry out subregion above described protruding template, blockage 42 blockages that are outstanding or 6 × 7 modes being wherein preferably evenly provided with 7 × 8 modes are given prominence to, different size can be selected in blockage bottom surface, wherein preferably blockage size is bottom surface 1.5cm × 2.5cm, thickness range is 0.5-1cm, protruding template be used for card to honeybee ovum layer the gluing of ovum region, gluing is accurately quick.
Further, depression template is square wooden unit, wooden unit can arrange corresponding blockage in different ways, wherein preferably 7 × 8 mode, 56 pieces of blockages are evenly set, form hole, 56 depression sides, the large I in little Fang Dong bottom surface arranges different size, is wherein preferably 1.5cm × 2.5cm, side's hole depth bounds is 0.5-0.8cm, and arranges two depression templates; A depression template is used for the junction gluing of honeybee card, and another in junction rubber seal, is called as moulding template for last honeybee card double-layer structure.
The present invention further discloses a kind of using method of defence type oophagous trichogrammae honeybee card preparation facilities, specifically comprise the following steps:
1) with punching template, porose cover layer is holed;
2) use depression template to porose cover layer gluing, by protruding template facing to the gluing of ovum layer;
3) egg parasitoid is sticked at equably on the deadlocked ovum layer of band;
4) by adhere to egg parasitoid ovum layer be bonded together with band glue porose cover layer.
Further, concrete steps comprise:
1) flour and warm water are modulated into flour paste and are placed in glue box, to protruding template and the gluing of depression template;
2) porose cover layer is fixed in box body, with punching template, it is holed, obtain porose cover layer with holes;
3) use depression template to porose cover layer gluing with holes, by protruding template facing to the gluing of ovum layer;
4) egg parasitoid is sticked at equably on the deadlocked ovum layer of band;
5) by adhere to egg parasitoid ovum layer and the porose cover layer of perforated belt glue coincide together, be bonded together both making with pressure strap form.
Further, specifically comprise the following steps:
1) first get out the gelechiid ova of Trichogramma, flour weight (g) and warm water (ml) volume are the flour paste of the ratio of 1:3.5;
2) porose cover layer is fixed in the first box body, with punching template, it is holed, obtain porose cover layer with holes; On clean workbench, incite somebody to action ovum to be placed in the second box body by protruding template its gluing;
3) on clean workbench, incite somebody to action ovum to be placed in the second box body by protruding template its gluing, egg parasitoid is sticked at equably band glue on ovum layer, then lift gently and vibrate gently, make not glue steady or unnecessary egg parasitoid to fall, to reuse;
4) the porose cover layer of boring is placed in the 3rd box body depression template to its gluing;
5) by the porose cover layer of above-mentioned perforated belt glue and adhere to egg parasitoid ovum be placed in the 4th box body and overlap, the moulding of pressure strap form is sticky steady, just obtains a large defence type oophagous trichogrammae honeybee card.
The present invention further discloses defence type oophagous trichogrammae honeybee card prepared by a kind of honeybee card preparation facilities, comprise comprising ovum layer, porose cover layer, it is characterized by ovum layer is the paper that two-layer size is identical with porose cover layer; Ovum layer and be divided into territory, same cells, in each zonule, be attached with Trichogramma ovum; Porose cover layer is divided into identical porose zonule; Porose cover layer covers with on ovum layer, and two is glued together.
Further, ovum layer and porose covering layer material is brown paper.
Further, ovum layer and porose cover layer is set to square, can arrange different size, preferred size is the square structure of 21cm × 29.7cm.
Further, ovum layer subregion and different arrangement mode be set, preferred embodiment to be mode be 7 × 8 identical 56 zonules of size, be provided with junction between every two zonules.
Further, sized by each zonule, different size can be set to, be preferably the rectangle of 1.5cm × 2.5cm, in each zonule, be attached with Trichogramma ovum by adhesive means.
Further, porose tectal porose zonule and ovum layer ovum zonule corresponding, aperture size is set to diameter 1-1.2mm, and preferred size is 1mm.
Further, ovum layer and porose cover layer glued together by the junction of its upper each zonule, by junction, make each zonule be separated like this, the region of a formation relative closure, make honeybee card more firm.
Further, adhesive means is that the flour weight (g) realized by flour paste is modulated by a certain percentage with warm water volume (ml) and formed, preferred flour weight (g) and warm water volume (ml) are than being 1:3, and the temperature of warm water is preferably 70 DEG C.
Further, honeybee card can be cut to the identical chalcid fly card of size in the gluing junction of each zonule.
During use, get the chalcid fly preserved under the low temperature 6 DEG C of conditions card cut out, the chalcid fly card of some is set according to the uniform amount of honeybee card required in unit are, after until chalcid fly card on egg parasitoid sprout wings into oophagous trichogrammae leave chalcid fly card after, oophagous trichogrammae fine needle carries out ovipositing hosts to preventing the worm's ovum of insect, absorb the nutrition of the worm's ovum of insect after institute's spawning and hatching, reach the object of kill pests.
Compared with prior art, the beneficial effect of defence type oophagous trichogrammae honeybee card preparation facilities of the present invention is: structure is simple, easy and simple to handle, the 1600-2800 that can produce per hour opens honeybee card, and be used for biological control in field, usable floor area can reach 160-280 mu, substantially increase making honeybee and block large efficiency, this device procedure uses, and does not mutually interfere between step, therefore to also overcome when current device uses the problems such as materials wastes.
And the beneficial effect of the honeybee card that this device is produced is: can prevent other natural enemy of field (spider, ant, ladybug and aphid) from having a strong impact on the control efficiency of application oophagous trichogrammae to the predation of egg parasitoid; Make egg parasitoid from or alleviate the injury drenched with rain with Exposure to Sunlight; And honeybee card being cut to different chalcid fly cards, convenient during use, in unit are, the burst size of oophagous trichogrammae is more even.
Accompanying drawing explanation
Fig. 1 is apparatus of the present invention overall structure schematic diagram;
Fig. 2 is apparatus of the present invention punching template structural representation;
Fig. 3 is apparatus of the present invention embossed mould plate structure schematic diagram;
Fig. 4 is apparatus of the present invention depression formwork structure schematic diagram;
Fig. 5 is apparatus of the present invention glue box structural representation;
Fig. 6 is apparatus of the present invention using method flow chart;
Fig. 7 is that apparatus of the present invention prepare defence type oophagous trichogrammae honeybee card overall structure schematic diagram;
Fig. 8 is that apparatus of the present invention prepare defence type oophagous trichogrammae honeybee card side structure schematic diagram;
In figure, 1, box body; 2, top cover; 21, handle; 3, punching template; 31, fine needle; 4 protruding templates; 41, blockage; 5, cave in template; 51 hole, depression sides; 6, glue box; 71, ovum layer; 72, porose cover layer; 73, junction; 74, zonule; 75, aperture.
Embodiment
The blocking of embodiment 1 one kinds of defence type oophagous trichogrammae honeybees is for apparatus structure
A kind of defence type oophagous trichogrammae honeybee card preparation facilities, comprise box body 1, top cover 2, punching template 3, protruding template 4, depression template 5, it is characterized in that, top cover 2 is placed on box body 1; Each template is respectively fixed on top cover 2, and each template size is identical, and the partitioned mode in template is also identical; Preparation facilities also comprises a glue box 6, and glue box 6 internal diameter size is identical with template size; Box body 1 four, shape is square, bottom entity, and a pair, top lateral edges is given prominence to, another offside hollow, and box body 1 quantity is 4; Top cover 2 is square, and its size matches with box body 1 top hollow space, and top is provided with handle, and handle is screwed on top cover 2.Top cover 2 four belows are respectively equipped with the protruding template of 1 punching template 3,4, two depression template 5; Punching template 3 and protruding template 4 are fixed on top cover 2 by adhesive means, and 2 depression templates 5 are screwed on top cover 2.Each template, box body 1 top cover 2, all match, and size is 21cm × 29.7cm.Punching template 3 is evenly divided into 56 zonules 74 of 7 × 8 modes, and each zonule 74 arranges fine needle 31 arrangement mode of 2 × 3 modes, and fine needle 31 diameter is 1mm, and fine needle 31 evenly pricks hole for the cover layer 72 blocked for honeybee; Protruding template 4 is 56 blockages 41 arranging of equally distributed 7 × 8 modes above, and blockage 41 size is bottom surface 1.5cm × 2.5cm, and thickness is 0.5cm, protruding template 4 for block to honeybee ovum layer 71 the gluing of ovum region, gluing is accurately quick.Two depression templates 5 are square wooden unit, and wooden unit evenly arranges 7 × 8 holes, mode 56 pieces of depression sides 51, and bottom surface, little side hole 51 is 1.5cm × 2.5cm, and square hole 51 degree of depth is 0.5cm; Junction 73 gluing of template 5 for blocking to honeybee of a depression, another for last honeybee card double-layer structure in junction 73 rubber seal.
The blocking of embodiment 2 one kinds of defence type oophagous trichogrammae honeybees is for apparatus structure
A kind of defence type oophagous trichogrammae honeybee card preparation facilities, comprise box body 1, top cover 2, punching template 3, protruding template 4, depression template 5, it is characterized in that, top cover 2 is placed on box body 1; Each template is respectively fixed on top cover 2, and each template size is identical, and the partitioned mode in template is also identical; Preparation facilities also comprises a glue box 6, and glue box 6 internal diameter size is identical with template size; Box body 1 is square, bottom entity, and a pair, top lateral edges is given prominence to, another offside hollow, and box body 1 quantity is 4; Top cover 2 four, shape is square, and its size matches with box body 1 top hollow space, and top is provided with handle, and handle is screwed on top cover 2.Top cover 2 four belows are respectively equipped with the protruding template of 1 punching template 3,4, two depression template 5; 2 depression templates 5 are fixed on top cover 2 with protruding template 4 by adhesive means, and punching template 3 is screwed on top cover 2.Each template, box body 1 top cover 2, all match, and size is 21cm × 29.7cm.Punching template 3 is evenly divided into 42 zonules of 6 × 7 modes, and each zonule arranges fine needle 31 arrangement mode of 2 × 5 modes, and fine needle 31 diameter is 1.2mm, and fine needle 31 evenly pricks hole for the cover layer 72 blocked for honeybee; Protruding template 4 is 42 blockages 41 arranging of equally distributed 6 × 7 modes above, and blockage 41 size is bottom surface 1.5cm × 2.5cm, and thickness is 0.8cm, protruding template 4 for block to honeybee ovum layer 71 the gluing of ovum region, gluing is accurately quick.Two depression templates 5 are square wooden unit, and wooden unit evenly arranges 6 × 7 holes, mode 42 pieces of depression sides 51, and bottom surface, little side hole 51 is 1.5cm × 2.5cm, and square hole 51 degree of depth is 0.5cm; Junction 73 gluing of template 5 for blocking to honeybee of a depression, another for last honeybee card double-layer structure in junction 73 rubber seal.
Preparing experiment before embodiment 3 honeybee card device uses
One, material
Its material mainly comprises the gelechiid ova of Trichogramma, honeybee paperboard, wheat flour and warm water, and its main tool is each template (punching template 3, protruding template 4,2 depression template 5), four box bodys 1 and paper knife.
Two, method
2.1 batter viscose glue tests
2.1.1 sticky card test
By flour water (1:2,1:2.5, the 1:3 of variable concentrations, 1:3.5,1:4,1:4.5,1:5, on brown paper, 1:5.5) brush into the wide lines of 1cm, then brown paper doubling is overlapped, make it bonding, Deng flour water dry after, see that variable concentrations is to the fastness of sticky paper and persistence, each process in triplicate, is observed and keeps a record every day.Firmly the paper of tearing side, sees whether it scatters to measure its fastness.The length of standing time sees its persistence bonded.
2.1.2 sticky ovum test
Flour weight (g) and warm water volume (ml) (70 DEG C) are made into 1:2,1:2.5,1:3,1:3.5,1:4,1:4.5,1:5,8 variable concentrations gradients of 1:5.5, after stirring, brush on the brown paper of 1.5cm × 2.5cm size with writing brush by the flour water of variable concentrations, main detection ovum is bonded at fastness and the persistence of paper, in triplicate, every day observes once in each process.With force vibration honeybee card after, see if there is ovum grain fall and fall quantity number to measure its fastness.Its persistence is measured by the length of standing time.
Sprout wings after 2.2 sticky ovum and test
In ready-made chalcid fly card, randomly draw 5 pieces, under microscope 5 times of visuals field, watch the eclosion rate of egg parasitoid, and record its emergence situation.
The honeybee that goes out of 2.3 honeybee cards is tested
Randomly draw 5 ready-made chalcid flies cards to be loaded on respectively in sealed bag, after 10 days, check that oophagous trichogrammae sprouts wings and honeybee situation, and record it and go out honeybee situation.
Three, result
Sticky card test test shows, the flour water of 1:2,1:2.5,1:3,1:3.5 is better to the bonding fastness of brown paper, and 1:4,1:4.5 take second place, but bonding strength is not high, Easy open.After placing 17, contrast finds that the persistence of the flour water of 1:3,1:3.5 is better.The too low brown paper that can not bond doubling of flour water concentration of 1:5,1:5.5 concentration.The flour water bonding honeybee card using 1:3.5 concentration is determined in order to easy to use.
Sticky ovum test shows, the flour water bonding gelechiid ova effect of 8 concentration gradients is all good, fastness and the equal Pass Test requirement of persistence.But in order to the consideration comprehensive easy to use tested, determine the flour water bonding gelechiid ova grain by 1:3.5 concentration.
Test test of sprouting wings after sticky ovum shows, covered the chalcid fly card of ovum by flour water, the highest eclosion rate is 98.4%, and minimum eclosion rate is 83.2%, and average eclosion rate is 94.8%, meets technical scheme requirement completely.Honeybee blocks honeybee result of the test and shows, exceeds honeybee 1100 oophagous trichogrammaes most, minimum 890, on average goes out honeybee 980.
The using method of embodiment 4 one kinds of defence type oophagous trichogrammae honeybee card preparation facilities
The present invention further discloses a kind of using method of defence type oophagous trichogrammae honeybee card preparation facilities, specifically comprise the following steps:
1) gelechiid ova of Trichogramma is first got out, the flour paste of the ratio that flour weight (g) and warm water volume (ml) are 1:3.5;
2) porose cover layer 72 brown paper being put in the first box body 1, on each zonule 74 of porose cover layer 72, pricking hole with having the top cover 2 of punching template 3 uniformly;
3) on clean workbench, to ovum layer 71 brown paper is put in the second box body 1, each zonule 74 gluing of ovum layer 71 is given with the top cover 2 with protruding template 4, and obtain little blob of viscose (1.5cm × 2.5cm), after egg parasitoid sticked at equably band glue on each zonule 74 of ovum layer 71; Then make ovum layer 71 lift and vibrate gently gently, make not glue steady or unnecessary egg parasitoid and fall, to reuse;
4) hole-drilled porose cover layer 72 is put in the 3rd box body 1, gives junction 73 gluing of porose cover layer 72 with the top cover 2 with depression template 5;
5) punched and porose cover layer with glue 72 and gluing good egg parasitoid ovum layer 71 be put in the 4th box body 1, make double-layer structure sticky steady in junction 73 with another top cover 2 moulding with depression template 5, just obtain a large defence type oophagous trichogrammae honeybee card.
6) the large honeybee card scissors obtained is cut into chalcid fly card in junction.
According to said method, design and the defence type oophagous trichogrammae honeybee card that completed, as shown in Figure 7,8.
The defence type oophagous trichogrammae honeybee card preparation facilities that the present invention is prepared by said method, test test of sprouting wings after sticky ovum shows, is covered the chalcid fly card of ovum by flour water, the highest eclosion rate is 98.4%, minimum eclosion rate is 83.2%, and average eclosion rate is 94.8%, meets technical scheme requirement completely.So the defence type oophagous trichogrammae honeybee card preparation facilities prepared by the inventive method, may be used for field, can effective pest control.
The defence type oophagous trichogrammae honeybee card that embodiment 5 one kinds is prepared with defence type oophagous trichogrammae honeybee card preparation facilities
Defence type oophagous trichogrammae honeybee card prepared by a kind of honeybee card preparation facilities, comprises comprising ovum layer 71, porose cover layer 72, and it is characterized by ovum layer 71 with porose cover layer 72 is the identical brown paper of two-layer size; Porose cover layer 72 covers with on ovum layer 71, and two is glued together; Ovum layer 71 square with porose cover layer 72 shape, size is 21cm × 29.7cm.Identical 56 zonules 74 of size that ovum layer 71 subregion set-up mode is 7 × 8, junction 73 is provided with between every two zonules 74, the rectangle of 1.5cm × 2.5cm sized by each zonule 74, is attached with Trichogramma ovum by adhesive means in each zonule 74.The porose zonule 74 of porose cover layer 72 and ovum layer ovum zonule 74 corresponding, the large size of aperture 75 is set to diameter 1.2mm.Ovum layer 71 glued together by the junction 73 of each zonule 74 on it with porose cover layer 72, by junction 73, make each zonule 74 be separated like this, form the region of a relative closure, honeybee is blocked more firm.Porose supratectal each zonule 4 is arranged 6 apertures 5 of 2 × 3 modes.Adhesive means is realized by flour paste, and flour weight (g) and warm water volume (ml) are than being 1:3, and the temperature of warm water is 70 DEG C.Honeybee card can be cut to the identical chalcid fly card of 56 sizes in the gluing junction 73 of each zonule 74.
During use, get the chalcid fly preserved under the low temperature 6 DEG C of conditions card cut out, the chalcid fly card of some is set according to the uniform amount of honeybee card required in unit are, after until chalcid fly card on egg parasitoid sprout wings into oophagous trichogrammae leave chalcid fly card after, oophagous trichogrammae fine needle carries out ovipositing hosts to preventing the worm's ovum of insect, absorb the nutrition of the worm's ovum of insect after institute's spawning and hatching, reach the object of kill pests.
The defence type oophagous trichogrammae honeybee card that embodiment 6 one kinds is prepared with defence type oophagous trichogrammae honeybee card preparation facilities
Defence type oophagous trichogrammae honeybee card prepared by a kind of honeybee card preparation facilities, comprises comprising ovum layer 71, porose cover layer 72, and it is characterized by ovum layer 71 with porose cover layer 72 is the identical brown paper of two-layer size; Porose cover layer 72 covers with on ovum layer 71, and two is glued together; Ovum layer 71 square with porose cover layer 72 shape, size is 21cm × 29.7cm.Identical 42 zonules 74 of size that ovum layer 71 subregion set-up mode is 6 × 7, junction 73 is provided with between every two zonules 74, the rectangle of 1.5cm × 2.5cm sized by each zonule 74, is attached with Trichogramma ovum by adhesive means in each zonule 74.The porose zonule 74 of porose cover layer 72 and ovum layer ovum zonule 74 corresponding, the large size of aperture 75 is set to diameter 1.2mm.Ovum layer 71 glued together by the junction 73 of each zonule 74 on it with porose cover layer 72, by junction 73, make each zonule 74 be separated like this, form the region of a relative closure, honeybee is blocked more firm.Each zonule 74 on porose cover layer 72 is arranged 10 apertures 75 of 2 × 5 modes.Adhesive means is realized by flour paste, and flour weight (g) and warm water volume (ml) are than being 1:3, and the temperature of warm water is 70 DEG C.Honeybee card can be cut to the identical chalcid fly card of 42 sizes in the gluing junction 73 of each zonule 74.
During use, get the chalcid fly preserved under the low temperature 6 DEG C of conditions card cut out, the chalcid fly card of some is set according to the uniform amount of honeybee card required in unit are, after until chalcid fly card on egg parasitoid sprout wings into oophagous trichogrammae leave chalcid fly card after, oophagous trichogrammae fine needle carries out ovipositing hosts to preventing the worm's ovum of insect, absorb the nutrition of the worm's ovum of insect after institute's spawning and hatching, reach the object of kill pests.

Claims (9)

1. a set of defence type oophagous trichogrammae honeybee card preparation facilities, comprises the depression template of punching template, protruding template and the male cooperation with protruding template, it is characterized in that, hole with punching template to porose cover layer, then by depression template to porose cover layer gluing; Meanwhile by protruding template facing to the gluing of ovum layer, gluing after ovum layer adheres to Trichogramma ovum, incite somebody to action ovum layer and porose cover layer be bonded together;
Described preparation facilities also comprises a pressure strap form for ovum layer and porose cover layer be bonded together identical with depression template.
2. defence type oophagous trichogrammae honeybee card preparation facilities according to claim 1, it is characterized in that, described preparation facilities also comprises a glue box, and glue box internal diameter size is not less than template, for the gluing of protruding template with depression template.
3. defence type oophagous trichogrammae honeybee card preparation facilities according to claim 1, it is characterized in that, described preparation facilities also comprises box body and top cover, and box body is used for fixing paper card, and top cover matches with box body, is provided with handle above.
4. defence type oophagous trichogrammae honeybee card preparation facilities according to claim 3, is characterized in that, described punching template, protruding template, depression template are fixed on below top cover.
5. defence type oophagous trichogrammae honeybee card preparation facilities according to claim 1, it is characterized in that, described punching template distributes fine needle.
6. defence type oophagous trichogrammae honeybee card preparation facilities according to claim 1, is characterized in that, subregion in described punching template, protruding template, depression template.
7. defence type oophagous trichogrammae honeybee card preparation facilities according to claim 6, is characterized in that, described punching template, protruding template, depression template are evenly divided into 2-100 zonule.
8. the using method of the defence type oophagous trichogrammae honeybee card preparation facilities described in claim 1 to 7 any one, specifically comprises the following steps:
1) with punching template, porose cover layer is holed;
2) use depression template to porose cover layer gluing, by protruding template facing to the gluing of ovum layer;
3) egg parasitoid is sticked at equably on the deadlocked ovum layer of band;
4) by adhere to egg parasitoid ovum layer be bonded together with band glue porose cover layer.
9. using method according to claim 8, is characterized in that, concrete steps comprise:
1) flour and warm water are modulated into flour paste and are placed in glue box, to protruding template and the gluing of depression template;
2) porose cover layer is fixed in box body, with punching template, it is holed, obtain porose cover layer with holes;
3) use depression template to porose cover layer gluing with holes, by protruding template facing to the gluing of ovum layer;
4) egg parasitoid is sticked at equably on the deadlocked ovum layer of band;
5) by adhere to egg parasitoid ovum layer and the porose cover layer of perforated belt glue coincide together, be bonded together both making with pressure strap form.
CN201410522924.8A 2014-09-30 2014-09-30 Defence type oophagous trichogrammae honeybee card preparation facilities and using method thereof Active CN104206363B (en)

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CN108552121B (en) * 2018-07-03 2023-09-29 安徽国援智能科技有限公司 Rotary bee card manufacturing device
CN110839595B (en) * 2019-10-30 2021-09-14 山东省农业科学院植物保护研究所 Trichogrammatid bee card packing machine
CN115486420B (en) * 2022-10-25 2023-06-13 重庆师范大学 Bee nondestructive egg queen breeding method

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WO2003071563A1 (en) * 2002-02-19 2003-08-28 Cellergy Ltd. Electrochemical capacitor and method for its preparation
CN1279809C (en) * 2005-01-26 2006-10-18 北京市农林科学院 Artificial breeding and producing method for trichogramma
JP2009503840A (en) * 2005-07-27 2009-01-29 セラジー リミテッド Multilayer electrochemical energy storage device and manufacturing method thereof
BRMU8701832Y1 (en) * 2007-09-04 2016-05-31 Danilo Scacalossi Pedrazzoli constructive arrangement applied to a carton for conditioning biological agents
CN202514445U (en) * 2012-03-30 2012-11-07 吉林农业大学 Small-egg trichogramma release device
CN202524979U (en) * 2012-05-09 2012-11-14 滨州学院 Dastarcus helophoroides egg release card
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