CN104221976A - Method for waterless keep-alive transportation by narcotizing tilapia mossambica with low-concentration carbon dioxide - Google Patents
Method for waterless keep-alive transportation by narcotizing tilapia mossambica with low-concentration carbon dioxide Download PDFInfo
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- CN104221976A CN104221976A CN201410548444.9A CN201410548444A CN104221976A CN 104221976 A CN104221976 A CN 104221976A CN 201410548444 A CN201410548444 A CN 201410548444A CN 104221976 A CN104221976 A CN 104221976A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention relates to a method for waterless keep-alive transportation by narcotizing tilapia mossambica with low-concentration carbon dioxide and belongs to the technical field of living fish waterless transportation. According to the method, the carbon dioxide serves as the anesthetic of the tilapia mossambica, the influences of the concentration of the carbon dioxide, the temperature of narcotizing liquid, the narcotizing time, the keep-alive temperature, oxygenation and other parameters on the waterless keep-alive time of the tilapia mossambica are studied, and the optimal tilapia mossambica waterless keep-alive transportation parameters are determined. After the method is applied, the tilapia mossambica can be kept alive for four to five hours, and the survival rate is 100 percent. According to the method, the transportation efficiency is improved, the transportation cost is reduced, and meanwhile the transported tilapia mossambica does not have the potential safety hazard of medicine residues.
Description
Technical field
The present invention relates to the waterless keep-alive transportation resources of Tilapia mossambica, be specifically related to a kind of waterless keep-alive transportation resources adopting low concentration of carbon dioxide to anaesthetize Tilapia mossambica, belong to live fish anhydrous technical field of transportation.
Background technology
Tilapia mossambica spinous dorsal portion is flourishing, and when being subject to environmental stimuli, the hard fin ray in back can be holded up, and has aggressiveness, easily damages people and fish body, is unfavorable for Tilapia mossambica transport, adopts the practical operations such as ovum.Because Tilapia mossambica belongs to tropic fishes, under the impact of weather and temperature, the cultivation of Tilapia mossambica presents the few phenomenon of Nan Duobei in CONTINENTAL AREA OF CHINA.Current China Tilapia mossambica product except marketing fresh, mainly freezing full fish, freeze Tilapia Fillet and be processed as master, fresh fish sheet quantity is also relatively less.
Fresh and alive fish is without bacteriological infection, and safety is high, economic worth and market potential huge, fresh and alive fish can retain the original local flavor of fish and nutritive value to greatest extent, and the personal value exceeds more than one times than chilled fish.On Transportaion of Live mode is the most effective means keeping the best freshness of aquatic products, meet consumer demand, also can improve the economic benefit of enterprise simultaneously.Therefore the fresh and alive Tilapia mossambica of a kind of transport of selection, and the transportation of live fish method of raising conevying efficiency, reduction cost of transportation is particularly important when transporting.
The auxiliary transportation of live fish method of anesthesia can make fish enter the state of similar dormancy, and irritant reaction is blunt to external world, reduces the metabolic rate of fish, thus improves conevying efficiency, reduces cost of transportation.Current anaesthesia mainly comprises the non-chemically drug anesthesia method such as chemicals anaesthesia and hypothermic anesthesia, electric shock anesthesia and acupuncture anesthesia.Chemicals anaesthetic mainly comprises MS-222, eugenol procaine hydrochloride, carbonic acid, carbonic acid gas etc.After chemistry anaesthetic anesthesia fish body, can be residual in fish body, there is potential safety hazard.Carbonic acid gas, compared with other chemical anaesthetic, has the advantage of self, and carbonic acid gas is as fish anaesthetic, and can not remain in fish body, the Tilapia mossambica through carbon dioxide treatment does not have the paracmastic regulation of medicine, can directly commercially sell.
At present, the fresh and alive transportation resources of some fish has mainly adopted water transport defeated, and has water transport defeated existence transport water body volume large, and fish body useful load is few, and fish body metabolism degree is large, and oxygen-supplying amount is large, water treatment difficulty, high in cost of production problems.Waterless keep-alive transport is the once innovation to Traditional Transportation method, and waterless keep-alive transport can increase traffic density, extends haulage time; reducing cost of transportation, without the need to processing transport water quality, simplifying transportation management; reduce environmental pollution, avoid seawater corrosion, there is good development prospect.
Summary of the invention
The object of this invention is to provide a kind of waterless keep-alive transportation resources adopting low concentration of carbon dioxide to anaesthetize Tilapia mossambica, the method adopts carbonic acid gas as fish anaesthetic, and avoid the problem that chemicals is residual in fish body, conevying efficiency is high, cost of transportation is low.The present invention studies the conditions such as gas concentration lwevel, anesthesia liquid water temperature, anesthesia duration, keep-alive temperature, oxygen supply to the impact of Tilapia mossambica keep-alive time, determines best keepalive parameter, makes the waterless keep-alive time of Tilapia mossambica the longest.
For solving the problem, the technical solution adopted in the present invention is: a kind of waterless keep-alive transportation resources adopting carbon dioxide narcosis Tilapia mossambica, the steps include:
(1) fresh and alive Tilapia mossambica is stopped eating and support 20-24 hour temporarily;
(2) in fishbowl, fresh water is injected, the temperature regulating water in fish jar is 25 DEG C, Xiang Shuizhong passes into carbon dioxide and oxygen, period uses Carbon dioxide gas-sensing probe to measure the concentration of carbonic acid gas in the aqueous solution, obtains by the size of regulation of carbon dioxide and oxygen flow and duration of ventilation the aqueous solution that gas concentration lwevel is 0.27-0.60mg/L;
(3) fishing net is used to be pulled out from culturing pool by the Tilapia mossambica supported temporarily that stops eating, put into arbon dioxide solution, until Tilapia mossambica falls or sinks to bottom fish jar or bubble through the water column, gill dynamic frequency declines, shallow breathing or stopping, now completing the anesthesia of Tilapia mossambica;
(4) narcose Tilapia mossambica will be in pull out from the carbon dioxide narcosis aqueous solution, then put it in oxygenation bag, oxygen is filled with in oxygenation bag, the oxygenation bag that narcosis Tilapia mossambica is housed is put into insulating box and simulates anhydrous transport, in the process of the anhydrous transport of simulation, control keep-alive temperature at 20-25 DEG C;
(5), after Tilapia mossambica simulation waterless keep-alive transport 4-5h, Tilapia mossambica is released from oxygenation bag, puts it in advance in the fresh water of oxygenation, can recover in Tilapia mossambica 5min, recover active immediately.
the invention has the beneficial effects as follows:
The present invention adopts carbonic acid gas as fish anaesthetic, and carbonic acid gas, as the chemical anaesthetic of one, can not remain, without the paracmastic regulation of medicine, also not have injury for operator and environment in fish body.This method adopts waterless keep-alive transport simultaneously, can solve the problems such as water transport defeated macrura reevesii body useful load is few, oxygen demand is large, water treatment is difficult.The present invention, by the impact on the Tilapia mossambica keep-alive time such as research arbon dioxide solution concentration, water temperature, anesthesia duration, keep-alive temperature, determines best Tilapia mossambica waterless keep-alive shipping parameters.The present invention can improve conevying efficiency, saves cost of transportation, for transportation of live fish provides new method.
Specific embodiment
Be further elaborated the present invention below in conjunction with embodiment, these embodiments, only for illustration of the present invention, do not limit the scope of the invention.
Embodiment 1
Adopt a waterless keep-alive transportation resources for carbon dioxide narcosis Tilapia mossambica, comprise the following steps:
(1) select physical health, without wound, Tilapia mossambica that scale is complete, the Tilapia mossambica selected stopped eating and supports 24h temporarily;
(2) in fishbowl, fresh water is injected, the temperature regulating water in fish jar is 25 DEG C, Xiang Shuizhong passes into carbon dioxide and oxygen, period uses Carbon dioxide gas-sensing probe to measure the concentration of carbonic acid gas in the aqueous solution, obtains by the size of regulation of carbon dioxide and oxygen flow and duration of ventilation the aqueous solution that gas concentration lwevel is 0.27mg/L;
(3) use fishing net by temporarily support after Tilapia mossambica pull out from culturing pool, put into anesthesia fish jar, anesthesia duration is 13min;
(4) will be in after narcose Tilapia mossambica is pulled out from anesthesia bathtub and put into oxygenation bag, oxygen is filled with in oxygenation bag, the oxygenation bag that narcosis Tilapia mossambica is housed is put into insulating box and simulates dried up transport, in the process of the dried up transport of simulation, control keep-alive temperature remain on 25 DEG C;
(5), after Tilapia mossambica simulation waterless keep-alive transport 4h, released from oxygenation bag by Tilapia mossambica, put it in advance in the fresh water of oxygenation, can recover in Tilapia mossambica 5min, anabiosis rate is 100%, recovers active immediately.
Embodiment 2
Adopt a waterless keep-alive transportation resources for carbon dioxide narcosis Tilapia mossambica, comprise the following steps:
(1) select physical health, without wound, Tilapia mossambica that scale is complete, the Tilapia mossambica selected stopped eating and supports 20h temporarily;
(2) in fishbowl, fresh water is injected, the temperature regulating water in fish jar is 25 DEG C, Xiang Shuizhong passes into carbon dioxide and oxygen, period uses Carbon dioxide gas-sensing probe to measure the concentration of carbonic acid gas in the aqueous solution, obtains by the size of regulation of carbon dioxide and oxygen flow and duration of ventilation the aqueous solution that gas concentration lwevel is 0.41mg/L;
(3) use fishing net by temporarily support after Tilapia mossambica pull out from culturing pool, put into anesthesia fish jar, anesthesia duration is 10min;
(4) will be in after narcose Tilapia mossambica is pulled out from anesthesia bathtub and put into oxygenation bag, oxygen is filled with in oxygenation bag, the oxygenation bag that narcosis Tilapia mossambica is housed is put into insulating box and simulates dried up transport, in the process of the dried up transport of simulation, control keep-alive temperature remain on 20 DEG C;
(5), after Tilapia mossambica simulation waterless keep-alive transport 5h, released from oxygenation bag by Tilapia mossambica, put it in advance in the fresh water of oxygenation, can recover in Tilapia mossambica 5min, anabiosis rate is 100%, recovers active immediately.
Embodiment 3
Adopt a waterless keep-alive transportation resources for carbon dioxide narcosis Tilapia mossambica, comprise the following steps:
(1) select physical health, without wound, Tilapia mossambica that scale is complete, the Tilapia mossambica selected stopped eating and supports 22h temporarily;
(2) in fishbowl, fresh water is injected, the temperature regulating water in fish jar is 25 DEG C, Xiang Shuizhong passes into carbon dioxide and oxygen, period uses Carbon dioxide gas-sensing probe to measure the concentration of carbonic acid gas in the aqueous solution, obtains by the size of regulation of carbon dioxide and oxygen flow and duration of ventilation the aqueous solution that gas concentration lwevel is 0.60 mg/L;
(3) use fishing net by temporarily support after Tilapia mossambica pull out from culturing pool, put into anesthesia fish jar, anesthesia duration is 7min;
(4) will be in after narcose Tilapia mossambica is pulled out from anesthesia bathtub and put into oxygenation bag, oxygen is filled with in oxygenation bag, the oxygenation bag that narcosis Tilapia mossambica is housed is put into insulating box and simulates anhydrous transport, the temperature controlling fish in the process of the dried up transport of simulation remains on 20 DEG C;
(5), after Tilapia mossambica simulation waterless keep-alive transport 4h, released from oxygenation bag by Tilapia mossambica, put it in advance in the fresh water of oxygenation, can recover in Tilapia mossambica 5min, anabiosis rate is 100%, recovers active immediately.
Claims (4)
1. adopt low concentration of carbon dioxide to anaesthetize a waterless keep-alive transportation resources for Tilapia mossambica, it is characterized in that, comprise the following steps:
(1) fresh and alive Tilapia mossambica is stopped eating and support 20-24h temporarily;
(2) in fishbowl, running water is injected, Xiang Shuizhong passes into carbon dioxide and oxygen, period uses dioxy carbon gas sensing electrode to measure the concentration of carbonic acid gas in the aqueous solution, obtains the low concentration of carbon dioxide aqueous solution by the size of regulation of carbon dioxide and oxygen flow and duration of ventilation;
(3) fishing net is used to be pulled out from culturing pool by the Tilapia mossambica supported temporarily that stops eating, put into arbon dioxide solution, until Tilapia mossambica falls or sinks to bottom fish jar or bubble through the water column, gill dynamic frequency declines, shallow breathing or stopping, now completing the anesthesia of Tilapia mossambica;
(4) narcose Tilapia mossambica will be in pull out from the carbon dioxide narcosis aqueous solution, and then put it in oxygenation bag, in oxygenation bag, be filled with oxygen, and the oxygenation bag that narcosis Tilapia mossambica is housed be put into insulating box and simulates dried up transport;
(5), after Tilapia mossambica simulation waterless keep-alive transport 4-5h, Tilapia mossambica is released from oxygenation bag, puts it in advance in the fresh water of oxygenation and recover.
2. a kind of waterless keep-alive transportation resources adopting low concentration of carbon dioxide to anaesthetize Tilapia mossambica according to claim 1, it is characterized in that, in described step (2), the concentration of described arbon dioxide solution is 0.27-0.60mg/L.
3. a kind of waterless keep-alive transportation resources adopting low concentration of carbon dioxide to anaesthetize Tilapia mossambica according to claim 1, it is characterized in that, in described step (3), anesthesia duration is 7-13min, and water temperature is 20-25 DEG C.
4. a kind of waterless keep-alive transportation resources adopting low concentration of carbon dioxide to anaesthetize Tilapia mossambica according to claim 1, it is characterized in that, in described step (4), the temperature of insulating box is 20-25 DEG C.
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Cited By (12)
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|---|---|---|---|---|
| CN104798701A (en) * | 2015-03-30 | 2015-07-29 | 朱佳杰 | Long-distance tilapia fry transportation method |
| CN105191833A (en) * | 2015-05-15 | 2015-12-30 | 上海海洋大学 | Method for keeping large number of freshwater fishes alive without water |
| CN105706983A (en) * | 2016-03-16 | 2016-06-29 | 上海海洋大学 | Waterless keep-alive transportation method of live shrimps |
| CN105961254A (en) * | 2016-06-12 | 2016-09-28 | 广西大学 | Method for waterlessly transporting tilapia mossambica in summer |
| CN106719196A (en) * | 2016-12-08 | 2017-05-31 | 广东海洋大学 | A kind of waterless keep-alive transportation resources of Environment of Litopenaeus vannamei Low |
| CN108419723A (en) * | 2018-03-20 | 2018-08-21 | 广东海洋大学 | A kind of carbon dioxide narcosis method of seawater grouper |
| CN108477031A (en) * | 2018-03-20 | 2018-09-04 | 广东海洋大学 | A kind of anhydrous transportation method using cold carbon dioxide narcosis seawater grouper |
| CN110192534A (en) * | 2019-06-17 | 2019-09-03 | 中国农业大学 | The optimization method and system in a kind of aquatic products waterless keep-alive transport temperature control section |
| CN111567476A (en) * | 2020-05-20 | 2020-08-25 | 山东青年政治学院 | Oxygen charging amount determination method and device |
| CN111616085A (en) * | 2020-06-05 | 2020-09-04 | 中国水产科学研究院渔业机械仪器研究所 | Electricity and carbon dioxide combined anesthesia method for Epinephelus fuscoguttatus |
| CN111616084A (en) * | 2020-06-05 | 2020-09-04 | 中国水产科学研究院渔业机械仪器研究所 | Keep-alive transportation method for anesthetizing epinephelus fuscoguttatus by using electricity and carbon dioxide in combined mode |
| CN114711172A (en) * | 2022-05-09 | 2022-07-08 | 渤海大学 | High-density keep-alive method for seawater groupers |
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104798701A (en) * | 2015-03-30 | 2015-07-29 | 朱佳杰 | Long-distance tilapia fry transportation method |
| CN105191833A (en) * | 2015-05-15 | 2015-12-30 | 上海海洋大学 | Method for keeping large number of freshwater fishes alive without water |
| CN105706983A (en) * | 2016-03-16 | 2016-06-29 | 上海海洋大学 | Waterless keep-alive transportation method of live shrimps |
| CN105961254A (en) * | 2016-06-12 | 2016-09-28 | 广西大学 | Method for waterlessly transporting tilapia mossambica in summer |
| CN106719196A (en) * | 2016-12-08 | 2017-05-31 | 广东海洋大学 | A kind of waterless keep-alive transportation resources of Environment of Litopenaeus vannamei Low |
| CN108477031A (en) * | 2018-03-20 | 2018-09-04 | 广东海洋大学 | A kind of anhydrous transportation method using cold carbon dioxide narcosis seawater grouper |
| CN108419723A (en) * | 2018-03-20 | 2018-08-21 | 广东海洋大学 | A kind of carbon dioxide narcosis method of seawater grouper |
| CN110192534A (en) * | 2019-06-17 | 2019-09-03 | 中国农业大学 | The optimization method and system in a kind of aquatic products waterless keep-alive transport temperature control section |
| CN111567476A (en) * | 2020-05-20 | 2020-08-25 | 山东青年政治学院 | Oxygen charging amount determination method and device |
| CN111567476B (en) * | 2020-05-20 | 2021-09-28 | 山东青年政治学院 | Oxygen charging amount determination method and device |
| CN111616085A (en) * | 2020-06-05 | 2020-09-04 | 中国水产科学研究院渔业机械仪器研究所 | Electricity and carbon dioxide combined anesthesia method for Epinephelus fuscoguttatus |
| CN111616084A (en) * | 2020-06-05 | 2020-09-04 | 中国水产科学研究院渔业机械仪器研究所 | Keep-alive transportation method for anesthetizing epinephelus fuscoguttatus by using electricity and carbon dioxide in combined mode |
| CN111616084B (en) * | 2020-06-05 | 2022-01-14 | 中国水产科学研究院渔业机械仪器研究所 | Keep-alive transportation method for anesthetizing epinephelus fuscoguttatus by using electricity and carbon dioxide in combined mode |
| CN114711172A (en) * | 2022-05-09 | 2022-07-08 | 渤海大学 | High-density keep-alive method for seawater groupers |
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Effective date of registration: 20141205 Address after: 524000 Guangdong city of Zhanjiang Province East Huguangyan Mazhang District of Guangdong Ocean University Applicant after: Guangdong Ocean University Applicant after: Guangdong Yujia Aquatic Food Co., Ltd. Address before: 524000 Guangdong city of Zhanjiang Province East Huguangyan Mazhang District of Guangdong Ocean University Applicant before: Guangdong Ocean University |
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