CN104221871A - Fast reproduction method for Vietnamese sophora root tissue culture - Google Patents
Fast reproduction method for Vietnamese sophora root tissue culture Download PDFInfo
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- CN104221871A CN104221871A CN201410540435.5A CN201410540435A CN104221871A CN 104221871 A CN104221871 A CN 104221871A CN 201410540435 A CN201410540435 A CN 201410540435A CN 104221871 A CN104221871 A CN 104221871A
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- bud
- sophora tonkinensis
- tonkinensis gapnep
- induction
- stem
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Abstract
The invention discloses a fast reproduction method for Vietnamese sophora root tissue culture. The fast reproduction method includes steps of obtaining sterile buds, inducing the buds, proliferating cluster buds, inducing rooting, performing acclimatization and transplant, and the like. According to the fast reproduction method for Vietnamese sophora root tissue culture, by optimizing the most suitable cultivation conditions of each culture phase, a fast reproduction system of Vietnamese sophora root tissue culture is improved, and a technical support is provided for large-scale cultivation and development.
Description
Technical field
The present invention relates to the quick-breeding method of sophora tonkinensis Gapnep tissue cultures, belong to plant technology field.
Background technology
Sophora tonkinensis Gapnep,
sophora tonkinensis Gagnep., pulse family, also known as sophora subprostrata, root of subprostrate sophora, shrub, winglike compound leaf alternate, column cap is put on an arrow growth pubescence.Pod beaded.The florescence 5-6 month, the fruit phase 7-8 month.Produce Guangxi, Guizhou, Yunnan.Be born in the tor in subtropics or temperate zone or the spinney of Limestone Mountain; Height above sea level 1000-2000 rice.North Vietnam also has distribution.Domesticly be distributed in Guizhou Province, osmanthus, Yunnan, main product Guangxi.Be born in the Limestone Mountain of height above sea level 1000-2000 or rock seam.Root is more sturdy, and containing matrine alkaloid, can be used as medicine, have clearing heat and detoxicating, the effect of anti-inflammatory analgetic, propagation method is natural propagation mainly, gathers in field.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of method for quickly breeding of sophora tonkinensis Gapnep, the inventive method is by the optimization to each cultivation stage optimal culture conditions, the perfect rapid propagation system of sophora tonkinensis Gapnep tissue cultures, provides technical support for cultivating and open utilization on a large scale.
Technical problem to be solved by this invention is realized by following scheme:
Get sophora tonkinensis Gapnep stem with bud, surface contaminants removed by hairbrush, running water 3h, on superclean bench 70% ethanol postincubation 22s, aseptic water washing 3 times, the clorox process 13min of 0.2%, aseptic water washing 4 times, the sophora tonkinensis Gapnep stem with bud access HE+6-BA5mg/L+2 disinfected, the induction of axillalry bud is carried out in 4-D0.02mg/L medium, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 2200lx, light application time 13h/d, temperature 25 DEG C, the axillalry bud derived is put into medium HE+6-BA1.5mg/L+2ip0.5mg/L and is carried out squamous subculture, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 3000lx, light application time 15h/d, temperature 25 DEG C, bud seedling access medium 1/4MS+IBA0.7mg/L+GA after propagation
3root induction is carried out, additional saccharose 20g/L, agar 5.8g/L in 1mg/L, Ph6.0, illumination 12000lx, light application time 18h/d, temperature 25 DEG C, in units of every clump of 6 strains, cleans medium, 20s is dipped in the liquor potassic permanganate of 0.5%, be transplanted to fill with 0.5% disinfecting solution of potassium permanganate Nutrition Soil nutritive cube in, matrix is rural area soil: vermiculite=2:1, temperature 25 DEG C, relative air humidity 82%, sprays water 3 every day.Addition of gibberellin in the induction of seedling stem section, the induction of stem section can be promoted, after one month, add up survival rate.
The sophora tonkinensis Gapnep rooting rate adopting the present invention to prepare is high, and the cycle is short, and output is large, pollutes little, is beneficial to implant mass.
Below in conjunction with embodiment, the present invention is further elaborated, but the scope of protection of present invention is not limited to following embodiments.
Embodiment
Embodiment 1
Get sophora tonkinensis Gapnep stem with bud, surface contaminants removed by hairbrush, running water 3h, on superclean bench 70% ethanol postincubation 22s, aseptic water washing 3 times, the clorox process 13min of 0.2%, aseptic water washing 4 times, the sophora tonkinensis Gapnep stem with bud access HE+6-BA5mg/L+2 disinfected, the induction of axillalry bud is carried out in 4-D0.02mg/L medium, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 2200lx, light application time 13h/d, temperature 25 DEG C, the axillalry bud derived is put into medium HE+6-BA1.5mg/L+2ip0.5mg/L and is carried out squamous subculture, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 3000lx, light application time 15h/d, temperature 25 DEG C, bud seedling access medium 1/4MS+IBA0.7mg/L+GA after propagation
3root induction is carried out, additional saccharose 20g/L, agar 5.8g/L in 1mg/L, Ph6.0, illumination 12000lx, light application time 18h/d, temperature 25 DEG C, in units of every clump of 6 strains, cleans medium, 20s is dipped in the liquor potassic permanganate of 0.5%, be transplanted to fill with 0.5% disinfecting solution of potassium permanganate Nutrition Soil nutritive cube in, matrix is rural area soil: vermiculite=2:1, temperature 25 DEG C, relative air humidity 82%, sprays water 3 every day.Addition of gibberellin in the induction of seedling stem section, the induction of stem section can be promoted, after one month, add up survival rate, survival rate 89%.
Embodiment 2
Get sophora tonkinensis Gapnep stem with bud, surface contaminants removed by hairbrush, running water 3h, on superclean bench 70% ethanol postincubation 22s, aseptic water washing 3 times, the clorox process 13min of 0.2%, aseptic water washing 4 times, the sophora tonkinensis Gapnep stem with bud access HE+6-BA5mg/L+2 disinfected, the induction of axillalry bud is carried out in 4-D0.02mg/L medium, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 2200lx, light application time 13h/d, temperature 25 DEG C, the axillalry bud derived is put into medium HE+6-BA1.5mg/L+2ip0.5mg/L and is carried out squamous subculture, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 3000lx, light application time 15h/d, temperature 25 DEG C, bud seedling access medium 1/4MS+IBA0.7mg/L+GA after propagation
3root induction is carried out, additional saccharose 20g/L, agar 5.8g/L in 1mg/L, Ph6.0, illumination 12000lx, light application time 18h/d, temperature 25 DEG C, in units of every clump of 6 strains, cleans medium, 20s is dipped in the liquor potassic permanganate of 0.5%, be transplanted to fill with 0.5% disinfecting solution of potassium permanganate Nutrition Soil nutritive cube in, matrix is rural area soil: vermiculite=2:1, temperature 25 DEG C, relative air humidity 82%, sprays water 3 every day.Addition of gibberellin in the induction of seedling stem section, the induction of stem section can be promoted, after one month, add up survival rate, survival rate 87%.
Embodiment 3
Get sophora tonkinensis Gapnep stem with bud, surface contaminants removed by hairbrush, running water 3h, on superclean bench 70% ethanol postincubation 22s, aseptic water washing 3 times, the clorox process 13min of 0.2%, aseptic water washing 4 times, the sophora tonkinensis Gapnep stem with bud access HE+6-BA5mg/L+2 disinfected, the induction of axillalry bud is carried out in 4-D0.02mg/L medium, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 2200lx, light application time 13h/d, temperature 25 DEG C, the axillalry bud derived is put into medium HE+6-BA1.5mg/L+2ip0.5mg/L and is carried out squamous subculture, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 3000lx, light application time 15h/d, temperature 25 DEG C, bud seedling access medium 1/4MS+IBA0.7mg/L+GA after propagation
3root induction is carried out, additional saccharose 20g/L, agar 5.8g/L in 1mg/L, Ph6.0, illumination 12000lx, light application time 18h/d, temperature 25 DEG C, in units of every clump of 6 strains, cleans medium, 20s is dipped in the liquor potassic permanganate of 0.5%, be transplanted to fill with 0.5% disinfecting solution of potassium permanganate Nutrition Soil nutritive cube in, matrix is rural area soil: vermiculite=2:1, temperature 25 DEG C, relative air humidity 82%, sprays water 3 every day.Addition of gibberellin in the induction of seedling stem section, the induction of stem section can be promoted, after one month, add up survival rate, survival rate 93%.
Claims (4)
1. a method for quickly breeding for sophora tonkinensis Gapnep tissue cultures, comprise the acquisition of the aseptic bud of sophora tonkinensis Gapnep, the induction of bud, the propagation of Multiple Buds, root induction, acclimatization and transplants, its key step is as follows:
(1) sophora tonkinensis Gapnep stem with bud is got, to its disinfection;
(2) induction carrying out axillalry bud in the access of sophora tonkinensis Gapnep stem with bud HE+6-BA5mg/L+2, the 4-D0.02mg/L medium that step (1) disinfected is got, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 2200lx, light application time 13h/d, temperature 25 DEG C;
(3) get axillalry bud that step (2) derives to put into medium HE+6-BA1.5mg/L+2ip0.5mg/L and carry out squamous subculture, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 3000lx, light application time 15h/d, temperature 25 DEG C;
(4) the bud seedling access medium 1/4MS+IBA0.7mg/L+GA after step (3) propagation is got
3root induction is carried out, additional saccharose 20g/L, agar 5.8g/L, Ph6.0, illumination 12000lx, light application time 18h/d, temperature 25 DEG C in 1mg/L;
(5) get step (4) take root after test-tube plantlet carry out acclimatization and transplants.
2. according to the method for quickly breeding of a kind of sophora tonkinensis Gapnep tissue cultures according to claim 1, it is characterized in that: the acquisition of the aseptic stem with bud of sophora tonkinensis Gapnep described in step (1) is, get sophora tonkinensis Gapnep stem with bud, surface contaminants removed by hairbrush, running water 3h, on superclean bench 70% ethanol postincubation 22s, aseptic water washing 3 times, the clorox process 13min of 0.2%, aseptic water washing 4 times.
3. according to the method for quickly breeding of a kind of sophora tonkinensis Gapnep tissue cultures according to claim 1, it is characterized in that: in step (4), the method for sophora tonkinensis Gapnep acclimatization and transplants is in units of every clump of 6 strains, clean medium, 20s is dipped in the liquor potassic permanganate of 0.5%, be transplanted to fill with 0.5% disinfecting solution of potassium permanganate Nutrition Soil nutritive cube in, matrix is rural area soil: vermiculite=2:1, temperature 25 DEG C, relative air humidity 82%, sprays water 3 every day.
4. addition of gibberellin in the induction of seedling stem section, the induction of stem section can be promoted.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410540435.5A CN104221871A (en) | 2014-10-14 | 2014-10-14 | Fast reproduction method for Vietnamese sophora root tissue culture |
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| CN201410540435.5A CN104221871A (en) | 2014-10-14 | 2014-10-14 | Fast reproduction method for Vietnamese sophora root tissue culture |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105218238A (en) * | 2015-10-14 | 2016-01-06 | 广西壮族自治区药用植物园 | A kind of sophora tonkinensis Gapnep seedling medium and compound method thereof |
| CN105210854A (en) * | 2015-10-29 | 2016-01-06 | 山东省林业科学研究院 | A kind of method of Chinese scholar tree artificial pollination |
| CN106508675A (en) * | 2016-10-27 | 2017-03-22 | 潘克稳 | Seed tissue culture medium for root of subprostrate sophora |
| CN108184666A (en) * | 2017-12-29 | 2018-06-22 | 中国科学院华南植物园 | A kind of method for inspiring xylophyta bearing tree and sprouting the method for juvenile form bud and the quick breeding seedling based on juvenile form bud |
| CN113767851A (en) * | 2021-10-25 | 2021-12-10 | 山东省林业科学研究院 | Factory seedling raising method for new variety 'beautiful purple No. 1' of red flower Chinese scholartree |
-
2014
- 2014-10-14 CN CN201410540435.5A patent/CN104221871A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105218238A (en) * | 2015-10-14 | 2016-01-06 | 广西壮族自治区药用植物园 | A kind of sophora tonkinensis Gapnep seedling medium and compound method thereof |
| CN105210854A (en) * | 2015-10-29 | 2016-01-06 | 山东省林业科学研究院 | A kind of method of Chinese scholar tree artificial pollination |
| CN106508675A (en) * | 2016-10-27 | 2017-03-22 | 潘克稳 | Seed tissue culture medium for root of subprostrate sophora |
| CN108184666A (en) * | 2017-12-29 | 2018-06-22 | 中国科学院华南植物园 | A kind of method for inspiring xylophyta bearing tree and sprouting the method for juvenile form bud and the quick breeding seedling based on juvenile form bud |
| CN113767851A (en) * | 2021-10-25 | 2021-12-10 | 山东省林业科学研究院 | Factory seedling raising method for new variety 'beautiful purple No. 1' of red flower Chinese scholartree |
| CN113767851B (en) * | 2021-10-25 | 2024-04-16 | 山东省林业科学研究院 | Industrial seedling raising method for novel variety 'beautiful purple 1' of safflower Chinese scholartree |
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Application publication date: 20141224 |