CN104215475A - Filtering and sampling device for microorganisms at deep-sea hydrothermal vent - Google Patents
Filtering and sampling device for microorganisms at deep-sea hydrothermal vent Download PDFInfo
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- CN104215475A CN104215475A CN201410480715.1A CN201410480715A CN104215475A CN 104215475 A CN104215475 A CN 104215475A CN 201410480715 A CN201410480715 A CN 201410480715A CN 104215475 A CN104215475 A CN 104215475A
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Abstract
The invention discloses a filtering and sampling device for microorganisms at a deep-sea hydrothermal vent. The filtering and sampling device comprises a front-end collection tube, a suction pump, a plurality of filtering tanks and a controller. A water suction opening of the suction pump is fixedly connected with the front-end collection tube through a connecting hose, a water outlet of the suction pump is fixedly connected with all the filtering tanks through a multiway electromagnetic valve, a temperature sensor is fixedly arranged on the outer wall of the front-end collection tube, liquid outlets are formed in the tail ends of the filtering tanks, one-way valves for preventing external liquid from flowing back into the filtering tanks are fixedly arranged at the liquid outlets, a plurality of filtering membranes are sequentially fixedly arranged from inlets to the liquid outlets in the cavities of the filtering tanks, the filtering apertures of the filtering membranes are sequentially reduced, and the temperature sensor and the multiway electromagnetic valve are respectively electrically connected with the controller. The filtering and sampling device can be used for sampling a number of microorganisms at the hydrothermal vent during once submergence operation and is simple in structure, convenient to process and manufacture, low in cost and high in working efficiency.
Description
Technical field
The present invention relates to a kind of sampling apparatus, specifically, relate to a kind of deep-sea hydrothermal vent microbe filter sampling apparatus.
Background technology
Near the deep-sea hydrothermal vent of dark, poisonous, high temperature, high pressure, exist biocommunity in great numbers, the primary producer of this group is thermophilic bacteria and archeobacteria, its primary energy does not rely on photosynthesis, and derives from the chemical energy that Earth ejecting fluid provides.Hydrothermal vent is the window in research biosphere, deep, carries out hydrothermal vent thermophilic microorganism and learns correlative study, and for understanding, origin of life, biological one-tenth ore deposit etc. are significant.Carry out the correlative study of hydrothermal vent microbiology, directly depend on the quality of extracted microbiological specimens, fixed point in site measurement and the microbe filter sampling of carrying out hydrothermal vent fluid are crucial.Fixed point in site measurement object is to improve the purity of the microbiological specimens of sampling, and the purity of sample depends on the demarcation of the temperature to sampled hydrothermal solution, most important for the biocommunity under research specified temp.
Some current sampling apparatus structural principle complexity, as the publication number Chinese invention patent that is CN101034042, its sampling valve adopting is that single triggers drive principle, the nylon wire that need to fuse triggers sampling, reliability is low, if nylon wire cannot fuse in time or fusing too early, all cannot ensure that sampling task carries out smoothly, and can only carry out unitary sampling, recycling rate of waterused is low, because current sea floor sampling equipment is mainly sent into seabed by diving apparatus, carry out a sampling task and need to spend a large amount of manpower and materials costs, such sampling apparatus obviously can increase cost.
Summary of the invention
The present invention lacks purity demarcation measure and the low technical matters of reliability in order to solve existing deep-sea hydrothermal vent microbe filter sampling apparatus, and a kind of deep-sea hydrothermal vent microbe filter sampling apparatus is provided, and can address the above problem.
In order to solve the problems of the technologies described above, the present invention is achieved by the following technical solutions:
A kind of deep-sea hydrothermal vent microbe filter sampling apparatus, comprise front-end collection pipe, suction pump, several hay tanks, and controller, the water sucking mouth of described suction pump is fixedly connected with described front-end collection pipe by connecting hose, the water delivering orifice of described suction pump is fixedly connected with each hay tank respectively by various way solenoid valve, on described front-end collection pipe outer wall, be installed with temperature sensor, the end of described each hay tank has leakage fluid dram, described leakage fluid dram place is installed with for preventing the anti-retaining valve that enters hay tank of filling with of outside liquid, in the cavity of described hay tank, be installed with in turn some layers of filtering membrane by entrance to leakage fluid dram, the pore size filter of described each layer of filtering membrane reduces in turn, described temperature sensor and various way solenoid valve are electrically connected with described controller respectively.
Further, in order to filter the larger impurity such as the particle in gathered hydrothermal solution, the porch of described front-end collection pipe is installed with primary filter.
Further again, the porch of described hay tank is installed with secondary filter.
Preferably, in order to be convenient for changing the filtrator that uses different pore size, described primary filter modularization is pegged graft and is fixed on the porch of described front-end collection pipe.
Further, described several hay tanks are structure as a whole, and between adjacent hay tank, are separated by baffle plate.
In order to facilitate this device of manipulator clamping of manned underwater vehicle or remote underwater robot, on described front-end collection pipe outer wall, be installed with holding handle.
For convenient fixing and dismounting filtering membrane, in described hay tank, be installed with and described bed filtration film installation sheet one to one, described installation sheet is filter reticulate texture, described filtering membrane is separately fixed on corresponding installation sheet.
Further again, the water delivering orifice of described suction pump is threaded connection device and is connected with various way solenoid valve.
Wherein, the aperture of described each layer of filtering membrane is micron order.
Compared with prior art, advantage of the present invention and good effect are: deep-sea hydrothermal vent microbe filter sampling apparatus of the present invention, 1, the temperature sensor arranging by hydrothermal solution suction inlet place, can be used for demarcating sampling point position, realize fixed point microbe sampling, avoid institute's collected specimens to be polluted by the water sample of spout periphery, ensured the purity of collected specimens; 2, in each hay tank, have the microbe sampling filtering membrane of multilayer different pore size, dive operation once can realize a large amount of samplings of hydrothermal vent microorganism; 3, by the flexible replacing of different pore size filtrator and filtering membrane, can realize different sampling functions and effect; 4, simple in structure, be convenient to processing and manufacturing, with low cost, work efficiency is high.
Read by reference to the accompanying drawings after the detailed description of embodiment of the present invention, it is clearer that the other features and advantages of the invention will become.
Brief description of the drawings
In order to be illustrated more clearly in the embodiment of the present invention or technical scheme of the prior art, to the accompanying drawing of required use in embodiment or description of the Prior Art be briefly described below, apparently, accompanying drawing in the following describes is only some embodiments of the present invention, for those of ordinary skill in the art, do not paying under the prerequisite of creative work, can also obtain according to these accompanying drawings other accompanying drawing.
Fig. 1 is a kind of example structure schematic diagram of deep-sea hydrothermal vent microbe filter sampling apparatus proposed by the invention;
Fig. 2 is the cross section view of Fig. 1;
Fig. 3 is the inner structure schematic diagram of hay tank in Fig. 1;
Wherein, 101, front-end collection pipe; 102, temperature sensor; 103, primary filter; 104, clamping part; 201, suction pump; 301, hay tank; 302, retaining valve; 303, filtering membrane; 304, casing; 305, baffle plate; 306, installation sheet; 307, secondary filter; 401, connecting hose; 501, various way solenoid valve.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiment.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtaining under creative work prerequisite, belong to the scope of protection of the invention.
Embodiment mono-, the present embodiment provides a kind of deep-sea hydrothermal vent microbe filter sampling apparatus, as Fig. 1, shown in Fig. 2, comprise front-end collection pipe 101, suction pump 201, several hay tanks 301, and controller (not shown), the water sucking mouth of described suction pump 201 is fixedly connected with described front-end collection pipe 101 by connecting hose 401, the water delivering orifice of described suction pump 201 is fixedly connected with each hay tank 301 respectively by various way solenoid valve 501, on described front-end collection pipe 101 outer walls, be installed with temperature sensor 102, the end of described each hay tank 301 has leakage fluid dram, described leakage fluid dram place is installed with for preventing the anti-retaining valve 302 that enters hay tank of filling with of outside liquid, in the cavity of described hay tank 301, be installed with in turn some layers of filtering membrane 303 by entrance to leakage fluid dram, the pore size filter of described each layer of filtering membrane 303 reduces in turn, described temperature sensor 102 and various way solenoid valve 501 are electrically connected with described controller respectively.
The principle of work of the sampling apparatus of the present embodiment is: front-end collection pipe 101 is placed in to hydrothermal vent place, under the effect of suction pump 201, the hydrothermal solution that carries microorganism to be collected is drawn to various way solenoid valve 501 through front end collection tube, connecting hose 401, the guiding of controller switching various way solenoid valve 501, select and one of them hay tank 301 conducting, hydrothermal solution enters this hay tank 301 cavitys, after the filtration of each layer of filtering membrane 303, finally discharges through leakage fluid dram successively.Because the pore size filter of each layer of filtering membrane 303 reduces in turn, first the filtering membrane that larger-size microorganism is filtered tank 301 porch filters barrier firmly, the microorganism that size is less than this filtering membrane aperture continues to enter in hay tank 301 with hydrothermal solution, until by aperture be less than its size filtering membrane filter barrier live, the hydrothermal solution after filtration is discharged from.By filtering membrane aperture is reduced successively from hydrothermal fluid inflow direction, for filtering the microbiological specimens that gathers different size, having solved current sampling apparatus cannot point size sample, and the microorganism of various sizes is collected in a device, is unfavorable for analysis and research.And once sampling can gather different microorganisms respectively, because deep ocean work itself is just very difficult, need to drop into a large amount of manpower and materials, the convenient and swift Energy Efficiency Ratio that can greatly improve undoubtedly of this device.By be installed with temperature sensor 102 on front-end collection pipe 101 outer walls, in realizing the in site measurement of hydrothermal fluid temperature, can realize again the purity of gathered microbiological specimens and demarcate, realize high-purity sampling.The order of utilizing multiple directional control valve to realize many hay tanks 301 is switched, and can realize a large amount of samplings of thermophilic microorganism in katathermal solution fluid, has further improved deep ocean work efficiency.By adopting connecting hose 401 that suction pump 201 is fixedly connected with front-end collection pipe 101, realize in sampling process flexible mobile front-end collection pipe 101 under the prerequisite of moving filter tank 301 not, and then can gather diverse location hydrothermal solution mouth very easily.
Wherein, clamp described front-end collection pipe 101 and it is needed to displacement according to collection for convenient, preferably front-end collection pipe 101 is hard tube, in order to strengthen the intensity that is fixedly connected with of suction pump and various way solenoid valve, prevent from when suction pump 201 from working taking off pipe because Water Pressure causes, the water delivering orifice of described suction pump 201 is preferably connected with various way solenoid valve 501 by screw thread anchor ear.
Owing to may also having the solid particulate matters such as sandstone in hydrothermal solution; if being sucked to sampling by filtration device together, it may cause hay tank 301 to stop up; even destroy filtering membrane 303; in order to filter the larger impurity such as the particle in gathered hydrothermal solution; the porch of described front-end collection pipe 101 is installed with primary filter 103; larger impurity is filtered out by primary filter 103, can effectively avoid occurring hay tank 301 latch up phenomenons and protection each layer of filtering membrane 303 below.
The aperture of filtering membrane 303 can be selected to arrange according to actual needs flexibly, such as, selecting aperture is the filtering membrane in micron order aperture, can realize the collection of the microorganism to described micron order size.
The porch of described hay tank is installed with secondary filter 307, the solid particle polluter leaking for filtering primary filter.
According to the geographical environment situation of diverse location and the actual needs to institute's sampled targets, the pore size filter of primary filter 103 may need flexible adjustment, in order to be convenient for changing the filtrator that uses different pore size, described primary filter 103 is pegged graft and is fixed on the porch of described front-end collection pipe.As shown in Figure 1, such as offering with plug joint on the transverse section in front-end collection pipe 101 porch, primary filter 103 is inserted in this plug joint, dismounting is very convenient.
As a preferred embodiment, described several hay tanks 301 are integral type structure, and as shown in Figure 1, the overall surrounding of several hay tanks 301 is the casing 304 of sealing, and is separated by baffle plate 305 between adjacent hay tank 301.Owing to being provided with some layers of filtering membrane 303 in hay tank 301, and be distributed in the interior axial different depth position of hay tank 301 place, in order to facilitate the dismounting of each filtering membrane 303, the roof of casing 304 is for being detachably fixed on its sidewall.In addition, this hay tank 301 simple in structure, facilitates suitability for industrialized production.Casing 304 preferably adopts rectangular structure, in the time that this sampling by filtration device is carried or is placed on seabed by manned underwater vehicle or remote underwater robot, can fix than being easier to, while being placed on seabed, can prevent because the reasons such as terrain slope or hydrothermal solution impact are rolled.
In addition,, in order to facilitate this device of manipulator clamping of manned underwater vehicle or remote underwater robot, preferably on the outer wall of described front-end collection pipe 101, be installed with holding handle 104.
For convenient fixing filtering membrane 303, as shown in Figure 3, in described hay tank 301, be installed with and described bed filtration film 303 installation sheet 306 one to one, described installation sheet 306 is filter reticulate texture, described filtering membrane is separately fixed on corresponding installation sheet, can increase on the one hand the Area of bearing to filtering membrane 303, prevents that it is by hydrothermal solution impact failure, filter reticulate texture on the one hand in addition and heated fluid stream is not caused and stopped, ensure passing through smoothly of heated fluid stream.Wherein, installation sheet 306 can, by high strength glue bond on the inwall of hay tank 301, also can adopt other fixed forms such as clamping, clamping.
Certainly; above-mentioned explanation is not limitation of the present invention; the present invention is also not limited in above-mentioned giving an example, and variation, remodeling, interpolation or replacement that those skilled in the art make in essential scope of the present invention, also should belong to protection scope of the present invention.
Claims (9)
1. a deep-sea hydrothermal vent microbe filter sampling apparatus, it is characterized in that, comprise front-end collection pipe, suction pump, several hay tanks, and controller, the water sucking mouth of described suction pump is fixedly connected with described front-end collection pipe by connecting hose, the water delivering orifice of described suction pump is fixedly connected with each hay tank respectively by various way solenoid valve, on described front-end collection pipe outer wall, be installed with temperature sensor, the end of described each hay tank has leakage fluid dram, described leakage fluid dram place is installed with for preventing the anti-retaining valve that enters hay tank of filling with of outside liquid, in the cavity of described hay tank, be installed with in turn some layers of filtering membrane by entrance to leakage fluid dram, the pore size filter of described each layer of filtering membrane reduces in turn, described temperature sensor and various way solenoid valve are electrically connected with described controller respectively.
2. deep-sea hydrothermal vent microbe filter sampling apparatus according to claim 1, is characterized in that, the porch of described front-end collection pipe is installed with primary filter.
3. deep-sea hydrothermal vent microbe filter sampling apparatus according to claim 2, is characterized in that, the porch of described hay tank is installed with secondary filter.
4. deep-sea hydrothermal vent microbe filter sampling apparatus according to claim 2, is characterized in that, described primary filter modularization is pegged graft and is fixed on the porch of described front-end collection pipe.
5. deep-sea hydrothermal vent microbe filter sampling apparatus according to claim 1, is characterized in that, described several hay tanks are structure as a whole, and between adjacent hay tank, are separated by baffle plate.
6. according to the deep-sea hydrothermal vent microbe filter sampling apparatus described in claim 1-5 any one, it is characterized in that, on described front-end collection pipe outer wall, be installed with holding handle.
7. deep-sea hydrothermal vent microbe filter sampling apparatus according to claim 6, it is characterized in that, in described hay tank, be installed with and described bed filtration film installation sheet one to one, described installation sheet is filter reticulate texture, and described filtering membrane is separately fixed on corresponding installation sheet.
8. according to the deep-sea hydrothermal vent microbe filter sampling apparatus described in claim 1-5 any one, it is characterized in that, the water delivering orifice of described suction pump is threaded connection device and is connected with various way solenoid valve.
9. according to the deep-sea hydrothermal vent microbe filter sampling apparatus described in claim 1-5 any one, it is characterized in that, the aperture of described each layer of filtering membrane is micron order.
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| CN201410480715.1A CN104215475A (en) | 2014-09-19 | 2014-09-19 | Filtering and sampling device for microorganisms at deep-sea hydrothermal vent |
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| CN201410480715.1A CN104215475A (en) | 2014-09-19 | 2014-09-19 | Filtering and sampling device for microorganisms at deep-sea hydrothermal vent |
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Cited By (9)
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| CN105372095A (en) * | 2015-12-11 | 2016-03-02 | 国家深海基地管理中心 | Deep-sea organism suction-type multistage concentration sampling device |
| CN105432574A (en) * | 2015-11-23 | 2016-03-30 | 国家深海基地管理中心 | Carrying type deep sea macro-organism sampler |
| CN107132081A (en) * | 2017-07-14 | 2017-09-05 | 中国科学院海洋研究所 | Large Copacity original position Fluid sampling system |
| CN107449633A (en) * | 2017-08-18 | 2017-12-08 | 国家深海基地管理中心 | Erupt material capturing device in a kind of hot fluid area |
| CN108760046A (en) * | 2018-08-17 | 2018-11-06 | 上海大学 | A kind of luminous planktonic organism optically-captured device in deep-sea |
| CN110333095A (en) * | 2019-07-12 | 2019-10-15 | 杭州电子科技大学 | A kind of deep-sea suction-type plankton sampler |
| CN110541799A (en) * | 2019-08-19 | 2019-12-06 | 浙江大学 | Special constant delivery pump for deep-sea microorganism in-situ filtration |
| CN112504765A (en) * | 2020-10-20 | 2021-03-16 | 自然资源部第二海洋研究所 | Device and method for collecting submarine hydrothermal solution nozzle particles |
| WO2021139360A1 (en) * | 2020-08-06 | 2021-07-15 | 未名环境分子诊断（常熟）有限公司 | Sewage collection device for monitoring viruses and bacteria |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105432574A (en) * | 2015-11-23 | 2016-03-30 | 国家深海基地管理中心 | Carrying type deep sea macro-organism sampler |
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| CN107132081A (en) * | 2017-07-14 | 2017-09-05 | 中国科学院海洋研究所 | Large Copacity original position Fluid sampling system |
| CN107132081B (en) * | 2017-07-14 | 2023-06-13 | 中国科学院海洋研究所 | High-capacity in-situ fluid sampling system |
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| CN107449633B (en) * | 2017-08-18 | 2023-08-01 | 国家深海基地管理中心 | Hot liquid area eruption material trapping device |
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| CN110333095A (en) * | 2019-07-12 | 2019-10-15 | 杭州电子科技大学 | A kind of deep-sea suction-type plankton sampler |
| CN110541799A (en) * | 2019-08-19 | 2019-12-06 | 浙江大学 | Special constant delivery pump for deep-sea microorganism in-situ filtration |
| WO2021139360A1 (en) * | 2020-08-06 | 2021-07-15 | 未名环境分子诊断（常熟）有限公司 | Sewage collection device for monitoring viruses and bacteria |
| CN112504765A (en) * | 2020-10-20 | 2021-03-16 | 自然资源部第二海洋研究所 | Device and method for collecting submarine hydrothermal solution nozzle particles |
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