CN104208106A - Antrodia camphorate extract having human body immunity enhancing function, and its application - Google Patents
Antrodia camphorate extract having human body immunity enhancing function, and its application Download PDFInfo
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- CN104208106A CN104208106A CN201410400980.4A CN201410400980A CN104208106A CN 104208106 A CN104208106 A CN 104208106A CN 201410400980 A CN201410400980 A CN 201410400980A CN 104208106 A CN104208106 A CN 104208106A
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Abstract
The invention relates to an Antrodia camphorate extract having human body immunity enhancing function, and its application in the field of health foods, and belongs to the technical field of the health foods. The Antrodia camphorate extract is prepared through the following steps: fermenting Antrodia camphorate strain (ATCC200183) through a fermenting tank, centrifuging the obtained broth, abandoning the obtained supernatant, collecting the obtained precipitate, and freeze-drying at -30 - -50DEG C for 60-72h to obtain fermented Antrodia camphorate powder; and extracting the fermented Antrodia camphorate powder according to a solid/liquid mass ratio of 1:40-60 at 70-90DEG C by water 2-3 times, mixing the obtained water extraction liquids, filtering through a 200-300 mesh sieve to obtain a filtrate, carrying out reduced pressure concentration on the filtrate to a concentrate with the relative density of 1.05-1.15 (70-80DEG C), and freeze-drying the concentrate at -30 - -50DEG C for 60-72h to obtain the Antrodia camphorate extract. Experiments prove that the Antrodia camphorate water extract has a body immunity improving function, so the Antrodia camphorate water extract can be applied in the field of the health foods.
Description
Technical field
The invention belongs to technical field of health care food, be specifically related to a kind ofly there is the Antrodia Camphorata extract that strengthens immune function of human body and as the application in health food.
Background technology
The life style that Modern human populations is bad and custom: the motion of psychentonia, malnutrition, shortage or do not have enough sleep, abuse of alcohol and smoking etc., all can Immunosuppression system, reduce immunity of organisms.Other factors: the noxious substance in environment, some drugs, radiotherapy and excessive antibiotic also all can Immunosuppression systems.Along with growth in the living standard, take safety and health food effective people's a kind of means of enhancing immunity of taking like a shot especially.
Market is to the lifting of many medicinal fungi product consumptions, and traditional medicinal fungi training method can not meet the demand of market to medicinal fungi product, and liquid fermentation technology arises at the historic moment in the application of medicine trade.In recent years, medicinal fungi fermentation technique obtains applying more and more widely in improvement fermentation condition, raising product quality, reduction fermentation costs etc., is the research emphasis of current liquid fermentation.The present invention has investigated the medicinal fungi after liquid fermentation, and it is strengthening the effect in immunologic function etc., result shows, except aspects such as immune organ organ index, cellular immunization, humoral immunization, natural killer cells, this medicinal fungi is on the impact of the cytokine levels such as interleukin, interferon, they have on allogenic cell wide spectrum antiviral, affect Growth of Cells, and the multiple biological activity such as differentiation, immunity moderation function.For this reason, utilize macro fungi liquid submerged fermentation technology to prepare a kind of health food with significantly enhancing body immunity function to have great importance.
Antrodia camphorata, has another name called Antrodia Camphorata or Antrodia camphorata, belongs to Basidiomycetes, Aphyllophorales, porous section, perennial basidiomycetes, and only the inwall of rotten trunk is set in growth at the Cinnamomum kanahirai hay of Taiwan.Antrodia camphorata sporophore bitter in the mouth, has strong Lignum cinnamomi camphorae fragrance, and profile is tabular or mitriform, stockless.The sporophore initial stage presents Chinese red, Fructus Citri tangerinae brown time fresh, gradually can change sepia into, become black afterwards time aging.Find from research report in recent years, Antrodia Camphorata contains many complicated ingredients, as quintessence oil, polysaccharide body, its main chemical compositions is p-D-glucosan, three mushrooms compounds (as the acid of Camphor tree terpene A, B, e, K etc.), sudismase, nucleic acid materials such as () adenosines, ergosterol, protein (containing immune protein), vitamin, aminoacid, clusterin and some trace element etc.The most special with triterpenoid compound in these effective ingredient, up to more than 200 kinds, (Ganoderma of single strain is about 20-50 kind, total and the about kind more than 200 of different lines Ganoderma), content is 10%-45% (Ganoderma is 1%-3%), is that other mushroom bacterium cannot be compared; Polysaccharide body burden is 13.13% (Ganoderma is 2%).Based on polysaccharide, there is resisting fatigue, antitumor, raising immunity, blood sugar lowering, protect the liver, the multiple biological activity such as antioxidation, therefore the invention provides Antrodia Camphorata water extract improves Organism immunoregulation ability effect as raw material.
Summary of the invention
The object of this invention is to provide and a kind ofly there is the Antrodia Camphorata extract that strengthens immune function of human body and as the application in health food.
Of the present invention have the Antrodia Camphorata extract strengthening body immunity function, it prepares by the following method: by Antrodia Camphorata strain (ATCC200183) through ferment tank, fermentation liquid is centrifugal 5 ~ 10 minutes with 4000 ~ 5000 revs/min, abandon supernatant, collecting precipitation also with-30 ~-50 DEG C of lyophilizing 60 ~ 72 hours, obtains Antrodia Camphorata mycopowder; Again by Antrodia Camphorata mycopowder water extraction 2 ~ 3 times, solid-liquid mass ratio 1:40 ~ 60, temperature is 70 ~ 90 DEG C, and the time of water extraction first time is 3 ~ 4 hours, and the time of the 2nd water extraction is 1 ~ 3 hour, the time of the 3rd water extraction is 1 ~ 3 hour, merge repeatedly Aqueous extracts, 200 ~ 300 orders filter and obtain filtrate, filtrate are evaporated to relative density 1.05 ~ 1.15 (70 DEG C-80 DEG C), by concentrated solution with-30 ~-50 DEG C of lyophilizing 60 ~ 72 hours, obtain Antrodia Camphorata extract.
Antrodia Camphorata strain fermentation condition is: nutrient media components peptone 10 ~ 20g/L, sucrose 15 ~ 25g/L, magnesium sulfate 2 ~ 4g/L, potassium dihydrogen phosphate 2 ~ 4g/L, fermentation condition is: liquid amount is 60 ~ 80 liters, inoculum concentration (v/v) 2 ~ 5%, fermentation temperature 25 ~ 27 degrees Celsius, fermentation tank rotating speed 150 ~ 300 revs/min, fermentation time 3 ~ 5 days.
Prove (detailed in Example) through zoopery, Antrodia Camphorata water extract of the present invention has the function improving the anti-immunocompetence of body, thus can be applied as in health food.Its main Ergonomy effect is as follows: kunming mice took Antrodia Camphorata health food of the present invention (i.e. Antrodia Camphorata extract) after one month, its T lymhocyte transformation rate increases, NK cytoactive strengthens, delayed allergy strengthens, and meets the evaluation requirement to immunomodulating health food in " health food inspection and assessment technical specification " (2003 editions) completely.Especially, kunming mice took this product after one month, in serum, the level of the cytokine such as IL10, IL12, IL12R, IFN-α significantly improves, and the upper level-off of IFN-α is obviously better than each one-component independent role sum, and the rational proportion of compound recipe may produce synergism mechanism.
The obtained Antrodia Camphorata extract of present invention process safely, have no side effect, there is remarkable health-care effect, can long-term taking, and each primary raw material is produced by deep fermentation, reduces industrialization cost, have the good prospect of marketing.
Specific embodiment mode
Below in conjunction with embodiment, the invention will be further described, but the present invention does not limit by specific embodiment.
Embodiment 1
By Antrodia Camphorata strain (ATCC200183) through hectolitre ferment tank (nutrient media components: peptone 15g/L, sucrose 20g/L, magnesium sulfate 3g/L, potassium dihydrogen phosphate 3g/L, fermentation condition is: liquid amount is 70 liters, inoculum concentration (v/v) 3%, fermentation temperature 26 degrees Celsius, fermentation tank rotating speed 250 revs/min, fermentation time 4 days), fermentation liquid 68 liters is obtained after fermentation stops, centrifugal 10 minutes with 4000 revs/min, supernatant discarded, collecting precipitation also-40 DEG C of lyophilizing 66 hours, obtain Antrodia Camphorata mycopowder 924 grams, again mycopowder deionized water is carried 2 times, solid-liquid mass ratio is 1:40, temperature is 90 DEG C, 3 hours first times, 2nd time 1 hour, merge twice Aqueous extracts, with 200 order filter cloths, filtrate is obtained after filtration, filtrate being evaporated to relative density is 1.10 (80 DEG C), concentrated solution is obtained Antrodia Camphorata extract 108 grams in 72 hours with-45 DEG C of lyophilizing.
For verifying effect of the present invention further, test as follows:
1, animal grouping and raising
This test example is for illustration of Antrodia Camphorata health food of the present invention (the i.e. Antrodia Camphorata extract) impact on mouse immunity.Prepare healthy kunming mice 240, Quan Xiong, body weight 18 ~ 22g, raising temperature 22 ± 2 DEG C, relative humidity 40 ~ 70%, is divided into 4 large group at random, is respectively used to lymphocyte transformation experiment, the experiment of NK cytoactive detection, Tardive allergy and mouse spleen assessment of indices.Each large group 60 mices are divided into 3 groups more at random, every group 20, are respectively blank group, Antrodia Camphorata extract doses group and paecilomyces hepiall chen positive controls.Wherein extract doses group and positive control drug group press 300mg/kg dosage gastric infusion, and gavage volume is 0.2mL, and blank group is to the water of same volume; Every morning gavage 1 time, continuous 30 days.
2, testing result
2.1 this product are on the impact of Mouse Weight and index and spleen index
This product of table 1 is on the impact of Mouse Weight and index and spleen index
Each experimental mice body weight is compared with blank group, through statistical procedures, difference that there are no significant (P > 0.05), each experimental group index and spleen index is compared with blank group, through statistical procedures, difference that there are no significant (P > 0.05).Upper table result shows: the body weight change of this product to mice has no significant effect.The Main Immune Organs spleen weight of mice is had no significant effect.
2.2 this product are on the impact of mouse cell immunologic function
2.2.1 this product is on the impact of the mouse lymphocyte conversion test that ConA induces
Table 2 this product is on the impact of the mouse lymphocyte conversion test that ConA induces
Each experimental group group mice stimulation index SI, compared with blank group, through statistical procedures, all have significant difference, and extract doses group is compared with blank group, through statistical procedures, is all had significant difference, shows that this product can promote that mouse lymphocyte transforms.
2.2.2 this product is on the impact of mice delayed allergy
Table 3 this product is on the impact of mice delayed allergy
Each experimental mice swelling degree of the paw is compared with blank group, and through statistical procedures, each group has significant difference with blank group, shows that this product can promote that mice delayed is taken a stand and react.
Consolidated statement 2 and table 3 result, the test for celluar immunity result of this product to mice is positive.
2.3 this product are on the impact of NK cells in mice activity
Table 4 this product is on the impact of NK cells in mice activity
Each experimental mice NK cytoactive is compared with blank group, and through statistical procedures, each experimental group has significant difference, and extract doses group is slightly better than positive controls.
2.4 this product are on the impact of mice serum IFN-α cytokine levels
Table 5 this product is on the impact of mice serum IFN-α cytokine levels
Each cytokine levels in each experimental mice serum is compared with blank group, through statistical procedures, the IFN-α of each group has significant difference, raise IFN-alpha levels, and then strengthen natural killer cell (NK cell), macrophage and the lymphocytic vigor of T, thus play immunoregulation effect.
3 conclusions
Comprehensively above-mentioned, the continuous gavage of this product is after 30 days, the body weight of mice, spleen/body weight ratio are had no significant effect, aobvious positive to the test for celluar immunity result of mice, aobvious positive to the NK test cell line result of mice, and in general, the Antrodia Camphorata extract in the present invention, compared to positive controls, can play effect of enhancing human body immunity power better.Judge that this product has immunological enhancement according to Ministry of Health of the People's Republic of China " health food inspection and assessment technical specification " (version in 2003).
Embodiment 2
By Antrodia Camphorata strain (ATCC200183) through hectolitre ferment tank (nutrient media components: peptone 11g/L, sucrose 19g/L, magnesium sulfate 2.4g/L, potassium dihydrogen phosphate 2.7g/L, fermentation condition is: liquid amount is 65 liters, inoculum concentration (v/v) 2%, fermentation temperature 25 degrees Celsius, fermentation tank rotating speed 200 revs/min, fermentation time 3 days), fermentation liquid 62 liters is obtained after fermentation stops, centrifugal 10 minutes with 4000 revs/min, supernatant discarded, collecting precipitation also-40 DEG C of lyophilizing 66 hours, obtain Antrodia Camphorata mycopowder 814 grams, again mycopowder deionized water is carried 2 times, solid-liquid mass ratio is 1:50, temperature is 80 DEG C, 3 hours first times, 2nd time 1 hour, merge twice Aqueous extracts, with 200 order filter cloths, filtrate is obtained after filtration, filtrate being evaporated to relative density is 1.11 (80 DEG C), concentrated solution is obtained Antrodia Camphorata extract 92 grams in 65 hours with-50 DEG C of lyophilizing.
Embodiment 3
By Antrodia Camphorata strain (ATCC200183) through hectolitre ferment tank (nutrient media components: peptone 20g/L, sucrose 25g/L, magnesium sulfate 4g/L, potassium dihydrogen phosphate 3.7g/L, fermentation condition is: liquid amount is 78 liters, inoculum concentration (v/v) 5%, fermentation temperature 26 degrees Celsius, fermentation tank rotating speed 300 revs/min, fermentation time 5 days), fermentation liquid 75 liters is obtained after fermentation stops, centrifugal 10 minutes with 4000 revs/min, supernatant discarded, collecting precipitation also-40 DEG C of lyophilizing 66 hours, obtain Antrodia Camphorata mycopowder 1057 grams, again mycopowder deionized water is carried 2 times, solid-liquid mass ratio is 1:60, temperature is 70 DEG C, 3 hours first times, 2nd time 1 hour, merge twice Aqueous extracts, with 200 order filter cloths, filtrate is obtained after filtration, filtrate being evaporated to relative density is 1.07 (80 DEG C), concentrated solution is obtained Antrodia Camphorata extract 118 grams in 60 hours with-40 DEG C of lyophilizing.
Claims (3)
1. one kind has the Antrodia Camphorata extract strengthening immune function of human body, it is characterized in that: be prepare by the following method, by Antrodia Camphorata strain (ATCC200183) through ferment tank, fermentation liquid is centrifugal 5 ~ 10 minutes with 4000 ~ 5000 revs/min, abandon supernatant, collecting precipitation also with-30 ~-50 DEG C of lyophilizing 60 ~ 72 hours, obtains Antrodia Camphorata mycopowder; Again by Antrodia Camphorata mycopowder water extraction 2 ~ 3 times, solid-liquid mass ratio 1:40 ~ 60, temperature is 70 ~ 90 DEG C, and the time of water extraction first time is 3 ~ 4 hours, and the time of the 2nd water extraction is 1 ~ 3 hour, the time of the 3rd water extraction is 1 ~ 3 hour, merge repeatedly Aqueous extracts, 200 ~ 300 orders filter and obtain filtrate, relative density 1.05 ~ 1.15 at filtrate being evaporated to 70 DEG C-80 DEG C, by concentrated solution with-30 ~-50 DEG C of lyophilizing 60 ~ 72 hours, obtain Antrodia Camphorata extract.
2. a kind of as claimed in claim 1 have the Antrodia Camphorata extract strengthening immune function of human body, it is characterized in that: Antrodia Camphorata strain fermentation condition is: nutrient media components peptone 10 ~ 20g/L, sucrose 15 ~ 25g/L, magnesium sulfate 2 ~ 4g/L, potassium dihydrogen phosphate 2 ~ 4g/L, fermentation condition is: liquid amount is 60 ~ 80 liters, inoculum concentration (v/v) 2 ~ 5%, fermentation temperature 25 ~ 27 degrees Celsius, fermentation tank rotating speed 150 ~ 300 revs/min, fermentation time 3 ~ 5 days.
3. a kind of described in claim 1 or 2 has the Antrodia Camphorata extract that strengthens immune function of human body as the application in health food.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104940243A (en) * | 2015-06-02 | 2015-09-30 | 吉林大学 | Antrodia camphorate dropping pill with physical fatigue relieving function and preparation process |
| CN108329999A (en) * | 2018-02-09 | 2018-07-27 | 平潭迈康生物科技有限公司 | A kind of extracting method of Antrodia camphorata essential oil and its application in teenager's health care |
| CN110973452A (en) * | 2019-12-24 | 2020-04-10 | 刘先芝 | Health edible bird's nest food |
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| CN1799561A (en) * | 2005-09-28 | 2006-07-12 | 莱阳农学院 | Application of Antrodia camphorata mycelium fermented extract in preparation of anti-radiation damage medicine |
| CN103504285A (en) * | 2013-09-25 | 2014-01-15 | 江苏神华药业有限公司 | Method for extracting Antrodia camphorata mycelium based on membrane technology |
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2014
- 2014-08-14 CN CN201410400980.4A patent/CN104208106A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1799561A (en) * | 2005-09-28 | 2006-07-12 | 莱阳农学院 | Application of Antrodia camphorata mycelium fermented extract in preparation of anti-radiation damage medicine |
| CN103504285A (en) * | 2013-09-25 | 2014-01-15 | 江苏神华药业有限公司 | Method for extracting Antrodia camphorata mycelium based on membrane technology |
Non-Patent Citations (1)
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| 孙军德等: "樟芝液体发酵培养基配方的筛选优化", 《沈阳农业大学学报》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104940243A (en) * | 2015-06-02 | 2015-09-30 | 吉林大学 | Antrodia camphorate dropping pill with physical fatigue relieving function and preparation process |
| CN104940243B (en) * | 2015-06-02 | 2019-05-14 | 吉林大学 | A kind of Antrodia camphorata dripping pill and preparation process with function of physical fatigue alleviation |
| CN108329999A (en) * | 2018-02-09 | 2018-07-27 | 平潭迈康生物科技有限公司 | A kind of extracting method of Antrodia camphorata essential oil and its application in teenager's health care |
| CN110973452A (en) * | 2019-12-24 | 2020-04-10 | 刘先芝 | Health edible bird's nest food |
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Application publication date: 20141217 |