CN104206273A - Rapid propagation method for tissue culture of sparganium stoloniferum - Google Patents
Rapid propagation method for tissue culture of sparganium stoloniferum Download PDFInfo
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- CN104206273A CN104206273A CN201410463051.8A CN201410463051A CN104206273A CN 104206273 A CN104206273 A CN 104206273A CN 201410463051 A CN201410463051 A CN 201410463051A CN 104206273 A CN104206273 A CN 104206273A
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Abstract
The invention provides a rapid propagation method for tissue culture of sparganium stoloniferum. The rapid propagation method comprises the following steps: obtaining sparganium stoloniferum sterile buds, inducing the buds, propagating cluster buds, rooting outdoors, domesticating seedlings, transplanting and the like. The method establishes a tissue culture rapid propagation system of sparganium stoloniferum and provides certain material resources and a technological method for development and protection of wild resources of sparganium stoloniferum.
Description
Technical field
The present invention relates to the quick-breeding method of rhizoma scirpi tissue cultures, belong to field of plant growing technology.
Background technology
Rhizoma scirpi, Sparganiaceae, has another name called Rhizoma Sparganii, bubble Rhizoma Sparganii,
sparganium stoloniferum (Graebn.)buch.-Ham.ex Juz., perennial aquatic or swampweed basis, is usually born in the lake of height above sea level less than 1500 meters, stream, marsh, limit, pool shoal, only sees in 3600 meters of high mountain waters in China Tibet.Produce the provinces and regions such as Heilungkiang, Jilin, Liaoning, the Inner Mongol, Hebei, Shanxi, Shaanxi, Gansu, Xinjiang, Jiangsu, Jiangxi, Hubei, Yunnan.Also there is distribution in Afghanistan, Korea, Japan, Central Asia and Siberia and other areas, the Far East.The warm humid climate of kind happiness, should grow on the sunny side, in the environment of low humidity.Soil is required not tight, the shoal in irrigation canals and ditches, pond can be planted, also can be planted in paddy field.This kind of stem tuber is the conventional Chinese medicine of China, i.e. " Rhizoma Sparganii ", tool breaks the stasis of blood, promoting the circulation of qi, it is long-pending to disappear, pain relieving, stimulate the menstrual flow, the effect such as lactogenesis, be Important Economic plant in undergraduate course; Also for flower ornamental.Breed with stem tuber.The stem tuber that winter gathers in the crops, is put in visitor and preserves, and the stem tuber that the next spring stores or the stem tuber temporarily taken are propagating materials, and open cave, dark about 10cm by 30cm, every cave keeps flat stem tuber 2-3, waters clear water, often maintain water after cultivation.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of method for quickly breeding of rhizoma scirpi, and the method establishes the tissue-culturing rapid propagation system of rhizoma scirpi, for exploitation and protection rhizoma scirpi wild resource provide certain material source and technical method.
Technical problem to be solved by this invention is realized by following scheme:
Choose the young tender shoots of rhizoma scirpi stem tuber, running water 30min, on superclean bench 70% ethanol postincubation 30s, the mercuric chloride process 18min of 0.15%, aseptic water washing 5 times, soaks 30min, aseptic water washing 2 times with the sterile water of the cephalosporin containing 500mg/L, be placed in suck dry moisture on aseptic filter paper, the rhizoma scirpi bud grafting of disinfecting enters B
6the induction of bud is carried out, additional saccharose 30g/L, agar 6.5g/L in+6-BA2.5mg/L+IBA0.4mg/L+ZT0.8mg/L+500mg/L lactoalbumin hydrolysate medium, temperature 28 DEG C, intensity of illumination 2000lx, every day light application time 10h, PH5.8, humidity 85%, the bud derived puts into medium B
6+ 6-BA1.5-2.0mg/L+IBA0.3-0.4mg/L carries out the Multiplying culture of Multiple Buds, additional saccharose 50g/L, agar 6.5g/L, temperature 28 DEG C, intensity of illumination 5000lx, every day light application time 12h, PH5.8-6.0, humidity 85%, the bud seedling being about 3cm takes out, wash away base portion medium, morphology lower end 2-3cm is soaked in 10min in 1/2MS solution, plant in the rural area soil of sterilizing: vermiculite: in sawdust=3:1:1 matrix, use automatic sprinkler, within every three days, spray a 70-100ppmNAA solution, every carbendazim solution spraying 600 times for two weeks, root survival is added up after one month.
The rhizoma scirpi survival rate adopting the present invention to prepare is high, and the cycle is short, and output is large, pollutes little, is beneficial to implant mass.
Below in conjunction with embodiment, the present invention is further elaborated, but the scope of protection of present invention is not limited to following embodiments.
Embodiment
Embodiment 1
Choose the young tender shoots of rhizoma scirpi stem tuber, running water 30min, on superclean bench 70% ethanol postincubation 30s, the mercuric chloride process 18min of 0.15%, aseptic water washing 5 times, soaks 30min, aseptic water washing 2 times with the sterile water of the cephalosporin containing 500mg/L, be placed in suck dry moisture on aseptic filter paper, the rhizoma scirpi bud grafting of disinfecting enters B
6the induction of bud is carried out, additional saccharose 30g/L, agar 6.5g/L in+6-BA2.5mg/L+IBA0.4mg/L+ZT0.8mg/L+500mg/L lactoalbumin hydrolysate medium, temperature 28 DEG C, intensity of illumination 2000lx, every day light application time 10h, PH5.8, humidity 85%, the bud derived puts into medium B
6+ 6-BA1.5mg/L+IBA0.3mg/L carries out the Multiplying culture of Multiple Buds, additional saccharose 50g/L, agar 6.5g/L, temperature 28 DEG C, intensity of illumination 5000lx, every day light application time 12h, PH5.8-6.0, humidity 85%, the bud seedling being about 3cm takes out, wash away base portion medium, morphology lower end 2-3cm is soaked in 10min in 1/2MS solution, plant in the rural area soil of sterilizing: vermiculite: in sawdust=3:1:1 matrix, use automatic sprinkler, within every three days, spray a 70ppmNAA solution, every carbendazim solution spraying 600 times for two weeks, root survival is added up after one month, survival rate 88%.
Embodiment 2
Choose the young tender shoots of rhizoma scirpi stem tuber, running water 30min, on superclean bench 70% ethanol postincubation 30s, the mercuric chloride process 18min of 0.15%, aseptic water washing 5 times, soaks 30min, aseptic water washing 2 times with the sterile water of the cephalosporin containing 500mg/L, be placed in suck dry moisture on aseptic filter paper, the rhizoma scirpi bud grafting of disinfecting enters B
6the induction of bud is carried out, additional saccharose 30g/L, agar 6.5g/L in+6-BA2.5mg/L+IBA0.4mg/L+ZT0.8mg/L+500mg/L lactoalbumin hydrolysate medium, temperature 28 DEG C, intensity of illumination 2000lx, every day light application time 10h, PH5.8, humidity 85%, the bud derived puts into medium B
6+ 6-BA2.0mg/L+IBA0.4mg/L carries out the Multiplying culture of Multiple Buds, additional saccharose 50g/L, agar 6.5g/L, temperature 28 DEG C, intensity of illumination 5000lx, every day light application time 12h, PH5.8-6.0, humidity 85%, the bud seedling being about 3cm takes out, wash away base portion medium, morphology lower end 2-3cm is soaked in 10min in 1/2MS solution, plant in the rural area soil of sterilizing: vermiculite: in sawdust=3:1:1 matrix, use automatic sprinkler, within every three days, spray a 90ppmNAA solution, every carbendazim solution spraying 600 times for two weeks, root survival is added up after one month, survival rate 90%.
Embodiment 3
Choose the young tender shoots of rhizoma scirpi stem tuber, running water 30min, on superclean bench 70% ethanol postincubation 30s, the mercuric chloride process 18min of 0.15%, aseptic water washing 5 times, soaks 30min, aseptic water washing 2 times with the sterile water of the cephalosporin containing 500mg/L, be placed in suck dry moisture on aseptic filter paper, the rhizoma scirpi bud grafting of disinfecting enters B
6the induction of bud is carried out, additional saccharose 30g/L, agar 6.5g/L in+6-BA2.5mg/L+IBA0.4mg/L+ZT0.8mg/L+500mg/L lactoalbumin hydrolysate medium, temperature 28 DEG C, intensity of illumination 2000lx, every day light application time 10h, PH5.8, humidity 85%, the bud derived puts into medium B
6+ 6-BA2.0mg/L+IBA0.4mg/L carries out the Multiplying culture of Multiple Buds, additional saccharose 50g/L, agar 6.5g/L, temperature 28 DEG C, intensity of illumination 5000lx, every day light application time 12h, PH5.8-6.0, humidity 85%, the bud seedling being about 3cm takes out, wash away base portion medium, morphology lower end 2-3cm is soaked in 10min in 1/2MS solution, plant in the rural area soil of sterilizing: vermiculite: in sawdust=3:1:1 matrix, use automatic sprinkler, within every three days, spray a 100ppmNAA solution, every carbendazim solution spraying 600 times for two weeks, root survival is added up after one month, survival rate 91%.
Claims (3)
1. a method for quickly breeding for rhizoma scirpi tissue cultures, comprise the acquisition of the aseptic bud of rhizoma scirpi, the induction of bud, the propagation of Multiple Buds, outdoor rooting acclimatization and transplants, its key step is as follows:
(1) bud of rhizoma scirpi stem tuber is got, disinfection;
(2) get the rhizoma scirpi bud grafting that step (1) disinfected and enter B
6carry out the induction of bud in+6-BA2.5mg/L+IBA0.4mg/L+ZT0.8mg/L+500mg/L lactoalbumin hydrolysate medium, additional saccharose 30g/L, agar 6.5g/L, temperature 28 DEG C, intensity of illumination 2000lx, every day light application time 10h, PH5.8, humidity 85%;
(3) get the bud that step (2) derives and put into medium B
6+ 6-BA1.5-2.0mg/L+IBA0.3-0.4mg/L carries out the Multiplying culture of Multiple Buds, additional saccharose 50g/L, agar 6.5g/L, temperature 28 DEG C, intensity of illumination 5000lx, every day light application time 12h, PH5.8, humidity 85%;
(4) get step (3) cultivation Multiple Buds taking-up out and carry out outdoor rooting acclimatization and transplants.
2. according to the method for quickly breeding of a kind of rhizoma scirpi tissue cultures according to claim 1, it is characterized in that: the acquisition of the aseptic bud of rhizoma scirpi described in step (1) is, choose the young tender shoots of rhizoma scirpi stem tuber, running water 30min, on superclean bench 70% ethanol postincubation 30s, the mercuric chloride process 18min of 0.15%, aseptic water washing 5 times, soak 30min with the sterile water of the cephalosporin containing 500mg/L, aseptic water washing 2 times, is placed in suck dry moisture on aseptic filter paper.
3. according to the method for quickly breeding of a kind of rhizoma scirpi tissue cultures according to claim 1, it is characterized in that: in step (4), the hardening cultural method of taking root of goldenrod is that the bud seedling being about 3cm takes out, wash away base portion medium, morphology lower end 2-3cm is soaked in 10min in 1/2MS solution, plant in the rural area soil of sterilizing: vermiculite: in sawdust=3:1:1 matrix, use automatic sprinkler, within every three days, spray a 70-100ppmNAA solution, every carbendazim solution spraying 600 times for two weeks, added up root survival after one month.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109496853A (en) * | 2018-11-27 | 2019-03-22 | 钟天路 | A kind of construction method of trigone regenerating system |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109496853A (en) * | 2018-11-27 | 2019-03-22 | 钟天路 | A kind of construction method of trigone regenerating system |
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Application publication date: 20141217 |