CN104204523A

CN104204523A - Microfluidic pump

Info

Publication number: CN104204523A
Application number: CN201380014383.1A
Authority: CN
Inventors: 罗蒙斯·霍普特; 霍斯特·博尼克
Original assignee: Alere San Diego Inc
Current assignee: Alere San Diego Inc
Priority date: 2012-03-26
Filing date: 2013-03-15
Publication date: 2014-12-10
Also published as: EP2847465B1; EP2847465A4; US20150050172A1; EP2847465A1; WO2013148312A1; HK1208256A1

Abstract

A microfluidic pump is provided for managing fluid flow in disposable assay devices, which provides constant flows even at very low flow rates. Devices utilizing the microfluidic pump, as well as methods for manufacture and performing a microfluidic process are also provided.

Description

Micro-fluid pump

Background of invention

Field that the present invention belongs to

The present invention relates to micro-fluidic technologies, more particularly, relate to for controlling fluid by the micro-fluid pump of micro passage.

Background information

Microfluid system is to utilizing very small amount of fluid to obtain analytical chemistry and biological information is very effective.With microfluid system, can increase the response time, minimize sample size, reduce the consumption of reagent and article of consumption.When volatility or hazmat material are used or produce, in microfluid, carry out reaction and also improved Security and reduced and process quantity.

What microfluidic device became in various medical diagnostics and analytical chemistry and even genome and Proteomic analysis field becomes more and more important.They are also useful aspect treatment, for example, aspect low discharge administration.

For this purpose required micro-composition normally complicated and operating cost expensive.For example, micropump can be used to mix reagent and carry in system component arbitrarily between analysis platform and analytical instrument the fluid of the analyte tag reader of Presentation Function (as have).Yet in the scope of microfluidic device, control the mobile direction of fluid and speed, or in microfluidic channel, complete complex fluid flow pattern and be difficult to.

Summary of the invention

In single-time measurement device, for for reducing cost, improve the precision of the control of fluid sample, micro-fluid pump is studied.Device provided by the invention utilizes micro-fluid pump, and the method for manufacturing and carry out microfluid process.

Therefore, on the one hand, the invention provides micro-fluid pump module.In one embodiment, micro-fluid pump module comprises the first plate element and the second plate element, and the first plate has flexible, and the second plate does not have flexible.The second plate comprises a micro passage that forms in the second plate surface, and the first and second plates are linked together and along microfluid border, form Fluid Sealing, thereby are defined as fluid flow path.

On the other hand, the invention provides a kind of microfluidic device that utilizes micro-fluid pump module described here.Microfluidic device comprises that (a) surface has the rigid base of the microfluidic channel of shaping; Thereby the flexible layer sealing microfluidic channel that (b) covers rigid base's connection also, wherein flexible layer comprises a convex parts, this convex parts is arranged in part or all of micro passage.Device further comprises along edge, micro passage and is formed between rigid base and flexible layer and forms Fluid Sealing, forms the capillary tube of a sealing.

On the other hand, the invention provides a microfluidic device that utilizes micro-fluid pump module described here.This microfluidic device comprises that (a) surface has the rigid base of the microfluidic channel of shaping; Thereby (b) cover rigid base and the flexible layer sealing microfluidic channel of connection, wherein flexible layer comprises that one is arranged on the flat surface in part or all of micro passage.Device further comprises along edge, micro passage and is formed between rigid base and flexible layer and forms Fluid Sealing, forms the capillary tube of a sealing.

On the other hand, the invention provides a microfluidic device that utilizes micro-fluid pump module described here, wherein pump module comprises at least 2 independently micro passages that are arranged in parallel substantially.One or more breaks are used to be worked in two or more micro passages simultaneously, thereby provide certain methods that two kinds of mutual pumps of fluid are opened.Micro passage has identical cross-section area in certain embodiments, and like that, the fluid volume that each unit distance of micro passage is carried is essentially identical.In further embodiments, at least two micro passages have different cross-section areas, and in that example, the fluid volume of carrying each unit distance of micro passage is not identical.

On the other hand, the invention provides a microfluidic device that utilizes micro-fluid pump module described here, wherein pump module comprises at least two micro passages of independently arranging around the concentric circle of a point, puts at least one break can rotate according to this.In such embodiments, at least two micro passages have identical cross-section area, each rotation of at least one break, according to equation Q=r ω A, the fluid of more volume will be transferred in outmost passage, and Q is volume fraction, and r is the radius of micro passage, ω is angular velocity, and A is the cross-section area of micro passage.Therefore, if outmost passage has radius r 2, it is three times of inner channel radius r 1, thus break to rotate fluid volume that outermost passage carries will be three times of inner channel at every turn.Therefore, the people of the general technology of related domain will be easy to recognize, by changing coaxial micro passage cross-section area relative value separately, in each independent passage, each rotation can be carried the fluid of different volumes.

On the other hand, the invention provides for carrying out the method for microfluid process.The method comprises that (a) applies voltage on micro-fluid pump module as described herein.The voltage-activated motor applying, has accelerated brake component, for example one or more rotating shafts, and the rotatable joint of this rotating shaft the second basic unit, causes the distortion of the second basic unit, enters the micro passage forming in the first substrate surface.Thereby the second flexbile base enters micro-logical distortion road forces the fluid in micro passage to flow along micro-logical fluid that formed.The first basic unit be with Shao Er D hardness approximately 75 and approximately the material between 90 make.Such material includes, but not limited to polystyrene, polypropylene, polymethylmethacrylate, polycarbonate (PC) etc.The micro passage forming in the first substrate surface or the size of groove are stable, that means when the second basic unit distortion enters in the groove of the first basic unit, the width of groove be uncompressed state size at least about 75%, at least about 90%, at least about 95%, at least about 97.5%, at least about 99% or identical, and the height of groove be uncompressed state size at least about 75%, at least about 90%, at least about 95%, at least about 97.5%, at least about 99% or identical.Therefore as the distortion of the second basic unit, reach the result that enters the first basic unit, the boundary dimension of groove is considered to constant in fact.The second basic unit forms between approximately 15 and 90 such materials by having shore a hardness.Such material comprises but is not thermoplasticity flexible body (TPE), dimethyl silicone polymer (PDMS), silicone rubber, fluorine rubber etc.It is variable dimensionally that this material is considered to, and this refers to, when compressive force or tensile force are applied to such polymer material, and this material deformation, or be to extend toward one or more directions, or this material is toward one or more dimensions compressions.

On the other hand, the invention provides the method for manufacturing microfluidic device.The method comprises the rigid base that makes surface have micro passage, is connected with the flexible layer that covers rigid base and seal micro passage.Fluid is enclosed between the rigid base at the edge of micro passage and flexible layer and forms, and forms the capillary tube of a sealing.Rigid base and flexible layer are joined together by laser beam welding.This process comprises:

(a) in exposure rigid base or flexible layer, near ultraviolet laser energy edge, micro passage, makes the surperficial carbonization of rigid base or flexible layer.

(b) between rigid base and flexible layer, exert pressure;

(c) expose compressed rigid base and flexible layer under infrared energy, cause near local heating carburising surface in (a) and melt, so that sealing rigid base and flexible layer, thereby along the border of micro passage, form Fluid Sealing.

On the other hand, the invention provides the method for manufacturing microfluidic device.The method comprises the rigid base that makes surface have micro passage, is connected with the flexible layer that covers rigid base and seal micro passage.Fluid is enclosed between the rigid base at the edge of micro passage and flexible layer and forms, and forms the capillary tube of a sealing.The process that rigid base and flexible layer are manufactured by mould is joined together.This process comprises:

(a) the first component of polymer is expelled to an injection die cavities and forms rigid base;

(b) the second component of polymer is expelled to two injection die cavities and forms flexbile base; With

(c) allow the second polymer melted fuse and import to the first polymer material in (a), thus sealing rigidity and flexbile base, and the edge that is formed on passage forms Fluid Sealing.

The brief description of legend

Fig. 1 is a series of schematic diagram that in some embodiments of the invention, in microfluidic device operating process, various assemblies turn round.

Figure 1A is the viewgraph of cross-section of part microfluidic device in the embodiment of the present invention.

Figure 1B is the viewgraph of cross-section of part microfluidic device in the embodiment of the present invention.

Fig. 2 is the viewgraph of cross-section of part microfluidic device in the embodiment of the present invention.

Fig. 3 is the perspective view of part microfluidic device in the embodiment of the present invention.

Fig. 4 is the plan view of part microfluidic device in the embodiment of the present invention.

Fig. 5 is the perspective view of microfluidic device in the embodiment of the present invention.

Fig. 6 is a series of schematic diagram of microfluidic device in the embodiment of the present invention.

Fig. 6 A is the plan view of microfluidic device in the embodiment of the present invention.

Fig. 6 B is the plan view of microfluidic device in the embodiment of the present invention.

Fig. 6 C is the plan view of microfluidic device in the embodiment of the present invention.

Fig. 7 is the diagram that in the embodiment of the present invention, microfluidic device produces data.

Fig. 8 is the diagram that in the embodiment of the present invention, microfluidic device produces data.

Fig. 9 is the diagram that in the embodiment of the present invention, microfluidic device produces data.

Figure 10 is the plan view of part microfluidic device in the embodiment of the present invention.

Figure 11 is the cross sectional representation of the driver that uses in the embodiment of the present invention.

Figure 12 is the cross sectional representation of the driver that uses in the embodiment of the present invention.

Figure 13 is the schematic top plan view of microfluidic device in one embodiment of the present of invention.

Invention detailed content

In an embodiment, the disposable detection device that the micro-fluid pump that comprises pump and device have been developed for processing to portable example reduces low cost, the object of pinpoint accuracy and low discharge rate.Preferably, even when utmost point low discharge, the mobile speed of fluid in pump is that basic maintenance is constant.

Pump comprises that each other fixing the first basic unit and the second basic unit provide structure, has one or more micro passages in this structure, thereby this or these micro passage is along the sealed fluid flow that is defined as in border of micro passage.

About Figure 1A and 1B, one or more Micro Channel Architecture (40), as groove, form on the main surface of the first basic unit (20), as an inflexibility or rigid material.Deformable second basic unit (10) being formed, as the second basic unit of flexible body, interfixes to create airtight micro passage (40) with the first basic unit 20, and this micro passage has along the fluid-tight on the border of micro passage.Work as external force, for example, by for example rotating shaft of deformation element (50), be applied to flexible material (10), at least part of the second basic unit is compressed enter inflexibility composition (20) micro passage (40) thus in compression site obturation at least a portion micro passage (40).

Under compressive state, the second basic unit comes general inaccessible abundant micro passage (40) essence to replace the fluid from the major part compression site of micro passage (40).For example, the abundant part that the second basic unit may inaccessible this micro passage (40) is carried out separation of the fluid, being configured in the fluid-phase differentiation of compressing site opposite side in the fluid of compression site one side micro passage in and micro passage.In certain embodiments, under compressive state, the second basic unit's obturation, in compression site not compressed grooves cross-section area at least about 50%, at least about 75%, at least about 75%, at least about 90%, at least about 95%, at least about 97.5%, at least about 99%, or all areas.

Compression can created Fluid Sealing between the first and second basic units in the groove in compression site.When Fluid Sealing forms, fluid as liquid be prevented from from compression site a side flow to compression site opposite side.

Fluid Sealing can be temporary transient, as the second basic unit can loosen wholly or in part while removing pressure, thereby reopens groove wholly or in part.

Groove has first cross-section area under non-compressed state, and under compressive state, has second cross-section area.In certain embodiments, the part of flexible body is compressed enters groove and there is no and make groove distortion.The ratio that for example compresses the cross-section area in same site under the cross-section area in site and non-compressed state in compressive state is at least about 0.75, at least about 0.85, and at least about 0.925, at least about 0.975, or approximately 1.In certain embodiments, the height of groove, as the maximum height at compression site groove, under compressive state, can be under non-compressed state same site groove height at least about 75%, at least about 85%, at least about 90%, at least about 95%, at least about 100%.In certain embodiments, the width of groove, as the Extreme breadth in compressive state lower groove, under compressive state, can be under non-compressed state same site groove width at least about 75%, at least about 85%, at least about 90%, at least about 95%, at least about 100%.

The conversion of compression site (40) length along micro passage causes the effect of a pump, and the direction that this effect causes the fluid stream in micro passage (40) to advance towards deformation element (50) flows.In certain embodiments, a protruding component (30), a pump for example, be present on flexible material (10), this protruding component can be installed in part micro passage (40) above, thereby when compression inflexibility element (20), increase the thickness of flexible material to help flexible material closed channel.For example, under non-compressed state, flexible material can have the first thickness of covering groove, and is being positioned at the second thickness that has certain lateral separation with the center of groove.In certain embodiments, second Thickness Ratio the first thickness will be greatly at least about 110%, at least about 125%, at least about 150%, at least about 175%, at least about 200%.The second thickness can be than the first thickness greatly at least about 500% or still less, approximately 400% or still less, approximately 300% or still less, approximately 250% or still less.

The first distance can be at least about 1mm, at least about 2.5mm, at least about 5mm, at least about 1cm.The first distance can be about 2.5cm or still less, about 2cm or still less, about 1.5cm or still less, about 1.25cm or still less.In an embodiment, the first distance is larger at least about 1.5 times than width, at least about 1.75 times, at least about 2 times, at least about 4 times, as the Extreme breadth of groove.The first distance can be the width than groove, and the Extreme breadth of groove for example will be larger about 25 times or less, about 20 times or less, and about 15 times or less, about 10 times or less.

On the one hand, micro-fluid pump module (100) is used microfluidic structures as herein described.About 1A and 1B, microfluid module (100) comprises the first elastic basic unit element (10) and second rigid base's element (20) again.Second basic unit's element (20) is included in the micro passage (40) that is formed at second basic unit's element (20) surface, first and second basic unit's elements are joined together and the border of (40) forms Fluid Sealing along micro passage, thereby define fluid flowing path.

On the other hand, the microfluidic device (100) that utilizes micro-fluid pump module as herein described provided by the invention.Again about 1A and 1B, microfluidic device (100) is included in the rigid base (20) of the micro passage (40) of surface formation, the combination of Yu Gai rigid base, and cover rigid base (20) thereby the flexible layer (10) of sealing micro passage (40).

Another aspect, the invention provides a microfluidic device (100), and again about 1A and 1B, contrary mode is provided.In this example, micro passage (40) are formed on (10) in flexible layer; In rigid base (20), have a smooth surface profile, when flexible layer (10) can be attached thereto and cover rigid base (20) above, micro passage (40) are formed between this like that.

In various embodiments, flexible layer (10) comprises the protruding component (30) being arranged on suitable part or whole micro passage (40).Protruding component (30) has on the consistent region with micro passage (40) and has larger section thickness.This contributes to advance to enter between the micro passage (40) with microchannel surface at the elastic material (60) being out of shape to create water-stop.Those skilled in the art may understand to be that protruding component (30) can be a kind of in a lot of suitable shape, for example pump.In another embodiment, flexible layer (10) does not have protruding component (30), micro passage (40) are covered completely by flexible layer (10) in this case, and this flexible layer has smooth upper surface profile, this surface Bu Yu rigid base (20) contact.

By any appropriate technology well known in the art, one or more micro passages (40) can form on the surface of rigid base (20).For example micro passage can be by the deposition of mask material, chemical etching, laser lithography, moulding of plastic basic unit etc. and forming.Fluid Sealing also can be between rigid base (20) and flexible layer (10), and along micro passage, the edge of (40) forms the capillary tube of a sealing, thereby is defined as fluid flow.

For elastomer, with given speed, be progressively out of shape and enter micro passage, micro passage can define dimensionally the capacity of micro passage and generate flowing velocity.The high-precision micro passage of high quality shaping has like that formed micro-fluid pump element, and this element can be realized very slow and constant flow rate, if the manufacture process adopting is replaced, this may not can be implemented.Micro passage can be by designated size, and it has constant width and the constant degree of depth such length along some or all these micro passages.In one embodiment, along one section of micro passage of being combined with deformation element, micro passage will have a constant width and a constant degree of depth.Generally speaking, the width of micro passage is between 500 to 900 microns, and the degree of depth is between 40 to 100 microns.Device goes for the flow velocity of 0.001 microlitre/second to 5.0 microlitre/seconds in micro passage like that.

Can utilize the micro passage with various cross section figures.Figure 1A and 1B have described a micro passage (40), and the lower surface of this micro passage is arc, have defined the geometric figure of a recessed circle.Yet this can be understood, micro passage (40) can have circle, ellipse or common U-shaped bottom.In one embodiment, micro passage has curved bottom, and its radius of curvature is between 0.7 to 0.9 millimeter.Fig. 2 is the viewgraph of cross-section of part microfluidic device in embodiments of the present invention, and the concrete size of various features (with millimeter representing) is marked out above in the drawings.

The surface that it will be appreciated by those skilled in the art that micro passage (40) can be changed character, for example, by changing hydrophoby.For example hydrophoby can be modified by applying for example surface active agent of water wetted material, and the application of hydrophobic material, builds with the hydrophobic material of expectation, has the ionization surface of high energy beam, and/or analog.

As described herein, device of the present invention can comprise a plurality of micro passages (40), and each micro passage has multiple geometric figure and is configured in rigid base (20) upper (maybe can select on flexible layer) with various patterns.For example, micro passage (40) can be linear or along the arc expansion in surface of rigid base (20).Fig. 3 and 4 has shown as the micro passage of typical circular or helical configuration (40).Fig. 3 is the perspective view of device, and micro passage in this device (40) are configured to spirality, and more the micro passage of small volume is configured in the micro passage with larger volume.Fig. 4 is the plan view of device, and micro passage in this device (40) are configured and have port (100) and (110) in the mode of spiral, and these ports can circulate in fluid with one or more additional micro passages or structure.In one embodiment, the circle of micro passage or spiral section have the length of 20 to 100 millimeters.

By one with device radially or the deformation element being connected radially (50), the micro passage of helical or typical circular permission fluid advances through the micro passage of pump module or device.Fig. 5 is the detailed maps of pump module of the present invention and device, and in figure, a plurality of deformation elements (50) are connected with circular or spiral micro passage by (radially) radially.These deformation elements (50) are placed in housing (80), when structure contacts with deformation element (50) (spiral micro passage be configured in shown in the reverse side of sheet structure (110)), this housing is the one or more micro passages in microfluid sheet structure (110) of crosscut radially.Just as understood by a person skilled in the art, the sense of rotation of the deformation element (50) relevant with microfluid sheet structure (110) affects direction mobile in micro passage.Just because of this, those skilled in the art it will be understood that the fluid that flows through easily pump can be two-way.

By applying voltage to the motor of controlling its movement, housing (80) can be rotated.Just therefore so, the present invention further provides the method for implementing microfluid process, this process comprises that applying voltage arrives device as herein described.The voltage-activated that this applies motor, at least make a deformation element (50) advance, for example one or more rotating shafts, its can with the contacting of flexible the first basic unit's element (10) rotation, cause protruding component (30) distortion on flexible layer (10) to be inserted in the lip-deep micro passage of rigid base (20).

Various pulse per second (PPS)s can be applied to electric notor, thereby in micro passage, realize various flow velocity.Fluid flow is constant in essence, does not almost have shearing force to be applied on fluid, even under low-down flow condition.The feature of these pumps has improved the validity (if the integrity of analyte is to shear and sample that degraded is exposed is preserved by minimizing) of its performed analysis, and its low flow velocity provides time enough for the chemical reaction occurring.Low speed, the constant moving flow velocity of pump are also very useful aspect administration, guarantee the validity of batching.

In one embodiment, applying the flow velocity that pulse per second (PPS) result between 100 and 10000 produces by micro passage is 0.001 μ l/s to 5.0 μ l/s.Within the scope of very wide in range apply pulse, design of the present invention allows to be applied to suitable constant of the force retaining of micro passage of the present invention.

For example, Fig. 7-9th, the power producing in micro passage be take the plotted curve that pulses per second is function.In figure as Figure 7-9, the power producing in micro passage under very large pulse per second (PPS) scope is relatively constant, illustrates that substantially constant flow has minimum shearing force (shear).

Fig. 6 A-6C has shown structures different in different embodiments of the invention, in these embodiments, at least provides a spiral or circular micro passage.Circular or spiral micro passage (40) can be configured like that them can pass through port (100) and (110) are communicated with in fluid with one or more additional micro passages (140).Various reagent can be equipped in additional micro passage (140), is fixed on above micro passage, or provides in addition, and biologicall test can be implemented in fluid sample like that.

According to Fig. 1, as described herein, Fluid Sealing forms between rigid base (20) and flexible layer (10), and along micro passage, the capillary tube of a sealing of the edge of (40) formation is defined as fluid path.Figure 10 has shown the part of the device with common spirality micro passage, and in this micro passage, along micro passage, the edge of (40) is shown as Fluid Sealing (140).

Can utilize the whole bag of tricks Lai Shi rigid base (20) to be connected on the flexible material that forms flexible layer (10).These parts can adopt ultraviolet ray (UV) cure adhesive or other absorbent core but separated, and this makes to allow relatively moving of two parts before the rectification of tackiness agent/establishment key.Suitable tackiness agent comprises ultraviolet ray (UV) cure adhesive, thermosetting adhesive, and Pressuresensitive Adhesive, oxygen tackiness agent, and two-sided tape is bonding.

Selectable, this part can be utilized welding process coupling.Such process comprises ultrasonic bonding, hot weld connection and torsion welding process.

In further selection scheme, those parts can use post forming method (two-shot molding) or double-color mold (overmolding) connection, initial a kind of polymer under these circumstances, and then the second is injected in mould.Those skilled in the art can be easy to recognize that flexibility and inflexibility polymer can be connected the Fluid Sealing between these parts in such a way.

Method of laser welding is utilized in one embodiment.This process comprises:

(a) make one in first or second basic unit's element to be exposed under the ultraviolet luminous energy that is centered around edge, micro passage, so that the surface of carbonization first or second basic unit's element;

(b) between first and second basic unit's elements, exert pressure; With

(c) make compressed first and second basic unit's component exposure under infrared capable, cause near local heating the thawing of carburising surface of (a), in order to seal first and second basic unit's elements, thereby form Fluid Sealing along the border of micro passage.

The advantage of this method is that (i) this part in manufacture process can be controlled (mutually slide) to reach the direction of expectation, (ii) complicated shape can have in the geometric inflexibility-linearity of multiple passage or arcuate channel (or their combination) and be implemented at some, (iii) by non-flexible material, can realize and contacting of installing, non-flexible material is stable dimensionally.

In various embodiments, additional micro passage and structure can be provided biologicall test or the reaction that makes device can allow to implement number of different types.For example, additional fluid or reagent pond can be provided, and for example one or more fluids or reagent pond can be used as reaction chamber.Additional structure and shown in example, be below for illustrate the present invention rather than restriction the present invention.

In order to use diagnostic products cheaply to form instrument and article of consumption, subsequent embodiment has described the use of planar rondure of the present invention or spiral peristaltic pump, and due to potential pollution excessive risk, article of consumption need sealing there.

Two aspects are described, first aspect, with one very cheaply method come pump pressure fluid sample to storage dry chemical thing, this dry chemical thing is formulated in a position that is positioned at article of consumption inside, then fluid sample is mixed with the chemicals of storage.The second, with identical active pumping system diluted chemical thing, by diagnostic procedure, be also wherein a part for dilution.

These two aspects can be used together or separately.According to Figure 11-13, they will be illustrated respectively now.For the label used of the feature in Figure 11-13 of the present embodiment, for each figure, be all special, and can represent by another label in the application of Fig. 1-10.

According to Figure 11, pump pressure sample fluid is to the chemicals of depositing, the method that then sample fluid mixes with the chemicals of depositing, this method only relates to a break of use in mode cheaply, and for example merger is as DC in instrument or stepper motor (1).Peristaltic pump is comprised of micro passage (2) circle or that spiral ring has basic unit (3) characteristic of article of consumption, and the deformation film of this pump provides by flexible layer (5), by pump roller (6), allows flexible layer distortion.With annular pump coaxial be mixing chamber (7), this mixing chamber (7) comprises a ball magnetic force or magnetized (8).With instrument pump shaft be coaxially a structure that comprises mixing head (9), this mixing head (9) is that have magnetic force or magnetized and is connected on ball by magnetic.

From pump micro passage to mixing chamber, by the entrance and exit providing, pump is flowed and is connected with mixing chamber, and therefore when motor rotates, fluid can be pumped to mixing chamber from pump micro passage according to default direction.The instrument component of pump comprises that a suitable mechanism provides pump pressure and mixed function, when motor is during with a direction rotation, but when motor rotates in the opposite direction, only there is mixed function, for example by rotating shaft processed and Compress Spring, drive ratchet system, thereby mixing head and pump rotating shaft be with the preventive effect rotation of revolution, and when motor during with other direction rotations pump rotating shaft from motor, depart from, so only provide the rotation of mixing head.Compress Spring also can provide necessary contact force to promote effective pump pressure on pump channel.A series of processes are as shown in table 1 below.

table 1

Another embodiment provides pump channel inside or outside annular mixing chamber.Described in Figure 12, this embodiment can be created by the cost lower than first embodiment easily.Spiral or circular pump channel (1), is characterised in that basic unit (2) covers by elastic membrane (3) substantially, can be out of shape as described in Figure 11 in a similar fashion by pump rotating shaft (4).Yet in this special embodiment, mixing chamber is an annular pass coaxial with pump channel (4) but is positioned at the reverse side with pump channel basic unit.

What be positioned at this annular pass is one or a lot of bearing balls (5), these bearing balls (5) are magnetically coupled on the magnetic force or magnetization element on motor, like that when motor rotates, bearing ball also rotates in annular pass, has therefore caused the mixing that is stored at first the chemicals in annular pass.With the direction of the rotation of motor, realize and mixing and the driving mechanism of pump pressure, with when motor, take other directions only rotate as the mechanism of mixing be set to similar, as described in Figure 11.

According to Figure 13, the feature that has comprised the motor-driven system of describing in above-mentioned chapters and sections, described and utilized circle or spiral peristaltic pump in diagnostic assay process, to implement the method for dilution step: two coaxial circles or volute pump passage, be included in article of consumption, each has they self fluid passage, the fluid pump pressure of inner micro passage (1) sampling fluid (2) for example, outside micro passage (3) provide fluid pump pressure for diluted fluid (4).Identical pump motor (5) is shared in each micro passage, and the sample fluid that rotarily drives of such live axle by low cost motor is pumped together with buffer solution fluid.

More multithread body need to be pumped in different passages, and if necessary, this peristaltic pump is designed to hold the fluid passage of a plurality of different radiis.In this embodiment, first the sample that need to be transferred needs and is positioned at the chemicals (6) that mixing chamber (7) storage deposits and mixes, and then with diluted fluid, by dilution step, mixes.

Preferably, release fluid away from the chemicals of storage, the chemicals of storing like that can not be subject to the impact of diluted fluid.When motor is during with certain direction rotation, the pump rotating shaft being connected with pumping diaphragm carries sample fluid and diluted fluid to enter article of consumption, when mixing chamber is full of sample fluid, diluted fluid is full of the second Room (8), quantity and the geometrical shape of diluted fluid pump channel and the capacity of mixing chamber of the diluted fluid that the big or small set basis of this second Room is required.When motor stops, diluted fluid and sample fluid rest on their separately indoor.

If it is essential mixing, equivalent mechanisms, as mentioned above, can be implemented, and the motor of rotation only provides mixed function in the opposite direction.When sample fluid and diluted fluid must be united two into one, motor rotation carries sample and diluted fluid to a position of the article of consumption inside in conjunction with two kinds of fluids (9) in conjunction with pump rotating shaft.In order to help in conjunction with two kinds of fluids, passive mixed characteristic (10) is included in fluid calmodulin binding domain CaM.When motor continues to rotate two kinds of fluids of pumping, the sample being diluted can be transported to another position on article of consumption, for example, implement the position that analyte detects for one.

The present invention has some advantages.The first, due to design circle or function and the shape manufacture cost of spirality peristaltic pump be lowered.Because it is circular that pump designs some aspects or spiral geometric form makes only to use a break to become possibility; In this embodiment, it is electric motor, carries out different effects like that with the motor of a direction rotation from the motor of opposite direction rotation.For the article of consumption part of pump, the supplementary features of pump design are to have the ability to comprise a plurality of pump channels, and multiple fluid can be carried by same motor drive like that.

For example, if in a chemical reaction, amplified reaction is implemented, it may cause polluting, if or for the potential pollution of other reasons will be removed, pump design allows pump sealed from environment so.

Thereby various pulse per second (PPS) is applied on electric notor and in micro passage, realizes various flow velocity, comprises low-down flowing velocity.It is constant substantially that fluid flows, and does not almost have shearing force to be imposed on fluid, even when low-down flow rate.When low flow rate needs the abundant time for the chemical reaction that will occur, these features of pump have promoted the validity (if the integrity of analyte is that the sample sets that is exposed to shearing and degraded by minimizing assigns to preserve) of its analysis of carrying out.Low speed, constant pump flow rate also can be used to drug delivery, guarantees the validity of administration.

Although the present invention is described like this, also has otherwise variation under being appreciated that under invention marrow of the present invention and scope.Meanwhile, scope of the present invention is protected by following claim only.

Claims

1. a pump, comprising:

Passage, it is by the groove on first surface of (a) first basic unit, and (b) second surface of the second basic unit defines or forms; The break arranging is used for compressing in the groove that second surface enters the first basic unit, but does not make in fact groove distortion.

2. pump according to claim 1, wherein, described break is provided for moving along the axle of groove.

3. pump according to claim 1 and 2, wherein, described groove has height, and this is highly at least 10 microns, is at least 20 microns, is at least 30 microns, or is at least 50 microns.

4. according to the pump described in aforementioned any claim, wherein, described groove has height, this is highly about 1000 microns or still less, about 500 microns or still less, and about 250 microns or still less, about 125 microns or still less, about 100 microns or still less, about 75 microns or still less.

5. according to the pump described in aforementioned any claim, wherein, the first and second described basic units are in fact smooth.

6. according to the pump described in aforementioned any claim, wherein, described passage has outlet and entrance and the distance between entrance and exit, this distance is at least 1 millimeter, is at least 2.5 millimeters, is at least 5 millimeters, be at least 10 millimeters, be at least 25 millimeters.

7. according to the pump described in aforementioned any claim, wherein, described passage has outlet and entrance and the distance between entrance and outlet, this distance is at least 250 millimeters or still less, be at least 100 millimeters or still less, be at least 75 millimeters or still less, be at least 50 millimeters or still less, be at least 25 millimeters or still less.

8. according to the pump described in aforementioned any claim, wherein, described pump is configured to be communicated with microfluidic device fluid.

9. pump according to claim 8, wherein, described microfluidic device comprises that at least one microsome passage receives that suspection at least comprises the fluid sample of a target and micro passage comprises that at least one is used for determining the reagent whether described at least one target exists.

10. pump according to claim 9, wherein, described pump is set to, and when the liquid-vapor interface far-end of fluid sample is placed in the micro passage of this microsome device, at the liquid-vapor interface far-end of fluid sample, produces gas pressure effect.

11. pumps according to claim 10, wherein, the near-end of the liquid-vapor interface of described fluid sample is exposed in ambient air.

12. according to the pump described in claim 10-11, and wherein, the gas pressure effect producing at the liquid-vapor interface far-end of fluid sample is less than ambient atmosphere pressure.

Pump described in 13. according to Claim 8-12, wherein, the first and second basic units are placed in microfluidic device.

14. according to the pump described in claim 9-13, and wherein, the micro passage of microfluidic device comprises the fluid sample being positioned at wherein.

15. pumps according to claim 14, wherein, fluid sample comprises urine, or at least one fluid composition in blood.

16. according to the pump described in claim 9-15, wherein, described break is provided as fluid sample in the micro passage in microfluidic device and provides and be at least 1nl/s, is at least 5nl/s, is at least 10nl/s, be at least 25nl/s, be at least 50nl/s, be at least 100nl/s, be at least 250nl/s, be at least 500nl/s, or be at least the flow velocity of 1000nl/s.

17. according to the pump described in claim 9-16, wherein, described break is provided as fluid sample in the micro passage in microfluidic device and provides and be approximately 10,000nl/s or still less, be approximately 5,000nl/s or still less, is approximately 2500nl/s or still less, is approximately 1000nl/s or flow velocity still less.

18. according to the pump described in claim 9-17, and wherein, the total volume of the fluid sample in described micro passage is about 100 milliliters or still less, and about 50 milliliters or still less, about 25 milliliters or still less, about 20 milliliters or still less.

19. according to the pump described in aforementioned claim, wherein, passage has incompressible region, and when being braked device compression, compressed partially enclosed at least about 50% of this second basic unit, at least about 75%, at least about 90%, at least about 95%, at least about 97.5%, the not compressed region of at least about 99% described passage, or all not compressed region of essence closed channel.

20. according to the pump described in aforementioned claim, and wherein, at not compressed state, this groove has a width, at least about 50 microns of this width, at least about 100 microns, at least about 200 microns, at least about 500 microns.

21. pumps according to claim 20, wherein, under compressed state, the width of groove is at least about 75% of width under non-confined state, at least about 90%, at least about 95%, at least about 97.5%, at least about 99%, or essence is the same with non-compressed width.

22. according to the pump described in aforementioned claim, wherein, and at compressed state, this groove has a width, and this width is approximately 2000 microns or still less, about 1500 microns or still less, be approximately 1000 microns or still less, be approximately 750 microns, or be less than about 600 microns or still less.

23. pumps according to claim 22, wherein, under compressed state, the height of groove is at least about 75% of height under non-confined state, at least about 90%, at least about 95%, at least about 97.5%, at least about 99%, or essence is the same with non-compressed height.

24. according to the pump described in aforementioned claim, wherein, at not compressed state, cover the second basic unit on groove and have the first thickness and separate the second upper thickness of the first distance with channel lateral, and wherein, second described Thickness Ratio the first thickness will greatly at least about 110%, at least about 125%, at least about 150%, at least about 175%, or r at least about 200%.

25. pumps according to claim 24, wherein, described the first distance than the width of groove larger about 50%, the first described distance than the width of groove larger about 100%, the first described distance than the width of groove larger about 200%.

26. 1 methods, comprising:

To at least a portion of compressing the second surface of the second basic unit in the groove on the first surface of the first basic unit, but essence does not allow groove distortion, wherein, and at least a portion of the passage that this compression sealing is formed by second surface and the groove of the second basic unit.

27. methods according to claim 26, further comprise the different piece of compressing second surface, described different piece with along groove shaft to another skew.

28. according to the method described in claim 26 or 27, and wherein, described groove has a height, and this is highly at least about 10 microns, and at least about 20 microns, at least about 30 microns, at least about 50 microns.

29. according to the method one of claim 26-29 Suo Shu, and wherein, described groove has a height, this is highly about 1000 microns or still less, about 500 microns or still less, and about 250 microns or still less, about 125 microns or still less, about 100 microns or still less, about 75 microns or still less.

30. according to the method described in aforementioned any claim 26-29, and wherein, the first and second described basic units are substantial planar.

31. according to the method described in aforementioned any claim 26-30, and wherein, described passage has outlet and entrance and the distance between entrance and exit, this distance is at least 1 millimeter, is at least 2.5 millimeters, is at least 5 millimeters, be at least 10 millimeters, be at least 25 millimeters.

32. according to the method described in aforementioned any claim 26-31, wherein, described passage has outlet and entrance and the distance between entrance and exit, this distance is at least 250 millimeters or still less, be at least 100 millimeters or still less, be at least 75 millimeters or still less, be at least 50 millimeters or still less, be at least 25 millimeters or still less.

33. according to the method described in aforementioned any claim 26-32, wherein, further comprises to the micro passage in microfluidic device and introduces fluid sample, and this micro passage is that fluid is communicated with the passage fluid of the first and second basic units formation.

34. methods according to claim 33, wherein, described micro passage comprises that at least one is used for determining the reagent whether at least one target exists.

35. according to the method one of claim 33-34 Suo Shu, and wherein, the far-end of liquid-vapor interface of fluid sample that described being compressed in is placed in the micro passage of microfluidic device produces gas pressure effect.

36. methods according to claim 35, wherein, in described compression step, the near-end of the liquid-vapor interface of described fluid sample is exposed in ambient air.

37. according to the method described in claim 35 or 36, and wherein, the gas pressure effect producing at the liquid-vapor interface far-end of fluid sample is less than ambient atmosphere pressure.

38. according to the method one of claim 26-37 Suo Shu, wherein, the first and second basic units are placed in microfluidic device, and the method comprises a tag reader is placed in microfluidic device with exercisable relation, and this tag reader comprises the break that is configured to carry out compression step.

39. according to the method described in claim 38, wherein, further comprise to the micro passage of microfluidic device and introduce fluid sample, and operation tag reader is determined the existence of at least one target in microfluidic device.

40. according to the method described in claim 39, and wherein, fluid sample comprises urine, or at least one fluid composition in blood.

41. according to one of any described method of claim 26-40, wherein, described compression step is at least 1nl/s for the fluid sample in the micro passage in microfluidic device provides, and is at least 5nl/s, is at least 10nl/s, be at least 25nl/s, be at least 50nl/s, be at least 100nl/s, be at least 250nl/s, be at least 500nl/s, or be at least the flow velocity of 1000nl/s.

42. according to one of any described method of claim 26-41, wherein, described compression step is approximately 10 for the fluid sample in the micro passage in microfluidic device provides, 000nl/s or still less, be approximately 5,000nl/s or still less, is approximately 25000nl/s or still less at least, is approximately 1000nl/s or flow velocity at least still less.

43. according to one of any described method of claim 26-42, and wherein, the total volume of the fluid sample in described micro passage is about 100 milliliters or still less, and about 50 milliliters or still less, about 25 milliliters or still less, about 20 milliliters or still less.

44. according to one of any described method of claim 26-43, wherein, passage has incompressible region, and, in compressed step, compressed partially enclosed at least about 50%, at least about 75%, at least about 90% of this second basic unit, at least about 95%, at least about 97.5%, the not compressed region of at least about 99% described passage, or all not compressed region of essence closed channel.

45. according to one of any described method of claim 26-44, and wherein, at not compressed state, this groove has a width, at least about 50 microns of this width, at least about 100 microns, at least about 200 microns, at least about 500 microns.

46. according to one of any described method of claim 26-45, wherein, at compressed state, the width of groove is at least about 75% of width under non-confined state, at least about 90%, at least about 95%, at least about 97.5%, at least about 99%, or essence is the same with non-compressed width.

47. according to one of any described method of claim 26-46, wherein, at compressed state, this groove has a width, this width is approximately 2000 microns or still less, about 1500 microns or still less, is approximately 1000 microns or still less, be approximately 750 microns, or be less than about 600 microns or still less.

48. according to the method one of claim 47 Suo Shu, wherein, under compressed state, the height of groove is at least about 75% of height under non-confined state, at least about 90%, at least about 95%, at least about 97.5%, at least about 99%, or essence is the same with non-compressed height.

49. according to the method one of claim 48 Suo Shu, wherein, at not compressed state, cover the second basic unit on groove and have the first thickness and separate the second upper thickness of the first distance with channel lateral, and wherein, second described Thickness Ratio the first thickness will greatly at least about 110%, at least about 125%, at least about 150%, at least about 175%, or at least about 200%.

50. according to the method described in claim 49, wherein, described the first distance than the width of groove larger about 50%, the first described distance than the width of groove larger about 100%, the first described distance than the width of groove larger about 200%.

51. micro-fluid pump modules, comprise: the first and second basic units, wherein the first basic unit is comprised of the material of the Xiao A hardness between 25-70, and second basic unit by the material of the Shore D hardness between 80-90, formed, the second basic unit comprises in its surface at least one micro passage forming, thereby and wherein the first and second basic units be the formation tool capillary tube that links together.

52. according to the micro-fluid pump module described in claim 51, and wherein, at least one micro passage comprises that its partial-length is arc or straight line extends.

53. according to the micro-fluid pump module described in claim 52, and wherein, at least one micro passage comprises its partial-length, and this partial-length has formed general circle or spirality fluid path.

54. according to the micro-fluid pump module described in claim 52, and wherein, the length in described circle or spirality fluid path is 20-100 millimeter.

55. according to the micro-fluid pump module described in claim 51-54, and wherein, at least one micro passage has at least 10 millimeters, and 25 millimeters, 50 millimeters, the length dimension of 100 millimeters and 200 millimeters.

56. according to the micro-fluid pump module one of claim 51-55 Suo Shu, wherein, at least one micro passage has at least about 1000 microns on its all length or partial-length, at least about 900 microns, at least about, 800 microns, at least about 700 microns, at least about 600 microns, the width dimensions of at least about 500 microns and at least about 400 microns.

57. according to the micro-fluid pump module one of claim 51-56 Suo Shu, wherein, at least one micro passage has at least about 10 microns on its all length or partial-length, at least about 20 microns, at least about 30 microns, at least about 40 microns, at least about 50 microns, the depth dimensions of at least about 1500 microns.

58. according to the micro-fluid pump module described in aforementioned any claim, and wherein, at least one micro passage has arc, circular, the cross section of oval-shaped or U-shaped.

59. according to the micro-fluid pump module one of claim 51-58 Suo Shu, wherein, at least one micro passage has arc tangent plane, this tangent plane has at least about 0.5 millimeter, at least about 0.6 millimeter, at least about 0.7 millimeter, at least about 0.8 millimeter, at least about 0.9 millimeter, the radius of curvature of at least about 1 millimeter.

60. according to the micro-fluid pump module described in aforementioned any claim, and wherein, the rate of flow of fluid in micro passage can be controlled in the speed between 0.001 μ l/s-5.0 μ l/s.

61. according to the micro-fluid pump module described in aforementioned any claim, wherein, rate of flow of fluid in micro passage can be controlled in the speed of about 0.001 μ l/s, the speed of about 0.002 μ l/s, the speed of about 0.004 μ l/s, the speed of about 0.008 μ l/s, the speed of about 0.01 μ l/s, the speed of about 0.02 μ l/s, the speed of about 0.05 μ l/s, the speed of about 0.075 μ l/s, the speed of about 0.1 μ l/s, the speed of about 0.2 μ l/s, the speed of about 0.3 μ l/s, the speed of about 0.5 μ l/s, the speed of about 0.75 μ l/s, the speed of about 1 μ l/s, the speed of about 1.5 μ l/s, the speed of about 2 μ l/s, the speed of about 3 μ l/s, the speed of about 4 μ l/s, the speed of about 5.0 μ l/s.

62. according to the micro-fluid pump module one of claim 51-61 Suo Shu, and wherein, the first plate element comprises the thickness that section increases, and this thickness is consistent with the micro passage on the second plate element.

63. according to the micro-fluid pump module described in claim 62, and wherein, the thickness that section increases is set to the pump or the spine that on the first plate element, form, and wherein pump has the width of 700-1400 micron and the height of 40-150 micron.

64. according to the micro-fluid pump module described in claim 63, and wherein, the protruding surface of pump has the radius of curvature of 0.7-1.0 millimeter.

65. according to the micro-fluid pump module described in claim 64, and wherein, the thickness that section increases extends along the length direction of all or part micro passage.

66. according to the micro-fluid pump module described in claim 65, and wherein, the hatch region of the thickness that section increases is greater than the hatch region of the micro passage meeting with it.

67. according to the micro-fluid pump module described in claim 51, wherein, further comprise the rotatable deformation element being positioned on the first plate element, this deformation element is set to allow the part of the first plate element be out of shape in the micro passage on the second surface of the second plate element.

68. according to the micro-fluid pump module described in claim 61, wherein, further comprise the rotatable deformation element being positioned on the first plate element, this deformation element is set to allow the projection thickness of the first plate element be out of shape in the micro passage on the second surface of the second plate element.

69. according to the micro-fluid pump module described in claim 68, wherein, this deformation element is set to allow the part of the first plate element, or projection thickness being out of shape in micro passage along the length of micro passage with increasing, thereby propulsive fluid moves along the length of micro passage.

70. according to the micro-fluid pump module described in claim 67, and wherein, this deformation element is the rotatable cylindrical roller with outer surface being positioned on the first plate element, and wherein, the hardness of this cylindrical roller is greater than the first plate element.

71. according to the micro-fluid pump module described in claim 70, wherein, further comprises rotatable at least 2 cylindrical roller that are positioned on the first plate element.

72. according to the micro-fluid pump module described in claim 51, wherein, further comprises the other micro passage that at least one forms on the surface of the second plate.

73. according to the micro-fluid pump module described in claim 72, wherein, at least two micro passages with one of them concentric, with ring or with the form of spiral by adjacent setting.

74. according to the micro-fluid pump module described in claim 73, and wherein, each micro passage has different tangent plane regions.

75. according to the micro-fluid pump module described in claim 71, and wherein, the first and second plate elements have the thickness of 40-100 micron.

76. according to the micro-fluid pump module one of claim 51-71 Suo Shu, and wherein, the first and second plate elements are joined together by tackiness agent.

77. according to the micro-fluid pump module described in claim 76, and wherein, tackiness agent comprises medical UV tackiness agent, heat bonding reagent, pressure sensitive adhesive, oxidation-sensitive tackiness agent and double-sided belt tackiness agent.

78. according to the micro-fluid pump module one of claim 51-71 Suo Shu, and wherein, the first and second plate elements are joined together by the mode of welding.

79. according to the micro-fluid pump module described in claim 78, and wherein, the mode of welding comprises ultra-sonic welded, laser bonding, thermal weld and torsion welding.

80. according to the micro-fluid pump module one of claim 51-79 Suo Shu, and wherein, first and second basic unit's elements are joined together by the mode of laser bonding, and described welding process comprises:

(a) be that first or second basic unit's component exposure is under near uv energy micro passage, so that the surface of carbonization first or second basic unit's element;

(b) between first and second basic unit's elements, exert pressure; With

81. microsome devices, comprising:

(a) rigid base, has micro passage in its surface; With

(b) be connected and cover the flexible layer of rigid layer with rigid layer, thereby described micro passage is sealed, wherein, described flexible layer is included in the protruding member on part or all of micro passage.

82. microfluidic devices described in 1 according to Claim 8, are further included in the liquid sealing between the peripheral Shang rigid base in micro passage and flexible layer, thereby form the capillary tube of sealing.

83. microfluidic devices described in 2 according to Claim 8, the element of its protrusions is pump.

84. microfluidic devices described in 3 according to Claim 8, wherein, pump has the width of 700-1400 micron and the height of 40-150 micron.

85. microfluidic devices described in 4 according to Claim 8, wherein, the surface of the projection of pump has the radius of curvature of 0.7-1.0 millimeter.

86. microfluidic devices described in 1 according to Claim 8, wherein, micro passage has the width dimensions of 500-900 micron and the depth dimensions of 40-100 micron.

87. microfluidic devices described in 1 according to Claim 8, wherein, micro passage has arc, circular, the cross section end of oval-shaped or U-shaped.

88. microfluidic devices described in 7 according to Claim 8, wherein, micro passage has arc end, and this end has the radius of curvature of 0.7-0.9 millimeter.

89. microfluidic devices described in 2 according to Claim 8, wherein, described capillary tube is set to the flow velocity of the 0.001-5.0 μ l/s in capillary tube.

90. microfluidic devices described in 1 according to Claim 8, wherein, micro passage comprises the partial-length extending with arc.

91. according to the microfluidic device described in claim 90, and wherein, described arcuate section has formed general circular or spiral fluid path.

92. according to the microfluidic device described in claim 91, and wherein, described arcuate section has the length of 20-100 millimeter.

93. according to the microfluidic device described in claim 90, and wherein, micro passage further comprises the partial-length extending linearly.

94. according to the microfluidic device described in claim 90, and wherein, linear segment has the length dimension of 0.5-10 centimetre.

95. according to the microfluidic device described in claim 93, and wherein, micro passage comprises at least two partial-lengths to extend linearly.

96. microfluidic devices described in 1 according to Claim 8, wherein, further comprise other, the micro passage forming on the surface of rigid base.

97. according to the microfluidic device described in claim 96, wherein, the neighbour setting mutually on the surface of rigid base of at least two micro passages with arcuate section, arcuate section is according to one heart, with ring or with the mode of spiral, arranges.

98. according to the microfluidic device described in claim 97, and wherein, each micro passage has different cross-section.

99. microfluidic devices described in 1 according to Claim 8, wherein, each rigidity and flexible basic unit have the thickness of 40-100 micron.

100. microfluidic devices described in 1 according to Claim 8, wherein, rigidity and flexible link base layer are crossed tackiness agent and are joined together.

101. according to the microfluidic device described in claim 100, and wherein, tackiness agent comprises medical UV tackiness agent, heat bonding reagent, pressure sensitive adhesive, oxidation-sensitive tackiness agent and double-sided belt tackiness agent.

102. according to the microfluidic device described in claim 100, and wherein, the mode that rigidity and flexible link base layer are crossed welding is joined together.

103. according to the microfluidic device described in claim 102, and wherein, the mode of welding comprises ultra-sonic welded, laser bonding, thermal weld and torsion welding.

104. according to claim 103, and wherein rigid base and flexible layer couple together by laser bonding, and this process comprises:

(a) rigid base and flexible layer are exposed under near ultraviolet energy micro passage, so that the surperficial carbonization of rigid base or flexible layer;

(b) between rigid base and flexible layer, exert pressure; With

(c) rigid base and the flexible layer of compression are exposed in infrared energy, cause near the local heating of carburising surface in (a) and melt, so that sealing rigid base and flexible layer, thereby the border formation Fluid Sealing of checking micro passage.

105. one kinds of methods of carrying out microfluid process, comprising:

(a) to micro-fluid pump module, apply voltage, described micro-fluid pump module comprises:

(i) the first plank element being connected with the second plank element; Wherein, the first plank element is flexible, and the second plank element is stiff, and the second plank element is included in the micro passage that the second plank element surface forms; With, the first plank element is included in the protruding component arranging on part or all of micro passage;

Wherein, further, the first and second plank elements are connected to form liquid sealing on the border along limiting the micro passage of microfluidic pathways;

(ii) be rotatably positioned at the Crumple element of the first plank element, the voltage of this deformation element based on applying is set to move along the length direction of micro passage;

(b) allow Crumple element move along the length direction of micro passage; Wherein, the movement of Crumple element causes protruding member to micro passage, to carry out deformation, thereby forces the fluid that is positioned at micro passage to flow along fluid path.

106. according to the method described in claim 105, and wherein, described Crumple element is cylindrical roller, and it has the outer surface that is rotatably connected to protruding member, and wherein, the hardness of cylindrical roller is greater than the element of projection.

107. according to the method described in claim 106, and wherein, micro-fluid pump further comprises at least two cylindrical rollers, and these at least two cylindrical rollers are arranged on the one or more protruding members that are positioned on micro passage rotationally.

108. according to the method described in claim 105, and wherein, micro-fluid pump is applicable to the flow velocity of the 0.001-5.0 μ l/s in micro passage.

109. according to the method described in claim 105, wherein, thereby further comprises that the direction of reverse Crumple element makes the direction of the fluid in micro passage reverse.

110. according to the method described in claim 105, and wherein, micro passage comprises that partial-length is arc extension.

111. according to the method described in claim 110, and wherein, micro passage comprises limits common circle or the partial-length of spirality stream.

112. according to the method described in claim 105, and wherein, micro-fluid pump is further included at least other micro passage forming on the second plank element surface.

113. according to the method described in claim 112, wherein, at least two micro passages on the surface of rigid base according to one heart, with ring or with the mode of spiral neighbour setting mutually.

114. according to the method described in claim 113, and wherein, each micro passage has different cross-section.

115. according to the method described in claim 105, wherein, further comprises and detecting by the analyte in fluid or biological respinse.

116. one kinds of methods of manufacturing microfluidic device comprise: the rigid base on surface with micro passage is connected with the flexible layer that covers rigid base and seal micro passage, periphery along micro passage, liquid sealing is formed between rigid base and flexbile base, thereby form the capillary tube of sealing, wherein, the mode that rigidity and flexible link base layer are crossed laser bonding is joined together, and which comprises:

(b) between rigid base and flexible layer, exert pressure; With

117. according to the pump one of claim 1-25 Suo Shu, and the mode that wherein the first and second link base layers are crossed laser bonding is joined together.

118. one kinds of methods of producing the described pump of one of claim 1-26, comprise, by containing the mode of using from irradiation part first and second basic units of laser, the first and second pumps are linked together.

119. according to the pump one of claim 1-25 Suo Shu, and wherein, under non-compressed state, the second surface of the second basic unit is substantial planar.

120. according to the method one of claim 26-50 Suo Shu, and wherein, under non-compressed state, the second surface of the second basic unit is substantial planar.

121. according to the pump one of claim 1-25 Suo Shu, and wherein, groove caves in, leg-of-mutton, square or rectangle.

122. according to the pump one of claim 1-25 Suo Shu, and wherein, groove has major axis, and break is set to move along the major axis of groove.

123. according to the pump described in claim 122, and wherein, pump is set to move a certain distance along the major axis of groove, thereby can be taken to few 500nl by pump, at least 1 microlitre, at least 2.5 microlitres, at least 5 microlitres, at least 10 microlitres, the volume of at least 205 microlitres.

124. according to the pump described in claim 122, and wherein, pump has pump and is taken to few 10ml or total volume still less, at least 5ml or total volume still less, at least 1ml or total volume still less, at least 0.5ml or total volume still less.

125. according to claim 1-25, or the pump that one of 121-124 is described, wherein, described break is set to compression member the second basic unit in groove, simultaneously or subsequently, along at least 1 millimeter, at least 2.51 millimeters, at least 5 millimeters, at least 10 millimeters, at least 20 millimeters, or the distance of at least 50 millimeters is compressed.

126. according to claim 1-25, or the described pump of one of 121-125, wherein, described break is set to compression member the second basic unit in groove, simultaneously or subsequently, and along at least 100 millimeters or still less, at least 75 millimeters or still less, at least 50 millimeters or Range compress still less.

127. according to claim 1-25, or the described pump of one of 121-126, and wherein, described break is set to move along the major axis of groove the position of compression.

128. according to claim 1-25, or the described pump of one of 121-127, and wherein, described break is set to, in the given time, separately to the second basic unit of compression member in groove.

129. according to the pump described in claim 128, and wherein, break is set to move along at least part of groove the second basic unit of single compressed part, is selected from 25% of slot length, 35% of slot length, 50% of slot length, 75% of slot length.

130. according to claim 1-25, or the pump that one of 121-129 is described, wherein, in any time, being compressed to the second basic unit in groove partly has and is less than about 50% of whole slot length, be less than the about 30% of whole slot length, be less than the about 25% of whole slot length, be less than about 20% of whole slot length, be less than the about 15% of whole slot length, be less than about 10% of whole slot length.

131. according to claim 26-50, and one of 120 described methods, and wherein, groove caves in, leg-of-mutton, square or rectangle.

132. according to claim 26-50, and one of 120 and 131 described methods, and wherein, groove has major axis, and the step of compression comprises along the major axis of at least part of groove and moves break.

133. according to claim 26-50, and 120 and the described method of one of 131-132, wherein, the step of compression comprises simultaneously or the sufficiently long groove of compression subsequently comes pump to be taken to few 500nl, at least 1 microlitre, at least 2.5 microlitres, at least 5 microlitres, at least 10 microlitres, the volume of at least 205 microlitres.

134. according to claim 26-50, with 120 and the described method of one of 131-133, wherein, the step of compression comprises simultaneously or the sufficiently long groove of compression subsequently comes pump to be taken to few 10ml or total volume still less, at least 5ml or total volume still less, at least 1ml or total volume still less, at least 0.5ml or total volume still less.

135. according to claim 26-50, and 120 and the described method of one of 131-134, wherein, the step of compression comprise simultaneously or subsequently along at least 1 millimeter of the distance of groove, at least 2.51 millimeters, at least 5 millimeters, at least 10 millimeters, at least 20 millimeters, or at least 50 millimeters compressed.

136. according to claim 1-25, and the pump described in 121-125, and wherein, break is set to be approximately 100 millimeters or still less along distance, and at least 75 millimeters or still less, at least 50 millimeters or still less carry out compression groove.

137. according to claim 1-25, and the pump described in 121-126, and wherein, break is set to implement fixed point compression along the major axis of groove.

138. according to claim 1-25, and the pump described in 121-127, and wherein, break is set to, in any given time, only to the unitary part of compressing the second basic unit in groove.

139. according to the pump described in claim 128, and wherein, break is set to move along at least part of groove the second basic unit of single compressed part, is selected from 25% of slot length, 35% of slot length, 50% of slot length, 75% of slot length.

140. according to claim 1-25, or the pump that one of 121-129 is described, wherein, along groove, to the part of the second basic unit compressing in groove, there is the about 50% of at least whole slot length, be less than about 30% of whole slot length, be less than about 25% of whole slot length, be less than the about 20% of whole slot length, be less than the about 15% of whole slot length, be less than about 10% of whole slot length.

141. according to the pump of aforementioned any claim, and wherein two or more grooves are provided in the first basic unit, thereby forms at least two discontinuous passages.

142. according to the pump described in claim 141, and wherein, at least two discontinuous passages have width w1 and width w2, wherein width w1 is at least 75% of width w2,90% of width w2,95% of width w2,97.5% of width w2,99% of width w2, or essence equals width w2.

143. according to the pump one of claim 141-142 Suo Shu, wherein, at least two discontinuous passages have height h1 and height h2, wherein height h1 is at least 75% of height h2,90% of height h2,95% of height h2,97.5% of height h2,99% of height h2, or essence equals height h2.

144. according to the pump one of claim 141-143 Suo Shu, and wherein, at least two discontinuous passages have a1He cross-section, cross-section a2, and wherein cross-section a1 is at least 75% of cross-section a2,90% of cross-section a2, and 95% of cross-section a2,

97.5% of cross-section a2,99% of cross-section a2, or essence equals cross-section a2.

145. according to the pump one of claim 141-144 Suo Shu, and wherein, at least two discontinuous passage essence are parallel and concentric.

146. according to the pump described in claim 145, and wherein, at least one passage has radius r 1, and the radius of another one passage is r2, and wherein, r1 is greater than r2.

147. according to the pump described in claim 146, and wherein, radius r 1 is about 1.25 times of radius r 2, and about 1.5 times, about 2 times, about 3 times, about 5 times, about 10 times.

148. according to the pump one of claim 141-147 Suo Shu, and wherein, point is at least two discontinuous passage rotations with one heart around one for break, and the department of the second described basic unit is for compressing in groove.

149. according to the pump described in claim 148, and wherein, break is columniform.

150. according to the pump one of claim 148-149 Suo Shu, and wherein, break comprises at least one element, and this element rotates on the essence axle parallel with the first substrate surface (top).

151. according to the pump one of claim 149-150 Suo Shu, and wherein, break comprises along axle having at least one, and at least two, at least three, at least four, at least five elements independently.

152. according to the pump described in claim 148, and wherein, break is conical.

153. according to the pump described in claim 148-152, and wherein, break, for to have radius r 1 in narrow one end, has radius r 2. in wide one end

154. according to the pump one of claim 148 or 152-153 Suo Shu, and wherein, break is for rotating with the nonparallel axle of the first and second substrate surface along the river.

155. according to the pump one of claim 148 or 152-154 Suo Shu, wherein, in the second basic unit on break at least one passage at least two discontinuous passages, exerts pressure.

156. according to the pump one of claim 149-155 Suo Shu, and wherein, described cylindrical body break has neat edge contour.

157. according to the pump one of claim 149-156 Suo Shu, and wherein, described cylindrical body break has sharp profile, and this profile comprises the structure of the one or more projections corresponding with one or more grooves in the first matrix.

158. according to the pump described in claim 144, wherein, region, cross-section a1 is approximately 1.25 times of region, cross-section a2, for 1.5 times of region, cross-section a2, be 1.75 times of region, cross-section a2, be 2 times of region, cross-section a2, for 4 times of region, cross-section a2, for 6 times of region, cross-section a2, or, be 10 times of region, cross-section a2.

159. according to the pump described in claim 144, wherein, the break rotating completely for each, the fluid of certain volume is assigned as v1, v2 by least two discrete micro passages, wherein, volume v1 is at least about 75%, at least about 90%, at least about 95% of volume v2, at least about 97.5%, or essence is identical with volume v2.

160. according to the pump described in claim 144, wherein, and the break rotating completely for each, the fluid of certain volume is assigned as v1 by least two discrete micro passages, v2, wherein, volume v1 is at least 2 times of volume v2, at least 3 times, at least 5 times, at least 10 times, at least 25 times, at least 50 times, or at least 100 times.

161. microfluidic devices, comprising:

The pump as described in claim 1-144 at least;

The serial microfluidic channel being communicated with at least described pump fluid;

At least one sample applies region;

Surveyed area; With

Waste collection region.

162. micro-fluid pump modules comprise:

(a) the first matrix, comprises the large surface that limits groove thereon;

(b) the second matrix, the second matrix coordinates with the first matrix, carrys out enclosed slot and forms micro passage:

(c) be set to the break of exerting pressure to the second matrix: it is characterized in that, when a pressure is applied in the second matrix, a part for the second matrix allows groove sealing, and under these circumstances, the size of groove is material change not.

163. will go the micro-fluid pump module described in 162 according to right, and wherein, the second matrix has the shore hardness D of 80-90D.

164. according to the micro-fluid pump module described in claim 162 or 163, and wherein, the second matrix has the shore hardness A of 35-55A.

165. according to the micro-fluid pump module described in claim 162-164, wherein, when break is when oppressing the second matrix in the groove in the first matrix, the width w of groove be at least the second matrix do not have the groove under pressure width 75%, be at least 80%, be at least 85%, be at least 90%, be at least 95%, be at least 97.5%, be at least 99%, be at least 99.99%, or essence is the same with the width of groove.

166. according to the micro-fluid pump module described in claim 162-165, wherein, when break is when oppressing the second matrix in the groove in the first matrix, the height h of groove be at least the second matrix do not have the groove under pressure height 75%, be at least 80%, be at least 85%, be at least 90%, be at least 95%, be at least 97.5%, be at least 99%, be at least 99.99%, or essence is the same with the height of groove.

167. according to the micro-fluid pump module described in aforementioned claim, and wherein, the first matrix is chosen from following employing, but is not limited to, polystyrene, polypropylene, polymethylmethacrylate, polycarbonate (PC) or like that.