CN104198648A - Technique for detecting nitrogen in livestock pig excrement - Google Patents

Abstract

The invention discloses a technique for detecting nitrogen in livestock pig excrement. The technique for detecting nitrogen in livestock pig excrement comprises the following steps: (1) taking 10g of air-dried pig excrement and removing impurities on the surface of the excrement by a filter; (2) putting the excrement at the bottom of a flask, adding sulfuric acid and hydrogen peroxide, and standing overnight; (3) heating the mixed excrement for multiple times, adding hydrogen peroxide for two times during the heating of the excrement, then adding the excrement into a volumetric flask, adding water, and standing for clarifying for later use; (4) sucking sterilized and boiled clear liquid into a distilling flask, adding water, a boric acid solution and a sodium hydroxide solution, and then heating for distilling; and (5) titrating a distillate with a hydrochloric acid standard solution until the distillate is just changed from blue to amaranth, and then recording the volume of the consumed acid standard solution. By virtue of accurate operations of the five steps, the method is capable of ensuring that the finally obtained result of the content of nitrogen is relatively accurate; meanwhile, the method is simple and easy to operate, thus being worthy of wide popularization.

Description

The detection technique of nitrogen in a kind of livestock and poultry swine excrement

Technical field

The present invention relates to the detection technique of nitrogen in a kind of ight soil, relate in particular to the detection technique of nitrogen in a kind of swine excrement.

Background technology

The content of nitrogen in feces of livestock and poultry, is assessment " poultry ground balance ", and science is used the important parameter of feces of livestock and poultry fertilizer, also can provide Technical Reference for the utilization of feces of livestock and poultry matrix simultaneously.The nitrogen detection means that is used at present feces of livestock and poultry is all more coarse and very loaded down with trivial details, accordingly, the result detecting and precision are not very satisfactory yet, naturally, for assessment of " poultry ground balance ", the reliability of setting up the optimum ecological circulation of plant husbandry aquaculture is just very low, is also very little to the help of feces of livestock and poultry fertilizer and matrix utilization simultaneously.

Summary of the invention

Technical matters to be solved by this invention is, the detection method of nitrogen in a kind of simple to operation and swine excrement that precision is high is provided, and described method specifically comprises the following steps:

(1) get air-dry swine excrement 10g, utilize the impurity on filtrator filtering ight soil surface;

(2) ight soil is positioned over to drag, adds 10ml sulfuric acid and 3.5ml hydrogen peroxide, bottleneck is placed a curved neck funnel, and placement is spent the night;

(3) mixed ight soil being slowly warming up to sulfuric acid smolders, take off after slightly cold and add 10 hydrogen peroxide, shaking flasks, continue heating takes off for 12 minutes, slightly cold rear gradation adds that 10-20 drips hydrogen peroxide and medicine for treating comedo boils again, until solution be look for or faint yellow clear liquid after, continue heating 15 minutes, eliminate remaining hydrogen peroxide, take off slightly cold, the 50-60ml that adds water, be heated to boiling, take off cooling, rinse curved neck funnel with a small amount of water, in washing lotion income flask, will disappear and boil liquid and put into 300ml volumetric flask, add water and leave standstill clarification, for subsequent use;

(4) absorption disappears and boils clear liquid 40ml in cucurbit, add 150ml water, add 20ml BAS and 10 mixed indicators to be undertaken in condenser pipe lower end in 300ml triangular flask, the mouth of pipe inserts in boric acid liquid level, in cucurbit, add 20ml sodium hydroxide solution by cartridge type funnel, then add; Thermal distillation, liquid to be distillated amasss about 200ml, stops distillation;

(5) with hydrochloric acid standard solution titration distillate, just having faded to aubergine by blueness is terminal, then records the volume of consumption acids standard solution.

(6) use formula $\left(N\right)(\%)=\frac{(V-V_0)\×c\×0.014\×D}{m\×(1-X_0)}\×100$ Calculate nitrogen content.

In formula, V is the volume of test solution titration quota of expenditure acid solution, and unit is milliliter;

V0 is the volume of blank titration quota of expenditure acid solution, and unit is milliliter;

C is the concentration of sour standard solution, and unit is mole every liter;

0.014 is the molal weight of nitrogen, and unit is gram every mole;

D is for point getting multiple, constant volume/point get volume, 100/50;

M is for taking sample mass, and unit is gram;

X0 is the water cut of air-dried sample.

Adopt after above-mentioned fabrication techniques method, the invention has the advantages that, by the accurate operation of 5 steps in the morning, can guarantee that the result of the nitrogen content of finally measuring is more accurate, and method of operating is also simple, is therefore worth promoting.

Embodiment

Below the present invention is described in more detail.

A detection technique for nitrogen in swine excrement, its concrete steps are:

(1) get air-dry swine excrement 10g, utilize the impurity on filtrator filtering ight soil surface;

(2) ight soil is positioned over to drag, adds 10ml sulfuric acid and 3.5ml hydrogen peroxide, bottleneck is placed a curved neck funnel, and placement is spent the night;

(3) mixed ight soil being slowly warming up to sulfuric acid smolders, take off after slightly cold and add 10 hydrogen peroxide, shaking flasks, continue heating takes off for 12 minutes, slightly cold rear gradation adds that 10-20 drips hydrogen peroxide and medicine for treating comedo boils again, until solution be look for or faint yellow clear liquid after, continue heating 15 minutes, eliminate remaining hydrogen peroxide, take off slightly cold, the 50-60ml that adds water, be heated to boiling, take off cooling, rinse curved neck funnel with a small amount of water, in washing lotion income flask, will disappear and boil liquid and put into 300ml volumetric flask, add water and leave standstill clarification, for subsequent use;

(4) absorption disappears and boils clear liquid 40ml in cucurbit, add 150ml water, add 20ml BAS and 10 mixed indicators to be undertaken in condenser pipe lower end in 300ml triangular flask, the mouth of pipe inserts in boric acid liquid level, in cucurbit, add 20ml sodium hydroxide solution by cartridge type funnel, then add; Thermal distillation, liquid to be distillated amasss about 200ml, stops distillation;

(5) with hydrochloric acid standard solution titration distillate, just having faded to aubergine by blueness is terminal, then records the volume of consumption acids standard solution.

(6) use formula $\left(N\right)(\%)=\frac{(V-V_0)\×c\×0.014\×D}{m\×(1-X_0)}\×100$ Calculate nitrogen content.

In formula, V is the volume of test solution titration quota of expenditure acid solution, and unit is milliliter;

V0 is the volume of blank titration quota of expenditure acid solution, and unit is milliliter;

C is the concentration of sour standard solution, and unit is mole every liter;

0.014 is the molal weight of nitrogen, and unit is gram every mole;

D is for point getting multiple, constant volume/point get volume, 100/50;

M is for taking sample mass, and unit is gram;

X0 is the water cut of air-dried sample.

Claims (1)

1. a detection technique for nitrogen in swine excrement, is characterized in that, its concrete steps are:

(1) get air-dry swine excrement 10g, utilize the impurity on filtrator filtering ight soil surface;

(2) ight soil is positioned over to drag, adds 10ml sulfuric acid and 3.5ml hydrogen peroxide, bottleneck is placed a curved neck funnel, and placement is spent the night;

(3) mixed ight soil being slowly warming up to sulfuric acid smolders, take off after slightly cold and add 10 hydrogen peroxide, shaking flasks, continue heating takes off for 12 minutes, slightly cold rear gradation adds that 10-20 drips hydrogen peroxide and medicine for treating comedo boils again, until solution be look for or faint yellow clear liquid after, continue heating 15 minutes, eliminate remaining hydrogen peroxide, take off slightly cold, the 50-60ml that adds water, be heated to boiling, take off cooling, rinse curved neck funnel with a small amount of water, in washing lotion income flask, will disappear and boil liquid and put into 300ml volumetric flask, add water and leave standstill clarification, for subsequent use;

(4) absorption disappears and boils clear liquid 40ml in cucurbit, add 150ml water, add 20ml BAS and 10 mixed indicators to be undertaken in condenser pipe lower end in 300ml triangular flask, the mouth of pipe inserts in boric acid liquid level, in cucurbit, add 20ml sodium hydroxide solution by cartridge type funnel, then add; Thermal distillation, liquid to be distillated amasss about 200ml, stops distillation;

(5) with hydrochloric acid standard solution titration distillate, just having faded to aubergine by blueness is terminal, then records the volume of consumption acids standard solution;

(6) use formula $\left(N\right)(\%)=\frac{(V-V_0)\×c\×0.014\×D}{m\×(1-X_0)}\×100$ Calculate nitrogen content,

In formula, V is the volume of test solution titration quota of expenditure acid solution, and unit is milliliter;

V0 is the volume of blank titration quota of expenditure acid solution, and unit is milliliter;

C is the concentration of sour standard solution, and unit is mole every liter;

0.014 is the molal weight of nitrogen, and unit is gram every mole;

D is for point getting multiple, constant volume/point get volume, 100/50;

M is for taking sample mass, and unit is gram;

X0 is the water cut of air-dried sample.