CN107764782A - The method that Accurate Determining can be quickly carried out to Arsenic in Drinking Water content - Google Patents
The method that Accurate Determining can be quickly carried out to Arsenic in Drinking Water content Download PDFInfo
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- CN107764782A CN107764782A CN201610690309.7A CN201610690309A CN107764782A CN 107764782 A CN107764782 A CN 107764782A CN 201610690309 A CN201610690309 A CN 201610690309A CN 107764782 A CN107764782 A CN 107764782A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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Abstract
The invention discloses a kind of method that Accurate Determining can be quickly carried out to Arsenic in Drinking Water content, it is accurate to draw 25ml samples, inject in 100ml conical flasks, 2ml salpeter solutions are added, small funnel are inserted, as in sand bath, sample is set to be incubated 30min under near boiling state in solution, conical flask cooling is taken out, solution is then transferred to 50ml volumetric flasks, adds distillation aqueous to graticule；Take 50mL water samples to add in 100mL conical flasks, add 2mL nitric acid, be put into sand-bath and clear up, arrive 10mL until clearing up, be transferred to 25mL volumetric flasks, add water constant volume.Taken with distilled water and operated in right amount instead of sample by above-mentioned steps.Add 50% hydrochloric acid solution 10.0mL, the ascorbic acid mixed solution 5.0mL of thiocarbamide one successively to sample and graticule, constant volume simultaneously shakes up, and at least places 15min, to be measured, as less than 15 DEG C placement 30min of room temperature are to be measured；As a result calculate.This method can measure the content of Arsenic in Drinking Water exactly, and continuous mode is easy, ensure that the safety that people drink.
Description
Technical field
The present invention relates to a kind of method, and quickly Accurate Determining can be carried out to Arsenic in Drinking Water content more particularly, to one kind
Method.
Background technology
Drinking water refers to can be without handling, directly feed the water that human body drinks.Water is the chief component of body fluid, is
Form the important substance of cell, tissue fluid, blood plasma etc..Water as in vivo all chemical reaction media, be various nutrients and
The platform of matter transportation.Drinking water is packed with its 40% occupation rate of market, has been sure to occupy the national beverage market first.It is expert at
In industry, manufacturing enterprise's accounting weight of pure water, natural mineral water and mineral water is maximum.Consumer to the preference of drinking water
Change, corporate strategy emphasis also follows market to be transferred to natural mineral water.The 1-12 months in 2011, whole nation packaging drinking water
About 47,890,000 tons of yield, increased by 25.67% than 2010.On March 17th, 2014, Chinese Ministry of Environmental Protection issue result of study show, China
There are 2.8 hundred million residents to use dangerous drinking water.
Entitled " research of the Chinese population environmental exposure behavior pattern " result of study of Chinese Ministry of Environmental Protection's issue is shown：China has 1.1
There is the blowdown enterprise that petrochemical industry, coking, thermal power generation etc. are paid close attention in the kilometer range of hundred million residential building periphery 1,2.8 hundred million residents make
With dangerous drinking water, the use that should speed up to live can clean and high quality, accelerates drinking water safety transformation.Arsenic, arsenic is commonly called as,
A kind of nonmetalloid, in the periodic table of chemical element positioned at the 4th cycle, V A races, atomic number 33, symbol of element As,
Simple substance exists in the form of grey arsenic, black arsenic and yellow arsenic these three allotropes.Arsenic element widely exists in nature, shares
Hundreds of arsenic minerals is to have been observed that.Arsenic is used in agricultural chemicals, herbicide, insecticide with its compound, the conjunction with many kinds
Jin Zhong.In ancient times, arsenic trioxide is referred to as arsenic, but a small amount of arsenic is beneficial to body.For the content of arsenic in drinking water
There is strict regulation, when the content of arsenic is above standard value, there will be very big injury to human body, it is existing in drinking water
The assay method process of arsenic content is complicated, and the resultant error measured is very big, causes potential safety hazard be present to human body.
The content of the invention
It is an object of the invention to overcome the above-mentioned existing assay method process for Arsenic in Drinking Water content complicated, and
The resultant error measured is very big, causes the problem of potential safety hazard be present to human body, and devising one kind can be quickly in drinking water
The method that arsenic content carries out Accurate Determining, this method can measure the content of Arsenic in Drinking Water, and continuous mode letter exactly
Just, the safety that people drink is ensure that, solves the existing assay method process complexity for Arsenic in Drinking Water content, and survey
The resultant error obtained is very big, causes the problem of potential safety hazard be present to human body.
The purpose of the present invention is achieved through the following technical solutions：Accurate Determining quickly can be carried out to Arsenic in Drinking Water content
Method, comprise the following steps：
（1）Analysis process：The preservation and pretreatment of water sample：It is accurate to draw 25ml samples, inject in 100ml conical flasks, add 2ml
Salpeter solution（1+1）, small funnel is inserted, as in sand bath, making sample be incubated 30min under near boiling state in solution, takes out cone
Shape bottle is cooled down, and solution then is transferred into 50ml volumetric flasks, adds distillation aqueous to shake to graticule, to be measured, is settled to 0.2% nitric acid
Blank sample is made in 50mL volumetric flasks, it is to be measured；
The preparation of standard working curve：Arsenic standard solution 1mL is taken, constant volume to 25mL, instrument automatic dilution, draws graticule；
Determination sample：Take 50mL water samples to add in 100mL conical flasks, add 2mL（1:1）Nitric acid, be put into sand-bath and clear up, until
10mL is cleared up, is transferred to 25mL volumetric flasks, adds water constant volume.Taken with distilled water and operated in right amount instead of sample by above-mentioned steps, surveyed
Determine blank sample, add 50% hydrochloric acid solution 10.0mL, the ascorbic acid mixed solution 5.0mL of thiocarbamide one successively to sample and graticule, it is fixed
Hold and shake up, at least place 15min, it is to be measured, as room temperature less than 15 DEG C place 30min it is to be measured, according to instrumentation code preheat
30min, connect source of the gas, adjust outlet pressure, using 10% hydrochloric acid solution as current-carrying, instrument is adjusted by Instrument working parameter
Device, arsenic standard working curve is determined, the standard working curve coefficient correlation of measure should be greater than 0.9990, and otherwise should ascertain the reason weight
New bioassay standard curve presses foregoing mensuration program, first determination sample blank with rule of three processing data, then is surveyed successively by program
Determine each sample concentration；
（2）As a result calculate：Instrument accompanying software has the function of calculating automatically, and working curve is linear fit curve, and measure is to be measured
Sample blank fluorescence intensity is subtracted after fluorescent intensity level, for the linear function of people's matched curve, that is, show that testing sample is dense
Degree, if artificial calculate, formula can be used：
In formula: CAs=C×V/V0
CAs --- testing sample concentration, unit are micro- gram per liter (μ g/mL)；
C --- after subtracting sample blank fluorescence intensity according to the fluorescence intensity of testing sample, checked in from working curve corresponding
Sample concentration；
Final volume of V --- the testing sample after handling, dilute constant volume；
V0 --- take the volume of testing sample.
In summary, the beneficial effects of the invention are as follows：This method can measure the content of Arsenic in Drinking Water exactly, and
Continuous mode is easy, ensure that the safety that people drink, solves the existing assay method process for Arsenic in Drinking Water content
Complexity, and the resultant error measured is very big, causes the problem of potential safety hazard be present to human body.
Embodiment
With reference to embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited only to
This.
Embodiment：
The method that Accurate Determining can be quickly carried out to Arsenic in Drinking Water content, comprises the following steps：
（1）Analysis process：The preservation and pretreatment of water sample：It is accurate to draw 25ml samples, inject in 100ml conical flasks, add 2ml
Salpeter solution（1+1）, small funnel is inserted, as in sand bath, making sample be incubated 30min under near boiling state in solution, takes out cone
Shape bottle is cooled down, and solution then is transferred into 50ml volumetric flasks, adds distillation aqueous to shake to graticule, to be measured, is settled to 0.2% nitric acid
Blank sample is made in 50mL volumetric flasks, it is to be measured；
The preparation of standard working curve：Arsenic standard solution 1mL is taken, constant volume to 25mL, instrument automatic dilution, draws graticule；
Determination sample：Take 50mL water samples to add in 100mL conical flasks, add 2mL（1:1）Nitric acid, be put into sand-bath and clear up, until
10mL is cleared up, is transferred to 25mL volumetric flasks, adds water constant volume, is taken with distilled water and is operated in right amount instead of sample by above-mentioned steps, is surveyed
Determine blank sample, add 50% hydrochloric acid solution 10.0mL, the ascorbic acid mixed solution 5.0mL of thiocarbamide one successively to sample and graticule, it is fixed
Hold and shake up, at least place 15min, it is to be measured, as room temperature less than 15 DEG C place 30min it is to be measured, according to instrumentation code preheat
30min, connect source of the gas, adjust outlet pressure, using 10% hydrochloric acid solution as current-carrying, instrument is adjusted by Instrument working parameter
Device, arsenic standard working curve is determined, the standard working curve coefficient correlation of measure should be greater than 0.9990, and otherwise should ascertain the reason weight
New bioassay standard curve presses foregoing mensuration program, first determination sample blank with rule of three processing data, then is surveyed successively by program
Determine each sample concentration；
（2）As a result calculate：Instrument accompanying software has the function of calculating automatically, and working curve is linear fit curve, and measure is to be measured
Sample blank fluorescence intensity is subtracted after fluorescent intensity level, for the linear function of people's matched curve, that is, show that testing sample is dense
Degree, if artificial calculate, formula can be used：
In formula: CAs=C×V/V0
CAs --- testing sample concentration, unit are micro- gram per liter (μ g/mL)；
C --- after subtracting sample blank fluorescence intensity according to the fluorescence intensity of testing sample, checked in from working curve corresponding
Sample concentration；
Final volume of V --- the testing sample after handling, dilute constant volume；
V0 --- take the volume of testing sample.
This method can measure the content of Arsenic in Drinking Water exactly, and continuous mode is easy, ensure that people drink
Safety, it is complicated to solve the existing assay method process for Arsenic in Drinking Water content, and the resultant error measured is very big,
Cause the problem of potential safety hazard be present to human body.
It is described above, be only presently preferred embodiments of the present invention, any formal limitation not done to the present invention, it is every according to
Any simply modification, the equivalent variations substantially made according to the technology of the present invention, method to above example, each fall within the present invention
Protection domain within.
Claims (1)
- The method that 1. Accurate Determining can be quickly carried out to Arsenic in Drinking Water content, it is characterised in that comprise the following steps：（1）Analysis process：The preservation and pretreatment of water sample：It is accurate to draw 25ml samples, inject in 100ml conical flasks, add 2ml Salpeter solution（1+1）, small funnel is inserted, as in sand bath, making sample be incubated 30min under near boiling state in solution, takes out cone Shape bottle is cooled down, and solution then is transferred into 50ml volumetric flasks, adds distillation aqueous to shake to graticule, to be measured, is settled to 0.2% nitric acid Blank sample is made in 50mL volumetric flasks, it is to be measured；The preparation of standard working curve：Arsenic standard solution 1mL is taken, constant volume to 25mL, instrument automatic dilution, draws graticule；Determination sample：Take 50mL water samples to add in 100mL conical flasks, add 2mL（1:1）Nitric acid, be put into sand-bath and clear up, until 10mL is cleared up, is transferred to 25mL volumetric flasks, adds water constant volume, is taken with distilled water and is operated in right amount instead of sample by above-mentioned steps, is surveyed Determine blank sample, add 50% hydrochloric acid solution 10.0mL, the ascorbic acid mixed solution 5.0mL of thiocarbamide one successively to sample and graticule, it is fixed Hold and shake up, at least place 15min, it is to be measured, as room temperature less than 15 DEG C place 30min it is to be measured, according to instrumentation code preheat 30min, connect source of the gas, adjust outlet pressure, using 10% hydrochloric acid solution as current-carrying, instrument is adjusted by Instrument working parameter Device, arsenic standard working curve is determined, the standard working curve coefficient correlation of measure should be greater than 0.9990, and otherwise should ascertain the reason weight New bioassay standard curve presses foregoing mensuration program, first determination sample blank with rule of three processing data, then is surveyed successively by program Determine each sample concentration；（2）As a result calculate：Instrument accompanying software has the function of calculating automatically, and working curve is linear fit curve, and measure is to be measured Sample blank fluorescence intensity is subtracted after fluorescent intensity level, for the linear function of people's matched curve, that is, show that testing sample is dense Degree, if artificial calculate, formula can be used：In formula: CAs=C×V/V0CAs --- testing sample concentration, unit are micro- gram per liter (μ g/mL)；C --- after subtracting sample blank fluorescence intensity according to the fluorescence intensity of testing sample, checked in from working curve corresponding Sample concentration；Final volume of V --- the testing sample after handling, dilute constant volume；V0 --- take the volume of testing sample.
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| CN201610690309.7A CN107764782A (en) | 2016-08-20 | 2016-08-20 | The method that Accurate Determining can be quickly carried out to Arsenic in Drinking Water content |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108489955A (en) * | 2018-05-18 | 2018-09-04 | 江苏省农业科学院 | A kind of non-chromatographic detection method detecting four kinds of arsenic morphologies in rice flour based on HG-AFS |
| CN108776125A (en) * | 2018-08-03 | 2018-11-09 | 贵州省疾病预防控制中心 | A kind of sample pre-treatments reagent and method measured in urine during arsenic |
-
2016
- 2016-08-20 CN CN201610690309.7A patent/CN107764782A/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108489955A (en) * | 2018-05-18 | 2018-09-04 | 江苏省农业科学院 | A kind of non-chromatographic detection method detecting four kinds of arsenic morphologies in rice flour based on HG-AFS |
| CN108489955B (en) * | 2018-05-18 | 2020-11-06 | 江苏省农业科学院 | Non-chromatographic detection method for detecting four arsenic forms in rice flour based on HG-AFS |
| CN108776125A (en) * | 2018-08-03 | 2018-11-09 | 贵州省疾病预防控制中心 | A kind of sample pre-treatments reagent and method measured in urine during arsenic |
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Application publication date: 20180306 |