CN104186436A - Method and device for detecting feeding characteristics of insects - Google Patents
Method and device for detecting feeding characteristics of insects Download PDFInfo
- Publication number
- CN104186436A CN104186436A CN201410461483.5A CN201410461483A CN104186436A CN 104186436 A CN104186436 A CN 104186436A CN 201410461483 A CN201410461483 A CN 201410461483A CN 104186436 A CN104186436 A CN 104186436A
- Authority
- CN
- China
- Prior art keywords
- chamber
- food
- insect
- cavity
- detected
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Sampling And Sample Adjustment (AREA)
Abstract
The invention provides a method and device for detecting the feeding characteristics of insects. The device comprises a shell, wherein the bottom of the shell is closed, the shell is internally provided with a cavity, and the top of the shell is provided with a movable cover. The device is characterized in that partition boards separating the cavity into multiple chambers are arranged in the cavity; the bottoms of the partition boards are provided with through grooves, and the chambers are communicated in pairs through the through grooves, so that the chambers which can be separated from one another and communicated with one another in an adjacent mode are formed in the cavity; a test insect is placed in one chamber which is separated from and communicated with the other chambers, and the test insect will pass through the through grooves in the bottoms of the partition boards between the chambers and go to the separated chambers freely to eat detected food; after a set feeding time, data of preferences, recipes (host specificity detection), feeding amount, feeding frequency and the like of the test insect on a certain kind of food to be detected can be obtained, and the feeding characteristics of the test insect are obtained ultimately. The method and device are simple, convenient to use and easy to implement.
Description
Technical field
The present invention relates to a kind of method and device that detects insect's food-taking characteristic, belong to the detection of insect feeding habits, studying technological domain.
Background technology
The method of research insect's food-taking characteristic has varied, has developed into now molecular biology level.Conventional art as research object, the food pedigree of a certain insect of system research mainly with single insect, single diet, and not only working procedure numerous and complicated, and workload is very big, and the time of expending is longer.Based on this, study and develop and can grasp simply, directly, easily the method for insect's food-taking characteristic and install very important.
At present studying relevant patent from insect feeding habits only has " different host population Batocera horsfieldi larval feedings selection finders (CN201320521732) " one.The present invention by comparison structure simpler, use more conveniently, and can be used for the synchronous detection of various insects feeding habits.
Summary of the invention
The object of the present invention is to provide a kind of device that detects insect's food-taking characteristic.
The object of the present invention is to provide a kind of method of utilizing the device of detection insect's food-taking characteristic to carry out insect's food-taking Characteristics Detection.
The device of detection insect's food-taking characteristic provided by the invention, comprise bottom lock, the interior housing with cavity, top band removable cover, it is characterized in that being provided with in cavity the dividing plate that cavity is divided into multiple chambers, described dividing plate bottom is provided with and connects the groove of chamber between two, makes cavity form multiple chambers of not only isolating but also can be communicated with adjacent chamber.
Described housing is made as cylinder or polygonal cylinder, and the cavity section in it is made as circle or polygon.
Described dividing plate is two blocks of straight plates that right-angled intersection arranges, be placed in circular cavity and form four fan-shaped chambers, or be placed in rectangular enclosure and form four rectangular chamber, and often always bottom, plate two ends be provided with groove, chamber is between two connected.
Described dividing plate is made as at least three blocks of straight plates, be placed in polygon cavity and form the chamber of multiple rectangles, the mixing of multiple triangle or form multiple triangular chamber, and the bottom of the dividing plate between chamber is provided with the groove that allows chamber mutually connect between two.
Described dividing plate is made as the circular slab at middle part and the straight plate of periphery, being placed in circular cavity formation middle part is the mixing chamber that a circular chamber, periphery are multiple fan-shaped chambers, or being placed in polygon cavity formation middle part is a circular chamber, the mixing chamber that periphery is multiple shaped chamber.
Described each chamber area is identical or not identical.
A kind of method of utilizing insect's food-taking characteristic detection device to detect insect's food-taking characteristic provided by the invention, is characterized in that comprising the following steps:
1) the different foods to be detected of setting weight are put in respectively in the chamber of not only isolating but also being communicated with adjacent chamber;
2) test insect is put in and step 1) the adjacent vacant chamber of the chamber that is placed with food to be detected in, allow the groove of the dividing plate bottom that insect passes through to arrange between chamber, between the chamber that is placed with different foods to be detected, freely walk to setting-up time;
3) arrived after setting-up time, according to the quantity in the chamber of the corresponding food to be detected in test insect place and the expendable weight of food to be detected, obtain hobby, the appetite of this test insect to a certain food to be detected, take food frequency, thereby obtain testing the food species that takes food characteristic and like of insect.
The present invention has following advantages and effect: adopt such scheme, can be easily test insect be placed in and be not only isolated but also a certain chamber of UNICOM mutually, make to test the chamber that insect can freely navigate within each isolation by the groove of dividing plate bottom between chamber and take food detected food, through the time that takes food of setting, can draw hobby, recipe (host specificity detections), the food ingestion of test insect to a certain food to be detected, take food the data such as frequency, the final characteristic that takes food of testing insect that obtains.The present invention is simple, convenient, Yi Hang.
Brief description of the drawings
Fig. 1 is apparatus of the present invention longitudinal section;
Fig. 2 is the A-A view of Fig. 1;
Fig. 3 is another cross-section structure of the present invention;
Fig. 4 is the another cross-section structure of the present invention;
Fig. 5 is the present invention's cross-section structure again;
Fig. 6 is the another structure chart that is broken into of the present invention.
Embodiment
Below in conjunction with drawings and Examples, the present invention is described further.
The device of detection insect's food-taking characteristic provided by the invention comprises bottom lock, interior band cavity 2, the housing 1 of top band removable cover 4, and be located at the dividing plate 3 that cavity is divided into multiple chambers in cavity 2, with the perforation groove 5 of chamber between two of being located at dividing plate 3 bottoms, make cavity 2 form multiple chambers of not only isolating but also can be communicated with adjacent chamber, described housing 1 is made as rectangular cylinder, cavity Cross-Section Rectangular in it, two blocks of straight plates that described dividing plate 3 arranges for right-angled intersection, be placed in rectangular enclosure and form four rectangular chamber 6, and often bottom, plate two ends is provided with groove 5 always, chamber is between two connected, four chamber area are identical, as Fig. 1, Fig. 2, two blocks of straight plates that described dividing plate 3 arranges for right-angled intersection, be placed in circular cavity and form four fan-shaped chambers 7, and often bottom, plate two ends is provided with groove 5 always, chamber is between two connected, four chamber area are identical, as Fig. 3,
Described dividing plate is made as the circular slab 31 at middle part and the straight plate 3 of periphery, the hexagonal cavities formation middle part that is placed in hexagon housing 12 is a circular chamber 8, periphery is the mixing chamber of six shaped chamber 9, and the bottom of the dividing plate between chamber is provided with the groove 5 that allows chamber mutually connect between two, seven chamber area are not identical, as Fig. 4.
Described dividing plate is made as three blocks of straight plates 3, the pentagon cavity that is placed in pentagon housing 13 forms the mixing chamber of two rectangular chamber 6, four triangular chamber 10, and it is not identical bottom the dividing plate between chamber, to be provided with 5, six chamber area of groove that allow chamber mutually connect between two, as Fig. 5.
Described dividing plate is made as three blocks of straight plates 3, and the pentagon cavity that is placed in pentagon housing 13 forms five triangular chamber 10, and to be provided with 5, five chamber area of groove that allow chamber mutually connect not identical the dividing plate between chamber bottom between two, as Fig. 6.
Embodiment 1
The method of utilizing the device of the detection insect's food-taking characteristic that Fig. 3 provides to detect test insect's food-taking characteristic, comprises the following steps:
1) two kinds of different foods to be detected of setting weight are put in respectively in chamber B, the D that not only isolates but also can be communicated with adjacent chamber;
2) same being tested to insect is put in and step 1) the adjacent vacant cavity C of the chamber B, the D that are placed with food to be detected, E in, allow test insect by the groove 5 of dividing plate 3 bottoms that arrange between chamber, between the chamber B, the D that are placed with two kinds of difference foods to be detected, freely walk to setting-up time;
3) arrived after setting-up time, according to the quantity in cavity C, the E of the corresponding food to be detected in test insect place and the expendable weight of food to be detected, obtain hobby, the appetite of this test insect to a certain food to be detected, take food frequency, thereby obtain testing the food species that takes food characteristic and like of insect.
Embodiment 2
The method of utilizing the device of the detection insect's food-taking characteristic that Fig. 4 provides to detect test insect's food-taking characteristic, comprises the following steps:
1) six kinds of different foods to be detected of setting weight are put in respectively in six chambers 9 not only isolating but also can be communicated with adjacent chamber;
2) test insect is put in and step 1) the adjacent circular chamber 8 of the chamber that is placed with food to be detected 9 in, allow test insect by the groove 5 of dividing plate 3 bottoms that arrange between chamber, between the chamber 9 that is placed with six kinds of difference foods to be detected, freely walk to setting-up time;
3) arrived after setting-up time, according to the quantity in the chamber of the corresponding food to be detected in test insect place and the expendable weight of food to be detected, obtain hobby, the appetite of this test insect to a certain food to be detected, take food frequency, thereby obtain testing the food species that takes food characteristic and like of insect.
For showing that the present invention detects the effect of insect feeding habits, is proved below by concrete test.
1, materials and methods
1.1 Mikania micrantha neck fleahoppers take food Characteristics Detection test
Mikania micrantha neck fleahopper (Pachypeltis sp.) is the Mikania micrantha Important Natural Enemy insect of inventor's early detection, but it is blank that its feeding habit and safety research work thereof still belong to, only there is field investigation result of the test, there is no indoor demonstration test data, be difficult to the feeding habits feature of explanation Mikania micrantha neck fleahopper.
Therefore, the inventor is in November, 2013, take food on characteristic research basis in Mikania micrantha neck fleahopper field, with Mikania micrantha and Mikania micrantha generating region kindred plant, accompanying plant, and Important Economic crop, landscape garden are afforested 4 class 55 section's 127 Plants as test plant (in table 1).
By Fig. 3 experimental rig provided by the invention, the 2 metafiltration paper that tile in B, C, D, tetra-fan-shaped chamber 7 bottoms of E, dropping water moistens skin, B district puts into test plant young leaflet tablet, and D district, diagonal angle puts into Mikania micrantha blade;
Fang Chong district C, E put into respectively each 10 of health, active Mikania micrantha neck fleahopper adult, after 6,12 and the 24h that set, and statistics B district, D district Mikania micrantha neck fleahopper adult quantity.Test repeats 3 times, is relatively placed with the B district of test plant young leaflet tablet and places the difference between the Mikania micrantha neck fleahopper adult quantity in D district of Mikania micrantha blade, just can determine the characteristic that takes food of Mikania micrantha neck fleahopper adult.
1.2 data processing
Application Excel and SPSS17.0 etc. carry out Oen-Way ANOVA analysis to data, and carry out multiple ratio with the least significant difference (LSD), and statistical significance level is P<0.05.
2 results and analysis
Mikania micrantha neck fleahopper takes food attribute testing and the results are shown in Table 2.
As shown in Table 2,6h, 12h, 24h check B, D sector region Mikania micrantha neck fleahopper insect population quantity, and the insect population quantity that neck fleahopper selects to take food D district Mikania micrantha is all significantly greater than and takes food B district test plant insect population quantity; Except D district is that Mikania micrantha, B district are beggar-ticks, when 6h, D district neck fleahopper insect population quantity is 9.33 ± 1.76, B district neck fleahopper insect population quantity is 6.00 ± 0.57, the each time point of all the other experimental group, and neck fleahopper selects the insect population quantity that takes food Mikania micrantha in D district to be all greater than 10; B region Mikania micrantha neck fleahopper insect population quantity is all less than 5.
3 conclusions
The method of detection insect's food-taking characteristic provided by the invention, detect Mikania micrantha neck fleahopper according to the embodiment of the present invention 1 scheme and take food selectivity, result shows, even if there is the plant phenomenon taking food except Mikania micrantha in Mikania micrantha neck fleahopper, but test 6h, 12h, arbitrary moment of 24h, the neck fleahopper insect population quantity that selection takes food Mikania micrantha is all significantly greater than the insect population quantity of selecting to take food test plant beyond non-host Mikania micrantha, and evidence Mikania micrantha neck fleahopper is had a liking for food Mikania micrantha.
Table 1 test plant
Table 2 Mikania micrantha neck fleahopper imago feeding is selected situation (D district is Mikania micrantha blade)
In table, data are mean value ± standard errors, data acquisition LSD multiple comparison analyse.Colleague compares, the identical expression of lowercase P > 0.05, and difference is not remarkable; Minuscule difference, represents P < 0.05, significant difference.
Claims (7)
1. one kind is detected the device of insect's food-taking characteristic, comprise bottom lock, the interior housing with cavity, top band removable cover, it is characterized in that being provided with in cavity the dividing plate that cavity is divided into multiple chambers, described dividing plate bottom is provided with and connects the groove of chamber between two, makes cavity form multiple chambers of not only isolating but also can be communicated with adjacent chamber.
2. the device of detection insect's food-taking characteristic according to claim 1, is characterized in that described housing is made as cylinder or polygonal cylinder, and the cavity section in it is made as circle or polygon.
3. the device of detection insect's food-taking characteristic according to claim 1, it is characterized in that described dividing plate is two blocks of straight plates that right-angled intersection arranges, be placed in circular cavity and form four fan-shaped chambers, or be placed in rectangular enclosure and form four rectangular chamber, and often bottom, plate two ends is provided with groove always, chamber is between two connected.
4. the device of detection insect's food-taking characteristic according to claim 1, it is characterized in that described dividing plate is made as at least three blocks of straight plates, be placed in polygon cavity and form the chamber of multiple rectangles, the mixing of multiple triangle or form multiple triangular chamber, and the bottom of the dividing plate between chamber is provided with the groove that allows chamber mutually connect between two.
5. the device of detection insect's food-taking characteristic according to claim 1, it is characterized in that described dividing plate is made as the circular slab at middle part and the straight plate of periphery, being placed in circular cavity formation middle part is the mixing chamber that a circular chamber, periphery are multiple fan-shaped chambers, or being placed in polygon cavity formation middle part is a circular chamber, the mixing chamber that periphery is multiple shaped chamber.
6. the device of detection insect's food-taking characteristic according to claim 1, is characterized in that described each chamber area is identical or not identical.
7. a method of utilizing the device that detects insect's food-taking characteristic described in claim 1 to detect insect's food-taking characteristic, is characterized in that comprising the following steps:
1) the different foods to be detected of setting weight are put in respectively in the chamber of not only isolating but also being communicated with adjacent chamber;
2) test insect is put in the vacant chamber adjacent with the chamber that is placed with food to be detected of step 1), allows the groove of the dividing plate bottom that insect passes through to arrange between chamber, between the chamber that is placed with different foods to be detected, freely walk to setting-up time;
3) arrived after setting-up time, according to the quantity in the chamber of the corresponding food to be detected in test insect place and the expendable weight of food to be detected, obtain hobby, the appetite of this test insect to a certain food to be detected, take food frequency, thereby obtain testing the food species that takes food characteristic and like of insect.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410461483.5A CN104186436A (en) | 2014-09-11 | 2014-09-11 | Method and device for detecting feeding characteristics of insects |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410461483.5A CN104186436A (en) | 2014-09-11 | 2014-09-11 | Method and device for detecting feeding characteristics of insects |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104186436A true CN104186436A (en) | 2014-12-10 |
Family
ID=52071956
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410461483.5A Pending CN104186436A (en) | 2014-09-11 | 2014-09-11 | Method and device for detecting feeding characteristics of insects |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104186436A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111066735A (en) * | 2019-12-24 | 2020-04-28 | 深圳市铁汉生态环境股份有限公司 | Feeding device for measuring feeding amount of insects |
| CN112806283A (en) * | 2020-12-30 | 2021-05-18 | 青海保绿丰生态农林科技有限公司 | Device for recording daily catching amount and species data analysis, statistics and monitoring of eagle |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201001335Y (en) * | 2007-01-06 | 2008-01-09 | 王雨淋 | Ant raising device |
| WO2010038054A2 (en) * | 2008-10-01 | 2010-04-08 | Inscentinel Ltd | Insect holder |
| CN201550508U (en) * | 2009-12-01 | 2010-08-18 | 东北农业大学 | Worm rearing box for cultivating soybean pod borers |
| CN202019639U (en) * | 2011-01-04 | 2011-11-02 | 广东省昆虫研究所 | Black solider fly larvae feed separating device |
| CN102578049A (en) * | 2012-02-29 | 2012-07-18 | 成都鼎明光电科技有限公司 | Insect phototaxis testing device |
| CN102845368A (en) * | 2012-10-10 | 2013-01-02 | 江苏省农业科学院 | Method for quickly determining behavior reactions of dipster to edible fungus |
| CN203388113U (en) * | 2013-07-31 | 2014-01-15 | 滨州学院 | Feeding habit selection observation device for batocera horsfieldi adults |
| CN203482771U (en) * | 2013-08-25 | 2014-03-19 | 滨州学院 | Device for observing feeding selection of batocera horsfieldi larvae of different host populations |
| CN204104551U (en) * | 2014-09-11 | 2015-01-21 | 云南省林业科学院 | A kind of device detecting insect's food-taking characteristic |
-
2014
- 2014-09-11 CN CN201410461483.5A patent/CN104186436A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201001335Y (en) * | 2007-01-06 | 2008-01-09 | 王雨淋 | Ant raising device |
| WO2010038054A2 (en) * | 2008-10-01 | 2010-04-08 | Inscentinel Ltd | Insect holder |
| CN201550508U (en) * | 2009-12-01 | 2010-08-18 | 东北农业大学 | Worm rearing box for cultivating soybean pod borers |
| CN202019639U (en) * | 2011-01-04 | 2011-11-02 | 广东省昆虫研究所 | Black solider fly larvae feed separating device |
| CN102578049A (en) * | 2012-02-29 | 2012-07-18 | 成都鼎明光电科技有限公司 | Insect phototaxis testing device |
| CN102845368A (en) * | 2012-10-10 | 2013-01-02 | 江苏省农业科学院 | Method for quickly determining behavior reactions of dipster to edible fungus |
| CN203388113U (en) * | 2013-07-31 | 2014-01-15 | 滨州学院 | Feeding habit selection observation device for batocera horsfieldi adults |
| CN203482771U (en) * | 2013-08-25 | 2014-03-19 | 滨州学院 | Device for observing feeding selection of batocera horsfieldi larvae of different host populations |
| CN204104551U (en) * | 2014-09-11 | 2015-01-21 | 云南省林业科学院 | A kind of device detecting insect's food-taking characteristic |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111066735A (en) * | 2019-12-24 | 2020-04-28 | 深圳市铁汉生态环境股份有限公司 | Feeding device for measuring feeding amount of insects |
| CN112806283A (en) * | 2020-12-30 | 2021-05-18 | 青海保绿丰生态农林科技有限公司 | Device for recording daily catching amount and species data analysis, statistics and monitoring of eagle |
| CN112806283B (en) * | 2020-12-30 | 2022-11-11 | 青海保绿丰生态农林科技有限公司 | Device for recording daily catching amount and species data analysis, statistics and monitoring of eagle |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Pawlik et al. | Sponge communities on Caribbean coral reefs are structured by factors that are top-down, not bottom-up | |
| Goulson | Why do pollinators visit proportionally fewer flowers in large patches? | |
| Jumpponen et al. | Massively parallel 454 sequencing indicates hyperdiverse fungal communities in temperate Quercus macrocarpa phyllosphere | |
| Lumbsch et al. | New higher taxa in the lichen family Graphidaceae (lichenized Ascomycota: Ostropales) based on a three-gene skeleton phylogeny | |
| Gast et al. | Characteristics of Salmonella enteritidis contamination in eggs after oral, aerosol, and intravenous inoculation of laying hens | |
| Brown et al. | A phylogenetic perspective on habitat shifts and diversity in the North American Enallagma damselflies | |
| Vizzini et al. | Paralepistopsis gen. nov. and Paralepista (Basidiomycota, Agaricales) | |
| Oldroyd et al. | Comb architecture of the eusocial bees arises from simple rules used during cell building | |
| Labruna et al. | Description of adults and nymph, and redescription of the larva, of Ornithodoros marinkellei (Acari: Argasidae), with data on its phylogenetic position | |
| Lione et al. | Testing ecological interactions between Gnomoniopsis castaneae and Dryocosmus kuriphilus | |
| CN104186436A (en) | Method and device for detecting feeding characteristics of insects | |
| Buckley et al. | Hexagonal packing of royal tern nests | |
| Das et al. | Morphology and phylogeny of four new Lactarius species from Himalayan India | |
| Baker et al. | Inverse dispersal patterns in a group of ant parasitoids (Hymenoptera: Eucharitidae: Oraseminae) and their ant hosts | |
| Hoyle et al. | Species richness of moss landscapes unaffected by short‐term fragmentation | |
| Aglietti et al. | From chestnut tree (Castanea sativa) to flour and foods: A systematic review of the main criticalities and control strategies towards the relaunch of chestnut production chain | |
| Miko et al. | Taxonomy of European Damaeidae (Acari: Oribatida) I. Kunstidamaeus Miko, 2006, with comments on Damaeus sensu lato | |
| CN204104551U (en) | A kind of device detecting insect's food-taking characteristic | |
| Noraini et al. | Systematic significance of stipe anatomy in peninsular Malaysian Blechnum L.(Blechnaceae) species | |
| Hosen et al. | Amanitatullossiana, a new species, and two new records of AmanitasectionLepidella from north-western Himalaya, India | |
| Potts et al. | Floristic heterogeneity at N gogo, K ibale National Park, U ganda and possible implications for habitat use by chimpanzees (P an troglodytes) | |
| Bowman | Cheliceral chelal design in free-living astigmatid mites | |
| Sosnovsky et al. | Trophic interactions in shallow lakes of the Pampa plain (Argentina) and their effects on water transparency during two cold seasons of contrasting fish abundance | |
| Calvert et al. | The genomics of trait combinations and their influence on adaptive divergence | |
| McArthur et al. | Improvement of protocols for the screening of biological control agents against white-nose syndrome |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20141210 |
|
| RJ01 | Rejection of invention patent application after publication |