CN104178446B - Application of acetylcholine and analogs thereof in promoting microalgae growth and microalgae grease accumulation - Google Patents
Application of acetylcholine and analogs thereof in promoting microalgae growth and microalgae grease accumulation Download PDFInfo
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- CN104178446B CN104178446B CN201410421791.5A CN201410421791A CN104178446B CN 104178446 B CN104178446 B CN 104178446B CN 201410421791 A CN201410421791 A CN 201410421791A CN 104178446 B CN104178446 B CN 104178446B
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Abstract
The invention discloses a method for adding neurotransmitter acetylcholine or acetylcholine analogs in a microalgae growth process to enhance microalgae biomass quantity and grease content, belonging to the field of microalgae biology. Different concentrations of neurotransmitter acetylcholine or acetylcholine analogs in different microalgae growth periods are added to analyze the influence of different concentrations of acetylcholine or acetylcholine analogs added in different periods on the Chlorella biomass quantity and grease content. The invention provides the addition of the neurotransmitter acetylcholine or acetylcholine analogs in the microalgae growth process for the first time, and provides a new idea for synchronously promoting microalgae growth and grease accumulation by using a neurotransmitter.
Description
Technical field
The invention belongs to technical field of microalga biology is and in particular to a kind of acetylcholine and the like is promoting microalgae life
Application in length and microalgae grease accumulation.
Background technology
Microalgae abundant species, can mass propgation and be not take up plough；Growth cycle is short, about 3-5 days doubling time, unit plane
Long-pending yield is tens times or even hundreds of times of grain；Photosynthetic efficiency is high, can effectively utilize solar energy, inorganic matters are turned
Turn to the nutritional labelings such as protein, polysaccharide, polyunsaturated fatty acid, can be used for food, medicine and energy aspect；Fat content
Height, reaches the 20%-70% of dry weight, far above land plant, is that one kind has promising biological raw material very much.Currently with culture
Microalgae is accumulating oils and fatss to produce biodiesel, it has also become using solar energy development Renewable resource research field the most popular,
There is powerful market potential and outstanding social value.
Compared with high oil crop, the biochemical composition of microalgae is subject to the shadow that culture salt composition and environmental condition change
Sound is larger, and such as nitrogen lacks the accumulation easily stimulating the oils and fatss especially triacylglycerol of microalgae, and temperature reduction can promote more than microalgae not
Accumulation of satisfied fatty acid etc. is it is therefore desirable to further investigate the optimum oil-producing condition of culture of microalgae.
Culture salt composition and environmental condition are more on the research of microalgae grease accumulation impact, and phytohormone isoreactivity molecule
Chemical Regulation affect microalgae grease accumulation research less, from the result of study to the impact that higher plant grows for the phytohormone
In it can be seen that phytohormone to the growth of higher plant, there is very strong adjustment effect, therefore study its shadow to micro algae growth
Ring, the amount of biomass and oil and fat accumulation speed improving microalgae is had great importance.
Microalgae autotrophy system Biomass accumulation compared with heterotrophism system is relatively low, improves biology therefore, it is possible to remarkably promote growth
The measure of matter accumulation plays an important role to setting up the microalgae autotrophy system with low-cost advantage.In prior art, microalgae is certainly
In foster system, commonly used two-step method produces oils and fatss, reaches fast-growth to form biomass under the optimal conditions of first stage
And to accumulate oils and fatss under subsequent stress conditions.Under fast-growth, oil and fat accumulation is few, and under stress conditions, Biomass accumulation is suppressed,
Integrate, final grease yield is low, reduce the practical value that microalgae autotrophy system produces oils and fatss.Therefore, invention energy
The one-step method method making synchronous accumulation oils and fatss while microalgae fast-growth is to the efficient microalgae autotrophy system setting up low cost
Produce oils and fatss technology to play a key effect.
Content of the invention
The technical problem to be solved is to provide one kind to promote micro algae growth using acetylcholine and the like
And the method that oils and fatss synchronously accumulate.
For solving above technical problem, the present invention adopts the following technical scheme that
Acetylcholine and the like promotes applying the present invention's in micro algae growth and microalgae grease accumulation at the same time
Within protection domain.
Wherein, described microalgae be chlorella, spirulina, chlamydomonas, Nannochloropsis oceanica, Haematococcus Pluvialis, the new chlorella of rich oil, multinuclear green
Algae (deasonia multinucleata), multiform scenedesmus (desmodesmus multiformis), palm net algae
Any one in (palmodictyon varium) and Wild Vitis species.
Wherein, add the analog of acetylcholine or acetylcholine in the culture medium of microalgae, the time of interpolation is microalgae
Growth phase of adjustment or logarithmic (log) phase.Preferably in microalgae od680Be worth for 0～0.5 when (phase of adjustment) add acetylcholine or acetylcholine
Analog, most preferably in microalgae od680Be worth for 0 when (phase of adjustment) add acetylcholine or acetylcholine analog；Or preferably exist
Microalgae od680It is worth and adds acetylcholine or acetylcholine analog for (logarithmic (log) phase) when 1.2～1.6, most preferably in microalgae od680Value
Add acetylcholine or acetylcholine analog for (logarithmic (log) phase) when 1.5.
Wherein, acetylcholine micro algae growth phase of adjustment addition be 0.1～5 μ g/l, preferably 0.5～5 μ g/l,
Most preferably 5 μ g/l.
Wherein, the addition of the logarithmic (log) phase in micro algae growth for the acetylcholine is 0.1～5 μ g/l, preferably 0.25～5 μ g/
L, most preferably 5 μ g/l.
Wherein, acetylcholine analog micro algae growth phase of adjustment addition be 50～200 μ g/l, preferably 150
～200 μ g/l, most preferably 200 μ g/l.
Wherein, the addition of the logarithmic (log) phase in micro algae growth for the acetylcholine analog is 50～200 μ g/l, preferably 150
～200 μ g/l, most preferably 200 μ g/l.
Wherein, the analog of described acetylcholine is choline, choline chloride, bursine, citicoline or phospholipid
Phatidylcholine, preferably choline.
Wherein, microdisk electrode temperature is 25～30 DEG C, preferably 30 DEG C；Culture intensity of illumination is 2000～4000lux, preferably
For 3000lux.
Acetylcholine and the like applying within protection scope of the present invention in promoting microalgae grease accumulation.
Wherein, described microalgae be chlorella, spirulina, chlamydomonas, Nannochloropsis oceanica, Haematococcus Pluvialis, the new chlorella of rich oil, multinuclear green
Any one in algae, multiform scenedesmus, palm net algae and Wild Vitis species.
Wherein, add the analog of acetylcholine or acetylcholine in the culture medium of microalgae, the time of interpolation is microalgae
Growth phase of adjustment or logarithmic (log) phase.Preferably in microalgae od680Be worth for 0～0.5 when (phase of adjustment) add acetylcholine or acetylcholine
Analog, most preferably in microalgae od680Be worth for 0 when (phase of adjustment) add acetylcholine or acetylcholine analog；Or preferably exist
Microalgae od680It is worth and adds acetylcholine or acetylcholine analog for (logarithmic (log) phase) when 1.2～1.6, most preferably in microalgae od680Value
Add acetylcholine or acetylcholine analog for (logarithmic (log) phase) when 1.5.
Wherein, acetylcholine micro algae growth phase of adjustment addition be 0.1～5 μ g/l, preferably 0.5～5 μ g/l,
Most preferably 5 μ g/l.
Wherein, the addition of the logarithmic (log) phase in micro algae growth for the acetylcholine is 0.1～5 μ g/l, preferably 0.25～5 μ g/
L, most preferably 5 μ g/l.
Wherein, acetylcholine analog micro algae growth phase of adjustment addition be 50～200 μ g/l, preferably 150
～200 μ g/l, most preferably 200 μ g/l.
Wherein, the addition of the logarithmic (log) phase in micro algae growth for the acetylcholine analog is 50～200 μ g/l, preferably 150
～200 μ g/l, most preferably 200 μ g/l.
Wherein, described acetylcholine analog is choline, choline chloride, bursine, citicoline or phosphatidyl
Choline, preferably choline.
Wherein, microdisk electrode temperature is 25～30 DEG C, preferably 30 DEG C；Culture intensity of illumination is 2000～4000lux, preferably
For 3000lux.
Acetylcholine and the like promotes the application in micro algae growth also within protection scope of the present invention.
Wherein, described microalgae be chlorella, spirulina, chlamydomonas, Nannochloropsis oceanica, Haematococcus Pluvialis, the new chlorella of rich oil, multinuclear green
Any one in algae, multiform scenedesmus, palm net algae and Wild Vitis species.
Wherein, add the analog of acetylcholine or acetylcholine in the culture medium of microalgae, the time of interpolation is microalgae
Growth phase of adjustment or logarithmic (log) phase.Preferably in microalgae od680Be worth for 0～0.5 when (phase of adjustment) add acetylcholine or acetylcholine
Analog, most preferably in microalgae od680Be worth for 0 when (phase of adjustment) add acetylcholine or acetylcholine analog；Or preferably exist
Microalgae od680It is worth and adds acetylcholine or acetylcholine analog for (logarithmic (log) phase) when 1.2～1.6, most preferably in microalgae od680Value
Add acetylcholine or acetylcholine analog for (logarithmic (log) phase) when 1.5.
Wherein, acetylcholine micro algae growth phase of adjustment addition be 0.1～10 μ g/l, preferably 0.5～10 μ g/
L, preferably 5 μ g/l.
Wherein, the addition of the logarithmic (log) phase in micro algae growth for the acetylcholine is 0.1～10 μ g/l, preferably 0.25～10 μ
G/l, most preferably 10 μ g/l.
Wherein, acetylcholine analog micro algae growth phase of adjustment addition be 50～200 μ g/l, preferably 150
～200 μ g/l, most preferably 200 μ g/l.
Wherein, the addition of the logarithmic (log) phase in micro algae growth for the acetylcholine analog is 50～200 μ g/l, preferably 150
～200 μ g/l, most preferably 200 μ g/l.
Wherein, described acetylcholine analog includes choline, choline chloride, bursine, citicoline, phosphatidyl
Choline, preferably choline.
Wherein, microdisk electrode temperature is 25～30 DEG C, preferably 30 DEG C；Culture intensity of illumination is 2000～4000lux, preferably
For 3000lux.
The definition of phase of adjustment of the present invention is: when microorganism enters in a new environment, due to will adapt to this
The individual habitat conditions originally different from it, reproduction speed is very slow, maintains a relatively low level；Phase of adjustment preferred microalgae od680
It is worth in the range of 0～0.5.
The definition of logarithmic (log) phase of the present invention is: microorganism has adapted to new environment, starts a large amount of Fast-propagations, quantity
Rapid growth, in logarithm or exponential curve shape, also referred to as exponential phase；Logarithmic (log) phase preferred microalgae od680It is worth for 1.2～1.6 scopes
Interior.
The definition of stable phase of the present invention is: micro organism quantity has risen to certain degree, due to nutrient etc.
Consume and the accumulation of unwanted metabolic products makes its quantity can not increase in a large number again, increased numbers are substantially equal to death toll, be in dynamic
Equilibrium time period；Stable phase is preferably microalgae od680Value is in the range of 2.5～3.0.
Beneficial effect:
The present invention has following distinguishing feature and an effect:
(1) present invention firstly provides adding the analog of acetylcholine or acetylcholine in the growth course of microalgae,
It is to promote microalgae biomass and oil and fat accumulation to provide one using the analog of neurotransmitter acetylcholine or acetylcholine
Bar new approaches.
(2) pass through the analog of supplement trace acetylcholine or acetylcholine, microalgae biomass can be obviously promoted
Accumulation and oils and fatss accumulation.
(3) producing algae oil using microalgae in prior art will be divided into Biomass accumulation and the lower algae oil of stress to accumulate two ranks
Section, micro algae growth is slow, accumulation oils and fatss efficiency is low, the present invention propose to the different times of micro algae growth add acetylcholine or
The analog of acetylcholine, is added to the growth not only having remarkably promoted microalgae in culture fluid but also synchronous promotion microalgae efficient accumulation oil
Fat it is achieved that process mode and its efficiency improving micro algae growth and oil and fat accumulation with a kind of molecule.
Brief description
Fig. 1 micro algae growth phase of adjustment, add variable concentrations acetylcholine, the change of chlorella amount of biomass.
Wherein, abscissa is incubation time, and vertical coordinate is the amount of biomass of chlorella, with extinction under 680nm wavelength for the frustule
Spend and to represent,Represent and be added without acetylcholine,The concentration of expression acetylcholine is 0.5 μ g/l,Represent second
The concentration of phatidylcholine is 5 μ g/l,The concentration of expression acetylcholine is 10 μ g/l.Data is the average of 3 parallel samplings
Value.
Fig. 2 is in the logarithmic (log) phase of micro algae growth, i.e. od680=1.5, add the acetylcholine of variable concentrations, bead algal biomass
The change of quantity.Wherein, abscissa is incubation time, and vertical coordinate is the amount of biomass of chlorella, with frustule in 680nm ripple
Absorbance under long representing,Represent and be added without acetylcholine,The concentration of expression acetylcholine is 0.5 μ g/l,The concentration of expression acetylcholine is 5 μ g/l,The concentration of expression acetylcholine is 10 μ g/l.Data is parallel to take for 3 times
The meansigma methodss of sample.
Chlorella of the same race does not add acetylcholine and is not added with acetylcholine dry weight and compares Fig. 3.Adjustment in micro algae growth
Phase, add the acetylcholine of 0.5 μ g/l, the change of different chlorella amount of biomass.Wherein, abscissa is different chlorella,
Be successively c.sorokiniana be Root Rot of Wheat chlorella, c.kessleri be Kessler chlorella, c.vulgaris is common
Chlorella, c.protothecoides is original ball chlorella；Vertical coordinate is the amount of biomass change of chlorella, and data is 3 times
The meansigma methodss of parallel sampling.
Chlorella of the same race does not add acetylcholine and is not added with acetylcholine oil and fat accumulation and compares Fig. 4.In micro algae growth
Phase of adjustment, add the acetylcholine of 0.5 μ g/l, the change of oil and fat accumulation in chlorella.Wherein, abscissa is different chlorella,
Be successively c.sorokiniana be Root Rot of Wheat chlorella, c.kessleri be Kessler chlorella, c.vulgaris is common
Chlorella, c.protothecoides is original ball chlorella；Vertical coordinate is the oil and fat accumulation change of chlorella, and data is to put down for 3 times
The meansigma methodss of row sampling.
Comparison on the impact of chlorella amount of biomass when Fig. 5 acetylcholine analog adds phase of adjustment chlorella.?
Micro algae growth phase of adjustment, be separately added into the acetylcholine analog of 50 μ g/l, 150 μ g/l and 200 μ g/l, different chlorellas lifes
The change of amount of material.Wherein, abscissa is blank and different acetylcholine analog, be successively chol be choline, ch
For choline chloride, pc is bursine, and inn is citicoline, and chcl is phosphatidylcholine；Vertical coordinate is the biology of chlorella
Matter number change, data is the meansigma methodss of 3 parallel samplings.
Comparison on the impact of chlorella oil and fat accumulation when Fig. 6 acetylcholine analog adds phase of adjustment chlorella.Micro-
Algae growth phase of adjustment, be separately added into the acetylcholine analog of 50 μ g/l, 150 μ g/l and 200 μ g/l, different chlorella oils and fatss
The change of accumulation.Wherein, abscissa is blank and different acetylcholine analog, be successively chol be choline, ch is chlorine
Change choline, pc is bursine, inn is citicoline, chcl is phosphatidylcholine；Vertical coordinate is chlorella oil and fat accumulation
Change, data is the meansigma methodss of 3 parallel samplings.
Specific embodiment
According to following embodiments, the present invention may be better understood.However, it is as it will be easily appreciated by one skilled in the art that real
Apply the content described by example and be merely to illustrate the present invention, and should not be also without limitation on basis described in detail in claims
Invention.
In following examples, microalgae is cultivated in accordance with the following methods: fresh microalgae cell is inoculated according to 5% volume fraction
To in the 250ml triangular flask of the basic tap culture medium fresh equipped with 100ml, quiescent culture, and the different times in micro algae growth
Add acetylcholine, therebetween, be shaken every day triangular flask 3～5 times, harvest when microalgae cell breeds the stable phase to maximal density
Microalgae cell, algae solution centrifugal concentrating, it is washed with deionized, is centrifuged, remove microalgae cell culture salt, be prepared into and no cultivate salt
Wet algae, is used as oil and grease extracting.
In following examples, basic tap culture medium (every liter) formula used is as follows: 400.00mg nh4Cl,
50.00mgcacl2·2h2O, 100.00mg mgso4·7h2O, 98.80mgna2hpo4, 61.73mg kh2po4,
50.00mgna2edta·2h2O, 22.00mg znso4·7h2O, 11.40mg h3bo3, 5.10mg mncl2·4h2O,
5.00mgfeso4·7h2O, 1.60mg cocl2·6h2O, 1.16mg cuso4·5h2O, 1.10mg (nh4)6mo7o24·4h2O,
2420.00mg trisma base, 1ml glacial acetic.
In following examples, the oils and fatss of microalgae obtain by the following method: add 5ml dense in the 1g microalgae dry product of gained
Hydrochloric acid (mass concentration 36%-38%), mixes, and places 20min in 70 DEG C of water-baths, adds 5ml dehydrated alcohol, after standing cooling again
Add 5ml ether in system, vibrate 1min, 4000rpm is centrifuged 2min, obtain upper strata ether phase and lower sediment, by upper strata
Ether phase removes, and adds the ether of 5ml in lower sediment, vibrates 1min, and 4000rpm is centrifuged 2min, then by upper strata ether phase
Removal, merges all upper stratas ether phase, concentrating under reduced pressure desolvation, obtains algae oil.Microalgae cell fat content is expressed as mg
g-1Dw, i.e. microalgae grease content (%)=oils and fatss weight/microalgae dry product weight * 100.
In following examples, the weight of microalgae dry product refers to the microalgae algae silty amount for carrying oil, is existed by microalgae cell
Absorbance under 680nm may determine that the upgrowth situation of microalgae, treats that micro algae growth, to stable phase, frustule is shaken up, takes 10ml
Algae solution 8000rpm is centrifuged 3min, removes culture fluid, the algae being obtained mud is dried at 70 DEG C 12h, obtains microalgae dry product, obtains
To in every ml algae solution, microalgae dry product quality, is expressed as mg/ml, that is, be used in microalgae dry product quality (the mg)=every ml algae solution carry oil
Microalgae dry product quality * is used for putting forward the microalgae algae solution volume of oil.
Embodiment 1:
In order to investigate the impact to chlorella growth and oil and fat accumulation for the acetylcholine, we configuration defined medium be
In basic tap culture medium add variable concentrations acetylcholine, control culture medium in acetylcholine concentration be 0 μ g/l, 0.125
μg/l、0.25μg/l、0.5μg/l、1μg/l、5μg/l、10μg/l.
Respectively with the chlorella that the inoculum concentration inoculation of volume fraction 5% is fresh in above-mentioned each group culture fluid, at 25 DEG C,
Cultivate to stable phase in the constant temperature illumination box of 3000lux, obtain algae solution.In incubation, take algae solution every 24h, in
Measure the absorbance of algae solution under 680nm, obtain the growth curve of chlorella.
Above-mentioned gained algae solution is shaken up, takes 10ml algae solution 8000rpm centrifugation 3min, remove culture fluid, by the algae being obtained
Mud is dried 12h at 70 DEG C, obtains microalgae dry product, obtains microalgae dry product quality in every ml algae solution, be expressed as mg/ml, that is, be used for
Put forward microalgae dry product quality * in microalgae dry product quality (mg)=every ml algae solution of oil to be used for putting forward the microalgae algae solution volume of oil.
Chlorella growth phase of adjustment, adds the impact to its amount of biomass and fat content for the variable concentrations acetylcholine to see
Shown in Tables 1 and 2.Chlorella growth phase of adjustment, adds the impact to its amount of biomass for the variable concentrations acetylcholine to see Fig. 1.Knot
Fruit shows with respect to matched group (concentration of acetylcholine is 0 μ g/l), in the phase of adjustment addition acetylcholine of chlorella growth
Enough significantly improve amount of biomass and the fat content of chlorella, the raising degree of bead algal biomass and fat content and acetyl
The concentration of choline has very big relation, and when acetylcholine concentration is 5 μ g/l, the amount of biomass of chlorella and fat content all reach
To highest, respectively the 1.26 of matched group times and 1.42 times.
Table 1 chlorella growth phase of adjustment, adds the impact to its amount of biomass for the variable concentrations acetylcholine
Table 2 chlorella growth phase of adjustment, adds the impact to its fat content for the variable concentrations acetylcholine
Embodiment 2:
Tested with the method similar to example 1, except Initial stage of culture is added without, acetylcholine, cultivate to microalgae life
The long logarithm initial stage, i.e. od680It is worth for 1.5, adding concentration is 0 μ g/l, 0.125 μ g/l, 0.25 μ g/l, 0.5 μ g/l, 1 μ g/
Outside l, 5 μ g/l, the acetylcholine of 10 μ g/l, other specification is constant.
Chlorella growth logarithmic (log) phase adds the impact to its amount of biomass and fat content for the variable concentrations acetylcholine to see
Shown in table 3 and table 4.Chlorella growth logarithmic (log) phase adds the impact to its amount of biomass for the variable concentrations acetylcholine to see Fig. 2.Knot
Fruit display, with respect to matched group (concentration of acetylcholine is 0 μ g/l), adds acetylcholine in the logarithmic (log) phase of chlorella growth
Enough significantly improve amount of biomass and the fat content of chlorella, and increased amplitude be higher than chlorella growth phase of adjustment add
Enter variable concentrations acetylcholine.The raising degree of chlorella amount of biomass and fat content and the concentration of acetylcholine have very big
Relation, with the increase of acetylcholine concentration, the amount of biomass of chlorella assumes the trend being gradually increased, but high
The acetylcholine of concentration but can lead to the decline of chlorella fat content, considers acetylcholine to chlorella amount of biomass
With the impact of fat content, select acetylcholine concentration 5 μ g/l, as the optimum concentration of chlorella growth and oil and fat accumulation,
Now the amount of biomass of chlorella and fat content are respectively 1.34 times and 1.45 times of matched group.
Table 3 chlorella growth logarithmic (log) phase adds the impact to its amount of biomass for the variable concentrations acetylcholine
Table 4 chlorella growth logarithmic (log) phase adds the impact to its fat content for the variable concentrations acetylcholine
In chlorella not of the same race: growth phase of adjustment add 5 μ g/l, as shown in Figure 3, Figure 4, result shows difference to result
Planting chlorella has obvious increasing than the amount of biomass and oil and fat accumulation being not added with acetylcholine after adding acetylcholine respectively
Plus.
Embodiment 3:
The method similar to example 1 is tested, and except Initial stage of culture is added without neurotransmitter acetylcholine, cultivates to micro-
It is 0 μ g/l, 0.125 μ g/l, 0.25 μ g/l, 0.5 μ g/l, 1 μ g/l, 5 μ g/l, 10 μ g/l that the stable phase of algae growth adds concentration
Acetylcholine outside, other specification is constant.
Chlorella growth logarithmic (log) phase adds the impact to its amount of biomass and fat content for the variable concentrations acetylcholine to see
Shown in table 5 and table 6.Result shows with respect to matched group (concentration of acetylcholine is 0 μ g/l), in the stable phase of chlorella growth
Add acetylcholine little on the amount of biomass of chlorella and the raising impact of fat content.
Table 5 chlorella growth stable phase adds the impact to its amount of biomass for the variable concentrations acetylcholine
Table 6 chlorella growth stable phase adds the impact to its fat content for the variable concentrations acetylcholine
Embodiment 4:
Tested with the method similar to example 1, except that the object of experiment is changed to spirulina, chlamydomonas, micro- green
The new chlorella of ball algae, Haematococcus Pluvialis, rich oil, multinuclear chlorella, multiform scenedesmus, palm net algae and Wild Vitis species, respectively in above-mentioned algal grown
Logarithmic (log) phase, od680It is worth for 1.5, add the acetylcholine of final concentration of 5 μ g/l in its culture, other specification is constant.
As shown in table 7～9, as can be seen from the table, adding acetylcholine in above-mentioned algae can promote experimental result
Micro algae growth and the accumulation of microalgae grease.
Table 7 acetylcholine promotes the accumulation of different algal species fatty acid
Table 8 acetylcholine promotes the accumulation of different algal species amount of biomass
Table 9 acetylcholine promotes the rate of increase of different algal species amount of biomass and oil and fat accumulation
Embodiment 5:
Tested with the method similar to example 1, except that chlorella growth phase of adjustment to different realities
Test to add in group and add 50 μ g/l, 150 μ g/l, 200 μ g/l choline, choline chloride, hydrogenation choline, citicoline or phosphatidyl
Choline, result is as shown in Figure 5, Figure 6.Wherein chol represents choline, and ch represents hydrogenation choline, and pc represents phosphatidylcholine, inn generation
Table citicoline, chcl represents choline chloride.It can be seen that adding choline precursor and the like in chlorella,
The accumulation of micro algae growth microalgae grease accumulation all can be promoted.
Claims (9)
1. acetylcholine and the like promotes the application in micro algae growth and microalgae grease accumulation at the same time；
Wherein, described microalgae be chlorella, spirulina, chlamydomonas, Nannochloropsis oceanica, Haematococcus Pluvialis, the new chlorella of rich oil, multinuclear chlorella,
Any one in multiform scenedesmus, palm net algae and Wild Vitis species；
The analog of described acetylcholine is choline, choline chloride, bursine, citicoline or phosphatidylcholine.
2. according to claim 1 application it is characterised in that micro algae growth phase of adjustment, logarithmic (log) phase to culture medium in
Add acetylcholine or acetylcholine analog；Acetylcholine is 0.1～5 μ g/l in the phase of adjustment addition of micro algae growth, second
Phatidylcholine is 0.1～5 μ g/l in the logarithmic (log) phase addition of micro algae growth；Acetylcholine analog micro algae growth phase of adjustment add
Dosage is 50～200 μ g/l, and acetylcholine analog is 50～200 μ g/l in the logarithmic (log) phase addition of micro algae growth.
3. application according to claim 1, it is characterised in that microdisk electrode temperature is 25～30 DEG C, cultivates intensity of illumination
For 2000～4000lux.
4. application in promoting microalgae grease accumulation for the acetylcholine and the like；
Wherein, described microalgae be chlorella, spirulina, chlamydomonas, Nannochloropsis oceanica, Haematococcus Pluvialis, the new chlorella of rich oil, multinuclear chlorella,
Any one in multiform scenedesmus, palm net algae and Wild Vitis species；
Described acetylcholine analog is choline, choline chloride, bursine, citicoline or phosphatidylcholine.
5. according to claim 4 application it is characterised in that micro algae growth phase of adjustment, logarithmic (log) phase to culture medium in
Add acetylcholine or acetylcholine analog；Acetylcholine is 0.1～5 μ g/l in the phase of adjustment addition of micro algae growth, second
Phatidylcholine is 0.1～5 μ g/l in the logarithmic (log) phase addition of micro algae growth；Acetylcholine analog micro algae growth phase of adjustment add
Dosage is 50～200 μ g/l, and acetylcholine analog is 50～200 μ g/l in the logarithmic (log) phase addition of micro algae growth.
6. application according to claim 4, it is characterised in that microdisk electrode temperature is 25～30 DEG C, cultivates intensity of illumination
For 2000～4000lux.
7. application in promoting micro algae growth for the acetylcholine and the like；
Wherein, described microalgae is chlorella, spirulina, chlamydomonas, Nannochloropsis oceanica, Haematococcus Pluvialis, the new chlorella of rich oil, multinuclear for microalgae
Any one in chlorella, multiform scenedesmus, palm net algae and Wild Vitis species；
Wherein, described acetylcholine analog is choline, choline chloride, bursine, citicoline or phosphatidylcholine.
8. according to claim 7 application it is characterised in that micro algae growth phase of adjustment, logarithmic (log) phase to culture medium in
Add acetylcholine or acetylcholine analog；Acetylcholine is 0.1～10 μ g/l in the phase of adjustment addition of micro algae growth, second
Phatidylcholine is 0.1～10 μ g/l in the logarithmic (log) phase addition of micro algae growth；Acetylcholine analog micro algae growth phase of adjustment
Addition is 50～200 μ g/l, and acetylcholine analog is 50～200 μ g/l in the logarithmic (log) phase addition of micro algae growth.
9. application according to claim 7, it is characterised in that microdisk electrode temperature is 25～30 DEG C, cultivates intensity of illumination
For 2000～4000lux.
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| CN201410421791.5A CN104178446B (en) | 2014-08-25 | 2014-08-25 | Application of acetylcholine and analogs thereof in promoting microalgae growth and microalgae grease accumulation |
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Non-Patent Citations (3)
| Title |
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| Evolutional study on acetylcholine expression;Yoko Horiuchi et al.;《Life Sciences》;20031231;全文 * |
| Evolutionary Considerations of Neurotransmitters in Microbial, Plant, and Animal Cells;Victoria V. Roshchina;《Microbial Endocrinology》;20101231;全文 * |
| Phenotypic Screening with Oleaginous Microalgae Reveals Modulators of Lipid Productivity;Annaliese K. Franz et al.;《ACS Chem. Biol.》;20130322;全文 * |
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