CN112391293A - Method for preparing squalene by autotrophic culture of botryococcus through heterotrophic biomembrane adherence - Google Patents
Method for preparing squalene by autotrophic culture of botryococcus through heterotrophic biomembrane adherence Download PDFInfo
- Publication number
- CN112391293A CN112391293A CN202011338162.8A CN202011338162A CN112391293A CN 112391293 A CN112391293 A CN 112391293A CN 202011338162 A CN202011338162 A CN 202011338162A CN 112391293 A CN112391293 A CN 112391293A
- Authority
- CN
- China
- Prior art keywords
- botryococcus
- squalene
- culture
- medium
- improving
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- YYGNTYWPHWGJRM-UHFFFAOYSA-N (6E,10E,14E,18E)-2,6,10,15,19,23-hexamethyltetracosa-2,6,10,14,18,22-hexaene Chemical compound CC(C)=CCCC(C)=CCCC(C)=CCCC=C(C)CCC=C(C)CCC=C(C)C YYGNTYWPHWGJRM-UHFFFAOYSA-N 0.000 title claims abstract description 54
- 241001536324 Botryococcus Species 0.000 title claims abstract description 54
- BHEOSNUKNHRBNM-UHFFFAOYSA-N Tetramethylsqualene Natural products CC(=C)C(C)CCC(=C)C(C)CCC(C)=CCCC=C(C)CCC(C)C(=C)CCC(C)C(C)=C BHEOSNUKNHRBNM-UHFFFAOYSA-N 0.000 title claims abstract description 54
- PRAKJMSDJKAYCZ-UHFFFAOYSA-N dodecahydrosqualene Natural products CC(C)CCCC(C)CCCC(C)CCCCC(C)CCCC(C)CCCC(C)C PRAKJMSDJKAYCZ-UHFFFAOYSA-N 0.000 title claims abstract description 54
- 229940031439 squalene Drugs 0.000 title claims abstract description 54
- TUHBEKDERLKLEC-UHFFFAOYSA-N squalene Natural products CC(=CCCC(=CCCC(=CCCC=C(/C)CCC=C(/C)CC=C(C)C)C)C)C TUHBEKDERLKLEC-UHFFFAOYSA-N 0.000 title claims abstract description 54
- 238000000034 method Methods 0.000 title claims abstract description 25
- 230000001651 autotrophic effect Effects 0.000 title description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 16
- 239000001963 growth medium Substances 0.000 claims abstract description 11
- 239000008103 glucose Substances 0.000 claims abstract description 10
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 9
- 238000012258 culturing Methods 0.000 claims abstract description 9
- 238000004519 manufacturing process Methods 0.000 claims abstract description 8
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 8
- 239000002609 medium Substances 0.000 claims description 14
- 238000006243 chemical reaction Methods 0.000 claims description 4
- 239000000835 fiber Substances 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 239000011148 porous material Substances 0.000 claims description 3
- 230000001464 adherent effect Effects 0.000 claims 1
- 241000195493 Cryptophyta Species 0.000 abstract description 6
- 230000015572 biosynthetic process Effects 0.000 abstract description 5
- 241000251730 Chondrichthyes Species 0.000 abstract description 3
- 238000003786 synthesis reaction Methods 0.000 abstract description 3
- 241001290352 Caulerpa racemosa Species 0.000 abstract description 2
- 230000007547 defect Effects 0.000 abstract description 2
- 230000007613 environmental effect Effects 0.000 abstract description 2
- 230000001902 propagating effect Effects 0.000 abstract 1
- 238000004115 adherent culture Methods 0.000 description 8
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 6
- 229910052799 carbon Inorganic materials 0.000 description 6
- 238000009630 liquid culture Methods 0.000 description 6
- 229930195733 hydrocarbon Natural products 0.000 description 5
- 238000005286 illumination Methods 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 239000004215 Carbon black (E152) Substances 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 150000002430 hydrocarbons Chemical class 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000002028 Biomass Substances 0.000 description 2
- 241001536303 Botryococcus braunii Species 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000006194 liquid suspension Substances 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 2
- 238000004114 suspension culture Methods 0.000 description 2
- -1 triterpenoid hydrocarbon Chemical class 0.000 description 2
- HMUNWXXNJPVALC-UHFFFAOYSA-N 1-[4-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]piperazin-1-yl]-2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethanone Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)N1CCN(CC1)C(CN1CC2=C(CC1)NN=N2)=O HMUNWXXNJPVALC-UHFFFAOYSA-N 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 206010035664 Pneumonia Diseases 0.000 description 1
- GZCGUPFRVQAUEE-SLPGGIOYSA-N aldehydo-D-glucose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O GZCGUPFRVQAUEE-SLPGGIOYSA-N 0.000 description 1
- FRHBOQMZUOWXQL-UHFFFAOYSA-L ammonium ferric citrate Chemical compound [NH4+].[Fe+3].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O FRHBOQMZUOWXQL-UHFFFAOYSA-L 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 238000003501 co-culture Methods 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 235000015872 dietary supplement Nutrition 0.000 description 1
- XQGPKZUNMMFTAL-UHFFFAOYSA-L dipotassium;hydrogen phosphate;trihydrate Chemical compound O.O.O.[K+].[K+].OP([O-])([O-])=O XQGPKZUNMMFTAL-UHFFFAOYSA-L 0.000 description 1
- 229960001484 edetic acid Drugs 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 229960004642 ferric ammonium citrate Drugs 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 239000004313 iron ammonium citrate Substances 0.000 description 1
- 235000000011 iron ammonium citrate Nutrition 0.000 description 1
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 description 1
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000004323 potassium nitrate Substances 0.000 description 1
- 235000010333 potassium nitrate Nutrition 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
- C12P5/026—Unsaturated compounds, i.e. alkenes, alkynes or allenes
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a method for preparing squalene by culturing Botryococcus, which improves the yield of squalene in the culture process of Botryococcus by optimizing the culture mode of the Botryococcus for producing squalene; further improving the medical application of the grape alga squalene, reducing the environmental damage caused by the application and development of the squalene and making up the defects of the prior art. The invention firstly provides a method for culturing Botryococcus, which is characterized in that glucose is added into BG11 culture medium to culture Botryococcus, thereby propagating algae. The invention also provides a method for improving the squalene production of Botryococcus, which is to culture the Botryococcus in the BG11 culture medium and reduce the nitrogen concentration in the BG11 culture medium. The invention promotes the synthesis of squalene under the condition of not influencing the growth of botryococcus, thereby improving the yield of squalene with medical value, improving the application prospect of botryococcus squalene products, and hopefully reducing the killing amount of sharks, thereby protecting marine ecological environment.
Description
Technical Field
The invention belongs to the technical field of botryococcus propagation culture and high-value product production, and particularly relates to a method for preparing squalene by culturing botryococcus.
Background
Squalene (Squalene) is used as a biosynthesis precursor of plant and animal steroids, is unsaponifiable colorless oily triterpenoid hydrocarbon, has remarkable antioxidant and antibacterial activities, and is widely applied to industries such as biomedicine, food supplements, high-quality cosmetics and the like. In 2020, some new coronary pneumonia candidate vaccines contain squalene as reported in the uk medium. Only 2014, the global squalene market demand reaches 26 million tons, and the market value reaches 2.4 hundred million dollars.
However, the current commercial source of squalene is primarily liver oil from deep sea sharks, but the continuous supply and future availability of animal liver oil will be greatly limited due to the conservation of marine wildlife and fishery resources. Therefore, the search for a new resource of squalene that can replace animal sources is an urgent task to meet the commercial production of high-quality squalene and to improve marine ecology. Research shows that some plants, microorganisms and a few microalgae in the nature have the capacity of synthesizing squalene under a controlled environment, but the generated squalene product is mostly gathered in a specific subcellular region to damage host cells, and the characteristic feedback inhibits the continuous biosynthesis of squalene.
The botryococcus in the microalgae can synthesize triterpene hydrocarbons rich in squalene, which account for about 30-50% of the total amount of hydrocarbons, and the squalene synthesis process does not damage algal cells, so that the botryococcus is a squalene resource substitute with application prospect. However, as the cells of the algae grow slowly, the research on the production of the botryococcus squalene is not much concerned, and the medical application of the botryococcus squalene is also greatly limited.
Disclosure of Invention
The invention aims to provide a method for preparing squalene by culturing Botryococcus, which improves the yield of squalene in the culture process of Botryococcus by optimizing the culture mode of the Botryococcus for producing squalene; further improving the medical application of the grape alga squalene, reducing the environmental damage caused by the application and development of the squalene and making up the defects of the prior art.
The invention firstly provides a method for culturing Botryococcus, which is characterized in that glucose is added into a BG11 culture medium to culture the Botryococcus, so as to propagate algae;
preferably, the adding concentration of the glucose is 2.5 g/L;
the invention also provides a method for improving the squalene production of Botryococcus, which is to culture the Botryococcus in the BG11 culture medium and reduce the nitrogen concentration in the BG11 culture medium;
preferably, the nitrogen concentration in the BG11 culture medium is reduced to one half of the background value or zero nitrogen concentration;
as a specific description of the examples, the BG11 medium was adjusted to a nitrogen concentration of one fourth of the background (0.375 g.L)-1)。
Furthermore, the method is to culture in a biomembrane adherence reaction device;
as a specific description of the embodiment, a mixed fiber filter membrane material with a pore size of 0.22 μm is used in the biofilm adherence reaction device.
The invention promotes the synthesis of squalene under the condition of not influencing the growth of botryococcus, thereby improving the yield of squalene with medical value, improving the application prospect of botryococcus squalene products, and hopefully reducing the killing amount of sharks, thereby protecting marine ecological environment.
Drawings
FIG. 1: schematic device diagrams of a botryococcus liquid culture (a) and a biofilm adherent culture (b);
FIG. 2: graph comparing the change of the growth of the algae cells (a), the hydrocarbon (b) and the squalene content (c) in the liquid culture and the adherent culture of the botryococcus;
FIG. 3: a graph of the effect of different nitrogen sources and concentrations on the yield of squalene from Botryococcus;
FIG. 4: a plot of the variation of different N concentrations on the growth of botryococcus cells (a) and squalene production (b);
FIG. 5: and (3) a graph of biological yield of Botryococcus under heterotrophic conditions and squalene content.
Detailed Description
The present invention will be described in detail below with reference to examples and the accompanying drawings.
Example 1: comparison of Botryococcus liquid culture with Squalene production by biofilm adherent culture
Culturing Botryococcus (B.braunii) in BG11 medium with 1% CO2Liquid culture and adherent culture were carried out at a concentration (volume ratio), illumination intensity of 3000lux, and temperature of 25 ℃ respectively (FIGS. 1a and b). BG11 (g.L)-1) The components of the medium were distributed as follows: 1.5 portions of potassium nitrate, 0.075 portion of magnesium sulfate heptahydrate, 0.04 portion of dipotassium phosphate trihydrate, 0.027 portion of anhydrous calcium chloride, 0.02 portion of sodium carbonate, 0.006 portion of citric acid, 0.006 portion of ferric ammonium citrate, 0.001 portion of ethylene diamine tetraacetic acid and 5mL of A5 solution, and the pH value is 7.5.
The Photobioreactor (PBR) for the B.braunii liquid suspension culture experiment consisted of a flat plate reactor (length 50cm, width 20cm, thickness 1cm, volume 1L) with an illumination area of 0.1m2(ii) a The adherent culture is to separate Botryococcus from culture medium, place the algae cells on the mixed fiber filter membrane material with pore size of 0.22 μm, and perform CO culture under certain illumination intensity2Photoautotrophic mode at concentration.
After 10 days of culture, the results are shown in FIG. 2, and the biological productivity of algal cells in adherent culture is higher than that in liquid culture, and is 5.24 g.m.-2.d-1、4.12g.m-2.d-1(ii) a The hydrocarbon content was not very different for the two culture modes (FIG. 2 b); however, the content of squalene in adherent culture was higher than in liquid culture (FIG. 2 c).
Example 2: effect of different carbon sources and their concentrations on Botryococcus growth under heterotrophic conditions
The Botryococcus culture was performed under the same illumination intensity (3000lux) and the same conditions as in example 1 at an addition concentration of 2.0g.L-1In the culture medium of different organic carbon, the organic carbon sources are glycerol, glucose and acetic acid, respectively. The culture results are shown in FIG. 3 a. KnotThe result shows that the botryococcus are best grown in the culture medium with the organic carbon source of glucose, and the biological yield is 1.01g.L-1.d-1。
Under the condition of determining that the organic carbon source is glucose, different carbon source concentrations of 0.5, 1.0, 2.5 and 5.0g.L are respectively set-1The culture is carried out. The results showed that the concentration of Botryococcus at glucose was 2.5g.L-1The growth is best, and the biological yield is 1.21g.L-1.d-1。
Example 3: adjusting N concentration to increase squalene yield of Botryococcus under autotrophic conditions
Botryococcus were cultured adherently in BG11 medium at different initial N concentrations for 7 days under the same conditions as in example 1. Initial N concentrations were set to normal BG11 (1.5 g.L), respectively-1)、1/2N(0.75g.L-1)、1/4N(0.375g.L-1)、1/8N(0.1875g.L-1)、0N。
As shown in FIG. 4, the difference between Botryococcus and normal BG11, 1/2N and 1/4N was small, and the biological yields were 5.24, 5.08 and 4.86g.m, respectively-2.d-1The growth of algae cells is inhibited under the condition that the N concentration is 1/8N and 0N, and the biological yield is 3.32 and 2.83g.m-2.d-1。
In terms of squalene content, the squalene content under low N condition is obviously improved, and the squalene content under different N concentration conditions (normal BG11, 1/2N, 1/4N, 1/8N and 0N) is respectively 0.27, 0.32, 0.41, 0.39 and 0.40mg.g-1(ii) a And 1/4N (0.375 g.L)-1) The lower angle yields squalene were highest.
Example 4: heterotrophic culture for increasing the yield of botryococcus, and autotrophic culture for increasing the yield of squalene
Culturing Botryococcus in 3L fermentation tank at the concentration of 2.5g.L-1Glucose in BG11 medium (BG 11 medium formulation as above) was cultured at 25 ℃ for 14 days. Then, the algal bodies were collected as a seed solution and inoculated into a biofilm adherent culture apparatus using a low N medium (1/4N,0.375 g.L.) as described in example 3-1) At 1% CO2Adherence is carried out under the conditions of concentration (volume ratio) and temperature of 25 DEG CCulturing for 7 days, collecting Botryococcus cells, and determining the biomass and squalene content of the Botryococcus cells. The results are shown in FIG. 5. The biomass of Botryococcus is 31.2g.L-1The biological yield is 1.48g.L-1.d-1The content of squalene is 0.28mg.g-1The yield of squalene is 0.41mg.L-1.d-1。
The result shows that compared with other reported methods, the method greatly improves the biological yield of the botryococcus and the yield of the botryococcus squalene is improved by about 40 percent compared with the traditional liquid suspension culture under the same condition, thereby providing a feasible path for the industrial production of the botryococcus squalene.
Claims (8)
1. A method for culturing Botryococcus is characterized in that glucose is added into BG11 culture medium to culture the Botryococcus.
2. The method of claim 1, wherein the glucose is added at a concentration of 2.5 g/L.
3. The method for improving squalene production of Botryococcus is characterized in that the Botryococcus is cultured in BG11 medium, and the nitrogen concentration in the BG11 medium is reduced.
4. The method as claimed in claim 3, wherein the BG11 medium is reduced by reducing the concentration of nitrogen in the BG11 medium to one-half the background value or to zero.
5. The method as claimed in claim 3 or 4, wherein the BG11 medium is reduced by a factor of four relative to the background value of the BG11 medium.
6. The method of any one of claims 3-5, wherein the method is culturing in a biofilm adherent reaction device.
7. The method of claim 6, wherein a mixed fiber filter material having a pore size of 0.22 μm is used in the biofilm adherence reaction device.
8. The method of claim 3, wherein the Botryococcus is cultured by the method of claim 1 or 2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011338162.8A CN112391293A (en) | 2020-11-25 | 2020-11-25 | Method for preparing squalene by autotrophic culture of botryococcus through heterotrophic biomembrane adherence |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011338162.8A CN112391293A (en) | 2020-11-25 | 2020-11-25 | Method for preparing squalene by autotrophic culture of botryococcus through heterotrophic biomembrane adherence |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112391293A true CN112391293A (en) | 2021-02-23 |
Family
ID=74607181
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011338162.8A Pending CN112391293A (en) | 2020-11-25 | 2020-11-25 | Method for preparing squalene by autotrophic culture of botryococcus through heterotrophic biomembrane adherence |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112391293A (en) |
Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100041120A1 (en) * | 2008-08-11 | 2010-02-18 | University Of Kentucky Research Foundation | Botryoccocus braunii Triterpene Synthase Proteins and Nucleic Acid Molecules, and Methods for Their Use |
| US20120088278A1 (en) * | 2010-10-11 | 2012-04-12 | C/O Phycoil Biotech International, Inc. | Utilization of Wastewater for Microalgal Cultivation |
| US20120171734A1 (en) * | 2009-07-01 | 2012-07-05 | The Regents Of The University Of California | Extraction of extracellular terpenoids from microalgae colonies |
| CN102656261A (en) * | 2009-09-18 | 2012-09-05 | 菲科尔生物技术国际公司 | Microalgae fermentation using controlled illumination |
| CN103642694A (en) * | 2013-12-13 | 2014-03-19 | 嘉兴泽元生物制品有限责任公司 | High-yield cultivation method of botryococcus |
| CN103773687A (en) * | 2012-10-23 | 2014-05-07 | 中国石油化工股份有限公司 | Culture method of microalgae |
| CN104178446A (en) * | 2014-08-25 | 2014-12-03 | 江苏省农业科学院 | Application of acetylcholine and analogs thereof in promoting microalgae growth and microalgae grease accumulation |
| CN104726445A (en) * | 2015-02-17 | 2015-06-24 | 中国计量学院 | Culture method for improving yield of botryococcus polysaccharides |
| US9487762B1 (en) * | 2012-10-09 | 2016-11-08 | University Of Kentucky Research Foundation | Method and system for producing triterpenes |
| CN106635808A (en) * | 2015-12-01 | 2017-05-10 | 华南理工大学 | Method for promoting rapid growth of microalgae by co-culture |
| CN106755250A (en) * | 2016-12-27 | 2017-05-31 | 山东金晶生物技术有限公司 | A kind of preservation of haematococcus pluvialis green cell and the large-scale method for producing of astaxanthin induction |
| CN107201314A (en) * | 2017-07-08 | 2017-09-26 | 东北师范大学 | It is a kind of while improving the microalgae Heterotrophic culture method of biomass and fat content |
-
2020
- 2020-11-25 CN CN202011338162.8A patent/CN112391293A/en active Pending
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100041120A1 (en) * | 2008-08-11 | 2010-02-18 | University Of Kentucky Research Foundation | Botryoccocus braunii Triterpene Synthase Proteins and Nucleic Acid Molecules, and Methods for Their Use |
| US20120171734A1 (en) * | 2009-07-01 | 2012-07-05 | The Regents Of The University Of California | Extraction of extracellular terpenoids from microalgae colonies |
| CN102656261A (en) * | 2009-09-18 | 2012-09-05 | 菲科尔生物技术国际公司 | Microalgae fermentation using controlled illumination |
| US20120088278A1 (en) * | 2010-10-11 | 2012-04-12 | C/O Phycoil Biotech International, Inc. | Utilization of Wastewater for Microalgal Cultivation |
| US9487762B1 (en) * | 2012-10-09 | 2016-11-08 | University Of Kentucky Research Foundation | Method and system for producing triterpenes |
| CN103773687A (en) * | 2012-10-23 | 2014-05-07 | 中国石油化工股份有限公司 | Culture method of microalgae |
| CN103642694A (en) * | 2013-12-13 | 2014-03-19 | 嘉兴泽元生物制品有限责任公司 | High-yield cultivation method of botryococcus |
| CN104178446A (en) * | 2014-08-25 | 2014-12-03 | 江苏省农业科学院 | Application of acetylcholine and analogs thereof in promoting microalgae growth and microalgae grease accumulation |
| CN104726445A (en) * | 2015-02-17 | 2015-06-24 | 中国计量学院 | Culture method for improving yield of botryococcus polysaccharides |
| CN106635808A (en) * | 2015-12-01 | 2017-05-10 | 华南理工大学 | Method for promoting rapid growth of microalgae by co-culture |
| CN106755250A (en) * | 2016-12-27 | 2017-05-31 | 山东金晶生物技术有限公司 | A kind of preservation of haematococcus pluvialis green cell and the large-scale method for producing of astaxanthin induction |
| CN107201314A (en) * | 2017-07-08 | 2017-09-26 | 东北师范大学 | It is a kind of while improving the microalgae Heterotrophic culture method of biomass and fat content |
Non-Patent Citations (6)
| Title |
|---|
| 孙凯峰等: "N、P 营养盐胁迫对两株布朗葡萄藻生长的影响", 《水生生物学报》 * |
| 张芳芳: "一株富含鲨烯的丛粒藻藻株鉴定、烃成分分析及缺氮对烃脂积累影响的初步研究" * |
| 张芳芳: "一株富含鲨烯的丛粒藻藻株鉴定、烃成分分析及缺氮对烃脂积累影响的初步研究", 《中国优秀博硕士学位论文全文数据库(硕士)基础科学辑》 * |
| 程鹏飞等: "葡萄藻生物膜贴壁培养处理含钴工业废水与烃类生产的耦合" * |
| 程鹏飞等: "葡萄藻生物膜贴壁培养处理含钴工业废水与烃类生产的耦合", 《环境科学》 * |
| 靳同霞等: "富营养废水中碳氮磷浓度对布朗葡萄藻总脂和总烃的影响", 《植物科学学报》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Azma et al. | Improvement of medium composition for heterotrophic cultivation of green microalgae, Tetraselmis suecica, using response surface methodology | |
| Doucha et al. | Production of high-density Chlorella culture grown in fermenters | |
| Heredia-Arroyo et al. | Oil accumulation via heterotrophic/mixotrophic Chlorella protothecoides | |
| KR101577820B1 (en) | Novel culture process for a heterotrophic microalga | |
| Çelekli et al. | Modeling of biomass production by Spirulina platensis as function of phosphate concentrations and pH regimes | |
| KR20160091905A (en) | Process for enrichment of microalgal biomass with carotenoids and with proteins | |
| MX2020006860A (en) | Reactors and submerged fermentation methods for producing microbe-based products. | |
| RU2728345C1 (en) | Methylococcus capsulatus vkpm b-13479 strain - producer of microbial protein mass, resistant to aggressive medium | |
| JP2017511134A (en) | Method for culturing Auranthiochytrium microalgae in chloride and sodium free medium for the production of DHA | |
| CN108410737A (en) | A kind of two-steps tissue culture method of purple ball algae | |
| CN103882072B (en) | A kind of method utilizing schizochytrium limacinum to produce docosahexenoic acid | |
| CN103992959A (en) | Long-chain dibasic acid producing strain and preparation method and application thereof | |
| Rismani-Yazdi et al. | High-productivity lipid production using mixed trophic state cultivation of Auxenochlorella (Chlorella) protothecoides | |
| CN103602591B (en) | A kind of schizochytrium limacinum and the method for the production of docosahexaenoic acid grease | |
| Ajijah et al. | Utilization of tofu wastewater as a cultivation medium for Chlorella vulgaris and Arthrospira platensis | |
| CN109456905B (en) | Cryptococcus rhodochrous for promoting microalgae to utilize sucrose and application thereof | |
| AU2020101953A4 (en) | A method of cultivating microalgae with high oil content | |
| Nur | Potential of palm oil mill effluent (POME) as medium growth of Chlorella sp for bioenergy production | |
| CN112391293A (en) | Method for preparing squalene by autotrophic culture of botryococcus through heterotrophic biomembrane adherence | |
| GARG et al. | Continuous production of citric acid by immobilized whole cells of Aspergillus niger | |
| KR101765833B1 (en) | Cultivation method of microalgae using bicarbonate as carbon source | |
| Imamoglu et al. | Semi-continuous cultivation of Haematococcus pluvialis for commercial production | |
| Bansal | Mixotrophic growth of Chlorella Sp. using Glycerol for the production of Biodiesel: A Review | |
| CN114196546A (en) | Application of DCMU (dendritic cell activator-responsive unit) in stabilizing microalgae polyculture growth pH (potential of hydrogen) or improving microalgae polyculture growth speed | |
| KR101670703B1 (en) | Culturing method of microalgae for increasing lipid content |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20210223 |
|
| WD01 | Invention patent application deemed withdrawn after publication |