CN104178434A - Method for preparing industrial yeast by use of antibiotic mushroom dregs - Google Patents
Method for preparing industrial yeast by use of antibiotic mushroom dregs Download PDFInfo
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- CN104178434A CN104178434A CN201410426366.5A CN201410426366A CN104178434A CN 104178434 A CN104178434 A CN 104178434A CN 201410426366 A CN201410426366 A CN 201410426366A CN 104178434 A CN104178434 A CN 104178434A
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Abstract
The invention discloses a method for preparing industrial yeast by use of antibiotic mushroom dregs. The method comprises the following steps: adding the antibiotic mushroom dregs into a fermentation tank, and configuring to form a water system; ventilating steam into the fermentation tank, sterilizing, and introducing an engineering strain into the fermentation tank so as to decompose antibiotics in the mushroom dregs and remove residues; introducing a yeast strain into the fermentation tank to carry out fermentation to obtain industrial yeast fermentation liquor; and separating and drying the industrial yeast to obtain the industrial yeast. The prepared industrial yeast can be used as a fermentation culture medium of products such as abamectin, tylosin, oxytetracycline and erythromycin or can be used for other industrial purposes. The method disclosed by the invention can be used for realizing innocent treatment and resource utilization of the antibiotic mushroom dregs, can be used for changing waste into things of value, and can be used for solving an environmental pollution problem. The method is especially suitable for innocent treatment on beta lactam antibiotic mushroom dregs such as cephalosporin C mushroom dregs and penicillin mushroom dregs.
Description
Technical field
The present invention relates to a kind of method of preparing industrial yeast, a kind of particularly method of utilizing antibiotic bacterium dregs to prepare industrial yeast, belongs to antibiotic bacterium dregs waste clean technical field.
Background technology
In antibiotic production process, follow a large amount of bacterium slags to generate, taking beta-lactam class antibiotic bacterium dregs as example, the meta-bolites producing in substratum that the main component of its bacterium slag is antibiotic bacterium filament, do not utilized, fermenting process, degradation product and a small amount of microbiotic etc. of substratum, belong to solid hazardous waste, its discharge, makes a big impact to environment.Conventional treatment process has burning disposal, Fertilizer Transformed processing, landfill disposal, fermentation and biogas production etc. at present.Burning method processing cost is high, the deficiency in economic performance of enterprise, and antibiotic bacterium dregs also can produce the multiple harmful substances such as one-level carcinogens dioxin, oxynitride while burning.Owing to being rich in organic matter, protein, Mierocrystalline cellulose and various trace elements in antibiotic bacterium dregs, therefore, can be used for making organic fertilizer, but its fertilizer in use residual microbiotic in microorganism and organism, accumulate, form resistance, exist potential ecological risk.Landfill disposal can produce secondary pollution, and wastes a large amount of land resources, and processing cost is higher., owing to still containing antibiotic bacterium dregs in the slag of natural pond, there is equally secondary pollution in bacterium slag fermentation and biogas production., how antibiotic bacterium dregs is rationally utilized for this reason, reduce environmental pollution, become the problem of this field common concern.
Summary of the invention
The object of this invention is to provide a kind of method of utilizing antibiotic bacterium dregs to prepare industrial yeast, high to solve antibiotic bacterium dregs conventional process cost, innoxious not thorough, there is secondary pollution, the problem that ecotope is worked the mischief.
The object of the present invention is achieved like this: a kind of method of utilizing antibiotic bacterium dregs to prepare industrial yeast, comprises the following steps:
(1) the bacterium slag of producing microbiotic generation is placed in to fermentor tank, adds a water to be configured to solid content
It is 1%～30% bacterium pulp water system;
(2) carry out disinfection to passing into steam in fermentor tank, sterilisation temp is 110～130 DEG C, the time 15～
60min, tank pressure is 0.10～0.2Mpa;
(3) in fermentor tank, introduce colibacillus engineering strain, bacterial strain add-on is antibiotic bacterium dregs water body
Being 1/30～1/15 of quality, is 20～40 DEG C in temperature, and pressure is 0.01～0.07MPa, and air flow quantity is 1.0～2.0L/min, and the time is under 18～36h condition, to make the microbiotic in bacterium slag decompose, to eliminate the antibiotic remains in antibiotic bacterium dregs;
(4) in fermentor tank, introduce yeast strain, yeast strain add-on is 1/30～1/15 of antibiotic bacterium dregs aqueous systems quality, it is 20～40 DEG C in temperature, pressure is 0.01～0.07MPa, air flow quantity is 0.1～0.5L/min, time is to ferment under the condition of 18～36h, obtains industrial yeast fermented liquid;
(5) industrial yeast fermented liquid step (4) being obtained prepares industrial yeast after separating, being dried.
In the present invention, antibiotic bacterium dregs refers to cephalosporin bacterium slag, penicillin mushroom dregs or other beta-lactam class antibiotic bacterium dregs.
In the present invention, colibacillus engineering strain is: bacillus coli DH 5 ɑ engineering strain or e. coli jm109 engineering strain.
Method of the present invention is applied to the processing of cephalosporin bacterium slag, penicillin mushroom dregs or other beta-lactam class antibiotic bacterium dregs especially.
In the present invention, yeast strain is: wine brewing Yeast strain of beer or candida bacterial strain or bread yeast bacterial strain.
The fermention medium that the prepared industrial yeast of the present invention can be used as the products such as Avrmectin, Desmycosin, terramycin, erythromycin with or do other industrial use.
The beneficial effect that the present invention obtains is as follows:
The present invention makes antibiotic bacterium dregs innocent processing, has solved the pollution problem of bacterium slag to environment; Make antibiotic bacterium dregs resource utilization, turn waste into wealth, reduced the wasting of resources.
Embodiment
Following examples are used for illustrating the present invention.
Embodiment 1
Utilize cephalosporin bacterium slag to prepare industrial yeast, concrete steps are as follows: cephalosporin bacterium slag is added in fermentor tank, adding water to be configured to solid content is 10% aqueous systems 100 t, then carry out disinfection to passing into steam in fermentor tank, sterilisation temp is 110～130 DEG C, time is 15～60min, and tank pressure is 0.10～0.2MPa.Sterilization finishes to introduce 5 t bacillus coli DH 5 ɑ engineering strains in backward fermentor tank, it is 40 DEG C in temperature, pressure is 0.01～0.07MPa, air flow quantity is 1.0～2.0L/min, time is to make the decomposition of cephalosporin C in bacterium slag under the condition of 18h, in elimination bacterium slag, cephalosporin is residual, realizes the innoxious of cephalosporin bacterium slag.In fermentor tank, introducing 5 t wine brewing Yeast strain of beer, is 30 DEG C in temperature, and pressure is 0.01～0.07MPa, and air flow quantity is 0.1～0.5L/min, ferments and obtain industrial yeast fermented liquid under the condition that the time is 18h; After being separated, be dried, industrial yeast fermented liquid prepares industrial yeast.The fermention medium when industrial yeast of preparation can be used as other industrial fermentation product fermentation such as Avrmectin, terramycin, erythromycin is used.
Embodiment 2
Utilize penicillin mushroom dregs to prepare industrial yeast, concrete steps are as follows: penicillin mushroom dregs is added in fermentor tank, adding water to be configured to solid content is 10% aqueous systems 100t, then carry out disinfection to passing into steam in fermentor tank, controlling temperature is 110～130 DEG C, time is 15～60min, and tank pressure is 0.10～0.2MPa.Sterilization finishes to introduce 5 t bacillus coli DH 5 ɑ engineering strains in backward fermentor tank, it is 30 DEG C in temperature, pressure is 0.01～0.07MPa, air flow quantity is 1.0～2.0L/min, time is under the condition of 36h, to make the penicillin in bacterium slag decompose, in elimination bacterium slag, penicillin is residual, realizes the innoxious of penicillin mushroom dregs.In fermentor tank, introducing 5 t candida bacterial strains, is 26 DEG C in temperature, pressure 0.01～0.07MPa, and air flow quantity is 0.1～0.5L/min, ferments and obtain industrial yeast fermented liquid under the condition that the time is 36h; Industrial yeast fermented liquid is prepared industrial yeast after separating, being dried.The fermention medium that the industrial yeast of preparation can be used as the products such as Avrmectin, Desmycosin, terramycin, erythromycin with or do other industrial use.
Embodiment 3
Embodiment 3 difference from Example 1 are that yeast strain is bread yeast bacterial strain, and other conditions are identical.
Embodiment 4
Embodiment 4 difference from Example 1 are that colibacillus engineering strain is e. coli jm109 engineering strain, and other conditions are identical.
Embodiment 5
Embodiment 5 difference from Example 2 are that colibacillus engineering strain is e. coli jm109 engineering strain, and other conditions are identical.
Embodiment 6
Embodiment 6 difference from Example 3 are that colibacillus engineering strain is e. coli jm109 engineering strain, and other conditions are identical.
Claims (4)
1. utilize antibiotic bacterium dregs to prepare a method for industrial yeast, it is characterized in that comprising the following steps:
(1) be placed in fermentor tank by producing the bacterium slag that microbiotic produces, adding water to be configured to solid content is 1%～30% bacterium pulp water system;
(2) carry out disinfection to passing into steam in fermentor tank, sterilisation temp is 110～130 DEG C, time 15～60min, and tank pressure is 0.10～0.2Mpa;
(3) in fermentor tank, introduce colibacillus engineering strain, bacterial strain add-on is 1/30～1/15 of antibiotic bacterium dregs aqueous systems quality, it is 20～40 DEG C in temperature, pressure is 0.01～0.07MPa, air flow quantity is 1.0～2.0L/min, time is under 18～36h condition, to make the microbiotic in bacterium slag decompose, and eliminates the antibiotic remains in antibiotic bacterium dregs;
(4) in fermentor tank, introduce yeast strain, yeast strain add-on is 1/30～1/15 of antibiotic bacterium dregs aqueous systems quality, it is 20～40 DEG C in temperature, pressure is 0.01～0.07MPa, air flow quantity is 0.1～0.5L/min, time is to ferment under the condition of 18～36h, obtains industrial yeast fermented liquid;
(5) industrial yeast fermented liquid step (4) being obtained prepares industrial yeast after separating, being dried.
2. method according to claim 1, is characterized in that antibiotic bacterium dregs is cephalosporin bacterium slag or penicillin mushroom dregs.
3. method according to claim 1, is characterized in that colibacillus engineering strain is: bacillus coli DH 5 ɑ bacterial strain or e. coli jm109 bacterial strain.
4. method according to claim 1, is characterized in that yeast strain is: wine brewing Yeast strain of beer or candida bacterial strain or bread yeast bacterial strain.
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| CN201410426366.5A CN104178434A (en) | 2014-08-27 | 2014-08-27 | Method for preparing industrial yeast by use of antibiotic mushroom dregs |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112337033A (en) * | 2020-11-24 | 2021-02-09 | 内蒙古科技大学 | Harmless treatment method of abamectin slag, product and application of product as sandy soil modifier |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101245320A (en) * | 2007-02-13 | 2008-08-20 | 上海来益生物药物研究开发中心有限责任公司 | Method for processing vancomycin fermentation waste slag with solid-state fermentation |
| CN103214498A (en) * | 2013-04-20 | 2013-07-24 | 河北美邦工程科技有限公司 | Penicillin fermentation broth treating technology |
| CN103230659A (en) * | 2013-03-22 | 2013-08-07 | 宋生 | Process for treatment of beta-lactam ring antibiotic filter residue |
-
2014
- 2014-08-27 CN CN201410426366.5A patent/CN104178434A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101245320A (en) * | 2007-02-13 | 2008-08-20 | 上海来益生物药物研究开发中心有限责任公司 | Method for processing vancomycin fermentation waste slag with solid-state fermentation |
| CN103230659A (en) * | 2013-03-22 | 2013-08-07 | 宋生 | Process for treatment of beta-lactam ring antibiotic filter residue |
| CN103214498A (en) * | 2013-04-20 | 2013-07-24 | 河北美邦工程科技有限公司 | Penicillin fermentation broth treating technology |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112337033A (en) * | 2020-11-24 | 2021-02-09 | 内蒙古科技大学 | Harmless treatment method of abamectin slag, product and application of product as sandy soil modifier |
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Application publication date: 20141203 |
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