CN104173393B - The extracting method of flavone component in leaf of Moringa - Google Patents
The extracting method of flavone component in leaf of Moringa Download PDFInfo
- Publication number
- CN104173393B CN104173393B CN201410366619.4A CN201410366619A CN104173393B CN 104173393 B CN104173393 B CN 104173393B CN 201410366619 A CN201410366619 A CN 201410366619A CN 104173393 B CN104173393 B CN 104173393B
- Authority
- CN
- China
- Prior art keywords
- leaf
- moringa
- extraction
- extracting method
- microwave
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Extraction Or Liquid Replacement (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides the extracting method of flavone component in leaf of Moringa, and it includes following operating procedure：Leaf of Moringa powder is taken using 50~60%v/v ethanol as solvent, solid-liquid ratio 1:50~60g/ml, microwave abstracting is carried out, collects extract solution, you can；Wherein, microwave power is 300~400W, and extraction time is 6~10min.Present invention also offers extract prepared by the extracting method.Present invention research is found, using identical leaf of Moringa powder as sample, total flavones of Moringa oleifera leaf content obtained by the method for microwave abstracting is higher than reflux extraction method, while substantially reduces extraction time, has saved the water and electricity consumed during extraction, reduces extraction cost.
Description
Technical field
The present invention relates to the extracting method of flavone component in leaf of Moringa.
Background technology
Moringa (moriga oleifera) category Moringaceae, Moringa, the sub- Himalaya mountain region originating from north India
Band.India and African country are usually used in treating the diseases such as diabetes, hypertension, cardiovascular disease, obesity.In recent years, Moringa exists
There is introducing and planting in China Guangdong, Yunnan, Hainan and Sichuan etc..Leaf of Moringa in 2012 is approved for new resource food, various peppery
Wood products exploitation is burning hot, is widely used in the fields such as food, health products, purification of water quality, cosmetics.
Chen Ruijiao etc. is using 70% ethanol as Extraction solvent, 60 DEG C, extraction time 1h of Extracting temperature, extracts 3 times, in extract solution
Flavones content reach 3.5% (Chen Ruijiao etc., the research of total flavones of Moringa oleifera leaf ethanol extraction process, food research and development [J],
2007,28(4):29-31).Sun Mingyan etc., using 70% ethanol as Extraction solvent, 80 DEG C, extraction time 1.5h of Extracting temperature, extraction time
Number 3 times, general flavone content reaches 6.593% (Sun Mingyan etc., the optimizing research of total flavones of Moringa oleifera leaf extracting method, Shaoguan in extract solution
Institute's journal [J], 2007,28 (12)：88-92).However, it is found by the inventors that being grown using the time of refluxing extraction, energy consumption cost is higher.
Microwave abstracting is the temperature of cell interior is increased rapidly using microwave energy, and cell interior pressure, which increases, breaks cell
Split, active ingredient flows out in medicinal material, and is dissolved in extract at a lower temperature.This method homogeneous heating, the thermal efficiency is higher, carries
Take speed fast.It yet there are no the relevant report extracted using microwave-assisted extraction technique to total flavones of Moringa oleifera leaf.
The content of the invention
It is an object of the invention to provide a kind of microwave extract method of flavone component in leaf of Moringa.
Specifically, the invention provides the extracting method of flavone component in leaf of Moringa, it includes following operating procedure：
Leaf of Moringa powder is taken using 50~60%v/v ethanol as solvent, solid-liquid ratio 1:50~60g/ml, microwave abstracting is carried out,
Collect extract solution, you can；Wherein, microwave power is 300~400W, and extraction time is 6~10min.
Present invention research shows that each extraction conditions parameter has a significant impact to extraction efficiency：
(1) for volume fraction of ethanol in 50-60%, extracted amount is larger, and when concentration of alcohol is further added by, extracted amount reduces.
(2) upon extracting between about 8min when, general flavone yield highest, extraction time is slightly shorter, and extraction is incomplete, and the time
Long, part flavone compound is likely to be broken, and causes yield to decline.
(3) with extraction power increase, the increase of general flavone yield, highest when power reaches about 400w, during higher than 400w then
Decline.During Microwave Extraction, electromagnetic wave is directly delivered to energy inside raw material, is allowed to instantaneously heat, when extraction power
It is too high, make flavone compound decomposition or isomerization in leaf of Moringa, cause the reduction of general flavone yield.
(4) solid-liquid ratio is from 1:30 to 1:During 60, general flavone yield is gradually stepped up, and solvent load is further added by, total yellow
Ketone yield gradually reduces.
Further, concentration of alcohol is 58~60%v/v, solid-liquid ratio 1：58~60g/ml, microwave power be 390~
400W, extraction time are 8.0~8.5min.
Further, concentration of alcohol is 58.45~60%v/v, solid-liquid ratio 1：59~60g/ml, microwave power are
397.15~400W, extraction time are 8.13~8.50min.
Preferably, concentration of alcohol 58.45%v/v, solid-liquid ratio 1：59g/ml, microwave power 397.15W, during extraction
Between be 8.13min.
In the specific embodiment of the present invention, in order to avoid influence of the low polarity component to extracting flavonoids result because
Element, so as to accurately judge that extraction process is good and bad, ungrease treatment have selected to leaf of Moringa powder before microwave abstracting.Certainly,
Even if without using ungrease treatment, currently preferred preferred plan, the microwave abstracting of still most suitable leaf of Moringa flavones.
Wherein, the method for ungrease treatment is as follows：
Leaf of Moringa powder is taken, using petroleum ether or ether as solvent extraction, the leaf of Moringa powder after extraction is taken, volatilizes solvent, you can.
Further, the solvent is petroleum ether.
Preferably, the petroleum ether boiling range is 60-90 DEG C.
Further, extracting method is selected from surname extraction.
Preferably, the surname extraction time is 8h.
Present invention also offers moringa oleifera leaf extractive prepared by said extracted method.
Present invention research is found, using identical leaf of Moringa powder as sample, total flavones of Moringa oleifera leaf obtained by the method for microwave abstracting contains
Amount is higher than reflux extraction method, while substantially reduces extraction time, has saved the water and electricity consumed during extraction, reduces extraction cost.
Brief description of the drawings
Influence of Fig. 1 concentration of alcohol to total flavones of Moringa oleifera leaf content
Influence of Fig. 2 extraction times to total flavones of Moringa oleifera leaf content
Influence of Fig. 3 microwave powers to total flavones of Moringa oleifera leaf content
Influence of Fig. 4 solid-liquid ratios to total flavones of Moringa oleifera leaf content
Fig. 5 leaf of Moringa microwave abstracting responds surface curve and 3D figures
Embodiment
1 extracting method of the present invention of embodiment
Leaf of Moringa powder is taken using 58.45%v/v ethanol as solvent, solid-liquid ratio 1:59g/ml, carries out microwave abstracting, and collection carries
Take liquid, you can；Wherein, microwave power 397.15W, extraction time 8.13min.
2 extracting method of the present invention of embodiment
Leaf of Moringa powder is taken using 60%v/v ethanol as solvent, solid-liquid ratio 1:60g/ml, microwave abstracting is carried out, collect extraction
Liquid, you can；Wherein, microwave power 400W, extraction time 8.5min.
3 extracting method of the present invention of embodiment
Leaf of Moringa powder is taken using 58%v/v ethanol as solvent, solid-liquid ratio 1:58g/ml, microwave abstracting is carried out, collect extraction
Liquid, you can；Wherein, microwave power 390W, extraction time 8.0min.
4 extracting method of the present invention of embodiment
Leaf of Moringa powder is taken, with the cable-styled backflow ungrease treatment 8h of petroleum ether, the leaf of Moringa powder after extraction is taken, volatilizes solvent, then
Using 58.45%v/v ethanol as solvent, solid-liquid ratio 1:59g/ml, microwave abstracting is carried out, collects extract solution, you can；Wherein, it is micro-
Wave power is 397.15W, extraction time 8.13min.
The technique of 5 extracting method of the present invention of embodiment is investigated
1. materials and methods
1.1 materials and instrument
Leaf of Moringa powder (moriga oleifera), purchased from Jinghong In Yunnan Province.
In order to avoid other low polarity components investigate the influence of result to extracting flavonoids technique in leaf of Moringa, the present invention is in work
Before skill investigation process, select to carry out leaf of Moringa powder ungrease treatment, and investigation experiment is carried out using with the raw material after batch processed.
The leaf of Moringa that the present invention uses, is required to ungrease treatment, can use in Chemistry for Chinese Traditional Medicine or natural plants chemistry often
Degreasing method, such as extracted with low polar solvent, low polar solvent used can be petroleum ether, ether etc..This hair
Ungrease treatment is mainly carried out using petroleum ether in bright, specific method is：
Leaf of Moringa powder is taken, with the cable-styled refluxing extraction 8h of petroleum ether, the leaf of Moringa powder after extraction is taken, volatilizes solvent, you can.Make
The boiling range of petroleum ether can be 60-90 DEG C or 30-60 DEG C.
Rutin：Products in China examines and determine institute.
The combination of computer microwave ultrasonic wave ultraviolet light catalyzes and synthesizes instrument：Beijing XiangHu Science and Technology Development Co., Ltd.'s XH-300UL types；
Ultraviolet-uisible spectrophotometer：The U.S. spectrum in Shanghai reaches Instrument Ltd., UV-1100 types；
1.2 test method
1.2.1 the foundation of standard curve
Precision weighs control substance of Rutin 10mg, puts in 50ml volumetric flasks, is settled to scale with 30% ethanol, shakes up, produce
0.2mg/mL comparison liquid.Precision draws reference substance solution 0.0,1.0,2.0,3.0,4.0,5.0ml, puts 10ml volumetric flasks respectively
In, respectively plus 70% ethanol is to 5ml, shakes up, adds 5% natrium nitrosum 0.5ml, shake up, and places 6min, adds 10% aluminum nitrate 0.5ml,
Shake up, place 6min, then add 4% sodium hydroxide to shake up to scale, place 15min, absorbance is determined under 510nm wavelength, with
Reagent blank makees reference, and gained light absorption value makees equation of linear regression with concentration.
1.2.2 refluxing extraction leaf of Moringa flavones and assay
Method one：
According to Chen Ruijiao method, the accurate weighed leaf of Moringa of degreasing sample 30g, 70% ethanol, liquid ratio 20 are added:1,
60 DEG C of refluxing extractions 3 times, each 1h.Extract solution filters, and merging filtrate, produces refluxing extraction general flavone (Flavonoid by
reflux extraction,FRE).After being cooled to room temperature, filtrate 1ml is taken, according to rutin standard solution light absorption value measure side
Method determines light absorption value, surveys 3 A that average1.General flavone content is calculated according to calibration curve equation.
Method two：
According to Sun Mingyan method, the accurate weighed leaf of Moringa of degreasing sample 30g, 70% ethanol, liquid ratio 20 are added:1,
80 DEG C of refluxing extractions 3 times, each 1.5h.Extract solution filters, and merging filtrate, produces refluxing extraction general flavone.After being cooled to room temperature,
Filtrate 1ml is taken, light absorption value is determined according to rutin standard solution extinction values determination method, surveys 3 A that average1.According to standard
Curvilinear equation calculates general flavone content.
1.2.3 microwave abstracting leaf of Moringa flavones and assay
The accurate weighed leaf of Moringa sample of degreasing, solvent is added by the condition of experimental design, is put in Microwave Extraction instrument, instrument
It is set as microwave power constant-mode, ultrasonic power 200w, magnetic agitation.Filtered after the completion of reaction, it is total to produce microwave abstracting
Flavones (Flavonoid by microwave extraction, FME).Filtrate 1ml is taken after being cooled to room temperature, according to survey rutin mark
Quasi- product solution light absorption value method determines light absorption value, surveys 3 A2 that average.General flavone content is calculated according to calibration curve equation.
1.2.4 leaf of Moringa flavones yield is calculated according to standard curve, and flavones mass concentration C, Moringa are calculated by light absorption value A
Leaf flavonoids yield F is then calculated by following formula：
F1=C1×10×V1/M1……(1)
Formula (1) is refluxing extraction general flavone yield calculation formula：C1For flavones mass concentration mg/ml；10 be 10 times；V1For
Filtrate volume；M1For sample quality；
F2=C2×10×50/M2……(2)
Formula (2) is microwave abstracting general flavone yield calculation formula：C2For flavones mass concentration mg/ml；10 be 10 times；50 are
50ml, M2For sample quality.
1.2.5 response phase method Optimal Experimental
On the basis of experiment of single factor, become by investigation of factors such as determining alcohol, microwave power, extraction time, liquid ratios
Amount, using general flavone yield F as response, using Design-Expert8.0.6 softwares, optimize Microwave Extraction total flavones of Moringa oleifera leaf
Obtain process conditions.Empirical factor is shown in Table 1. with level design
The Box-Behnken contrived experiment factor levels of table 1
2 results and analysis
2.1 equation of linear regression
According to light absorption value corresponding to each concentration rutin, linear regression C=0.0817A+0.0018, coefficient correlation is calculated
R2=0.9998.
2.2 refluxing extraction Moringa leaf flavone contents
Method one：Extract solution filtering after backflow, average absorbance value 0.499, volume 1575ml are bent according to standard
Line computation obtains general flavone content as 2.05%.
Method two：Extract solution filtering after backflow, average absorbance value 0.375, volume 1590ml are bent according to standard
Line computation obtains general flavone content as 1.72%.
2.2 experiment of single factor
2.2.1 the influence of concentration of alcohol accurately weighs 5 parts of 1g leaf of Moringa powder, be separately added into 50%, 60%, 70%, 80%,
90% ethanol Microwave Extraction, microwave power 200w, extraction time 6min, liquid ratio 50:1, as a result see Fig. 1.
As seen from Figure 1, volume fraction of ethanol is in 50-70%, and extracted amount is maximum, and when concentration of alcohol is further added by, extraction
Amount reduces.Therefore selection volume fraction is 60%.
2.2.2 the influence of extraction time accurately weighs 5 parts of 1g leaf of Moringa powder, adds 60% ethanol, microwave power 200w, liquid
Expect ratio 50:1,6min, 8min, 10min, 12min, 14min are extracted respectively.As a result Fig. 2 is seen.
From Figure 2 it can be seen that when being 8min between upon extracting, general flavone yield highest, during less than 8min, extraction is incomplete, and
During higher than 8min, part flavone compound is likely to be broken, and causes yield to decline.Therefore, the selective extraction time is 8min.
2.2.3 the influence of microwave power accurately weighs 5 parts of 1g leaf of Moringa powder, adds 60% ethanol, liquid ratio 50:1, respectively
In microwave power 100w, 200w, 300w, 400w, 500w, 8min is extracted.As a result Fig. 3 is seen.
As seen from Figure 3, with extraction power increase, the increase of general flavone yield, highest when power reaches 400w, higher than 400w
When then decline.During Microwave Extraction, electromagnetic wave is directly delivered to energy inside raw material, is allowed to instantaneously heat, when extraction work(
Rate is too high, makes flavone compound decomposition or isomerization, causes the reduction of general flavone yield.Therefore, selective extraction power is 400w.
2.2.4 the influence of solid-liquid ratio takes 60% ethanol 50ml, according to 1:70、1:60、1:50、1:40、1:30 add Moringa
Ye Fen, microwave power 400w, 8min is extracted, as a result sees Fig. 4.
From fig. 4, it can be seen that when solid-liquid ratio is 1:When 60, general flavone yield highest, therefore it is 1 to select solid-liquid ratio:60.
Optimization of 2.3 response phase methods to total flavones of Moringa oleifera leaf Microwave Extraction technological parameter
Analysis of experiments scheme and it the results are shown in Table 2
The testing program of table 2 and result
Gained test data is subjected to multiple regression fitting, leaf of Moringa flavones yield Y is obtained and becomes with each factor of Microwave Extraction
The quadratic regression equation model of amount is：
Y=3.48-0.087X1-.0.11X2+0.039X3+0.012X4-0.05X1X2+0.027X1X3+0.057X1X4-
0.14X2X3-0.1X2X4+0.006536X3X4-0.27X1 2-0.25X2 2-0.29X3 2-0.26X4 2
In order to examine the validity of equation, mathematical modeling is obtained to Microwave Extraction total flavones of Moringa oleifera leaf and carries out variance analysis, and
The deviation regression coefficient of each factor is tested, the results are shown in Table 3
The regression model variance analysis of table 3
Note：" * " is significant difference (p ＜ 0.05), and " * * " are that difference height is extremely notable (p ＜ 0.01), and " * * * " are difference pole
Significantly (p ＜ 0.001).
As shown in Table 3, model (P values<0.0001) it is very significant, model, which loses, intends item expression model predication value and reality
The probability (P=0.1049) that value is not fitted is inapparent, and model selection is suitable.Regression coefficient R2=0.9852, more than 0.9,
Illustrate that the model degree of correlation is fine.So the change of response can be analyzed using the model.
In order to further study correlated variables reciprocation and determine optimum point, painted by Design Expert softwares
Response surface curve map processed carries out visual analyzing.As a result Fig. 5 are seen
Schemed from Fig. 5 response surfaces 3D, response has maximum, calculated through software analysis, and it is pre- to obtain general flavone content
Packet concentration during measured value maximum：Determining alcohol 58.45%, microwave power 397.15w, extraction time 8.13min, solid-liquid ratio 1:
59.5 checking tests are carried out to result of the test, the results showed that, it is 3.45% that general flavone, which obtains average yield, with theoretical expectation values base
This coincide.
Using identical leaf of Moringa powder as sample, total flavones of Moringa oleifera leaf content obtained by the method for microwave abstracting is higher than refluxing extraction
Method, while extraction time is substantially reduced, the water and electricity consumed during extraction has been saved, has reduced extraction cost.
Claims (7)
1. the extracting method of flavone component in leaf of Moringa, it is characterised in that：It includes following operating procedure：
Leaf of Moringa powder is taken using 60%v/v ethanol as solvent, solid-liquid ratio 1：60g/ml, microwave abstracting is carried out, collect extract solution, i.e.,
Can；Wherein, microwave power 400W, extraction time 8min.
2. extracting method according to claim 1, it is characterised in that：Before microwave abstracting, degreasing is carried out to leaf of Moringa powder
Processing, specific method are as follows：
Leaf of Moringa powder is taken, using petroleum ether or ether as solvent extraction, the leaf of Moringa powder after extraction is taken, volatilizes solvent, you can.
3. extracting method according to claim 2, it is characterised in that：The solvent is petroleum ether.
4. extracting method according to claim 3, it is characterised in that：The petroleum ether boiling range is 60-90 DEG C.
5. extracting method according to claim 2, it is characterised in that：Extracting method is selected from surname extraction.
6. extracting method according to claim 5, it is characterised in that：The surname extraction time is 8h.
7. moringa oleifera leaf extractive prepared by extracting method described in claim 1~6 any one.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410366619.4A CN104173393B (en) | 2014-07-29 | 2014-07-29 | The extracting method of flavone component in leaf of Moringa |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410366619.4A CN104173393B (en) | 2014-07-29 | 2014-07-29 | The extracting method of flavone component in leaf of Moringa |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104173393A CN104173393A (en) | 2014-12-03 |
| CN104173393B true CN104173393B (en) | 2017-11-24 |
Family
ID=51954917
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410366619.4A Active CN104173393B (en) | 2014-07-29 | 2014-07-29 | The extracting method of flavone component in leaf of Moringa |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104173393B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104758325A (en) * | 2015-04-03 | 2015-07-08 | 萧丽雅 | Extraction method and application of moringa oleifera leaf total flavones |
| CN104971140A (en) * | 2015-07-24 | 2015-10-14 | 青岛蓝盛洋医药生物科技有限责任公司 | Medicine composition for treating bone fracture and preparation method thereof |
| CN106755266A (en) * | 2016-12-21 | 2017-05-31 | 四川农业大学 | Hypoglycemic, pancreas islet protection activity test method of Moringa leaf flavone extract and application thereof |
| CN111568934A (en) * | 2020-04-28 | 2020-08-25 | 五邑大学 | Method for extracting moringa oleifera total flavonoids by using deep eutectic solvent |
| CN116459286B (en) * | 2023-06-07 | 2024-05-14 | 河北瑞龙生物科技有限公司 | Method for extracting moringa flavone from moringa leaves |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1745655A (en) * | 2004-09-09 | 2006-03-15 | 林保村 | Moringaceae powdery, its preparation, and use of capsules and tablets |
| CN102669423A (en) * | 2012-05-28 | 2012-09-19 | 韩金光 | Moringa extract as well as preparation method of moringa extract and moringa feed additive |
-
2014
- 2014-07-29 CN CN201410366619.4A patent/CN104173393B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1745655A (en) * | 2004-09-09 | 2006-03-15 | 林保村 | Moringaceae powdery, its preparation, and use of capsules and tablets |
| CN102669423A (en) * | 2012-05-28 | 2012-09-19 | 韩金光 | Moringa extract as well as preparation method of moringa extract and moringa feed additive |
Non-Patent Citations (3)
| Title |
|---|
| 微波萃取技术在提取黄酮类化合物中的应用;邓丽芳;《西安文理学院学报：自然科学版》;20131231;16-21 * |
| 辣木叶总黄酮乙醇提取工艺的研究;陈瑞娇等;《食品研究与开发》;20070405(第04期);29-31 * |
| 辣木叶总黄酮的提取及其降血糖作用;陈瑞娇等;《食品与生物技术学报》;20070715(第04期);42-45 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104173393A (en) | 2014-12-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104173393B (en) | The extracting method of flavone component in leaf of Moringa | |
| CN105301089B (en) | A kind of Strontium Isotopic Ratios measured in strawberry tree different parts sample and the method for identifying the red bayberry place of production | |
| CN104833654A (en) | Method and application for rapidly detecting mother chrysanthemum medicinal materials by utilizing near infrared spectroscopy | |
| CN106124648B (en) | Method that is a kind of while extracting and measure western three enediol of cypress of tobacco, vitamin E, phytosterol | |
| CN101816842A (en) | Far infrared radiation auxiliary extracting system | |
| CN102106892B (en) | Process for preparing Chinese herbal slices of dogwood fruit pulp prepared for decoction | |
| CN105784827A (en) | Method for measuring content of nine heavy metals in sugarcane juice with electric heating plate digestion/ICP-MS method | |
| CN106198791B (en) | Method that is a kind of while measuring agalloch eaglewood tetrol and benzylacetone in agalloch eaglewood | |
| CN104122241A (en) | Method for rapid determination of selenium content in tetrastigmatis hemsleyani | |
| CN107029038A (en) | A kind of response phase method optimizes the extraction process of longan leaf flavonoids | |
| CN103792326B (en) | A kind of method evaluating tea dust mixing uniformity | |
| CN105885469A (en) | Process for extracting lonicera caerulea anthocyanins through acidized ethanol under cooperative assistance of ultrasonic waves-microwaves | |
| CN109134583A (en) | The method of triterpene compound is extracted from schefflera leaf using ionic liquid | |
| CN102759587A (en) | Detection method of residual amount of maleic hydrazide in tobacco and tobacco products | |
| CN102692385B (en) | Method for determining talcum powder in cosmetic | |
| CN102590149A (en) | Method for fast detecting content of aspartic acid in cordyceps sinensis powder | |
| CN102813679B (en) | Method and equipment for sulphur fumigation of traditional Chinese medicine materials | |
| CN110269872A (en) | A kind of Chinese medicine teasel root processed product, concocting method and detection method | |
| CN102175640A (en) | Near-infrared online detecting method in herba epimedii processing production | |
| CN108452035A (en) | A kind of extracting method of peony petal polyphenol | |
| CN111610280A (en) | Optimization method of extraction process of taxane compounds in taxus chinensis | |
| CN110141620A (en) | It is a kind of that shiyacha total flavone method is extracted using cellulase-microwave combination technique | |
| CN106770010A (en) | A kind of weisu granules extract solution concentration process On-line near infrared analyzer detection method | |
| Wang et al. | Optimization of microwave assisted extraction conditions for total flavonoids in toona sinensis leaves using response surface methodology | |
| CN115124587B (en) | Method for extracting tea saponin from tea vinasse and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20211125 Address after: No.9, Jinxin Road, Yueyang hi tech Industrial Park, rongjiawan Town, Yueyang County, Yueyang City, Hunan Province, 414000 Patentee after: YUEYANG YUMEIKANG BIO-TECH Co.,Ltd. Address before: Ya'an City, Sichuan Province, 625014 new Yucheng Kang Road No. 46 Patentee before: SICHUAN AGRICULTURAL University |
|
| TR01 | Transfer of patent right |